Publications by authors named "Peng Yuan"

730 Publications

Rational design and fabrication of a novel acid-resistant UZM-5 zeolite membrane for pervaporation dehydration processes.

Chem Commun (Camb) 2021 Sep 21;57(75):9574-9577. Epub 2021 Sep 21.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.

Novel thin UZM-5 zeolite membranes with pure UFI phase were successfully fabricated using a charge density mismatch-assisted tertiary growth approach. Taking full advantage of the suitable Si/Al ratio and pore size, the obtained novel zeolite membranes revealed an outstanding acid-resistant capacity and provided great potential for acetic acid pervaporation dehydration applications.
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http://dx.doi.org/10.1039/d1cc03987cDOI Listing
September 2021

Towards the Representation of Genomic Data in HL7 FHIR and OMOP CDM.

Stud Health Technol Inform 2021 Sep;283:86-94

Institute for Medical Informatics and Biometry at Carl Gustav Carus Faculty of Medicine at Technische Universität Dresden, Germany.

High throughput sequencing technologies have facilitated an outburst in biological knowledge over the past decades and thus enables improvements in personalized medicine. In order to support (international) medical research with the combination of genomic and clinical patient data, a standardization and harmonization of these data sources is highly desirable. To support this increasing importance of genomic data, we have created semantic mapping from raw genomic data to both FHIR (Fast Healthcare Interoperability Resources) and OMOP (Observational Medical Outcomes Partnership) CDM (Common Data Model) and analyzed the data coverage of both models. For this, we calculated the mapping score for different data categories and the relative data coverage in both FHIR and OMOP CDM. Our results show, that the patients genomic data can be mapped to OMOP CDM directly from VCF (Variant Call Format) file with a coverage of slightly over 50%. However, using FHIR as intermediate representation does not lead to further information loss as the already stored data in FHIR can be further transformed into OMOP CDM format with almost 100% success. Our findings are in favor of extending OMOP CDM with patient genomic data using ETL to enable the researchers to apply different analysis methods including machine learning algorithms on genomic data.
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http://dx.doi.org/10.3233/SHTI210545DOI Listing
September 2021

The Safety and Effectiveness of Bevacizumab in the Treatment of Nonsquamous Non-Small-Cell Lung Cancer: A Meta-Analysis of Randomized Controlled Trials.

Biomed Res Int 2021 7;2021:5537899. Epub 2021 Sep 7.

Department of Pharmacy, Affiliated Hospital of North Sichuan Medical College, China.

Objective: Bevacizumab was currently available for nonsquamous non-small-cell lung cancer (NSqNSCLC) patients and has been studied in several randomized controlled trials (RCTs) for treatment of these patients. This meta-analysis summarizes the most up-to-date evidences regarding the effects and adverse reactions of bevacizumab in the treatment of NSqNSCLC patients.

Methods: The authors searched for RCTs from electronic database including PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials. Experimental arm was defined as the bevacizumab-containing group and the control arm as the bevacizumab-free group. Data of objective response rate (ORR), disease control rate (DCR), progression-free survival (PFS), overall survival (OS), and adverse reactions were synthetically extracted. A protocol for this meta-analysis has been registered on PROSPERO (http://www.crd.york.ac.uk/prospero).

Results: Ten RCTs that involved a total of 3134 patients were included. The experimental group was associated with significant superior ORR (RR 1.63, 95% CI 1.24 to 2.14, < 0.001), OS (HR 0.90, 95% CI 0.82 to 0.99, < 0.001), and prolonged PFS (HR 0.68, 95% CI 0.62 to 0.74, < 0.001) compared to the control. No significant difference was observed regarding DCR (RR 1.13, 95% CI 0.99 to 1.30, = 0.08). The experimental group showed higher rate of hypertension (RR 6.91, 95% CI 4.62 to 10.35, < 0.00001) and hemorrhagic events (RR 3.07, 95% CI 1.78 to 5.30, < 0.0001) than the control group. The experimental group showed lower rate of anemia (RR 0.72, 95% CI 0.55 to 0.96, = 0.02) than the control group. No significant difference was observed regarding treatment-related adverse event grade 3-5 (TRAE3-5) (RR 1.23, 95% CI 0.99 to 1.53, = 0.06), thrombocytopenia (RR 1.11, 95% CI 0.92 to 1.33, = 0.29), and neutropenia (RR 1.11, 95% CI 0.88 to 1.40, = 0.36).

Conclusion: This meta-analysis showed that bevacizumab could increase ORR, OS, and prolonged PFS for treatment of NSqNSCLC patients. However, no significant improvement in DCR was observed and bevacizumab could increase the rate of hypertension and hemorrhagic events. Bevacizumab was an acceptable option for NSqNSCLC patients. This trial is registered with : CRD42021226790.
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http://dx.doi.org/10.1155/2021/5537899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440089PMC
September 2021

ERRATUM: Effect of Scutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action.

Am J Chin Med 2021 Sep 16:1-2. Epub 2021 Sep 16.

Clinical and Health Sciences, University of South Australia, Adelaide 5000, South Australia, Australia.

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http://dx.doi.org/10.1142/S0192415X21920014DOI Listing
September 2021

Corrigendum to "Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells" [Canc. Lett. 469 (2020) 498-509].

Cancer Lett 2021 Sep 11. Epub 2021 Sep 11.

State Key Laboratory of Cancer Biology and Experimental Teaching Center of Basic Medicine, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. Electronic address:

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http://dx.doi.org/10.1016/j.canlet.2021.09.006DOI Listing
September 2021

Evolutionary rewiring of the wheat transcriptional regulatory network by lineage-specific transposable elements.

Genome Res 2021 Sep 9. Epub 2021 Sep 9.

Institude of Plant Physiology and Ecology;

More than 80% of the wheat genome consists of transposable elements (TEs), which act as one major driver of wheat genome evolution. However, their contributions to the regulatory evolution of wheat adaptations remain largely unclear. Here, we created genome-binding maps for 53 transcription factors (TFs) underlying environmental responses by leveraging DAP-seq in , together with epigenomic profiles. Most TF-binding sites (TFBS) located distally from genes are embedded in TEs, whose functional relevance is supported by purifying selection and active epigenomic features. About 24% of the non-TE TFBS share significantly high sequence similarity with TE-embedded TFBS. These non-TE TFBS have almost no homologous sequences in non-Triticeae species and are potentially derived from Triticeae-specific TEs. The expansion of TE-derived TFBS linked to wheat-specific gene responses, suggesting TEs are an important driving force for regulatory innovations. Altogether, TEs have been significantly and continuously shaping regulatory networks related to wheat genome evolution and adaptation.
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http://dx.doi.org/10.1101/gr.275658.121DOI Listing
September 2021

Graphene oxide promoted chromium uptake by zebrafish embryos with multiple effects: Adsorption, bioenergetic flux and metabolism.

Sci Total Environ 2021 Aug 26;802:149914. Epub 2021 Aug 26.

School of Public Health, Xinxiang Medical University, Xinxiang 453003, China.

The increasing production and application of graphene oxide (GO, a popular carbon nanomaterial), makes their release into aqueous environment inevitably. The capability of GO to enhance the toxicity of background contaminants has been widely concerned. However, the effect of GO on heavy metal accumulation in fish embryos remains unclear. Here, we show that GO-promoted chromium (Cr) uptake by zebrafish embryos with multiple effects. The adsorption accelerated the aggregation and settlement of Cr-adsorbed GO and decreased the Cr concentration in the upper water, which enhanced the interaction of chorions and contaminants (Cr, GO and Cr-adsorbed GO). In the presence of GO, the Cr content in chorions and intra-chorion embryos was increased by four times and 57% respectively, compared to that of the single Cr exposure. Furthermore, GO+Cr increased the oxygen consumption rates, embryonic acid extrusion rates and ATP production, induced more serious oxidative stress, and disturbed amino acid metabolism, fatty acid metabolism and TCA cycle. These findings provide new insights into the effect of GO on heavy metal bioaccumulation and toxicity during embryogenesis.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149914DOI Listing
August 2021

miR‑32‑5p suppresses the proliferation and migration of pancreatic adenocarcinoma cells by targeting TLDC1.

Mol Med Rep 2021 11 1;24(5). Epub 2021 Sep 1.

Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.

Pancreatic adenocarcinoma (PAAD) is one of the most fatal types of cancer in humans. However, the molecular mechanisms underlying the migration and invasion abilities of PAAD cells remain unclear. The aim of the present study was to explore the regulatory roles of microRNA (miR)‑32‑5p in PAAD cells. miR‑32‑5p mimic and inhibitor were used to transfect the human PAAD AsPC‑1 cell line to determine the role of miR‑32‑5p in cell proliferation and metastasis. The starBase database predicted the binding of miR‑32‑5p to the target gene TBC/LysM‑associated domain containing 1 (TLDC1). Further analyses were performed to assess miR‑32‑5p and TLDC1 expression levels in healthy and PAAD tissues, as well as the association between miR‑32‑5p or TLDC1 expression and the prognosis of patients with PAAD. The interaction between miR‑32‑5p and TLDC1 was verified using the dual‑luciferase reporter assay. miR‑32‑5p and TLDC1 expression levels were detected by reverse transcription‑quantitative PCR and western blotting, respectively. The Cell Counting Kit‑8 assay was utilised to assess cell proliferation, whereas the wound‑healing and Transwell assays were conducted to assess cell migration and invasion, respectively. miR‑32‑5p expression levels were markedly lower in PAAD tissue compared with those in healthy tissue, and were significantly lower in PAAD cell lines compared with those in the human pancreatic duct cell line HPDE6, which corresponded with poor prognosis. miR‑32‑5p significantly inhibited the proliferation of PAAD cells and markedly reduced migration and invasion compared with the negative controls. miR‑32‑5p was shown to target TLDC1, with miR‑32‑5p expression in PAAD being negatively correlated with TLDC1 expression. High TLDC1 expression levels were associated with a poorer prognosis compared with low TLDC1 expression levels. Co‑transfection of miR‑32‑5p mimic and pcDNA/TLDC1 demonstrated that TLDC1 significantly reversed miR‑32‑5p‑mediated inhibition of the proliferation, migration and invasion of PAAD cells. Overall, the present study demonstrated that miR‑32‑5p may serve as a tumor‑suppressor gene by inhibiting the proliferation and migration and invasion of PAAD cells via the downregulation of TLDC1. Therefore, miR‑32‑5p may serve as a potential diagnostic or prognostic marker for PAAD.
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http://dx.doi.org/10.3892/mmr.2021.12392DOI Listing
November 2021

Evaluation of whether adjuvant chemotherapy can be safely omitted for older female patients with ER-positive, HER2-negative N1 breast cancer: a study based on the SEER database.

Ann Transl Med 2021 Jul;9(13):1082

Department of Breast Disease, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Background: This study evaluated the trends and practice patterns associated with adjuvant chemotherapy (CT) use for patients aged ≥70 years with estrogen receptor-positive (ER+), human epidermal growth factor receptor 2-negative (HER2-) N1 (1-3 positive lymph nodes) breast cancer (BC). Furthermore, the relationship between adjuvant CT and survival in this set of patients was determined.

Methods: The Surveillance, Epidemiology, and End Results (SEER) database was used to identify 6,711 women with ER+, HER2- N1 BC who were aged ≥70 years between 2010 and 2015. Demographic, clinical, and pathological predictors of CT use were identified using logistic regression. Multivariable Cox regression was used to identify variables that correlated with overall survival (OS), before and after propensity score matching (PSM).

Results: Younger age at diagnosis, other histological types, higher tumor grade, larger tumor size, breast reconstruction surgery, progesterone receptor-negative (PR-), and increased nodal involvement were associated with an increased probability of receiving CT. CT use was associated with improved 5-year OS, both before and after PSM [hazard ratio (HR): 0.66, 95% confidence interval (CI): 0.58-0.75 and HR: 0.81, 95% CI: 0.68-0.96, respectively]. The exploratory subgroup analysis showed that although the benefit of CT was significant in the grade III subgroup, it was not significant in the grades I-II subgroups.

Conclusions: Adjuvant CT improved 5-year OS in patients with ER+, HER2- N1 BC who were aged ≥70 years; however, the benefit of CT was more significant in the grade III subgroup than in the grades I-II subgroups.
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http://dx.doi.org/10.21037/atm-21-3097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339850PMC
July 2021

Pyrotinib Plus Vinorelbine Lapatinib Plus Capecitabine in Patients With Previously Treated HER2-Positive Metastatic Breast Cancer: A Multicenter, Retrospective Study.

Front Oncol 2021 5;11:699333. Epub 2021 Aug 5.

Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: Pyrotinib is a newly-developed irreversible pan-ErbB (erythroblastic leukemia viral oncogene homolog) receptor oral tyrosine kinase inhibitor (TKI) with promising efficacy in the human epidermal growth factor receptor-2 (HER2) positive breast cancer. The phase III PHOEBE study proved that pyrotinib plus capecitabine exceeded lapatinib plus capecitabine (LX) in PFS (p < 0.001). Oral vinorelbine is commonly used in combination with anti-HER2 treatment. However, no evidence was reported in terms of the real-world pattern, safety, and efficacy of pyrotinib plus vinorelbine (NP) compared with LX.

Methods: Medical records were retrospectively evaluated for all HER2-positive metastatic breast cancer (MBC) patients who experienced progression on prior trastuzumab-containing regimens (advanced setting) and taxane (any setting) and received NP or LX therapy from 2015 to 2021 in five institutions.

Results: A total of 224 patients were enrolled and evaluated, of which 132 (58.9%) patients received LX and 92 (41.1%) patients received NP. The median progression-free survival (mPFS) of NP group was significantly longer than that in LX group (8.3 5.0 months, HR = 0.47 95% CI 0.34-0.65, p < 0.001). The advantage of NP over LX was seen both in patients with trastuzumab resistance (p < 0.001) and refractoriness (p = 0.004). The NP group had more diarrhea cases (23.9%) compared to the LX group (8.3%). Discontinuation rates in the two groups were similar.

Conclusions: This trial revealed the clinical practice of NP and LX treatment among HER2+ MBC patients pretreated with trastuzumab in China. More patients received LX than NP in real-world while the efficacy of NP exceeded LX in terms of PFS regardless of resistant status of trastuzumab. Although the NP group had more diarrhea cases, toxicities in both groups were acceptable.
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http://dx.doi.org/10.3389/fonc.2021.699333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374067PMC
August 2021

Endovascular repair for retrograde type A intramural hematoma with focal intimal disruption in descending aorta.

J Thorac Dis 2021 Jul;13(7):4250-4259

Department of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, China.

Background: This study aims to report the experience of a single center using thoracic endovascular aortic repair (TEVAR) to treat retrograde type A intramural hematoma (IMH) with focal intimal disruption (FID) in descending aorta.

Methods: A total of 24 consecutive patients with retrograde type A IMH and complicated with FID in descending aorta underwent TEVAR in our center from 2015 to 2020. Their clinical data, imaging manifestation and follow-up results were retrospectively reviewed and analyzed.

Results: The median age of patients was 57.9 years (range, 42-80 years) and 18 were men (75%). As the preoperative CT angiography showed, the 24 patients developed IMH complicated with different kinds of FIDs in descending aorta [5 had intramural blood pool (IBP), 15 had ulcer-like projection (ULP), 2 had penetrating atherosclerotic ulcer (PAU), and 5 had localized dissection]. Successful deployment of aortic stent graft was achieved in all patients. There was no endoleak, stent graft migration, spinal cord ischemia, stroke, or 30-day mortality observed after TEVAR. The median duration of follow-up was 30.0 months (range, 3-60 months). As the last follow-up CT angiography showed, most of the patients (23 in 24, 96%) had favorable aortic remodeling. The maximum hematoma thicknesses and maximum diameters of both ascending and descending aorta were significantly decreased. During follow-up, 1 patient developed retrograde type A aortic dissection (RAAD) and underwent open surgery 3 months after TEVAR. 1 patient died of lung cancer 2 years later. There was no aorta-related death observed.

Conclusions: TEVAR provides a safe and effective treatment strategy for selected patients with retrograde type A IMH, and FID developed in descending aorta could be the possible treatment target. However, RAAD remains one of the most serious postoperative complications of concern.
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http://dx.doi.org/10.21037/jtd-21-574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339735PMC
July 2021

Inhibition of METTL3/m6A/miR126 promotes the migration and invasion of endometrial stromal cells in endometriosis.

Biol Reprod 2021 Aug 12. Epub 2021 Aug 12.

Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, China.

N6-methyladenosine (m6A), one of the most abundant RNA modifications, is involved in the progression of many diseases, but its role and related molecular mechanisms in endometriosis remain unknown. To address these issues, we detected m6A levels in normal, eutopic and ectopic endometrium and found the m6A levels decreased in eutopic and ectopic endometrium compared with normal endometrium. In addition, we proved that methyltransferase-like 3 (METTL3) downregulation accounted for m6A reduction in endometriosis. Furthermore, we observed that METTL3 knockdown facilitated the migration and invasion of human endometrial stromal cells (HESCs), while METTL3 overexpression exerted opposite effects, suggesting that METTL3 downregulation might contribute to endometriosis development by enhancing cellular migration and invasion. Mechanistically, METTL3-dependent m6A was involved in the DGCR8-mediated maturation of primary microRNA126 (miR126, pri-miR126). Moreover, miR126 inhibitor significantly enhanced the migration and invasion of METTL3-overexpressing HESCs, whereas miR126 mimics attenuated the migration and invasion of METTL3-silenced HESCs. Our study revealed the METTL3/m6A/miR126 pathway, whose inhibition might contribute to endometriosis development by enhancing cellular migration and invasion. It also showed that METTL3 might be a novel diagnostic biomarker and therapeutic target for endometriosis.
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http://dx.doi.org/10.1093/biolre/ioab152DOI Listing
August 2021

Successful management of lung adenocarcinoma with ALK/EGFR co-alterations and PD-L1 over-expression by bevacizumab combined with chemotherapy.

Angiogenesis 2021 Aug 3. Epub 2021 Aug 3.

Department of Cancer Center, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China.

Anaplastic lymphoma kinase (ALK)/epidermal growth factor receptor (EGFR) co-alterations in adenocarcinomas are rare and no therapeutic consensus is reached. The potentially negative prognostic effects of programmed death-ligand 1 (PD-L1) expression on tyrosine kinase inhibitor (TKIs) efficacy further complicates the treatment options for patients with ALK/EGFR co-alterations and PD-L1 over-expression. We describe a case of advanced lung adenocarcinoma, harboring concurrent ALK/EGFR mutations and extremely high PD-L1 expression, that achieved sustained remission by the first-line treatment strategy of antiangiogenic therapy combined with chemotherapy. It is our firm conviction that the use anti-angiogenics should not have fallen out of favor in this era of targeted therapy and checkpoint inhibitors.
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http://dx.doi.org/10.1007/s10456-021-09811-8DOI Listing
August 2021

Exploring the role of autophagy during early human embryonic development through single-cell transcriptome and methylome analyses.

Sci China Life Sci 2021 Jul 22. Epub 2021 Jul 22.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.

Early human embryogenesis is a very sophisticated process due to its unique gene regulatory network. Autophagy has been suggested to play an important role in mediating the development of early embryonic cells in mammals. However, evidence showing how autophagy regulates early human embryogenesis remains to be further explored. In this study, we systematically investigated the human transcriptome and methylome patterns of autophagy-related (ATG) genes in early embryonic cells at single-cell resolution. We analyzed the transcriptomic data of 365 cells and methylome data of 265 cells. The results showed that most ATG genes remained epigenetically active and were expressed stably throughout early embryogenesis, whereas the dynamics varied among different developmental stages. This evidence indicated that the autophagy pathway was constitutively activated and exerted a fundamental role in early human embryo development. Our work, for the first time, comprehensively reveals the features of autophagy during early human embryo development.
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http://dx.doi.org/10.1007/s11427-021-1948-1DOI Listing
July 2021

Optimizing Green-Gray Infrastructure for Non-Point Source Pollution Control under Future Uncertainties.

Int J Environ Res Public Health 2021 07 16;18(14). Epub 2021 Jul 16.

School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China.

Non-Point Source Pollution (NPS) caused by polluted and untreated stormwater runoff discharging into water bodies has become a serious threat to the ecological environment. Green infrastructure and gray infrastructure are considered to be the main stormwater management measures, and the issue of their cost-effectiveness is a widespread concern for decision makers. Multi-objective optimization is one of the most reliable and commonly used approaches in solving cost-effectiveness issues. However, many studies optimized green and gray infrastructure under an invariant condition, and the additional benefits of green infrastructure were neglected. In this study, a simulation-optimization framework was developed by integrated Stormwater Management Model (SWMM) and Non-dominated Sorting Genetic Algorithm (NSGA-II) to optimize green and gray infrastructure for NPS control under future scenarios, and a realistic area of Sponge City in Nanchang, China, was used as a typical case. Different levels of additional benefits of green infrastructure were estimated in the optimizing process. The results demonstrated that green-gray infrastructure can produce a co-benefit if the green infrastructure have appropriate Value of Additional Benefits (VAB), otherwise, gray infrastructure will be a more cost-effectiveness measure. Moreover, gray infrastructure is more sensitive than green infrastructure and green-gray infrastructure under future scenarios. The findings of the study could help decision makers to develop suitable planning for NPS control based on investment cost and water quality objectives.
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http://dx.doi.org/10.3390/ijerph18147586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303129PMC
July 2021

Single-Phase Covalent Organic Framework Staggered Stacking Nanosheet Membrane for CO -Selective Separation.

Angew Chem Int Ed Engl 2021 Aug 20;60(35):19047-19052. Epub 2021 Jul 20.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.

Two-dimensional covalent organic frameworks (2D COFs) are considered as potential candidates for gas separation membranes, benefiting from permanent porosity, light-weight skeletons, excellent stability and facilely-tailored functionalities. However, their pore sizes are generally larger than the kinetic diameters of common gas molecules. One great challenge is the fabrication of single-phase COF membranes to realize precise gas separations. Herein, three kinds of high-quality β-ketoenamine-type COF nanosheets with different pore sizes were developed and aggregated to ultrathin nanosheet membranes with distinctive staggered stacking patterns. The narrowed pore sizes derived from the micro-structures and selective adsorption capacities synergistically endowed the COF membranes with intriguing CO -philic separation performances, among which TpPa-2 with medium pore size exhibited an optimal CO /H separation factor of 22 and a CO permeance of 328 gas permeation units at 298 K. This membrane performance reached the target with commercial feasibility for syngas separations.
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http://dx.doi.org/10.1002/anie.202106346DOI Listing
August 2021

Different Infection Profiles and Antimicrobial Resistance Patterns Between Burn ICU and Common Wards.

Front Cell Infect Microbiol 2021 30;11:681731. Epub 2021 Jun 30.

Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Infection is the leading cause of complications and deaths after burns. However, the difference in infection patterns between the burn intensive care unit (BICU) and burn common wards (BCW) have not been clearly investigated. The present study aimed to compare the infection profile, antimicrobial resistance, and their changing patterns in burn patients in BICU and BCW. Clinical samples were analyzed between January 1, 2011, and December 31, 2019, in the Institute of Burn Research in Southwest China. The patient information, pathogen distribution, sources, and antimicrobial resistance were retrospectively collected. A total of 3457 and 4219 strains were detected in BICU and BCW, respectively. Wound secretions accounted for 86.6% and 44.9% in BCW and BICU, respectively. Compared with samples in BCW, samples in BICU had more fungi (11.8% vs. 8.1%), more Gram-negative bacteria (60.0% vs. 50.8%), and less Gram-positive bacteria (28.2% vs. 41.1%). were the most common pathogen in BICU, compared with in BCW. was the most frequent pathogen in wound secretions and tissues from both BICU and BCW. However, were the first in blood, sputum, and catheter samples from BICU. Overall, the multidrug-resistance (MDR) rate was higher in BICU than in BCW. However, the gap between BICU and BCW gradually shortened from 2011 to 2019. The prevalence of MDR and significantly increased, especially in BCW. Furthermore, Carbapenem resistance among significantly increased in BICU (4.5% in 2011 vs. 40% in 2019) and BCW (0 in 2011 vs. 40% in 2019). However, the percentage of MDR sharply dropped from 85.7% to 24.5% in BICU. The incidence of MRSA was significantly higher in BICU than in BCW (94.2% vs. 71.0%) and stayed at a high level in BICU (89.5% to 96.3%). and were the two most frequent fungi. No resistance to Amphotericin B was detected. Our study shows that the infection profile is different between BICU and BCW, and multidrug resistance is more serious in BICU than BCW. Therefore, different infection-control strategies should be emphasized in different burn populations.
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http://dx.doi.org/10.3389/fcimb.2021.681731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278283PMC
July 2021

Ultrasound-guided percutaneous microwave ablation for hepatocellular carcinoma originating in the caudate lobe: A pilot clinical study.

J Cancer Res Ther 2021 Jul;17(3):764-770

Department of Interventional Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Objective: This study aimed to evaluate the efficacy, feasibility, and tolerability of ultrasound (US)-guided percutaneous microwave ablation (MWA) for treating hepatocellular carcinoma (HCC) originating in the caudate lobe.

Materials And Methods: The treatment and survival parameters of 32 patients with HCC in the caudate lobe, who met the inclusion criteria and had received US-guided percutaneous MWA in our department from November 2010 to October 2015, were retrospectively analyzed. Imaging examination (contrast-enhanced computed tomography or magnetic resonance) 1 month after MWA was used to evaluate the efficacy of US-guided MWA.

Results: Thirty-two patients underwent percutaneous MWA for caudate lobe HCC. The average tumor size was 3.42 ± 0.27 (range: 1-6.8) cm. The initial complete ablation (CA) rate was 87.5% (28/32), and the total CA rate was 96.88% (31/32). Furthermore, the median length of hospitalization was 4 days (range: 2-10 days), and no major complication was observed in this study. The overall survival rates were 87.5%, 50%, and 28.13% at 1, 2, and 3 years, respectively. The progression-free survival after MWA was 93.75%, 53.15%, and 28.13% at 6, 12, and 18 months, respectively.

Conclusions: US-guided percutaneous MWA was a safe and effective treatment. It is a promising alternative therapy for HCC originating in the caudate lobe.
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http://dx.doi.org/10.4103/jcrt.JCRT_1851_20DOI Listing
July 2021

An m6A-Related Prognostic Biomarker Associated With the Hepatocellular Carcinoma Immune Microenvironment.

Front Pharmacol 2021 24;12:707930. Epub 2021 Jun 24.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

N6-methyladenosine (m6A) is related to the progression of multiple cancers. However, the underlying influences of m6A-associated genes on the tumor immune microenvironment in hepatocellular carcinoma (HCC) remain poorly understood. Therefore, we sought to construct a survival prediction model using m6A-associated genes to clarify the molecular and immune characteristics of HCC. HCC case data were downloaded from The Cancer Genome Atlas (TCGA). Then, by applying consensus clustering, we identified two distinct HCC clusters. Next, four m6A-related genes were identified to construct a prognostic model, which we validated with Gene Expression Omnibus (GEO) and International Cancer Genome Consortium (ICGC) datasets. Additionally, the molecular and immune characteristics in different subgroups were analyzed. m6A RNA methylation regulators were differentially expressed between HCC and normal samples and linked with immune checkpoint expression. Using consensus clustering, we divided HCC samples into two subtypes with distinct clinical features. Cluster 2 was associated with unfavorable prognosis, higher immune checkpoint expression and immune cell infiltration levels. In addition, the immune and carcinogenic signaling pathways were enriched in cluster 2. Furthermore, we constructed a risk model using four m6A-associated genes. Patients with different risk scores had distinct survival times, expression levels of immunotherapy biomarkers, TP53 mutation rates, and sensitivities to chemotherapy and targeted therapy. Similarly, the model exhibited an identical impact on overall survival in the validation cohorts. The constructed m6A-based signature may be promising as a biomarker for prognostics and to distinguish immune characteristics in HCC.
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http://dx.doi.org/10.3389/fphar.2021.707930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263919PMC
June 2021

[Analysis of genetic variant in a fetus featuring pontocerebellar hypoplasia type 6].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jul;38(7):667-670

Department of Gynecology and Obstetrics, Huaian Maternal and Child Health Care Hospital, Huaian, Jiangsu 223002, China.

Objective: To explore the genetic basis for a fetus with cerebellar dysplasia and widened lateral ventricles.

Methods: The couple have elected induced abortion after careful counseling. Skin tissue sample from the abortus and peripheral venous blood samples from both parents were collected for the extraction of genomic DNA, which was then subjected to whole exome sequencing. Candidate variant was verified by Sanger sequencing.

Results: Prenatal ultrasonography showed increased nuchal translucency (0.4 cm) and widened lateral ventricles. Magnetic resonance imaging revealed infratentorial brain dysplasia. By DNA sequencing, the fetus was found to carry compound heterozygous variants c.1A>G and c.1564G>A of the RARS2 gene, which were inherited from its father and mother, respectively. Among these, c.1A>G was known to be pathogenic, but the pathogenicity of c.1564G>A was unreported previously. Based on the American College of Medical Genetics and Genomics guidelines, the c.1564G>A variant of RARS2 gene was predicted to be likely pathogenic(PM2+PM3+PP3+PP4).

Conclusion: The compound heterozygous variants c.1A>G and c.1564G>A of RARS2 gene contributed to the fetus suffering from pontocerebellar hypoplasia type 6, which expanded variant spectrum of RARS2 gene.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200512-00340DOI Listing
July 2021

Measurement of density profile and fluctuations using a multi-channel terahertz solid-state interferometer system on Keda Torus eXperiment (KTX).

Rev Sci Instrum 2021 May;92(5):053514

University of Science and Technology of China, Hefei, Anhui 230026, China.

A five-chord interferometer based on terahertz solid state sources has been successfully installed on the Keda Torus eXperiment (KTX), a reversed field pinch machine. The optical design has been carefully optimized for the uniform distribution of beam light to fully use the limited power source (∼2 mW). By setting the telescopic mirror unit, the beam waist is located in the center of the vacuum vessel and its diameter is in the range of the Rayleigh length. The beam width across the plasma area is improved to ∼20 mm to minimize crosstalk and beam energy loss. After careful beam alignment, the phase noise for each channel can reach 0.004π. The radial profiles of electron density on the KTX are inverted, and density fluctuation associated with instabilities is shown based on the forward-scattering signals.
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http://dx.doi.org/10.1063/5.0043864DOI Listing
May 2021

3D super-resolution deep-tissue imaging in living mice.

Optica 2021 Apr 25;8(4):442-450. Epub 2021 Mar 25.

Department of Biomedical Engineering, Yale School of Engineering & Applied Science, New Haven, Connecticut 06520, USA.

Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.
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http://dx.doi.org/10.1364/OPTICA.416841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243577PMC
April 2021

Risk Factors and Clinical Outcomes of Cognitive Impairment in Diabetic Patients Undergoing Peritoneal Dialysis.

Kidney Blood Press Res 2021 Jul 6:1-10. Epub 2021 Jul 6.

Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Introduction: Cognitive impairment (CI) is common in patients with CKD or diabetes mellitus (DM). However, the relevance between DM and CI in diabetic patients undergoing peritoneal dialysis (PD) has not been clearly established. This study aimed to explore the role of DM in CI, the association of glycemic control with CI, and clinical outcomes of CI in diabetic PD patients.

Methods: Continuous ambulatory PD (CAPD) patients followed up in our PD center between 2014 and 2016 were enrolled. The participants were followed until an endpoint was reached or December 2017. Demographic data and clinical characteristics were collected, and laboratory parameters were measured. The Montreal Cognitive Assessment (MoCA) was used to evaluate global cognitive function, and a score of <26 was considered to indicate CI. A propensity score matching according to age, gender, and mean arterial pressure was conducted between the DM and non-DM groups.

Results: A total of 913 CAPD patients were enrolled, of whom 186 (20.4%) had diabetes. After appropriate matching, 175 patients in the DM group and 270 patients in the non-DM group were included. Patients with diabetes had a higher prevalence of CI and lower scores for visuospatial/executive function, naming, language, delayed recall, and orientation. Higher HbA1c (odds ratio [OR], 1.547; 95% confidence interval [95% CI], 1.013-2.362) and cardiovascular disease (CVD; OR, 2.926; 95% CI, 1.139-7.516) significantly correlated with a risk of CI in diabetic patients. During a median of 26.0 (interquartile range 13.5-35.6) months of follow-up, diabetic patients with CI demonstrated a significantly lower survival rate than those without CI, and CI was an independent risk factor for mortality after adjustment (hazard ratio, 7.224; 95% CI, 1.694-30.806). However, they did not show worse technique survival or higher peritonitis rate than patients without CI.

Conclusions: HbA1c and CVD are independent risk factors for CI in diabetic patients undergoing CAPD, and CI is independently associated with a higher risk of mortality.
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http://dx.doi.org/10.1159/000514172DOI Listing
July 2021

Sulfur vacancies affect the environmental fate, corona formation, and microalgae toxicity of molybdenum disulfide nanoflakes.

J Hazard Mater 2021 Oct 25;419:126499. Epub 2021 Jun 25.

School of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, China.

Sulfur vacancy (SV) defects have been engineered in two-dimensional (2D) transition metal dichalcogenides (TMDs) for high performance applications in various fields involving environmental protection. Understanding the influence of SVs on the environmental fate and toxicity of TMDs is critical for evaluating their risk. Our work discovered that SVs (with S/Mo ratios of 1.65 and 1.32) reduced the dispersibility and promoted aggregation of 2H phase molybdenum disulfide (2H-MoS, a hot TMD) in aqueous solution. The generation capability of •O and •OH was increased and the dissolution of 2H-MoS was significantly accelerated after SVs formation. Different with pristine form, S-vacant 2H-MoS preferentially harvested proteins (i.e., forming protein corona) involved in antioxidation, photosynthetic electron transport, and the cytoskeleton structure of microalgae. These proteins contain a higher relative number of thiol groups, which exhibited stronger affinity to S-vacant than pristine 2H-MoS, as elucidated by density functional theory calculations. Notably, SVs aggravated algal growth inhibition, oxidative damage, photosynthetic efficiency and cell membrane permeability reduction induced by 2H-MoS due to increased free radical yield and the specific binding of functional proteins. Our findings provide insights into the roles of SVs on the risk of MoS while highlighting the importance of rational design for TMDs application.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126499DOI Listing
October 2021

Exercise Improves Movement by Regulating the Plasticity of Cortical Function in Hemiparkinsonian Rats.

Front Aging Neurosci 2021 14;13:695108. Epub 2021 Jun 14.

Department of Physical Education, China University of Geosciences, Beijing, China.

Aberrant cortical spike-local field potential (LFP) coupling leads to abnormal basal ganglia activity, disruption of cortical function, and impaired movement in Parkinson's disease (PD). Here, the primary motor cortex mediated plasticity mechanism underlying behavioral improvement by exercise intervention was investigated. Exercise alleviates motor dysfunction and induces neuroplasticity in PD. In this study, Sprague-Dawley (SD) rats were injected with 6-hydroxydopamine (6-OHDA) to induce unilateral nigrostriatal dopamine depletion. Two weeks later, a 4-week exercise intervention was initiated in the PD + exercise (Ex) group. Multichannel recording technology recorded spikes and LFPs in rat motor cortices, and balanced ability tests evaluated behavioral performance. The balanced ability test showed that the total crossing time/front leg error/input latency time was significantly lower in PD + Ex rats than in PD rats ( < 0.05). Scalograms and LFP power spectra indicated increased beta-range LFP power in lesioned hemispheres, with exercise reducing LFP power spectral density. Spike-triggered LFP waveform averages showed strong phase-locking in PD motor cortex cells, and exercise reduced spike-LFP synchronization. Our results suggest that exercise can suppress overexcitability of LFPs and minimize spike-LFP synchronization in the motor cortex, leading to motor-improving effects in PD.
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http://dx.doi.org/10.3389/fnagi.2021.695108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236842PMC
June 2021

Use of double-balloon enteroscopy to extract a partial denture embedded in the ileum for 5 weeks.

Br J Hosp Med (Lond) 2021 Jun 30;82(6):1-2. Epub 2021 Jun 30.

Department of Pain Treatment, Tangdu Hospital, Air Force Military Medical University, Xi'an, China.

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http://dx.doi.org/10.12968/hmed.2020.0759DOI Listing
June 2021

The transcription factor RUNX2 fuels YAP1 signaling and gastric cancer tumorigenesis.

Cancer Sci 2021 Sep 16;112(9):3533-3544. Epub 2021 Jul 16.

Department of Cancer Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Despite considerable efforts in the detection and treatment of gastric cancer (GC), the underlying mechanism of the progression of GC remains unknown. Our previous work has demonstrated the remarkable role of Runt-related transcription factor 2 (RUNX2), in fueling the invasion and metastasis of GC. The present study aimed to elucidate the role of RUNX2 in tumorigenesis of GC. We assessed Runx2 expression and its clinical significance via bioinformatic analysis of the Cancer Genome Atlas and Gene Expression Omnibus databases. Roles for Runx2 in self-renewal and tumorigenesis were examined in vitro and in vivo. Further bioinformatic analysis was applied to study the mechanism of GC progression. We found that Runx2 was highly expressed in the early stage of GC and positively correlated with a poor clinical outcome of patients. Runx2 was also significantly correlated with clinicopathological features, such as Hp infection, new neoplastic events, primary therapeutic outcome, ethnicity, race, and tumor stage. Multivariate analysis revealed that together with Runx2, age, cancer status, M stage, and T stage were independent prognostic factors for the outcome of GC patients. RUNX2 overexpression induced increased anchorage-independent colony formation, sphere formation, and tumorigenesis in GC cells in vitro and in vivo. Mechanistically, bioinformatic analysis indicated that yes1 associated transcriptional regulator (YAP1) might be a downstream target of RUNX2. Specific knockdown of YAP1 reduced the tumor-initiating ability of GC cells induced by ectopic Runx2 expression. Our findings support the hypothesis that RUNX2 exerts oncogenic properties via YAP1 regulation, highlighting essential roles for RUNX2 and YAP1 in gastric carcinogenesis and suggesting potential therapeutic targets.
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http://dx.doi.org/10.1111/cas.15045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409423PMC
September 2021

Safety and Efficacy of a Phage, kpssk3, in an Model of Carbapenem-Resistant Hypermucoviscous Bacteremia.

Front Microbiol 2021 20;12:613356. Epub 2021 May 20.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Antimicrobial resistance (AMR) is one of the most significant threats to global public health. As antibiotic failure is increasing, phages are gradually becoming important agents in the post-antibiotic era. In this study, the therapeutic effects and safety of kpssk3, a previously isolated phage infecting carbapenem-resistant hypermucoviscous (CR-HMKP), were evaluated in a mouse model of systemic CR-HMKP infection. The therapeutic efficacy experiment showed that intraperitoneal injection with a single dose of phage kpssk3 (1 × 10 PFU/mouse) 3 h post infection protected 100% of BALB/c mice against bacteremia induced by intraperitoneal challenge with a 2 × LD dose of NY03, a CR-HMKP clinical isolate. In addition, mice were treated with antibiotics from three classes (polymyxin B, tigecycline, and ceftazidime/avibactam plus aztreonam), and the 7 days survival rates of the treated mice were 20, 20, and 90%, respectively. The safety test consisted of 2 parts: determining the cytotoxicity of kpssk3 and evaluating the short- and long-term impacts of phage therapy on the mouse gut microbiota. Phage kpssk3 was shown to not be cytotoxic to mammalian cells or . Fecal samples were collected from the phage-treated mice at 3 time points before (0 day) and after (3 and 10 days) phage therapy to study the change in the gut microbiome via high-throughput 16S rDNA sequence analysis, which revealed no notable alterations in the gut microbiota except for decreases in the Chao1 and ACE indexes.
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http://dx.doi.org/10.3389/fmicb.2021.613356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175031PMC
May 2021

Early initiation of PD therapy in elderly patients is associated with increased risk of death.

Clin Kidney J 2021 Jun 12;14(6):1649-1656. Epub 2020 Dec 12.

Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: The effect of early initiation of dialysis on outcomes of patients with end-stage renal disease (ESRD) remains controversial. We conducted this study to investigate the association between the timing of peritoneal dialysis (PD) initiation and mortality in different age groups.

Methods: In this single-centre cohort study, incident patients receiving PD from 1 January 2006 to 31 December 2016 were enrolled. Patients were categorized into three groups according to the estimated glomerular filtration rate (eGFR) at the initiation of PD, with early, mid and late initiation of PD defined as eGFR ≥7.5, 5-7.5 and <5 mL/min/1.73 m, respectively.

Results: A total of 2133 incident patients receiving PD were enrolled with a mean age of 47.1 years, 59.6% male and 25.3% with diabetes, of whom 1803 were young (age <65 years) and 330 were elderly (age ≥65 years). After multivariable adjustment, the overall and cardiovascular (CV) mortality risks for young patients receiving PD were not significantly different between these three groups. However, for elderly patients, early initiation of PD therapy was associated with increased risks of all-cause {hazard ratio [HR} 1.54 [95% confidence interval (CI) 1.06-2.25]} and CV [HR 2.07 (95% CI 1.24-3.48)] mortality compared with late initiation of PD, while no significant difference was observed in overall or CV mortality between the mid- and late-start groups.

Conclusions: No significant difference in mortality risk was found among the three levels of eGFR at PD therapy initiation in young patients, while early initiation of PD was associated with a higher risk of overall and CV mortality among elderly patients.
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http://dx.doi.org/10.1093/ckj/sfaa214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162869PMC
June 2021

A highly sensitive method for simultaneous detection of hAAG and UDG activity based on multifunctional dsDNA probes mediated exponential rolling circle amplification.

Talanta 2021 Sep 24;232:122429. Epub 2021 Apr 24.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China. Electronic address:

DNA glycosylase is an indispensable DNA damage repair enzyme which can recognize and excise the damaged bases in the DNA base excision-repair pathway. The dysregulation of DNA glycosylase activity will give rise to the dysfunction of base excision-repair and lead to abnormalities and diseases. The simultaneous detection of multiple DNA glycosylases can help to fully understand the normal physiological functions of cells, and determine whether the cells are abnormal in pre-disease. Regrettably, the synchronous detection of functionally similar DNA glycosylases is a great challenge. Herein, we developed a multifunctional dsDNA probe mediated exponential rolling circle amplification (E-RCA) method for the simultaneously sensitive detection of human alkyladenine DNA glycosylase (hAAG) and uracil-DNA glycosylase (UDG). The multifunctional dsDNA probe contains the hypoxanthine sites and the uracil sites which can be recognized by hAAG and UDG respectively to generate apyrimidinic (AP) sites in the dsDNA probe. Then the AP sites will be recognized and cut by endonuclease Ⅳ (Endo IV) to release corresponding single-stranded primer probes. Subsequently, two padlock DNA templates are added to initiate E-RCA to generate multitudinous G-quadruplexes and/or double-stranded dumbbell lock structures, which can combine N-methyl mesoporphyrin IX (NMM) and SYBR Green Ⅰ (SGI) for the generation of respective fluorescent signals. The detection limits are obtained as low as 0.0002 U mL and 0.00001 U mL for hAAG and UDG, respectively. Notably, this method can realize the simultaneous detection of two DNA glycosylases without the use of specially labeled probes. Finally, this method is successfully applied to detect hAAG and UDG activities in the lysates of HeLa cells and Endo1617 cells at single-cell level, and to detect the inhibitors of DNA glycosylases.
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http://dx.doi.org/10.1016/j.talanta.2021.122429DOI Listing
September 2021
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