Publications by authors named "Pengfei Li"

801 Publications

Synthesis of a pH-responsive nano-cellulose/sodium alginate/MOFs hydrogel and its application in the regulation of water and N-fertilizer.

Int J Biol Macromol 2021 Jul 24;187:262-271. Epub 2021 Jul 24.

Institute of Chemical Industry of Forestry Products, Chinese Academy of Forestry, Key Laboratory of Biomass Energy and Material, National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration, Nanjing 210042, Jiangsu Province, China. Electronic address:

In order to circumvent the water eutrophication caused by nitrogen loss in agriculture, slow-release and high-water containing fertilizers have captured much attention. Considering the unstable release of traditional slow-released fertilizers, novel strategies need to be designed to meet the steady release of fertilizers. Herein, by integrating cellulose-based hydrogel with MIL-100(Fe), a pH-sensitive Cellulose/MOFs hydrogel (CAM) with a high surface area (45.25 m/g) was devised. The volume changes and the water adsorption of the hydrogels were uncovered from pH 3 to pH 11, where the highest water adsorption (100 g/g) was achieved at pH 11. Besides, a pH-sensitive urea slow release fertilizer (U-CAM) was also designed. The urea release of the U-CAM at pH 11 was much slower than that of the U-CAM at pH 3, which indicated its potential application in arid regions. In parallel with a favorable water-holding capacity, the totally loss of the soil moisture loaded with U-CAM was slowed down by 18 days as compared with the pure soil. The positive effect of the U-CAM on the growth of wheat was indexed with the germination rate, number of tillers, photosynthetic rate and chlorophyll content of the crop, which verified their further application in irrigating farming.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.154DOI Listing
July 2021

Quantitative HPLC-MS/MS determination of Nuc, the active metabolite of remdesivir, and its pharmacokinetics in rat.

Anal Bioanal Chem 2021 Jul 24. Epub 2021 Jul 24.

Department of Pharmacy, Beijing Chao-Yang Hospital, Capital Medical University, No.8 Gongti South Road, Chaoyang District, Beijing, 100020, People's Republic of China.

Remdesivir is a nucleotide analog prodrug that has received much attention since the outbreak of the COVID-19 pandemic in December 2019. GS-441524 (Nuc) is the active metabolite of remdesivir and plays a pivotal role in the clinical treatment of COVID-19. Here, a robust HPLC-MS/MS method was developed to determine Nuc concentrations in rat plasma samples after a one-step protein precipitation process. Chromatographic separation was accomplished on Waters XBrige C column (50 × 2.1 mm, 3.5 μm) under gradient elution conditions. Multiple reaction monitoring transitions in electrospray positive ion mode were m/z 292.2 → 163.2 for Nuc and 237.1 → 194.1 for the internal standard (carbamazepine). The quantitative analysis method was fully validated in line with the United States Food and Drug Administration guidelines. The linearity, accuracy and precision, matrix effect, recovery, and stability results met the requirements of the guidelines. Uncertainty of measurement and incurred sample reanalysis were analyzed to further ensure the robustness and reproducibility of the method. This optimized method was successfully applied in a rat pharmacokinetics study of remdesivir (intravenously administration, 5 mg kg). The method can act as a basis for further pharmacokinetic and clinical efficacy investigations in patients with COVID-19. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-021-03561-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302467PMC
July 2021

Analysis on the Seismic Performance of Steel Fiber-Reinforced High-Strength Concrete Beam-Column Joints.

Materials (Basel) 2021 Jul 18;14(14). Epub 2021 Jul 18.

School of Civil Engineering and Architecture, Zhengzhou University of Aeronautics, Zhengzhou 450046, China.

The present research study aims to investigate numerically the behavior of steel fiber-reinforced high-strength concrete (SFRHC) beam-column joints (BCJs) under seismic action. Based on the plastic damage constitutive model of concrete and elastic-plastic mixed-strengthen constitutive model of steel material, the finite element software ABAQUS was utilized to establish the 3D finite element (FE) model of BCJs. Additionally, the feasibility and accuracy of the numerical simulation were verified by comparing the computed results and experimental observations in terms of the hysteresis curves, skeleton curves, and failure mode. Furthermore, based on the validated FE modeling approach, load vs. displacement hysteresis curves of SFRHC-BCJs during the loading process were analyzed in detail; the failure process was also investigated. Furthermore, the effect of various parameters on the seismic behavior of BCJs was analyzed comprehensively, including the concrete strength, the volume ratio of steel fiber, and the stirrup ratio in the core area. Finally, parametric studies illustrated that increasing the concrete strength helps in enhancing the ultimate load, while the ductility decreased noticeably. Both adding the steel fiber and increasing the stirrup ratio can significantly improve the seismic performance of BCJs.
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http://dx.doi.org/10.3390/ma14144016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307214PMC
July 2021

Construction of three-dimensional MgInS nanoflowers/two-dimensional oxygen-doped g-CN nanosheets direct Z-scheme heterojunctions for efficient Cr(VI) reduction: Insight into the role of superoxide radicals.

J Hazard Mater 2021 Jul 2;420:126567. Epub 2021 Jul 2.

Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

Environmental crisis aroused from carcinogenic and mutagenic heavy metal Cr(VI)-containing wastewater has attracted great attention. Herein, a direct Z-scheme three-dimensional MgInS nanoflowers/two-dimensional oxygen-doped g-CN nanosheets heterostructured composite photocatalysts were fabricated for Cr(VI) photoreduction. The crystalline structure, morphology, specific surface areas, chemical components, optical properties, as well as photoelectrochemical performance of the fabricated photocatalyst were systematically studied. All the hybrids photocatalysts exhibited superior Cr(VI) photoreduction performance than a single component. The optimal sample showed 2.0 and 343.7 times increasing than three-dimensional MgInS nanoflowers and two-dimensional oxygen-doped g-CN nanosheets, respectively. The enhancement of the Cr(VI) photoreduction performance could be ascribed to the enhanced specific surface areas, the improved light absorption, and the Z-scheme charge carriers' separation and migration pathway. Moreover, the role of superoxide radicals was investigated. It is speculated that this research would present a new sight toward photocatalytic Cr(VI) reduction.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126567DOI Listing
July 2021

Efficacy of apatinib combined with tegafur gimeracil and oteracil potassium in the second-line treatment of advanced gastric cancer.

J BUON 2021 May-Jun;26(3):917-923

Department of Pharmacy, Beijing Chao-yang Hospital, Capital Medical University, Beijing, China.

Purpose: To investigate the clinical efficacy and safety of apatinib combined with tegafur-gimeracil-oteracil potassium (S-1) in the second-line treatment of advanced gastric cancer.

Methods: A total of 126 patients with advanced gastric cancer admitted to our hospital from January 2017 to September 2018 were enrolled as the research objects, none of whom underwent surgery. For these patients, second-line treatment was recommended due to the failure of first-line treatment. According to the different therapeutic options, patients were categorized into S-1 group (n=63) and Apatinib group (S-1 combined with apatinib, n=63), and drugs were administered orally. The clinical efficacy, serological indicators, adverse reactions and immune function were compared between the two groups. Besides, the survival status of patients was recorded through follow-up.

Results: In S-1 group and Apatinib group, the objective response rate (ORR) was 30.2% (19/63) vs. 50.8% (32/63) and the disease control rate (DCR) was 54.0% (34/63) vs. 74.6% (47/63), respectively. The results indicated that Apatinib group was superior to S-1 group in terms of ORR and DCR, suggesting statistically significant differences (p=0.018, p=0.016). Compared with those before treatment, the serum levels of carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA), and tumor supplied group of factors (TSGF) in the two groups of patients were prominently reduced after treatment (p<0.05). After treatment, CA19-9 and TSGF levels were remarkably lower in Apatinib group than those in S-1 group (p=0.008, p=0.023), and there was no statistically significant difference in the CEA level between the two groups (p=0.057). After treatment, the quality of life of patients was improved notably in Apatinib group compared with that in S-1 group (p=0.002). Adverse reactions mainly involved hematological toxicity, nausea and vomiting, abnormal renal function, impairment of hepatic function, neurotoxicity, hypertension and hand-foot syndrome, most of which were in grade I-II and relieved after symptomatic treatment and could be tolerated for continued treatment. Serious adverse reactions in grade III-IV occurred rarely. No statistically significant difference was found in the incidence rate of adverse reactions between the two groups of patients (p>0.05). Besides, according to the follow-up results, median overall survival (OS) was 8.1 months vs. 10.7 months and median progression-free survival (PFS) was 4.2 months vs. 5.3 months, respectively, in S-1 group and Apatinib group. The results of log-rank test demonstrated that Apatinib group was superior to S-1 group with respect to OS, showing a statistically significant difference (p=0.028), and no statistically significant difference was found in PFS between the two groups of patients (p=0.159).

Conclusion: In the second-line treatment of advanced gastric cancer, apatinib combined with S-1 is superior to S-1 alone in term of clinical efficacy, and its adverse reactions can be tolerated. Apatinib combined with S-1 can prominently improve the quality of life, reduce the serum tumor marker levels and prolong the OS of patients, but it cannot extend the PFS.
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July 2021

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages.

Front Immunol 2021 29;12:700009. Epub 2021 Jun 29.

College of Life Science, Northwest University, Xi'an, China.

Macrophages can be polarized into classically activated macrophages (M1) and alternatively activated macrophages (M2) in the immune system, performing pro-inflammatory and anti-inflammatory functions, respectively. Human THP-1 and mouse RAW264.7 cell line models have been widely used in various macrophage-associated studies, while the similarities and differences in protein expression profiles between the two macrophage models are still largely unclear. In this study, the protein expression profiles of M1 and M2 phenotypes from both THP-1 and RAW264.7 macrophages were systematically investigated using mass spectrometry-based proteomics. By quantitatively analyzing more than 5,000 proteins among different types of macrophages (M0, M1 and M2) from both cell lines, we identified a list of proteins that were uniquely up-regulated in each macrophage type and further confirmed 43 proteins that were commonly up-regulated in M1 macrophages of both cell lines. These results revealed considerable divergences of each polarization type between THP-1 and RAW264.7 macrophages. Moreover, the mRNA and protein expression of CMPK2, RSAD2, DDX58, and DHX58 were strongly up-regulated in M1 macrophages for both macrophage models. These data can serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.
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http://dx.doi.org/10.3389/fimmu.2021.700009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276023PMC
June 2021

Exploring the metabolic characteristics and pharmacokinetic variation of paroxetine in healthy volunteers using a pharmacometabonomic approach.

J Pharm Biomed Anal 2021 Jun 24;204:114224. Epub 2021 Jun 24.

Pharmacy Department of Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, PR China. Electronic address:

The pharmacokinetic parameters of paroxetine vary between individuals, leading to differences in efficacy. The focus of our research was to predict responses to paroxetine using a pharmacometabonomic approach combining metabolic phenotypes and pharmacokinetic parameters. The pharmacokinetics of 12 healthy volunteers treated with paroxetine over two cycles was determined using high-performance liquid chromatography tandem mass spectrometry. Metabolic profiling and phenotyping were performed on the blood samples that remained after pharmacokinetic studies, using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry. Thirty-nine metabolites (p < 0.05) increased or decreased after treatment with paroxetine. Vitamin B6 metabolism; valine, leucine, and isoleucine biosynthesis; phenylalanine metabolism; pantothenate and coenzyme A biosynthesis; tyrosine metabolism; and glyoxylate and dicarboxylate metabolism were likely to be relevant for the effects of paroxetine. The two-stage partial least squares (PLS) strategy was used to screen potential biomarkers and predict the pharmacokinetic parameters of paroxetine. An orthogonal PLS discriminant analysis strategy was then applied to separate the high- and low-value groups based on the screened biomarkers. Pearson correlation test and receiver operating characteristic curve analysis confirmed the key prediction biomarkers. Seven common biomarkers were able to predict both the area under the curve (AUC) and the maximum concentration (C): cortisone, l-tyrosine, phenylpyruvate, l-valine, 2-oxoglutarate, l-lactate, and glycerate. Furthermore, homoprotocatechuate and l-glutamate were unique biomarkers for AUC, and citicoline and fumarate were unique biomarkers for C. The selected biomarkers were able to predict the AUC and C and discriminate good responders from poor responders to paroxetine treatment. This trial was registered with http://www.chinadrugtrials.org.cn/ (no. CTR20171590).
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http://dx.doi.org/10.1016/j.jpba.2021.114224DOI Listing
June 2021

Urbanization, ambient air pollution, and prevalence of chronic kidney disease: A nationwide cross-sectional study.

Environ Int 2021 Jul 10;156:106752. Epub 2021 Jul 10.

Renal Division, Department of Medicine, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China; National Institutes of Health Data Science at Peking University, Beijing 100191, China. Electronic address:

An increasing number of studies have linked ambient air pollution to chronic kidney disease (CKD) prevalence. However, its potential effect modification by urbanization has not been investigated. Based on data of 47,204 adults from the China National Survey of Chronic Kidney Disease (CKSCKD) dataset, night light satellite remote sensing data and high-resolution air pollution inversion products, the present cross-sectional study investigated the association between fine particulate matter <2.5 mm in diameter (PM), nitrogen dioxide (NO), night light index (NLI) and CKD prevalence in China, and the effect modification by urbanization characterized by administrative classification and NLI on the pollutant-health associations. Our results showed that a 10-μg/m increase in PM at 3-year moving average, a 10-μg/m increase in NO at 5-year moving average, and a 10-U increase in NLI at 5-year moving average were significantly associated with increased odds of CKD prevalence [OR = 1.24 (95 %CI:1.14, 1.35); OR = 1.12 (95 %CI:1.09, 1.15); OR = 1.05 (95 %CI:1.02, 1.07)]. Meanwhile, the pollutant-health associations were more apparent in medium-urbanized areas compared to low- and high-urbanized areas. For instance, a 10-μg/m increase in PM concentration at 2-year moving average was associated with increased odds of CKD in the areas with NLI level in the second [OR = 2.78 (95 %CI:1.77, 4.36)] and third quartiles [OR = 1.49 (95 %CI:1.14, 1.95)], compared to the lowest [OR = 0.96 (95% CI: 0.73, 1.26)] and highest [OR = 0.63 (95% CI: 0.39-1.02)] quartiles. PM and NO were associated with increased odds of CKD prevalence, especially in areas with medium NLI levels, suggesting the necessity of strengthening environmental management in medium-urbanized regions.
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http://dx.doi.org/10.1016/j.envint.2021.106752DOI Listing
July 2021

Renin-angiotensin system inhibitor is associated with the reduced risk of all-cause mortality in COVID-19 among patients with/without hypertension.

Front Med 2021 Jul 9. Epub 2021 Jul 9.

Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital; Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing, 100191, China.

Consecutively hospitalized patients with confirmed coronavirus disease 2019 (COVID-19) in Wuhan, China were retrospectively enrolled from January 2020 to March 2020 to investigate the association between the use of renin-angiotensin system inhibitor (RAS-I) and the outcome of this disease. Associations between the use of RAS-I (angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB)), ACEI, and ARB and in-hospital mortality were analyzed using multivariate Cox proportional hazards regression models in overall and subgroup of hypertension status. A total of 2771 patients with COVID-19 were included, with moderate and severe cases accounting for 45.0% and 36.5%, respectively. A total of 195 (7.0%) patients died. RAS-I (hazard ratio (HR)= 0.499, 95% confidence interval (CI) 0.325-0.767) and ARB (HR = 0.410, 95% CI 0.240-0.700) use was associated with a reduced risk of all-cause mortality among patients with COVID-19. For patients with hypertension, RAS-I and ARB applications were also associated with a reduced risk of mortality with HR of 0.352 (95% CI 0.162-0.764) and 0.279 (95% CI 0.115-0.677), respectively. RAS-I exhibited protective effects on the survival outcome of COVID-19. ARB use was associated with a reduced risk of all-cause mortality among patients with COVID-19.
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http://dx.doi.org/10.1007/s11684-021-0850-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267232PMC
July 2021

The cadmium toxicity in gills of Mytilus coruscus was accentuated by benzo(a)pyrene of higher dose but not lower dose.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Jul 5;249:109128. Epub 2021 Jul 5.

Key Laboratory of Sustainable Utilization of Technology Research, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds, MOA, Zhejiang Marine Fisheries Research Institute, Zhejiang, Zhoushan 316021, China.

In natural environment, the existence of interactions of toxic mixtures could induce diverse biochemical pathways and consequently exert different toxicological responses in aquatic organisms. However, little information is available on the effects of combined xenobiotics on lower aquatic invertebrates. Here, we assessed the effects of cadmium (Cd, 0.31 mg/L) as well as the mixture of Cd (0.31 mg/L) and benzo(a)pyrene (Bap, 5 or 50 μg/L) on bioaccumulation, antioxidant, lipid peroxidation (LPO) and metallothionein (MT) responses in gills of thick shell mussel Mytilus coruscus. Upon exposed to single Cd, the metal bioaccumulation, antioxidant enzymes activities, LPO and MT level significantly increased in the gills, suggesting an apparent toxicity to mussels. The interaction of Cd + 5 μg/L Bap did not significantly alter these endpoints compared to single Cd. However, once the dose of Bap elevated to 50 μg/L, the induction of bioaccumulation, antioxidant system and LPO was even more pronounced while the induction of MT was remarkably inhibited, implying an accentuated toxicity. Collectively, the current results demonstrated that 0.31 mg/L Cd exposure resulted in severe toxicity to mussels despite of the induction of MT system to alleviate the metal toxicity. Once the Cd exposure combined with Bap, the lower dose of Bap could not change the Cd toxicity while the higher dose of Bap accentuated the toxicity by inhibiting metallothionein synthesis. These findings might provide some useful clues for elucidation the mechanism of the interaction of combined xenobiotics in molluscs.
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http://dx.doi.org/10.1016/j.cbpc.2021.109128DOI Listing
July 2021

ZEB2 facilitates peritoneal metastasis by regulating the invasiveness and tumorigenesis of cancer stem-like cells in high-grade serous ovarian cancers.

Oncogene 2021 Jul 1. Epub 2021 Jul 1.

Department of Gynecology, Obstetrics & Gynecology Hospital, Fudan University, Shanghai, 200011, China.

Peritoneal metastasis is a common issue in the progression of high-grade serous ovarian cancers (HGSOCs), yet the underlying mechanism remains unconfirmed. We demonstrated that ZEB2, the transcription factor of epithelial-mesenchymal transition (EMT), was upregulated in ascites cells from HGSOC patients and in CD133 cancer stem-like cells (CSLCs) from epithelial ovarian cancer (EOC) cell lines. SiRNA-mediated knockdown of ZEB2 in EOC cells decreased the percentage of CSLCs and reduced the colony forming potential, cell invasion capacity and expression of pluripotent genes Oct4 and Nanog. Inhibition of ZEB2 also induced cellular apoptosis and impacted the tumorigenicity of ovarian CSLCs. The mesenchymal markers N-cadherin and vimentin were downregulated, while the epithelial marker E-cadherin was upregulated after ZEB2 knockdown. MiR-200a, a molecule that downregulates ZEB2, had the opposite effect of ZEB2 expression in EOC-CSLCs. A retrospective study of 98 HGSOC patients on the relationship of ascites volume, pelvic and abdominal metastasis, International Federation of Gynecology and Obstetrics (FIGO) stage and the malignant involvement of abdominal organs and lymph nodes was performed. Patients with high expression of ZEB2 in tumour tissues had a higher metastasis rate and a poorer prognosis than those with low expression. The parameters of ZEB2 expression and ascites volume were strongly linked with the prognostic outcome of HGSOC patients and had higher hazard ratios. These findings illustrated that ZEB2 facilitates the invasive metastasis of EOC-CSLCs and can predict peritoneal metastasis and a poor prognosis in HGSOC patients.
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http://dx.doi.org/10.1038/s41388-021-01913-3DOI Listing
July 2021

Seismic Performance of Steel Fiber Reinforced High-Strength Concrete Beam-Column Joints.

Materials (Basel) 2021 Jun 11;14(12). Epub 2021 Jun 11.

State Key Laboratory of Green Building Materials, China Building Materials Academy, Beijing 100024, China.

In high-strength concrete, the reinforcement concentration will cause some problems in the beam-column joints (BCJs) due to a large amount of transverse reinforcement. Hence, the main object of this paper is to prevent the reinforcement concentration and reduce the amount of transverse reinforcement in the BCJs through the ideal usage of steel fibers and reinforced high-strength concrete. Pseudo-static tests on seven specimens were carried out to investigate and evaluate the seismic performance of beam-column joints in steel fiber reinforced high-strength concrete (SFRHC). Test variables were steel fiber volume ratio, concrete strength, the stirrup ratio in the core area, and an axial compression ratio of the column end. During the test, the hysteresis curves and failure mode were recorded. The seismic indicators, such as energy dissipation, ductility, strength, and stiffness degradation, were determined. The experimental results indicated that the failure modes of SFRHC beam-column joints mainly included the core area failure and the beam end bending failure. With the increase in stirrup ratio, volume ratio of steel fiber, and axial compression ratio in the core area, both the ductility and energy consumption of beam-column joints increased, while the opposite was true for concrete strength.
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http://dx.doi.org/10.3390/ma14123235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231253PMC
June 2021

Symmetry-breaking bifurcations and ghost states in the fractional nonlinear Schrödinger equation with a PT-symmetric potential.

Opt Lett 2021 Jul;46(13):3267-3270

We report symmetry-breaking and restoring bifurcations of solitons in a fractional Schrödinger equation with cubic or cubic-quintic (CQ) nonlinearity and a parity-time-symmetric potential, which may be realized in optical cavities. Solitons are destabilized at the bifurcation point, and, in the case of CQ nonlinearity, the stability is restored by an inverse bifurcation. Two mutually conjugate branches of ghost states (GSs), with complex propagation constants, are created by the bifurcation, solely in the case of fractional diffraction. While GSs are not true solutions, direct simulations confirm that their shapes and results of their stability analysis provide a "blueprint" for the evolution of genuine localized modes in the system.
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http://dx.doi.org/10.1364/OL.428254DOI Listing
July 2021

Research progress of indole compounds with potential antidiabetic activity.

Eur J Med Chem 2021 Jun 23;223:113665. Epub 2021 Jun 23.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China. Electronic address:

New types of antidiabetic agents are continually needed with diabetes becoming the epidemic in the world. Indole alkaloids play an important role in natural products owing to their variable structures and versatile biological activities like anticonvulsant, anti-inflammatory, antidiabetic, antimicrobial, and anticancer activities, which are a promising source of novel antidiabetic drugs discovery. The synthesized indole derivatives possess similar properties to natural indole alkaloids. In the last two decades, more and more indole derivatives have been designed and synthesized for searching their bioactivities. This present review describes comprehensive structures of indole compounds with the potential antidiabetic activity including natural indole alkaloids and the synthetic indole derivatives based on the structure classification, summarizes their approaches isolated from natural sources or by synthetic methods, and discusses the antidiabetic effects and the mechanisms of action. Furthermore, this review also provides briefly synthetic procedures of some important indole derivatives.
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http://dx.doi.org/10.1016/j.ejmech.2021.113665DOI Listing
June 2021

Effect of heat treatment on the emission rate of radon from red sandstone.

Environ Sci Pollut Res Int 2021 Jun 29. Epub 2021 Jun 29.

College of Geology and Environment, Xi'an University of Science and Technology, Xi'an, 710054, Shaanxi, China.

High temperature and pore structure are important factors affecting the emission rate of radon in rocks. This study mainly focused on the correlation between radon emission rate and temperature in red sandstone. The results showed that in the temperature range of 25-400 °C, as the temperature increased, the connectivity of the internal pores of the sample became better, resulting in a significantly increased radon emission rate. The radon emission rate at 400 °C was 2.86 times the original. To explain the changes that occurred in the internal structure of the samples, the porosity characteristics of the samples after heat treatment were studied by nuclear magnetic resonance (NMR). It was found that the pore structure was also an important factor affecting the rate of radon emission. The smaller pore size of the micropores (r < 0.1 μm) inhibited the emission of radon in the sandstone. These results helped in understanding the mechanism of radon emission rate and provide an important basis for predicting rock fragmentation and coal fire.
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http://dx.doi.org/10.1007/s11356-021-15079-8DOI Listing
June 2021

Vertical migration of microplastics in porous media: Multiple controlling factors under wet-dry cycling.

J Hazard Mater 2021 Jun 17;419:126413. Epub 2021 Jun 17.

School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Terrestrial soils are not only a large reservoir for Microplastics (MPs), but also a possible entrance to the subsurface environment, posing potential risks to the subterranean habitats and groundwater. In this study, we examined the vertical transport of MPs of four polymers, i.e., polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP) and polyamide (PA), in porous sand media driven by wet-dry cycling. The effects of polymer properties, MP size, sand particle size, wet-dry cycles, and dissolved organic matter (DOM) on their migration behavior were investigated. Surface hydrophobicity showed a strong positive correlation with MP mobility, with PA exhibiting the greatest movement potential, followed by PE, PET, and PP. The penetration depth of MP particles increased with decreasing MP particle size (d) and increasing sand diameter (d). MP particles migrated deeper in sand media when d/d < 0.11. Furthermore, frequent wet-dry cycles and the presence of DOM promoted the vertical migration of MPs in the sand. The results revealed multiple factors influencing the vertical migration of MPs in sand, which is instructive for understanding the ecological risk of MPs in potentially contaminated soil (e.g., farmland with long-term mulching) to the subsurface environment and potential negative impact to public health.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126413DOI Listing
June 2021

High PHD Finger Protein 19 (PHF19) expression predicts poor prognosis in colorectal cancer: a retrospective study.

PeerJ 2021 1;9:e11551. Epub 2021 Jun 1.

Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Colorectal cancer (CRC) is the third most common cancer all around the world, and it seriously threats human health. PHF19 has been proved to be closely related to the prognosis of patients in a variety of malignant tumors, but the effect of PHF19 on the prognosis evaluation of CRC patients has not been confirmed.

Methods: In our study, we used GEO, TCGA database and IHC to verify the PHF19 expression in CRC samples. Survival analysis of PHF19 based on TCGA, GEO series, and our own CRC sample were performed. Cox regression was performed to reveal the relationship between PHF19 and prognosis. Co-expression was performed to find genes related to PHF19 expression. GO/KEGG enrichment analysis and GSEA analysis were used to confirm the most relevant signal pathway to PHF19. Next, cell experiments were performed to verify the effect of PHF19 on the proliferation, invasion and metastasis of CRC. Then, Western blot was used to verify the protein expression of the above two phenotypes. Finally, tumor formation experiments in nude mice were used to verify the role of PHF19 of tumor proliferation in vivo.

Results: We found that PHF19 was significantly over-expressed in tumors compared with normal tissues. Kaplan-Meier (K-M) analysis indicated that high PHF19 in CRC associated with poor overall survival (OS) in CRC patients. Clinical correlation analysis showed that high expression of PHF19 was closely related to t umor progression in CRC patients, especially infiltration and metastasis. Bioinformatics revealed that PHF19 might affect tumor malignant phenotype by regulating the cell cycle in CRC. CCK-8 and clonal formation experiment showed that the proliferative ability of tumor cells was promoted. Flow cytometry showed that the cell cycle accelerated the transition from G1 to S phase. Western blot found that Cyclin D1, CDK4, and CDK6 expression were up-regulated. Transwell and wound-healing experiment found that invasive and migratory abilities was promoted after the over-expression of PHF19. Western blot showed that the expression of key proteins of Epithelial-Mesenchymal Transition (EMT) changed. Tumor formation experiments in nude mice showed that overexpression of PHF19 could promote tumor proliferation in vivo.

Conclusion: Our research proved that PHF19 could be an independent prognostic factor for CRC, PHF19 promoted the proliferative ability and the invasion and metastasis of CRC by up-regulating the expression of key molecules related to cell cycle and EMT pathway in vitro, promoting tumor proliferation in vivo.
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http://dx.doi.org/10.7717/peerj.11551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176917PMC
June 2021

Characterization of hydrophobic interaction of galactomannan in aqueous solutions using fluorescence-based technique.

Carbohydr Polym 2021 Sep 8;267:118183. Epub 2021 May 8.

Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China.

Fluorescence probing was used to study hydrophobic interactions of galactomannan (GM) obtained from fenugreek gum (FG), guar gum (GG), and locust bean gum (LBG) at different M/G ratios. The I/I ratio of pyrene changed from 1.73 to 1.29, 1.22, and 1.29 for FG, GG and LBG, respectively, as the concentration of GM increased from 0.01 to 8.0 g/L at 30 °C. The critical aggregation concentration of FG, GG, and LBG increased from 1.04 to 3.84 g/L, 1.15 to 3.73 g/L, and 0.94 to 3.63 g/L, respectively, as temperature increased from 10 to 70 °C. Addition of NaSO and NaSCN increased the I/I ratio in dilute solution, but reduced it in semi-dilute solution, whereas adding urea reduced I/I in dilute solution but increased it in semi-dilute solution. These results indicated that the CAC of GM, polarity and number of hydrophobic microdomains were highly dependent on the M/G ratio and galactose distribution.
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http://dx.doi.org/10.1016/j.carbpol.2021.118183DOI Listing
September 2021

LncRNA PVT1 Facilitates Proliferation, Migration and Invasion of NSCLC Cells via miR-551b/FGFR1 Axis.

Onco Targets Ther 2021 2;14:3555-3565. Epub 2021 Jun 2.

Department of Respiration, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, People's Republic of China.

Background: Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) plays a crucial role in non-small cell lung cancer (NSCLC). Nonetheless, regulatory effects of PVT1 on functions of NSCLC cells remain blurry.

Methods: Relative expression levels of PVT1, miR-551b and FGFR1 mRNA in tumor tissues and cells were examined employing quantitative real-time polymerase chain reaction (qRT-PCR); CCK-8 and BrdU assays were utilized for measuring cell viability and proliferation of H1299 and A549 cells; cell migration and invasion were detected deploying Transwell assay; dual-luciferase assay was used for the validation of binding sequence between PVT1 and miR-551b. FGFR1 expression in protein level was quantified employing Western blot.

Results: PVT1 was highly expressed in NSCLC tissues and cell lines, whereas miR-551b expression was down-regulated. Overexpression of PVT1 potentiated viability, proliferation, migration and invasion of NSCLC cells while miR-551b inhibited the biological behaviors mentioned above. MiR-551b was predicted and then confirmed as a direct downstream target of PVT1. Meanwhile, a negative correlation was observed between PVT1 expression and miR-551b expression in NSCLC tissues. Besides, PVT1 could increase FGFR1 expression by repressing miR-551b expression.

Conclusion: PVT1 promotes the proliferation, migration and invasion of NSCLC cells by indirectly mediating FGFR1 via targeting miR-551b.
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http://dx.doi.org/10.2147/OTT.S273794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180410PMC
June 2021

Thermo-/pH-responsive preservative delivery based on TEMPO cellulose nanofiber/cationic copolymer hydrogel film in fruit packaging.

Int J Biol Macromol 2021 Jul 6;183:1911-1924. Epub 2021 Jun 6.

College of Chemical Engineering, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-forest Biomass, Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China.

Hydrogels have great potential in food packaging. However, stimuli-responsive preservative delivery-based hydrogels for emerging active packaging have not yet been explored. Herein, Unprecedented pH/temperature-responsive hydrogel films for emerging active climacteric fruit packaging were developed based on TEMPO-oxidized nanofibrillated cellulose (TOCNFs) from wheat straw with food-grade cationic-modified poly(N-isopropyl acrylamide-co-acrylamide) (CP). TOCNF incorporation into CP revealed desirable enhancement of characterization, antimicrobial properties, and pH/thermal-responsive behaviour. In-vitro delivery and release mechanism studies with natamycin revealed the fastest release rates in preferred low pH media, up to 32.1 times higher than that under neutral conditions via anomalous diffusion. Applying a thermal stimulus increased natamycin release rates, providing 1.5-21% gradual-additional pulses by Fickian diffusion. The final hydrogel film showed efficient decay control in response to stimuli of the climacteric fruit environment with safe, recyclable, and feasible application demonstrating the significant potential to be used as an alternative-sustainable material for stimuli-triggered preservative delivery in climacteric fruit packaging.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.208DOI Listing
July 2021

Site-specific glycoproteomic analysis revealing increased core-fucosylation on FOLR1 enhances folate uptake capacity of HCC cells to promote EMT.

Theranostics 2021 8;11(14):6905-6921. Epub 2021 May 8.

College of Life Science, Northwest University, Xi'an, Shaanxi province 710069, China.

Epithelial-mesenchymal transition (EMT) has been recognized as an important step toward high invasion and metastasis of many cancers including hepatocellular carcinoma (HCC), while the mechanism for EMT promotion is still ambiguous. The dynamic alterations of site-specific glycosylation during HGF/TGF-β1-induced EMT process of three HCC cell lines were systematically investigated using precision glycoproteomic methods. The possible roles of EMT-related glycoproteins and site-specific glycans were further confirmed by various molecular biological approaches. Using mass spectrometry-based glycoproteomic methods, we totally identified 2306 unique intact glycopeptides from SMMC-7721 and HepG2 cell lines, and found that core-fucosylated glycans were accounted for the largest proportion of complex -glycans. Through quantification analysis of intact glycopeptides, we found that the majority of core-fucosylated intact glycopeptides from folate receptor α (FOLR1) were up-regulated in the three HGF-treated cell lines. Similarly, core-fucosylation of FOLR1 were up-regulated in SMMC-7721 and Hep3B cells with TGF-β1 treatment. Using molecular approaches, we further demonstrated that FUT8 was a driver for HGF/TGF-β1-induced EMT. The silencing of FUT8 reduced core-fucosylation and partially blocked the progress of HGF-induced EMT. Finally, we confirmed that the level of core-fucosylation on FOLR1 especially at the glycosite Asn-201 positively regulated the cellular uptake capacity of folates, and enhanced uptake of folates could promote the EMT of HCC cells. Based on the results, we proposed a potential pathway for HGF or TGF-β1-induced EMT of HCC cells: HGF or TGF-β1 treatment of HCC cells can increase the expression of glycosyltransferase FUT8 to up-regulate the core-fucosylation of -glycans on glycoproteins including the FOLR1; core-fucosylation on FOLR1 can then enhance the folate uptake capacity to finally promote the EMT progress of HCC cells.
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http://dx.doi.org/10.7150/thno.56882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171077PMC
May 2021

Stepwise immobilization of keratin-dopamine conjugates and gold nanoparticles on PET sheets for potential vascular graft with the catalytic generation of nitric oxide.

Colloids Surf B Biointerfaces 2021 Sep 17;205:111855. Epub 2021 May 17.

Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address:

Gold nanoparticles(AuNPs) are capable to catalyze the nitric oxide (NO) generation from endogenous and exogenous donors, thereby promoting re-endothelialization and inhibiting intimal hyperplasia and thrombosis. Herein, keratin-dopamine conjugates were synthesized and then immobilized on the surface of the pre-aminolyzed poly(ethylene terephthalate) (PET) via self-polymerization of dopamine residue, following by the formation of AuNPs in situ without extra reductant. The modified PET sheets(PET-AuNPs) could promote the growth of HUVECs while inhibit the proliferation of HUASMCs due to their catalytic generation of NO from GSNO. In addition, these sheets exhibited antibacterial properties and good blood compatibility without hemolysis. Taken together, this strategy for designing prosthetic vascular grafts to treat cardiovascular diseases has great potential.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111855DOI Listing
September 2021

Diffusion of water and protein drug in 1,4-butanediol diglycidyl ether crosslinked galactomannan hydrogels and its correlation with the physicochemical properties.

Int J Biol Macromol 2021 Jul 2;183:1987-2000. Epub 2021 Jun 2.

Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China. Electronic address:

The aim of the present study was to obtain a better and safer galactomannan-based material for drug release applications. A novel epoxy-crosslinked galactomannan hydrogel (EGH) was prepared from guar gum using 1,4-butanediol diglycidyl ether as a crosslinking agent. The diffusion rate constant of water molecules in freeze-dried EGH positively correlated with water uptake/equilibrium swelling rate (WU/ESR), and the water molecules participated in Fickian diffusion. The ESR, WU/ESR, and bovine serum albumin (BSA) loading capacity of a customized EGH with a crosslinking density of 48.9% were 48.7 ± 0.15 g/g, 95.3%, and 56.4 mg/g, respectively. The release of BSA from freeze-dried EGH was affected by the WU/ESR and the pH; the release equilibrium time was ~40 h at pH 1.2, decreasing to ~24 h at pH 7.4. Furthermore, the cumulative release rate increased from 63.5% to 80.7% and the t decreased from 59 to 41 min upon changing from the acidic to basic pH. The release process conformed to the Ritger-Peppas and Hixson-Crowell models, and represented Fickian diffusion and chain relaxation. The EGH showed no cytotoxicity toward HeLa cells. Together, these results demonstrate the properties of a novel galactomannan-based hydrogel that can potentially be employed as a vehicle for drug delivery.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.195DOI Listing
July 2021

Feasibility of perfusion and early-uptake F-FDG PET/CT in primary hepatocellular carcinoma: a dual-input dual-compartment uptake model.

Jpn J Radiol 2021 Jun 2. Epub 2021 Jun 2.

Faculty of Information Engineering and Automation, Kunming University of Science and Technology, 727 South Jingming Rd., Kunming, Yunnan, China.

Purpose: PET enables a concurrent evaluation of perfusion status and metabolic activity. We aimed to evaluate the feasibility of perfusion and early-uptake F-FDG PET/CT in hepatocellular carcinoma (HCC) using a dual-input dual-compartment uptake model.

Materials And Methods: Data from 5 min dynamic PET/CT and conventional PET/CT scans were retrospectively collected from 17 pathologically diagnosed HCCs. Parameters such as hepatic arterial blood flow (Fa), portal vein blood flow (Fv), total blood flow (F), hepatic arterial perfusion index (HPI), portal vein perfusion index (PPI), blood volume (BV), extracellular mean transit time (MTT) and intracellular uptake rate (Ki) were calculated. Fa, HPI, MTT and Ki images were generated and used to identify HCC.

Results: Compared with the surrounding liver tissue, HCCs showed significant increases in Fa, HPI, Ki and the maximum standard uptake value (SUVmax) (all P < 0.001) and significant reductions in Fv (P < 0.05) and PPI (P < 0.001). F, BV and MTT (all P > 0.05) did not differ significantly between HCCs and the surrounding liver tissue. Perfusion and early-uptake PET/CT increased the positivity rate of HCCs from 52.9% with conventional PET/CT alone to 88.2% with the combined method (P < 0.05).

Conclusions: Perfusion and early-uptake PET/CT are feasible for diagnosing HCC and provide added functional information to enhance diagnostic performance.
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http://dx.doi.org/10.1007/s11604-021-01140-6DOI Listing
June 2021

Clinical efficacy and adverse reactions of transforaminal interbody fusion combined with pedicle screw fixation in the treatment of lumbar spondylolisthesis.

Minerva Med 2021 May 31. Epub 2021 May 31.

Department of Orthopaedics, Harrison International Peace Hospital of Hebei Medical University, Hengshui, China -

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http://dx.doi.org/10.23736/S0026-4806.21.07514-5DOI Listing
May 2021

Identification of Hub Genes to Regulate Breast Cancer Spinal Metastases by Bioinformatics Analyses.

Comput Math Methods Med 2021 12;2021:5548918. Epub 2021 May 12.

Department of Pathology, Inner Mongolia People's Hospital, Hohhot, 010017 Inner Mongolia, China.

Breast cancer (BC) had been one of the deadliest types of cancers in women worldwide. More than 65% of advanced-stage BC patients were identified to have bone metastasis. However, the molecular mechanisms involved in the BC spinal metastases remained largely unclear. This study screened dysregulated genes in the progression of BC spinal metastases by analyzing GSE22358. Moreover, we constructed PPI networks to identify key regulators in this progression. Bioinformatics analysis showed that these key regulators were involved in regulating the metabolic process, cell proliferation, Toll-like receptor and RIG-I-like receptor signaling, and mRNA surveillance. Furthermore, our analysis revealed that key regulators, including C1QB, CEP55, HIST1H2BO, IFI6, KIAA0101, PBK, SPAG5, SPP1, DCN, FZD7, KRT5, and TGFBR3, were correlated to the OS time in BC patients. In addition, we analyzed TCGA database to further confirm the expression levels of these hub genes in breast cancer. Our results showed that these regulators were significantly differentially expressed in breast cancer, which were consistent with GSE22358 dataset analysis. Furthermore, our analysis demonstrated that CEP55 was remarkably upregulated in the advanced stage of breast cancer compared to the stage I breast cancer sample and was significantly upregulated in triple-negative breast cancers (TNBC) compared to other types of breast cancers, including luminal and HER2-positive cancers, demonstrating CEP55 may have a regulatory role in TNBC. Finally, our results showed that CEP55 was the most highly expressed in Basal-like 1 TNBC and Basal-like 2 TNBC samples but the most lowly expressed in mesenchymal stem-like TNBC samples. Although more studies are still needed to understand the functions of key regulators in BC, this study provides useful information to understand the mechanisms underlying BC spinal metastases.
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http://dx.doi.org/10.1155/2021/5548918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133842PMC
May 2021

Magnesium Fertilization Affected Rice Yields in Magnesium Sufficient Soil in Heilongjiang Province, Northeast China.

Front Plant Sci 2021 11;12:645806. Epub 2021 May 11.

College of Resources and Environment, Northeast Agricultural University, Harbin, China.

Magnesium (Mg) plays a crucial role in rice yield. Heilongjiang Province is the main rice-producing region of China, playing an important role in guaranteeing China's and the world's grain security. However, rarely Mg fertilization is applied in this province. Soil Mg status of main rice-producing areas in Heilongjiang Province was investigated and Mg fertilizer experiments were conducted aiming to provide fertilizer recommendation in this region. A total of 358 soil samples from the 0-20 cm and 20-40 cm soil layer from the main rice-producing areas of Heilongjiang Province were collected to analyze soil exchangeable Mg (ex-Mg) and relative chemical properties. Meanwhile, field experiments of soil and foliar Mg application were performed in 2017-2019 to identify the effect of this nutrient on rice yield. The results showed that the ex-Mg concentration in the 0-20 cm and 20-40 cm soil layer was 282 mg kg and 243 mg kg, respectively. Moreover, ex-Mg ranged on the abundant and exceptionally abundant level accounted for 75% in 0-20 cm and 55.3% in 20-40 cm. The ex-Mg concentration in the upper soil layer was higher than in the lower soil layer and varied depending on regions, which the west part of Heilongjiang Province showed the highest concentration in both soil layers. Correlation analysis showed that there had a significant ( < 0.05) linear relationship between ex-Mg and pH, CEC, ex-K, Ca, K/Mg, and Ca/Mg. Meanwhile, the results of path coefficients demonstrated that pH, CEC, and Ca/Mg had the most direct effect on ex-Mg concentration among these above factors. Soil Mg application had little effect on rice yield, which might be related to the soil Mg concentration and availability, and root uptake activity. Foliar Mg application increased rice yield by 8.45% ( < 0.05) compared to without Mg treatment, increased 1,000-grain weight by 2.62% ( < 0.05), and spikelet number per panicle by 4.19% ( < 0.05). In general, the paddy soil ex-Mg concentration in Heilongjiang Province was abundant. Soil-applied Mg played little role in rice yield in ex-Mg abundant regions, while foliar application increased rice yields significantly via increasing 1,000-grain weight and spikelet number per panicle.
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http://dx.doi.org/10.3389/fpls.2021.645806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144475PMC
May 2021

Pelvic floor function and morphological abnormalities in primiparas with postpartum symptomatic stress urinary incontinence based on the type of delivery: a 1:1 matched case-control study.

Int Urogynecol J 2021 May 24. Epub 2021 May 24.

Obstetrics and Gynecology Department, Tianjin Hospital, Tianjin, China.

Introduction And Hypothesis: The aim of this study was to assess pelvic floor muscle (PFM) morphology and function in primiparas with postpartum symptomatic SUI after different types of delivery.

Methods: Retrospective analyses were carried out with individuals with postpartum symptomatic stress urinary incontinence (SUI). Among the women screened in our center from January 2018 to December 2019, participants were divided into elective cesarean section (eCS) and spontaneous vaginal delivery (sVD) groups, while being matched 1:1 on age (±5 years), body mass index (BMI; ±0.5 kg/m), neonatal birth weight (±300 g), gestational age (±1 week), degree of pelvic organ prolapse quantification (POP-Q), International Consultation on Incontinence Questionnaire-Urinary Incontinence Short Form (ICIQ-UI SF) degree, Incontinence Impact Questionnaire short form (IIQ-7) score, and postpartum days (±10 days); all participants had no sphincter defects or levator ani muscle avulsion. The bioelectrical activity of the PFM was collected using an endovaginal electrode with the Glazer protocol. For the assessment of PFM function, PFM morphometry was evaluated with 3D/4D transperineal ultrasound.

Results: A total of 78 matched pairs were recruited based on delivery mode. Regarding functional differences, both fast-twitch and slow-twitch fiber strengths in the eCS group were significantly higher than those in the sVD group, but PFMs were more hyperactive in the eCS group. Regarding morphometric differences, the retrovesical angle (RVA) and bladder neck position were not significantly different in the resting state between the two groups, nor was the RVA during the Valsalva maneuver (eCS group: 130.68 ± 17.08°, sVD group: 136.33 ± 23.93°), p > 0.05. There were differences in bladder neck descent (BND; eCS group: 16.51 ± 7.55 mm, sVD group: 23.92 ± 8.47 mm) and urethral rotation angle (URA; eCS group: 37.53 ± 26.05°, sVD group: 59.94 ± 25.87°), all p < 0.05. BND showed a negative correlation with PFM strength, p < 0.05. URAs and RVAs showed no correlation with PFM strength, p > 0.05.

Conclusion: Pelvic floor muscle function disorder, hyperactivity, and instability also occurred after eCS, which resulted in postpartum symptomatic SUI. The effects of sVD compared with eCS on abnormalities in the lower urinary tract were related to bladder neck and urethral hyperactivity, without an RVA increase.
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http://dx.doi.org/10.1007/s00192-021-04816-9DOI Listing
May 2021

Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1.

Arch Virol 2021 Jul 14;166(7):2005-2010. Epub 2021 May 14.

Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center, Room Na-1005, Wytemaweg 80, 3015 CN, Rotterdam, The Netherlands.

We show that ivermectin, an FDA-approved anti-parasitic drug, effectively inhibits infection with hepatitis E virus (HEV) genotypes 1 and 3 in a range of cell culture models, including hepatic and extrahepatic cells. Long-term treatment showed no clear evidence of the development of drug resistance. Gene silencing of importin-α1, a cellular target of ivermectin and a key member of the host nuclear transport complex, inhibited viral replication and largely abolished the anti-HEV effect of ivermectin.
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http://dx.doi.org/10.1007/s00705-021-05096-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119233PMC
July 2021
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