Publications by authors named "Peng Ren"

251 Publications

Transcriptome analysis of breast muscle and liver in full-sibling hybrid broilers at different ages.

Gene 2022 Aug 9:146801. Epub 2022 Aug 9.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address:

In China, the production mode of hybrid broilers with meat-type chicken as male parent and egg-type chicken as female parent is common, but few studies pay attention to the economic characteristics of hybrid broilers. In this experiment, we constructed a full-sib F1 population (n=57) from male Recursive White broiler and female Lohmann Pink layer. Total 6, 6 and 7 hybrid broilers at days 1, 28 and 56 were selected randomly to collect breast muscle and liver tissues, respectively. After performing strand-specific RNA-Seq on these samples, we obtained 252.12 Gb sequencing data. Principal component analysis presented that the effects of different factors on gene expression were as below: tissue difference > age difference > sex difference. The ten genes with the highest expression in breast muscle were GAPDH, ACTA1, ATP2B3, COII, ATP6, COX3, COX1, MYL1, TNNI2 and ENSGALG00000042024. Through the analysis of differentially expressed transcripts (DETs) between different ages, we found that the number of DETs decreased progressively with the prolongation of ages in breast muscle. The same results were also observed in liver. GO enrichment analysis of DETs demonstrated that total 11 BP terms closely related to growth and development of breast muscle were annotated, such as cardiac muscle contract, muscle contract, cell division and so on. KEGG annotation presented that total 5 pathways related to growth and development were determined in breast muscle, including Cell cycle, Insulin signaling pathway, FoxO signaling pathway, Focal adhesion and Adrenergic signaling in cardiomyocytes. Our results may provide theoretical foundation for hybrid broiler production.
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http://dx.doi.org/10.1016/j.gene.2022.146801DOI Listing
August 2022

Bioinformatics analysis reveals three key genes and four survival genes associated with youth-onset NSCLC.

Open Med (Wars) 2022 6;17(1):1123-1133. Epub 2022 Jul 6.

Department of Thoracic Surgery, Peking University Third Hospital, Haidian, Beijing 100191, China.

Youth-onset non-small cell lung cancer (NSCLC) is a heterogeneous disease. It has a unique clinicopathology and special genetic background. In this study, three key genes, CDC20, CCNB2, and BUB1, have been identified in youth-onset NSCLC tumor tissues based on the TCGA and GEO cohorts. Functional enrichment analysis reveals that the "oocyte meiosis," "cell cycle," and the "P53 signaling pathway" are significantly enriched. Additionally, four survival genes, including AKAP12, CRIM1, FEN1, and SLC7A11, that affect the prognosis of youth-onset NSCLC patients are identified in this study. Finally, we construct a risk model to predict the overall survival of youth-onset NSCLC patients, the AUC of the risk model in 1, 3, and 5 years of overall survival is 0.808, 0.844, and 0.728. This study aims to provide a novel idea to explore the pathogenic genes of youth-onset NSCLC.
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http://dx.doi.org/10.1515/med-2022-0492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263893PMC
July 2022

[Network Meta-analysis of Chinese medicine injections in treatment of hypertensive intracerebral hemorrhage].

Zhongguo Zhong Yao Za Zhi 2022 Jul;47(13):3637-3647

Gansu University of Chinese Medicine Lanzhou 730000, China Evidence-based Medical Center, Gansu University of Chinese Medicine Lanzhou 730000, China.

To systematically evaluate the efficacy and safety of Chinese medicine injections in the treatment of hypertensive intracerebral hemorrhage, we collected the relevant randomized controlled trials(RCTs) by computer retrieval from PubMed, EMbase, Cochrane Library, Web of Science, Wanfang, CNKI, VIP, and CBM within the timespan from inception to December 30, 2021. The obtained index data were analyzed by RevMan 5.3 and Stata 15.0. Finally, 63 RCTs were selected for analysis, involving 5 953 patients. The experimental groups involved 9 Chinese medicine injections, including Danshen Injection, Danhong Injection, Sodium Aescinate Injection, Qingkailing Injection, Compound Shexiang Injection, Shuxuening Injection, Yinxing Damo Injection, Ginkgolide Injection, and Xingnaojing Injection. The network Meta-analysis showcased the following trends.(1)The surface under the cumulative ranking curve(SUCRA) in improving neurological function ranked in the order of surgical operation+conventional treatment of western medicine combined with Danhong Injection>combined with Xingnaojing Injection>combined with Ginkgolide Injection>combined with Compound Shexiang Injection>combined with Danshen Injection>combined with Sodium Aescinate Injection>combined with Qingkailing Injection>combined with Shuxuening Injection>combined with Yinxing Damo Injection.(2)In terms of National Institutes of Health stroke scale(NIHSS) score, the SUCRA ranked in the order of surgical operation+conventional treatment of western medicine combined with Xingnaojing Injection>combined with Compound Shexiang Injection>combined with Yinxing Damo Injection>combined with Ginkgolide Injection>combined with Danhong Injection>combined with Sodium Aescinate Injection.(3)In terms of Glasgow coma scale(GCS) score, the ranking of SUCRA was surgical operation+conventional treatment of western medicine combined with Yinxing Damo Injection>combined with Qingkailing Injection>combined with Sodium Aescinate Injection>combined with Danhong Injection>combined with Ginkgolide Injection>combined with Xingnaojing Injection.(4)The SUCRA in volume of residual cerebral hematoma ranked in the order of surgical operation+conventional treatment of western medicine combined with Sodium Aescinate Injection>combined with Xingnaojing Injection>combined with Danhong Injection>combined with Ginkgolide Injection>combined with Shuxuening Injection>combined with Compound Shexiang Injection. The experimental group had lower incidence of adverse reactions than the control group. The results of network Meta-analysis suggest that on the basis of surgical operation+conventional treatment of western medicine, the application of Chinese medicine injections can improve the efficacy of treating hypertensive intracerebral hemorrhage. However, in view of the great differences in the quality and number of studies included for different therapies, the SUCRA of Chinese medicine injections need to be further verified with high-quality multi-center, large-sample, randomized double-blind trials.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220214.501DOI Listing
July 2022

The Effect of Neuroepo on Cognition in Parkinson's Disease Patients Is Mediated by Electroencephalogram Source Activity.

Front Neurosci 2022 30;16:841428. Epub 2022 Jun 30.

Ministry of Education (MOE) Key Lab for Neuroinformation, The Clinical Hospital of Chengdu Brain Science Institute, University of Electronic Science and Technology of China, Chengdu, China.

We report on the quantitative electroencephalogram (qEEG) and cognitive effects of Neuroepo in Parkinson's disease (PD) from a double-blind safety trial (https://clinicaltrials.gov/, number NCT04110678). Neuroepo is a new erythropoietin (EPO) formulation with a low sialic acid content with satisfactory results in animal models and tolerance in healthy participants and PD patients. In this study, 26 PD patients were assigned randomly to Neuroepo ( = 15) or placebo ( = 11) groups to test the tolerance of the drug. Outcome variables were neuropsychological tests and resting-state source qEEG at baseline and 6 months after administering the drug. Probabilistic Canonical Correlation Analysis was used to extract latent variables for the cognitive and for qEEG variables that shared a common source of variance. We obtained canonical variates for Cognition and qEEG with a correlation of 0.97. Linear Mixed Model analysis showed significant positive dependence of the canonical variate cognition on the dose and the confounder educational level ( = 0.003 and = 0.02, respectively). Additionally, in the mediation equation, we found a positive dependence of Cognition with qEEG for ( = < 0.0001) and with dose ( = 0.006). Despite the small sample, both tests were powered over 89%. A combined mediation model showed that 66% of the total effect of the cognitive improvement was mediated by qEEG ( = 0.0001), with the remaining direct effect between dose and Cognition ( = 0.002), due to other causes. These results suggest that Neuroepo has a positive influence on Cognition in PD patients and that a large portion of this effect is mediated by brain mechanisms reflected in qEEG.
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http://dx.doi.org/10.3389/fnins.2022.841428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280298PMC
June 2022

A chemosensing approach for the colorimetric and spectroscopic detection of Cr, Cu, Fe, and Gd metal ions.

Sci Total Environ 2022 Jul 9;845:157242. Epub 2022 Jul 9.

School of Science, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China. Electronic address:

Metal cations are present in domestic and industrial wastewater and have adverse effects on human and aqueous life. The present study describes the development of the molecular probe 9-anthracen-9-ylmethylene)hydrazineylidene)methyl)-2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-8-ol (AMHMPQ) to detect Cr, Cu, Fe, and Gd ions by using UV-visible, fluorescence, colorimetric and excitation-emission matrix (EEM) spectroscopy techniques. The interaction of Cr, Cu, Fe, and Gd can be observed by the absorption maxima shift, turn-off, colour changes, and EEM shifts. In addition, fluorescence limits of detection 17.66 × 10 M, 6.44 × 10 M, 28.87 × 10 M, and 12.49 × 10 M in wide linear ranges, low limits of quantifications, high values of Stern-Volmer constant, Job's plot and Benesi-Hildebrand plot justify the 1:1 association affinity with association constants of 1.46 × 10 M, 1.86 × 10 M, 2.69 × 10 M, 2.13 × 10 M for AMHMPQ-metal ions (Cr, Cu, Fe, and Gd ions), respectively. Paper- and mask-based kits are developed to explore the utility of the designed chemosensor. Additionally, AMHMPQ acts as a reusable sensor for two, seven, two, and zero cycles for Cr, Cu, Fe, and Gd ions, respectively, when checked with EDTA.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157242DOI Listing
July 2022

Sigma-1 Receptors in Depression: Mechanism and Therapeutic Development.

Front Pharmacol 2022 16;13:925879. Epub 2022 Jun 16.

Beijing Institute of Basic Medical Sciences, Beijing, China.

Depression is the most common type of neuropsychiatric illness and has increasingly become a major cause of disability. Unfortunately, the recent global pandemic of COVID-19 has dramatically increased the incidence of depression and has significantly increased the burden of mental health care worldwide. Since full remission of the clinical symptoms of depression has not been achieved with current treatments, there is a constant need to discover new compounds that meet the major clinical needs. Recently, the roles of sigma receptors, especially the sigma-1 receptor subtype, have attracted increasing attention as potential new targets and target-specific drugs due to their translocation property that produces a broad spectrum of biological functions. Even clinical first-line antidepressants with or without affinity for sigma-1 receptors have different pharmacological profiles. Thus, the regulatory role of sigma-1 receptors might be useful in treating these central nervous system (CNS) diseases. In addition, long-term mental stress disrupts the homeostasis in the CNS. In this review, we discuss the topical literature concerning sigma-1 receptor antidepressant mechanism of action in the regulation of intracellular proteostasis, calcium homeostasis and especially the dynamic Excitatory/Inhibitory (E/I) balance in the brain. Furthermore, based on these discoveries, we discuss sigma-1 receptor ligands with respect to their promise as targets for fast-onset action drugs in treating depression.
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http://dx.doi.org/10.3389/fphar.2022.925879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243434PMC
June 2022

Mammary Leukocyte-Assisted Nanoparticle Transport Enhances Targeted Milk Trace Mineral Delivery.

Adv Sci (Weinh) 2022 Jun 30:e2200841. Epub 2022 Jun 30.

Institute of Dairy Science, College of Animal Sciences, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310029, P. R. China.

Nanoparticles are applied as versatile platforms for drug/gene delivery in many applications owing to their long-retention and specific targeting properties in living bodies. However, the delivery mechanism and the beneficial effect of nanoparticle-retention in many organisms remain largely uncertain. Here, the transport and metabolism of mineral nanoparticles in mammary gland during lactation are explored. It is shown that maternal intravenous administration of iron oxide nanoparticles (IONPs; diameter: ≈11.0 nm, surface charge: -29.1 mV, surface area: 1.05 m g ) provides elevated iron delivery to mammary gland and increased iron secretion into breast milk, which is inaccessible by classical iron-ion transport approaches such as the transferrin receptor-mediated endocytic pathway. Mammary macrophages and neutrophils are found to play dominant roles in uptake and delivery of IONPs through an unconventional leukocyte-assisted iron secretion pathway. This pathway bypasses the tight iron concentration regulation of liver hepcidin-ferroportin axis and mammary epithelial cells to increase milk iron-ion content derived from IONPs. This work provides keen insight into the metabolic pathway of nanoparticles in mammary gland while offering a new scheme of nutrient delivery for neonate metabolism regulation by using nanosized nutrients.
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http://dx.doi.org/10.1002/advs.202200841DOI Listing
June 2022

[Application of free transverse gracilis myocutaneous flap for soft tissue defects of foot and ankle].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Jun;36(6):766-769

Department of Trauma and Microreconstructive Surgery, Orthopaedic Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi Xinjiang, 830054, P. R. China.

Objective: To investigate the effectiveness of free transverse gracilis myocutaneous flap for soft tissue defects of foot and ankle.

Methods: Between January 2017 and December 2020, 16 cases (17 feet) of soft tissue defects of foot and ankle were repaired with free transverse gracilis myocutaneous flaps. There were 10 males and 6 females, with an average age of 38 years (range, 23-60 years). There were 9 cases of left foot, 6 cases of right foot, and 1 case of bilateral feet. The causes of soft tissue defect were traffic accident injury in 3 cases, heavy object smash injury in 4 cases, machine injury in 3 cases, infection in 4 cases, electrical burn in 1 case, and synovial sarcoma after operation in 1 case. The wounds located at the distal plantar in 2 cases (2 feet), the heel and ankle in 6 cases (6 feet), the dorsum of the foot in 7 cases (8 feet), and the first metatarsophalangeal joint to the medial malleolus in 1 case (1 foot). The size of wounds ranged from 6 cm×5 cm to 18 cm×7 cm. The size of flap ranged from 11 cm×6 cm to 21 cm×9 cm. The donor site was sutured directly.

Results: After operation, 1 case (1 foot) of flap vascular crisis, 1 case (1 foot) of partial necrosis of the flap, and 1 case of partial dehiscence of the incision at donor site occurred, all of which healed after symptomatic treatment. The other flaps survived, and the incisions at donor and recipient sites healed by first intention. All patients were followed up 12-36 months (mean, 24 months). Except for 1 case (1 foot) of swollen flap, which underwent two-stage trimming, the other flaps had good shape and texture. All the flaps had a protective feeling. At last follow-up, Kofoed scores of foot and ankle function ranged from 73 to 98 (mean, 89.7); 13 cases were excellent, 2 cases were good, and 1 case was poor, with an excellent and good rate of 93.8%. Linear scar was formed at the donor site without adverse effect on lower limb function.

Conclusion: The free transverse gracilis myocutaneous flap is an effective flap for repairing large soft tissue defects of foot and ankle due to its advantages of large excisable area, less variation of vascular anatomy, and concealment of donor site.
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http://dx.doi.org/10.7507/1002-1892.202202065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240836PMC
June 2022

Homeobox A1 Facilitates Immune Escape and Alleviates Oxidative Stress in Lung Adenocarcinoma.

Oxid Med Cell Longev 2022 19;2022:4102666. Epub 2022 May 19.

Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117 Shandong, China.

Objective: Recent studies have demonstrated that homeobox A1 (HOXA1) is upregulated in lung cancer due to RNA modifications (N-methyladenosine), but the specific function of HOXA1 in lung adenocarcinoma (LUAD) remains indistinct. Herein, we investigated the role of HOXA1 in LUAD biology.

Methods: This study presented pancancer analysis of associations of HOXA1 with prognosis, TMB, and immune checkpoints. The expression of HOXA1 was detected in LUAD and normal tissues with immunohistochemistry and western blot. Through least absolute shrinkage and selection operator (LASSO) analysis, HOXA1-derived gene model was conducted in LUAD. Correlations of HOXA1 with immune cell infiltrations, immune checkpoints, HLAs, and chemotherapeutic sensitivity were evaluated. Colony formation, proliferation, and migration of LUAD cells with si-HOXA1 transfection were investigated, and the effects of HOXA1 on T cell exhaustion were assessed .

Results: HOXA1 expression was a risk factor of overall survival, disease-specific survival, and progression-free interval of LUAD. HOXA1 exhibited prominent associations with immune cell infiltration, immune checkpoints, and HLAs. HOXA1-derived gene signature reliably and independently predicted LUAD outcomes. Also, high-risk cases presented increased sensitivity to cisplatin, paclitaxel, docetaxel, vinorelbine, and etoposide. HOXA1 knockdown exhibited an inhibitory effect on proliferation and migration abilities of LUAD cells. Silencing HOXA1 weakened the expression of antioxidative stress markers Nrf2/HO-1 and T cell exhaustion marker CD155 in LUAD cells. Moreover, LUAD cells with HOXA1 knockdown enhanced the CD8+ T cell response.

Conclusion: Our data support the oncogenic function and prognostic significance of HOXA1 that facilitates immune escape and alleviates oxidative stress of LUAD.
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http://dx.doi.org/10.1155/2022/4102666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136634PMC
June 2022

Removal behavior and mechanism of amino/carboxylate-functionalized [email protected] for Cr(VI) and Cd(II) from aqueous solutions.

Environ Sci Pollut Res Int 2022 May 24. Epub 2022 May 24.

School of Chemical Engineering and Material Science, Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, Zhengzhou, 450002, People's Republic of China.

The novel iron-based reductive particles, functionalized with amino and carboxylic functional groups, were synthesized to remove Cr(VI) and Cd(II) ions from aqueous solutions. The morphological structure and surface functional groups of new composites were characterized with SEM, XRD, FTIR, BET, and other techniques. The influence of pH, initial concentration, adsorbent dosing, and temperature on removal efficiencies were explored by batch experiments. The adsorption capacity of Cr(VI) and Cd(II) increased by 159.95% and 76.60%, respectively, compared with Fe, reaching 47.638 and 62.047 mg/g. EDS and XPS analysis showed most of Cr(VI) was reduced to Cr(III) and precipitated as ferrochrome oxide, and Cd(II) was mainly precipitated as hydroxide. Reduction-precipitation and complexation may predominate in the removal process of Cr(VI), which fitted well with Langmuir and Freundlich models and pseudo-second-order kinetics. While hydrolysis and complexation may prevail for Cd(II), which was suited with Langmuir model and pseudo-second-order kinetics. Having good magnetic properties, the A/[email protected] particles exhibited excellent reusable stability after four times regeneration experiments, promising a prospect for in-situ remediations of groundwater contaminated by Cr(VI) and Cd(II).
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http://dx.doi.org/10.1007/s11356-022-20048-wDOI Listing
May 2022

Integrated analysis of microRNA and mRNA interactions in ovary of counter-season breeding and egg-ceased geese (Anser cygnoides).

Theriogenology 2022 Jul 18;186:146-154. Epub 2022 Apr 18.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address:

Egg-ceasing is a phenomenon that occurs in most avian species and significantly reduces productivity. Although several factors are reported to regulate the reproduction progress, the underlying molecular mechanism of egg-ceasing remains obscure. Herein, we identified and explored the differentially expressed miRNAs and mRNAs involved in ovarian atrophy via high throughput sequencing. We identified a total of 901 mRNAs and 50 miRNAs that were differentially expressed in egg-laying and atrophic ovaries. Among them, numerous differentially expressed gene (DEG) transcripts and target genes for miRNAs were significantly enriched in Gene Ontology terms such as reproductive processes, cell proliferation, and apoptosis pathways. In addition, an interaction network was constructed by considering target relationships and correlation of the expression levels between ovary development-related genes, miRNAs and pathways. We discovered mRNA and miRNAs transcripts that are candidate regulators of ovary development in egg-ceased geese. Our findings expanded our understanding of the functional of miRNAs in ovarian atrophy and demonstrated that RNA-Seq is a powerful tool for examining the molecular mechanism in regulating egg-ceasing.
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http://dx.doi.org/10.1016/j.theriogenology.2022.04.007DOI Listing
July 2022

Insight into the role of a novel c-di-GMP effector protein in Rhodococcus ruber.

Biochem Biophys Res Commun 2022 06 26;608:177-182. Epub 2022 Mar 26.

College of Life Science, Jiangxi Normal University, Nanchang, 330022, China. Electronic address:

C-di-GMP is a ubiquitous second messenger in bacterium, which regulates cellular functions such as the formation of biofilm membrane, cell mobility, virulence, cell adhesion, cell cycle et al. These functions are associated with an increasing number of c-di-GMP effector proteins and/or riboswitchs. In the study, CEP1 (c-di-GMP effector protein 1), a novel c-di-GMP binding protein, was screened with a combination of affinity pull-down and LC/MS/MS methods. The binding of CEP1 and c-di-GMP was demonstrated by surface plasmon resonance, with the dissociation constants of 127 ± 1.03 μM. Quantitative real time PCR assay showed the mRNA levels of cep1 gene in Rhodococcus ruber SD3 increased to 63.29 times and 71.18 times after toluene and phenol stress, respectively. Furthermore, cep1 gene enhanced strain was constructed using shuttle plasmid pNV18, which showed improved growth compared to the wild-type strain in the presence of different organic solvents. The study provided an insight into a mechanism, by which c-di-GMP was connected with organic solvent tolerance of Rhodococcus ruber SD3.
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http://dx.doi.org/10.1016/j.bbrc.2022.03.131DOI Listing
June 2022

Treatment of Severe Refractory Thrombocytopenia in Brucellosis with Eltrombopag: A Case Report.

Pediatr Infect Dis J 2022 08 13;41(8):e332-e335. Epub 2022 Jul 13.

Department of Hematology, Kunming Children's Hospital, Kunming, China.

Brucellosis is a zoonotic disease. Severe refractory thrombocytopenia caused by brucellosis is very rare and easily misdiagnosed. We reported a 5-year-old girl who developed severe refractory thrombocytopenia secondary to brucellosis. The first-line treatment including corticosteroids and intravenous immunoglobulin did not elevate her platelets, but eltrombopag worked well and her platelet count recovered rapidly.
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http://dx.doi.org/10.1097/INF.0000000000003555DOI Listing
August 2022

Efficacy comparison of trifocal bone transport using unilateral external fixator for femoral and tibial bone defects caused by infection.

BMC Surg 2022 Apr 12;22(1):141. Epub 2022 Apr 12.

Department of Trauma and Microreconstructive Surgery, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, 830054, Xinjiang, China.

Background: This study aimed to evaluate the clinical and functional outcomes of patients with femoral and tibial critical-sized bone defect (CSBD) treated by trifocal bone transport using the Ilizarov method.

Methods: From March 2011 and January 2017, clinical and radiographic data of patients with CSBD (> 6 cm) caused by infection were documented and analyzed. Patients were divided into the femur group (n = 18) and tibia groups (n = 21) according to the location of bone transport. The bone and functional outcomes were evaluated according to the Association for the Study and Application of the Method of the Ilizarov (ASAMI) criterion, and postoperative complications were evaluated by Paley classification.

Results: A total of 39 patients were managed by the trifocal bone transport for the femur (n = 18) or tibia (n = 21) bone defects with a mean follow-up time of 26.1 months (range 17-34 months). Eighteen femurs and 21 tibias with a mean distraction regenerate length (DRL) of 8.3 cm (range 6-13 cm) and 7.5 cm (range 6-11 cm) respectively. Infection was eradicated in all patients, and the total bone union was received in all cases (100%). Statistical difference of bone grade (excellent/good/fair/poor, 3/11/3/1 vs 2/13/4/2, P < 0.05), and function grade (excellent/good/fair/poor, 3/14/1/0 vs 4/13/3/1, P < 0.05) were respectively observed between the femur group and tibia group. The excellent and good rate of bone (femur vs tibia, 77.8% vs 71.4%), and function grade (femur vs tibia, 94.4% vs 80.9%) was higher in the femur group than the tibia. The rate of complication in the femur group was lower than in the tibia (femur vs tibia, 94.4% vs 76.2%). One femur and five tibias were performed additional surgery for delayed union and axial deviation.

Conclusions: The trifocal bone transport using the unilateral external fixator was a practical method in the management of CSBD in the lower extremity. The BUT and EFI of the femur group were shorter than the tibia. Although the complications noted were more frequent on the femur, these were mostly minor.
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http://dx.doi.org/10.1186/s12893-022-01586-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004006PMC
April 2022

Stability Analysis of fMRI BOLD Signals for Disease Diagnosis.

IEEE Trans Neural Syst Rehabil Eng 2022 19;30:967-978. Epub 2022 Apr 19.

Previous studies have demonstrated that the stability changes in physiological signals can reflect individuals' pathological conditions. Apart from this, according to system science theory, a large-scale system can generally be divided into many subsystems whose stability level govern its overall performance. Therefore, this study attempts to investigate the possibility of analyzing the stability of decomposed subsystems of resting-state fMRI (rs-fMRI) BOLD signals in order to assess the overall characteristic of the human brain and individuals' health conditions. We used attention deficit/hyperactive disorder (ADHD) as an example to illustrate our method. Rs-fMRI BOLD signals were first decomposed into dynamic modes (DMs) which can illuminate the patterns of brain subsystems. Each DM is associated with one eigenvalue that determines its stability as well as oscillation frequency. Accordingly, we divided the DMs within common BOLD frequency bands into stable and unstable DMs. Then, the features related to the stability of those DMs were extracted, and nine common classifiers were used to differentiate healthy controls from ADHD patients taken from ADHD-200, a well-known dataset. The results showed that almost all features were statistically significant. Additionally, our proposed approach outperforms all existing methods with the highest possible precision, recall, and area under the receiver operating characteristic curve of 100%. In sum, we are the first to evaluate the stability of BOLD signals and demonstrate its possibility for disease diagnosis. This method can unveil new mechanisms of brain function, and could be widely used in medicine and engineering.
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http://dx.doi.org/10.1109/TNSRE.2022.3164074DOI Listing
April 2022

Circadian Rhythm and Neurotransmitters Are Potential Pathways through Which Ocean Acidification and Warming Affect the Metabolism of Thick-Shell Mussels.

Environ Sci Technol 2022 04 16;56(7):4324-4335. Epub 2022 Mar 16.

College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China.

Although the impacts of ocean acidification and warming on marine organisms have been increasingly documented, little is known about the affecting mechanism underpinning their interactive impacts on physiological processes such as metabolism. Therefore, the effects of these two stressors on metabolism were investigated in thick-shell mussel in this study. In addition, because metabolism is primarily regulated by circadian rhythm and neurotransmitters, the impacts of acidification and warming on these two regulatory processes were also analyzed. The data obtained demonstrated that the metabolism of mussels (indicated by the clearance rate, oxygen consumption rate, ammonia excretion rate, O:N ratio, ATP content, activity of pyruvate kinase, and expression of metabolism-related genes) were significantly affected by acidification and warming, resulting in a shortage of energy supply (indicated by the in vivo content of ATP). In addition, exposure to acidification and warming led to evident disruption in circadian rhythm (indicated by the heartrate and the expression rhythm of , , and ) and neurotransmitters (indicated by the activity of acetyl cholinesterase and in vivo contents of ACh, GABA, and DA). These findings suggest that circadian rhythms and neurotransmitters might be potential routes through which acidification and warming interactively affect the metabolism of mussels.
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http://dx.doi.org/10.1021/acs.est.1c06735DOI Listing
April 2022

Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis.

Bioengineered 2022 03;13(3):7457-7470

Department of Anesthesiology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, Dongcheng,China.

Glioma is the most common primary malignant brain tumors in adults. Despite considerable advances in treatment, the clinical outcome remains dismal. Translocator protein 18 kDa (TSPO), an evolutionarily conserved transmembrane protein, has always been found to be elevated in glioma, which predicts a poor prognosis. However, studies on the regulatory network of TSPO in glioma are limited. The Cancer Genome Atlas (TCGA) and our research group cohorts demonstrated that TSPO expression was also highly expressed in glioma tissues and glioma cell lines. Inhibition of TSPO expression significantly reduced glioma cell proliferation and mobility . Suppression of TSPO decreased the expression of MAPK-activated protein kinase 3 (MAPKAPK3) and increased the degradation rate of its mRNA. TSPO directly interacts with ELAV1-like RNA-binding protein 1 (HUR) and promotes the nuclear-cytoplasmic shuttling of HUR. Inhibition of HUR decreased MAPKAPK3 expression and cell proliferation and mobility, whereas overexpression of MAPKAPK3 reversed the effects. Overexpression of HUR in TSPO-knockdown cells enhanced the mRNA stability of MAPKAPK3. Furthermore, rescue experiments show that the HUR/MAPKAPK3 axis accounts for the TSPO-mediated effects on glioma cell proliferation and mobility. Together, our present study indicated that TSPO may promote the nuclear-cytoplasmic shuttling of HUR, thus increasing the mRNA stability of MAPKAPK3 and promoting the proliferation and mobility of glioma cells. The HUR/MAPKAPK3 axis may be key targets for blocking the effects of TSPO and may contribute to glioma therapy.
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http://dx.doi.org/10.1080/21655979.2022.2048992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208533PMC
March 2022

Effects of Chromium Carbide Coatings on Microstructure and Thermal Conductivity of Mg/Diamond Composites Prepared by Squeeze Casting.

Materials (Basel) 2022 Feb 9;15(4). Epub 2022 Feb 9.

Institute of Advanced Manufacturing and Modern Equipment Technology, Jiangsu University, Zhenjiang 212013, China.

Magnesium matrix composites are considered a desired solution for lightweight applications. As an attractive thermal management material, diamond particle-reinforced Mg matrix (Mg/diamond) composites generally exhibit thermal conductivities lower than expected. To exploit the potential of heat conduction, a combination of Cr coating on diamond particles and squeeze casting was used to prepare Mg/diamond (Cr) composites. The thickness of the Cr coating under different coating processes (950 °C/30 min, 950 °C/60 min, 950 °C/90 min, 1000 °C/30 min, and 1050 °C/30 min) was measured by FIB-SEM to be 1.09-2.95 μm. The thermal conductivity (TC) of the Mg/diamond composites firstly increased and then decreased, while the coefficient of thermal expansion (CTE) of Mg/diamond (Cr) composite firstly decreased and then increased with the increase in Cr coating thickness. The composite exhibited the maximum TC of 202.42 W/(m·K) with a 1.20 μm Cr coating layer, while a minimum CTE of 5.82 × 10/K was recorded with a coating thickness of 2.50 μm. The results clearly manifest the effect of Cr layer thickness on the TC and CTE of Mg/diamond composites.
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http://dx.doi.org/10.3390/ma15041284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875996PMC
February 2022

Nanostrucutured MnO-TiN nanotube arrays for advanced supercapacitor electrode material.

Sci Rep 2022 Feb 8;12(1):2088. Epub 2022 Feb 8.

Shanghai Key Laboratory of R&D for Metallic Functional Materials, Tongji University, Shanghai, 201804, People's Republic of China.

The capacitance of MnO supercapacitors (SCs) is not high as expected due to its low conductivity of MnO. The synergistic effects of MnO with high theoretical specific capacitance and TiN with high theoretical conductivity can extremely enhance the electrochemical performance of the MnO-TiN electrode material. In this work, we synthesized different nanostructured and crystalline-structured MnO modified TiN nanotube arrays electrode materials by hydrothermal method and explained the formation mechanism of different nanostructured and crystalline-structured MnO The influences of MnO nanostructures and crystalline-structures on the electrochemical performance has been contrasted and discussed. The specific capacitance of δ-MnO nanosheets-TiN nanotube arrays can reach 689.88 F g, the highest value among these samples TN-MO-SS, TN-MO-S, TN-MO-SR, TN-MO-RS, and TN-MO-R. The reason is explained based on MnO nanostructure and crystalline-structure and electron/ion transport properties. The specific capacitance retention rates are 97.2% and 82.4% of initial capacitance after 100 and 500 cycles, respectively, indicating an excellent charging-discharging cycle stability.
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http://dx.doi.org/10.1038/s41598-022-05167-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826938PMC
February 2022

Lignin-Supported Heterogeneous Photocatalyst for the Direct Generation of HO from Seawater.

J Am Chem Soc 2022 02 7;144(6):2603-2613. Epub 2022 Feb 7.

Department of Chemistry, Universiteit Antwerpen, Antwerp 2020, Belgium.

The development of smart and sustainable photocatalysts is in high priority for the synthesis of HO because the global demand for HO is sharply rising. Currently, the global market share for HO is around 4 billion US$ and is expected to grow by about 5.2 billion US$ by 2026. Traditional synthesis of HO via the anthraquinone method is associated with the generation of substantial chemical waste as well as the requirement of a high energy input. In this respect, the oxidative transformation of pure water is a sustainable solution to meet the global demand. In fact, several photocatalysts have been developed to achieve this chemistry. However, 97% of the water on our planet is seawater, and it contains 3.0-5.0% of salts. The presence of salts in water deactivates the existing photocatalysts, and therefore, the existing photocatalysts have rarely shown reactivity toward seawater. Considering this, a sustainable heterogeneous photocatalyst, derived from hydrolysis lignin, has been developed, showing an excellent reactivity toward generating HO directly from seawater under air. In fact, in the presence of this catalyst, we have been able to achieve 4085 μM of HO. Expediently, the catalyst has shown longer durability and can be recycled more than five times to generate HO from seawater. Finally, full characterizations of this smart photocatalyst and a detailed mechanism have been proposed on the basis of the experimental evidence and multiscale/level calculations.
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http://dx.doi.org/10.1021/jacs.1c10786DOI Listing
February 2022

The role of tolvaptan in managing hyponatremia in small cell lung cancer patients with SIADH: a retrospective study of 23 cases.

Authors:
Peng Ren Qiuan Yang

Transl Cancer Res 2021 Mar;10(3):1229-1237

Department of Radiation Oncology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: Existing studies have shed light on the treatment of small cell lung cancer (SCLC), but data on tolvaptan for the treatment of hyponatremia in SCLC patients remain scarce. Furthermore, the most appropriate initial dose has not been identified. This study aimed to assess the effectiveness, safety, and survival rate associated with tolvaptan in regard to controlling hyponatremia in SCLC patients with the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and to explore the appropriate initial dose.

Methods: A total of 23 SCLC patients with SIADH treated with tolvaptan were retrospectively reviewed between 2011 and 2019, and the efficacy and safety of tolvaptan were evaluated and compared between a 3.75 mg dose and doses higher than 3.75 mg. Then, the Kaplan-Meier method was used to calculate overall survival (OS) and draw survival curves.

Results: In our center, patients had a mean age of 61.7±8.2 years. The mean plasma sodium level after hypertonic saline treatment was (120.7±7.5) mmol/L. The doses of tolvaptan were distributed as follows: 3.75 mg (10 patients), 5 mg (5 patients), 7.5 mg (4 patients), and 15 mg (4 patients). After 3 days of tolvaptan treatment, the mean plasma sodium level was (136.0±4.1) mmol/L, which was significantly higher than the plasma sodium level on admission and before tolvaptan treatment (P<0.05). The mean time for tolvaptan to correct the plasma sodium level was (3.7±5.8) days, and no significant difference was found between the 3.75 mg group and the above 3.75 mg group (P>0.05). Adverse events were observed in 1 patient who presented with severe lethargy and confusion. The median OS was 14.1 months.

Conclusions: In summary, no significant difference was found between the 3.75 mg group and the above 3.75 mg group. Less than 15 mg of tolvaptan is enough to stabilize sodium levels for most patients. There is a risk of overcorrection of plasma sodium when using 15 mg of tolvaptan. Tolvaptan can quickly improve performance status and may bring survival benefits to patients. The effect of tolvaptan in patients with SCLC and SIADH should be confirmed.
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http://dx.doi.org/10.21037/tcr-20-2123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797353PMC
March 2021

Side-Chain Engineering of Conjugated Polymers for High-Performance Organic Field-Effect Transistors.

J Phys Chem Lett 2022 Feb 27;13(4):1131-1146. Epub 2022 Jan 27.

State Key Laboratory of Applied Organic Chemistry (SKLAOC), College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Past decades have witnessed the rapid development of conjugated polymers because of their promising semiconducting properties and applications in organic field-effect transistors (OFETs). Recent studies have shown that side-chain engineering of conjugated polymers is an efficient strategy to increase semiconducting performance. This Perspective focuses on the side-chain modulation of conjugated polymers and evaluating their effects on the performance of OFETs. The challenges and potential applications of functional high-performance OFETs through side-chain engineering are also discussed.
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http://dx.doi.org/10.1021/acs.jpclett.1c03909DOI Listing
February 2022

Nitric oxide impairs spatial learning and memory in a rat model of Alzheimer's disease via disturbance of glutamate response in the hippocampal dentate gyrus during spatial learning.

Behav Brain Res 2022 03 14;422:113750. Epub 2022 Jan 14.

Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, China. Electronic address:

Nitric oxide (NO)-dependent pathways may play a significant role in the decline of synaptic and cognitive functions in Alzheimer's disease (AD). However, whether NO in the hippocampal dentate gyrus (DG) is involved in the spatial learning and memory impairments of AD by affecting the glutamate (Glu) response during these processes is not well-understood. Here, we prepared an AD rat model by long-term i.p. of D-galactose into ovariectomized rats, and then the effects of L-NMMA (a NO synthase inhibitor) on Glu concentration and amplitude of field excitatory postsynaptic potential (fEPSP) were measured in the DG region during the Morris water maze (MWM) test in freely-moving rats. During the MWM test, compared with the sham group, the escape latency was increased in the place navigation trial, and the percentage of time spent in target quadrant and the number of platform crossings were decreased in the spatial probe trial, in addition, the increase of fEPSP amplitude in the DG was significantly attenuated in AD group rats. L-NMMA significantly attenuated the spatial learning and memory impairment in AD rats, and reversed the inhibitory effect of AD on increase of fEPSP amplitude in the DG during the MWM test. In sham group rats, the Glu level in the DG increased significantly during the MWM test, and this response was markedly enhanced in AD rats. Furthermore, the response of Glu in the DG during spatial learning was recovered by microinjection of L-NMMA into the DG. Our results suggest that NO in the DG impairs spatial learning and memory and related synaptic plasticity in AD rats, by disturbing the Glu response during spatial learning.
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http://dx.doi.org/10.1016/j.bbr.2022.113750DOI Listing
March 2022

Fabrication of Conductive, Adhesive, and Stretchable Agarose-Based Hydrogels for a Wearable Biosensor.

ACS Appl Bio Mater 2021 08 29;4(8):6148-6156. Epub 2021 Jun 29.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, China.

Herein, a strategy is proposed to prepare a conductive, self-adhesive, and stretchable agarose gel with the merits of distinct heat resistance, freeze resistance, and long-term moisture retention. To endow the gels with conductivity, monodisperse carbon nanotubes modified by polydopamine are introduced into the gel networks, which promote both conductivity and mechanical strength of the gels. Meanwhile, further addition of glycerol enhances excellent stretchability as well as heating/freezing tolerability and moisture retention of the gels. A wearable biosensor based on the gel is fabricated to record body motions precisely with good biocompatibility, which benefits the development of smart wearable devices.
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http://dx.doi.org/10.1021/acsabm.1c00501DOI Listing
August 2021

Microplastics boost the accumulation of tetrabromobisphenol A in a commercial clam and elevate corresponding food safety risks.

Chemosphere 2022 Apr 31;292:133499. Epub 2021 Dec 31.

College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Marine bivalve molluscs are one of the primary seafood for consumers. Inhabiting terrigenous pollutant-convergent coastal areas and feeding through seawater filtration, edible bivalves are subjected to waterborne emerging pollutants such as microplastics (MPs) and tetrabromobisphenol A (TBBPA). Nevertheless, the potential risks of consuming MP-TBBPA contaminated seafood are still largely unknown. With that, accumulation of TBBPA with and without the presence of MPs in a commercial bivalve species, blood clam (Tegillarca granosa), was determined in the present study. Meanwhile, corresponding target hazard quotients (THQs) as well as margins of exposure (MoEs) were estimated to evaluate the potential health risks for clam consumers. Furthermore, the impacts of pollutants accumulation on the detoxification process and energy supply were analysed. The data obtained demonstrated that MPs aggravate the accumulation of TBBPA in clams, leading to elevated potential food safety risks (indicated by higher THQ values and lower MoE values) for consumers. In addition, the in vivo contents of CYP1A1 and UDP-glucuronosyltransferase, the enzymatic activity of glutathione-S-transferase, and the expression levels of five detoxification-related genes were all dramatically suppressed by MP-TBBPA. Furthermore, clams exposed to MP-TBBPA had significantly lower adenosine triphosphate contents and lower pyruvate kinase and phosphofructokinase activities. These results indicated that the aggravation of TBBPA accumulation may be due to the hence disruption of detoxification process and limited energy available for detoxification.
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http://dx.doi.org/10.1016/j.chemosphere.2021.133499DOI Listing
April 2022

[Bone tunnel positions in anterior cruciate ligament reconstruction evaluated by three-dimensional CT reconstruction based on Mimics software:modified transtibial versus anteromedial portal technique].

Zhongguo Gu Shang 2021 Dec;34(12):1126-31

Department of Orthopaedics, the Second Affiliated Hospital of Jiaxing College, Jiaxing 314000, Zhejing, China.

Objective: To compare the femoral and tibial tunnel positions of anterior cruciate ligament reconstruction using the modified transtibial (MTT) technique and anteromedial (AM) portal technique.

Methods: Between January 2017 and September 2020, 78 patients with anterior cruciate ligament rupture underwent single-bundle reconstruction with the modified transtibial technique in 39 cases (group MTT) and through anteromedial approach in 39 cases (group AM). There were 25 males and 14 females in group MTT, with an average age of (37.0±2.3) years old; 27 males and 12 females in group AM, with an average age of (37.5±2.2) years old. CT scan of the affected knee was conducted one week after the surgery to measure and compare the femoral tunnels positioning (Fx, Fy), tibial tunnels positioning in the frontal plane(Tx1), tibial tunnels positioning in the sagittal plane (Ty1), and tibial tunnels positioning in the axial plane (Tx2, Ty2) in patients undergoing anterior cruciate ligament reconstruction through Mimics software.

Results: Three-dimensional CT reconstruction after the surgery showed that the average Fx and Fy were(25.2±2.1)% and (34.9±3.0)% respectively and the Tx1 and Ty1 were (45.5±3.3)% and (44.7± 3.0)% respectively, while the Tx2 and Ty2 were (47.0±3.0)% and (39.9±4.2)% respectively in group MTT. In group AM, the average Fx and Fy were (26.0±2.0)% and (36.1±3.9)% respectively and the Tx1 and Ty1 were (46.5±3.1)% and (45.6± 3.1)% respectively, while the Tx2 and Ty2 were (47.4±2.5)% and (39.6±3.9)% respectively. There were no statistically significant differences in the femoral and tibial tunnels between the two groups (>0.05). Patients in both two groups obtained anatomic anterior cruciate ligament reconstruction.

Conclusion: Both the MTT and AM technique can achieve good anatomical positioning of the femoral and tibial tunnels, without significant differences in the positioning of the bone tunnels.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.12.007DOI Listing
December 2021

The correspondence between morphometric MRI and metabolic profile in Rasmussen's encephalitis.

Neuroimage Clin 2022 20;33:102918. Epub 2021 Dec 20.

Department of Neurosurgery, SanBo Brain Hospital, Capital Medical University, Beijing 100093, China; Key Laboratory of Epilepsy, Beijing 100093, China; Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing 100093, China. Electronic address:

Volumetric magnetic resonance imaging (MRI) atrophy is a hallmark of Rasmussen's encephalitis (RE). Here, we aim to investigate voxel-wise gray matter (GM) atrophy in RE, and its associations with glucose hypometabolism and neurotransmitter distribution utilizing MRI and PET data. In this study, fifteen RE patients and fourteen MRI normal subjects were included in this study. Voxel-wise GM volume and glucose metabolic uptake were evaluated using structural MRI and FDG-PET images, respectively. Spatial Spearman's correlation was performed between GM atrophy of RE with FDG uptake alterations, and neurotransmitter distributions provided in the JuSpace toolbox. Compared with the control group, RE patients displayed extensive GM volume loss not only in the ipsilateral hemisphere, but also in the frontal lobe, basal ganglia, and cerebellum in the contralateral hemisphere. Within the RE group, the insular and temporal cortices exhibited significantly more GM atrophy on the ipsilesional than the contralesional side. FDG-PET data revealed significant hypometabolism in areas surrounding the insular cortices in the ipsilesional hemisphere. RE-related GM volumetric atrophy was spatially correlated with hypomebolism in FDG uptake, and with spatial distribution of the dopaminergic and serotonergic neurotransmitter systems. The spatial concordance of morphological changes with metabolic abnormalities suggest FDG-PET offers potential value for RE diagnosis. The GM alterations associated with neurotransmitter distribution map could provide novel insight in understanding the neuropathological mechanisms and clinical feature of RE.
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http://dx.doi.org/10.1016/j.nicl.2021.102918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713113PMC
March 2022

Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability.

Pharmacol Res 2022 01 9;175:106020. Epub 2021 Dec 9.

Department of microbiology and immunology, School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi, China. Electronic address:

Obesity associated with low-grade chronic inflammation and intestinal dysbiosis is considered as a worldwide public health crisis. In the meanwhile, different probiotics have demonstrated beneficial effects on this condition, thus increasing the interest in the development of probiotic treatments. In this context, the aim of this study is to investigate the anti-obesity effects of potential probiotic Lactobacillus acidophilus isolated from the porcine gut. Then, it is found that L. acidophilus reduces body weight, fat mass, inflammation and insulin resistance in mice fed with a high-fat diet (HFD), accompanied by activation in brown adipose tissue (BAT) as well as improvements of energy, glucose and lipid metabolism. Besides, our data indicate that L. acidophilus not only reverses HFD-induced gut dysbiosis, as indicated by the decreased Firmicutes-to-Bacteroidetes ratios and endotoxin bearing Gram-negative bacteria levels, but also maintains intestinal barrier integrity, reduces metabolic endotoxemia, and inhibits the TLR4 / NF- κB signaling pathway. In addition, the results of microbiome phenotype prediction by BugBase and bacterial functional potential prediction using PICRUSt show that L. acidophilus treatment improves the gut microbiota functions involving metabolism, immune response, and pathopoiesia. Furthermore, the anti-obesity effect is transmissible via horizontal faeces transfer from L. acidophilus-treated mice to HFD-fed mice. According to our data, it is seen that L. acidophilus could be a good candidate for probiotic of ameliorating obesity and associated diseases such as hyperlipidemia, nonalcoholic fatty liver diseases, and insulin resistance through its anti-inflammatory properties and alleviation of endothelial dysfunction and gut dysbiosis.
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http://dx.doi.org/10.1016/j.phrs.2021.106020DOI Listing
January 2022

Selective Separation of Am(III)/Eu(III) by the QL-DAPhen Ligand under High Acidity: Extraction, Spectroscopy, and Theoretical Calculations.

Inorg Chem 2021 Dec 3;60(24):19110-19119. Epub 2021 Dec 3.

Laboratory of Nuclear Energy Chemistry, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P.R. China.

Although 1,10-phenanthroline-based ligands have recently shown vast opportunities for the separation of trivalent actinides (Ans(III)) from lanthanides (Lns(III)), the optimization and design of the extractant structure based on the phenanthroline framework remain hotspots for further improving the separation. Following the strategy of hard and soft donor atom combination, for the first time, the quinoline group was attached to the 1,10-phenanthroline skeleton, giving a lipophilic ligand, 2,9-diacyl-bis((3,4-dihydroquinoline-1((2H)-yl)-1),10-phenanthroline (QL-DAPhen)), for Am(III)/Eu(III) separation. In the presence of sodium nitrate, the ligand can effectively extract Am(III) over Eu(III) in HNO solution, with the separation factor () ranging from 29 to 44. The coordination chemistry of Eu(III) with QL-DAPhen was investigated by slope analysis, NMR titration, UV-vis titration, Fourier transform infrared spectroscopy, electrospray ionization-mass spectrometry, and theoretical calculations. The experimental results unanimously confirm that the ligand forms both 1:1 and 1:2 complexes with Eu(III), and the stability constants (log ) of each of the two complexes were obtained. Density functional theory calculations show that the Am-N bonds have more covalent characteristics than the Eu-N bonds in the complexes, which reveals the reason why the ligand preferentially bonds with Am(III). Meanwhile, the thermodynamic analysis reveals that the 1:1 complex is more thermodynamically stable than the 1:2 complex. The findings of this work have laid a solid theoretical foundation for the application of phenanthroline-based ligands in the separation of An(III) from practical systems.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02916DOI Listing
December 2021

Analysis of ECG Signals by Dynamic Mode Decomposition.

IEEE J Biomed Health Inform 2022 05 5;26(5):2124-2135. Epub 2022 May 5.

Objective: Based on cybernetics, a large system can be divided into subsystems, and the stability of each can determine the overall properties of the system. However, this stability analysis perspective has not yet been employed in electrocardiogram (ECG) signals. This is the first study to attempt to evaluate whether the stability of decomposed ECG subsystems can be analyzed in order to effectively investigate the overall performance of ECG signals, and aid in disease diagnosis.

Methods: We used seven different cardiac pathologies (myocardial infarction, cardiomyopathy, bundle branch block, dysrhythmia, hypertrophy, myocarditis, and valvular heart disease) to illustrate our method. Dynamic mode decomposition (DMD) was first used to decompose ECG signals into dynamic modes (DMs) which can be regarded as ECG subsystems. Then, the features related to the DMs stabilities were extracted, and nine common classifiers were implemented for classification of these pathologies.

Results: Most features were significant for differentiating the above-mentioned groups (p value<0.05 after Bonferroni correction). In addition, our method outperformed all existing methods for cardiac pathology classification.

Conclusion: We have provided a new spatial and temporal decomposition method, namely DMD, to study ECG signals.

Significance: Our method can reveal new cardiac mechanisms, which can contribute to the comprehensive understanding of its underlying mechanisms and disease diagnosis, and thus, can be widely used for ECG signal analysis in the future.
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http://dx.doi.org/10.1109/JBHI.2021.3130275DOI Listing
May 2022
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