Publications by authors named "Pengfei Zhang"

743 Publications

Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress.

Oxid Med Cell Longev 2021 15;2021:7848027. Epub 2021 Apr 15.

Department of Cardiology, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, China.

Abnormal autophagy and oxidative stress contribute to angiotensin II- (Ang II-) induced cardiac hypertrophy and heart failure. We previously showed that Ang II increased Rap1GAP gene expression in cardiomyocytes associated with hypertrophy and autophagy disorders. Using real-time PCR and Western blot, we found that Rap1GAP expression was increased in the heart of Sprague Dawley (SD) rats infused by Ang II compared with saline infusion and in Ang II vs. vehicle-treated rat neonatal cardiomyocytes. Overexpression of Rap1GAP in cultured cardiomyocytes exacerbated Ang II-induced cardiomyocyte hypertrophy, reactive oxygen species (ROS) generation, and cell apoptosis and inhibited autophagy. The increased oxidative stress caused by Rap1GAP overexpression was inhibited by the treatment of autophagy agonists. Knockdown of Rap1GAP by siRNA markedly attenuated Ang II-induced cardiomyocyte hypertrophy and oxidative stress and enhanced autophagy. The AMPK/AKT/mTOR signaling pathway was inhibited by overexpression of Rap1GAP and activated by the knockdown of Rap1GAP. These results show that Rap1GAP-mediated pathway might be a new mechanism of Ang II-induced cardiomyocyte hypertrophy, which could be a potential target for the future treatment of cardiac hypertrophy and heart failure.
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http://dx.doi.org/10.1155/2021/7848027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062190PMC
May 2021

Presence of voids after three obturation techniques in band-shaped isthmuses: a micro-computed tomography study.

BMC Oral Health 2021 05 1;21(1):227. Epub 2021 May 1.

Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011, China.

Background: The objective of the present micro-computed tomography (micro-CT) study was to assess the presence of voids in band-shaped isthmuses obturated using three different filling techniques.

Methods: Twenty-four artificial molar teeth with a band-shaped isthmus were allocated to three groups (n = 8) for obturation, according to the filling technique: single-cone (SC), continuous wave of condensation (CWC) or lateral condensation (LC). Obturation was performed with gutta-percha (GP) cones and iRoot SP (Innovative Bioceramix, Vancouver, Canada). Post-filling micro-CT scanning was performed. The percentage of filling materials and void volumes were calculated in the isthmus areas and data were analyzed using one-way ANOVA and Tukey tests.

Results: The mean percentage of void volumes and corresponding filling percentages in the isthmus areas after obturation in the SC groups was 22.98 % ± 1.19 %, 77.02 % ± 1.19 %; in the CWC groups 10.46 % ± 2.28 %, 89.54 % ± 2.28 %; and in the LC groups was 13.14 % ± 1.85 %, 86.86 % ± 1.85 %, respectively.

Conclusions: In band-shaped isthmus area, the obturation quality of CWC was superior to SC and LC techniques.
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http://dx.doi.org/10.1186/s12903-021-01584-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088625PMC
May 2021

Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets ( L.).

Plants (Basel) 2021 Apr 28;10(5). Epub 2021 Apr 28.

College of Agriculture, Northeast Agricultural University, Harbin 150030, China.

Melatonin has been regarded as a promising substance that enhances the abiotic stress tolerance of plants. However, few studies have devoted attention to the role of melatonin in improving salt tolerance in sugar beets. Here, the effects of different application methods (foliar application (100 μM), root application (100 μM), and combined foliar and root application) of melatonin on the morphological and physiological traits of sugar beets exposed to salt stress were investigated. The results showed that melatonin improved the growth of sugar beet seedlings, root yield and sugar content, synthesis of chlorophyll, photosystem II (PS II) activity, and gas exchange parameters under salt stress conditions. Moreover, melatonin enhanced the capacity of osmotic adjustment by increasing the accumulation of osmolytes (betaine, proline, and soluble sugar). At the same time, melatonin increased the H-pump activities in the roots, thus promoting Na efflux and K influx, which maintained K/Na homeostasis and mitigated Na toxicity. In addition, melatonin strengthened the antioxidant defense system by enhancing the activities of antioxidant enzymes, modulating the ASA-GSH cycle, and mediating the phenylalanine pathway, which removed superoxide anions (O) and hydrogen peroxide (HO) and maintained cell membrane integrity. These positive effects were more pronounced when melatonin was applied by combined foliar and root application. To summarize, this study clarifies the potential roles of melatonin in mitigating salt stress in sugar beets by improving photosynthesis, water status, ion homeostasis, and the antioxidant defense system.
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http://dx.doi.org/10.3390/plants10050886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146524PMC
April 2021

Potential risk analysis and experience summarization of unstable factors of cranial fixation devices in neurosurgical operations: three-case reports and systematic review.

Chin Neurosurg J 2021 Apr 28;7(1):25. Epub 2021 Apr 28.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: The use of cranial fixation devices in neurosurgery is very common, which is considered to be an important auxiliary method for many craniotomies. However, previous studies have reported complications of using cranial fixation devices, including brain tissue, nerve and blood vessel damage, scalp laceration, subcutaneous hematoma, etc. Some of the complications are serious and even potentially fatal, and the causes of which may be related to the incorrect use of cranial fixation devices. Although there are no serious complications in our review, the cause of that needs to be further summarized and analyzed, as so to minimize the serious consequences caused by the cranial fixation device slippage and ensure the safety of the patients' surgical procedure.

Case Presentation: In our recent work, we have continuously found three cases of unstable cranial fixation devices, which make us to analyze the possible factors and summarize experience combined with the review of other senior neurosurgeons (more than 3 years of working experience) from different departments of neurosurgery.

Conclusions: Based on our recent incidents of unstable cranial fixation and the experience of investigating and analyzing senior doctors from different neurosurgery centers, we summarized experience to minimize the risk of unstable cranial fixation. We tried a variety of options, including a safe anatomical location for cranial fixation, teamwork, and communication with anesthesiologists and itinerant nurses, to ensure the stability of the patient's cranial fixation devices. The data obtained in this survey has great limitations, including the doctor's personal prejudice and dependence on anecdotal memories. Therefore, the data should be interpreted with caution. However, there are still some modes that can help to better understand the use of safe cranial fixation. Based on the above research and analysis, we have made recommendations that may help neurosurgeons to avoid preventable complications.
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http://dx.doi.org/10.1186/s41016-021-00244-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080414PMC
April 2021

Facile syringe filter-enabled bacteria separation, enrichment, and buffer exchange for clinical isolation-free digital detection and characterization of bacterial pathogens in urine.

Analyst 2021 Apr;146(8):2475-2483

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA. and Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.

The development of accelerated methods for pathogen identification (ID) and antimicrobial susceptibility testing (AST) for infectious diseases is necessary to facilitate evidence-based antibiotic therapy and reduce clinical overreliance on broad-spectrum antibiotics. Towards this end, droplet-based microfluidics has unlocked remarkably rapid diagnostic assays with single-cell and single-molecule resolution. Yet, droplet platforms invariably rely on testing purified bacterial samples that have been clinically isolated after lengthy (>16 h) plating. While plating-based clinical isolation is important for enriching and separating out bacteria from background in clinical samples and also facilitating buffer exchange, it creates a diagnostic bottleneck that ultimately precludes droplet-based methods from achieving significantly accelerated times-to-result. To alleviate this bottleneck, we have developed facile syringe filter-enabled strategies for bacterial separation, enrichment, and buffer exchange from urine samples. By selecting appropriately sized filter membranes, we separated bacterial cells from background particulates in urine samples and achieved up to 91% bacterial recovery after such 1-step filtration. When interfaced with droplet-based detection of bacterial cells, 1-step filtration improved the limit of detection for bacterial ID and quantification by over an order of magnitude. We also developed a facile buffer exchange strategy to prepare bacteria in urine samples for droplet-based AST that achieved up to 10-fold bacterial enrichment during buffer exchange. Our filtration strategies, can be easily integrated into droplet workflows, enable clinical isolation-free sample-to-answer ID and AST, and significantly accelerate the turnaround of standard infectious disease diagnostic workflows.
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http://dx.doi.org/10.1039/d1an00039jDOI Listing
April 2021

Identification of NTRK3 as a potential prognostic biomarker associated with tumor mutation burden and immune infiltration in bladder cancer.

BMC Cancer 2021 Apr 24;21(1):458. Epub 2021 Apr 24.

Department of Urology, The Affiliated Hospital of Qingdao University, No.16 Jiangsu Road, Qingdao, 266000, China.

Background: Bladder cancer (BLCA) is a common malignant tumor of urinary system with high morbidity and mortality. In recent years, immunotherapy has played a significant role in the treatment of BLCA. Tumor mutation burden (TMB) has been reported to be a powerful biomarker for predicting tumor prognosis and efficacy of immunotherapy. Our study aimed to explore the relationship between TMB, prognosis and immune infiltration to identify the key genes in BLCA.

Methods: Clinical information, somatic mutation and gene expression data of BLCA patients were downloaded from The Cancer Genome Atlas (TCGA) database. Patients were divided into high and low TMB groups according to their calculated TMB scores. Gene Set Enrichment Analysis (GSEA) was performed to screen for significantly enriched pathways. Differentially expressed genes (DEGs) between the two groups were identified. Univariate Cox analysis and Kaplan-Meier survival analysis were applied for screening key genes. Immune infiltration was performed for TMB groups and NTRK3.

Results: Higher TMB scores were related with poor survival in BLCA. After filtering, 36 DEGs were identified. NTRK3 had the highest hazard ratio and significant prognostic value. Co-expressed genes of NTRK3 were mainly involved in several pathways, including DNA replication, basal transcription factors, complement and coagulation cascades, and ribosome biogenesis in eukaryotes. There was a significant correlation among TMB scores, NTRK3 expression and immune infiltration.

Conclusions: Our results suggest that NTRK3 is a TMB-related prognostic biomarker, which lays the foundation for further research on the immunomodulatory effect of NTRK3 in BLCA.
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http://dx.doi.org/10.1186/s12885-021-08229-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070296PMC
April 2021

Immunoengineered magnetic-quantum dot nanobead system for the isolation and detection of circulating tumor cells.

J Nanobiotechnology 2021 Apr 23;19(1):116. Epub 2021 Apr 23.

Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.

Background: Highly efficient capture and detection of circulating tumor cells (CTCs) remain elusive mainly because of their extremely low concentration in patients' peripheral blood.

Methods: We present an approach for the simultaneous capturing, isolation, and detection of CTCs using an immuno-fluorescent magnetic nanobead system (iFMNS) coated with a monoclonal anti-EpCAM antibody.

Results: The developed antibody nanobead system allows magnetic isolation and fluorescent-based quantification of CTCs. The expression of EpCAM on the surface of captured CTCs could be directly visualized without additional immune-fluorescent labeling. Our approach is shown to result in a 70-95% capture efficiency of CTCs, and 95% of the captured cells remain viable. Using our approach, the isolated cells could be directly used for culture, reverse transcription-polymerase chain reaction (RT-PCR), and immunocytochemistry (ICC) identification. We applied iFMNS for testing CTCs in peripheral blood samples from a lung cancer patient.

Conclusions: It is suggested that our iFMNS approach would be a promising tool for CTCs enrichment and detection in one step.
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http://dx.doi.org/10.1186/s12951-021-00860-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063296PMC
April 2021

Noiseless photonic non-reciprocity via optically-induced magnetization.

Nat Commun 2021 Apr 22;12(1):2389. Epub 2021 Apr 22.

CAS Key Laboratory of Quantum Information & CAS Center For Excellence in Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei, P. R. China.

The realization of optical non-reciprocity is crucial for many applications, and also of fundamental importance for manipulating and protecting the photons with desired time-reversal symmetry. Recently, various new mechanisms of magnetic-free non-reciprocity have been proposed and implemented, avoiding the limitation of the strong magnetic field imposed by the Faraday effect. However, due to the difficulties in separating the signal photons from the drive laser and the noise photons induced by the drive laser, these devices exhibit limited isolation performances and their quantum noise properties are rarely studied. Here, we demonstrate an approach of magnetic-free non-reciprocity by optically-induced magnetization in an atom ensemble. Excellent isolation (highest isolation ratio is [Formula: see text]) is observed over a power dynamic range of 7 orders of magnitude, with the noiseless property verified by quantum statistics measurements. The approach is applicable to other atoms and atom-like emitters, paving the way for future studies of integrated photonic non-reciprocal devices.
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http://dx.doi.org/10.1038/s41467-021-22597-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062452PMC
April 2021

Hypervalent iodine(iii)-promoted rapid cascade reaction for the synthesis of unsymmetric azo compounds.

Org Biomol Chem 2021 Apr 23;19(14):3119-3123. Epub 2021 Mar 23.

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, China.

A rapid three-component cascade reaction for the synthesis of unsymmetric azo compounds via a radical activation strategy has been reported. Various aryldiazonium salts and unactivated alkenes are well compatible, providing the corresponding products in good to excellent yields. This strategy gives an efficient and practical solution for the synthesis of unsymmetric azo compounds with two C-N bond formation. A free radical pathway mechanism is advised for this transformation.
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http://dx.doi.org/10.1039/d1ob00219hDOI Listing
April 2021

Simulation analysis of implementation effects of construction and demolition waste disposal policies.

Waste Manag 2021 May 17;126:684-693. Epub 2021 Apr 17.

China Railway 14TH Bureau Group Co., Ltd, PR China.

Urban development generates large amounts of construction and demolition waste, and the enactment of various policies is an important way to increase resource efficiency. It is urgent to analyse the choices and effects of construction and demolition waste disposal policies around the world. This study analyses and compares the implementation effects of various construction and demolition waste disposal policies and provides suggestions on the selection and formulation of different disposal method policies. A BP neural network and an agent-based model are used to construct a simulation model for the implementation effects of construction and demolition waste disposal policies. The average acceptance willingness is analysed for the three disposal methods of reduction, reuse and recycling under the different policies based on questionnaire survey data. Simulation results show that the implementation effect of the incentive policy as a single policy is superior, and the comprehensive implementation effect of the guidance-incentive-mandatory policy as a combined policy is superior. The implementation effect of combined policies is better than that of single policies. The policies suitable for different disposal methods are inconsistent. Information interaction can effectively improve the implementation effects of different construction and demolition waste disposal policies, especially for single policies. This study innovatively compares the various construction and demolition waste disposal policies and explain the interaction during their implementation. The findings can help the government formulate policies at different stages of construction and demolition waste management and provide a new approach to policy simulation research.
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http://dx.doi.org/10.1016/j.wasman.2021.03.056DOI Listing
May 2021

Peripapillary Vessel Density in Eyes with Rhegmatogenous Retinal Detachment after Pars Plana Vitrectomy.

J Ophthalmol 2021 23;2021:6621820. Epub 2021 Mar 23.

Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Purpose: To investigate the vascular density of the optic nerve head (ONH) and macula using optical coherence tomography angiography (OCTA) in patients undergoing vitrectomy for rhegmatogenous retinal detachment (RRD) and to evaluate associations with visual outcomes.

Methods: Patients with RRD, successfully treated with a pars plana vitrectomy (PPV) and a minimum three-month follow-up were included in this study. The vessel density (VD) of the ONH and peripapillary regions, foveal avascular zone (FAZ), foveal flow density (FFD), and parafoveal flow density (PFD) were evaluated using OCTA and compared to the fellow eye. Multivariate linear regression analysis was performed to determine correlations with visual outcomes.

Results: Thirty-one patients with macula-off RRD were included in the study. Compared with the fellow eyes, eyes after RRD surgery had a lower peripapillary VD ( < 0.01). No significant difference in superficial and deep FFD, PFD, and FAZ area was found compared to the fellow eyes. Postoperative peripapillary VD and baseline BCVA were significantly associated with BCVA three months after PPV ( < 0.05).

Conclusion: Rhegmatogenous retinal detachment eyes successfully treated with PPV had lower peripapillary vessel density than fellow healthy eyes. Postoperative BCVA was related to postoperative peripapillary VD.
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http://dx.doi.org/10.1155/2021/6621820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009721PMC
March 2021

High-efficiency coupling of single quantum emitters into hole-tailored nanofibers.

Opt Express 2021 Mar;29(7):11158-11168

We propose a scheme to enhance the coupling efficiency of photons from a single quantum emitter into a hole-tailored nanofiber. The single quantum emitter is positioned inside a circular hole etched along the radial axis of the nanofiber. The coupling efficiency can be effectively enhanced and is twice as high as the case in which only an intact nanofiber without the hole is used. The effective enhancement independent of a cavity can avoid the selection of a single emitter for the specific wavelength, which means a broad operating wavelength range. Numerical simulations are performed to optimize the coupling efficiency by setting appropriate diameters of the nanofiber and the hole. The simulation results show that the coupling efficiency can reach 62.8% when the single quantum emitter with azimuthal polarization (x direction) is at a position 200 nm from the middle of the hole along the hole-axial direction. The diameters of the nanofiber and the hole are 800 nm and 400 nm, respectively, while the wavelength of the single quantum emitter is 852 nm. Hole-tailored nanofibers have a simple configuration and are easy to fabricate and integrate with other micro/nanophotonic structures; this fiber structure has wide application prospects in quantum information processing and quantum precision measurement.
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http://dx.doi.org/10.1364/OE.420243DOI Listing
March 2021

Revealing New Candidate Genes for Teat Number Relevant Traits in Duroc Pigs Using Genome-Wide Association Studies.

Animals (Basel) 2021 Mar 13;11(3). Epub 2021 Mar 13.

Key Laborary of Animal Genetics, Breeding and Reproduction (Poultry) of Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The number of teats is related to the nursing ability of sows. In the present study, we conducted genome-wide association studies (GWAS) for traits related to teat number in Duroc pig population. Two mixed models, one for counted and another for binary phenotypic traits, were employed to analyze seven traits: the right (RTN), left (LTN), and total (TTN) teat numbers; maximum teat number on a side (MAX); left minus right side teat number (LR); the absolute value of LR (ALR); and the presence of symmetry between left and right teat numbers (SLR). We identified 11, 1, 4, 13, and 9 significant SNPs associated with traits RTN, LTN, MAX, TTN, and SLR, respectively. One significant SNP (MARC0038565) was found to be simultaneous associated with RTN, LTN, MAX and TTN. Two annotated genes ( and 1) were located in genomic region around this SNP. Three significant SNPs were shown to be associated with TTN, RTN and MAX traits. Seven significant SNPs were simultaneously detected in two traits of TTN and RTN. Other two SNPs were only identified in TTN. These 13 SNPs were clustered in the genomic region between 96.10-98.09 Mb on chromosome 7. Moreover, nine significant SNPs were shown to be significantly associated with SLR. In total, four and 22 SNPs surpassed genome-wide significance and suggestive significance levels, respectively. Among candidate genes annotated, eight genes have documented association with the teat number relevant traits. Out of them, genes on chromosome (SSC) 7 and the gene on SSC 10 were new candidate genes identified in this study. Our findings demonstrate the genetic mechanism of teat number relevant traits and provide a reference to further improve reproductive performances in practical pig breeding programs.
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http://dx.doi.org/10.3390/ani11030806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998181PMC
March 2021

AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B and cyclopiazonic acid as an example.

Biosens Bioelectron 2021 Jun 20;182:113188. Epub 2021 Mar 20.

National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. Electronic address:

Food safety is currently a significant issue for human life and health. Various fluorescent nanomaterials have been applied in the point-of-care test (POCT) for food safety as labeling materials. However, previous fluorescent nanomaterials can cause aggregation-caused quenching (ACQ), thus reducing the detection sensitivity. Conversely, aggregation-induced emission luminogens (AIEgens) are promising candidates for POCT in the food safety field because they can enhance detection sensitivity and throughput. Mycotoxins, such as aflatoxin B (AFB) and cyclopiazonic acid (CPA), are a primary threat to human life and health and a significant food safety issue, and their on-site detection from farm to table is needed. Herein, an ultrasensitive point-of-care test was developed based on TPE-Br, a blue-emissive tetraphenylethylene derivative AIEgen. Under optimal conditions, this AIEgen-based lateral-flow biosensor (ALFB) allowed for a rapid response of 8 min toward AFB and CPA detection, with considerable sensitivities of 0.003 and 0.01 ng/mL in peanut matrices, respectively. In peanut matrices, the recoveries were 90.3%-110.0% for both mycotoxins, with relative standard deviations (RSDs) below 6%. The ALFB was further validated via UPLC-MS/MS using spiked peanut samples. AIEgens open an avenue for on-site, ultrasensitive, high-throughput detection methods and can be extensively used in point-of-care tests in food safety.
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http://dx.doi.org/10.1016/j.bios.2021.113188DOI Listing
June 2021

Does functional specialization affect collaborative development? Evidence from four Chinese metropolitan areas.

Environ Sci Pollut Res Int 2021 Mar 31. Epub 2021 Mar 31.

Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China.

The layout of urban spatial functions may play an important role in sustainable regional development. Given the rapid development of urbanization in China, this study attempts to analyze how functional specialization affects coordinated regional development. To quantitatively measure the degree of coordinated development within different regions, the coordinated degree index is used to calculate the degree of coordinated development of four Chinese metropolitan areas from 2008 to 2019. The panel vector autoregressive (PVAR) model is employed to empirically test the impact of urban functional specialization on coordinated regional development. The results indicate that high market activity is conducive to the formation of functional specialization synergy in space, and the level of social productivity directly affects the functional specialization and the level of synergy within a region. With the continuous strengthening of the implementation of local government policies, the functional specialization and synergy in metropolitan areas have gradually strengthened. However, the promotion effects and the expression of functional specialization, the coordinated development of government actions, and spatial functions differ across metropolitan areas in China.
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http://dx.doi.org/10.1007/s11356-021-13568-4DOI Listing
March 2021

The effects of feeding finishing pigs of two genders with a high fiber and high fat diet on muscle glycolytic potential at slaughter and meat quality.

Meat Sci 2021 Jul 27;177:108484. Epub 2021 Feb 27.

Agriculture and Agri-Food Canada, Sherbrooke Research and Development Centre, Sherbrooke, QC J1M 0C8, Canada. Electronic address:

A total of 160 pigs, in groups of 8 pigs of mixed genders, were fed four finishing feeding strategies with the aim to reduce muscle glycolytic potential and improve meat quality. Pigs were fed a control diet (C; fat = 5.0%, ADF = 3.0%, NDF = 8.8%), a high-fat and high-fiber diet (HFF; fat = 11.2%, ADF = 9.1%, NDF = 19.5%), a blend of 50-50% C and HFF diets (fat = 8.2%, ADF = 6.7%, NDF = 14.2%) or the C diet and transferred to the HFF diet after a diet transition. Dietary treatments alone or in interaction with gender had no effect on pig growth performance, carcass quality traits, Longissimus and Semimembranosus muscle glycolytic potential and meat quality (P > 0.10). The inefficiency of the dietary treatments applied in this study may be due to the low ratio between fat and digestible carbohydrate in the diets combined with the mild pre-slaughter stress conditions pigs were exposed to.
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http://dx.doi.org/10.1016/j.meatsci.2021.108484DOI Listing
July 2021

Overcoming the phase separation within high-entropy metal carbide by poly(ionic liquid)s.

Chem Commun (Camb) 2021 Apr 16;57(30):3676-3679. Epub 2021 Mar 16.

The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China.

High-entropy crystalline materials are attracting more attention. In principle, high-entropy metal carbides (HMCs) that contain five or more metal ions, possess more negative free energy value during catalysis. But its preparation is challenging because of the immiscibility of multi metal cations in a single carbide solid solution. Here, a rational strategy for preparing HMC is proposed via a coordination-assisted crystallization process in the presence of Br-based poly(ionic liquids). Through this method, MoWVCrNbC nanoparticles, with a single cubic phase structure, incorporated on porous carbon, are obtained ([email protected]). By combination of well dispersed small particle size (∼4 nm), high surface area (∼270 m g), and high-entropy phase, [email protected] can function as a promising catalyst for the dehydrogenation of ethylbenzene. Unexpected activity (EB conv.: 73%) and thermal stability (>100 h on steam) at 450 °C are observed. Such a facile synthetic strategy may inspire the fabrication of other types of HMCs for more specific tasks.
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http://dx.doi.org/10.1039/d1cc00497bDOI Listing
April 2021

Quantification of Single-Molecule Protein Binding Kinetics in Complex Media with Prism-Coupled Plasmonic Scattering Imaging.

ACS Sens 2021 03 15;6(3):1357-1366. Epub 2021 Mar 15.

Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, Arizona 85287 United States.

Measuring molecular binding is critical for understanding molecular-scale biological processes and screening drugs. Label-free detection technologies, such as surface plasmon resonance (SPR), have been developed for analyzing analytes in their natural forms. However, the specificity of these methods is solely relying on surface chemistry and has often nonspecific binding issues when working with samples in complex media. Herein, we show that single-molecule-based measurement can distinct specific and nonspecific binding processes by quantifying the mass and binding dynamics of individual-bound analyte molecules, thus allowing the binding kinetic analysis in complex media such as serum. In addition, this single-molecule imaging is realized in a commonly used Kretschmann prism-coupled SPR system, thus providing a convenient solution to realize high-resolution imaging on widely used prism-coupled SPR systems.
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http://dx.doi.org/10.1021/acssensors.0c02729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046548PMC
March 2021

Non-interacting, Non-opioid, and Non-barbiturate Containing Acute Medication Combinations in Headache: A Pilot Combinatorics Approach Based on DrugBank Database.

Front Neurol 2021 17;12:632830. Epub 2021 Feb 17.

Department of Neurology, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ, United States.

Polypharmacy in abortive medications is often inevitable for patients with refractory headaches. We seek to enumerate an exhaustive list of headaches abortive medications that are without drug-drug interactions. We updated a list of acute medications based on the widely used Jefferson Headache Manual with novel abortive medications including ubrogepant, lasmiditan, and rimegepant. Opioids and barbiturate-containing products are excluded. From this resultant list of medications, we then conducted an exhaustive search of all pair-wise interactions via DrugBank's API. Using this interaction list, we filtered all possible two, three, and four drug combinations of abortive medications. The list of medications was then reapplied to DrugBank to verify the lack of known drug-drug interactions. There are 192 medication combinations that do not contain any drug-drug interactions. Most common elements in these combinations are ubrogepant, prochlorperazine, followed by tizanidine. There are 67 three-drug combinations that do not contain interactions. Only two of the four-drug combinations do not yield some form of drug-drug interactions. This list of headaches abortive medications without drug-drug interactions is a useful tool for clinicians seeking to more effectively manage refractory headaches by implementing a rational polypharmacy.
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http://dx.doi.org/10.3389/fneur.2021.632830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925628PMC
February 2021

ECG-based biometric under different psychological stress states.

Comput Methods Programs Biomed 2021 Apr 23;202:106005. Epub 2021 Feb 23.

Aerospace Information Research Institute, Chinese Academy of Sciences (AIRCAS), Beijing, China; Personalized Management of Chronic Respiratory Disease, Chinese Academy of Medical Sciences, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Background And Objective: In recent years, people have been exploring methods for biometric identification through electrocardiogram (ECG) signals. Under the same psychological pressure state, biometric identification through ECG signals is a traditional verification method. However, ECG signals are affected by changes in psychological stress, and ECG-Based biometric under different psychological stress states are still challenging. In this paper, we propose a method combining manual and automatic features for ECG-based biometric under different psychological stress states. And propose a new indicator Stress Classification Coefficient (SCC) that assesses the effect of different psychological stress on heart rate variability (HRV) features.

Methods: In our method, we obtain manual features to be a three-step process: first, HRV features obtained from the ECG signals. Second, based on HRV features, the mental state of the experimental subjects is assessed by using the Gaussian mixture model (GMM). Finally, use cluster centers to process the original HRV features to reduce the Stress Classification Coefficient (SCC). Also, the one-dimensional convolutional neural network is constructed to automatically extract the implied features of ECG signals. Finally, the manual feature and the automatic feature are combined, and the final recognition result is obtained through the support vector machine (SVM) model. The major attribute of the proposed method is that it can perform ECG biometric under different psychological stress states. The combination of manual and automatic features expands the application scenarios of ECG-based biometric.

Results: Based on this method, we used the Montreal stress model with calculation experiment in the laboratory to induce stress on 23 healthy students (10 women and 13 men, aged 20-37), and obtain their ECG signals under different stress conditions. Through this method to recognize the above data, an average recognition rate of more than 95% can be achieved, the average F1 score is 0.97.

Conclusions: The proposed method in this article is a promising approach to deal with the effects of different psychological stresses on ECG-Based biometric. It provides the possibility of ECG-Based biometric under different psychological stress.
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http://dx.doi.org/10.1016/j.cmpb.2021.106005DOI Listing
April 2021

Extracellular HMGB1 promotes CD44 expression in hepatocellular carcinoma via regulating miR-21.

Aging (Albany NY) 2021 03 4;13(6):8380-8395. Epub 2021 Mar 4.

Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China.

As a member of damage-associated molecular patterns (DAMPs), extracellular high-mobility group box 1 (HMGB1) plays a critical role in hepatocellular carcinoma (HCC) progression. Cluster differentiation 44 (CD44) has been demonstrated to participate in HCC progression. However, the relationship between extracellular HMGB1 and CD44 remains unclear. In this study, our results indicated that extracellular HMGB1 promoted the invasion, sphere formation and EMT process of HCC by increasing CD44 expression, which was dependent on miR-21. Moreover, miR-21 upregulated CD44 expression via activating OCT4/TGF-β1 signaling. Finally, we demonstrated the activation of Rage/JNK signaling caused by extracellular HMGB1 was responsible for miR-21 overexpression. Together, these findings reveal an important role of extracellular HMGB1 in HCC progression through upregulating miR-21/CD44.
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http://dx.doi.org/10.18632/aging.202649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034936PMC
March 2021

Precision ejection of microfluidic droplets into air with a superhydrophobic outlet.

Lab Chip 2021 04;21(8):1484-1491

Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA. and California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA, USA and Chan Zuckerberg Biohub, San Francisco, CA, USA.

Dispensing micron-scale droplets from a suspended nozzle is important for applications in bioprinting, analytical chemistry, and pharmaceutical formulation. Here, we describe a general approach to eject droplets from microfluidic devices using superhydrophobic patterning; this facilitates release of wetted fluids, allowing droplets to break contact with channel surfaces and travel along regular paths to achieve a printing accuracy of ∼3 μm. We demonstrate the utility of the approach by using it to print droplets of varied composition from a microfluidic mixing device. Our approach is compatible with common fabrication techniques making it applicable to devices configured for diverse applications.
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http://dx.doi.org/10.1039/d0lc01327gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189694PMC
April 2021

Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics.

Commun Biol 2021 03 1;4(1):265. Epub 2021 Mar 1.

Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.

Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible for rare cell populations (e.g., circulating tumor cells (CTCs)). Here we report a surfactant-assisted one-pot sample preparation coupled with mass spectrometry (MS) method termed SOP-MS for label-free global single-cell proteomics. SOP-MS capitalizes on the combination of a MS-compatible nonionic surfactant, n-Dodecyl-β-D-maltoside, and hydrophobic surface-based low-bind tubes or multi-well plates for 'all-in-one' one-pot sample preparation. This 'all-in-one' method including elimination of all sample transfer steps maximally reduces surface adsorption losses for effective processing of single cells, thus improving detection sensitivity for single-cell proteomics. This method allows convenient label-free quantification of hundreds of proteins from single human cells and ~1200 proteins from small tissue sections (close to ~20 cells). When applied to a patient CTC-derived xenograft (PCDX) model at the single-cell resolution, SOP-MS can reveal distinct protein signatures between primary tumor cells and early metastatic lung cells, which are related to the selection pressure of anti-tumor immunity during breast cancer metastasis. The approach paves the way for routine, precise, quantitative single-cell proteomics.
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http://dx.doi.org/10.1038/s42003-021-01797-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921383PMC
March 2021

Evaluation of China's public health system response to COVID-19.

J Glob Health 2021 Jan 16;11:05004. Epub 2021 Jan 16.

School of Labor and Human Resources, Renmin University of China, Beijing, China.

Background: The focus of the study is to assess the advantages and shortcomings of China's public health system in the process of the COVID-19 prevention and to discuss the future reform of China's public health system.

Methods: By searching literature and reports related to the COVID-19 prevention of China, we compared the prevention effectiveness with the prevention policies in the process of the COVID-19 prevention.

Results: China's public health system can effectively combine national power to maximize the effectiveness of pandemic prevention. It improved the pandemic prevention ability of communities continuously and promoted the fairness of prevention. Traditional Chinese Medicine has also been used in pandemic prevention, which reduces the drug resistance of the virus. At the same time, the combination of the disease diagnosis and the Internet has reduced the spread speed of the pandemic. China's public health system also has some problems in response to the COVID-19, such as the shortage of medical resources, insufficient alerts, the low efficiency of reporting to superior government and the shortage of reward and punishment system for pandemic prevention.

Conclusions: China's practice and efforts of the COVID-19 prevention can provide experience for other countries to improve their public health systems and accelerate the end of the COVID-19 pandemic.
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http://dx.doi.org/10.7189/jogh.11.05004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897426PMC
January 2021

Drug-induced liver injury associated with antiseizure medications from the FDA Adverse Event Reporting System (FAERS).

Epilepsy Behav 2021 04 21;117:107832. Epub 2021 Feb 21.

Rutgers-Robert Wood Johnson Medical School, Department of Medicine, Division of Gastroenterology and Hepatology, 125 Paterson Street Suite 5100B, New Brunswick, NJ 08901, United States; Center for Liver Diseases and Liver Masses, Rutgers-Robert Wood Johnson Medical School, 125 Paterson Street Suite 5100B, New Brunswick, NJ 08901, United States.

Purpose: Treatment with antiseizure medications (ASMs) confers a risk of drug-induced liver injury (DILI), especially for older ASMs. We sought to quantify recent reports of DILI attributed to both older and newer generation ASMs and survey newly marketed ASMs for hepatotoxicity in a large post-marketing database.

Methods: We queried over 2.6 million adverse event reports made to the FDA Adverse Event Reporting System (FAERS) database between July 1, 2018 and March 31, 2020 for DILI due to ASMs commonly used in clinical practice. Patient characteristics and outcomes were assessed. We calculated the reporting odds ratio (ROR) of DILI for each individual ASM versus all non-ASM reports.

Results: A total of 2175 DILI cases were attributed to an ASM during the study period. 97.2% of these were designated as serious reactions, which include death, hospitalization, disability, and other life-threatening outcomes. A number of older and newer generation ASMs were associated with DILI, specifically: carbamazepine (ROR 2.92), phenobarbital (ROR 2.91), oxcarbazepine (ROR 2.58), phenytoin (ROR 2.40), valproate (ROR 2.22), lamotrigine (ROR 2.06), clobazam (ROR 1.67), levetiracetam (ROR 1.56), and diazepam (ROR 1.53). However, increased odds of DILI were not seen with zonisamide, perampanel, stiripentol, lacosamide, clonazepam, pregabalin, felbamate, eslicarbazepine, cannabidiol, topiramate, gabapentin, ethosuximide, brivaracetam, or primidone. Vigabatrin, tiagabine, and rufinamide all had zero reports of DILI.

Conclusions: The majority of newer generation ASMs were not significantly associated with DILI. Future studies utilizing FAERS in conjunction with other data sources will be critical for the ongoing surveillance of DILI, particularly as newly marketed ASMs continue to enter into widespread clinical use.
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http://dx.doi.org/10.1016/j.yebeh.2021.107832DOI Listing
April 2021

Influence of sex and menopausal status on the relationship between metabolic syndrome and coronary artery calcification: a Chinese community-based cross-sectional study.

Menopause 2021 02 22;28(5):546-553. Epub 2021 Feb 22.

Central Hospital Affiliated to Shandong First Medical University, Jinan, P.R. China.

Objective: To evaluate the association between metabolic syndrome and coronary artery calcification according to different sex and menopausal status.

Methods: This cross-sectional study included 2,704 adults from the Jidong community (Tangshan, China) recruited from July 2013 to August 2014. Adults aged ≥40 years with no cardiovascular disease and with coronary artery calcification score data were included. Metabolic syndrome was defined according to the 2005 International Diabetes Federation standard. Coronary artery calcification score was determined using the Agatston method. The associations between metabolic syndrome and coronary artery calcification prevalence were evaluated using logistic regression.

Results: In the multivariable regression analysis, metabolic syndrome was associated with coronary artery calcification (odds ratio: 1.34, 95% confidence interval: 1.04-1.71, P = 0.021). When stratified by sex, metabolic syndrome was positively associated with coronary artery calcification prevalence in female participants (odds ratio: 2.79, 95% confidence interval: 1.96-3.96, P < 0.001), whereas no association was observed in male participants. Furthermore, metabolic syndrome was associated with a higher prevalence of coronary artery calcification (P < 0.001) independent of adjustment for covariates in postmenopausal women than in premenopausal women, and coronary artery calcification prevalence increased with an increase in conditions related to metabolic syndrome.

Conclusions: Our findings indicate that metabolic syndrome in postmenopausal women is associated with a higher prevalence of coronary artery disease than in premenopausal women and men.
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http://dx.doi.org/10.1097/GME.0000000000001750DOI Listing
February 2021

Interdisciplinary influences in headache literature: A network citation analysis of PubMed Central articles.

Headache 2021 Jan 8;61(1):143-148. Epub 2020 Dec 8.

Department of Neurology, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ, USA.

Background: Non-headache literature inevitably influences headache research, but the way this interdisciplinary interaction occurs has seldom been evaluated.

Objective: Utilizing network analysis techniques within the PubMed Central (PMC) database, we illustrate a novel method by which to identify and characterize the important non-headache literature with significant impact within the headache world.

Methods: Using the National Center for Biotechnology Information E-utilities application programing interface and custom backend software, all PMC articles containing the words "headache(s)" and/or "migraine(s)" in the title were identified. This generated a list of "seed articles" to represent the body of primary headache literature. Articles referenced by the seeds were then found, generating the list of articles with one degree of separation from the seeds (first-degree neighbors). This was iterated twice more to find the second- and third-degree neighbors. A directed network graph was generated for each level of separation using these articles and their referential connections. The hyperlink-induced topic search (HITS) and PageRank algorithms were used on these graphs to find the top 50 articles in the network (hub and authority rank via HITS, general rank via PageRank). Removing seed articles from the ranked lists left the influential non-headache articles at each level of separation.

Results: We extracted 6890 seed articles. The first-, second-, and third-degree models contained 16,451, 105,496, and 431,748 articles, respectively. As expected, most first-degree neighbors were part of the seed group (headache literature). Using HITS, at the second degree, only two seed articles were found in the top 50 hubs (none in the authorities); the majority of non-seed articles were basic neuroscience, involving ion channel function or cell signaling. At the third degree, there were no seeds and all articles involved imaging/structure of brain connectivity networks. PageRank gave more varied results, with 35/50 second-degree articles being seeds, and the remainder a mixture of articles describing rating scales (3), epidemiology/disease burden (3), basic statistical/trial methods (3), and mixed basic science (6). At the third degree, five were seeds; non-seed articles were represented heavily by genomic mapping studies, brain connectivity networks, and ion channel/neurotransmitter studies.

Conclusion: This work demonstrates the value of network citation analysis in the identification of interdisciplinary influences on headache medicine. Articles found with this technique via HITS identified and grouped basic science applicable to headache medicine at the molecular scale (ion channels/transmitters), and whole-brain scale (connectivity networks). Both groups have direct clinical correlates, with the former implicating pharmacological targets, and the latter implicating functional neuroanatomy and pathophysiology of various headache disorders. Likely, in-depth analysis of the whole network (rather than the top 50) would reveal further clusters where the relationship to headache may not be as immediately obvious. This may not only help to guide ongoing work, but also identify new targets where seemingly unrelated work may have future applications in headache management.
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http://dx.doi.org/10.1111/head.14022DOI Listing
January 2021

Efficacy and safety of rescue angioplasty and/or stenting for acute large artery occlusion with underlying intracranial atherosclerosis: A systematic review and meta-analysis.

Clin Neurol Neurosurg 2021 Apr 8;203:106538. Epub 2021 Feb 8.

Department of Pediatrics, Tianjin First Central Hospital, Tianjin, 300192, China. Electronic address:

Purpose: Intracranial angioplasty and/or stenting implantation is an important rescue treatment for the management of intracranial atherosclerosis-related occlusion (ICAS-O) after mechanical thrombectomy failure, but its safety and efficacy remain unclear. We investigated the safety and efficacy of rescue intracranial angioplasty and/or stenting for emergent large artery occlusion (LAO) with underlying ICAS.

Methods: We searched for relevant full-text articles in EMBASE, PubMed and the Cochrane Central Register of Controlled Trials from inception to March 1, 2020. We calculated the odds ratios (ORs) using random-effects models for symptomatic intracranial hemorrhage (sICH), mortality, recanalization rate and favorable clinical outcome at 90 days between ICAS-O group treated by rescue therapy and Non ICAS-O group. RStudio software 1.3.959 was used to perform this meta-analysis.

Results: Ten studies were included with a total of 1639 patients, of which 450 (27.5 %) were in the ICAS-O group treated with intracranial angioplasty and/or stenting, and 1189 (72.5 %) were in the Non ICAS-O group. Overall, intracranial angioplasty and/or stenting did not improve the recanalization rate (OR, 0.67 [0.26-1.76]; p = 0.419) or favorable functional outcome (OR, 1.01 [0.64-1.58]; p = 0.97) in patients with underlying ICAS-O, and the risk of sICH (OR, 0.99 [0.59-1.68]; p = 0.983) and mortality (OR, 1.26 [0.87-1.83]; p = 0.225) did not significantly differ between ICAS-O and Non ICAS-O.

Conclusions: From these observational study results, rescue intracranial angioplasty and/or stenting seems safe in patients with emergent LAO after attempted thrombectomy, but further rigorous studies are warranted to confirm its efficacy.
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http://dx.doi.org/10.1016/j.clineuro.2021.106538DOI Listing
April 2021

Short-term but not long-term high fat diet feeding protects against pressure overload-induced heart failure through activation of mitophagy.

Life Sci 2021 May 16;272:119242. Epub 2021 Feb 16.

School of Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.

Aims: Recent studies have shown that enhancement of fatty acid utilization through feeding animals a high fat diet (HFD) attenuated cardiac dysfunction in heart failure (HF). Here, we aimed to examine the temporal effects of HFD feeding on cardiac function in mice with heart failure and its underlying mechanism.

Main Methods: Pressure overload-induced HF was established via transverse aortic constriction (TAC) surgery. After surgery, the mice were fed on either normal diet or HFD for 8 or 16 weeks.

Key Findings: HFD feeding exerted opposite effects on cardiac function at different time points post-surgery. Short-term HFD feeding (8 wk) protected the heart against pressure overload, inhibiting cardiac hypertrophy and improving cardiac function, while long-term HFD feeding (16 wk) aggravated cardiac dysfunction in TAC mice. Short-term HFD feeding elevated cardiac fatty acid utilization, while long-term HFD feeding showed no significant effects on cardiac fatty acid utilization in TAC mice. Specifically, an increase in cardiac fatty acid utilization was accompanied with activated mitophagy and improved mitochondrial function. Palmitic acid treatment (400 μM, 2 h) stimulated fatty acid oxidation and mitophagy in neonatal myocytes. Mechanistically, fatty acid utilization stimulated mitophagy through upregulation of Parkin. Cardiac-specific knockdown of Parkin abolished the protective effects of short-term HFD feeding on cardiac function in TAC mice.

Significances: These results suggested that short-term but not long-term HFD feeding protects against pressure overload-induced heart failure through activation of mitophagy, and dietary fat intake should be used with caution in treatment of heart failure.
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http://dx.doi.org/10.1016/j.lfs.2021.119242DOI Listing
May 2021
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