Publications by authors named "Peng Zhang"

5,150 Publications

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A gene-coated microneedle patch based on industrialized ultrasonic spraying technology with a polycation vector to improve antitumor efficacy.

J Mater Chem B 2021 Jun 23. Epub 2021 Jun 23.

MOE Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang Province, P. R. China.

A coated microneedle patch is a reliable way to load gene on a surface as a transdermal gene delivery platform. But there are many limitations to the traditional methods to fabricate a coated microneedle patch, such as the fact that they are time consuming or the difficulty in controlling the loading content. In this research, ultrasonic spraying technology, as an industrialized production method, was first used to fabricate a gene-coated microneedle patch. First, the p53 expression plasmid (p53 DNA) was ultrasonically sprayed on a polycaprolactone (PCL) microneedle patch ([email protected]). To promote the transfection efficiency, polycation polyethylenimine (PEI), as a vector, was then ultrasonically sprayed on [email protected] ([email protected]@MNP). From the experimental results, although two layers were sprayed step by step, no obvious stratification could be observed. The vector PEI interweaved with genes and inhibited the gene release profile, but it changed the released naked genes to positively charged complexes, which would promote gene transfection efficiency. In subsequent in vivo experiments, the anti-tumor efficacy of the "[email protected]@MNP treated group" could reach 84.7%, although it had the lowest gene release profile. In contrast, the anti-tumor efficacy of the "intravenous injection group" and "[email protected] treated group" was only 24.3% and 59.3%, respectively. Overall, [email protected]@MNP was a safe and efficient device to treat the subdermal tumor. Ultrasonic spraying technology provided an industrialized method to fabricate the coated microneedle patch as a transdermal gene/drug delivery platform.
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http://dx.doi.org/10.1039/d1tb00512jDOI Listing
June 2021

Intermediate-term results of a prospective, multicenter study on remote programming sacral neuromodulation for refractory overactive bladder.

Transl Androl Urol 2021 May;10(5):1966-1975

Urology Department, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences Beijing, China.

Background: To evaluate the efficacy and safety of a novel remote programming sacral neuromodulation (SNM) system (BetterStim system) in the treatment of patients with refractory overactive bladder (OAB) for 3 years.

Methods: A total of 8 centers in China enrolled 84 patients with OAB. Following test stimulation 70 patients underwent implantation using BetterStim. All patients returned for follow-up at 3 and 6 months postoperatively. After that, the visits were conducted by telephone. Outcomes of voiding diaries, the overactive bladder symptom score (OABSS), questionnaires regarding OAB-related quality of life (OAB-qol), and adverse events (AEs) were evaluated at each visit. The clinical therapeutic success was defined as ≥50% improvement from baseline in any of the voiding diary variables or average voids/day return to normal voiding (<8 voids/day). The analysis is a modified Completers analysis.

Results: At the 36-month follow-up, the success rate was 79% for overall OAB symptoms, 69% for urge urinary incontinence (UUI) and 42% for urgency frequency (UF). The average number of voids/day decreased from 29.2±14.9 at baseline to 17.6±11.2 at 3 years, the average volume/void increased from 94.7±54.2 to 151.4±80.8 mL, the urgency reduced from 3.0±1.4 to 1.8±1.7 (all P<0.001). For patients with urge incontinence, mean leaking episodes/day decreased from 8.1±7.6 at baseline to 2.1±3.5 at three years (P<0.05). The devices were explanted in 8 (11.4%) patients. There were no remote programming-related AEs or device-related serious AEs that occurred.

Conclusions: The Intermediate-term results demonstrated that BetterStim SNM system with remote programming is safe and effective for patients with refractory OAB.
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http://dx.doi.org/10.21037/tau-21-43DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185673PMC
May 2021

p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal Differentiation.

Int J Stem Cells 2021 Jun 30. Epub 2021 Jun 30.

The Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease of Sichuan Province, Insti.

Background And Objectives: Manipulating different signaling pathways via small molecules could efficiently induce cardiomyocytes from human induced pluripotent stem cells (hiPSC). However, the effect of transcription factors on the hiPSC-directed cardiomyocytes differentiation remains unclear. Transcription factor, p53 has been demonstrated indispensable for the early embryonic development and mesendodermal differentiation of embryonic stem cells (ESC). We tested the hypothesis that p53 promotes cardiomyocytes differentiation from human hiPSC.

Methods And Results: Using the well-characterized GiWi protocol that cardiomyocytes are generated from hiPSC via temporal modulation of Wnt signaling pathway by small molecules, we demonstrated that forced expression of p53 in hiPSC remarkably improved the differentiation efficiency of cardiomyocytes from hiPSC, whereas knockdown endogenous p53 decreased the yield of cardiomyocytes. This p53-mediated increased cardiomyocyte differentiation was mediated through WNT3, as evidenced by that overexpression of p53 upregulated the expression of WNT3, and knockdown of p53 decreased the WNT3 expression. Mechanistic analysis showed that the increased cardiomyocyte differentiation partially depended on the amplified mesendodermal specification resulted from p53-mediated activation of WNT3-mediated Wnt signaling. Consistently, endogenous WNT3 knockdown significantly ameliorated mesendodermal specification and subsequent cardiomyocyte differentiation.

Conclusions: These results provide a novel insight into the potential effect of p53 on the development and differentiation of cardiomyocyte during embryogenesis.
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http://dx.doi.org/10.15283/ijsc21051DOI Listing
June 2021

Growing Rice () Aerobically Reduces Phytotoxicity, Uptake, and Transformation of CeO Nanoparticles.

Environ Sci Technol 2021 Jun 22. Epub 2021 Jun 22.

Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

This study compared the impact and uptake of root-administered CeO nanoparticles (NPs) in rice growing under flooded and aerobic soil conditions, which are two water regimes commonly used for rice cultivation. CeO NPs at 100 mg/kg improved photosynthesis and plant growth by reducing the oxidative damage and enhancing plant tolerance to stress, while a higher concentration (500 mg/kg) of CeO NPs negatively affected plant growth. More significant effects were observed under the flooded condition than under the aerobic condition. CeO NPs of 100 and 500 mg/kg resulted in 78% and 70% higher accumulation of Ce in shoots under the flooded condition compared to the aerobic condition. CeO NPs partially transformed to Ce(III) species in soils and plants under both conditions. A higher extent of transformation under the flooded condition, which was partly attributed to the lower soil pH and redox potential under the flooded condition, leads to higher plant uptake of Ce. A higher extent of transformation in rhizosphere soil was observed. A higher plant transpiration rate (TR) under flooded conditions resulted in a higher accumulation of CeO species in shoots. This study, for the first time, reported that water regimes influenced the biotransformation of CeO NPs and their uptake and impact in rice plants.
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http://dx.doi.org/10.1021/acs.est.0c08813DOI Listing
June 2021

Apatinib with doxorubicin and ifosfamide as neoadjuvant therapy for high-risk soft tissue sarcomas: a retrospective cohort study.

Invest New Drugs 2021 Jun 22. Epub 2021 Jun 22.

Department of Bone and Soft Tissue, the Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Henan Province, Zhengzhou, 450008, China.

Background: There is a need to establish an effective neoadjuvant therapy for soft tissue sarcomas (STSs). We previously showed that apatinib, administered in combination with doxorubicin-based chemotherapy, improves the efficacy of treatment. This study aimed to clarify the effectiveness and safety of apatinib combined with doxorubicin and ifosfamide (AI) neoadjuvant chemotherapy for STSs.

Methods: This retrospective study included patients with STS who received neoadjuvant therapy and surgery between January 2016 and January 2019. The patients were divided into two treatment groups: AI + apatinib group and AI group (doxorubicin + ifosfamide).

Results: The study included 74 patients (AI + apatinib: 26, AI: 48) with STS. There were significant between-group differences in objective response rates (53.85% vs. 29.17%, p = 0.047) and the average change in target lesion size from baseline (-40.46 ± 40.30 vs. -16.31 ± 34.32, p = 0.008). The R0 rate (84.62% vs. 68.75%; p = 0.170) and 2-year disease-free survival (73.08% vs. 62.50%, p = 0.343) were similar across groups. Finally, the rates of neoadjuvant therapy-related adverse effects and postoperative complications were similar in both groups (p > 0.05).

Conclusion: Apatinib plus doxorubicin and ifosfamide regimen is safe and effective as neoadjuvant therapy for patients with STS. However, the significantly improved preoperative ORR observed after neoadjuvant therapy did not translate into a significantly improved R0 rate and 2-year DFS. Prospective, well-powered studies are warranted to determine the long-term efficacy and optimal application of these protocols.
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http://dx.doi.org/10.1007/s10637-021-01139-wDOI Listing
June 2021

Targeting the HIF-1α-IGFBP2 axis therapeutically reduces IGF1-AKT signaling and blocks the growth and metastasis of relapsed anaplastic Wilms tumor.

Oncogene 2021 Jun 21. Epub 2021 Jun 21.

Division of Cancer and Immunology Research, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA.

For patients with anaplastic Wilms tumor (WiT), metastasis and recurrence are common, and prognosis is generally poor. Novel therapies are needed to improve outcomes for patients with this high-risk WiT. A potential contributor to WiT development is constitutive activation of AKT by insulin-like growth factor 1 (IGF1) and its receptor (IGF1R) signaling pathway, but the complete underlying mechanism remains unclear. Here, we demonstrate that the hypoxia-inducible factor 1α (HIF-1α)-IGF binding protein 2 (IGFBP2) axis and the tumor-specific IGF1A are key players for constitutive activation of IGF1-AKT signaling leading to the tumor malignancy. HIF-1α and IGFBP2 are highly expressed in a majority of WiT patient samples. Deficiency of either HIF-1α or IGFBP2 or IGF1 in the tumor cells significantly impairs tumor growth and nearly abrogates metastasis in xenografted mice. Pharmacologic targeting of HIF-1α by echinomycin delivered via nanoliposomes can efficiently restrain growth and metastasis of patient-derived relapsed anaplastic WiT xenografts. Liposomal echinomycin is more potent and effective in inhibiting WiT growth than vincristine in an anaplastic WiT mouse model, and eliminates metastasis by suppressing HIF-1α targets and the HIF-1α-IGFBP2 axis, which governs IGF1-AKT signaling.
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http://dx.doi.org/10.1038/s41388-021-01907-1DOI Listing
June 2021

Compact dual-wavelength vertical-external-cavity surface-emitting laser with simple elements.

Opt Express 2021 May;29(11):16572-16583

Dual-wavelength lasers with separation from several nanometers to tens of nanometers at 1 µm waveband are attractive light sources for terahertz generation. This work reports a compact dual-wavelength vertical-external-cavity surface-emitting laser with simple elements. The gain chip is regularly designed and epitaxially grown, and the laser cavity is the most common straight line cavity. By the use of a blade as the tuning element in cavity, the laser wavelength can be tuned continuously, and the stable dual-wavelength oscillation can be obtained when the blade is situated at a certain position. The total output power of 85 mW, which is limited by our pump source, is produced when the laser wavelengths are at 961 nm and 970 nm. We have also analyzed the evolution mechanism and the stability of this dual-wavelength laser.
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http://dx.doi.org/10.1364/OE.423074DOI Listing
May 2021

Wide-range, ultra-compact, and high-sensitivity ring resonator biochemical sensor with CMOS-compatible hybrid plasmonic waveguide.

Opt Express 2021 Jun;29(12):19058-19067

A ring resonator-based biochemistry sensor with a wide range, ultra-compact footprint, and high sensitivity is proposed, which utilizes a suspended slot hybrid plasmonic (SSHP) waveguide. The waveguide consists of a suspended Si nanowire separated from a Cu metal surface by a nanoscale air gap. The hybridization of fundamental mode of a Si channel waveguide with the surface plasmon polariton (SPP) mode of Cu-Si interface achieves a strong light confinement, high waveguide sensitivity (Sw), and low optical loss, showing a great potential in integrated optical sensor. The sensitivity, the detection limit and the detection range of the SSHP waveguide-based biochemistry sensor with a miniaturized radius of 1 µm are numerically demonstrated as 458.1 nm/RIU, 3.7 × 10 RIU and 0.225 RIU, respectively. These superior performances as well as the fully CMOS compatibility enable the integrated optical sensing applications.
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http://dx.doi.org/10.1364/OE.428159DOI Listing
June 2021

Low-loss silicon nitride strip-slot mode converter based on MMI.

Opt Express 2021 Jun;29(12):19049-19057

Slot waveguide has attracted a lot of attention due to its ability to confine light in the low refractive index region, while strip waveguide acts as the basic component of guiding light due to its relatively low optical loss. In the multifunctional photonic integrated chips, it is critical to achieve the low loss transition between the strip waveguide and the slot waveguide. In this work, a silicon nitride strip-slot mode converter with high efficiency, large bandwidth, and large fabrication tolerance are proposed and demonstrated through the numerical investigation and experiments. The coupling efficiency of the mode converter is up to - 0.1 dB (97.7%), which enables the extremely low transition loss between the strip waveguide and the slot waveguide. Moreover, the fabrication process of silicon nitride photonic devices with high performance is introduced, which is fully compatible with the CMOS technology. Photonic devices based on silicon nitride with the characteristics of the low optical loss and the temperature insensitivity represent a new paradigm in realizing silicon-based photonic multifunctional chips.
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http://dx.doi.org/10.1364/OE.427802DOI Listing
June 2021

Computational study of novel inhibitory molecule, 1-(4-((2,3)-3-amino-2-hydroxy-4-phenylbutyl)piperazin-1-yl)-3-phenylurea, with high potential to competitively block ATP binding to the RNA dependent RNA polymerase of SARS-CoV-2 virus.

J Biomol Struct Dyn 2021 Jun 21:1-19. Epub 2021 Jun 21.

Laboratory for Translational Chemistry and Drug Discovery, Department of Chemistry, Hansraj College, University of Delhi, Delhi, India.

For coronaviruses, RNA-dependent RNA polymerase (RdRp) is an essential enzyme that catalyses the replication from RNA template and therefore remains an attractive therapeutic target for anti-COVID drug discovery. In the present study, we performed a comprehensive screening for 16,776 potential molecules from recently established drug libraries based on two important pharmacophores (3-amino-4-phenylbutan-2-ol and piperazine). Based on initial assessment, 4042 molecules were obtained suitable as drug candidates, which were following Lipinski's rule. Molecular docking implemented for the analysis of molecular interactions narrowed this number of compounds down to 19. Subsequent to screening filtering criteria and considering the critical parameters docking score and MM-GBSA binding free energy, 1-(4-((2,3)-3-amino-2-hydroxy-4-phenylbutyl)piperazin-1-yl)-3-phenylurea (compound ) was accomplished to score highest in comparison to the remaining 18 shortlisted drug candidates. Notably, compound displayed higher docking score (-8.069 kcal/mol) and MM-GBSA binding free energy (-49.56 kcal/mol) than the control drug, remdesivir triphosphate, the active form of remdesivir as well as adenosine triphosphate. Furthermore, a molecular dynamics simulation was carried out (100 ns), which substantiated the candidacy of compound as better inhibitor. Overall, our systematic study predicts the potential of compound to exhibit a more favourable specific activity than remdesivir triphosphate. Hence, we suggest compound 1 as a novel potential drug candidate, which should be considered for further exploration and validation of its potential against SARS-CoV-2 in wet lab experimental studies.Communicated by Ramasawamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2021.1940281DOI Listing
June 2021

A Non-invasive Model for Predicting Liver Inflammation in Chronic Hepatitis B Patients With Normal Serum Alanine Aminotransferase Levels.

Front Med (Lausanne) 2021 4;8:688091. Epub 2021 Jun 4.

Department of Gastroenterology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Chronic hepatitis B (CHB) patients with normal alanine aminotransferase (ALT) levels are at risk of disease progression. Currently, liver biopsy is suggested to identify this population. We aimed to establish a non-invasive diagnostic model to identify patients with significant liver inflammation. A total of 504 CHB patients who had undergone liver biopsy with normal ALT levels were randomized into a training set ( = 310) and a validation set ( = 194). Independent variables were analyzed by stepwise logistic regression analysis. After the predictive model for diagnosing significant inflammation (Scheuer's system, G ≥ 2) was established, a nomogram was generated. Discrimination and calibration aspects of the model were measured using the area under the receiver operating characteristic curve (AUC) and assessment of a calibration curve. Clinical significance was evaluated by decision curve analysis (DCA). The model was composed of 4 variables: aspartate aminotransferase (AST) levels, γ-glutamyl transpeptidase (GGT) levels, hepatitis B surface antigen (HBsAg) levels, and platelet (PLT) counts. Good discrimination and calibration of the model were observed in the training and validation sets (AUC = 0.87 and 0.86, respectively). The best cutoff point for the model was 0.12, where the specificity was 83.43%, the sensitivity was 77.42%, and the positive likelihood and negative likelihood ratios were 4.67 and 0.27, respectively. The model's predictive capability was superior to that of each single indicator. This study provides a non-invasive approach for predicting significant liver inflammation in CHB patients with normal ALT. Nomograms may help to identify target patients to allow timely initiation of antiviral treatment.
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http://dx.doi.org/10.3389/fmed.2021.688091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213212PMC
June 2021

Effect of acupuncture on sleep quality and neurological function in stroke patients with sleep apnea syndrome.

Am J Transl Res 2021 15;13(5):5635-5640. Epub 2021 May 15.

Department of Acupuncture, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin, China.

Objective: To investigate the effect of acupuncture on sleep quality and neurological function in stroke patients with sleep apnea syndrome (SAS).

Methods: In this prospective study, a total of 88 stroke patients with SAS were randomized into two groups: observation group (44 cases; patients received the western medicine treatment combined with acupuncture) and control group (44 cases; patients were given western medicine treatment only). All patients in both groups were treated for three weeks. The clinical efficacy, sleep quality, apnea-hypopnea index (AHI), cognitive function, neuron-specific enolase (NSE) and S100 calcium binding protein β (S100β) levels before and after treatment were compared between patients in the two groups.

Results: Compared with those before treatment, the sleep latency, sleep duration, sleep efficiency and minimal oxygen saturation (SaO) increased, while the longest apnea time and AHI decreased in both groups after treatment. More significant changes were found in the observation group (all P<0.05). After treatment, the overall effective rate in the observation group was higher than that in the control group (P<0.05); serum levels of NSE and S100β in both groups were lower than those before treatment, and the levels of the observation group were lower than those of the control group (all P<0.05); Montreal Cognitive Assessment (MoCA) scores in both groups were higher than those before treatment, and scores of the observation group were higher than those of the control group (all P<0.05).

Conclusion: Western medicine treatment combined with acupuncture can significantly relieve the clinical symptoms of stroke patients with SAS and improve sleep quality and neurological function. Therefore, it is worthy of clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205691PMC
May 2021

Overexpression of SHMT2 Predicts a Poor Prognosis and Promotes Tumor Cell Growth in Bladder Cancer.

Front Genet 2021 4;12:682856. Epub 2021 Jun 4.

Department of Gastroenterology, Guizhou Provincial People's Hospital, Guiyang, China.

SHMT2 was overexpressed in many tumors, however, the role of SHMT2 in bladder cancer (BLCA) remains unclear. We first analyzed the expression pattern of SHMT2 in BLCA using the TNMplot, Oncomine, the Cancer Genome Atlas (TCGA), and the Gene Expression Omnibus (GEO) databases. Next, the association between SHMT2 expression and overall survival (OS)/disease-free survival (DFS) in BLCA patients were analyzed using TCGA and PrognoScan database. The correlation between SHMT2 expression and clinicopathology was determined using TCGA database. Furthermore, the genes co-expressed with SHMT2 and their underlying molecular function in BLCA were explored based on the Oncomine database, Metascape and gene set enrichment analysis (GSEA). Finally, the effects of SHMT2 on cell proliferation, cell cycle, and apoptosis were assessed using experiments. As a results, SHMT2 was significantly overexpressed in BLCA tissues and cells compared to normal bladder tissues and cells. A high SHMT2 expression predicts a poor OS of BLCA patients. In addition, SHMT2 expression was higher in patients with a high tumor grade and in those who were older than 60 years. However, the expression of SHMT2 was not correlated with gender, tumor stage, lymph node stage, and distant metastasis stage. Finally, overexpression of SHMT2 promoted BLCA cell proliferation and suppressed apoptosis, the silencing of SHMT2 significantly inhibited BLCA cell proliferation by impairing the cell cycle, and promoting apoptosis. SHMT2 mediates BLCA cells growth by regulating STAT3 signaling. In summary, SHMT2 regulates the proliferation, cell cycle and apoptosis of BLCA cells, and may act as a candidate therapeutic target for BLCA.
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http://dx.doi.org/10.3389/fgene.2021.682856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212063PMC
June 2021

Plate-to-Layer BiMoO/MXene-Heterostructured Anode for Lithium-Ion Batteries.

Nanomicro Lett 2019 Sep 25;11(1):81. Epub 2019 Sep 25.

State Key Laboratory of Organic-Inorganic Composites, Beijing Key Laboratory of Electrochemical Process and Technology for Materials, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.

BiMoO is a potentially promising anode material for lithium-ion batteries (LIBs) on account of its high theoretical capacity coupled with low desertion potential. Due to low conductivity and large volume expansion/contraction during charge/discharge cycling of BiMoO, effective modification is indispensable to address these issues. In this study, a plate-to-layer BiMoO/TiCT (MXene) heterostructure is proposed by electrostatic assembling positive-charged BiMoO nanoplates on negative-charged MXene nanosheets. MXene nanosheets in the heterostructure act as a highly conductive substrate to load and anchor the BiMoO nanoplates, so as to improve electronic conductivity and structural stability. When the mass ratio of MXene is optimized to 30%, the BiMoO/MXene heterostructure exhibits high specific capacities of 692 mAh g at 100 mA g after 200 cycles and 545.1 mAh g with 99.6% coulombic efficiency at 1 A g after 1000 cycles. The results provide not only a high-performance lithium storage material, but also an effective strategy that could address the intrinsic issues of various transition metal oxides by anchoring them on MXene nanosheets to form heterostructures and use as anode materials for LIBs.
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http://dx.doi.org/10.1007/s40820-019-0312-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770671PMC
September 2019

Electrostatic Self-assembly of 0D-2D SnO Quantum Dots/TiCT MXene Hybrids as Anode for Lithium-Ion Batteries.

Nanomicro Lett 2019 Aug 2;11(1):65. Epub 2019 Aug 2.

School of Materials Science and Engineering, Beijing Key Laboratory of Environmental Science and Engineering, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.

MXenes, a new family of two-dimensional (2D) materials with excellent electronic conductivity and hydrophilicity, have shown distinctive advantages as a highly conductive matrix material for lithium-ion battery anodes. Herein, a facile electrostatic self-assembly of SnO quantum dots (QDs) on TiCT MXene sheets is proposed. The as-prepared SnO/MXene hybrids have a unique 0D-2D structure, in which the 0D SnO QDs (~ 4.7 nm) are uniformly distributed over 2D TiCT MXene sheets with controllable loading amount. The SnO QDs serve as a high capacity provider and the "spacer" to prevent the MXene sheets from restacking; the highly conductive TiCT MXene can not only provide efficient pathways for fast transport of electrons and Li ions, but also buffer the volume change of SnO during lithiation/delithiation by confining SnO QDs between the MXene nanosheets. Therefore, the 0D-2D SnO QDs/MXene hybrids deliver superior lithium storage properties with high capacity (887.4 mAh g at 50 mA g), stable cycle performance (659.8 mAh g at 100 mA g after 100 cycles with a capacity retention of 91%) and excellent rate performance (364 mAh g at 3 A g), making it a promising anode material for lithium-ion batteries.
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http://dx.doi.org/10.1007/s40820-019-0296-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770891PMC
August 2019

D2A U-Net: Automatic segmentation of COVID-19 CT slices based on dual attention and hybrid dilated convolution.

Comput Biol Med 2021 Jun 2;135:104526. Epub 2021 Jun 2.

School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China; Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, 100191, China. Electronic address:

Coronavirus Disease 2019 (COVID-19) has become one of the most urgent public health events worldwide due to its high infectivity and mortality. Computed tomography (CT) is a significant screening tool for COVID-19 infection, and automatic segmentation of lung infection in COVID-19 CT images can assist diagnosis and health care of patients. However, accurate and automatic segmentation of COVID-19 lung infections is faced with a few challenges, including blurred edges of infection and relatively low sensitivity. To address the issues above, a novel dilated dual attention U-Net based on the dual attention strategy and hybrid dilated convolutions, namely D2A U-Net, is proposed for COVID-19 lesion segmentation in CT slices. In our D2A U-Net, the dual attention strategy composed of two attention modules is utilized to refine feature maps and reduce the semantic gap between different levels of feature maps. Moreover, the hybrid dilated convolutions are introduced to the model decoder to achieve larger receptive fields, which refines the decoding process. The proposed method is evaluated on an open-source dataset and achieves a Dice score of 0.7298 and recall score of 0.7071, which outperforms the popular cutting-edge methods in the semantic segmentation. The proposed network is expected to be a potential AI-based approach used for the diagnosis and prognosis of COVID-19 patients.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169238PMC
June 2021

TRIM28 regulates SARS-CoV-2 cell entry by targeting ACE2.

Cell Signal 2021 Jun 17;85:110064. Epub 2021 Jun 17.

Central Laboratory, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China; Institute of Translational Cardiovascular Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China; Department of Cardiovascular Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China. Electronic address:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019, it binds to angiotensin-converting enzyme 2 (ACE2) to enter into human cells. The expression level of ACE2 potentially determine the susceptibility and severity of COVID-19, it is thus of importance to understand the regulatory mechanism of ACE2 expression. Tripartite motif containing 28 (TRIM28) is known to be involved in multiple processes including antiviral restriction, endogenous retrovirus latency and immune response, it is recently reported to be co-expressed with SARS-CoV-2 receptor in type II pneumocytes; however, the roles of TRIM28 in ACE2 expression and SARS-CoV-2 cell entry remain unclear. This study showed that knockdown of TRIM28 induces ACE2 expression and increases pseudotyped SARS-CoV-2 cell entry of A549 cells and primary pulmonary alveolar epithelial cells (PAEpiCs). In a co-culture model of NK cells and lung epithelial cells, our results demonstrated that NK cells inhibit TRIM28 and promote ACE2 expression in lung epithelial cells, which was partially reversed by depletion of interleukin-2 and blocking of granzyme B in the co-culture medium. Furthermore, TRIM28 knockdown enhanced interferon-γ (IFN-γ)- induced ACE2 expression through a mechanism involving upregulating IFN-γ receptor 2 (IFNGR2) in both A549 and PAEpiCs. The upregulated ACE2 induced by TRIM28 knockdown and co-culture of NK cells was partially reversed by dexamethasone in A549 cells. Our study identified TRIM28 as a novel regulator of ACE2 expression and SARS-CoV-2 cell entry.
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http://dx.doi.org/10.1016/j.cellsig.2021.110064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213541PMC
June 2021

Mechanistic analysis of multiple processes controlling solar-driven HO synthesis using engineered polymeric carbon nitride.

Nat Commun 2021 Jun 17;12(1):3701. Epub 2021 Jun 17.

Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea.

Solar-driven hydrogen peroxide (HO) production presents unique merits of sustainability and environmental friendliness. Herein, efficient solar-driven HO production through dioxygen reduction is achieved by employing polymeric carbon nitride framework with sodium cyanaminate moiety, affording a HO production rate of 18.7 μmol h mg and an apparent quantum yield of 27.6% at 380 nm. The overall photocatalytic transformation process is systematically analyzed, and some previously unknown structural features and interactions are substantiated via experimental and theoretical methods. The structural features of cyanamino group and pyridinic nitrogen-coordinated soidum in the framework promote photon absorption, alter the energy landscape of the framework and improve charge separation efficiency, enhance surface adsorption of dioxygen, and create selective 2e oxygen reduction reaction surface-active sites. Particularly, an electronic coupling interaction between O and surface, which boosts the population and prolongs the lifetime of the active shallow-trapped electrons, is experimentally substantiated.
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http://dx.doi.org/10.1038/s41467-021-24048-1DOI Listing
June 2021

[Comparison of plasma levels and immunoactivities of different forms of circulating-free DNA in systemic lupus erythematosus patients].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jul;37(7):633-640

Department of Laboratory Medicine, First Affiliated Hospital, Wannan Medical College, Wuhu 241001, China. *Corresponding author, E-mail:

Objective To detect and analyze the plasma levels and immunoactivities of different forms of circulating-free DNA (cfDNA) in systemic lupus erythematosus (SLE) patients. Methods The study enrolled 58 patients with SLE, 66 patients with other autoimmune diseases (non-SLE) and 60 healthy individuals. Total cfDNA, exosome cfDNA and immune complex cfDNA were extracted from the plasma and detected using a fluorescence method. Overall methylation levels of cfDNA were measured. Macrophages and dendritic cells induced in vitro were co-cultured with exosomes or immune complexes derived from SLE patients pre-treated with deoxyribonuclease 1-like 3(DNASE1L3) or immunoglobulin G (IgG) specific degradation enzyme or none. Then, cytokines and cell surface activation markers were detected by the multiple fluorescent microsphere assay. Results Among the three groups, SLE patients had the highest levels of exosomes and immune complex cfDNA, followed by non-SLE patients, and no significant differences were found in the simple cfDNA. The methylation levels of different types of cfDNA (except simple cfDNA) in the plasma of SLE patients were significantly lower than that of the non-SLE group and healthy individuals. Non-SLE patients and healthy individuals presented no significant differences for the methylation levels of all cfDNA types. After DNASE1L3 and IgG enzyme treatment, the macrophages and dendritic cells stimulated by exosomes and immune complex cfDNA secreted significantly lower levels of cytokines including MIP-1a, MIP-1b, IL-4, IL-1β, IL-2, IL-1ra, MCP-1, IL-6, IL-9, IL-8, TNF-α and IFN-α. Compared with IgG enzyme treatment, DNASE1L3-treated exosomes and immune complex induced the lower level of cytokines secretion. The expression of CD80, CD86 and CD40 on the surfaces of macrophages and dendritic cells stimulated with exosomes and immune complexes pre-treated with DNASE1L3 enzyme were significantly reduced, but the expression of CD86 on the macrophages was slightly changed. The expression of CD80, CD86 and CD40 showed no difference in the two types of cells stimulated with exosomes and immune complexes pre-treated with IgG enzymes or none, but an overall downward trend existed indeed. Conclusion Exosomes and immune complex cfDNA increase significantly in the plasma of SLE patients, and they can stimulate strong responses of macrophages and dendritic cells.
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July 2021

Macroscopic rods from assembled colloidal particles of hydrothermally carbonized glucose and their use as templates for silicon carbide and tricopper silicide.

J Colloid Interface Sci 2021 Jun 14;602:480-489. Epub 2021 Jun 14.

Department of Materials and Environmental Chemistry, Stockholm University Svante Arrhenius väg 16 C SE 10691 Stockholm, Sweden. Electronic address:

Self-aggregated colloids can be used for the preparation of materials, and we studied long rod-like aggregates formed on the evaporation of water from dispersed particles of colloidal hydrochar. The monodispersed hydrochar particles (100-200 nm) were synthesized by the hydrothermal carbonization of glucose and purified through dialysis. During the synthesis they formed colloidal dispersions which were electrostatically stable at intermediate to high pH and at low ion strengths. On the evaporation of water, macroscopically large rods formed from the dispersions at intermediate pH conditions. The rods formed at the solid-water interface orthogonally oriented with respect to the drying direction. Pyrolysis rendered the rods highly porous without qualitatively affecting their shape. A Cu-Si alloy was reactively infiltrated into the in-situ pyrolyzed hydrochars and composites of tricopper silicide (CuSi)-silicon carbide (SiC)/carbon formed. During this process, the Si atoms reacted with the C atoms, which in turned caused the alloy to wet and further react with the carbon. The shape of the underlying carbon template was maintained during the reactions, and the formed composite preparation was subsequently calcined into a CuSi-SiC-based replica of the rod-like assemblies of carbon-based colloidal particles. Transmission and scanning electron microscopy, and X-ray diffraction were used to study the shape, composition, and structure of the formed solids. Further studies of materials prepared with reactive infiltration of alloys into self-aggregated and carbon-based solids can be justified from a perspective of colloidal science, as well as the explorative use of hydrochar prepared from real biomass, exploration of the compositional space in relation to the reactive infiltration, and applications of the materials in catalysis.
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http://dx.doi.org/10.1016/j.jcis.2021.06.016DOI Listing
June 2021

Human STAT3 variants underlie autosomal dominant hyper-IgE syndrome by negative dominance.

J Exp Med 2021 Aug 17;218(8). Epub 2021 Jun 17.

Garvan Institute of Medical Research, Darlinghurst, Australia.

Most patients with autosomal dominant hyper-IgE syndrome (AD-HIES) carry rare heterozygous STAT3 variants. Only six of the 135 in-frame variants reported have been experimentally shown to be dominant negative (DN), and it has been recently suggested that eight out-of-frame variants operate by haploinsufficiency. We experimentally tested these 143 variants, 7 novel out-of-frame variants found in HIES patients, and other STAT3 variants from the general population. Strikingly, all 15 out-of-frame variants were DN via their encoded (1) truncated proteins, (2) neoproteins generated from a translation reinitiation codon, and (3) isoforms from alternative transcripts or a combination thereof. Moreover, 128 of the 135 in-frame variants (95%) were also DN. The patients carrying the seven non-DN STAT3 in-frame variants have not been studied for other genetic etiologies. Finally, none of the variants from the general population tested, including an out-of-frame variant, were DN. Overall, our findings show that heterozygous STAT3 variants, whether in or out of frame, underlie AD-HIES through negative dominance rather than haploinsufficiency.
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http://dx.doi.org/10.1084/jem.20202592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217968PMC
August 2021

Transcriptomic profiling of tumor microenvironment reveals distinct immune subgroups of metastatic melanoma and its potential implications for immunotherapy.

J Genet Genomics 2021 Apr 14. Epub 2021 Apr 14.

Division of Immunotherapy, Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2021.03.008DOI Listing
April 2021

Spatial state distribution and phase transition of non-uniform water in soils: Implications for engineering and environmental sciences.

Adv Colloid Interface Sci 2021 Jun 9;294:102465. Epub 2021 Jun 9.

State Key Laboratory of Frozen Soil Engineering, Beilu'He Observation and Research Station on Tibetan Plateau, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; Department of Earth, Atmospheric, and Planetary Sciences, Purdue University, West Lafayette, IN 47907, USA; Center for Permafrost (CENPERM), University of Copenhagen, DK1350 Copenhagen, Denmark.

The physical behaviors of water in the interface are the fundamental to discovering the engineering properties and environmental effects of aqueous porous media (e.g., soils). The pore water pressure (PWP) is a key parameter to characterize the pore water state (PWS) and its phase transition in the micro interface. Traditionally, the water in the interface is frequently believed to be uniform, negative in pressure and tensile based on macroscopic tests and Gibbs interface model. However, the water in the interface is a non-uniform and compressible fluid (part of tensile and part of compressed), forming a spatial profile of density and PWP depending on its distance from the substrate interface. Herein, we introduced the static and dynamic theory methods of non-uniform water based on diffuse interface model to analyze non-uniform water state dynamics and water density and PWP. Based on the theory of non-uniform water, we gave a clear stress analysis on soil water and developed the concepts of PWS, PWP and matric potential in classical soil mechanics. In addition, the phase transition theory of non-uniform water is also examined. In nature, the generalized Clausius-Clapeyron equation (GCCE) is consistent with Clapeyron equation. Therefore, a unified interpretation is proposed to justify the use of GCCE to represent frozen soil water dynamics. Furthermore, the PWP description of non-uniform water can be well verified by some experiments focusing on property variations in the interface area, including the spatial water density profile and unfrozen water content variations with decreasing temperature and other factors. In turn, PWP spatial distribution of non-uniform water and its states can well explain some key phenomena on phase transition during ice or hydrate formation, including the discrepancies of phase transition under a wide range of conditions.
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http://dx.doi.org/10.1016/j.cis.2021.102465DOI Listing
June 2021

Comparative Analysis of Hydrogen-Bonding Vibrations of Ice VI.

ACS Omega 2021 Jun 25;6(22):14442-14446. Epub 2021 May 25.

School of Space Science and Physics, Shandong University, Weihai 264209, Shandong, China.

It is difficult to investigate the hydrogen-bonding dynamics of hydrogen-disordered ice VI. Here, we present a comparative method based on our previous study of its counterpart hydrogen-ordered phase, ice XV. The primitive cell of ice XV is a 10 molecule unit, and the vibrational normal modes were analyzed individually. We constructed an 80 molecule supercell of ice VI to mimic the periodic unit and performed first-principles density functional theory calculations. As the two vibrational spectra show almost identical features, we compared the molecular translation vibrations. Inspired by the phonon analysis of ice XV, we found that the vibrational modes in the translation band of ice VI are classifiable into three groups. The lowest-strength vibration modes represent vibrations between two sublattices that lack hydrogen bonding. The highest-strength vibration modes represent the vibration of four hydrogen bonds of one molecule. The middle-strength vibration modes mainly represent the molecular vibrations of only two hydrogen bonds. Although there are many overlapping stronger and middle modes, there are only two main peaks in the inelastic neutron scattering (INS) spectra. This work explains the origin of the two main peaks in the far-infrared region of ice VI and illustrates how to analyze a hydrogen-disordered ice structure.
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http://dx.doi.org/10.1021/acsomega.1c01315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190920PMC
June 2021

Editorial: Application of Systems Biology in Molecular Characterization and Diagnosis of Cancer.

Front Mol Biosci 2021 28;8:668146. Epub 2021 May 28.

Department of Surgery, School of Medicine, University of Maryland, College Park, MD, United States.

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http://dx.doi.org/10.3389/fmolb.2021.668146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193925PMC
May 2021

TPX2 Enhanced the Activation of the HGF/ETS-1 Pathway and Increased the Invasion of Endocrine-Independent Prostate Carcinoma Cells.

Front Oncol 2021 28;11:618540. Epub 2021 May 28.

Department of Urology, Chinese People's Liberation Army (PLA) General Hospital/Chinese PLA Medical Academy, Beijing, China.

The prognosis for endocrine-independent prostate carcinoma is still poor due to its highly metastatic feature. In the present work, TPX2 (the targeting protein for Xklp2), which is known as a micro-tubulin interacted protein, was identified as a novel coactivator of ETS-1, a transcription factor that plays a central role in mediating the metastasis of human malignancies. TPX2 enhanced the transcription factor activation of ETS-1 and increased the expression of ETS-1's downstream metastasis-related genes, such as or , induced by HGF (hepatocyte growth factor), a typical agonist of the HGF/c-MET/ETS-1 pathway. The protein-interaction between TPX2 and ETS-1 was examined using immunoprecipitation (IP). TPX2 enhanced the accumulation of ETS-1 in the nuclear and the recruitment of its binding element (EST binding site, EBS) located in the promoter region of its downstream gene, . Moreover, TPX2 enhanced the or invasion of a typical endocrine-independent prostate carcinoma cell line, PC-3. Therefore, TPX2 enhanced the activation of the HGF/ETS-1 pathway to enhance the invasion of endocrine-independent prostate carcinoma cells and thus it would be a promising target for prostate carcinoma treatment.
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http://dx.doi.org/10.3389/fonc.2021.618540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193931PMC
May 2021

Driving high quantum yield NIR emission through proquinoidal linkage motifs in conjugated supermolecular arrays.

Chem Sci 2020 Aug 5;11(31):8095-8104. Epub 2020 Aug 5.

Department of Chemistry, French Family Science Center, Duke University 124 Science Drive Durham North Carolina 27708-0346 USA

High quantum yield NIR fluorophores are rare. Factors that drive low emission quantum yields at long wavelength include the facts that radiative rate constants increase proportional to the cube of the emission energy, while nonradiative rate constants increase in an approximately exponentially with decreasing S-S energy gaps (in accordance with the energy gap law). This work demonstrates how the proquinoidal BTD building blocks can be utilized to minimize the extent of excited-state structural relaxation relative to the ground-state conformation in highly conjugated porphyrin oligomers, and shows that 4-ethynylbenzo[][1,2,5]thiadiazole () units that terminate -to- ethyne-bridged (porphinato)zinc () arrays, and 4,7-diethynylbenzo[][1,2,5]thiadiazole () spacers that are integrated into the backbone of these compositions, elucidate new classes of impressive NIR fluorophores. We report the syntheses, electronic structural properties, and emissive characteristics of neoteric , , and fluorophores. Absolute fluorescence quantum yield () measurements, acquired using a calibrated integrating-sphere-based measurement system, demonstrate that these supermolecules display extraordinary values that range from 10-25% in THF solvent, and between 28-36% in toluene solvent over the 700-900 nm window of the NIR. These studies underscore how the regulation of proquinoidal conjugation motifs can be exploited to drive excited-state dynamical properties important for high quantum yield long-wavelength fluorescence emission.
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http://dx.doi.org/10.1039/d0sc03446kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163388PMC
August 2020

Hierarchical-pore UiO-66 modified with Ag for π-complexation adsorption desulfurization.

J Hazard Mater 2021 May 27;418:126247. Epub 2021 May 27.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. Electronic address:

Adsorption desulfurization represents an alternative technology for the effective removal of thiophenic compounds from fuels. Metal-organic frameworks have been the ideal candidates for the adsorptive desulfurization of fuel due to the high surface areas. Pristine UiO-66 is thought to be appropriate for the removal of small thiophenic compounds. This work developed a new type of hierarchical-pore (micro and mesopores) UiO-66 with a higher specific surface area and porosity for the removal of larger adsorbates using MOF-5 as the template. To enhance adsorption desulfurization performance, the Ag-exchanged hierarchical-pore UiO-66 (HP-UiO-66-SOAg) with π-complexation was synthesized by the ion-exchange method. The HP-UiO-66-SOAg samples were characterized by FTIR, XRD, SEM, TEM, and N adsorption-desorption isotherms. Compared with the original UiO-66, the HP-UiO-66-SOAg has a higher specific surface area, pore volume, and pore size. The static adsorption experiments showed that HP-UiO-66-SOAg had a high adsorption capacity for thiophene and benzothiophene. Moreover, the HP-UiO-66-SOAg sample still exhibits high adsorptive performance in the presence of toluene. The regeneration results show that about 90% of the initial adsorption capacity of HP-UiO-66-SOAg can be regenerated after four cycles.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126247DOI Listing
May 2021

A risk score system for predicting complicated appendicitis and aid decision-making for antibiotic therapy in acute appendicitis.

Ann Palliat Med 2021 May 24. Epub 2021 May 24.

Department of Gastrointestinal Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.

Background: Exclusive antibiotic therapy is a feasible treatment option for uncomplicated appendicitis, but the pre-treatment diagnosis of uncomplicated appendicitis is challenging. This study aimed to develop a risk score system to predict complicated appendicitis and aiding decision-making regarding antibiotic therapy for acute appendicitis.

Methods: The risk score system for predicting complicated appendicitis was constructed and validated by a surgical therapy cohort (n=543). Furthermore, we applied an independent antibiotic treatment cohort (n=169) to verify whether the risk score system could guide antibiotic treatment decision-making in patients with acute appendicitis (AA).

Results: A total of 543 patients were included in the surgical therapy cohort and was split into the primary (n=375) and validation (n=168) cohorts with repeated random sampling. In the primary cohort, multivariate analysis confirmed that periappendiceal fat stranding (PFS, P<0.001, OR =67.80), the C-reactive protein level (CRP ≥38 mg/L, P<0.001, OR =5.77) and the neutrophil-to-lymphocyte ratio (NLR ≥7, P<0.001, OR =3.51) were independent risk factors for complicated appendicitis. The PFS, CRP and NLR scores were 10.0, 4.0 and 3.0 points, respectively. Fourteen patients (3.7%, 14/375) and seven patients (4.2%, 7/168) with pathologically confirmed complicated appendicitis were classified as having uncomplicated appendicitis in the primary and validation cohorts based on the risk score system, respectively. In the independent antibiotic treatment cohort (n=169), the failure rate of antibiotic treatment was 49.2% and 5.3% for the risk score system predicted complicated AA and uncomplicated AA. Furthermore, the predictive accuracy of the risk score system for antibiotic treatment failure as measured by the area under the curve (AUC) was 0.823 (95% CI: 0.757-0.878).

Conclusions: We found that the proposed risk score system based on biological and CT features not only enables the accurate identification of complicated appendicitis patients before pre-treatment but also serves to guide antibiotic treatment decisions.
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http://dx.doi.org/10.21037/apm-21-26DOI Listing
May 2021

4-OI Attenuates Carbon Tetrachloride-Induced Hepatic Injury via Regulating Oxidative Stress and the Inflammatory Response.

Front Pharmacol 2021 25;12:651444. Epub 2021 May 25.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The liver is an important metabolic organ, and acute liver injury (ALI) is potentially lethal. Itaconate, a metabolic intermediate from the tricarboxylic acid cycle, showed emerging anti-oxidative and anti-inflammation properties, and an accumulating protective effect in multiple diseases, but its role in ALI still needs to be further explored. Here we established an ALI model induced by carbon tetrachloride in mice. Our results showed that 4-Octyl itaconate (OI), a derivate of itaconate, mitigated hepatic damage by improving liver function, reducing histopathological damage, and decreasing the death of hepatocytes. Additionally, OI decreased myeloperoxidase and thiobarbituric acid reactive substances (TBARS) levels in the ALI model. OI also inhibited the inflammatory response by reducing pro-inflammatory cytokine secretion (IL-6, TNF-α, IL-1β, and MCP-1) and infiltration of macrophages and neutrophils in the ALI model. However, administration of ML385, a specified Nrf2 inhibitor, eliminated the protective properties of OI in the CCl4-induced liver injury model by increasing hepatic damage and oxidative stress. Furthermore, OI increased the expression and nuclear translocation of Nrf2 and elevated the expression of heme oxygenase-1 and NAD(P)H quinone oxidoreductase 1, while knockdown of Nrf2 eliminated these effects in murine hepatocyte NCTC 1469 under CCl4 treatment. Moreover, we found that OI reduced serum High-mobility group box 1 (HMGB1) levels in CCl4-treated mice. Finally, OI inhibited nuclear translocation of factor-kappa B (NF-𝜅B) and inflammatory cytokine production in murine macrophages. In conclusion, these results indicated that OI ameliorated CCl4-induced ALI by mitigating oxidative stress and the inflammatory response. The possible mechanism was associated with the elevation of Nrf2 nuclear translocation and inhibition of HMGB1 mediated the nuclear translocation of NF-𝜅B.
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http://dx.doi.org/10.3389/fphar.2021.651444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185275PMC
May 2021