Publications by authors named "Peng Wu"

1,238 Publications

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RNA-binding protein CELF1 promotes cardiac hypertrophy via interaction with PEBP1 in cardiomyocytes.

Cell Tissue Res 2021 Oct 20. Epub 2021 Oct 20.

Heart Center, Tianjin Third Central Hospital, 83 Jintang Road, Tianjin, 300170, China.

Cardiac hypertrophy is considered as a common pathophysiological process in various cardiovascular diseases. CUG triplet repeat-binding protein 1 (CELF1) is an RNA-binding protein that has been shown to be an important post-transcription regulator and involved in several types of cancer, whereas its role in cardiac remodeling remains unclear. Herein, we found that the expression of CELF1 was significantly increased in pressure overload-induced hypertrophic hearts and angiotensin II (Ang II)-induced neonatal cardiomyocytes. Based on transverse aortic constriction-induced cardiac hypertrophy model, CELF1 deficiency markedly ameliorated cardiac hypertrophy, cardiac fibrosis, oxidative stress, and apoptosis. Accordingly, CELF1 deficiency alleviated the production of reactive oxygen species (ROS) and apoptosis of neonatal cardiomyocytes via inhibition of Raf1, TAK1, ERK1/2, and p38 phosphorylation. Mechanistically, depletion or overexpression of CELF1 negatively regulated the protein expression of phosphatidylethanolamine-binding protein 1 (PEBP1), while the mRNA expression of PEBP1 remained unchanged. RNA immunoprecipitation revealed that CELF1 directly interacted with PEBP1 mRNA. Biotin pull-down analysis and dual-luciferase assay showed that CELF1 directly bound to the fragment 1 within 3'UTR of PEBP1. Moreover, knockdown of PEBP1 partially enhanced the production of ROS and apoptosis of neonatal cardiomyocytes inhibited by CELF1 deficiency. In conclusion, CELF1 binds to the 3'UTR of PEBP1 and acts as an endogenous activator of MAPK signaling pathway. Inhibition of CELF1 attenuates pathological cardiac hypertrophy, oxidative stress, and apoptosis, thus could be a potential therapeutic strategy of pathological cardiac hypertrophy.
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http://dx.doi.org/10.1007/s00441-021-03541-5DOI Listing
October 2021

Integrated study on the comprehensive magnetic-field configuration performance in the 150 kW superconducting magnetoplasmadynamic thruster.

Sci Rep 2021 Oct 19;11(1):20706. Epub 2021 Oct 19.

Beijing Institute of Control Engineering, Beijing, 100080, China.

Higher magnetic fields are always favoured in the magnetoplasmadynamic thruster (MPDT) due to its superior control of the plasma profile and acceleration process. This paper introduces the world's first integrated study on the 150 kW level AF-MPDT equipped with a superconductive coil. A completely new way of using superconducting magnet technology to confine plasma with high energy and extremely high temperatures is proposed. Using the PIC method of microscopic particle simulation, the plasma magnetic nozzle effect and performance of the MPDT under different magnetic-field conditions were studied. The integrated experiment used demonstrated that, in conjunction with the superconducting coil, greater homogeneity and a stronger magnetic field not only caused more even cathode ablation and improved its lifespan but also improved the performance of the MPDT (maximum thrust was 4 N at 150 kW, 0.56 T). Maximum thrust efficiency reached 76.6% and the specific impulse reached 5714 s.
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http://dx.doi.org/10.1038/s41598-021-00308-4DOI Listing
October 2021

Recent advances in utilization of pectins in biomedical applications: a review focusing on molecular structure-directing health-promoting properties.

Crit Rev Food Sci Nutr 2021 Oct 12:1-34. Epub 2021 Oct 12.

College of Food Science and Engineering, Shandong Agricultural University, Taian, China.

The numerous health benefits of pectins justify their inclusion in human diets and biomedical products. This review provides an overview of pectin extraction and modification methods, their physico-chemical characteristics, health-promoting properties, and pharmaceutical/biomedical applications. Pectins, as readily available and versatile biomolecules, can be tailored to possess specific functionalities for food, pharmaceutical and biomedical applications, through judicious selection of appropriate extraction and modification technologies/processes based on green chemistry principles. Pectin's structural and physicochemical characteristics dictate their effects on digestion and bioavailability of nutrients, as well as health-promoting properties including anticancer, immunomodulatory, anti-inflammatory, intestinal microflora-regulating, immune barrier-strengthening, hypercholesterolemia-/arteriosclerosis-preventing, anti-diabetic, anti-obesity, antitussive, analgesic, anticoagulant, and wound healing effects. HG, RG-I, RG-II, molecular weight, side chain pattern, and degrees of methylation, acetylation, amidation and branching are critical structural elements responsible for optimizing these health benefits. The physicochemical characteristics, health functionalities, biocompatibility and biodegradability of pectins enable the construction of pectin-based composites with distinct properties for targeted applications in bioactive/drug delivery, edible films/coatings, nano-/micro-encapsulation, wound dressings and biological tissue engineering. Achieving beneficial synergies among the green extraction and modification processes during pectin production, and between pectin and other composite components in biomedical products, should be key foci for future research.
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http://dx.doi.org/10.1080/10408398.2021.1988897DOI Listing
October 2021

A Hydrothermally Stable Single-Atom Catalyst of Pt Supported on High-Entropy Oxide/AlO: Structural Optimization and Enhanced Catalytic Activity.

ACS Appl Mater Interfaces 2021 Oct 11;13(41):48764-48773. Epub 2021 Oct 11.

Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.

A catalyst with high-entropy oxide (HEO)-stabilized single-atom Pt can afford low-temperature activity for catalytic oxidation and remarkable durability even under harsh conditions. However, HEO is easy to harden during sintering, which results in a few defective sites for anchoring single-atom metals. Herein, we present a sol-gel-assisted mechanical milling strategy to achieve a single-atom catalyst of Pt-HEO/AlO. The strong interaction between HEO and AlO effectively inhibits the growth of HEO microparticles, which leads to generation of more surface defects because of the nanoscale effect. Meanwhile, another strong interaction between Pt and HEO stabilizes single-atom Pt on HEO. Temperature-programmed techniques further verify that the reactivity of surface lattice oxygen species is enhanced because of the Pt-O-M bonds on the surface of HEO. Unlike conventional single-atom Pt catalysts, Pt-HEO/AlO as a heterogeneous catalyst not only exhibits superior stability against hydrothermal aging but also presents long-term reaction stability for CO catalytic oxidation, which exceeds 540 h. The present work opens a new door for rational design of hydrothermally stable single-atom Pt catalysts, which are highly promising in practical applications.
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http://dx.doi.org/10.1021/acsami.1c14456DOI Listing
October 2021

Attribute Augmented Network Embedding Based on Generative Adversarial Nets.

IEEE Trans Neural Netw Learn Syst 2021 Oct 8;PP. Epub 2021 Oct 8.

Network embedding is to learn low-dimensional representations of nodes while preserving necessary information for network analysis tasks. Though representations preserving both structure and attribute features have achieved in many real-world applications, learning these representations for networks with attribute information is difficult due to the heterogeneity between structure and attribute information. Many existing methods have been proposed to preserve explicit proximities between nodes, with optimization limited to node pairs with large structure and attribute proximities, which may lead to overfitting. To address the above problems, we adopt an attribute augmented network to represent attribute and structure information in a unified framework. Specifically, we study the problem of attribute augmented network embedding that exploits the strength of generative adversarial nets (ANGANs) in capturing the latent distribution of data to learn robust and informative representations of nodes. The ANGAN method obtains the low-dimensional representations of nodes through adversarial learning between the generative and discriminative models. The generative model approximates the underlying connectivity and attributes distributions of nodes by using the distributions generated from the learned representations. It is implemented by utilizing the properties of the attribute augmented network to improve the traditional Skip-gram model. The discriminative model is designed as a binary classifier to distinguish the truly connected node pairs from the generated ones. The pre-training algorithm and the teacher forcing approach are adopted to improve training efficiency and stability. Empirical results show that ANGAN generally outperforms state-of-the-art methods in various real-world applications, which demonstrates the effectiveness and generality of our method.
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http://dx.doi.org/10.1109/TNNLS.2021.3116419DOI Listing
October 2021

LINC00355 induces gastric cancer proliferation and invasion through promoting ubiquitination of P53.

Cell Death Discov 2020 Oct 8;6(1):99. Epub 2020 Oct 8.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanjing Medical University, 211166, Nanjing, People's Republic of China.

Long noncoding RNAs (LncRNAs) have been reported to play critical roles in gastric cancer, but true biomarkers remain unknown. In this study, we found a new lncRNA LINC00355 that was involved in malignant progression of gastric cancer (GC) and further revealed its role and mechanism. Differentially expressed lncRNAs were identified through bioinformatics, and qRT-PCR was used to validate the expression of LINC00355 in gastric cancer tissues and cells. The biological role of LINC00355 in GC was detected by gene overexpression and knockdown experiments. Subcellular fractionation, qRT-PCR, and FISH were performed to detect the subcellular localization. Co-IP and western blotting were used to study the ubiquitination-mediated regulation of P53 and the expression of the E3 ligases RAD18 and UBE3C. The results showed that LINC00355 was significantly increased in gastric cancer cell lines and patient tissues and closely correlated with late stages, distant metastasis, and poor prognosis of patients. High expression of LINC00355 promoted the proliferation and invasion of gastric cancer cells in vivo and in vitro. Mechanistic studies found that LINC00355 that mainly located in the nucleus, acting as a transcriptional activator, promoted transcription of RAD18 and UBE3C, which both bind to P53 and mediate the ubiquitination and degradation of P53. Furthermore, LINC00355 overexpression enhanced the ubiquitination process, and LINC00355 knockdown alleviated it. These results indicated that LINC00355 induces gastric cancer cell proliferation and invasion by promoting transcription of RAD18 and UBE3C, which mediates ubiquitination of P53 and thereby plays a critical role in survival and tumorigenicity of gastric cancer cells. LINC00355 may represent a new mechanism for GC progression and provide a potential marker for GC diagnosis and treatment.
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http://dx.doi.org/10.1038/s41420-020-00332-9DOI Listing
October 2020

Total hip revision with custom-made spacer and prosthesis: A case report.

World J Clin Cases 2021 Sep;9(25):7605-7613

Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China.

Background: Both periprosthetic joint infections (PJIs) and severe femoral segmental defects are catastrophic complications of total hip arthroplasty (THA), and both present a significant challenge in revisional surgery. There are limited data available to guide clinical decision making when both occur concurrently.

Case Summary: A 61-year-old woman presented with a 6-mo history of a sinus tract at the site of her original THA incision. Radiological imaging revealed a total hip joint implant with an ipsilateral segmental femoral defect. Based on histological, radiological, laboratory, and clinical features, a diagnosis of concurrent chronic PJI and segmental femoral defect (Type IIIB, Paprosky classification) was made. After multidisciplinary team discussion, three-dimensional (3D)-printed, custom-made antibiotic spacers were created that could be used to mold antibiotic-loaded cement spacer. These were placed following PJI debridement in the first stage of revision surgery. After the PJI was eliminated, a 3D-printed, custom-made, femoral prosthesis was created to repair the considerable femoral defect. After 20-mo follow-up, the patient had excellent functional outcomes with a near-normal range of hip movement. So far, neither evidence of recurrent infection nor loosening of the prosthesis has been observed.

Conclusion: We describe a case of "two-stage, custom-made" total hip revision to treat PJI with a concurrent segmental femoral defect. Use of a personalized, 3D-printed spacer and proximal femoral prosthesis led to satisfactory hip function and no early postoperative complications. Use of a customized implant provides surgeons with an alternative option for patients where no suitable spacer or implant is available. However, the long-term function, longevity, and cost-effectiveness of the use of custom-made prostheses have yet to be fully explored.
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http://dx.doi.org/10.12998/wjcc.v9.i25.7605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464474PMC
September 2021

Revamping Lithium-Sulfur Batteries for High Cell-Level Energy Density by Synergistic Utilization of Polysulfide Additives and Artificial Solid-Electrolyte Interphase Layers.

Adv Mater 2021 Oct 4:e2104246. Epub 2021 Oct 4.

Department of Materials Science and Engineering, Texas A&M University, College Station, TX, 77843, USA.

Despite the high theoretical capacity of lithium-sulfur (Li-S) batteries, a high cell-level energy density and a long cycling life are barely achieved, mainly due to the large electrolyte-to-sulfur ratio, polysulfide (PS) shuttle causing the loss of active sulfur, and the formation of passivation layers on the Li anode. To raise the energy density, holding PS in the cathode has been the most popular approach. Still, it has failed, particularly, when the sulfur loading is high enough to have energy densities similar to those of commercial Li-ion batteries. Here, a practical approach of achieving high "cell-level" energy densities is attempted using lithium PS (LPS)-containing electrolytes instead of a pure electrolyte, reducing the electrolyte-to-sulfur ratio and PS diffusion out of the cathode due to concentration differences. Meanwhile, the persistent problems including PS passivation and Li dendrites are suppressed using Li S-phobic artificial solid-electrolyte interphase (A-SEI) layers on Li metal. The synergistic effects from the LPS additives and A-SEI result in a superior cell-level volumetric energy density of 650 Wh L as well as large cumulative energy densities considering cycling life. This approach provides an important stepping stone to realize commercial Li-S batteries rivaling the current Li-ion batteries.
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http://dx.doi.org/10.1002/adma.202104246DOI Listing
October 2021

A laminar augmented cascading flexible neural forest model for classification of cancer subtypes based on gene expression data.

BMC Bioinformatics 2021 Oct 2;22(1):475. Epub 2021 Oct 2.

School of Information Science and Engineering, University of Jinan, Jinan, China.

Background: Correctly classifying the subtypes of cancer is of great significance for the in-depth study of cancer pathogenesis and the realization of personalized treatment for cancer patients. In recent years, classification of cancer subtypes using deep neural networks and gene expression data has gradually become a research hotspot. However, most classifiers may face overfitting and low classification accuracy when dealing with small sample size and high-dimensional biology data.

Results: In this paper, a laminar augmented cascading flexible neural forest (LACFNForest) model was proposed to complete the classification of cancer subtypes. This model is a cascading flexible neural forest using deep flexible neural forest (DFNForest) as the base classifier. A hierarchical broadening ensemble method was proposed, which ensures the robustness of classification results and avoids the waste of model structure and function as much as possible. We also introduced an output judgment mechanism to each layer of the forest to reduce the computational complexity of the model. The deep neural forest was extended to the densely connected deep neural forest to improve the prediction results. The experiments on RNA-seq gene expression data showed that LACFNForest has better performance in the classification of cancer subtypes compared to the conventional methods.

Conclusion: The LACFNForest model effectively improves the accuracy of cancer subtype classification with good robustness. It provides a new approach for the ensemble learning of classifiers in terms of structural design.
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http://dx.doi.org/10.1186/s12859-021-04391-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487515PMC
October 2021

Vaccination Uncertainties and COVID-19 Prospects in 2021.

China CDC Wkly 2021 Feb;3(7):150-152

WHO Collaborating Centre for Infectious Disease Epidemiology and Control, School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

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http://dx.doi.org/10.46234/ccdcw2021.044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392924PMC
February 2021

Comparison of the Use of Magnetic Resonance Imaging of Partial Anterior Cruciate Ligament Tears Using Maximum Knee Flexion in the Lateral Decubitus Position with Routine Knee Positioning.

Med Sci Monit 2021 Oct 1;27:e932228. Epub 2021 Oct 1.

Department of Orthopeadics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China (mainland).

BACKGROUND This study assessed magnetic resonance imaging (MRI) of acute and chronic partial anterior cruciate ligament (ACL) tears using maximum knee flexion in the lateral decubitus position compared with routine knee positioning in 204 patients at a single center. MATERIAL AND METHODS Based on the time interval from injury to MRI examination, the 204 patients in this study were divided into 3 groups: subacute (6 weeks to 3 months), intermediate (3 months to 1 year), and chronic (>1 year). All patients received both routine MRI (MRI R) and maximum knee flexion in the lateral decubitus position MRI (MRI S) examination, followed by knee arthroscopy. Three radiologists blinded to patient groups evaluated the MRI scans and made a diagnosis. Results of knee arthroscopy were referenced as the criterion standard. The sensitivity and specificity of MRI R and MRI S groups were calculated and compared. RESULTS The MRI S diagnostic rate was comparable to that of knee arthroscopy. MRI S had significantly higher sensitivity than MRI R for partial ACL tears, especially in the intermediate group (P<0.01). CONCLUSIONS MRI of partial ACL tears using maximum knee flexion in the lateral decubitus position improved the diagnostic rate relative to routine MRI examination, particularly in patients in the intermediate group.
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http://dx.doi.org/10.12659/MSM.932228DOI Listing
October 2021

Polyetheretherketone versus titanium CAD-CAM framework for implant-supported fixed complete dentures: a retrospective study with up to 5-year follow-up.

J Prosthodont Res 2021 Sep 30. Epub 2021 Sep 30.

National Clinical Research Center for Oral Diseases and State Key Laboratory of Military Stomatology and Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.

Purpose: To evaluate the performance of polyetheretherketone (PEEK) versus titanium computer-aided designed and manufactured (CAD-CAM) framework for implant-supported fixed complete dentures (ISFCDs) with a follow-up for a duration of up to 5 years.

Methods: Consecutively edentulous patients who underwent ISFCDs with a PEEK framework or titanium framework at one dental specialist center were included in this retrospective study. Implant/prosthesis survival rates, mechanical/biological complications, and bone and soft tissue parameters were analyzed. Overall survival was analyzed using Kaplan-Meier survival curves and the log-rank test.

Results: Sixty ISFCDs (29 PEEK, 31 titanium) performed on 43 edentulous patients (331 implants) were included. An implant survival rate of 100% was obtained. There was no significant difference in the cumulative prosthesis survival rate between the PEEK (93.1%) and titanium groups (93.5%). The most common mechanical complications were fracture of the artificial veneer in both the PEEK (13.8%) and titanium (16.7%) groups. Bruxers had a higher prevalence of mechanical complications than non-bruxers (p<0.05). The biological complications included bleeding upon probing (13.8% for the PEEK group; 16.1% for the titanium group), soft tissue inflammation (3.4% for the PEEK group; 3.2% for the titanium group), and temporomandibular disorders (6.5% for the titanium group). The vertical bone loss was significantly lower in the PEEK group (0.70 mm) than in the titanium group (0.96 mm). Smokers had a significantly higher prevalence of biological complications than non-smokers.

Conclusions: Within the limitations of this study, ISFCDs with PEEK frameworks can provide good prognosis for edentulous patients, still requiring longer-term validation.
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http://dx.doi.org/10.2186/jpr.JPR_D_20_00142DOI Listing
September 2021

RNA N -methyladenosine modification in the lethal teamwork of cancer stem cells and the tumor immune microenvironment: Current landscape and therapeutic potential.

Clin Transl Med 2021 Sep;11(9):e525

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

N -methyladenosine (m A), the newest and most prevalent layer of internal epigenetic modification in eukaryotic mRNA, has been demonstrated to play a critical role in cancer biology. Increasing evidence has highlighted that the interaction between cancer stem cells (CSCs) and the tumor immune microenvironment (TIME) is the root cause of tumorigenesis, metastasis, therapy resistance, and recurrence. In recent studies, the m A modification has been tightly linked to this CSC-TIME interplay, participating in the regulation of CSCs and TIME remolding. Interestingly, the m A modification has also been identified as a novel decisive factor in the efficacy of immunotherapies-particularly anti-PD-1/PD-L1 monotherapies-by changing the plasticity of the TIME. Given the functional importance of the m A modification in the crosstalk between CSCs and the TIME, targeting m A regulators will open new avenues to overcome therapeutic resistance, especially for immune checkpoint-based immunotherapy. In the present review, we summarize the current landscape of m A modifications in CSCs and the TIME, and also prospect the underling role of m A modifications at the crossroads of CSCs and the TIME for the first time. Additionally, to provide the possibility of modulating m A modifications as an emerging therapeutic strategy, we also explore the burgeoning inhibitors and technologies targeting m A regulators. Lastly, considering recent advances in m A-seq technologies and cancer drug development, we propose the future directions of m A modification in clinical applications, which may not only help to improve individualized monitoring and therapy but also provide enhanced and durable responses in patients with insensitive tumors.
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http://dx.doi.org/10.1002/ctm2.525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473646PMC
September 2021

Development of pH-activatable fluorescent probes for rapid visualization of metastatic tumours and fluorescence-guided surgery topical spraying.

Chem Commun (Camb) 2021 Oct 12;57(81):10636-10639. Epub 2021 Oct 12.

Research Center for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China.

A series of pH-activatable aza-BODIPY-based fluorescent probes were developed for rapid cancer visualization and real-time fluorescence-guided surgery by harnessing topical spraying. These probes exhibited good water-solubility, a tunable p from 5.0 to 7.9, and stable intense NIR emission at ∼725 nm under acidic conditions. AzaB5 with a p value of 6.7 was able to rapidly and clearly visualize pulmonary and abdominal metastatic tumours including tiny metastases less than 2 mm topical spraying, further improving intraoperative fluorescence-guided resection. We believe that AzaB5 is promising as a powerful tool to rapidly delineate a broad range of malignancies and assist surgical tumour resection.
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http://dx.doi.org/10.1039/d1cc04408gDOI Listing
October 2021

Treatment of spinal tuberculosis in rabbits using bovine serum albumin nanoparticles loaded with isoniazid and rifampicin.

Neurol Res 2021 Sep 28:1-7. Epub 2021 Sep 28.

Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, China.

Objective: To evaluate the clinical efficacy of bovine serum albumin nanoparticles loaded with isoniazid and rifampicin (INH-RFP-BSA-NPs) in the treatment of spinal tuberculosis in rabbits.

Methods: 35 spinal tuberculosis rabbit models were grouped into three groups, including 14 in group A and group B respectively and 7 in group C.All rabbits in group A were treated by INH-RFP-BSA-NPs's injection and in group B were treated with classic dosage form of INH and RFP, while in group C normal saline was given as the blank control. After intervention, the body weighing and CT scan, as well as concentration's measurement of INH and RFP in blood and tissues, were performed in all rabbits at the time of the 6thweek and 12th week, respectively.

Results: In group A, rabbits' weight increased by 0.44 kg and 0.27 kg within 6 weeks and 12 weeks' treatment respectively. The bactericidal concentrations of 1.64 µg•g for INH and 21.36 µg•g for RFP were measured in focus vertebral body 6 weeks post-injection and six weeks later the concentrations of INH and RFP in vertebral body still maintained at the level of 1.96 µg•g and 22.35 µg•grespectively. After 12 weeks therapy, CT-scanned showed all the necrotic tissue was replaced by normal bone tissue. In group B, all rabbits had no significant increment of body weight and 4 rabbits had paralysis of hind leg. The concentrations of INH and RFP in vertebral body and focus were much lower than group A. CT-scanned showed the focus vertebral body was only partially repaired after 12 weeks' therapy.

Conclusion: The INH-RFP-BSA-NPs has the characteristics of sustained release in vivo and target biodistribution in focus vertebral body. Its therapeutic effect in rabbit spinal tuberculosis is much better than common INH and RFP.
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http://dx.doi.org/10.1080/01616412.2021.1979749DOI Listing
September 2021

The landscape of mA regulators in small cell lung cancer: molecular characteristics, immuno-oncology features, and clinical relevance.

Mol Cancer 2021 Sep 27;20(1):122. Epub 2021 Sep 27.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

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http://dx.doi.org/10.1186/s12943-021-01408-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474928PMC
September 2021

Exosomal Proteins and miRNAs as Mediators of Amyotrophic Lateral Sclerosis.

Front Cell Dev Biol 2021 10;9:718803. Epub 2021 Sep 10.

Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Recent advances in the neurobiology and neurogenerative diseases have attracted growing interest in exosomes and their ability to carry and propagate active biomolecules as a means to reprogram recipient cells. Alterations in exosomal protein content and nucleic acid profiles found in human biological fluids have been correlated with various diseases including amyotrophic lateral sclerosis (ALS). In ALS pathogenesis, these lipid-bound nanoscale vesicles have emerged as valuable candidates for diagnostic biomarkers. Moreover, their capacity to spread misfolded proteins and functional non-coding RNAs to interconnected neuronal cells make them putative mediators for the progressive motor degeneration found remarkably apparent in ALS. This review outlines current knowledge concerning the biogenesis, heterogeneity, and function of exosomes in the brain as well as a comprehensive probe of currently available literature on ALS-related exosomal proteins and microRNAs. Lastly, with the rapid development of employing nanoparticles for drug delivery, we explore the therapeutic potentials of exosomes as well as underlying limitations in current isolation and detection methodologies.
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http://dx.doi.org/10.3389/fcell.2021.718803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461026PMC
September 2021

Corrigendum: Low-Dose Total Body Irradiation Can Enhance Systemic Immune Related Response Induced by Hypo-Fractionated Radiation.

Front Immunol 2021 3;12:745787. Epub 2021 Sep 3.

Department of Oncology, The Affiliated Hospital of Southwest Medical University, Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou, China.

[This corrects the article DOI: 10.3389/fimmu.2019.00317.].
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http://dx.doi.org/10.3389/fimmu.2021.745787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448506PMC
September 2021

Interventional Efects of the Topical of "Sanse Powder" Essential Oils Nanoemulsion on Knee Osteoarthritis in Rats by Targeting the ERS/TXNIP/NLRP3 Signaling Axis.

Front Pharmacol 2021 2;12:739644. Epub 2021 Sep 2.

Department of Orthopedics, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Our recent research is dedicated to finding effective drugs for the treatment of knee osteoarthritis (KOA) from traditional Chinese medicine and trying to make full use of modern science and technology to uncover the mechanisms and targets behind them. Synovial inflammation is one of the key pathological features of KOA, and a growing number of researchers realize that early intervention of synovial inflammation may be able to reverse disease progression. The close association of traditional natural products with modern nanotechnology may be important for improving the anti-synovitis efficacy. The purpose of our research was to explore the anti-synovitis mechanism of NEs-SP-EO that might be associated with the ERS/TXNIP/NLRP3 signalling axis. Chemical composition of "Sanse Powder" essential oil (SP-EO) and NEs-SP-EO were analyzed by GC-MS. NEs-SP-EO were prepared and characterized by nanoparticle tracking analysis, polydispersity index (PDI), zeta potential (ZP), ultraviolet-visible spectroscopy, and transmission electronic microscopy. The CCK8 assay for cell viability of NEs-SP-EO was performed on fibroblast-like synovial cells (FLSs) and the inflammatory environment was stimulated by LPS to explore the therapeutic mechanisms . Experiments of NEs-SP-EO were performed in male SD rats. The GC-MS results showed that 30 compounds were present in SP-EO and 11 components of NEs-SP-EO were identified. The results also showed that the formulation of NEs-SP-EO exhibited suitable particle size, negative charge, and stable system. and vivo testing, NEs-SP-EO produced anti-synovitis efficacy by reduced the induction of the ERS/TXNIP/NLRP3 signaling axis as well as regulating the overproduction of IL-1β, IL-18. We have developed a new type of essential oil nanoemulsion from "Sanse Powder" and demonstrated that it can managing synovitis of KOA. Besides, we have initially explored the anti-inflammatory mechanism that may be related to the ERS/TXNIP/NLRP3 signaling axis.
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http://dx.doi.org/10.3389/fphar.2021.739644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443772PMC
September 2021

Mixed matrix of [email protected] hybrids for enrichment and determination of phenoxy carboxylic acids in water and vegetables.

Food Chem 2021 Sep 9;371:131090. Epub 2021 Sep 9.

College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, PR China. Electronic address:

A novel mixed matrix of [email protected] hybrid was firstly formed by coating of hexahedral cage structure MOF with lightweight porous COF, and applied in dispersive solid-phase extraction of the phenoxy carboxylic acids (PCAs) from water and vegetable samples. Combined with liquid chromatography-tandem mass spectrometry, an excellent method with low limits of detection (0.69-1.79 ng·L/0.002-0.006 ng·g), good reproducibility (1.32%-7.02%/1.81%-6.71%), and excellent linearities (10-1000 ng·L, R ≥ 0.9955/0.04-50 ng·g, R ≥ 0.9966) was established. The adsorption mechanisms deduced that the π-π interaction, hydrophobic effects, hydrogen bond, and halogen bond may promote the excellent adsorption of the PCAs. Finally, the applicability of the method was verified by spiking four kinds of water and vegetable samples with PCAs, and satisfying recoveries were obtained (between 83.3% and 104.9%).
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http://dx.doi.org/10.1016/j.foodchem.2021.131090DOI Listing
September 2021

Uncovering the molecular mechanisms of Ilex pubescens against myocardial ischemia-reperfusion injury using network pharmacology analysis and experimental pharmacology.

J Ethnopharmacol 2022 Jan 17;282:114611. Epub 2021 Sep 17.

Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, China. Electronic address:

Ethnopharmacological Relevance: Ilex pubescens (I. pubescens), has been widely used to treat cardiovascular disease (CVD) in South China. Several studies have revealed aspect of its phytochemistry and pharmacological activities in cardiovascular diseases, but its active compounds and mechanisms of action are still unclear. The aim of this study was to search for the active compounds and the pharmacological mechanisms of I. pubescens for myocardial ischemia-reperfusion injury (MI/RI) by an integrative pharmacology-based investigation.

Materials And Methods: The main targets of compounds in I. pubescens were predicted using the TargetNet webserver (http://targetnet.scbdd.com). The network between compounds and predicted targets related to MI/RI and compounds was constructed. Functional enrichment analysis was performed to investigate the specific functions and pathways involved in the candidate I. pubescens targets acting on MI/RI, which were further validated by in vitro and in vivo experiments.

Results: A total of 191 targets were predicted for 64 chemical compounds in I. pubescens. Following Venn's analysis, we found that 38 candidate targets of I. pubescens were associated with protective effects against MI/RI. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these targets were related to estrogen signaling pathway. Importantly, the cardioprotective effects of I. pubescens and its active compounds were evaluated and the regulatory effects on key targets of heat shock protein 90 alpha family class A member 1 (HSP90AA1) and Estrogen receptor 1 (ESRα) in estrogen signaling pathway were validated in vitro and in vivo.

Conclusion: Our discoveries revealed that I. pubescens ameliorated MI/RI by regulating HSP90AA1 and ESRα in estrogen signaling pathway.
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http://dx.doi.org/10.1016/j.jep.2021.114611DOI Listing
January 2022

Selective Heavy Atom Effect Forming Photosensitizing Hot Spots in Double-Stranded DNA Matrix.

J Phys Chem Lett 2021 Sep 16;12(38):9205-9212. Epub 2021 Sep 16.

Analytical & Testing Centre, Sichuan University, Chengdu 610064, China.

Triplet exciton formation is essential for photosensitization-based photochemistry and photobiology. The heavy atom effect (HAE), in the form of either external or internal mode, is a basic mechanism for increasing the triplet exciton yield of photosensitizers. Herein, we report a new HAE mode by noncovalent cohosting of heavy atoms and photosensitizers in a double-stranded DNA (dsDNA) matrix. With dsDNA bearing several thymine (T) or cytosine (C) mismatches, heavy atoms (e.g., Hg or Ag) and dsDNA-staining dyes (photosensitizers) were spatially adjoined in close proximity, thus resulting in enhanced phosphorescence and O generation from the photosensitizers. The dsDNA-hosted HAE provides highly selective recognition for the heavy atoms, which is not applicable in either the external or the internal mode. Considering the simpleness and efficiency of the spatially adjoined HAE, as well as the functionality of DNA, the proposed HAE mode is appealing for various singlet oxygen- and phosphorescence-related applications.
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http://dx.doi.org/10.1021/acs.jpclett.1c02809DOI Listing
September 2021

Optimization of 4D flow MRI velocity field in the aorta with divergence-free smoothing.

Med Biol Eng Comput 2021 Sep 15. Epub 2021 Sep 15.

Department of Structural Heart Disease, Chinese Academy of Medical Sciences & Fuwai Hospital; State Key Laboratory of Cardiovascular Disease, Peking Union Medical College, 167 Beilishi Road, Xicheng District, 100037, Beijing, China.

Divergence-free smoothing with wall treatment (DFS) method is proposed for processing with four-dimensional (4D) flow magnetic resonance imaging (MRI) data of blood flows to enhance the quality of flow field with physical constraints. The new method satisfies the no-slip wall boundary condition and applies wall function of velocity profile for better estimating the velocity gradient in the near-wall region, and consequently improved wall shear stress (WSS) calculation against the issue of coarse resolution of 4D flow MRI. In the first testing case, blood flow field obtained from 4D flow MRI is well smoothed by DFS method. A great consistency is observed between the post-processed 4D flow MRI data and the computational fluid dynamics (CFD) data in the interested velocity field. WSS has an apparent improvement due to the proposed near-wall treatment with special wall function comparing to the result from original 4D flow MRI data or the DFS-processed data with no wall function. The other five cases also show the same performance that smoothed velocity field and improved WSS estimation are achieved on 4D flow MRI data optimized by DFS. The improvements will benefit the study of hemodynamics regarding the determination of location or the potential possibility of lesions.
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http://dx.doi.org/10.1007/s11517-021-02417-8DOI Listing
September 2021

A Fast-Responsive OFF-ON Near-Infrared-II Fluorescent Probe for In Vivo Detection of Hypochlorous Acid in Rheumatoid Arthritis.

Anal Chem 2021 09 15;93(38):13014-13021. Epub 2021 Sep 15.

Research Center for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China.

Rheumatoid arthritis (RA) is a common chronic autoimmune inflammatory disease, and its etiology is closely related to the overproduction of hypochlorous acid (HClO). However, early detection of RA using an activatable near-infrared-II (NIR-II, 1000-1700 nm) fluorescent probe remains challenging. Herein, we first report an "OFF-ON" NIR-II fluorescent probe named (phenothiazine triphenylamine) for imaging HClO in deep-seated early RA. Electron-rich phenothiazine in the core of was utilized as both an HClO-recognition moiety and a precursor of electron acceptors, displaying a typical donor-acceptor-donor structure with excellent NIR-II emission at 936/1237 nm once reacted with HClO. The probe exhibited good water solubility, high photostability, and rapid response capability toward HClO within 30 s. Moreover, it was able to sensitively and specifically detect exogenous and endogenous HClO in living cells in both visible and NIR-II windows. Notably, enabled the sensitive and rapid visualization of HClO generation in an inflammatory RA mouse model, showing a 4.3-fold higher NIR-II fluorescence intensity than that in normal hindlimb joints. These results demonstrate that holds great promise as a robust platform for diagnosis of HOCl-mediated inflammatory disorders.
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http://dx.doi.org/10.1021/acs.analchem.1c02831DOI Listing
September 2021

SO Poisoning Mechanism of the Multi-active Center Catalyst for Chlorobenzene and NO Synergistic Degradation at Dry and Humid Environments.

Environ Sci Technol 2021 10 15;55(19):13186-13197. Epub 2021 Sep 15.

School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, P. R. China.

The performance of fresh (PdV/TiO), sulfur poisoned (Used-S and Used-H), and regenerated (Used-R and Used-R) multi-active center catalysts for chlorobenzene catalytic oxidation and selective catalytic reduction (CBCO + SCR) reaction is investigated. The reaction on the catalyst surface is blocked after sulfur poisoning owing to the occupation and deposition of catalyst active centers (mainly Pd centers) by PdSO (and/or PdS in a dry environment) and NHHSO species, especially the CBCO process. Sulfates (mainly NHHSO) on the sulfur poisoned catalyst surface are partially decomposed after 400 °C thermal regeneration, while the deactivation caused by the formation of PdSO species is irreversible. Density functional theory calculation results show that in the PdSO and NHHSO generation paths, each step of the elementary reaction has just a small energy barrier to overcome, and the stability of the product for each elementary reaction increases gradually. Even worse, SO is easily combined with HO gas molecules to form HSO in a humid environment, and the energy barrier for conversion of SO to SO is just 0.041 eV. The two oxygen vacancies (VO or TiO) provide adsorption sites for CBCO + SCR reaction gas molecules but do not exhibit adsorption properties for SO, which gives a possible idea for optimization of sulfur resistance. The present work is favorable for further synergistic removal of CB/NO by the catalyst for anti-SO poisoning modification and application in the manufacture industry.
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http://dx.doi.org/10.1021/acs.est.1c03617DOI Listing
October 2021

A One-pot-synthesized Double-layered Anticoagulant Hydrogel Tube.

Chem Res Chin Univ 2021 Sep 4:1-7. Epub 2021 Sep 4.

GBA Research Innovation Institute for Nanotechnology, Guangzhou, 510700 P. R. China.

Extracorporeal membrane oxygenation(ECMO) has emerged as a viable treatment in severe cases of acute respiratory distress syndrome, acute respiratory failure, and adult respiratory distress syndrome. However, thromboembolic events stemming from the use of ECMO devices results in significant morbidity and mortality rates; the inner surface of the ECMO tubing comes into contact with the blood and can readily initiate coagulation. In addition, the tubing needs to be continually replaced due to thromboses on the inner tube wall, which not only increases the risk of infection but also the economic burden. Despite considerable effort, a surface modification strategy that effectively addresses these challenges has not yet been realized. In this study, we developed an integrated hollow core-shell-shell hydrogel tube of gelatin/alginate/acrylamide-bacterial nanocellulose(GAA) that meets the anticoagulant requirements for the inner tubing layer as well as the highly elastic soft material needed for the outer layer. Using static blood from healthy volunteers, we confirmed that the platelets or coagulation is not stimulated by the GAA tubing. Importantly, experiments with dynamic blood also demonstrated that the inner layer of the tubing does not elicit blood clotting. The one-pot-synthesized process may provide guidance for the design of anticoagulation tubes used clinically.

Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s40242-021-1267-3.
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http://dx.doi.org/10.1007/s40242-021-1267-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418287PMC
September 2021

RNA Methylations in Cardiovascular Diseases, Molecular Structure, Biological Functions and Regulatory Roles in Cardiovascular Diseases.

Front Pharmacol 2021 19;12:722728. Epub 2021 Aug 19.

Department of Anatomy, School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China.

Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality in the world. Despite considerable progress in the diagnosis, treatment and prognosis of CVDs, new diagnostic biomarkers and new therapeutic measures are urgently needed to reduce the mortality of CVDs and improve the therapeutic effect. RNA methylations regulate almost all aspects of RNA processing, such as RNA nuclear export, translation, splicing and non-coding RNA processing. In view of the importance of RNA methylations in the pathogenesis of diseases, this work reviews the molecular structures, biological functions of five kinds of RNA methylations (m6A, m5C, m1a, m6am and m7G) and their effects on CVDs, including pulmonary hypertension, hypertension, vascular calcification, cardiac hypertrophy, heart failure. In CVDs, m6A "writers" catalyze the installation of m6A on RNAs, while "erasers" remove these modifications. Finally, the "readers" of m6A further influence the mRNA splicing, nuclear export, translation and degradation. M5C, m1A, m6Am and m7G are new types of RNA methylations, their roles in CVDs need to be further explored. RNA methylations have become a new research hotspot and the roles in CVDs is gradually emerging, the review of the molecular characteristics, biological functions and effects of RNA methylation on CVDs will contribute to the elucidation of the pathological mechanisms of CVDs and the discovery of new diagnostic markers and therapeutic targets of CVDs.
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http://dx.doi.org/10.3389/fphar.2021.722728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417252PMC
August 2021

The association between physical activity, sedentary time and health-related quality of life in cancer survivors.

Health Qual Life Outcomes 2021 Sep 6;19(1):213. Epub 2021 Sep 6.

Institute of Clinical Epidemiology, Key Laboratory of Public Health Safety, Ministry of Education, Key Lab of Health Technology Assessment of Ministry of Health, School of Public Health, Fudan University, 130 Dong-An Road, Shanghai, 200032, China.

Background: Although physical activity (PA) and sedentary time in cancer survivors (CSs) were associated with health-related quality of life (HRQOL), it was not clear whether their associations were similar among CSs with different number of comorbid chronic diseases (CCDs). This study aimed to investigate the associations between PA, sedentary time and HRQOL in CSs with different number of CCDs.

Methods: A cross-sectional study was conducted among 1546 CSs between June and September 2018 in Shanghai, China. Data were collected with a self-reported questionnaire including sociodemographic characteristics, CCDs, PA, sedentary time and HRQOL. International Physical Activity Questionnaire and Cancer Quality of Life Questionnaire-Core30 were respectively used to measure PA and HRQOL of CSs. Associations of PA and sedentary time with HRQOL among CSs with different number of CCDs were evaluated by using logistic regression, adjusted for confounding factors.

Results: About seventy-five percent CSs had at least one CCD. Approximately three fifths CSs had high PA level and < 4 h/day sedentary time. Moderate PA level and high PA level were shown to be associated with better HRQOL among all participants. In CSs with ≤ 2 CCDs, high PA level was significantly associated with higher scores of physical function and lower scores of nausea and vomiting, appetite loss. However, there was a positive association between high PA level and constipation score among CSs with ≥ 3 CCDs. CSs with shorter sedentary time had better HRQOL in those with CCDs.

Conclusions: High PA level and long sedentary time have significant association with worse HRQOL of CSs with ≥ 3 CCDs, while high PA level is positively associated with HRQOL in CSs with ≤ 2 CCDs. Our findings may support further studies of the causal association between PA, sedentary times and HRQOL to provide targeted proposal to improve the HRQOL of CSs according to their number of CCDs.
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http://dx.doi.org/10.1186/s12955-020-01575-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419900PMC
September 2021

Large eddy simulation as a fast and accurate engineering approach for the simulation of rotary blood pumps.

Int J Artif Organs 2021 Sep 2:3913988211041636. Epub 2021 Sep 2.

School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing, China.

An accurate representation of the flow field in blood pumps is important for the design and optimization of blood pumps. The primary turbulence modeling methods applied to blood pumps have been the Reynolds-averaged Navier-Stokes (RANS) or URANS (unsteady RANS) method. Large eddy simulation (LES) method has been introduced to simulate blood pumps. Nonetheless, LES has not been widely used to assist in the design and optimization of blood pumps to date due to its formidable computational cost. The purpose of this study is to explore the potential of the LES technique as a fast and accurate engineering approach for the simulation of rotary blood pumps. The performance of "Light LES" (using the same time and spatial resolutions as the URANS) and LES in two rotary blood pumps was evaluated by comparing the results with the URANS and extensive experimental results. This study showed that the results of both "Light LES" and LES are superior to URANS, in terms of both performance curves and key flow features. URANS could not predict the flow separation and recirculation in diffusers for both pumps. In contrast, LES is superior to URANS in capturing these flows, performing well for both design and off-design conditions. The differences between the "Light LES" and LES results were relatively small. This study shows that with less computational cost than URANS, "Light LES" can be considered as a cost-effective engineering approach to assist in the design and optimization of rotary blood pumps.
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http://dx.doi.org/10.1177/03913988211041636DOI Listing
September 2021

Cobalt Carbonyls Stabilized by N,P-Ligands: Synthesis, Structure, and Catalytic Property for Ethylene Oxide Hydroalkoxycarbonylation.

Chem Asian J 2021 Sep 2. Epub 2021 Sep 2.

State Key Laboratory of Physical Chemistry of Solid Surfaces, National Engineering Laboratory for Green Chemical Productions of Alcohols-Ethers-Esters, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P. R. China.

Reactions of N,P-Ligands as Ph P(o-NMe C H ) ( L), 2,6-iPr C H NHC(Ph)=NC H (o-PPh ) ( L), and Ph PN(R)PPh (R=iPr ( L), cyclo-C H ( L), tBu ( L), CH C H O ( L)) each with dicobalt octacarbonyl produced complexes [ LCo(CO) ] (1), [ LCo(CO)(μ-CO) Co(CO) ] (2), [ LCo(CO) ] [Co(CO) ] (3), [ LCo(CO) ] (4), [ LCo(CO) ] (5), [ LCo(CO) ] [Co(CO) ] (6), and [ LCo(CO) ] [Co(CO) ] (7). Complexes 1-7 have all been structurally characterized by X-ray crystallography, IR and NMR spectroscopies, and elemental analysis. Catalytic tests on transformation of ethylene oxide (EO), CO and MeOH into methyl 3-hydroxypropionate (3-HMP) indicate that complexes 1-7 are active, where ion-pair complexes 3 and 6-7 behave more excellently (by achieving 88.4-93.6% 3-HMP yields) than the neutral species 1-2 and 4-5 (35.0-46.5% 3-HMP yields) when the reactions are all operated at 2 MPa CO pressure and 50 °C in MeOH solvent. Density functional theory (DFT) study by selecting 3 as a model suggests a cooperative catalytic reaction mechanism by [Co(CO) ] and its counter cation [ LCo(CO) ] . The cobalt-homonuclear ion-pair catalyzed hydroalkoxycarbonylation of EO is present herein.
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http://dx.doi.org/10.1002/asia.202100688DOI Listing
September 2021
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