Publications by authors named "Peng Wang"

5,757 Publications

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Silicon-Stereogenic Monohydrosilane: Synthesis and Applications.

Authors:
Yichen Wu Peng Wang

Angew Chem Int Ed Engl 2022 May 20. Epub 2022 May 20.

Shanghai Institute of Organic Chemistry, State key laboratory of organometallic chemistry, 345 Lingling Rd, 200032, Shanghai, CHINA.

Optical organosilanes have been demonstrated as versatile chiral reagents in synthetic chemistry since Sommer and Corriu's early seminal contributions. Among those charming silicon-containing chiral architectures, monohydrosilane, bearing a Si-H bond on the silicon center, hold its unique position due to its facile transformations via stereospecific Si-carbon or Si-heteroatom bond formation reactions. In addition, those compounds have also been leveraged as chiral reagents for alcohol resolution, chiral auxiliaries, mechanism probes, as well as potential optoelectronic materials. This minireview comprehensively summarizes the synthesis and synthetic applications of the silicon-stereogenic monohydrosilane, particularly the advances in transition metal-catalyzed asymmetric synthesis of this class of functional molecules.
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http://dx.doi.org/10.1002/anie.202205382DOI Listing
May 2022

Co-exposure of Monensin Increased the Risks of Atrazine to Earthworms.

Environ Sci Technol 2022 May 20. Epub 2022 May 20.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing 100193, P.R. China.

Antibiotics could enter farmlands through sewage irrigation or manure application, causing combined pollution with pesticides. Antibiotics may affect the environmental fate of pesticides and even increase their bioavailability. In this study, the influence of monensin on the degradation, toxicity, and availability of atrazine in soil-earthworm microcosms was investigated. Monensin inhibited the degradation of atrazine, changed the metabolite patterns in soil, and increased the bioavailability of atrazine in earthworms. Atrazine and monensin had a significant synergistic effect on earthworms in the acute toxic test. In long-term toxicity tests, co-exposure of atrazine and monensin also led to worse effects on earthworms including oxidative stress, energy metabolism disruption, and cocoon production compared to single exposure. The expression of tight junction proteins was down-regulated significantly by monensin, indicating that the intestinal barrier of earthworms was weakened, possibly causing the increased bioavailability of atrazine. The expressions of heat shock protein 70 (Hsp70) and reproductive and ontogenetic factors (ANN, TCTP) were all downregulated in binary exposure, indicating that the resilience and cocoon production of earthworms were further weakened under combined pollution. Monensin disturbed the energy metabolism and weakened the intestinal barrier of earthworms. These results showed that monensin increased the risks of atrazine in agricultural areas.
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http://dx.doi.org/10.1021/acs.est.2c00226DOI Listing
May 2022

Cotton miR393-TIR1 Module Regulates Plant Defense Against via Auxin Perception and Signaling.

Front Plant Sci 2022 3;13:888703. Epub 2022 May 3.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.

Plant auxin is essential in plant growth and development. However, the molecular mechanisms of auxin involvement in plant immunity are unclear. Here, we addressed the function of the cotton () miR393-TIR1 module in plant defense against infection via auxin perception and signaling. was directedly cleaved by ghr-miR393 according to mRNA degradome data, 5'-RACE analysis, and a GUS reporter assay. Ghr-miR393 knockdown significantly increased plant susceptibility to compared to the control, while ghr-miR393 overexpression and knockdown significantly increased plant resistance. External indole-3-acetic acid (IAA) application significantly enhanced susceptibility to in ghr-miR393 knockdown and control plants compared to mock treatment, and only slightly increased susceptibility in overexpressing ghr-miR393 and GhTIR1-silenced plants. Application of external PEO-IAA (an auxin antagonist) had a contrary trend with IAA application. Based on yeast two-hybrid and bimolecular fluorescence complementation assays, GhTIR1 interacted with GhIAA14 in the nucleus, and knockdown reduced plant resistance to infection. The results suggested that the ghr-miR393-GhTIR1 module regulates plant defense via auxin perception and signaling. Additionally, simultaneous knockdown of and significantly increased plant susceptibility to compared to the control, indicating that salicylic acid (SA) accumulation is vital for the ghr-miR393-GhTIR1 module to regulates plant resistance. Transcriptome data also demonstrated that knockdown significantly downregulated expression of auxin-related genes and upregulated expression of SA-related genes. Overall, the ghr-miR393-GhTIR1 module participates in plant response to infection via IAA perception and signaling partially depending on the SA defense pathway.
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http://dx.doi.org/10.3389/fpls.2022.888703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111529PMC
May 2022

Efficient Path Planning for Mobile Robot Based on Deep Deterministic Policy Gradient.

Sensors (Basel) 2022 May 8;22(9). Epub 2022 May 8.

Information Science and Electrical Engineering, Shandong Jiao Tong University, Jinan 250357, China.

When a traditional Deep Deterministic Policy Gradient (DDPG) algorithm is used in mobile robot path planning, due to the limited observable environment of mobile robots, the training efficiency of the path planning model is low, and the convergence speed is slow. In this paper, Long Short-Term Memory (LSTM) is introduced into the DDPG network, the former and current states of the mobile robot are combined to determine the actions of the robot, and a Batch Norm layer is added after each layer of the Actor network. At the same time, the reward function is optimized to guide the mobile robot to move faster towards the target point. In order to improve the learning efficiency, different normalization methods are used to normalize the distance and angle between the mobile robot and the target point, which are used as the input of the DDPG network model. When the model outputs the next action of the mobile robot, mixed noise composed of Gaussian noise and Ornstein-Uhlenbeck (OU) noise is added. Finally, the simulation environment built by a ROS system and a Gazebo platform is used for experiments. The results show that the proposed algorithm can accelerate the convergence speed of DDPG, improve the generalization ability of the path planning model and improve the efficiency and success rate of mobile robot path planning.
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http://dx.doi.org/10.3390/s22093579DOI Listing
May 2022

A Novel Central Camera Calibration Method Recording Point-to-Point Distortion for Vision-Based Human Activity Recognition.

Sensors (Basel) 2022 May 5;22(9). Epub 2022 May 5.

Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China.

The camera is the main sensor of vison-based human activity recognition, and its high-precision calibration of distortion is an important prerequisite of the task. Current studies have shown that multi-parameter model methods achieve higher accuracy than traditional methods in the process of camera calibration. However, these methods need hundreds or even thousands of images to optimize the camera model, which limits their practical use. Here, we propose a novel point-to-point camera distortion calibration method that requires only dozens of images to get a dense distortion rectification map. We have designed an objective function based on deformation between the original images and the projection of reference images, which can eliminate the effect of distortion when optimizing camera parameters. Dense features between the original images and the projection of the reference images are calculated by digital image correlation (DIC). Experiments indicate that our method obtains a comparable result with the multi-parameter model method using a large number of pictures, and contributes a 28.5% improvement to the reprojection error over the polynomial distortion model.
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http://dx.doi.org/10.3390/s22093524DOI Listing
May 2022

A novel peptide-based fluorescent probe for highly selective detection of mercury (II) ions in real water samples and living cells based on aggregation-induced emission effect.

Anal Bioanal Chem 2022 May 20. Epub 2022 May 20.

Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, People's Republic of China.

A new fluorescent probe TPE-GHK was synthesized containing a tetrastyrene (TPE) derivative as fluorophore and classical tripeptide (Gly-His-Lys-NH) as a receptor based on the aggregation-induced emission (AIE) mechanism. TPE-GHK displayed high selectivity and rapid fluorescent "turn-on" response to Hg among other competitive metal ions. The 2:1 complex binding mechanism of TPE-GHK toward Hg was verified by fluorometric titration, Job's plots, and ESI-HRMS spectra. The fluorescent emission showed a good linear response in the range of 0-1.0 μM with the low detection limit of 28.6 nM. Meanwhile, TPE-GHK exhibited the excellent biocompatibility and low toxicity and was successfully applied in monitoring Hg in living CAKI 2 cells, which demonstrated its potential application in environment and biological science. More importantly, TPE-GHK could be used to detect Hg in two real water samples and also was successfully designed as test strips.
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http://dx.doi.org/10.1007/s00216-022-04094-4DOI Listing
May 2022

Short-term exposure to nitrogen dioxide and ischemic stroke incidence in Shenzhen, China: Modification effects by season and temperature.

Ecotoxicol Environ Saf 2022 May 16;239:113644. Epub 2022 May 16.

Department of Environment and Health, Shenzhen Center for Disease Control and Prevention, 8 Longyuan Rd, Shenzhen 518055, Guangdong, China. Electronic address:

Background And Objectives: China has experienced a serious public health burden because of the increased incidence of ischemic stroke. Evidence describing the association between short-term exposure to nitrogen dioxide (NO) and ischemic stroke morbidity is limited, and few studies have focused on the effects of season and temperature. This study aimed to evaluate the acute effects of NO on ischemic stroke incidence in Shenzhen, a southeastern city of China, considering the modified effects of season and temperature.

Methods: A time-stratified case-crossover study was conducted between 2003 and 2014 among 98,482 ischemic stroke hospitalizations. Conditional quasi-Poisson regression was used to estimate the percentage changes in ischemic stroke admissions in relation to each 10 μg/m increment in NO.

Results: NO was positively associated with ischemic stroke onset over the full year, as well as in the cold season (November through April) and on cold days (ambient temperature≤median temperature), with significant single-day effects within 3 days after the exposure, and significant cumulative effects within the delayed five days. The maximum percentage changes were obtained at lag0-5, with 1.81% (95% confidence interval (CI) was 0.86-2.76%) over the full year, 2.75% (1.48-4.03%) in the cold season, and 3.04% (1.74-4.35%) on cold days. Additionally, the effects of exposure were found to be greater in males and people with higher education, and were lasting longer in subgroups of older individuals.

Conclusions: Our findings provide evidence that reductions in NO levels might decrease ischemic stroke morbidity, and enhance the understanding of ischemic stroke occurrence associated with NO modified by season and temperature.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113644DOI Listing
May 2022

Protective effect of homogeneous polysaccharides of Wuguchong (HPW) on intestinal mucositis induced by 5-fluorouracil in mice.

Nutr Metab (Lond) 2022 May 18;19(1):36. Epub 2022 May 18.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, Dalian, China.

Background: In hospitalized patients, drug side effects usually trigger intestinal mucositis (IM), which in turn damages intestinal absorption and reduces the efficacy of treatment. It has been discovered that natural polysaccharides can relieve IM. In this study, we extracted and purified homogenous polysaccharides of Wuguchong (HPW), a traditional Chinese medicine, and explored the protective effect of HPW on 5-fluorouracil (5-FU)-induced IM.

Methods And Results: First, we identified the physical and chemical properties of the extracted homogeneous polysaccharides. The molecular weight of HPW was 616 kDa, and it was composed of 14 monosaccharides. Then, a model of small IM induced by 5-FU (50 mg/kg) was established in mice to explore the effect and mechanism of HPW. The results showed that HPW effectively increased histological indicators such as villus height, crypt depth and goblet cell count. Moreover, HPW relieved intestinal barrier indicators such as D-Lac and diamine oxidase (DAO). Subsequently, western blotting was used to measure the expression of Claudin-1, Occludin, proliferating cell nuclear antigen, and inflammatory proteins such as NF-κB (P65), tumour necrosis factor-α (TNF-α), and COX-2. The results also indicated that HPW could reduce inflammation and protect the barrier at the molecular level. Finally, we investigated the influence of HPW on the levels of short-chain fatty acids, a metabolite of intestinal flora, in the faeces of mice.

Conclusions: HPW, which is a bioactive polysaccharide derived from insects, has protective effects on the intestinal mucosa, can relieve intestinal inflammation caused by drug side effects, and deserves further development and research.
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http://dx.doi.org/10.1186/s12986-022-00669-1DOI Listing
May 2022

A Biodegradable High-Efficiency Magnetic Nanoliposome Promotes Tumor Microenvironment-Responsive Multimodal Tumor Therapy Along with Switchable T Magnetic Resonance Imaging.

ACS Appl Mater Interfaces 2022 May 18. Epub 2022 May 18.

School of Pharmacy, the Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, P. R. China.

We explored the catalytic activity and magnetic resonance imaging (MRI) capacity of Cu-doped ultrasmall iron oxides with different doping ratios. Then, we screened a highly efficient ultrasmall active catalyst (UAC). Subsequently, a biodegradable magnetic nanoliposome was developed for multimodal cancer theranostics through pH-sensitive liposome coating of these UACs. Upon entering the body, the magnetic nanoliposomes significantly prolonged the metabolic time of UACs and promoted their accumulation in tumors. Then, the strong photothermal (PT) effect of the magnetic nanoliposome quickly ablated the tumor, showing promising PT therapy. Upon entering tumor cells, the magnetic nanoliposome rapidly degraded into many UACs and released chemotherapeutic drugs, contributing to chemotherapy. In addition, UACs not only catalyzed Fenton-type reaction to produce excessive reactive oxygen species (ROS) but also inhibited the synthesis of endogenous GSH by inactivating glutamyl cysteine ligase, contributing to cancer ferroptosis. Furthermore, the assembly-dissociation process of UACs showed the function of magnetic relaxation switches, significantly enhancing tumor MRI signal change, achieving a more accurate diagnosis of the tumor. Therefore, this magnetic nanoliposome splits into many UACs upon drug release and regulates the tumor microenvironment to overproduce ROS for enhanced synergistic tumor theranostics, which provides a strategy for developing next-generation magnetic catalysts with biodegradability and multimodal antitumor theranostics.
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http://dx.doi.org/10.1021/acsami.2c04158DOI Listing
May 2022

Benzodiazepine Derivatives as Potent Vasopressin V Receptor Antagonists for the Treatment of Autosomal Dominant Kidney Disease.

J Med Chem 2022 May 17. Epub 2022 May 17.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou 221004, Jiangsu, China.

Cyst formation and enlargement in autosomal dominant kidney disease (ADPKD) is mainly driven by aberrantly increased cytosolic cAMP in renal tubule epithelial cells. Because the vasopressin V receptor (VR) regulates intracellular cAMP levels in kidneys, a series of benzodiazepine derivatives were developed targeting the VR. Among these derivatives, compound exhibited potent binding affinity to the VR ( = 9.0 ± 1.5 nM) and efficacious cAMP inhibition (IC = 9.2 ± 3.0 nM). This led to the suppression of cyst formation and growth in both an MDCK cell model and an embryonic kidney cyst model. Further advancing compound in a murine model of ADPKD demonstrated a significantly improved efficacy compared with the reference compound tolvaptan. Overall, compound holds therapeutic potential for the treatment of ADPKD.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00567DOI Listing
May 2022

NEDD9 Mediates the FAK/Src Signaling Pathway to Promote the Adhesion of Human Trabecular Meshwork Cells after Dexamethasone Treatment.

Curr Eye Res 2022 May 16:1-9. Epub 2022 May 16.

The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab of Ophthalmology, Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, P. R. China.

Purpose: The differential gene expression of neural precursor cell expressed developmentally downregulated 9 (NEDD9) in human trabecular meshwork (HTM) cells after dexamethasone (Dex) treatment was confirmed through gene expression profiling. However, the regulatory mechanism of NEDD9 expression in HTM cells remains unknown. In this study, we investigated NEDD9 expression in HTM cells and gained a better understanding of glucocorticoid-induced glaucoma (GIG) pathophysiology.

Methods: The Gene Expression Omnibus database and GEO2R tool were used to identify differentially expressed genes in the GSE37474 and GSE124114 datasets, and NEDD9 gene expression was found to be upregulated. Human corneoscleral segments and HTM cells were treated with 100 nM Dex or an equal volume of ethanol (0.01%) for 7 days. NEDD9 expression in TM tissues was evaluated by immunohistochemistry, and NEED9 expression in HTM cells was confirmed by RT-qPCR and western blotting. HTM cell adhesive behaviors were assessed with a cell adhesion detection kit. NEDD9 expression was knocked down with short hairpin RNA in HTM cells, and FAK/Src signaling pathway activation was found to be regulated by NEDD9.

Results: After 7 days of HTM cell Dex treatment, NEDD9 expression was upregulated to be approximately twice that of control. FAK, Src, phospho-FAK, and phospho-Src expression in Dex-treated HTM cells was markedly increased. Downregulation of NEDD9 expression reduced HTM cell adhesion to the surface of culture wells and simultaneously led to a reduction in FAK, Src, phospho-FAK and phospho-Src expression.

Conclusions: NEDD9 expression is upregulated in HTM cells after Dex treatment and promotes HTM cell adhesion. These findings underscore the contribution of NEDD9 overexpression to altered HTM cell adhesion during glucocorticoid therapy and may play a key role in GIG pathological progression. Considering the similarity between GIG and primary open-angle glaucoma (POAG), our findings suggest that targeting NEDD9 may be a new therapeutic strategy for POAG patients.
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http://dx.doi.org/10.1080/02713683.2022.2071945DOI Listing
May 2022

The state of the art review on photocatalytic Cr(VI) reduction over MOFs-based photocatalysts: From batch experiment to continuous operation.

Chemosphere 2022 May 13;303(Pt 1):134949. Epub 2022 May 13.

School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, UK.

This state of the art review presented the photocatalytic reduction from highly toxic Cr(VI) to lowly toxic Cr(III) with metal-organic frameworks (MOFs) and their composites. The construction of composites facilitated the transportation of the photo-induced charges to enhance the Cr(VI) reduction, in which the corresponding mechanisms were clarified by both experimental tests and DFT calculations. The immobilized MOFs onto some substrates accomplished continuous operations toward Cr(VI) reduction even under real solar light. As well, the environmental applications of the Cr(VI) reduction were analyzed, in which the influence factors toward the Cr(VI) reduction were clarified. This review reported that a big breakthrough was achieved from the batch experiment to the continuous operation for Cr(VI) reduction, in which MOFs demonstrated a bright prospective in the field of photocatalytic Cr(VI) reduction.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134949DOI Listing
May 2022

Pivotal roles of nanoscale zerovalent iron supported on metal-organic framework material in cadmium (II) migration and transformation in soil.

J Environ Sci Health B 2022 May 15:1-11. Epub 2022 May 15.

Tianjin Key Laboratory of Water Resources and Environment, Tianjin Normal University, Tianjin, China.

Cadmium (Cd) contamination in soils is of great concern, and therefore the development of effective remediation technologies for cadmium contamination is urgent. In our study, nano zero-valent iron (NZVI) supported by metal-organic framework (MOF) materials (MOF-NZVI) were prepared using NaBH and FeCl to try to solve the soil Cd remediation problem. Herein, the effects and the mechanism of MOF-NZVI for the immobilization of Cd in contaminated soil was investigated. The results showed that MOF-NZVI was capable of converting Cd in soil from weak acid extractable and reducible fractions to oxidizable and residual states, thus effectively reducing the toxicity of Cd in soil. FTIR and XRD analysis confirmed that the dominant reaction mechanism between MOF-NVZI and Cd is adsorption with complexation, and the stabilization of Cd is achieved by the formation of compounds such as FeOCdOH.
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http://dx.doi.org/10.1080/03601234.2022.2071561DOI Listing
May 2022

A Survey of Multifingered Robotic Manipulation: Biological Results, Structural Evolvements, and Learning Methods.

Front Neurorobot 2022 27;16:843267. Epub 2022 Apr 27.

State Key Laboratory for Management and Control of Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China.

Multifingered robotic hands (usually referred to as dexterous hands) are designed to achieve human-level or human-like manipulations for robots or as prostheses for the disabled. The research dates back 30 years ago, yet, there remain great challenges to effectively design and control them due to their high dimensionality of configuration, frequently switched interaction modes, and various task generalization requirements. This article aims to give a brief overview of multifingered robotic manipulation from three aspects: a) the biological results, b) the structural evolvements, and c) the learning methods, and discuss potential future directions. First, we investigate the structure and principle of hand-centered visual sensing, tactile sensing, and motor control and related behavioral results. Then, we review several typical multifingered dexterous hands from task scenarios, actuation mechanisms, and in-hand sensors points. Third, we report the recent progress of various learning-based multifingered manipulation methods, including but not limited to reinforcement learning, imitation learning, and other sub-class methods. The article concludes with open issues and our thoughts on future directions.
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http://dx.doi.org/10.3389/fnbot.2022.843267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097019PMC
April 2022

Osteogenesis of Iron Oxide Nanoparticles-Labeled Human Precartilaginous Stem Cells in Interpenetrating Network Printable Hydrogel.

Front Bioeng Biotechnol 2022 29;10:872149. Epub 2022 Apr 29.

School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing, China.

Smart biomaterials combined with stem cell-based therapeutic strategies have brought innovation in the field of bone tissue regeneration. However, little is known about precartilaginous stem cells (PCSCs), which can be used as seed cells and incorporated with bioactive scaffolds for reconstructive tissue therapy of bone defects. Herein, iron oxide nanoparticles (IONPs) were employed to modulate the fate of PCSCs, resulting in the enhanced osteogenic differentiation potential both and . PCSCs were isolated from the ring of La-Croix extracted from polydactylism patient and identified through immunohistochemically staining using anti-FGFR-3 antibodies. Potential toxicity of IONPs toward PCSCs was assessed through cell viability, proliferation, and attachment assay, and the results demonstrated that IONPs exhibited excellent biocompatibility. After that, the effects of IONPs on osteogenic differentiation of PCSCs were evaluated and enhanced ALP activity, formation of mineralized nodule, and osteogenic-related genes expressions could be observed upon IONPs treatment. Moreover, bone regeneration assessment was performed using rabbit femur defects as a model. A novel methacrylated alginate and 4-arm poly (ethylene glycol)-acrylate (4A-PEGAcr)-based interpenetrating polymeric printable network (IPN) hydrogel was prepared for incorporation of IONPs-labeled PCSCs, where 4A-PEGAcr was the common component for three-dimensional (3D) printing. The implantation of IONPs-labeled PCSCs significantly accelerated the bone formation process, indicating that IONPs-labeled PCSCs could endow current scaffolds with excellent osteogenic ability. Together with the fact that the IONPs-labeled PCSCs-incorporated IPN hydrogel (PCSCs-hydrogels) was biosafety and printable, we believed that PCSCs-hydrogels with enhanced osteogenic bioactivity could enrich the stem cell-based therapeutic strategies for bone tissue regeneration.
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http://dx.doi.org/10.3389/fbioe.2022.872149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099245PMC
April 2022

TLR2/TLR4-Enhanced TIPE2 Expression Is Involved in Post-Hemorrhagic Shock Mesenteric Lymph-Induced Activation of CD4+T Cells.

Front Immunol 2022 29;13:838618. Epub 2022 Apr 29.

Institute of Microcirculation, Hebei North University, Zhangjiakou, China.

Purpose: Post hemorrhagic shock mesenteric lymph (PHSML) return contributes to CD4 T cell dysfunction, which leads to immune dysfunction and uncontrolled inflammatory response. Tumor necrosis factor α induced protein 8 like-2 (TIPE2) is one of the essential proteins to maintain the immune homeostasis. This study investigated the role of TIPE2 in regulation of CD4 T lymphocyte function in interaction of PHSML and TLR2/TLR4.

Methods: The splenic CD4 T cells were isolated from various mice (WT, TLR2, TLR4) by immunomagnetic beads, and stimulated with PHSML, normal lymphatic fluid (NML), respectively. Application of TIPE2-carrying interfering fragments of lentivirus were transfected to WT, TLR4, and TLR2 CD4 T cells, respectively. After interference of TIPE2, they were stimulated with PHSML and NML for the examinations of TIPE2, TLR2, and TLR4 mRNA expressions, proliferation, activation molecules on surface, and cytokine secretion function.

Results: PHSML stimulation significantly upregulated TIPE2, TLR2, and TLR4 mRNA expressions, decreased proliferation, CD25 expression, and IFN-γ secretion, and increased the secretion ability of IL-4 in WT CD4 T cells. TIPE2 silencing enhanced proliferative capacity, upregulated CD25 expression, and increased IFNγ secretion in CD4 T cells. PHSML stimulated TLR2CD4 T or TLR4CD4 T cells of which TIPE2 were silenced. TLR2 or TLR4 knockout attenuated PHSML-induced CD4 T cells dysfunction; PHSML stimulation of silent TIPE2-expressing TLR2CD4 T or TLR4CD4 T revealed that the coexistence of low TIPE2 expression with lack of TLR2 or TLR4 eliminated this beneficial effect.

Conclusion: TIPE2 improves the PHSML-mediated CD4T cells dysfunction by regulating TLR2/TLR4 pathway, providing a new intervention target following hemorrhagic shock-induced immune dysfunction.
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http://dx.doi.org/10.3389/fimmu.2022.838618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101470PMC
May 2022

Use of sponge iron dosing in baffled subsurface-flow constructed wetlands for treatment of wastewater treatment plant effluents during autumn and winter.

Int J Phytoremediation 2022 May 15:1-13. Epub 2022 May 15.

School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, China.

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the effect of baffled subsurface-flow constructed wetland (BSFCW) with SI dosing to purify the WWTP effluents after the stable operation. The water flow of this BSFCW is the repeated combination of upward flow and downward flow, which can provide a longer treatment pathway and microbial exposure time. The average removal rates of total inorganic nitrogen (TIN) were 27.80%, 30.17%, and 44.83%, and the average removal rates of chemical oxygen demand (COD) were 19.96%, 23.73%, and 18.38%. The average removal rates of total phosphorus (TP) were 85.94%, 82.14%, and 83.95%. Cu/SI improved the dissolution of iron, C/SI improved denitrification, and a winter indoor temperature retention measure was adopted to increase the effectiveness of wetland treatment during the winter months. After comprehensively analyzing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and two-dimensional numerical simulation diagrams, a plausible conjecture that microbes use electrons from SI for autotrophic denitrification is presented. Moreover, the stress effect of wetlands dosed with SI on plants decreased stepwise along the course since C/SI used on wetlands had less impact on plant stress.
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http://dx.doi.org/10.1080/15226514.2022.2031866DOI Listing
May 2022

Genomic analysis of Thalassospira sp. SW-3-3 reveals its genetic potential for phthalate pollution remediation.

Mar Genomics 2022 Jun 25;63:100953. Epub 2022 Apr 25.

College of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China. Electronic address:

Thalassospira sp. SW-3-3 is a bacterial strain isolated from deep seawater of the Pacific Ocean at a water depth of 3112 m. It is a Gram-negative, aerobic, and curved rod-shaped bacterium belonging to the family Thalassospiraceae. In this study, we report the complete genome sequence of strain SW-3-3. It has a circular chromosome with a size of 4,764,478 bp and a G + C content of 54.7%. The genome contains 4296 protein-coding genes, 63 tRNA genes, and 12 rRNA genes. Genomic analysis shows that strain SW-3-3 contains genes and catalytic pathways relevant to phthalate metabolism. Phthalates are well-known emerging contaminants that are harmful to environments and human health. They are chemically stable compounds that are widely used in plastic products and are pervasive in our life. With the discharge of plastic pollutants, a huge number of phthalate compounds enter the ocean. The genetic information of strain SW-3-3 suggests that it has the potential to metabolize phthalates. There are 9 key enzymes in the metabolization pathway, and phthalates are finally catalyzed to produce succinyl-CoA which is further degraded through the tricarboxylic acid (TCA) cycle pathway. This genomic analysis will be helpful for further understanding of the applications of strain SW-3-3 in the remediation of phthalate pollution.
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http://dx.doi.org/10.1016/j.margen.2022.100953DOI Listing
June 2022

Free-Standing Nanoarrays with Energetic Electrons and Active Sites for Efficient Plasmon-Driven Ammonia Synthesis.

Small 2022 May 13:e2201269. Epub 2022 May 13.

State Key Laboratory of Crystal Materials, Institute of Crystal Materials, Shandong University, Jinan, 250100, China.

Direct ammonia (NH ) synthesis from water and atmospheric nitrogen using sunlight provides an energy-sustainable and carbon-neutral alternative to the Haber-Bosch process. However, the development of such a route with high performance is impeded by the lack of effective charge transfer and abundant active sites to initiate the nitrogen reduction reaction (NRR). Here, the authors report efficient plasmon-induced photoelectrochemical (PEC) NH synthesis on the hierarchical free-standing Au/K MoO /Mo/K MoO /Au nanoarrays. Endowed with energetically hot electrons and catalytically active sites, the plasmonic nanoarrays exhibit an efficient PEC NH synthesis rate of 9.6 µg cm h under visible light irradiation, which is among the highest PEC NRR systems. This work demonstrates the rationally designed plasmonic nanoarrays for highly efficient NH synthesis, which paves a new path for PEC catalytic reactions driven by surface plasmons and future monolithic PEC devices for direct artificial photosynthesis.
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http://dx.doi.org/10.1002/smll.202201269DOI Listing
May 2022

Perception of National Park Soundscape and Its Effects on Visual Aesthetics.

Int J Environ Res Public Health 2022 May 8;19(9). Epub 2022 May 8.

Research Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing 100091, China.

Soundscape perception is a very weak link in the national park landscape evaluation system in China. A thorough understanding of soundscapes and their effects on visual aesthetics is important for the management of national park landscapes. In this study, features of soundscapes (e.g., loudness, frequency, preference, and auditory satisfaction) were investigated based on 394 valid questionnaires of residents in the Qianjiangyuan National Park Pilot Area. The effects of soundscape on visual aesthetics were analyzed using the PLS-SEM. The results demonstrated that: (1) Peddling voice and insect sound were the loudest components in the soundscape, running water and birdsong were the most commonly heard and most preferred, religious sound was the quietest and least frequently heard, and horn was the least preferred. Residents in the Pilot Area were generally satisfied with the auditory environment. (2) Both sound frequency and preference have significant effects on auditory satisfaction, but preference (path coefficient = 0.426) has a larger effect than does frequency (path coefficient = 0.228). (3) Loudness has negligible effects on visual aesthetics, but other soundscape characteristics did influence visual aesthetics. Soundscape preference had the most significant effect (path coefficient = 0.305), followed by auditory satisfaction (path coefficient = 0.174), and sound frequency (path coefficient = 0.165). Among them, effects of perception frequency are the indirect utilities.
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http://dx.doi.org/10.3390/ijerph19095721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105284PMC
May 2022

Relative Contribution of Metal Content and Soil Particle Mass to Health Risk of Chromium-Contaminated Soil.

Int J Environ Res Public Health 2022 Apr 26;19(9). Epub 2022 Apr 26.

Guangdong Industrial Contaminated Site Remediation Technology and Equipment Engineering Research Center, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China.

Three soil samples from a chromium (Cr)-contaminated field were classified into five particle fractions (i.e., 0-50 μm, 50-100 μm, 100-250 μm, 250-500 μm, and 500-1000 μm) and were further characterized to study their physicochemical properties and Cr bioaccessibility. The results indicated that the gastrointestinal bioaccessibility estimated by the Solubility Bioaccessibility Research Consortium (SBRC) method was on average 15.9% higher than that by the physiologically based extraction test (PBET) method. The health risk of all samples was within the safe range, and the health risk based on total Cr content may be overestimated by an average of 13.2 times compared to the bioaccessibility-based health risk. The health risk investigated from metal content was mainly contributed by the 50-250 μm fraction, which was 47.5, 50.2, and 43.5% for low-, medium-, and high-level polluted soils, respectively. As for the combined effect, the fractions of 100-250 μm and 500-1000 μm contributed the highest proportion to health risk, which was 57.1, 62.1, and 64.4% for low-level, medium-level, and high-level polluted soils, respectively. These results may further deepen the understanding of health risk assessment and quantify the contribution of the soil particle mass to health risk.
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http://dx.doi.org/10.3390/ijerph19095253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100977PMC
April 2022

Effects of Tea Polyphenol Palmitate Existing in the Oil Phase on the Stability of Myofibrillar Protein O/W Emulsion.

Foods 2022 May 2;11(9). Epub 2022 May 2.

Key Laboratory of Meat Processing and Quality Control, MOE, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

This study aimed to explore the effect of adding different concentrations (0, 0.01%, 0.03%, and 0.05% (/)) of tea polyphenol palmitate (TPP) in the oil phase on the emulsifying properties of 5 and 10 mg/mL myofibrillar protein (MP). Particle size results revealed that the flocculation of droplets increased as TPP concentration increased and that droplets in 5 mg/mL MP emulsions (25-34 μm) were larger than in 10 mg/mL MP emulsions (14-22 μm). The emulsifying activity index of 5 mg/mL MP emulsions decreased with increasing TPP concentration. The micrographs showed that the droplets of MP emulsions exhibited extensive flocculation at TPP concentrations >0.03%. Compared with 5 mg/mL MP emulsions, 10 mg/mL MP emulsions showed better physical stability and reduced flocculation degree, which coincided with lower delta backscattering intensity (ΔBS) and Turbiscan stability index values. The flow properties of emulsions can be successfully depicted by Ostwald-de Waele models (R > 0.99). The concentrations of TPP and protein affect the K values of emulsions ( < 0.05). Altogether, increased protein concentration in the continuous phase could improve emulsion stability by increasing viscosity, offsetting the adverse effects of TPP to a certain extent. This study is expected to promote the rational application of TPP in protein emulsion products of high quality and acceptability.
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http://dx.doi.org/10.3390/foods11091326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104160PMC
May 2022

Du13 encodes a C H zinc-finger protein that regulates Wx pre-mRNA splicing and microRNA biogenesis in rice endosperm.

Plant Biotechnol J 2022 May 13. Epub 2022 May 13.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.

Amylose content is a crucial physicochemical property responsible for the eating and cooking quality of rice (Oryza sativa L.) grain and is mainly controlled by the Waxy (Wx) gene. Previous studies have identified several Dull genes that modulate the expression of the Wx allele in japonica rice by affecting the splicing efficiency of the Wx pre-mRNA. Here, we uncover dual roles for a novel Dull gene in pre-mRNA splicing and microRNA processing. We isolated the dull mutant, du13, with a dull endosperm and low amylose content. Map-based cloning showed that Du13 encodes a C H zinc-finger protein. Du13 coordinates with the nuclear cap-binding complex to regulate the splicing of Wx transcripts in rice endosperm. Moreover, Du13 also regulates alternative splicing of other protein-coding transcripts and affects the biogenesis of a subset of microRNAs. Our results reveal an evolutionarily conserved link between pre-mRNA splicing and microRNA biogenesis in rice endosperm. Our findings also provide new insights into the functions of Dull genes in rice and expand our knowledge of microRNA biogenesis in monocots.
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http://dx.doi.org/10.1111/pbi.13821DOI Listing
May 2022

Anti-POSTN and Anti-TIMP1 Autoantibodies as Diagnostic Markers in Esophageal Squamous Cell Carcinoma.

Front Genet 2022 26;13:860611. Epub 2022 Apr 26.

Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.

Esophageal cancer is one of the most commonly diagnosed malignant gastrointestinal tumors. The aim of the study was to explore the diagnostic values of anti-POSTN and anti-TIMP1 autoantibodies in esophageal squamous cell carcinoma (ESCC). Differentially expressed genes (DEGs) associated with esophageal cancer were screened out by the LIMMA method in the Gene Expression Profiling Interactive Analysis (GEPIA) platform. Search Tool for the Retrieval of Interacting Genes (STRING) was used to construct the protein-protein interaction (PPI) based on highly DEGs. The candidate hub genes were the intersection genes calculated based on degree and Maximal Clique Centrality (MCC) algorithms Cytoscape. A total of 370 participants including 185 ESCC patients and 185 matched normal controls were enrolled in enzyme-linked immunosorbent assay (ELISA) to detect the expression levels of autoantibodies corresponding to POSTN and TIMP1 proteins. A total of 375 DEGs with high expression were obtained in esophageal cancer. A total of 20 hub genes were acquired using the cytoHubba plugin by degree and MCC algorithms. The expression levels of anti-POSTN and anti-TIMP1 autoantibodies were higher in the sera of ESCC patients ( < 0.05). Anti-POSTN autoantibody can diagnose ESCC patients with an AUC of 0.638 at the specificity of 90.27% and sensitivity of 27.57%, and anti-TIMP1 autoantibody can diagnose ESCC patients with an AUC of 0.585 at the specificity of 90.27% and sensitivity of 20.54% ( < 0.05). In addition, anti-POSTN and anti-TIMP1 autoantibodies can distinguish ESCC patients from normal controls in most clinical subgroups ( < 0.05). In conclusion, anti-POSTN and anti-TIMP1 autoantibodies may be considered the potential biomarkers in the clinical diagnosis of ESCC.
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http://dx.doi.org/10.3389/fgene.2022.860611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087588PMC
April 2022

Natural variation in root exudation of GABA and DIMBOA impacts the maize root endosphere and rhizosphere microbiomes.

J Exp Bot 2022 May 11. Epub 2022 May 11.

Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA and Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.

Root exudates are important for shaping root-associated microbiomes. However, studies on a wider range of metabolites in exudates are required for a comprehensive understanding about their influence on microbial communities. We identified maize inbred lines that differ in exudate concentrations of DIMBOA and GABA using a semi-hydroponic system. These lines were grown in the field to determine the changes in microbial diversity and gene expression due to varying concentrations of DIMBOA and GABA in exudates using 16S rRNA amplicon sequencing and metatranscriptomics. Results showed individual and interaction effects of DIMBOA and GABA on the rhizosphere and root endosphere β-diversity, most strongly at the V10 growth stage. The main bacterial families affected by both compounds were Ktedonobacteraceae and Xanthomonadaceae. Higher concentrations of DIMBOA in exudates affected the rhizosphere metatranscriptome, enriching for KEGG pathways associated with plant disease. This study validated the use of natural variation within plant species as a powerful approach for understanding the role of root exudates on microbiome selection. We also showed that a semi-hydroponic system can be used to identify maize genotypes that differ in GABA and DIMBOA exudate concentrations under field conditions. The impact of GABA exudation on root-associated microbiomes was shown for the first time.
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http://dx.doi.org/10.1093/jxb/erac202DOI Listing
May 2022

sp. nov., isolated from a tidal flat sediment of the East China Sea.

Int J Syst Evol Microbiol 2022 May;72(5)

State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, PR China.

A Gram-stain-negative bacterial strain, designated as E165, was isolated from a tidal flat sediment of the East China Sea. Strain E165 grew optimally at pH 6, at 32 °C and with 1-2 % (w/v) NaCl. The 16S rRNA gene sequence similarity results revealed that strain E165 was most closely related to IVIA-Po-145, CK13, IVIA-Po-155, HDW-15, 46-16, B20-1 and DSL-35 with values of 97.0-98.5 %. The digital DNA-DNA hybridization and average nucleotide identity values between strain E165 and the reference strains were 21.9-34.3 % and 77.6-87.3 %, respectively. The DNA G+C content of the isolate was 42.9 mol%. Strain E165 contained Q-8 as the sole ubiquinone and C, summed feature 8 (C 7 and/or C 6) and summed feature 3 (C 7 and/or C 6) as the major fatty acids. The major polar lipids of strain E165 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and aminophospholipid. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, sp. nov., is proposed with E165 (=MCCC 1K06241=KCTC 82809) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.005368DOI Listing
May 2022

Procrastination and physical activity: The moderated mediating effect of grit.

J Am Coll Health 2022 May 12:1-9. Epub 2022 May 12.

Physical Education Department, Shanghai Jiao Tong University, Shanghai, China.

: To explore the relation between procrastination and physical activity in college students and test whether grit mediated, age and gender moderated the process. Participants: 610 college students (aged 20.07 ± 1.53; 45.2% males) in Shanghai. Procrastination, grit and physical activity were tested respectively by International Procrastination Scale, Grit-S scale and International Physical Activity Questionnaire. Correlation analysis showed that procrastination, grit and physical activity were related in pairs. Procrastination was negatively correlated with physical activity. Grit mediated between procrastination and physical activity. Females showed stronger moderation effect from grit to physical activity than males, while the effect between procrastination and grit was more significant for the younger. The moderated mediating model provided a new strategy of improving personality traits which contributed to lack of physical activity. More psychologically relevant measures should be taken in reducing procrastination to improve physical activity via improving grit.
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http://dx.doi.org/10.1080/07448481.2022.2068962DOI Listing
May 2022

Path-Dependent Anisotropic Colloidal Assembly of Magnetic Nanocomposite-Protein Complexes.

Langmuir 2022 May 12. Epub 2022 May 12.

The Institute for Translational Nanomedicine, Shanghai East Hospital, the Institute for Biomedical Engineering and Nano Science, School of Medicine, Tongji University, Shanghai 200092, P. R. China.

Anisotropic self-assembly of nanoparticles (NPs) stems from the fine-tuning of their surface functionality and NP interaction. Strategies involving ligand interaction, protein interaction, and external stimulus have been developed. However, robust construction of monodispersed magnetic NPs to tens of microns of anisotropically aligned colloidal assembly triggered by adsorbed protein intermolecular interaction is yet to be elucidated. Here, we present the NP-protein interaction, magnetic force, and protein corona intermolecular interaction serially but independently induced path-dependent self-assembly of 100 nm [email protected] nanocomposites. Dynamic formation of the micron-sized anisotropic magnetic assembly was reproducibly realized in a continuous medium in a controllable manner. Formation of the primary globular clusters upon the unique NP-protein complexes with the help of ions acts as the prerequisite for the anisotropic colloidal assembly, followed by the magnetic force-driven pre-organization and protein intermolecular electrostatic interaction-mediated elongation. The protein concentration rather than the protein original structure plays a more pivotal role in the NP-protein interaction and subsequent colloidal assembly process. Two typical serum proteins fibrinogen and bovine serum albumin enable formation of the anisotropic colloidal assembly but with a different subtle morphology. Furthermore, the obtained micron-sized magnetic colloidal assembly can be dissociated rapidly by adding a negative electrolyte in the medium due to the interference in the NP-protein interaction. However, the self-assembly process can be recycled based on the dissociated colloidal assembly.
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http://dx.doi.org/10.1021/acs.langmuir.1c02923DOI Listing
May 2022

Dexterous Manipulation for Multi-Fingered Robotic Hands With Reinforcement Learning: A Review.

Front Neurorobot 2022 25;16:861825. Epub 2022 Apr 25.

Institute of Automation, Chinese Academy of Sciences, Beijing, China.

With the increasing demand for the dexterity of robotic operation, dexterous manipulation of multi-fingered robotic hands with reinforcement learning is an interesting subject in the field of robotics research. Our purpose is to present a comprehensive review of the techniques for dexterous manipulation with multi-fingered robotic hands, such as the model-based approach without learning in early years, and the latest research and methodologies focused on the method based on reinforcement learning and its variations. This work attempts to summarize the evolution and the state of the art in this field and provide a summary of the current challenges and future directions in a way that allows future researchers to understand this field.
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http://dx.doi.org/10.3389/fnbot.2022.861825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083362PMC
April 2022

Incidence, Etiology, and Environmental Risk Factors of Community-Acquired Pneumonia Requiring Hospitalization in China: A 3-Year, Prospective, Age-Stratified, Multicenter Case-Control Study.

Open Forum Infect Dis 2021 Nov 6;8(11):ofab499. Epub 2021 Oct 6.

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Background: Community-acquired pneumonia (CAP) is a leading infectious cause of hospitalization and death worldwide. Knowledge about the incidence and etiology of CAP in China is fragmented.

Methods: A multicenter study performed at 4 hospitals in 4 regions in China and clinical samples from CAP patients were collected and used for pathogen identification from July 2016 to June 2019.

Results: A total of 1674 patients were enrolled and the average annual incidence of hospitalized CAP was 18.7 (95% confidence interval, 18.5-19.0) cases per 10000 people. The most common viral and bacterial agents found in patients were respiratory syncytial virus (19.2%) and (9.3%). The coinfections percentage was 13.8%. Pathogen distribution displayed variations within age groups as well as seasonal and regional differences. The severe acute respiratory syndrome coronavirus 2 was not detected. Respiratory virus detection was significantly positively correlated with air pollutants (including particulate matter ≤2.5 µm, particulate matter ≤10 µm, nitrogen dioxide, and sulfur dioxide) and significantly negatively correlated with ambient temperature and ozone content; bacteria detection was opposite.

Conclusions: The hospitalized CAP incidence in China was higher than previously known. CAP etiology showed that differences in age, seasons, regions, and respiratory viruses were detected at a higher rate than bacterial infection overall. Air pollutants and temperature have an influence on the detection of pathogens.
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http://dx.doi.org/10.1093/ofid/ofab499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522381PMC
November 2021
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