Publications by authors named "Peng Zeng"

161 Publications

Photocatalytic hydrogen evolution over a nickel complex anchoring to thiophene embedded g-CN.

J Colloid Interface Sci 2021 Mar 19;596:75-88. Epub 2021 Mar 19.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, PR China. Electronic address:

Evolution of hydrogen from water by utilizing solar energy and photocatalysts is one of the most promising ways to solve energy crisis. However, designing a cost-effective and stable photocatalyst without any noble metals is of vital importance for this process. Herein, an extremely active molecular complex cocatalyst NiL(Cl) is successfully designed. After being covalently linked to thiophene-embedded polymeric carbon nitride (TPCN), the hybrid catalyst NiL(Cl)/TPCN exhibits extraordinary H production activity of 95.8 μmol h without Pt (λ ≥ 420 nm), together with a remarkable apparent quantum yield of 6.68% at 450 nm. In such a composite catalyst, the embedded π-electron-rich thiophene-ring not only extends the π-conjugated system to enhance visible light absorption, but also promotes the charge separation through electron-withdrawing effect. It turns out that the CN covalent bonds formed between NiL(Cl) and TPCN skeleton accelerate the transfer of electrons to the Ni active sites. Our finding reveals that the strategy of embedding π-electron-rich compounds to graphitic carbon nitride provides potentials to develop excellent photocatalysts. The strong covalent combination of molecular complexes cocatalyst onto organic semiconductors represents an important step towards designing noble-metal-free photocatalysts with superior activity and high stability for visible light driven hydrogen evolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.03.080DOI Listing
March 2021

Highly Effective Trapping-Conversion Interface Based on Nickel-Modified Versatile Carbon Skeleton Enabled High-Performance Li-S Battery.

ACS Appl Mater Interfaces 2021 Apr 6. Epub 2021 Apr 6.

Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, School of Chemistry, Xiangtan University, Xiangtan 411105, Hunan, China.

The development of comprehension in the mechanism of lithium-sulfur (Li-S) batteries creates more opportunities and potential for the application of interlayer. However, the viable design of versatile interlayer to retard the shuttling effects and improve the sluggish kinetics is still a focus and paramount challenge. Herein, we present a tentacles-like metallic nickel-modified and nitrogen-doped carbon skeleton (NCS) to serve as adsorbent and catalyst in the lithium-sulfur battery (LSBs). The carbonized skeleton and derived carbon tubes jointly construct conductive networks and adequate ion pathways. Meanwhile, abundant metallic nickel nanoparticles synergistically build a multifunctional interface with polar networks for the fixation and conversion of polysulfides, giving rise to significant improvement of cyclic stability and reaction kinetics of LSBs. As a result, the Li-S batteries using NCS as an interlayer could possess superior electrochemical performance including cyclic stability, high specific capacity (1204.8 mAh g at 0.2C, 998.7 mAh g at 1C), and good Coulombic efficiency. More importantly, even with the areal sulfur loading of up to 6.1 and 7.5 mg cm, it still demonstrates superior electrochemical performance with the areal capacity of 4.2 and 5.9 mAh cm with steady cycling, respectively. In conclusion, we confirm this work provides a promising way to explore and expand the application of metal nanoparticles in interlayers for advanced Li-S batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c01600DOI Listing
April 2021

The Main Alkaloids in and Their Anti-Alzheimer's Disease Mechanism Determined by a Network Pharmacology Approach.

Int J Mol Sci 2021 Mar 31;22(7). Epub 2021 Mar 31.

Key Laboratory of Neurological Disease of National Education Ministry, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Alzheimer's disease (AD) is a growing concern in modern society, and effective drugs for its treatment are lacking. (UR) and its main alkaloids have been studied to treat neurodegenerative diseases such as AD. This study aimed to uncover the key components and mechanism of the anti-AD effect of UR alkaloids through a network pharmacology approach. The analysis identified 10 alkaloids from UR based on HPLC that corresponded to 90 anti-AD targets. A potential alkaloid target-AD target network indicated that corynoxine, corynantheine, isorhynchophylline, dihydrocorynatheine, and isocorynoxeine are likely to become key components for AD treatment. KEGG pathway enrichment analysis revealed the Alzheimers disease (hsa05010) was the pathway most significantly enriched in alkaloids against AD. Further analysis revealed that 28 out of 90 targets were significantly correlated with Aβ and tau pathology. These targets were validated using a Gene Expression Omnibus (GEO) dataset. Molecular docking studies were carried out to verify the binding of corynoxine and corynantheine to core targets related to Aβ and tau pathology. In addition, the cholinergic synapse (hsa04725) and dopaminergic synapse (hsa04728) pathways were significantly enriched. Our findings indicate that UR alkaloids directly exert an AD treatment effect by acting on multiple pathological processes in AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22073612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036964PMC
March 2021

Vegetation coverage change of the demonstration area of ecologically friendly development in the Yangtze River Delta, China based on GEE and BRT during 1984-2019.

Ying Yong Sheng Tai Xue Bao 2021 Mar;32(3):1033-1044

School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.

The demonstration area of ecologically friendly development in the Yangtze River Delta is a major national strategy area, and thus eco-environmental protection should be a priority for the sustainable development. Exploring the spatial-temporal variations of franctional vegetation coverage (FVC) is conducive to accurate assessing the ecological quality of environment, which is of great significance to regional sustainable development. In this study, the characteristics and trends of spatial-temporal variation of vegetation cover during 1984-2019 in the demonstration area were analyzed based on Google Earth Engine (GEE). The effects of different ecological factors on FVC were quantified by the boosted regression tree (BRT). Results showed that,the changing trend of FVC in the study area shifted from decreasing to increasing trend from 1984 to 2019. The spatial distribution of FVC in the study area varied with both stages and regions. FVC was mainly degraded. Compared with the year 1984, the area of vegetation degradation and improvement in 2019 accounted for 49.8% and 12.8%, respectively. The vegetation degradation mainly occurred in the north of Wujiang, south of Jiashan, and northeast of Qingpu. Human activities severely weakened the influence of natural factors on FVC. Our findings suggested that the GEE is an effective tool for monitoring the dynamics of vegetation coverage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13287/j.1001-9332.202103.011DOI Listing
March 2021

Nano-FeO-modified biochar promotes the formation of iron plaque and cadmium immobilization in rice root.

Chemosphere 2021 Mar 9;276:130212. Epub 2021 Mar 9.

College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China; Hunan Engineering Laboratory for Control of Rice Quality and Safety, Central South University of Forestry and Technology, Changsha, 410004, China. Electronic address:

Rice as a paddy field crops, iron-containing materials application could induce its iron plaque formation, thereby affecting cadmium (Cd) transportation in the rhizosphere and its uptake in root. In this study, a hydroponic experiment was conducted to investigate the effects of three exogenous iron materials, namely nano-FeO-modified biochar (BC-Fe), chelated iron (EDTA-Fe), and ferrous sulfate (FeSO), on the iron plaque formation on the surface of rice root, and to investigate the effects of formed iron plaque on the absorption, migration, and transportation of Cd and Fe in rice plant. The results showed that yellow-brown and brown iron plaque was formed on surface cells of the Fe-treated rice root, and some black particles were embedded in the iron plaque formed by BC-Fe. The proportion of crystallized iron plaque (31.8%-35.9%) formed by BC-Fe was much higher than that formed by EDTA-Fe and FeSO. The Cd concentrations in the crystallized iron plaque formed by BC-Fe were 7.64-13.0 mg·kg, and increased with the increasing of Fe concentrations in the plaque. The Cd translocation factor from root to stem (TF) and the Cd translocation factor from stem to leaf (TF) with BC-Fe treatment decreased by 84.7% and 80.0%, respectively. The results demonstrated that application BC-Fe promoted the formation of iron plaque and enhanced the sequestration of Cd and Fe in roots, thus reduced the transportation and accumulation of Cd in aerial rice tissues.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130212DOI Listing
March 2021

New estimate of chemical weathering rate in Xijiang River Basin based on multi-model.

Sci Rep 2021 Mar 11;11(1):5728. Epub 2021 Mar 11.

Institute of Karst Geology CAGS/Key Laboratory of Karst Dynamics, MNR & GZAR, Guilin, 541004, China.

Hydrochemistry and Sr isotope compositions were measured in water samples collected during high- and low-water periods from the main stream and tributaries of the Xijiang River Basin in southern China. The primary weathering end-members were analyzed and calculated using the multi-model combination and classic hydrogeochemical method. During the high-water period, structural factors were found to be the main factors controlling chemical weathering in the basin, whereas anthropogenic activity and other random factors had a negligible influence. During the low-water period, both structural and random factors controlled chemical weathering. Through path-model and semi-variance analyses, we determined and quantified the relationship between the main weathering sources, whose results were stable; this is consistent with the inversion model. The total dissolved substances were mainly derived from carbonate weathering, which was approximately 76% (0-96%) while silicate weathering accounted for only 14% (5-19%). The inversion model results showed that the optimum silicate weathering rate was 7.264-35.551 × 10 mol/km/year, where carbonic acid was the main factor that induces weathering. The CO flux consumed by rock weathering in the basin during the study period was 150.69 × 10 mol/year, while the CO flux consumed by carbonic acid weathering of carbonate (CCW) and silicate rocks (CSW) was 144.47 and 29.45 × 10 mol/year, respectively. The CO flux produced by HSO weathered carbonate (SCW) was 23.23 × 10 mol/year.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-84602-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952734PMC
March 2021

COVID-19: A Comparative Study of Population Aggregation Patterns in the Central Urban Area of Tianjin, China.

Int J Environ Res Public Health 2021 02 22;18(4). Epub 2021 Feb 22.

School of Architecture, Tianjin University, Tianjin 300272, China.

When a public health emergency occurs, a potential sanitation threat will directly change local residents' behavior patterns, especially in high-density urban areas. Their behavior pattern is typically transformed from demand-oriented to security-oriented. This is directly manifested as a differentiation in the population distribution. This study based on a typical area of high-density urban area in central Tianjin, China. We used Baidu heat map (BHM) data to calculate full-day and daytime/nighttime state population aggregation and employed a geographically weighted regression (GWR) model and Moran's I to analyze pre-epidemic/epidemic population aggregation patterns and pre-epidemic/epidemic population flow features. We found that during the COVID-19 epidemic, the population distribution of the study area tended to be homogenous clearly and the density decreased obviously. Compared with the pre-epidemic period: residents' demand for indoor activities increased (average correlation coefficient of the floor area ratio increased by 40.060%); traffic demand decreased (average correlation coefficient of the distance to a main road decreased by 272%); the intensity of the day-and-night population flow declined significantly (its extreme difference decreased by 53.608%); and the large-living-circle pattern of population distribution transformed to multiple small-living circles. This study identified different space utilization mechanisms during the pre-epidemic and epidemic periods. It conducted the minimum living security state of an epidemic-affected city to maintain the operation of a healthy city in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph18042135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927029PMC
February 2021

The Clinical Features of Graves' Orbitopathy with Elevated Intraocular Pressure.

J Ophthalmol 2021 25;2021:9879503. Epub 2021 Jan 25.

Department of Ophthalmology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.

Background: To investigate the clinical characteristics of Graves' orbitopathy (GO) with elevated intraocular pressure (IOP) using the European Group of Graves' Orbitopathy (EUGOGO) system.

Methods: In this retrospective study, the clinical data of GO patients with elevated IOP (≥21 mmHg) were collected in Sun Yat-sen Memorial Hospital from January 2010 to June 2016. The demographic characteristics, clinical history of thyroid disease and GO, and ocular examination data were evaluated, and the activity and severity of GO were classified.

Results: Data were collected from 58 eyes of 39 patients. The durations of thyroid disease and GO were 15.9 ± 18.9 months and 7.5 ± 6.2 months, respectively. The average IOP was 24.8 ± 5.3 mmHg (range: 21-55 mmHg). No significant difference in IOP was observed between active and inactive eyes. Eight eyes (13.8%), 29 eyes (50.0%), and 21 eyes (36.2%) were graded as mild, moderate-severe, and sight-threatening disease, respectively, according to the EUGOGO classification. The IOP was not significantly different among the three EUGOGO grades. No glaucomatous optic nerve damage or visual field defects were found.

Conclusion: Increased IOP was evident for every grade of GO severity and activity of the EUGOGO system. IOP, glaucomatous optic nerve damage, and visual fields must be evaluated regularly during follow-up evaluations, regardless of the degree of activity and severity of GO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9879503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850838PMC
January 2021

RBMX suppresses tumorigenicity and progression of bladder cancer by interacting with the hnRNP A1 protein to regulate PKM alternative splicing.

Oncogene 2021 Feb 9. Epub 2021 Feb 9.

Center for Reproductive Medicine, Qingyuan People's Hospital, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan, Guangdong, China.

The prognosis for patients with metastatic bladder cancer (BCa) is poor, and it is not improved by current treatments. RNA-binding motif protein X-linked (RBMX) are involved in the regulation of the malignant progression of various tumors. However, the role of RBMX in BCa tumorigenicity and progression remains unclear. In this study, we found that RBMX was significantly downregulated in BCa tissues, especially in muscle-invasive BCa tissues. RBMX expression was negatively correlated with tumor stage, histological grade and poor patient prognosis. Functional assays demonstrated that RBMX inhibited BCa cell proliferation, colony formation, migration, and invasion in vitro and suppressed tumor growth and metastasis in vivo. Mechanistic investigations revealed that hnRNP A1 was an RBMX-binding protein. RBMX competitively inhibited the combination of the RGG motif in hnRNP A1 and the sequences flanking PKM exon 9, leading to the formation of lower PKM2 and higher PKM1 levels, which attenuated the tumorigenicity and progression of BCa. Moreover, RBMX inhibited aerobic glycolysis through hnRNP A1-dependent PKM alternative splicing and counteracted the PKM2 overexpression-induced aggressive phenotype of the BCa cells. In conclusion, our findings indicate that RBMX suppresses BCa tumorigenicity and progression via an hnRNP A1-mediated PKM alternative splicing mechanism. RBMX may serve as a novel prognostic biomarker for clinical intervention in BCa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41388-021-01666-zDOI Listing
February 2021

Neonatal cortical astrocytes possess intrinsic potential in neuronal conversion in defined media.

Acta Pharmacol Sin 2021 Feb 5. Epub 2021 Feb 5.

Shanghai Key Laboratory of Signaling and Disease Research, Laboratory of Receptor-based Bio-medicine, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China.

Astrocytes are multifunctional brain cells responsible for maintaining the health and function of the central nervous system. Accumulating evidence suggests that astrocytes might be complementary source across different brain regions to supply new neurons during adult neurogenesis. In this study, we found that neonatal mouse cortical astrocytes can be directly converted into neurons when exposed to neurogenic differentiation culture conditions, with insulin being the most critical component. Detailed comparison studies between mouse cortical astrocytes and neuronal progenitor cells (NPCs) demonstrated the converted neuronal cells originate indeed from the astrocytes rather than NPCs. The neurons derived from mouse cortical astrocytes display typical neuronal morphologies, express neuronal markers and possess typical neuronal electrophysiological properties. More importantly, these neurons can survive and mature in the mouse brain in vivo. Finally, by comparing astrocytes from different brain regions, we found that only cortical astrocytes but not astrocytes from other brain regions such as hippocampus and cerebellum can be converted into neurons under the current condition. Altogether, our findings suggest that neonatal astrocytes from certain brain regions possess intrinsic potential to differentiate/transdifferentiate into neurons which may have clinical relevance in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41401-020-00586-0DOI Listing
February 2021

[Network pharmacology-based study of the therapeutic mechanism of resveratrol for Alzheimer's disease].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jan;41(1):10-19

Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Objective: To investigate the therapeutic mechanism of resveratrol (RES) for Alzheimer's disease (AD) in light of network pharmacology.

Methods: We searched PubChem, BATMAN-TCM, Genecards, AD, TTD, String 11.0, AlzData, SwissTargetPrediction, Metascape and other databases for the therapeutic targets of RES and human AD-related targets. The intersection was determined using Venny 2.1 to obtain the therapeutic targets of RES for AD. The protein-protein interaction (PPI) network was constructed, the gene ontology (GO) was enriched and the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG pathway) were analyzed. Cytoscape 3.7.1 software was used to construct a target-signaling pathway network of RES in the treatment of AD. Molecular docking verification was carried out on SwissDock (http://www.swissdock.ch/docking). We examined a 293Tau cell model of AD for changes in protein levels of pS396, pS199, Tau5, CDK5, glycogen synthase kinase 3β (GSK3β) and p-GSK3β in response to RES treatment using Western blotting.

Results: We obtained 182 targets of RES, 525 targets related to AD, and 36 targets of RES for AD treatment, among which 34.6% of the targets were protein-modifying enzymes, 27.7% were metabolite invertase, 13.8% were gene-specific transcriptional regulators, and 10.3% were transporters. The core key targets of RES in the treatment of AD included INS, APP, ESR1, MMP9, IGF1R, CACNA1C, MAPT (microtubule- associated protein Tau), MMP2, TGFB1 and GSK3B. Enrichment analysis of GO biological process suggested that the biological function of RES in AD treatment mainly involved the response to β-amyloid protein, positive regulation of transferase activity, the transmembrane receptor protein tyrosine kinase signaling pathway, regulation of behavior, learning or memory, aging, and transmembrane transport. KEGG pathway enrichment analysis showed that the most significantly enriched signaling pathways were AD pathway, PI3K-AKT signaling pathway, cGMP-PKG signaling pathway, and MAPK signaling pathway. Molecular docking results showed that RES had strong binding with ESR1, GSK3B, MMP9, IGF1R, APP and INS. In the cell model of AD, treatment with 50 μmol/L RES for 12 h significantly reduced the levels of pS396 and pS199 by regulating CDK5 and GSK3β activity ( < 0.001).

Conclusions: RES produces therapeutic effects on AD by acting on multiple targets and affecting multiple signaling pathways and improves AD-associated pathologies a direct action on Aβ and Tau pathological processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12122/j.issn.1673-4254.2021.01.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867487PMC
January 2021

The association of continuity of care and risk of mortality in breast cancer patients with cardiometabolic comorbidities.

J Psychosoc Oncol 2021 Jan 18:1-15. Epub 2021 Jan 18.

Harrison School of Pharmacy, Department of Health Outcomes Research and Policy, Auburn University, Auburn, Alabama, USA.

Objective: The association of continuity of care (COC) among providers and mortality risk for breast cancer patients with comorbidities is not sufficiently studied.

Design: A retrospective cohort study using the 2006-2014 Surveillance, Epidemiology and End Results (SEER)-Medicare data.

Participants: Newly diagnosed female breast cancer patients ( = 57,578) with comorbidities (hypertension, hyperlipidemia, and/or diabetes).

Methods: All-cause mortality was assessed annually for up to 5 years. COC was estimated using the Bice-Boxerman index, which included: 1) specialty COC capturing continuity of visits to the same provider type (Primary Care Physicians, Oncologists, and Other specialists) and 2) individual COC capturing continuous care to the same provider regardless of provider specialty. Cox proportional hazards models estimated the hazard ratio (HR) of all-cause mortality across quartile of the COC index.

Results: Mortality was positively associated with advanced tumor stages and number of comorbidities ( < 0.05). Patients with high specialty COC (4th vs. 1st quartile, HR 1.34, 95%CI 1.29-1.40) had higher risks of mortality compared with those with low specialty COC. However, patients with high individual COC (4th vs. 1st quartile, HR 0.53, 95%CI 0.51-0.54) had lower risks of mortality compared to those with low individual COC.

Conclusion: Receiving care from fewer providers is associated with lower mortality and from fewer types of provider is associated with higher mortality. The results might be confounded by uncontrolled factors and provoke the need for alternative patient care models that recognize the balance between appropriate subspecialties and minimizing the fragmentation of care within and across subspecialties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/07347332.2020.1867692DOI Listing
January 2021

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Theranostics 2021 1;11(6):2634-2654. Epub 2021 Jan 1.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Activation of liver X receptor (LXR) by its ligand T0901317 (T317) enhances interferon-γ (IFNγ) production to inhibit tumor growth. However, induction of severe hypertriglyceridemia and fatty liver by T317 limits its application. The naphthylacetic acid modified D-enantiomeric-glycine-phenylalanine-phenylalanine-tyrosine (D-Nap-GFFY) can form a nanofiber hydrogel which is selectively taken up by antigen-presenting cells (APCs). In this study, we determined if D-Nap-GFFY-encapsulated T317 (D-Nap-GFFY-T317) can potently inhibit tumor growth while having no adverse lipogenic effects on the liver. We prepared D-Nap-GFFY-T317 nanofiber hydrogel and subcutaneously injected it into IFNγ deficient (IFNγ) and wild-type (WT) mice with lung carcinoma, either inoculated LLC1 cells or urethane-induced carcinoma. Mice received oral T317 administration were used for comparison. Effects of treatment on tumor growth, lipogenesis and involved mechanisms were investigated. Compared with T317 oral administration, injection of D-Nap-GFFY-T317 more potently inhibited LLC1 tumor growth in mice. The inhibition was dependent on LXR-activated IFNγ expression in APCs. D-Nap-GFFY-T317 increased M1 while reducing M2 type macrophages in tumors. Associated with activation of IFNγ expression, D-Nap-GFFY-T317 enhanced dendritic cell maturation and infiltration into tumors, increased CD3/CD8 cells in tumors, and inhibited tumor angiogenesis. Similarly, D-Nap-GFFY-T317 more potently inhibited growth of urethane-induced lung carcinomas than T317 oral administration. In these two tumor models, T317 oral administration, but not D-Nap-GFFY-T317 injection, activated hepatic lipogenesis and induced fatty liver. Our study demonstrates that D-Nap-GFFY-T317 inhibits lung tumor growth without adverse effects on the liver, indicating the hydrogel-encapsulated LXR ligand might be a novel therapy for tumor treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.53139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806465PMC
January 2021

Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress.

BMC Plant Biol 2021 Jan 9;21(1):36. Epub 2021 Jan 9.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Cyrus Tang Innovation Center for Crop Seed Industry, Nanjing Agricultural University, Nanjing, China.

Background: Rice growth is frequently affected by salinity. When exposed to high salinity, rice seed germination and seedling establishment are significantly inhibited. With the promotion of direct-seeding in Asia, improving rice seed germination under salt stress is crucial for breeding.

Results: In this study, an indica landrace Wujiaozhan (WJZ) was identified with high germinability under salt stress. A BCF population derived from the crossing WJZ/Nip (japonica, Nipponbare)//Nip, was used to quantitative trait loci (QTL) mapping for the seed germination rate (GR) and germination index (GI) under HO and 300 mM NaCl conditions. A total of 13 QTLs were identified, i.e. ten QTLs under HO conditions and nine QTLs under salt conditions. Six QTLs, qGR6.1, qGR8.1, qGR8.2, qGR10.1, qGR10.2 and qGI10.1 were simultaneously identified under two conditions. Under salt conditions, three QTLs, qGR6.2, qGR10.1 and qGR10.2 for GR were identified at different time points during seed germination, which shared the same chromosomal region with qGI6.2, qGI10.1 and qGI10.2 for GI respectively. The qGR6.2 accounted for more than 20% of phenotypic variation under salt stress, as the major effective QTL. Furthermore, qGR6.2 was verified via the BCF population and narrowed to a 65.9-kb region with eleven candidate genes predicted. Based on the microarray database, five candidate genes were found with high transcript abundances at the seed germination stage, of which LOC_Os06g10650 and LOC_Os06g10710 were differentially expressed after seed imbibition. RT-qPCR results showed the expression of LOC_Os06g10650 was significantly up-regulated in two parents with higher levels in WJZ than Nip during seed germination under salt conditions. Taken together, it suggests that LOC_Os06g10650, encoding tyrosine phosphatase family protein, might be the causal candidate gene for qGR6.2.

Conclusions: In this study, we identified 13 QTLs from a landrace WJZ that confer seed germination traits under HO and salt conditions. A major salt-tolerance-specific QTL qGR6.2 was fine mapped to a 65.9-kb region. Our results provide information on the genetic basis of improving rice seed germination under salt stress by marker-assisted selection (MAS).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-020-02820-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797128PMC
January 2021

ERK1/2 inhibition reduces vascular calcification by activating miR-126-3p-DKK1/LRP6 pathway.

Theranostics 2021 1;11(3):1129-1146. Epub 2021 Jan 1.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, Hefei University of Technology, Hefei, China.

: Vascular microcalcification increases the risk of rupture of vulnerable atherosclerotic lesions. Inhibition of ERK1/2 reduces atherosclerosis in animal models while its role in vascular calcification and the underlying mechanisms remains incompletely understood. Levels of activated ERK1/2, DKK1, LRP6 and BMP2 in human calcific aortic valves were determined. ApoE deficient mice received ERK1/2 inhibitor (U0126) treatment, followed by determination of atherosclerosis, calcification and miR-126-3p production. C57BL/6J mice were used to determine the effect of U0126 on Vitamin D (VD)-induced medial arterial calcification. HUVECs, HAECs and HASMCs were used to determine the effects of ERK1/2 inhibitor or siRNA on SMC calcification and the involved mechanisms. : We observed the calcification in human aortic valves was positively correlated to ERK1/2 activity. At cellular and animal levels, U0126 reduced intimal calcification in atherosclerotic lesions of high-fat diet-fed apoE deficient mice, medial arterial calcification in VD-treated C57BL/6J mice, and calcification in cultured SMCs and arterial rings. The reduction of calcification was attributed to ERK1/2 inhibition-reduced expression of ALP, BMP2 and RUNX2 by activating DKK1 and LRP6 expression, and consequently inactivating both canonical and non-canonical Wnt signaling pathways in SMCs. Furthermore, we determined ERK1/2 inhibition activated miR-126-3p production by facilitating its maturation through activation of AMPKα-mediated p53 phosphorylation, and the activated miR-126-3p from ECs and SMCs played a key role in anti-vascular calcification actions of ERK1/2 inhibition. : Our study demonstrates that activation of miR-126-3p production in ECs/SMCs and interactions between ECs and SMCs play an important role in reduction of vascular calcification by ERK1/2 inhibition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.49771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738895PMC
January 2021

[Characteristics and Source Apportionment of Ambient VOCs in Spring in Liuzhou].

Huan Jing Ke Xue 2021 Jan;42(1):65-74

Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China.

The objective was to investigate the characteristics and sources of ambient volatile organic compounds (VOCs) in the karst region in southwestern China. We monitored atmospheric VOCs in Liuzhou by the GC955 VOCs Online Monitoring System and analyzed the pollution characteristics, ozone formation potential (OFP), aerosol formation potential (AFP), and the positive matrix factorization (PMF) model in March 2019. The results show that ① 50 kinds of VOC components were detected during the supervised period, with an average daily concentration of 25.52×10 mol·mol, which was composed of alkanes (56.08%), alkenes (19.63%), alkynes (14.25%), and aromatics (10.04%), respectively. ② The concentration of VOCs was lower during the day and higher at night, with the highest value at 23:00. The VOC concentration was low in daytime and high at night. The peak value of VOCs with regard to diurnal variation was correlated with the time of morning and the evening traffic peak and may be influenced by various factors. ③ The contribution of alkenes, aromatics, and alkanes to OFP was 44.30%, 33.03%, and 19.96%, respectively. This indicates that the control of aromatic and olefin should prioritize alkanes. In addition, Liuzhou city is in the VOC sensitive area of O generation, and the reduction of VOCs had a controlling effect on O generation. ④ The contribution of aromatic hydrocarbons to AFP was up to 95.27%. Therefore, the improvement and control of the processes in motor vehicle exhaust emissions, solvent use, and the automobile industry and the chemical industry could effectively suppress ozone and haze pollution. ⑤ The emission sources of VOCs in spring were mainly industrial emission sources (28.34%), motor vehicle sources (25.47%), combustion sources (24.37%), solvent sources (13.28%), and plant emission sources (8.54%), respectively. This indicates that the control of industrial emission sources, motor vehicle sources, and combustion sources is the main way to control VOC pollution in Liuzhou City. Meanwhile, the olefin and aromatic hydrocarbons emitted by these emission sources should be mainly considered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202004006DOI Listing
January 2021

[Surface water change characteristics of Taihu Lake from 1984-2018 based on Google Earth Engine].

Ying Yong Sheng Tai Xue Bao 2020 Sep;31(9):3163-3172

School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.

Lakes are important fresh water resources. Accurate understanding of lake dynamic changes benefits the sustainable development of water resources and socio-economic development. Based on the Google Earth Engine (GEE), we analyzed the dynamics of Taihu Lake from 1984 to 2018 by adopting the dataset of Joint Research Centre's Global Surface Water and Landsat imagery. The modified normalized difference water index (MNDWI) was used to explore the changes in the area of Taihu Lake in recent 35 years. The results showed that the area of Taihu Lake increased during 1984-2018, with a total increase of 45.31 km. The area in spring and winter was larger than that in summer. Eastern Taihu Lake was the main part of Taihu Lake showing area change. Compared with 1984, 88.9% of the water area of Taihu Lake in 2018 did not change, and 0.3% of the water area disappeared permanently. Lake area changes were affected by both natural and anthropogenic factors. Agricultural irrigation, fish farming, reclamation of surrounding lakes, water conservancy engineering facilities, and the transformation of land-use types caused the reduction in lake area. Increased annual precipitation and the implementation of environmental protection policies were the main factors for the increases in lake area. The results provide reference for effective sustainable water resource management and verify the feasibility of monitoring long-term surface water change based on the GEE platform.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13287/j.1001-9332.202009.011DOI Listing
September 2020

Morphology Tuning and Its Role in Optimization of Perovskite Films Fabricated from A Novel Nonhalide Lead Source.

Adv Sci (Weinh) 2020 Dec 1;7(23):2002296. Epub 2020 Nov 1.

School of Materials and Energy University of Electronic Science and Technology of China Chengdu 611731 P. R. China.

Usage of nonhalide lead sources for fabricating perovskite solar cells (PSCs) has recently attracted increasing attention as a promising route toward realizing high quality PSC devices. However, the unique role of nonhalide lead sources in improving perovskite film morphology and PSC performance has largely remained unexplored, impeding broader application of these materials. Here, it is demonstrated that by using a new nonhalide lead source, lead formate (Pb(HCOO)), good control of perovskite film morphology can be achieved. With the usage of lead formate, PbI can nicely border the perovskite grain boundaries (GBs) and form domain "walls" that segregate the individual perovskite crystal domains. The PbI at the GBs lead to significant improvement in film quality and device performance through passivating the defects at the perovskite GBs and suppressing lateral carrier diffusion. An impressive carrier lifetime at the microsecond scale ( = 1714 ns) is achieved, further with an optimal power conversion efficiency of 20.3% for the resulting devices. This work demonstrates a promising and effective method toward fabricating high-quality perovskites and high-efficiency PSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202002296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709991PMC
December 2020

MEK inhibition reprograms CD8 T lymphocytes into memory stem cells with potent antitumor effects.

Nat Immunol 2021 01 23;22(1):53-66. Epub 2020 Nov 23.

The Loop Immuno-Oncology Laboratory, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.

Regenerative stem cell-like memory (T) CD8 T cells persist longer and produce stronger effector functions. We found that MEK1/2 inhibition (MEKi) induces T that have naive phenotype with self-renewability, enhanced multipotency and proliferative capacity. This is achieved by delaying cell division and enhancing mitochondrial biogenesis and fatty acid oxidation, without affecting T cell receptor-mediated activation. DNA methylation profiling revealed that MEKi-induced T cells exhibited plasticity and loci-specific profiles similar to bona fide T isolated from healthy donors, with intermediate characteristics compared to naive and central memory T cells. Ex vivo, antigenic rechallenge of MEKi-treated CD8 T cells showed stronger recall responses. This strategy generated T cells with higher efficacy for adoptive cell therapy. Moreover, MEKi treatment of tumor-bearing mice also showed strong immune-mediated antitumor effects. In conclusion, we show that MEKi leads to CD8 T cell reprogramming into T that acts as a reservoir for effector T cells with potent therapeutic characteristics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41590-020-00818-9DOI Listing
January 2021

One-Dimensional Multi-Scale Domain Adaptive Network for Bearing-Fault Diagnosis under Varying Working Conditions.

Sensors (Basel) 2020 Oct 23;20(21). Epub 2020 Oct 23.

School of Reliability and Systems Engineering, Beihang University, Beijing 100191, China.

Data-driven bearing-fault diagnosis methods have become a research hotspot recently. These methods have to meet two premises: (1) the distributions of the data to be tested and the training data are the same; (2) there are a large number of high-quality labeled data. However, machines usually work under different working conditions in practice, which challenges these prerequisites due to the fact that the data distributions under different working conditions are different. In this paper, the one-dimensional Multi-Scale Domain Adaptive Network (1D-MSDAN) is proposed to address this issue. The 1D-MSDAN is a kind of deep transfer model, which uses both feature adaptation and classifier adaptation to guide the multi-scale convolutional neural network to perform bearing-fault diagnosis under varying working conditions. Feature adaptation is performed by both multi-scale feature adaptation and multi-level feature adaptation, which helps in finding domain-invariant features by minimizing the distribution discrepancy between different working conditions by using the Multi-kernel Maximum Mean Discrepancy (MK-MMD). Furthermore, classifier adaptation is performed by entropy minimization in the target domain to bridge the source classifier and target classifier to further eliminate domain discrepancy. The Case Western Reserve University (CWRU) bearing database is used to validate the proposed 1D-MSDAN. The experimental results show that the diagnostic accuracy for the 12 transfer tasks performed by 1D-MSDAN was superior to that of the mainstream transfer learning models for bearing-fault diagnosis under variable working conditions. In addition, the transfer learning performance of 1D-MSDAN for multi-target domain adaptation and real industrial scenarios was also verified.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20216039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660602PMC
October 2020

Efficient and High-Luminance Perovskite Light-Emitting Diodes Based on CsPbBr Nanocrystals Synthesized from a Dual-Purpose Organic Lead Source.

Small 2020 Nov 26;16(46):e2003939. Epub 2020 Oct 26.

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu, 611731, P. R. China.

Rational engineering of the surface properties of perovskite nanocrystals (PeNCs) is critical to obtain light emitters with simultaneous high photoluminescence efficiency and excellent charge transport properties for light-emitting diodes (LEDs). However, the commonly used lead halide sources make it hard to rationally optimize the surface compositions of the PeNCs. In addition, previously developed ligand engineering strategies for conventional inorganic nanocrystals easily deteriorate surface properties of the PeNCs, bringing additional difficulties in optimizing their optoelectronic properties. In this work, a novel strategy of employing a dual-purpose organic lead source for the synthesis of highly luminescent PeNCs with enhanced charge transport property is developed. Lead naphthenate (Pb(NA) ), of which the metal ions work as lead sources while the naphthenate can function as the surface ligands afterward, is explored and the obtained products under different synthesis conditions are comprehensively investigated. Monodispersed cesium lead bromide (CsPbBr ) with controllable size and excellent optical properties, showing superior photoluminescence quantum yields up to 80%, is obtained. Based on the simultaneously enhanced electrical properties of the Pb(NA) -derived PeNCs, the resultant LEDs demonstrate a high peak external quantum efficiency of 8.44% and a superior maximum luminance of 31 759 cd cm .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202003939DOI Listing
November 2020

Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression.

J Gastric Cancer 2020 Sep 14;20(3):300-312. Epub 2020 Sep 14.

Medical School, Southeast University, Nanjing, China.

Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556.

Materials And Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 () in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer.

Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice.

Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/ axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5230/jgc.2020.20.e28DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521983PMC
September 2020

Automatic Recognition of Sucker-Rod Pumping System Working Conditions Using Dynamometer Cards with Transfer Learning and SVM.

Sensors (Basel) 2020 Oct 3;20(19). Epub 2020 Oct 3.

Department of Computer Science, Electrical and Space Engineering, Luleå University of Technology, 97187 Luleå, Sweden.

Sucker-rod pumping systems are the most widely applied artificial lift equipment in the oil and gas industry. Accurate and intelligent working condition recognition of pumping systems imposes major impacts on oilfield production benefits and efficiency. The shape of dynamometer card reflects the working conditions of sucker-rod pumping systems, and different conditions can be indicated by their typical card characteristics. In traditional identification methods, however, features are manually extracted based on specialist experience and domain knowledge. In this paper, an automatic fault diagnosis method is proposed to recognize the working conditions of sucker-rod pumping systems with massive dynamometer card data collected by sensors. Firstly, AlexNet-based transfer learning is adopted to automatically extract representative features from various dynamometer cards. Secondly, with the extracted features, error-correcting output codes model-based SVM is designed to identify the working conditions and improve the fault diagnosis accuracy and efficiency. The proposed AlexNet-SVM algorithm is validated against a real dataset from an oilfield. The results reveal that the proposed method reduces the need for human labor and improves the recognition accuracy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20195659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582724PMC
October 2020

TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells.

J Biol Chem 2020 11 22;295(48):16314-16327. Epub 2020 Sep 22.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China. Electronic address:

TNF ligand-related molecule 1A (TL1A) is a vascular endothelial growth inhibitor to reduce neovascularization. Lack of apoE a expression results in hypercholesterolemia and atherosclerosis. In this study, we determined the precise effects of TL1A on the development of atherosclerosis and the underlying mechanisms in apoE-deficient mice. After 12 weeks of pro-atherogenic high-fat diet feeding and TL1A treatment, mouse aorta, serum, and liver samples were collected and used to assess atherosclerotic lesions, fatty liver, and expression of related molecules. We found that TL1A treatment significantly reduced lesions and enhanced plaque stability. Mechanistically, TL1A inhibited formation of foam cells derived from vascular smooth muscle cells (VSMCs) but not macrophages by activating expression of ABC transporter A1 (ABCA1), ABCG1, and cholesterol efflux in a liver X receptor-dependent manner. TL1A reduced the transformation of VSMCs from contractile phenotype into synthetic phenotypes by activating expression of contractile marker α smooth muscle actin and inhibiting expression of synthetic marker osteopontin, or osteoblast-like phenotype by reducing calcification. In addition, TL1A ameliorated high-fat diet-induced lipid metabolic disorders in the liver. Taken together, our work shows that TL1A can inhibit the development of atherosclerosis by regulating VSMC/foam cell formation and switch of VSMC phenotypes and suggests further investigation of its potential for atherosclerosis treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.RA120.015486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705304PMC
November 2020

Adiponectin agonist ADP355 ameliorates doxorubicin-induced cardiotoxicity by decreasing cardiomyocyte apoptosis and oxidative stress.

Biochem Biophys Res Commun 2020 12 18;533(3):304-312. Epub 2020 Sep 18.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China. Electronic address:

Doxorubicin (DOX) is an anthracycline derivative and widely used as an anticancer drug. However, the severe cardiotoxicity of DOX limits its application. ADP355 is an adiponectin-based active peptide with anti-liver fibrosis and atherosclerosis properties. It remains unclear the effects and involved mechanisms of ADP355 in DOX-induced cardiotoxicity. C57BL/6J mice were intraperitoneally injected DOX once a week to induce heart failure while receiving ADP355 treatment daily for 4 weeks. At the end of experiment, blood and heart tissues were collected. We found that ADP355 markedly improved DOX-induced cardiac dysfunction and histopathological damage, and decreased serum creatine kinase, lactate dehydrogenase and hydroxybutyrate dehydrogenase levels. The anti-apoptotic activity of ADP355 was indicated by reduction in TUNEL-positive cells and cleaved caspase-3 expression, along with decreased BCL2-associated X protein/B cell lymphoma 2 (BAX/BCL2) levels in heart tissues. Additionally, ADP355 markedly increased DOX-decreased cell viability by reducing BAX/BCL2, but inhibited reactive oxygen species production in H9c2 cells. Mechanistically, ADP355 attenuated expression of DOX-reduced nuclear factor-erythroid 2-related factor 2 (Nrf2) and superoxide dismutase 2, as well as mRNA levels of Nrf2 downstream targets. Furthermore, ADP355 activated sirtuin 2 and its target genes. In conclusion, we demonstrate that ADP355 alleviates DOX-induced cardiotoxicity by inhibiting myocardial apoptosis and oxidative stress through Nrf2 and sirtuin 2 signaling pathways. These findings suggest that ADP355 can be a promising candidate for the treatment of cardiac dysfunction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2020.09.035DOI Listing
December 2020

Ponatinib and other CML Tyrosine Kinase Inhibitors in Thrombosis.

Int J Mol Sci 2020 Sep 8;21(18). Epub 2020 Sep 8.

Departments of Medicine and Pathology, Case Western Reserve University and University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA.

Abl1 kinase has important biological roles. The Bcr-Abl1 fusion protein creates undesired kinase activity and is pathogenic in 95% of chronic myeloid leukemia (CML) and 30% of acute lymphoblastic leukemia (ALL) patients. Targeted therapies to these diseases are tyrosine kinase inhibitors. The extent of a tyrosine kinase inhibitor's targets determines the degree of biologic effects of the agent that may influence the well-being of the patient. This fact is especially true with tyrosine kinase inhibitor effects on the cardiovascular system. Thirty-one percent of ponatinib-treated patients, the tyrosine kinase inhibitor with the broadest inhibitory spectrum, have thrombosis associated with its use. Recent experimental investigations have indicated the mechanisms of ponatinib-associated thrombosis. Further, an antidote to ponatinib is in development by re-purposing an FDA-approved medication.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21186556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555546PMC
September 2020

Optical coherence tomography angiography assessment of 577 nm laser effect on severe non-proliferative diabetic retinopathy with diabetic macular edema.

Int J Ophthalmol 2020 18;13(8):1257-1265. Epub 2020 Aug 18.

Department of Ophthalmology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China.

Aim: To quantitatively evaluate the effect of the combined use of 577-nm subthreshold micropulse macular laser (SML) and multi-point mode pan retinal laser photocoagulation (PRP) on severe non-proliferative diabetic retinopathy (NPDR) with central-involved diabetic macular edema (CIDME) using optical coherence tomography angiography (OCTA).

Methods: In this observational clinical study, 86 eyes of 86 NPDR patients with CIDME who underwent SML and PRP treatment were included. Images were obtained 1d before laser and post-laser (1d, 1wk, 1, 3, and 6mo) using AngioVue software 2.0. Best corrected visual acuity (BCVA, LogMAR), foveal avascular zone area (FAZ), choriocapillary flow area (ChF), parafoveal vessel density (PVD), capillary density inside disc (CDD), peripapillary capillary density (PCD), macular ganglion cell complex thickness (mGCCT), central macular thickness (CMT), and subfoveal choroidal thickness (ChT) were compared between pre- and post-laser treatment.

Results: BCVA remained stable during 6mo post-laser therapy (pre-laser 6mo post-laser: 0.53±0.21 0.5±0.15, >0.05). PVD, ChF, ChT, CMT, and mGCCT significantly increased 1d post-laser therapy [pre-laser 1d post-laser: superficial PVD (%), 40.51±3.42 42.43±4.68; deep PVD (%), 42.66±3.67 44.78±4.52; ChF, 1.72±0.21 1.9±0.12 mm; ChT, 302.45±69.74 319.38±70.93 µm; CMT, 301.65±110.78 320.86±105.62 µm; mGCCT, 105.71±10.72 115.46±9.64 µm; <0.05]. However, PVD, ChF and ChT decreased to less than baseline level at 6mo post-laser therapy (pre-laser 6mo post-laser: superficial PVD (%), 40.51±3.42 36.32±4.19; deep PVD (%), 42.66±3.67 38.76±3.74; ChF, 1.72±0.21 1.62±0.09 mm; ChT, 302.45±69.74 289.61±67.55 µm; <0.05), whereas CMT and mGCCT decreased to baseline level at 6mo post-laser therapy (CMT, 301.65±110.78 297.77±90.23 µm; mGCCT, 105.71±10.72 107.05±11.81 µm; >0.05). Moreover, FAZ continuously increased while CDD and PCD continuously decreased in 6mo after laser therapy. CMT and ChT had a significant positive correlation with ChF and PVD in most post-laser stages.

Conclusion: During a 6-month follow-up period after combined use of SML and PRP therapy, BCVA remained stable and there was a decreased trend in macular edema. Blood flow increased at 1d post-laser therapy and reduced at 6mo post-laser therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18240/ijo.2020.08.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387903PMC
August 2020

OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY FINDINGS OF MICROVASCULAR AND NEURAL CHANGES IN PRIMARY PULMONARY HYPERTENSION.

Retina 2021 Apr;41(4):784-792

Department of Ophthalmology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China; and.

Purpose: To investigate the microvascular and neural changes in primary pulmonary hypertension (PPH) patients compared with healthy controls.

Methods: Forty-four eyes of 22 PPH patients were included in this observational clinical cohort study, and 44 eyes of 22 healthy participants were enrolled as controls. Optical coherence tomography angiography images were obtained from each participant using the RTVue XR Avanti device with AngioVue software 2.0.

Results: Regarding the total macular-associated vessel density, including that of the superficial and deep retina, the optic disk-associated capillary density, including that of the whole image, capillary density inside the disk, and the peripapillary region, was significantly lower in the PPH group than in the control group. There was a similar trend in the retinal nerve fiber layer thickness and the ganglion cell complex thickness, whereas the focal loss volume and the global loss volume were greater in the PPH group than the control group.

Conclusion: Changes in the capillary density and thickness of the retina and the optic nerve head in PPH patients can be detected by optical coherence tomography angiography. Parameters including the macular-associated vessel density, optic disk-associated capillary density, retinal nerve fiber layer, ganglion cell complex, focal loss volume, and global loss volume may provide useful evidence for the early detection of microvascular and neural impairments in patients with PPH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/IAE.0000000000002940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989611PMC
April 2021

Reduced Nogo expression inhibits diet-induced metabolic disorders by regulating ChREBP and insulin activity.

J Hepatol 2020 12 29;73(6):1482-1495. Epub 2020 Jul 29.

Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China. Electronic address:

Background & Aims: Chronic overconsumption of a high-carbohydrate diet leads to steatosis and its associated metabolic disorder and, eventually, to non-alcoholic fatty liver disease. Carbohydrate-responsive element binding protein (ChREBP) and insulin regulate de novo lipogenesis from glucose. Herein, we studied the effect of reticulon-4 (Nogo) expression on diet-induced metabolic disorders in mice.

Methods: Nogo-deficient (Nogo) and littermate control [wild-type (WT)] mice were fed a high-glucose or high-fructose diet (HGD/HFrD) to induce metabolic disorders. The effects of Nogo small interfering (si) RNA (siRNA) on HFrD-induced metabolic disorders were investigated in C57BL/6J mice.

Results: HGD/HFrD induced steatosis and its associated metabolic disorders in WT mice by activating ChREBP and impairing insulin sensitivity. They also activated Nogo-B expression, which in turn inhibited insulin activity. In response to HGD/HFrD feeding, Nogo deficiency enhanced insulin sensitivity and energy metabolism to reduce the expression of ChREBP and lipogenic molecules, activated AMP-activated catalytic subunit α, peroxisome proliferator activated receptor α and fibroblast growth factor 21, and reduced endoplasmic reticulum (ER) stress and inflammation, thereby blocking HGD/HFrD-induced hepatic lipid accumulation, insulin resistance and other metabolic disorders. Injection of Nogo siRNA protected C57BL/6J mice against HFrD-induced metabolic disorders by ameliorating insulin sensitivity, ChREBP activity, ER stress and inflammation.

Conclusions: Our study identified Nogo as an important mediator of insulin sensitivity and ChREBP activity. Reduction of Nogo expression is a potential strategy for the treatment of high-carbohydrate diet-induced metabolic complications.

Lay Summary: Nogo deficiency blocks high-carbohydrate diet-induced glucose intolerance and insulin resistance, while increasing glucose/lipid utilisation and energy expenditure. Thus, reduction of Nogo expression protects against high-carbohydrate diet-induced body-weight gain, hepatic lipid accumulation and the associated metabolic disorders, indicating that approaches inhibiting Nogo expression can be applied for the treatment of diseases associated with metabolic disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhep.2020.07.034DOI Listing
December 2020

Physiological responses, tolerance efficiency, and phytoextraction potential of (Boreau) H. Ohba under Cd stress in hydroponic condition.

Int J Phytoremediation 2021 29;23(1):80-88. Epub 2020 Jul 29.

School of Metallurgy and Environment, Central South University, Changsha, China.

A sand hydroponic experiment with different concentrations of 0, 5, 10, 20, 40 mg L Cd was used to study the growth and physiological response of (Boreau) H. Ohba. and its phytoextraction potential for Cd. The results showed that total plant biomass under 5 mg L Cd treatment was slightly affected. The content of malondialdehyde (MDA) in leaf exposed to Cd was higher, and the POD and CAT activity exhibited a positive response to the low level of Cd addition (5 mg·L). The photosynthesis pigments were slightly inhibited, and the ultrastructure of chloroplast remained intact after treatment with 10 mg L Cd. The maximum leaf Cd content (603 mg·kg) was found in 5 mg L Cd treatment, then decreased with the Cd level increased. The maximum Cd content in the shoots far exceeds the threshold level (100 mg kg) for a Cd-hyperaccumulator plant with the value of translocation factor (TF) for Cd reaching up to 5.62. In conclusion, showed normal growth and physiological response and high shoot Cd accumulation under 5 mg L Cd stress, which made it to be a good candidate for phytoextraction of low-level Cd polluted environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15226514.2020.1797628DOI Listing
January 2021