Publications by authors named "Peng Shi"

592 Publications

Estrogen-regulated AGR3 activates the estrogen receptor signaling pathway to promote tamoxifen resistance in breast cancer.

Breast Cancer Res Treat 2021 Sep 14. Epub 2021 Sep 14.

Department of Breast and Thyroid Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No. 324 Jingwuweiqi Road, Huaiyin District, Jinan, 250000, China.

Purpose: Anterior gradient 3 (AGR3) is associated with breast cancer progression, but its relationship with estrogen and tamoxifen resistance in breast cancer is still unclear. This study was designed to investigate the correlation of ARG3 and estrogen as well as the roles of ARG3 in tamoxifen resistance in breast cancer.

Methods: Online database including GEPIA, UALCAN, and TCGA and rVista predictive tool were applied to analyze the expression patterns of AGR3 and its relationship with estrogen receptor 1. AGR3 knockdown and overexpression cell models were constructed. Luciferase reporter assay and ChIP were performed to investigate intermolecular interactions. Western blotting and qPCR were applied to assess targets at mRNA and protein levels, respectively. Cell counting and MTT assay were applied to determine the cell proliferation.

Results: An elevation of AGR3 was observed in patients with breast cancer, especially in the patients with estrogen receptor (ER)-positive breast cancer. The TCGA dataset and in vitro data supported that AGR3 was positively correlated to ER. Further results demonstrated that ER protein bound to AGR3 promoter sites. AGR3 expression exhibited a positive correlation to cell viability. Besides, AGR3 promoted tamoxifen resistance in breast cancer.

Conclusion: AGR3 is associated with estrogen and promotes tamoxifen resistance in breast cancer.
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http://dx.doi.org/10.1007/s10549-021-06385-3DOI Listing
September 2021

Phenotypic and genomic adaptations to the extremely high elevation in plateau zokor (Myospalax baileyi).

Mol Ecol 2021 Sep 12. Epub 2021 Sep 12.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.

The evolutionary outcomes of high-elevation adaptation have been extensively described. However, whether widely distributed high-elevation endemic animals adopt uniform mechanisms during adaptation to different elevational environments remains unknown, especially with respect to extreme high elevation environments. To explore this, we analyze phenotypic and genomic data of seven populations of plateau zokor (Myospalax baileyi) along elevations ranging from 2700 to 4300 m. Based on whole-genome sequencing data and demographic reconstruction of the evolutionary history, we show that two populations of plateau zokor living at elevations exceeding 3700 m diverged from other populations nearly ten thousand years ago. Further, phenotypic comparisons reveal stress-dependent adaptation, as two populations living at elevations exceeding 3700 m have elevated ratios of heart mass to body mass relative to other populations, and the highest population (4300 m) displays alterations in erythrocytes. Correspondingly, genomic analysis of selective sweeps indicates that positive selection might contribute to the observed phenotypic alterations in these two extremely high-elevation populations, with the adaptive cardiovascular phenotypes of both populations possibly evolving under the functional constrains of their common ancestral population. Taken together, phenotypic and genomic evidence demonstrates that heterogeneous stressors impact adaptations to extreme elevations and reveals stress-dependent and genetically constrained adaptation to hypoxia, collectively providing new insights into the high-elevation adaptation.
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http://dx.doi.org/10.1111/mec.16174DOI Listing
September 2021

Adaptive Learning Control of Switched Strict-Feedback Nonlinear Systems With Dead Zone Using NN and DOB.

IEEE Trans Neural Netw Learn Syst 2021 Sep 8;PP. Epub 2021 Sep 8.

This article investigates the adaptive learning control for a class of switched strict-feedback nonlinear systems with external disturbances and input dead zone. To handle unknown nonlinearity and compound disturbances, a collaborative estimation learning strategy based on neural approximation and disturbance observation is proposed, and the adaptive neural switched control scheme is studied in a dynamic surface control framework. In the adaptive learning control design, to obtain the evaluation information of uncertain learning, the prediction error is constructed based on the composite learning scheme. Then, the prediction error and the compensated tracking error are applied to construct the adaptive laws of switched neural weights and switched disturbance observers. The system stability analysis is carried out through the Lyapunov approach, where the switching signal with average dwell time is considered. Through the simulation test, the effectiveness of the proposed adaptive learning controller is verified.
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http://dx.doi.org/10.1109/TNNLS.2021.3106781DOI Listing
September 2021

Risk assessments of emerging contaminants in various waters and changes of microbial diversity in sediments from Yangtze River chemical contiguous zone, Eastern China.

Sci Total Environ 2021 Aug 28;803:149982. Epub 2021 Aug 28.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.

Over recent decades, increasing chemical contamination has greatly affected aquatic life and human health, even though most contaminants are present at low concentrations. The large-scale chemical industrial parks (CIPs) concentrated in the Yangtze River Delta account for over half of the total in China, and Jiangsu Province occupies one fifth of the Yangtze River Delta. Inevitably, the ecosystems could be affected by these CIPs. In this study, we collected 35 water and 12 sediment samples from the Yangtze River (Taizhou section) surrounding waters adjacent to concentrated CIPs and determined their cumulative chemical levels to be 0.2 to 28.4 μg/L and cumulative detections to be 11 to 39 contaminants with a median of 20 contaminants. 61 out of 153 screened chemicals were detected from at least one sampling site, and 6 contaminants, mostly semi-volatile organic compounds, appeared at all sites. Among these detected chemicals, di-n-octyl phthalate and dibutyl phthalate were at the highest levels. Ecological assessment revealed that 4-chloroaniline, phenol and dibutyl phthalate possibly would induce adverse effects on Yangtze River (Taizhou) ecosystems. Further aided with an evaluation of integrated biomarker response (IBR) index, it was found that site W06 (downstream of Binjiang CIP wastewater inlet) was the location in greatest need of urgent action. As a result, the microbial diversity of sediments in the Yangtze River mainstream was significantly higher than that of tributaries, where CIPs wastewater entered.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149982DOI Listing
August 2021

Visible light-induced C-C bond cleavage in a multicomponent reaction cascade allowing acylations of sulfoximines with ketones.

Org Biomol Chem 2021 Sep 6. Epub 2021 Sep 6.

Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52074 Aachen, Germany.

Visible light induces C-C-bond cleavage reactions of ketones, which can be utilized for -acylations of sulfoximines. No (photo)catalyst is required, and the reactions occur at ambient temperature in air. The substrate scope is broad for both ketones and sulfoximines. For converting H-sulfoximines, the presence of NBS is essential.
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http://dx.doi.org/10.1039/d1ob01411kDOI Listing
September 2021

A New World Monkey Resembles Human in Bitter Taste Receptor Evolution and Function via a Single Parallel Amino Acid Substitution.

Mol Biol Evol 2021 Sep 1. Epub 2021 Sep 1.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.

Bitter taste receptors (Tas2Rs) serve as a vital component in the defense system against toxin intake by animals, and the family of genes encoding these receptors has been demonstrated, usually by family size variance, to correlate with dietary preference. However, few systematic studies of specific Tas2R to unveil their functional evolution have been conducted. Here, we surveyed Tas2R16 across all major clades of primates, which represent diverse feeding ecologies, and observed a rare case of a convergent change to increase sensitivity to β-glucopyranosides in human and a New World monkey, the white-faced saki (Pithecia pithecia). We combined evolutionary, 3D modeling and functional assay analyses to demonstrate that a parallel amino acid substitution (K172N) shared by these two species is responsible for this functional convergence of Tas2R16. Considering the specialized feeding preference of the white-faced saki, the K172N change likely played an important adaptive role in its early evolution to avoid potentially toxic cyanogenic glycosides, as suggested for the human TAS2R16 gene.
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http://dx.doi.org/10.1093/molbev/msab263DOI Listing
September 2021

Event-Triggered Control for Markov Jump Systems Subject to Mismatched Modes and Strict Dissipativity.

IEEE Trans Cybern 2021 Sep 1;PP. Epub 2021 Sep 1.

In order to save network resources of discrete-time Markov jump systems, an event-triggered control framework is employed in this article. The threshold parameter in the event-triggered mechanism is designed as a diagonal matrix in which all elements can be adjusted according to system performance requirements. The hidden Markov model is introduced to characterize the asynchronization between the controller and controlled system. The effect of randomly occurring gain fluctuations is taken into account during the controller design. For the purpose of guaranteeing that the closed-loop system is stochastically stable and satisfies the strictly (D₁,D₂,D₃)-ɣ-dissipative performance, sufficient conditions are constructed by employing the Lyapunov function and stochastic analysis. After linearization, the proposed controller gains are obtained by solving the linear matrix inequalities. Ultimately, a practical example of the dc motor device is used to illustrate the effectiveness of the proposed new design technique.
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http://dx.doi.org/10.1109/TCYB.2021.3105179DOI Listing
September 2021

Mapping Drug-Induced Neuropathy through In-Situ Motor Protein Tracking and Machine Learning.

J Am Chem Soc 2021 Sep 1;143(36):14907-14915. Epub 2021 Sep 1.

Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.

Chemotherapy can induce toxicity in the central and peripheral nervous systems and result in chronic adverse reactions that impede continuous treatment and reduce patient quality of life. There is a current lack of research to predict, identify, and offset drug-induced neurotoxicity. Rapid and accurate assessment of potential neuropathy is crucial for cost-effective diagnosis and treatment. Here we report dynamic near-infrared upconversion imaging that allows intraneuronal transport to be traced in real time with millisecond resolution, but without photobleaching or blinking. Drug-induced neurotoxicity can be screened prior to phenotyping, on the basis of subtle abnormalities of kinetic characteristics in intraneuronal transport. Moreover, we demonstrate that combining the upconverting nanoplatform with machine learning offers a powerful tool for mapping chemotherapy-induced peripheral neuropathy and assessing drug-induced neurotoxicity.
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http://dx.doi.org/10.1021/jacs.1c07312DOI Listing
September 2021

Extended high-frequency audiometry in healthy adults with different age groups.

J Otolaryngol Head Neck Surg 2021 Aug 26;50(1):52. Epub 2021 Aug 26.

Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: It was well-documented that extended high-frequency (EHF, above 8 kHz) hearing test could be more sensitive comparing with the conventional measurement on frequency below 8 kHz, regarding the early prediction of auditory damage in certain population. However, hardly any age-specific thresholds of EHF in population with normal hearing ability were observed. This study aims to monitor the age-dependent hearing thresholds at EHF (from 9 to 20 kHz) in healthy hearing population.

Methods: A total of 162 healthy participants (from 21 to 70 years) with normal conventional pure tone audiograms were recruited and separated into five groups by age. Conventional pure tone average was performed with frequencies from 0.25 to 8 kHz under air conduction and from 0.25 to 4 kHz under bone conduction. EHF audiometry from 9 to 20 kHz was determined under air conduction.

Results: The effects of aging on hearing were evident at frequencies above 4 kHz. The hearing thresholds of EHF were less than 26 dB HL before 30 years-olds. Hearing abilities in EHF were deteriorated starting from the 31 ~ 40 group and were most obvious in the 51 ~ 60 group and the 61 ~ 70 group with the maximum thresholds of 75 dB HL. Sensitivity of EHF was inversely proportional to the frequency within each age group, and to age among groups. Subjects under 30 years old were totally responsive up to 16 kHz, and 52.2% could respond to 20 kHz. Meanwhile, no responsiveness was recorded to 20 kHz in the 51 ~ 60 group and even to 18 kHz in the 61 ~ 70 group. No gender differences in hearing threshold was observed within each age group, except an obvious decline at frequencies of 4, 6, 8, and 9 kHz in male participants of the 41 ~ 50 group.

Conclusions: Hearing thresholds at EHF from 9 to 20 kHz were more sensitive than at frequencies below 8 kHz for hearing measurement, and aging inversely affected hearing ability at EHF in healthy population. Hearing thresholds at EHF deteriorated with age and raising frequency, while the upper frequency limit decreased with aging.
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http://dx.doi.org/10.1186/s40463-021-00534-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8394048PMC
August 2021

Screen-Printed Flexible Thermoelectric Device Based on Hybrid Silver Selenide/PVP Composite Films.

Nanomaterials (Basel) 2021 Aug 11;11(8). Epub 2021 Aug 11.

Key Laboratory of Instrumentation Science and Dynamic Measurement, Ministry of Education, North University of China, Taiyuan 030051, China.

In recent years, the preparation of flexible thermoelectric generators by screen printing has attracted wide attention due to easy processing and high-volume production. In this work, we propose an n-type AgSe/polymer polyvinylpyrrolidone (PVP) film based on screen printing and investigate the effect of PVP on thermoelectric performance by varying the ratio of PVP. When the content ratio of AgSe to PVP is 30:1, i.e., PI30, the fabricated PI30 film has the best thermoelectric property. The maximum power factor (PF) of the PI30 is 4.3 μW·m·K, and conductivity reaches 81% of its initial value at 1500 bending cycles. Then, the film thermoelectric generator (F-TEG) fabricated by PI30 is tested for practical application; the output voltage and the maximum output power are 21.6 mV and 233.3 nW at the temperature difference of 40 K, respectively. This work demonstrates that the use of PVP combined with screen printing to prepare F-TEG is a simple and rapid method, which provides an efficient preparation solution for the development of environmentally friendly and wearable flexible thermoelectric devices.
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http://dx.doi.org/10.3390/nano11082042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401139PMC
August 2021

High-Performance Temperature Sensor by Employing Screen Printing Technology.

Micromachines (Basel) 2021 Aug 2;12(8). Epub 2021 Aug 2.

State Key Laboratory for Mechanical Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710000, China.

In the present study, a high-performance n-type temperature sensor was developed by a new and facile synthesis approach, which could apply to ambient temperature applications. As impacted by the low sintering temperature of flexible polyimide substrates, a screen printing technology-based method to prepare thermoelectric materials and a low-temperature heat treatment process applying to polymer substrates were proposed and achieved. By regulating the preparation parameters of the high-performance n-type indium oxide material, the optimal proportioning method and the post-treatment process method were developed. The sensors based on thermoelectric effects exhibited a sensitivity of 162.5 μV/°C, as well as a wide range of temperature measurement from ambient temperature to 223.6 °C. Furthermore, it is expected to conduct temperature monitoring in different scenarios through a sensor prepared in masks and mechanical hands, laying a foundation for the large-scale manufacturing and widespread application of flexible electronic skin and devices.
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http://dx.doi.org/10.3390/mi12080924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400255PMC
August 2021

DNMT1-Induced miR-152-3p Suppression Facilitates Cardiac Fibroblast Activation in Cardiac Fibrosis.

Cardiovasc Toxicol 2021 Aug 23. Epub 2021 Aug 23.

Department of Anesthesiology, The Second Hospital of Anhui Medical University, Hefei, 230601, China.

Novel insights into epigenetic control of cardiac fibrosis are now emerging. Cardiac fibroblasts (CFs) activation into myofibroblasts and the production of extracellular matrix (ECM) is the key to cardiac fibrosis development, but the specific mechanism is not fully understood. In the present study, we found that DNMT1 hypermethylation reduces the expression of microRNA-152-3p (miR-152-3p) and promotes Wnt1/β-catenin signaling pathway leading to CFs proliferation and activation. Cardiac fibrosis was produced by ISO, and the ISO was carried out according to the method described. CFs were harvested and cultured from SD neonatal rats and stimulated with TGF-β1. Importantly, DNMT1 resulted in the inhibition of miR-152-3p in activated CFs and both DNMT1 and miR-152-3p altered Wnt/β-catenin downstream protein levels. Over expression of DNMT1 and miR-152-3p inhibitors promotes proliferation of activating CFs. In addition, decreased methylation levels and over expression of miR-152-3p inhibited CFs proliferation. We determined that DNMT1 can methylate to miR-152-3p and demonstrated that expression of miR-152-3p inhibits CFs proliferation by inhibiting the Wnt1/β-catenin pathway. Our results stand out together DNMT1 methylation regulates miR-152-3p to slow the progression of cardiac fibrosis by inhibiting the Wnt1/β-catenin pathway.
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http://dx.doi.org/10.1007/s12012-021-09690-xDOI Listing
August 2021

Reclaiming Inactive Lithium with a Triiodide/Iodide Redox Couple for Practical Lithium Metal Batteries.

Angew Chem Int Ed Engl 2021 Aug 20. Epub 2021 Aug 20.

Beijing Key Laboratory of Green Chemical Reaction Engineering and Institution, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, P. R. China.

High-energy-density lithium (Li) metal batteries suffer from a short lifespan owing to apparently ceaseless inactive Li accumulation, which is accompanied by the consumption of electrolyte and active Li reservoir, seriously deteriorating the cyclability of batteries. Herein, a triiodide/iodide (I /I ) redox couple initiated by stannic iodide (SnI ) is demonstrated to reclaim inactive Li. The reduction of I converts inactive Li into soluble LiI, which then diffuses to the cathode side. The oxidation of LiI by the delithiated cathode transforms cathode into the lithiation state and regenerates I , reclaiming Li ion from inactive Li. The regenerated I engages the further redox reactions. Furthermore, the formation of Sn mitigates the corrosion of I on active Li reservoir sacrificially. In working Li | LiNi Co Mn O batteries, the accumulated inactive Li is significantly reclaimed by the reversible I /I redox couple, improving the lifespan of batteries by twice. This work initiates a creative solution to reclaim inactive Li for prolonging the lifespan of practical Li metal batteries.
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http://dx.doi.org/10.1002/anie.202110589DOI Listing
August 2021

Bayesian Hierarchical Factor Analysis for Efficient Estimation across Race/Ethnicity.

Rev Colomb Estad 2021 Jul 12;44(2):313-329. Epub 2021 Jul 12.

Department of Biostatistics & Data Science, University of Kansas Medical Center.

Patient reported outcomes are gaining more attention in patient-centered health outcomes research and quality of life studies as important indicators of clinical outcomes, especially for patients with chronic diseases. Factor analysis is ideal for measuring patient reported outcomes. If there is heterogeneity in the patient population and when sample size is small, differential item functioning and convergence issues are challenges for applying factor models. Bayesian hierarchical factor analysis can assess health disparity by assessing for differential item functioning, while avoiding convergence problems. We conducted a simulation study and used an empirical example with American Indian minorities to show that fitting a Bayesian hierarchical factor model is an optimal solution regardless of heterogeneity of population and sample size.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356675PMC
July 2021

Cochlear hair cells of echolocating bats are immune to intense noise.

J Genet Genomics 2021 Jul 6. Epub 2021 Jul 6.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China; Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. Electronic address:

Exposure to intense noise can damage cochlear hair cells, leading to hearing loss in mammals. To avoid this constraint, most mammals have evolved in relatively quiet environments. Echolocating bats, however, are naturally exposed to continuous intense sounds from their own and neighboring sonar emissions for maintaining sonar directionality and range. Here, we propose the presence of intense noise resistance in cochlear hair cells of echolocating bats against noise-induced hearing loss (NIHL). To test this hypothesis, we performed noise exposure experiments for laboratory mice, one nonecholocating bat species, and five echolocating bat species. Contrary to nonecholocating fruit bats and mice, the hearing and the cochlear hair cells of echolocating bats remained unimpaired after continuous intense noise exposure. The comparative analyses of cochleae transcriptomic data showed that several genes protecting cochlear hair cells from intense sounds were overexpressed in echolocating bats. Particularly, the experimental examinations revealed that ISL1 overexpression significantly improved the survival of cochlear hair cells. Our findings support the existence of protective effects in cochlear hair cells of echolocating bats against intense noises, which provides new insight into understanding the relationship between cochlear hair cells and intense noises, and preventing or ameliorating NIHL in mammals.
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http://dx.doi.org/10.1016/j.jgg.2021.06.007DOI Listing
July 2021

Enhancing continuous reactive crystallization of lithium carbonate in multistage mixed suspension mixed product removal crystallizers with pulsed ultrasound.

Ultrason Sonochem 2021 Sep 2;77:105698. Epub 2021 Aug 2.

School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, People's Republic of China; The Co-Innovation Center of Chemistry and Chemical Engineering of Tianjin, Tianjin 300072, People's Republic of China. Electronic address:

In this work, pulsed ultrasound was used to facilitate steady-state reactive crystallization and increase the final yield and productivity of lithium carbonate in continuously operated single and multistage mixed suspension mixed product removal (MSMPR) crystallizers. Experimental analyses of the stirred tank MSMPR cascade were performed to investigate the effects of ultrasound field, residence time and temperature which contributed to the steady-state yield, crystal size distribution and crystal morphology. The results show that pulsed ultrasound can not only significantly enhance the reaction rate, but also help to improve the particle size distribution and the crystal habit. Subsequently, a population balance model was developed and applied to estimate the final yield of the continuous process of the lithium bicarbonate thermal decomposition reaction coupling lithium carbonate crystallization. The consistency of the final yield between the experiments and the simulations proved the reliability of the established model. Through the experimental and simulation analyses, it is demonstrated that the use of pulsed ultrasound, higher final stage temperature, MSMPR cascade design and appropriate residence time help to achieve higher yield and productivity. Furtherly, based on the conclusion drawn, pulsed ultrasound enhanced three-stage MSMPR cascaded lithium carbonate continuous crystallization processes were designed, and the maximum productivity of 44.0 g/h was obtained experimentally.
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http://dx.doi.org/10.1016/j.ultsonch.2021.105698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358474PMC
September 2021

Study on the Mechanical Properties of Fly-Ash-Based Light-Weighted Porous Geopolymer and Its Utilization in Roof-Adaptive End Filling Technology.

Molecules 2021 Jul 23;26(15). Epub 2021 Jul 23.

Key Laboratory of Deep Coal Resource Mining (CUMT), Ministry of Education of China, Xuzhou 221116, China.

This paper aims to study the porous structure and the mechanical properties of fly-ash-based light-weighted porous geopolymer (FBLPG), exploring the feasibility of using it in roof-adaptive end filling technology based on its in-situ foaming characteristics and plastic yielding performance. A porous structure model of FBLPG during both the slurry and solid period was established to study their influence factor. In addition, this study also built a planar structure model in the shape of a honeycomb with bore walls, proving that the bore walls possess the characteristics of isotropic force. FBLPG shows a peculiar plastic yielding performance in the experiment where its stress stays stable with the gradual increase of the deformation, which can guarantee the stability of a filling body under the cycled load from the roof. At the same time, the in-situ foaming process combined with the unique filling technique can make the FBLPG filling body fully in contact with the irregular roof. This roof-adaptive end filling technology makes it a successful application in plugging the 1305 working face, which avoids problems of the low tight-connection ratio and secondary air-leakage channel resulted from the traditional filling technology, effectively improving coal production in terms of safety and high efficiency.
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http://dx.doi.org/10.3390/molecules26154450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348976PMC
July 2021

Optimizing the Properties of LaSrCrO Thin Films through Post-Annealing for High-Temperature Sensing.

Nanomaterials (Basel) 2021 Jul 11;11(7). Epub 2021 Jul 11.

Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

LaSrCrO (0.2LSCO) thin films were prepared via the RF sputtering method to fabricate thin-film thermocouples (TFTCs), and post-annealing processes were employed to optimize their properties to sense high temperatures. The XRD patterns of the 0.2LSCO thin films showed a pure phase, and their crystallinities increased with the post-annealing temperature from 800 °C to 1000 °C, while some impurity phases of CrO and SrCrO were observed above 1000 °C. The surface images indicated that the grain size increased first and then decreased, and the maximum size was 0.71 μm at 1100 °C. The cross-sectional images showed that the thickness of the 0.2LSCO thin films decreased significantly above 1000 °C, which was mainly due to the evaporation of Sr and Cr. At the same time, the maximum conductivity was achieved for the film annealed at 1000 °C, which was 6.25 × 10 S/cm. When the thin films post-annealed at different temperatures were coupled with Pt reference electrodes to form TFTCs, the trend of output voltage to first increase and then decrease was observed, and the maximum average Seebeck coefficient of 167.8 µV/°C was obtained for the 0.2LSCO thin film post-annealed at 1100 °C. Through post-annealing optimization, the best post-annealing temperature was 1000 °C, which made the 0.2LSCO thin film more stable to monitor the temperatures of turbine engines for a long period of time.
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http://dx.doi.org/10.3390/nano11071802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308239PMC
July 2021

MiR-222-3p induced by hepatitis B virus promotes the proliferation and inhibits apoptosis in hepatocellular carcinoma by upregulating THBS1.

Hum Cell 2021 Jul 17. Epub 2021 Jul 17.

Department of Hepatobiliary Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinnan, 250022, Shandong, People's Republic of China.

This study aimed to explore the role of miR-222-3p in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). MiR-222-3p expression in tumor tissues of HBV (+) or HBV (-) HCC patients and corresponding cell lines was detected by quantitative reverse transcription PCR (qRT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis was evaluated by flow cytometry. The potential targets of miR-222-3p were predicted by Targetscan, and the binding relationship between miR-222-3p and thrombospondin-1 (THBS1) was determined by luciferase reporter assay and RNA immunoprecipitation (RIP) assay. MiR-222-3p was significantly upregulated in HCC tissues and cell lines and further elevated by HBV infection. MiR-222-3p downregulation effectively inhibited the proliferation and induced the apoptosis of HBV (-) HepG2 cells, HBV (+) HepG2.2.15 cells, Huh7-V cells, and Huh7-HBV cells. In addition, miR-222-3p overexpression enhanced the proliferation of these cell lines but exhibited no obvious effect on their apoptosis. Mechanistically, miR-222-3p was directly bound to the 3'-UTR of THBS1 and acted as its competing endogenous RNA (ceRNA). Interestingly, THBS1 silencing attenuated the inhibitory effect of miR-222-3p downregulation on the proliferation of these cell lines in vitro. Our results revealed that HBV infection further increased miR-222-3p expression and promoted HCC progression via miR-222-3p-mediated THBS1 downregulation. Our findings suggest that miR-222-3p might be a potential diagnostic and therapeutic target for HCC and HBV-related HCC.
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http://dx.doi.org/10.1007/s13577-021-00577-1DOI Listing
July 2021

Distinct pathways of solid-to-solid phase transitions induced by defects: the case of dl-me-thio-nine.

IUCrJ 2021 Jul 8;8(Pt 4):584-594. Epub 2021 May 8.

School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, 300072, Tianjin 300072, People's Republic of China.

Understanding of solid-to-solid phase transition mechanisms in polymorphic systems is of critical importance for rigorous control over polymorph purity in the pharmaceutical industry to achieve the desired bioavailability and efficacy of drugs. Ubiquitous defects in crystals may play an important role in the pathways of phase transitions. However, such effects remain poorly understood. Here, the effects of crystal defects on the solid-to-solid phase transformations between dl-me-thio-nine polymorphs α and β are investigated by means of experimental and computational approaches. Thermal analyses of polycrystalline powders show two endothermic peaks in the α-to-β phase transition (and two exothermic peaks for the reverse transition), in contrast with one thermal event observed for single crystals. Variable-temperature 1D and 2D Raman spectra, as well as powder X-ray diffraction patterns, reveal the appearance of two peaks that can attributed to a two-step phase transition, and the extent of the second-step phase transition increases with milling time (or defect density). Quantification of transition kinetics unveils a remarkably higher energy barrier in the second-step phase transition than in the first, proceeding by the cooperative molecular motion pathway. The good linear fitting on the kinetic data by the Jeziorny model suggests that the second-step transition follows the nucleation and growth mechanism. Molecular dynamics simulations were also conducted to understand the role of crystal defects in the solid-state phase transition by tracking the atomic distribution and hydrogen bond lifetime during the transition. It was found that the increasing defect density hinders the propagation of cooperative molecular motion, leading to a combined transition mechanism involving both cooperative motion and nucleation and growth. This study highlights the significant impact of crystal defects on solid-state phase transitions, and the two-step transition mechanism postulated may be universal given the ubiquitous presence of defects in crystalline materials.
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http://dx.doi.org/10.1107/S2052252521004401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256715PMC
July 2021

Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay.

Front Pediatr 2021 25;9:659164. Epub 2021 Jun 25.

Infectious Disease Department, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China.

To evaluate the diagnostic value of a high-throughput gene targeted amplicon sequencing (TAS) assay for detecting pathogenic microorganisms in alveolar lavage fluid (ALF) from children with severe community-acquired pneumonia (SCAP). A retrospective study was performed on 48 frozen ALF samples from 47 severe pneumonia cases admitted to Children's Hospital of Fudan University from January 1, 2019, to March 31, 2019. All samples were tested by a multiplex PCR (Multi-PCR) assay and a TAS assay. The results of the TAS panels were parallel compared with Multi-PCR and Conventional Tests (CT) including culture, direct fluorescent antibody method (DFA), and singleplex polymerase chain reaction (PCR). The proportion of pathogens detection by CT was 81.2% (39/48). The 8 common respiratory viruses including respiratory syncytial virus (RSV), adenovirus (ADV), influenza A virus (FLUA), influenza B virus (FLUB), parainfluenza virus 1-3 (PIV1-3), and human Metapneumovirus (hMPV) were found in 31.2% (15/48) of the 48 samples by DFA. With the criteria of CT results used as "Golden Standard" for determing of TAS results, the proportion of pathogens detection by TAS was 70.8% (34/48). The difference of proportion of pathogens detection between TAS and CT was not statistically significant ( = 0.232). The sensitivity and specificity of TAS for pathogens detection based on CT were 87.1% (95% CI, 71.77-95.18%) and 100.0% (95% CI, 62.88-100%), the positive predictive value (PPV) and negative predictive value (NPV) were 100.0% (95% CI, 87.35-100%) and 64.2% (95% CI, 35.62-86.02%), respectively. While Multi-PCR results were used as "Golden Standard," the total pathogens detection rate of TAS was 83.3% (40/48), which had a significant difference with that of Multi-PCR ( = 0.003). The sensitivity and PPV of TAS compared with Multi-PCR were 83.3% (95% CI, 69.23-92.03%) and 100.0% (95% CI, 89.08-100%), respectively. High rates of co-infection were proved by CT, Multi-PCR, and TAS. Mycoplasma pneumoniae (MP) and ADV were the two most frequently detected pathogens in all three assays. Compared with the CT and Multi-PCR methods, this TAS assay had a good performance in detecting bacteriological and viral pathogens from ALF. More research is needed to establish interpretation criteria based on TAS reads or analysis platforms.
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http://dx.doi.org/10.3389/fped.2021.659164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267249PMC
June 2021

Upgrade of far infrared three-wave polarimeter-interferometer system on Joint-TEXT.

Rev Sci Instrum 2021 May;92(5):053527

International Joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics, State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

The far infrared polarimeter-interferometer system (POLARIS) on the Joint-TEXT has been upgraded recently. A new support structure has been applied to the mixer array of the POLARIS for adjusting the position of the mixers precisely. Five detection chords have been added to the system on the low field side by optimizing the utilization efficiency of the laser beams on both sides of the beam splitting grid. The spatial resolution can be improved from 3 to 1.5 cm. A better measurement on plasma electron density and current density has been obtained after the system is upgraded. The initial experimental result will be introduced along with the upgrade details.
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http://dx.doi.org/10.1063/5.0040486DOI Listing
May 2021

Loss of Hepatic Angiotensinogen Attenuates Sepsis-Induced Myocardial Dysfunction.

Circ Res 2021 Aug 9;129(5):547-564. Epub 2021 Jul 9.

Departments of Cardiology (J.R., H.Z., L.N., P.S., Y.X., J.W.), Zhejiang University School of Medicine, Hangzhou, China.

[Figure: see text].
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http://dx.doi.org/10.1161/CIRCRESAHA.120.318075DOI Listing
August 2021

Rate-dependent conduction block of mitral isthmus was possibly due to the re-conduction of Ligament of Marshall.

Pacing Clin Electrophysiol 2021 Sep 10;44(9):1631-1635. Epub 2021 Jul 10.

Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

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http://dx.doi.org/10.1111/pace.14306DOI Listing
September 2021

Initial boundary value problem for a class of p-Laplacian equations with logarithmic nonlinearity.

Math Biosci Eng 2021 May;18(4):3957-3976

School of Date Science and Information Engineering, Guizhou Minzu University, Guiyang 550025, China.

In this paper, we discuss global existence, boundness, blow-up and extinction properties of solutions for the Dirichlet boundary value problem of the $ p $-Laplacian equations with logarithmic nonlinearity $ u_{t}-{\rm{div}}(|\nabla u|^{p-2}\nabla u)+\beta|u|^{q-2}u = \lambda |u|^{r-2}u\ln{|u|} $, where $ 1 < p < 2 $, $ 1 < q\leq2 $, $ r > 1 $, $ \beta, \lambda > 0 $. Under some appropriate conditions, we obtain the global existence of solutions by means of the Galerkin approximations, then we prove that weak solution is globally bounded and blows up at positive infinity by virtue of potential well theory and the Nehari manifold. Moreover, we obtain the decay estimate and the extinction of solutions.
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http://dx.doi.org/10.3934/mbe.2021198DOI Listing
May 2021

Association between epidural analgesia and indications for intrapartum caesarean delivery in group 1 of the 10-group classification system at a tertiary maternity hospital, Shanghai, China: a retrospective cohort study.

BMC Pregnancy Childbirth 2021 Jun 29;21(1):464. Epub 2021 Jun 29.

Department of Anesthesiology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, No. 2699 W Gaoke Rd, Shanghai, 201024, China.

Background: In this study, we aimed to determine whether epidural analgesia affects the indications for intrapartum caesarean delivery, such as foetal distress, dystocia, or maternal request, in nulliparous term women with spontaneous labour (Group 1 in the 10-Group Classification System).

Methods: We conducted a retrospective cohort study and collected data from the electronic medical records of deliveries performed in our institution between 1 January 2017 and 30 June 2017. Women conforming to the criterion of Group 1 according to the 10-Group Classification System were enrolled. We compared labour outcomes between women with and without epidural analgesia and analysed the association between epidural analgesia and indications for caesarean by using multivariate logistic regression analysis.

Results: A total of 3212 women met the inclusion criteria, and 2876 were enrolled in the final analyses. Women who received epidural analgesia had a significantly lower intrapartum caesarean delivery rate (16.0% vs. 26.7%, P < 0.001), higher rates of amniotomy (53.4% vs. 42.3%, P < 0.001) and oxytocin augmentation (79.5% vs. 67.0%, P < 0.001), and a higher incidence of intrapartum fever (≥38 °C) (23.3% vs. 8.5%, P < 0.001) than those who did not receive epidural analgesia. There were no significant differences between the groups for most indications, except a lower probability of maternal request for caesarean delivery (3.9% vs. 10.5%, P < 0.001) observed in women who received epidural analgesia than in those who did not. Epidural analgesia was revealed to be associated with a decreased risk of maternal request for caesarean delivery (adjusted odds ratio [aOR], 0.30; 95% confidence interval [CI], 0.22-0.42; P < 0.001); however, oxytocin augmentation was related to an increased risk of maternal request (aOR, 2.34; 95%CI, 1.47-3.75; P < 0.001). Regarding the reasons for the maternal request for caesarean delivery, significantly fewer women complained of pain (0.5% vs. 4.6%, P < 0.001) or had no labour progress (1.3% vs. 3.6%, P < 0.001) among those who received analgesia.

Conclusions: Among the women in Group 1, epidural analgesia was associated with a lower intrapartum caesarean delivery rate, which may be explained by a reduction in the risk of maternal request for an intrapartum caesarean delivery.
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http://dx.doi.org/10.1186/s12884-021-03925-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243735PMC
June 2021

Targeted Discovery of the Polyene Macrolide Hexacosalactone A from Streptomyces by Reporter-Guided Selection of Fermentation Media.

J Nat Prod 2021 07 25;84(7):1924-1929. Epub 2021 Jun 25.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, People's Republic of China.

New approaches are still needed to fully explore the biosynthetic potential of microbes. We recently devised a reporter-guided fermentation media screening approach for targeted activation of cryptic gene clusters. Using this approach, we successfully activated the expression of the gene cluster in sp. LZ35 and discovered a novel polyene macrolide hexacosalactone A ().
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http://dx.doi.org/10.1021/acs.jnatprod.1c00144DOI Listing
July 2021

Echolocation in soft-furred tree mice.

Science 2021 06;372(6548)

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.

Echolocation is the use of reflected sound to sense features of the environment. Here, we show that soft-furred tree mice () echolocate based on multiple independent lines of evidence. Behavioral experiments show that these mice can locate and avoid obstacles in darkness using hearing and ultrasonic pulses. The proximal portion of their stylohyal bone fuses with the tympanic bone, a form previously only seen in laryngeally echolocating bats. Further, we found convergence of hearing-related genes across the genome and of the echolocation-related gene between soft-furred tree mice and echolocating mammals. Together, our findings suggest that soft-furred tree mice are capable of echolocation, and thus are a new lineage of echolocating mammals.
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http://dx.doi.org/10.1126/science.aay1513DOI Listing
June 2021

Insight into Organic Pollutant Adsorption Characteristics on a g-CN Surface by Attenuated Total Reflection Spectroscopy and Molecular Dynamics Simulation.

Langmuir 2021 Jun 15;37(25):7655-7667. Epub 2021 Jun 15.

State Key Laboratory of Pollution Control and Resource Reuse, Jiangsu Key Laboratory of Vehicle Emissions Control, School of the Environment, Nanjing University, Nanjing 210023, P. R. China.

Herein the adsorption characteristics of zwitterionic dye pollutant Rhodamine B (RhB) on a g-CN surface were investigated by both an attenuated total reflection spectroscopy (ATRS) experiment and a molecular dynamics simulation (MDS). For experimental investigation, g-CN was coated on a silica optical fiber (SOF) surface to fabricate an adsorption film. According to the ATRS response, adsorption thermodynamics and thermodynamics results were in situ obtained and evaluated. The isothermal Langmuir model was used to calculate the adsorption equilibrium constants () and adsorption energies (Δ) for RhB as 27.25 × 10 M and -31.01 kJ mol, respectively, which indicated the spontaneous adsorption behavior of RhB at the g-CN surface. Using dynamic Elovich modeling, the rate constants of RhB were found to be = 0.0063 min and = 0.0004 min, which indicated two-stage adsorption at the g-CN surface. For theoretical simulation, adsorption configurations and adsorption energies were systematically calculated by a molecular dynamics simulation (MDS) . RhB molecules were inclined to orient in a parallel position at the g-CN surface during low concentration but a perpendicular position at the g-CN surface during high concentration. Combined with experimental and calculation results, this work revealed the microscopic adsorption performance and elucidated the intermolecular interaction between localized interfaces of g-CN and hazardous dye pollutant. We propose an adsorption model to explain the process of surface interaction, which is based on molecular orientation and a force-driven mechanism. Electrostatic attraction and π-π interaction dominated the adsorption interaction with an adsorption energy of Δ = -38.96 kJ mol for low RhB concentration, and electrostatic attraction dominated the adsorption interaction with an adsorption energy of Δ = -25.76 kJ mol for high RhB concentration. This work can provide a fundamental basis for a dye-pollutants removal application by g-CN in both adsorption and photocatalyzation.
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http://dx.doi.org/10.1021/acs.langmuir.1c00360DOI Listing
June 2021

Dynamic Event-Triggered State Estimation for Markov Jump Neural Networks With Partially Unknown Probabilities.

IEEE Trans Neural Netw Learn Syst 2021 Jun 10;PP. Epub 2021 Jun 10.

This article focuses on the investigation of finite-time dissipative state estimation for Markov jump neural networks. First, in view of the subsistent phenomenon that the state estimator cannot capture the system modes synchronously, the hidden Markov model with partly unknown probabilities is introduced in this article to describe such asynchronization constraint. For the upper limit of network bandwidth and computing resources, a novel dynamic event-triggered transmission mechanism, whose threshold parameter is constructed as an adjustable diagonal matrix, is set between the estimator and the original system to avoid data collision and save energy. Then, with the assistance of Lyapunov techniques, an event-based asynchronous state estimator is designed to ensure that the resulting system is finite-time bounded with a prescribed dissipation performance index. Ultimately, the effectiveness of the proposed estimator design approach combining with a dynamic event-triggered transmission mechanism is demonstrated by a numerical example.
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http://dx.doi.org/10.1109/TNNLS.2021.3085001DOI Listing
June 2021
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