Publications by authors named "Peng Xu"

2,119 Publications

  • Page 1 of 1

N-Ethylnorketamine has anesthetic and analgesic effects with abuse liability.

Behav Brain Res 2022 Aug 8:114052. Epub 2022 Aug 8.

School of Pharmacy, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, PR China; Office of China National Narcotics Control Commission, China Pharmaceutical University Joint Laboratory on Key Technologies of Narcotics Control, Beijing 100193, PR China. Electronic address:

Arylcyclohexylamines is an ever-growing class of new psychoactive substances, including an increasing number of ketamine analogues. N-Ethylnorketamine (NENK) is a new synthetic ketamine analogue that has emerged as an abused drug, but little is known about the pharmacological profile of NENK. In this study, we investigated the anesthetic and analgesic activity, abuse liability of NENK compared with ketamine. The ED values of anesthetic activity for NENK and ketamine were 96.9, 69.4mg/kg, respectively. The ED values of analgesic activity for NENK and ketamine were 45.9 and 23.6mg/kg, respectively. NENK induced significant conditioned place preference at a minimum dose of 10.0mg/kg in mice, an effect comparable to that of ketamine (3.0mg/kg). Acute injections of NENK or ketamine at 30.0mg/kg enhanced locomotor activity, and repeated treatments with this dose induced locomotor sensitization after withdrawal. Taken together, these results clearly demonstrated that NENK has lower anesthetic and analgesic activity compared to ketamine, but has significant abuse liability.
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http://dx.doi.org/10.1016/j.bbr.2022.114052DOI Listing
August 2022

LncRNA-p21 suppresses cell proliferation and induces apoptosis in gastric cancer by sponging miR-514b-3p and up-regulating ARHGEF9 expression.

Biol Chem 2022 Aug 11. Epub 2022 Aug 11.

Department of Oncology, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an 710004, Shaanxi, China.

The long non-coding RNA p21 (lncRNA-p21) was a tumor suppressor gene in most cancer types including gastric cancer (GC). We aimed to identify a specific lncRNA-p21-involved pathway in regulating the proliferation and apoptosis of GC cells. A lower lncRNA-p21 expression in tumors was associated with advanced disease stage and predicted worse survival of GC patients. LncRNA-p21 overexpression in GC cell line somatic gastric cancer (SGC)-7901 and human gastric cancer (HGC)-27 suppressed cell proliferation and enhanced apoptosis, while lncRNA-p21 knockdown caused the opposite effects. Through bioinformatics analysis and luciferase-based reporter assays, we identified miR-514b-3p as a sponge target of lncRNA-p21. Cdc42 guanine nucleotide exchange factor 9 (ARHGEF9), functioned as a tumor suppress factor in GC, was found as the downstream target of miR-514-3p, and their expressions were negatively correlated in GC tumor tissues. In addition, like lncRNA-p21 overexpression alone, miR-514-3p inactivation alone also led to decreased proliferation and increased apoptosis in SGC-7901 and HGC-27 cells, which were markedly attenuated by additional ARHGEF9 knockdown. Xenograft SGC-7901 cells with more lncRNA-p21 or ARHGEF9 expressions or with less miR-514-3p expression exhibited obviously slower growth than the control SGC-7901 cells in nude mice. Our study reveals a novel lncRNA-p21/miR-514b-3p/ARHGEF9 pathway that can be targeted for GC therapy.
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http://dx.doi.org/10.1515/hsz-2022-0153DOI Listing
August 2022

Well-designed two-fold crosslinked biological valve leaflets with heparin-loaded hydrogel coating for enhancing anticoagulation, endothelialization, and anticalcification.

Biomater Sci 2022 Aug 10. Epub 2022 Aug 10.

College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, P.R. China.

Commercial biological valve leaflets (BVLs) crosslinked with glutaraldehyde (GA) are at risk of accelerating damage and even failure, owing to the high cell toxicity of GA, acute thrombosis, and calcification in clinical applications. In this study, a novel joint strategy of double crosslinking agents (dialdehyde pectin (AP) and carbodiimide) and heparin-loaded hydrogel coating was developed, endowing BVLs with excellent mechanical properties and multiple performances. Herein, AP played two essential roles, the crosslinking agent and the main component of the hydrogel coating. Both experimental and theoretical results indicated that the mechanical properties and stability of double-crosslinked BVLs were comparable to those of GA, and heparin loaded in hydrogel coating could improve the hemocompatibility of AP + EDC/NHS-PP. Further, cytocompatibility and tests showed that compared with GA-PP, AP + EDC/NHS + CS + Hep-PP has exhibited good endothelialization ability, mild immune response and anti-calcification performance and therefore prompts it to be an extremely valuable candidate for more durable and multifunctional BVLs.
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http://dx.doi.org/10.1039/d2bm00736cDOI Listing
August 2022

MicroRNA-147a Targets SLC40A1 to Induce Ferroptosis in Human Glioblastoma.

Anal Cell Pathol (Amst) 2022 30;2022:2843990. Epub 2022 Jul 30.

Hangzhou Chinese Academy of Sciences-Hangzhou Medical College Advanced Medical Technology Institute, Hangzhou, 310018 Zhejiang, China.

Objective: Glioblastoma is one of the most common malignant tumors in the brain, and these glioblastoma patients have very poor prognosis. Ferroptosis is involved in the progression of various tumors, including the glioblastoma. This study aims to determine the involvement of microRNA (miR)-147a in regulating ferroptosis of glioblastoma in vitro.

Methods: Human glioblastoma cell lines were transfected with the inhibitor, mimic and matched negative controls of miR-147a in the presence or absence of ferroptotic inducers. To knock down the endogenous solute carrier family 40 member 1 (SLC40A1), cells were transfected with the small interfering RNA against SLC40A1. In addition, cells with or without the miR-147a mimic treatment were also incubated with temozolomide (TMZ) to investigate whether miR-147a overexpression could sensitize human glioblastoma cells to TMZ chemotherapy in vitro.

Results: We found that miR-147a level was decreased in human glioblastoma tissues and cell lines and that the miR-147a mimic significantly suppressed the growth of glioblastoma cells in vitro. In addition, miR-147a expression was elevated in human glioblastoma cells upon erastin or RSL3 stimulation. Treatment with the miR-147a mimic significantly induced ferroptosis of glioblastoma cells, and the ferroptotic inhibitors could block the miR-147a mimic-mediated tumor suppression in vitro. Conversely, the miR-147a inhibitor prevented erastin- or RSL3-induced ferroptosis and increased the viability of glioblastoma cells in vitro. Mechanistically, we determined that miR-147a directly bound to the 3'-untranslated region of SLC40A1 and inhibited SLC40A1-mediated iron export, thereby facilitating iron overload, lipid peroxidation, and ferroptosis. Furthermore, miR-147a mimic-treated human glioblastoma cells exhibited higher sensitivity to TMZ chemotherapy than those treated with the mimic control in vitro.

Conclusion: We for the first time determine that miR-147a targets SLC40A1 to induce ferroptosis in human glioblastoma in vitro.
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http://dx.doi.org/10.1155/2022/2843990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356897PMC
August 2022

The clock component OsLUX regulates rice heading through recruiting OsELF3-1 and OsELF4s to repress Hd1 and Ghd7.

J Adv Res 2022 Aug 6. Epub 2022 Aug 6.

China National Center for Rice Improvement and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. Electronic address:

Introduction: Circadian clocks coordinate internal physiology and external environmental factors to regulate cereals flowering, which is critical for reproductive growth and optimal yield determination.

Objectives: In this study, we aimed to confirm the role of OsLUX in flowering time regulation in rice. Further research illustrates how the OsELF4s-OsELF3-1-OsLUX complex directly regulates flowering-related genes to mediate rice heading.

Methods: We identified a circadian gene OsLUX by the MutMap method. The transcription levels of flowering-related genes were evaluated in WT and oslux mutants. OsLUX forms OsEC (OsELF4s-OsELF3-1-OsLUX) complex were supported by yeast two-hybrid, pull down, BiFC, and luciferase complementation assays (LCA). The EMSA, Chip-qPCR, luciferase luminescence images, and relative LUC activity assays were performed to examine the targeted regulation of flowering genes by the OsEC (OsELF4s-OsELF3-1-OsLUX) complex.

Results: The circadian gene OsLUX encodes an MYB family transcription factor that functions as a vital circadian clock regulator and controls rice heading. Defect in OsLUX causes an extremely late heading phenotype under natural long-day and short-day conditions, and the function was further confirmed through genetic complementation, overexpression, and CRISPR/Cas9 knockout. OsLUX forms the OsEC (OsELF4s-OsELF3-1-OsLUX) complex by recruiting OsELF3-1 and OsELF4s, which were required to regulate rice heading. OsELF3-1 contributes to the translocation of OsLUX to the nucleus, and a compromised flowering phenotype results upon mutation of any component of the OsEC complex. The OsEC complex directly represses Hd1 and Ghd7 expression via binding to their promoter's LBS (LUX binding site) element.

Conclusion: Our findings show that the circadian gene OsLUX regulates rice heading by directly regulating rhythm oscillation and core flowering-time-related genes. We uncovered a mechanism by which the OsEC target suppresses the expression of Hd1 and Ghd7 directly to modulate photoperiodic flowering in rice. The OsEC (OsELF4s-OsELF3-1-OsLUX)-Hd1/Ghd7 regulatory module provides the genetic targets for crop improvement.
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http://dx.doi.org/10.1016/j.jare.2022.08.001DOI Listing
August 2022

A simple pre-disease state prediction method based on variations of gene vector features.

Comput Biol Med 2022 Jul 19;148:105890. Epub 2022 Jul 19.

Department of Computer Science, Rogers School of Engineering and Computer Science, Baylor University, TX, 76798, Waco, USA. Electronic address:

Background: The progression of disease can be divided into three states: normal, pre-disease, and disease. Since a pre-disease state is the tipping point of disease deterioration, accurately predicting pre-disease state may help to prevent the progression of disease and develop feasible treatment in time.

Methods: In the perspective of gene regulatory network, the expression of a gene is regulated by its upstream genes, and then it also regulates that of its downstream genes. In this study, we define the expression value of these genes as a gene vector to depict its state in a specific sample. Then, we propose a novel pre-disease prediction method by such vector features.

Results: The results of an influenza virus infection dataset show that our method can successfully predict the pre-disease state. Furthermore, the pre-disease state related genes predicted by our methods are highly associated with each other and enriched in influenza virus infection related pathways. In addition, our method is more time efficient in calculation than previous works. The code of our method is accessed at https://github.com/ZhenshenBao/sPGVF.git.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105890DOI Listing
July 2022

Long-term outcomes of bipolar transurethral enucleation and resection of the prostate on patients with benign prostatic obstruction: a 10-year follow-up.

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2022 Jul 18. Epub 2022 Jul 18.

Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China.

Objective: We aimed to explore the long-term outcomes of bipolar transurethral enucleation and resection of the prostate (B-TUERP) in patients with benign prostatic hyperplasia (BPH).

Methods: A total of 1195 patients with BPH who underwent B-TUERP from July 2006 to June 2010 were enrolled in this retrospective study. Preoperative data, particularly urodynamic study (UDS) parameters, were collected by questionnaire and examination. Postoperative follow-up was performed at 3, 6, 12, 36, 60 and 120 months, respectively. Demographic, perioperative and functional data were analysed. International Prostate Symptom Score, quality of life and overactive bladder syndrome score (OABSS) were used to assess the functional scores. Predictors of postoperative urgency incontinence were identified by univariate analysis.

Results: Long-term outcomes of functional score were significantly better than the preoperative values. All UDS variables, including maximum urinary flow rate, postvoid residual urine volume, compliance, maximum cystometric capacity (MCC), bladder outlet obstruction index, prostatic urethral pressure and detrusor pressure at maximum urinary flow rate, were all significantly improved. No one required reoperation due to recurrent BPH. The postoperative rate of transient urinary incontinence was 31.7%, while the long-term incontinence rate was 0%. Retrograde ejaculation occurred in 44.3% patients who remained sexually active after operation. Patients who had transient urge incontinence were older with preoperative higher OABSS, prostate-specific antigen (PSA) and detrusor overactivity rate and lower MCC.

Conclusion: B-TUERP is a safe, successful and highly effective treatment for BPH at 10-year follow-up. Increased age, OABSS, PSA level and detrusor overactivity rate are potential predictors for urge incontinence after B-TUERP.
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http://dx.doi.org/10.5507/bp.2022.034DOI Listing
July 2022

Functionalized zinc oxide microparticles for improving the antimicrobial effects of skin-care products and wound-care medicines.

Biomater Adv 2022 Apr 22;135:212728. Epub 2022 Apr 22.

College of Chemistry, Fuzhou University, Fujian 350116, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350116, China. Electronic address:

ZnO is an important component in skin-protection products and wound-care medicines. However, ZnO's antibacterial activity is moderate. We developed two types of ZnO microparticles loading with phthalocyanine-type photosensitizers (ZnO/PSs) introducing the photodynamic effects. These photosensitive ZnO microparticles exhibited long-term while moderate antimicrobial effects by continuously releasing Zn ions. The antimicrobial efficacies were remarkably enhanced by triggering the photodynamic antimicrobial effects. Compared to the sole ZnO which showed non-measurable antimicrobial activity at a concentration of 10 mg/L, both ZnO/PSs demonstrated antimicrobial rates ranged 99%-99.99% against Escherichia coli, normal and drug-resistant Staphylococcus aureus. In a dorsal wound infection mouse model, treatment with ZnO/PSs significantly accelerated the wound recovery rates. ZnO/PSs promoted wound healing by a dual effect: 1) the release of Zn ions from ZnO facilitating tissue remodeling; 2) the photodynamic effect efficiently eliminates pathogens avoiding infection. Notably, ZnO/PSs inherited the high biosafety of ZnO without causing noticeable toxicity against erythrocyte and endothelial cells. This study not only provides a highly safe and efficient antimicrobial ZnO material for skin cares and wound modulations, but also proposes a strategy to functionalize ZnO materials.
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http://dx.doi.org/10.1016/j.bioadv.2022.212728DOI Listing
April 2022

The impact of heterogeneous environmental regulations on regional spatial differences in net carbon emissions.

Authors:
Peng Xu

Environ Sci Pollut Res Int 2022 Aug 2. Epub 2022 Aug 2.

Department of Environmental Science and Engineering, Fudan University, Shanghai, 200438, People's Republic of China.

Effected by spatial difference and types of environmental regulation, enhanced environmental regulation does not necessarily lead to reduce net carbon emissions (NCE). Based on panel data of 30 province-level administrative regions in China from 2003 to 2018, this paper analyzed the impact of heterogeneous environmental regulations on NCE using geographically weighted regression (GWR). The results showed that (1) the total NCE of each province in China presented an increasing trend followed by fluctuation, with obvious spatial aggregation characteristics. (2) The intensity of four environmental regulations, command-and-control (CAC), market-incentive (MI), public-participation (PP), and voluntary environmental program of firm (VEPF) tended to increase slightly over the study period with decreasing spatial heterogeneity. (3) CAC environmental regulations had a "green paradox" effect on NCE, meaning an improving CAC environmental regulation would surprisingly increase its negative effects and contribute to NCE growth. The effect of MI environmental regulation on NCE shifted from overall promotion to partly promotion and even suppression. PP and VEPF environmental regulation would reduce NCE. The suppressive effect of PP environmental regulation grew over time, while VEPF did not. Finally, based on the research conclusion, some policy suggestion are proposed for differentiated management, diverse system of governance, and collaborative governance.
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http://dx.doi.org/10.1007/s11356-022-22282-8DOI Listing
August 2022

Induction of CYP450 by illicit drugs: Studies using an in vitro 3D spheroidal model in comparison to animals.

Toxicol Lett 2022 Jul 29;367:88-95. Epub 2022 Jul 29.

China National Narcotics Control Commission - China Pharmaceutical University Joint Laboratory on Key Technologies of Narcotics Control, No. 24 Tongjiaxiang Road, Nanjing 210009, China. Electronic address:

Information regarding the metabolism of illicit drugs is under urgent need for toxicological assessment. Its development, however, is limited by the currently available animal models. To this end, we proposed three-dimensional (3D) HepaRG spheroids as an in vitro model to study the effects of illicit drugs on hepatic cytochrome P450 (CYP450) enzymes and potential drug-drug interactions (DDIs). By comparing the results from animal and cell experiments, we confirmed the significant impact of heroin, morphine, tetrahydrocannabinol, and fentanyl on CYP450 enzymes, and the 3D spheroids results were in good agreement with the animal results for 2B6, 2C19, 2D6. Using 3D HepaRG spheroids, we demonstrated DDIs between heroin as a 2B6 perpetrator and clinical medicine for cancer, depression, and illicit drug withdrawal. Specifically, the clearance rate of 5.4 μM bupropion was increased by 214 % under DDI with 5 µM heroin, highlighting the importance of DDI pre-screening and individualized medication guidance for illicit drug users. This research contributes to the growing body of evidence regarding the metabolic toxicity of illicit drugs and suggests 3D HepaRG spheroids as a high-throughput and cost-efficient platform for DDI analysis.
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http://dx.doi.org/10.1016/j.toxlet.2022.07.815DOI Listing
July 2022

The Prognostic Value of Tumor Size, Volume and Tumor Volume Reduction Rate During Concurrent Chemoradiotherapy in Patients With Cervical Cancer.

Front Oncol 2022 14;12:934110. Epub 2022 Jul 14.

Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Prevention and Control Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Objective: This study aimed to investigate the relationship between prognostic and tumor parameters of cervical cancer patients, such as tumor size (TS), tumor volume (TV), and tumor volume reduction rate (TVRR) after external beam radiotherapy.

Methods: A total of 217 patients with advanced cervical cancer, classified as Federation of Gynecology and Obstetrics (FIGO) IIa-IVa, were enrolled in the study. Pre- and mid-RT pelvic magnetic resonance imaging (MRI) were performed twice, during RT and just before brachytherapy.

Results: The median follow-up time was 51 months (range, 7-111 months). The 5-year overall survival (OS), progression-free survival (PFS), and local failure-free survival (LFFS) rates were 81.3, 85.1, and 92.9%, respectively. Multivariate analysis revealed that tumor parameters including FIGO stage >II (Hazard Ratio, 2.377 and 95% confidence interval [CI], 1.091-5.182; = 0.029), pre-RT TV >61.6 cm (HR, 0.417 and 95% CI, 0.188-0.926; = 0.032), and mid-RT TV >11.38 cm (HR, 3.192 and 95% CI, 1.094-9.316; = 0.034) were observably associated with OS. Univariate analysis showed that the tumor volume reduction rate (TVRR) was dramatically associated with overall survival (HR, 0.204 and 95% CI 0.033-1.282; 0.001) and local failure-free survival ( = 0.050).

Conclusions: In this retrospective study, TVRR and mid-radiotherapy tumor volume are independent and strong prognostic parameters for patients with local advanced cervical cancer receiving CCRT.
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http://dx.doi.org/10.3389/fonc.2022.934110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329537PMC
July 2022

The genome-wide identification and adaptive evolution of slc9 genes in Leuciscus waleckii under extremely alkaline conditions.

Gene 2022 Oct 27;840:146769. Epub 2022 Jul 27.

State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian, China; Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China. Electronic address:

The solute carrier family 9 (slc9) genes, especially slc9a isoform coding proteins contribute to electroneutral countertransport of H for Na across the plasmalemmal and organellar membranes, intracellular pH and cellular volume regulation as well as the electrolyte, acid-base, and fluid volume homeostasis at the systemic level. These functional properties determine a potential basis for organisms to challenge stressful conditions. However, these well-done researches have been reported more in mammals. Thus, in this study, a total of eleven slc9 genes were identified from the latest version genome of L. waleckii, a cyprinid fish that could tolerate extremely alkaline environments (pH 9.6). The evolutionary footprint of slc9 genes was uncovered via the analysis of copy numbers, gene structure, motif composition, chromosome location and phylogenetic relationship. More importantly, there were two SNPs located on 5' UTR and three non-synonymous mutations in the coding region of the slc9a3.2 gene by comparing freshwater with alkaline water populations attached to resequencing technology. Slc9a3.2 gene was a statistically significant low expression in gill tissue with extremely alkaline pressure. Generally, slc9 gene family in L. waleckii was highly conserved. Several important SNPs with high Fst values were identified where non-synonymous mutations occurred between freshwater and alkaline water populations, and they may play an important role in specific functional differentiation. Slc9 genes had clear tissue expression preferences and were involved in abiotic stress response, indicating their roles in physiological function and strong self-regulating capacity. Our insight into the genetic variations that take place in the individual genes under extreme conditions could provide a feasible example for studying specific molecular mechanisms based on genomic data with increasing environmental stress.
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http://dx.doi.org/10.1016/j.gene.2022.146769DOI Listing
October 2022

Lymph node metastasis as the initial symptom of a germ cell tumor in an adult: A case report.

Medicine (Baltimore) 2022 Jul 29;101(30):e29281. Epub 2022 Jul 29.

Department of Radiation Oncology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Rationale: Germ cell tumors in the head and neck are very rare. In cases of germ cell tumors, it is uncommon for lymph node metastasis to be the only and initial symptom, and this can easily lead to a misdiagnosis. Herein, we report about a 28-year-old woman with lymph node metastasis, in whom a primary tumor appeared in the nasal cavity.

Patient Concerns: A 28-year-old woman presented with enlarged left submandibular lymph nodes. No other mass was found on whole-body screening using positron emission tomography-computed tomography.

Diagnosis: After partial submandibular lymphadenectomy was performed, histopathological and immunohistochemical examinations revealed a metastatic germ cell tumor. However, it was difficult to further classify and affirm the origin.

Interventions: As the patient was receiving four cycles of bleomycin, etoposide, and cisplatin chemotherapy, a primary tumor emerged in the nasal cavity, which was finally confirmed as an immature teratoma of a high World Health Organization histological grade and Norris grade 3. This tumor was found to contain similar components to lymph nodes with respect to histopathological and immunohistochemical characteristics, especially the immature neural tubes or nervous tissue in the nasal cavity. Fortunately, the patient recovered well with no signs of relapse, and the size of residual lymph nodes remained unchanged after she received another four cycles of bleomycin, etoposide, and cisplatin chemotherapy and two cycles of doxorubicin and ifosfamide (AI) chemotherapy.

Outcomes: Unfortunately, 11 months later, during the coronavirus disease pandemic, the patient died owing to respiratory failure and pulmonary infection.

Conclusions: In cases of malignant tumor in the submandibular lymph nodes of adults, the metastasis of a germ cell tumor should be considered an important differential diagnosis even if a primary tumor does not emerge. In this case, adequate postoperative chemotherapy is necessary.
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http://dx.doi.org/10.1097/MD.0000000000029281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333472PMC
July 2022

Ubiquitination drives COPI priming and Golgi SNARE localization.

Elife 2022 Jul 29;11. Epub 2022 Jul 29.

Department of Biological Sciences, Vanderbilt University, Nashville, United States.

Deciphering mechanisms controlling SNARE localization within the Golgi complex is crucial to understanding protein trafficking patterns within the secretory pathway. SNAREs are also thought to prime COPI assembly to ensure incorporation of these essential cargoes into vesicles, but the regulation of these events is poorly understood. Here we report roles for ubiquitin recognition by COPI in SNARE trafficking and in stabilizing interactions between Arf, COPI, and Golgi SNAREs in . The ability of COPI to bind ubiquitin, but not the dilysine motif, through its N-terminal WD repeat domain of β'COP or through an unrelated ubiquitin-binding domain (UBD) is essential for the proper localization of Golgi SNAREs Bet1 and Gos1. We find that COPI, the ArfGAP Glo3 and multiple Golgi SNAREs are ubiquitinated. Notably, the binding of Arf and COPI to Gos1 is markedly enhanced by ubiquitination of these components. Glo3 is proposed to prime COPI-SNARE interactions; however, Glo3 is not enriched in the ubiquitin-stabilized SNARE-Arf-COPI complex but is instead enriched with COPI complexes that lack SNAREs. These results support a new model for how posttranslational modifications drive COPI priming events crucial for Golgi SNARE localization.
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http://dx.doi.org/10.7554/eLife.80911DOI Listing
July 2022

Enhanced ion transport in nanochannels of MXenes by Mg pre-intercalation.

Phys Chem Chem Phys 2022 Aug 10;24(31):18824-18829. Epub 2022 Aug 10.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, P. R. China.

How to enhance the ion transport between MXene layers is a critical topic in the fields of electrochemical storage (especially supercapacitors) and water treatment. Vertical structure design of MXene nanosheets and single-molecule organic pre-intercalation are proposed, but the methods to enhance the ion transport through MXene nanochannels by modulating MXene's surface state have not been investigated yet. The interaction mechanism between Mg and MXene 2D nanochannels during the transport process has not been thoroughly explored. In our work, we used a facile infiltration method to immerse the TiCT membranes in MgCl solution for ion pre-intercalation. We found that the pre-intercalation of Mg has a significant effect on the increase of the ion transport rate of TiCT membranes, especially for Li which reached 268.49% compared with those of non-intercalation membranes. Through multiple characterization methods, we discovered that the enhancement of ion transport rate by pre-intercalation of Mg mainly originated from the fact that the pre-intercalation of Mg increased the layer spacing of MXene films as the channel support between layers while Mg increased the work function (WF) of 2D nanochannels thereby reducing the interaction of other ions with the channel surface. The acceleration phenomenon of ion transport by surface state modulation proposed in our work will provide new strategies for the design of structure and regulation of surface states, revealing the mechanism of capacity improvement.
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http://dx.doi.org/10.1039/d2cp01700hDOI Listing
August 2022

Predicting individual muscle fatigue tolerance by resting-state EEG brain network.

J Neural Eng 2022 Aug 11;19(4). Epub 2022 Aug 11.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, People's Republic of China.

Exercise-induced muscle fatigue is a complex physiological phenomenon involving the central and peripheral nervous systems, and fatigue tolerance varies across individuals. Various studies have emphasized the close relationships between muscle fatigue and the brain. However, the relationships between the resting-state electroencephalogram (rsEEG) brain network and individual muscle fatigue tolerance remain unexplored.Eighteen elite water polo athletes took part in our experiment. Five-minute before- and after-fatigue-exercise rsEEG and fatiguing task (i.e. elbow flexion and extension) electromyography (EMG) data were recorded. Based on the graph theory, we constructed the before- and after-task rsEEG coherence network and compared the network differences between them. Then, the correlation between the before-fatigue rsEEG network properties and the EMG fatigue indexes when a subject cannot keep on exercising anymore was profiled. Finally, a prediction model based on the before-fatigue rsEEG network properties was established to predict fatigue tolerance.. Results of this study revealed the significant differences between the before- and after-exercise rsEEG brain network and found significant high correlations between before-exercise rsEEG network properties in the beta band and individual muscle fatigue tolerance. Finally, an efficient support vector regression (SVR) model based on the before-exercise rsEEG network properties in the beta band was constructed and achieved the accurate prediction of individual fatigue tolerance. Similar results were also revealed on another 30 subject swimmer data set further demonstrating the reliability of predicting fatigue tolerance based on the rsEEG network.Our study investigates the relationship between the rsEEG brain network and individual muscle fatigue tolerance and provides a potential objective physiological biomarker for tolerance prediction and the regulation of muscle fatigue.
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http://dx.doi.org/10.1088/1741-2552/ac8502DOI Listing
August 2022

Smart Internal Bio-Glues.

Adv Sci (Weinh) 2022 Jul 28:e2203587. Epub 2022 Jul 28.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China.

Although smart bio-glues have been well documented, the development of internal bio-glues for non-invasive or minimally invasive surgery is still met with profound challenges such as safety risk and the lack of deep tissue penetration stimuli for internal usage. Herein, a series of smart internal bio-glues are developed via the integration of o-nitrobenzene modified biopolymers with up-conversion nanoparticles (UCNPs). Upon irradiation by near-infrared (NIR) light, the prepared smart bio-glues can undergo a gelation process, which may further induce strong adhesion between tissues under both dry and wet conditions based on multi-interactions. Moreover, those NIR light-responsive bio-glues with deeper tissue penetration ability demonstrate good biocompatibility, excellent hemostatic performance, and the potent ability to accelerate wound healing for both external and internal wounds. This work provides new opportunities for minimally invasive surgery, especially in internal wound healing using smart and robust bio-glues.
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http://dx.doi.org/10.1002/advs.202203587DOI Listing
July 2022

Disruption of mstn Gene by CRISPR/Cas9 in Large Yellow Croaker (Larimichthys crocea).

Mar Biotechnol (NY) 2022 Jul 27. Epub 2022 Jul 27.

Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.

The large yellow croaker (Larimichthys crocea) plays an economically vital role in the marine aquaculture in China. Suffering from infection of bacteria and protozoon, effect of extreme weather and stress from high-density farming, genome editing is thought to be an important tool applied to L. croea for enhancing commercial traits such as growth rate, disease resistance, and nutrition component. In this study, we identified two mstn genes in L. croea and investigated the different phylogenetic clades, gene structures, and conserved syntenic relationships. To obtain fast-growing large yellow croaker, we specially selected two validated targets for mstnb knockout, which was homologous to mammalian myostatin gene (MSTN) and downregulated skeletal muscle growth and development. Five significant mutation types were generated in two mosaic mutants by transferring specific CRISPR/Cas9 RNPs (ribonucleoprotein) into the one-cell fertilized embryos based on CRISPR/Cas9 technology. Subsequently, we also elucidated the obstacles and possible measures to improve the success rate of inducing modified large yellow croaker. Our results would provide valuable method and reference for facilitating genome editing programs of the large yellow croaker in the future.
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http://dx.doi.org/10.1007/s10126-022-10135-xDOI Listing
July 2022

Miconazole exerts disease-modifying effects during epilepsy by suppressing neuroinflammation via NF-κB pathway and iNOS production.

Neurobiol Dis 2022 Jul 22;172:105823. Epub 2022 Jul 22.

Department of Neurology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou 310052, China. Electronic address:

Neuroinflammation contributes to the generation of epilepsy and has been proposed as an effective therapeutic target. Recent studies have uncovered the potential effects of the anti-fungal drug miconazole for treating various brain diseases by suppressing neuroinflammation but have not yet been studied in epilepsy. Here, we investigated the effects of different doses of miconazole (5, 20, 80 mg/kg) on seizure threshold, inflammatory cytokines release, and glial cells activation in the pilocarpine (PILO) pentylenetetrazole (PTZ), and intrahippocampal kainic acid (IHKA) models. We demonstrated that 5 and 20 mg/kg miconazole increased seizure threshold, but only 20 mg/kg miconazole reduced inflammatory cytokines release, glial cells activation, and morphological alteration during the early post-induction period (24 h, 3 days). We further investigated the effects of 20 mg/kg miconazole on epilepsy (4 weeks after KA injection). We found that miconazole significantly attenuated cytokines production, glial cells activation, microglial morphological changes, frequency and duration of recurrent hippocampal paroxysmal discharges (HPDs), and neuronal and synaptic damage in the hippocampus during epilepsy. In addition, miconazole suppressed the KA-induced activation of the NF-κB pathway and iNOS production. Our results indicated miconazole to be an effective drug for disease-modifying effects during epilepsy, which may act by attenuating neuroinflammation through the suppression of NF-κB activation and iNOS production. At appropriate doses, miconazole may be a safe and effective approved drug that can easily be repositioned for clinical practice.
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http://dx.doi.org/10.1016/j.nbd.2022.105823DOI Listing
July 2022

Exploring the genetic relationship between deep vein thrombosis and plasma protein: a new research idea.

Expert Rev Hematol 2022 Aug 1:1-7. Epub 2022 Aug 1.

Department of Joint Surgery, HongHui Hospital, Xi'an Jiaotong University, Xi'an, Shanxi, China.

Background: The aim of this article is to scan and analyze the genetic correlation between plasma proteome and deep venous thrombosis (DVT), and to explore the correlation between plasma protein and DVT.

Research Design And Methods: GWAS data of DVT and plasma proteins were analyzed with linkage disequilibrium scores, and plasma proteins that were genetically associated with DVT were screened out. To ascertain the causal link between potential plasma proteins and DVT, a Mendelian randomized (MR) study was used. This study used STRING to examine the pathogenesis of DVT in connection with the gene encoding plasma protein.

Results: Several suggestive plasma proteins were detected for DVT, such as Complement factor B (P value=0.0177), Chromogranin-A (P value=0.0158). Through MR analysis, we found that there was a significant positive causal relationship between Chromogranin-A (exposure) and DVT(outcome) (β=-0.0117, P<0.0001). Our STRING analysis revealed that hsa04610 was associated with coagulation cascade in the KEGG pathway of Complement factor B(P<0.0001), which was based on GO and KEGG analysis of 8 selected plasma proteins.

Conclusions: A genetic link between plasma protein and DVT was thoroughly investigated. Our findings provide a fresh perspective on the genetics and pathogenesis of DVT.
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http://dx.doi.org/10.1080/17474086.2022.2104707DOI Listing
August 2022

Optimal Number of Clusters by Measuring Similarity Among Topographies for Spatio-Temporal ERP Analysis.

Brain Topogr 2022 Jul 18. Epub 2022 Jul 18.

School of Biomedical Engineering, Faculty of Electronic and Electrical Engineering, Dalian University of Technology, Dalian, China.

Averaging amplitudes over consecutive time samples (i.e., time window) is widely used to calculate the peak amplitude of event-related potentials (ERPs). Cluster analysis of the spatio-temporal ERP data is a promising tool to determine the time window of an ERP of interest. However, determining an appropriate number of clusters to optimally represent ERPs is still challenging. Here, we develop a new method to estimate the optimal number of clusters utilizing consensus clustering. Various polarity dependent clustering methods, namely, k-means, hierarchical clustering, fuzzy c-means, self-organizing map, spectral clustering, and Gaussian mixture model, are used to configure consensus clustering after assessing them individually. When a range of clusters is applied many times, the optimal number of clusters should correspond to the expectation, which is the average of the obtained mean inner-similarities of estimated time windows across all conditions and groups converge in the satisfactory thresholds. In order to assess our method, the proposed method has been applied to simulated data and prospective memory experiment ERP data aimed to qualify N2 and P3, and N300 and prospective positivity components, respectively. The results of determining the optimal number of clusters meet at six cluster maps for both ERP data. In addition, our results revealed that the proposed method could be reliably applied to ERP data to determine the appropriate time window for the ERP of interest when the measurement interval is not accurately defined.
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http://dx.doi.org/10.1007/s10548-022-00903-2DOI Listing
July 2022

Annotation-guided encoder-decoder network for bone extraction in ultrasound-assisted orthopedic surgery.

Comput Biol Med 2022 Jul 14;148:105813. Epub 2022 Jul 14.

Department of Orthopaedics, Beijing Tsinghua Changgung Hospital, Tsinghua University, China.

The patients and surgeons are usually exposed in massive ionizing radiation during fluoroscopy-based navigation orthopedic surgery. Comparatively, ultrasound-assisted orthopedic surgery could not only decrease the risk of radiation but also provide rich navigation information. However, due to the artifacts in ultrasound images, the extraction of bone structure from ultrasound sequences can be a particularly difficult task, which leads to some major challenges in ultrasound-assisted orthopedic navigation. In this paper, we propose an annotation-guided encoder-decoder network (AGN) to extract bone structure from the radiation-free ultrasound sequences. Specifically, the variability of the ultrasound probe's pose leads to the change of the ultrasound frame during the acquisition of ultrasound sequences. Therefore, a feature alignment module deployed in the AGN model is used to achieve reliable matching across ultrasound frames. Moreover, inspired by the interactive ultrasound analysis, where user annotated foreground information can help target extraction, our AGN model incorporates the annotation information obtained by Siamese networks. Experimental results validated that the AGN model not only produced better bone surface extraction than state-of-the-art methods (IOU: 0.92 versus. 0.88), but also achieved almost real-time extraction with the speed about 15 frames per second. In addition, the acquired bone surface further provided radiation-free 3D intraoperative bone structure for the intuitive navigation of orthopedic surgery.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105813DOI Listing
July 2022

Recognition of the Multi-class Schizophrenia Based on the Resting-State EEG Network Topology.

Brain Topogr 2022 07 18;35(4):495-506. Epub 2022 Jul 18.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu, 611731, China.

The clinical therapy of schizophrenia (SCZ) replies on the corresponding accurate and reliable recognition. Although efforts have been paid, the diagnosis of SCZ is still roughly subjective, it is thus urgent to search for related objective physiological parameters. Motivated by the great potential of resting-state networks in underling the brain deficits among different SCZ groups, in this study, we then developed a multi-class feature extraction approach that could effectively extract the spatial network topology and facilitate the recognition of the SCZ, by combining a network structure based supervised learning with an ensemble co-decision strategy. The results demonstrated that the multi-class spatial pattern of the network (MSPN) features outperformed the other conventional electrophysiological features, such as relative power spectrums and network properties, and achieved the highest classification accuracy of 71.58% in the alpha band. These findings did validate that the resting-state MSPN is a promising tool for the clinical assessment of the SCZ.
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http://dx.doi.org/10.1007/s10548-022-00907-yDOI Listing
July 2022

Genome- and transcriptome-wide association studies show that pulmonary embolism is associated with bone-forming proteins.

Expert Rev Hematol 2022 Jul 18. Epub 2022 Jul 18.

Department of Joint Surgery, HongHui Hospital, Xi'an Jiaotong University, Xi'an, Shanxi, 710054, China.

Background: Pulmonary embolism (PE) is a leading cause of death in stroke patients and a severe health burden worldwide. There is a pressing need to understand the mechanisms by which it occurs and to identify at-risk patients efficiently and accurately.

Objectives: The aim of this paper was to analyze the genetic correlation between PE and human plasma proteins through genome-wide association study (GWAS) with transcriptome-wide association study (TWAS), in combination with mRNA expression profiling at three levels: DNA, RNA, and protein.

Methods: First, based on data from GWAS in European populations, we performed a linkage disequilibrium score regression (LDSC) analysis of plasma proteins and PE in 3,283 individuals and additionally analyzed the genetic association between PE and fracture. Then, we performed a TWAS on PE GWAS data using skeletal muscle and blood for gene expression references. Finally, we validated the genetic correlation between PE and human plasma proteins by co-matching the genes encoding the identified proteins and those identified using TWAS with the differentially expressed genes obtained from mRNA expression profiling of PE (Figure1).

Results: We identified five plasma proteins associated with PE, including hydroxycarboxylic acid receptor 2, defensin 118, and bone morphogenetic protein (BMP) 7, as well as a relationship between PE and fracture. Comparison of genes encoding these proteins with genes obtained from TWAS and then with differentially expressed genes obtained from PE mRNA expression profiling revealed that PE was highly correlated with the BMP family of genes.
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http://dx.doi.org/10.1080/17474086.2022.2103534DOI Listing
July 2022

Case Report: Extracorporeal Membrane Oxygenation Followed by Intra-Aortic Balloon Counterpulsation Successfully Treated Cardiac Arrest Caused by Anomalous Origin of a Left Coronary Artery From the Right Coronary Sinus.

Front Med (Lausanne) 2022 29;9:936721. Epub 2022 Jun 29.

Department of Intensive Care Medicine, Northern Jiangsu People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, China.

Background: Anomalous origin of a coronary artery (AOCA) is defined as the failure of the coronary artery to originate from the normal coronary sinus. The anomalous origin of the left coronary artery arising from the right coronary sinus is rare, dangerous and at risk of malignant arrhythmia, sudden death, and high mortality.

Case Presentation: In this study, we present a 14-year-old adolescent male who went to a hospital with transient unconsciousness after exercise, who subsequently developed cardio arrest due to malignant arrhythmia. He was admitted to the intensive care unit, and who subsequently received successful veno-arterial extracorporeal membrane oxygenation (VA ECMO) assisted circulation followed by intra-aortic balloon counterpulsation (IABP). Echocardiography and cardiac CTA were also performed, further confirming that the abnormal left coronary artery originated from the right coronary sinus. The patient subsequently underwent heart surgery.

Conclusion: The successful treatment of the patient in this report was attributed to the immediately VA ECMO, supplemented by IABP. Establishing clear diagnosis is a process of multidisciplinary joint diagnosis, which provides a reference for clinicians when encountering similar cases.
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http://dx.doi.org/10.3389/fmed.2022.936721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276961PMC
June 2022

The time-varying networks of the wrist extension in post-stroke hemiplegic patients.

Cogn Neurodyn 2022 Aug 2;16(4):757-766. Epub 2021 Nov 2.

Neurorehabilitation Laboratory, Department of Rehabilitation Medicine, Shenzhen Hospital, Southern Medical University, Shenzhen, 518100 China.

Hemiplegia is a common dysfunction caused by the brain stroke and leads to movement disability. Although the lateralization of movement-related potential, the event-related desynchronization, and more complicated inter-regional information coupling have been investigated, seldom studies have focused on investigating the dynamic information exchanging among multiple brain regions during motor execution for post-stroke hemiplegic patients. With high temporal-resolution electroencephalogram (EEG), the time-varying network is able to reflect the dynamical complex network modalities corresponding to the movements at a millisecond level. In our present study, the wrist extension experiment was designed, along with related EEG datasets being collected. Thereafter, the corresponding time-varying networks underlying the wrist extension were accordingly constructed by adopting the adaptive directed transfer function and then statistically explored, to further uncover the dynamic network deficits (i.e., motor dysfunction) in post-stroke hemiplegic patients. Results of this study found the effective connectivity between the stroked motor area and other areas decreased in patients when compared to healthy controls; on the contrary, the enhanced connectivity between non-stroked motor areas and other areas, especially the frontal and parietal-occipital lobes, were further identified for patients during their accomplishing the designed wrist extension, which might dynamically compensate for the deficited patients' motor behaviors. These findings not only helped deepen our knowledge of the mechanism underlying the patients' motor behaviors, but also facilitated the real-time strategies for clinical therapy of brain stroke, as well as providing a reliable biomarker to predict the future rehabilitation.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11571-021-09738-2.
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http://dx.doi.org/10.1007/s11571-021-09738-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279526PMC
August 2022

Quantification of aneurysm wall enhancement in intracranial fusiform aneurysms and related predictors based on high-resolution magnetic resonance imaging: a validation study.

Ther Adv Neurol Disord 2022 12;15:17562864221105342. Epub 2022 Jul 12.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, No. 119, South 4th Ring West Road, Fengtai District, Beijing 100070, China.

Background: Aneurysm wall enhancement (AWE) in high-resolution magnetic resonance imaging (HR-MRI) has emerged as a new imaging biomarker of intracranial aneurysm instability.

Objective: To determine a standard method of AWE quantification for predicting fusiform intracranial aneurysms (FIAs) stability by comparing the sensitivity of each parameter in identifying symptomatic FIAs. The predictors of AWE and FIA types were also identified.

Methods: We retrospectively analyzed consecutive fusiform aneurysm patients who underwent HR-MRI from two centers. The aneurysm-to-pituitary stalk contrast ratio (CR), aneurysm enhancement ratio, and aneurysm enhancement index were extracted, and their sensitivities in discriminating aneurysm symptoms were compared using the receiver-operating characteristic curve. Morphological parameters of fusiform aneurysm were extracted based on 3D vessel model. Uni- and multivariate analyses of related predictors for AWE, CR, and FIA types were performed, respectively.

Results: Overall, 117 patients (mean age, 53.3 ± 11.7 years; male, 75.2%) with 117 FIAs underwent HR-MRI were included. CR with the maximum signal intensity (CR) had the highest sensitivity in identifying symptomatic FIAs with an area under the curve value (0.697) and a cut-off value of 0.90. The independent predictors of AWE were aneurysm symptoms [(odds ratio) OR = 3.754,  = 0.003], aspirin use (OR = 0.248,  = 0.037), and the maximum diameter of the cross-section (OR = 1.171,  = 0.043). The independent predictors of CR were aneurysm symptoms (OR = 1.289,  = 0.003) and posterior circulation aneurysm (OR = 1.314,  = 0.001). Transitional-type showed higher rates of hypertension and mural thrombus over both dolichoectatic- and fusiform-type FIAs.

Conclusion: CR may be the most reliable parameter to quantify AWE to distinguish symptomatic FIAs. It also has the potential to identify unstable FIAs. Several factors contribute to the complex pathophysiology of FIAs and need further validation in a larger cohort.
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http://dx.doi.org/10.1177/17562864221105342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280813PMC
July 2022

Next-Generation Metagenome Sequencing Shows Superior Diagnostic Performance in Acid-Fast Staining Sputum Smear-Negative Pulmonary Tuberculosis and Non-tuberculous Mycobacterial Pulmonary Disease.

Front Microbiol 2022 1;13:898195. Epub 2022 Jul 1.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin, China.

In this study, we explored the clinical value of next-generation metagenome sequencing (mNGS) using bronchoalveolar lavage fluid (BALF) samples from patients with acid-fast staining (AFS) sputum smear-negative pulmonary tuberculosis (PTB) and non-tuberculous mycobacterial pulmonary disease (NTM-PD). Data corresponding to hospitalized patients with pulmonary infection admitted to the hospital between July 2018 and July 2021, who were finally diagnosed with AFS sputum smear-negative PTB and NTM-PD, were retrospectively analyzed. Bronchoscopy data as well as mNGS, Xpert, AFS (BALF analysis), and T-SPOT (blood) data, were extracted from medical records. Thereafter, the diagnostic performances of these methods with respect to PTB and NTM-PD were compared. Seventy-one patients with PTB and 23 with NTM-PD were included in the study. The sensitivities of mNGS, Xpert, T-SPOT, and AFS for the diagnosis of PTB were 94.4% (67/71), 85.9% (61/71), 64.8% (46/71), and 28.2% (20/71), respectively, and the diagnostic sensitivity of mNGS combined with Xpert was the highest (97.2%, 67/71). The specificity of Xpert was 100%, while those of AFS and T-SPOT were 73.9% (17/23) and 91.3% (21/23), respectively. Further, the 23 patients with NTM-PD could be identified using mNGS, and in the population with immunosuppression, the sensitivities of mNGS, Xpert, T-SPOT, and AFS were 93.5% (29/31), 80.6% (25/31), 48.4% (15/31), and 32.3% (10/31), respectively, and the diagnostic sensitivity of mNGS combined with Xpert was the highest (100%, 31/31). The specificities of Xpert and T-SPOT in this regard were both 100%, while that of AFS was 40% (2/5). Furthermore, using mNGS, all the NTM samples could be identified. Thus, the analysis of BALF samples using mNGS has a high accuracy in the differential diagnosis of MTB and NTM. Further, mNGS combined with Xpert can improve the detection of MTB, especially in AFS sputum smear-negative samples from patients with compromised immune states or poor responses to empirical antibiotics.
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http://dx.doi.org/10.3389/fmicb.2022.898195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283093PMC
July 2022

Logistics Distribution Route Optimization Based on Genetic Algorithm.

Comput Intell Neurosci 2022 6;2022:8468438. Epub 2022 Jul 6.

School of Humanities and Management, Southwest Medical University, Luzhou 646000, China.

Aiming at the problem of logistic division based on genetic algorithm, it is planned to study the improvement of logistic distribution methods. We first meet the requirements of the genetic algorithm of logistic development, use the division method to divide the delivery area of the gene, and formulate a functional delivery plan, which generally includes weight measurement, measurement time, customer value measurement, instrument measurement time, and the whole process index. We set weight goals and find the best way to improve genetic algorithm delivery. The experimental comparison results show that the optimal method takes less than 2 minutes to find the optimal method, while the normal process takes 4 minutes to find the optimal method, and the longest can reach 5 minutes. The comparison shows that the traditional algorithm takes longer to find the correct way than the algorithm developed this time. Finally, the simple logistic distribution optimization method model and the soft time-limited logistic distribution processing optimization model are calculated and simulated by the genetic testing algorithm and genetic algorithm development. The effectiveness of the improved genetic algorithm in local research and the effectiveness of the logistic transportation allocation solution are determined.
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http://dx.doi.org/10.1155/2022/8468438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279064PMC
July 2022

Biocrust diazotrophs and bacteria rather than fungi are sensitive to chronic low N deposition.

Environ Microbiol 2022 Jul 18. Epub 2022 Jul 18.

State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.

Anthropogenic long-term nitrogen (N) deposition may dramatically impact biocrusts due to the overarching N limitation of soil biota in deserts. Even low levels of N may reach a critical loading threshold altering biocrust constituents and function. To identify the impact of chronic and continuous low levels of N deposition on biocrusts, we created a realistic gradient mirroring anthropogenic N addition rate (2:1 NH : NO rates: 0.3, 0.5, 1.0, 1.5, 3 g N m  yr ) and measured the response of bacteria and fungi within cyanobacterial-dominated biocrusts over 8 years in a temperate desert, the Gurbantunggut Desert, China. We found that once N deposition reached 1.5 g N m  yr biocrust bacterial communities, including diazotrophs, were altered while no such tipping point existed for fungi. Above the threshold, bacterial richness was enhanced, the relative abundance of Chloroflexi, FBP and Gemmatimonadetes was elevated, and diazotrophs shifted from being dominated by Nostocaceae and Scytonemataceae (Cyanobacteria) to free-living Bradyrhizobiaceae (Alphaproteobacteria). Alternatively, the relative recovery of a few fungal species within the Lecanorales, Pleosporales and Verrucariales became either enriched or diminished due to N deposition. The chronic addition of N resulted in a dense and interconnected bacterial co-occurrence network that accentuated a functional shift from networks dominated by phototrophic species within the Nostocaceae, Xenococcaceae, Phormidiaceae and Scytonemataceae (Cyanobacteria) to ammonia-oxidizing species within the Nitrosomonadaceae (Betaproteobacteria) and nitrifying bacteria [i.e. Nitrospiraceae (Nitrospirae)]. Based on structural equation models, the effects of N additions on biocrust constituents were imposed through indirect effects on pH, soil electrical conductivity and ammonium concentrations. In summary, biocrust constituents are generally insensitive to chronic low levels of N depositions until rates reach above 1.5 g N m  yr with diazotrophs being the most sensitive biocrust constituents followed by bacteria and finally fungi. Ultimately once the threshold is reached N deposition favours biocrust constituents utilizing inorganic N and other C sources over relying on phototrophic and/or N-fixing cyanobacteria for C and N.
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http://dx.doi.org/10.1111/1462-2920.16095DOI Listing
July 2022
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