Publications by authors named "Peng Liu"

3,720 Publications

  • Page 1 of 1

Chain-shattering polymeric sulfur dioxide prodrug micelles for redox-triggered gas therapy of osteosarcoma.

J Mater Chem B 2022 Jun 28. Epub 2022 Jun 28.

National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, 518036, P. R. China.

Sulfur dioxide (SO) based gas therapy has received great attention recently. Nevertheless, it is still a challenge to fabricate a SO delivery system to achieve effective delivery and on-demand stimuli triggered release at tumor sites. Herein, a chain-shattering polymeric SO prodrug micelle system was fabricated for effective SO based gas therapy. First, an amphiphilic polymer (mPEG-P(HDI-DN)) was prepared by polycondensation of poly(ethylene glycol) methyl ether, hexamethylene diisocyanate and monomer containing SO. mPEG-P(HDI-DN) can self-assemble into spherical micelles with a diameter of around 50-90 nm. Triggered release of SO from micelles can be achieved in the presence of GSH with the degradation of mPEG-P(HDI-DN) into small molecules. The experiment proved that mPEG-P(HDI-DN) micelles can enter into osteosarcoma cells and inhibit the growth of osteosarcoma cells by increasing the ROS level in cells. The experiments demonstrate that mPEG-P(HDI-DN) micelles can inhibit the growth of osteosarcoma effectively without obvious tissue toxicity. These results indicate that this chain-shattering polymeric SO prodrug micelle system is a promising candidate for effective SO based gas therapy.
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http://dx.doi.org/10.1039/d2tb00287fDOI Listing
June 2022

Nanoencapsulation of chitooligosaccharides enhances its oral bioavailability and anti-liver fibrotic effects.

Food Res Int 2022 Jul 8;157:111471. Epub 2022 Jun 8.

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address:

Chitooligosaccharides (COS) significantly attenuates liver dysfunction. However, the conundrum of the oral bioavailability of COS limits their pharmacological effects. Therefore, a strategy of nanoencapsulation was employed to enhance oral bioavailability and tissue-targeted distribution of COS. In this study, nanospheres loaded with COS (CANs) were prepared, their bioavailability, biodistribution, transport mechanism and anti-liver fibrosis effects were explored. Nanoencapsulation improved the oral bioavailability of various COS monomers through microfold cell-mediated absorption route in an indiscriminate manner. CANs were more favorably enriched and protractedly accumulated in the liver. In a liver fibrosis model, CANs ameliorated the pathological state and extracellular matrix deposition. The alleviation of liver fibrosis for COS could be attributed to the inhibition of liver cell apoptosis and liver sinusoidal endothelial cell (LSEC) capillarization. Consequently, this study highlights the improved oral bioavailability of COS and proposes a novel mechanism of COS, for better understanding its hepatoprotective effect.
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http://dx.doi.org/10.1016/j.foodres.2022.111471DOI Listing
July 2022

Community composition, bacterial symbionts, antibacterial and antioxidant activities of honeybee-associated fungi.

BMC Microbiol 2022 Jun 27;22(1):168. Epub 2022 Jun 27.

School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.

Background: Fungi associated with insects represent one potentially rich source for the discovery of novel metabolites. However, a comprehensive understanding of the fungal communities of Apis mellifera ligustica remains elusive.

Results: Here, we investigated the phylogenetic diversity and community composition of honeybee-associated fungi using combination of culture-dependent and culture-independent approaches. A total of forty-five fungi were isolated and purified from the Apis mellifera ligustica, royal jelly, and honeycomb, which belonged to four classes and eleven different genera. Furthermore, 28 bacterial 16S rRNA gene sequences were obtained by PCR from the fungal metagenome. High-throughput sequencing analyses revealed that the fungal communities were more diverse, a total of 62 fungal genera were detected in the honeybee gut by culture-independent method, whereas only 4 genera were isolated by culture-dependent method. Similarly, 247 fungal genera were detected in the honeycomb, whereas only 4 genera were isolated. In addition, we assessed the antibacterial and antioxidant activities of fungal isolates. Most fungal crude extracts obtained from the cultivation supernatant exhibited antioxidant activities. Only two fungal crude extracts displayed moderate activity against Escherichia coli and Staphylococcus aureus. Chemical analysis of Chaetomium subaffine MFFC22 led to the discovery of three known compounds, including cochliodinol (1), emodin (2), chrysophanol (3). Among them, cochliodinol (1) showed intense DPPH radical scavenging activity with the 50% inhibitory concentration (IC) of 3.06 μg/mL, which was comparable to that of the positive ascorbic acid (IC = 2.25 μg/mL). Compound 2 displayed weak inhibitory activities against Micrococcus tetragenus and S. aureus.

Conclusions: This research provided a fundamental clue for the complex interactions among honeybees, fungi, bacterial symbionts, and the effects on the honeybee. Furthermore, the diversity of honeybee-associated fungi had great potential in finding the resource of new species and antioxidants.
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http://dx.doi.org/10.1186/s12866-022-02580-4DOI Listing
June 2022

A Novel Pathway for Porcine Epidemic Diarrhea Virus Transmission from Sows to Neonatal Piglets Mediated by Colostrum.

J Virol 2022 Jun 27:e0047722. Epub 2022 Jun 27.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.

The mechanisms of colostrum-mediated virus transmission are difficult to elucidate because of the absence of experimental animal models and the difficulties in tissue sample collection from mothers in the peripartum period. Porcine epidemic diarrhea virus (PEDV) is a reemerging enteropathogenic coronavirus that has catastrophic impacts on the global pig industry. PEDV primarily infects neonatal piglets by multiple routes, especially 1- to 2-day-old neonatal piglets. Here, our epidemiological investigation and animal challenge experiments revealed that PEDV could be vertically transmitted from sows to neonatal piglets via colostrum, and CD3 T cells in the colostrum play an important role in this process. The results showed that PEDV colonizing the intestinal epithelial cells (IECs) of orally immunized infected sows could be transferred to CD3 T cells located just beneath the IECs. Next, PEDV-carrying CD3 T cells, with the expression of integrin α4β7 and CCR10, migrate from the intestine to the mammary gland through blood circulation. Arriving in the mammary gland, PEDV-carrying CD3 T cells could be transported across mammary epithelial cells (MECs) into the lumen (colostrum), as illustrated by an autotransfusion assay and an MECs/T coculture system. The PEDV-carrying CD3 T cells in colostrum could be interspersed between IECs of neonatal piglets, causing intestinal infection via cell-to-cell contact. Our study demonstrates for the first time that colostrum-derived CD3 T cells comprise a potential route for the vertical transmission of PEDV. The colostrum represents an important infection route for many viruses. Here, we demonstrate the vertical transmission of porcine epidemic diarrhea virus (PEDV) from sows to neonatal piglets via colostrum. PEDV colonizing the intestinal epithelial cells could transfer the virus to CD3 T cells located in the sow intestine. The PEDV-carrying CD3 T cells in the sow intestine, with the expression of integrin α4β7 and CCR10, arrive at the mammary gland through blood circulation and are transported across mammary epithelial cells into the lumen, finally leading to intestinal infection via cell-to-cell contact in neonatal piglets. Our study not only demonstrates an alternative route of PEDV infection but also provides an animal model of vertical transmission of human infectious disease.
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http://dx.doi.org/10.1128/jvi.00477-22DOI Listing
June 2022

Microglia Regulate Blood-Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory Demyelination.

Adv Sci (Weinh) 2022 Jun 27:e2105442. Epub 2022 Jun 27.

Institute of Neuroscience, Key Laboratory of Molecular Neurobiology of Ministry of Education and the Collaborative Innovation Center for Brain Science, SMMU, Shanghai, 200433, China.

Blood-brain barrier (BBB) impairment is an early prevalent feature of multiple sclerosis (MS), and remains vital for MS progression. Microglial activation precedes BBB disruption and cellular infiltrates in the brain of MS patients. However, little is known about the function of microglia in BBB impairment. Here, microglia acts as an important modulator of BBB integrity in inflammatory demyelination. Microglial depletion profoundly ameliorates BBB impairment in experimental autoimmune encephalomyelitis (EAE). Specifically, miR-126a-5p in microglia is positively correlated with BBB integrity in four types of MS plaques. Mechanistically, microglial deletion of miR-126a-5p exacerbates BBB leakage and EAE severity. The protective effect of miR-126a-5p is mimicked and restored by specific inhibition of MMP9 in microglia. Importantly, Auranofin, an FDA-approved drug, is identified to protect BBB integrity and mitigate EAE progression via a microglial miR-126a-5p dependent mechanism. Taken together, microglia can be manipulated to protect BBB integrity and ameliorate inflammatory demyelination. Targeting microglia to regulate BBB permeability merits consideration in therapeutic interventions in MS.
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http://dx.doi.org/10.1002/advs.202105442DOI Listing
June 2022

Distribution Law of Corrosion Products in a Marine Chloride Environment.

Materials (Basel) 2022 Jun 19;15(12). Epub 2022 Jun 19.

National Engineering Laboratory for High Speed Railway Construction, 22 Shaoshan Road, Changsha 410004, China.

Steel corrosion is the main cause of reinforced concrete cracking. Conventionally, concrete is considered to crack when the circumferential tensile stress reaches the tensile strength of the concrete. However, few analyses have considered the fracture criteria of the internal cross-section of concrete. Based on the von Mises distribution of angle probabilities, this paper proposes a new probability distribution function for investigating the distribution law of corrosion products. The cracking process of experimental samples was numerically analyzed, and the results were consistent with those of the theoretical model. The effect of the dry-wet cycle ratio on the corrosion products was preliminarily investigated by microscopic observation of the reinforced concrete under different dry-wet cycle corrosion environments.
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http://dx.doi.org/10.3390/ma15124339DOI Listing
June 2022

Suilyin Disrupts the Blood-Brain Barrier by Activating Group III Secretory Phospholipase A2.

Life (Basel) 2022 Jun 20;12(6). Epub 2022 Jun 20.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.

Serious diseases caused by serotype 2 ( 2) include septicaemia and meningitis, which are associated with high morbidity and mortality. Proliferation in the blood can result in a breach of the blood-brain barrier (BBB) and provide entry into the cerebrospinal fluid (CSF), where bacteria cause inflammation of the meningeal membranes resulting in meningitis. The molecular mechanisms of how this pathogen crosses the BBB remain unclear. Suilysin (SLY) has been identified as an important secreted virulence factor of 2 and may play a vital role in provoking meningitis. In this investigation, we demonstrate that SLY can increase the paracellular permeability of BBB, both in vivo and in vitro, via the activation of group III secretory phospholipase A2 (PLA2G3). Our results indicate that at lower, sublytic concentrations, the toxin can stimulate cerebral microvascular endothelial cells to release TNF-α, thereby inducing high level expressions of PLA2G3. Abnormal elevations of PLA2G3 might further injure tissues through direct cytolytic effectors or other responses.
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http://dx.doi.org/10.3390/life12060919DOI Listing
June 2022

Collaborative Action of Microglia and Astrocytes Mediates Neutrophil Recruitment to the CNS to Defend against K1 Infection.

Int J Mol Sci 2022 Jun 11;23(12). Epub 2022 Jun 11.

The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300457, China.

K1 is a leading cause of neonatal bacterial meningitis. Recruitment of neutrophils to the central nervous system (CNS) via local immune response plays a critical role in defense against K1 infection; however, the mechanism underlying this recruitment remains unclear. In this study, we report that microglia and astrocytes are activated in response to stimulation by K1 and/or K1-derived outer membrane vesicles (OMVs) and work collaboratively to drive neutrophil recruitment to the CNS. Microglial activation results in the release of the pro-inflammatory cytokine TNF-α, which activates astrocytes, resulting in the production of CXCL1, a chemokine critical for recruiting neutrophils. Mice lacking either microglia or TNF-α exhibit impaired production of CXCL1, impaired neutrophil recruitment, and an increased CNS bacterial burden. C-X-C chemokine receptor 2 (CXCR2)-expressing neutrophils primarily respond to CXCL1 released by astrocytes. This study provides further insights into how immune responses drive neutrophil recruitment to the brain to combat K1 infection. In addition, we show that direct recognition of K1 by microglia is prevented by the K1 capsule. This study also reveals that OMVs are sufficient to induce microglial activation.
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http://dx.doi.org/10.3390/ijms23126540DOI Listing
June 2022

NeDSeM: Neutrosophy Domain-Based Segmentation Method for Malignant Melanoma Images.

Entropy (Basel) 2022 Jun 2;24(6). Epub 2022 Jun 2.

Department of Computer Science and Technology, Harbin Engineering University, Harbin 150001, China.

Skin lesion segmentation is the first and indispensable step of malignant melanoma recognition and diagnosis. At present, most of the existing skin lesions segmentation techniques often used traditional methods like optimum thresholding, etc., and deep learning methods like U-net, etc. However, the edges of skin lesions in malignant melanoma images are gradually changed in color, and this change is nonlinear. The existing methods can not effectively distinguish banded edges between lesion areas and healthy skin areas well. Aiming at the uncertainty and fuzziness of banded edges, the neutrosophic set theory is used in this paper which is better than fuzzy theory to deal with banded edge segmentation. Therefore, we proposed a neutrosophy domain-based segmentation method that contains six steps. Firstly, an image is converted into three channels and the pixel matrix of each channel is obtained. Secondly, the pixel matrixes are converted into Neutrosophic Set domain by using the neutrosophic set conversion method to express the uncertainty and fuzziness of banded edges of malignant melanoma images. Thirdly, a new Neutrosophic Entropy model is proposed to combine the three memberships according to some rules by using the transformations in the neutrosophic space to comprehensively express three memberships and highlight the banded edges of the images. Fourthly, the feature augment method is established by the difference of three components. Fifthly, the dilation is used on the neutrosophic entropy matrixes to fill in the noise region. Finally, the image that is represented by transformed matrix is segmented by the Hierarchical Gaussian Mixture Model clustering method to obtain the banded edge of the image. Qualitative and quantitative experiments are performed on malignant melanoma image dataset to evaluate the performance of the NeDSeM method. Compared with some state-of-the-art methods, our method has achieved good results in terms of performance and accuracy.
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http://dx.doi.org/10.3390/e24060783DOI Listing
June 2022

The formation of specific bacterial communities contributes to the enrichment of antibiotic resistance genes in the soil plastisphere.

J Hazard Mater 2022 08 3;436:129247. Epub 2022 Jun 3.

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.

Soil serves as a major reservoir of both antibiotic resistance genes (ARGs) and microplastics. However, the characteristics of the antibiotic resistome in the soil plastisphere remain largely unknown. In this study, we used metagenomic approaches to reveal the changing patterns of ARGs and the bacterial community and their associations in response to three types of microplastics (light density polyethylene, LDPE; polypropylene, PP; polystyrene, PS) using particles 550 µm or 75 µm in diameter. The total ARG abundances significantly increased in the plastisphere and varied across plastic types. The LDPE plastisphere had the highest ARG total abundance and lowest Shannon diversity index, indicating that this plastic had the most severe negative impact on soil bacterial diversity. The PP plastisphere contained higher relative abundances of the pathogenic bacteria Acinetobacter johnsonii and Escherichia coli, demonstrating the higher pathogenic risk of the microbial communities enriched in the plastisphere. Specifically, multidrug resistance genes (ceoB and MuxB) co-existed with more than four microbial taxa, increasing the potential risk of ARG spread in pathogenic bacteria. These findings implied that the plastisphere acts as a hotspot for acquiring and spreading antibiotic resistance and may have long-term negative effects on the soil ecosystem and human health.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129247DOI Listing
August 2022

Electrospun UiO-66-F/polyacrylonitrile nanofibers for efficient extraction of perfluoroalkyl and polyfluoroalkyl substances in environmental media.

J Hazard Mater 2022 05 16;430:128494. Epub 2022 Feb 16.

Department of Preventive Medicine, School of Public Health, Wuhan University, Wuhan 430071, PR China. Electronic address:

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a family of emerging contaminants which are widely present in environment. In this work, novel UiO-66-F/polyacrylonitrile (UiO-66-F/PAN) hybrid nanofibers were firstly prepared via blend electrospinning or in-situ growth method for the pipette-tip solid phase extraction of PFASs. Characterizations demonstrate the as-synthesized UiO-66-F/PAN nanofibers have good chemical and thermal stability, possess large surface area (248 m²/g) and mesoporous framework structure. Several extraction factors including the amount of adsorbent, pH and ionic strength of sample solution, extraction time and eluent were investigated and the optimum conditions are 20 mg of the selected sorbent, adjusting to pH 5 and adding 4% w/v NaCl to sample solution, extraction for 12 min (3 min × 4). The good adsorption affinity of UiO-66-F/PAN for PFASs can be attributed to the extensive adsorption sites and multiple interactions including hydrophobic interaction, hydrogen bonding and F-F interaction. Low limit of detection (0.008-0.076 µg/L), limit of quantification (0.010-0.163 µg/L) and recoveries (70.84-113.57%) for 9 PFASs with relative standard deviations < 15% were achieved. When applied in the analysis of target PFASs in lake water, tap water, beverage, and shrimp muscle samples, this method could achieve robust and accurate results with sufficient sensitivity for nine PFASs.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128494DOI Listing
May 2022

Gray matter microstructural alterations in manganese-exposed welders: a preliminary neuroimaging study.

Eur Radiol 2022 Jun 23. Epub 2022 Jun 23.

Department of Radiology, The Affiliated Xi'an Central Hospital of Xi'an Jiaotong University, Xi'an, China.

Objectives: Chronic occupational manganese (Mn) exposure is characterized by motor and cognitive dysfunction. This study aimed to investigate structural abnormalities in Mn-exposed welders compared to healthy controls (HCs).

Methods: Thirty-five HCs and forty Mn-exposed welders underwent magnetic resonance imaging (MRI) scans in this study. Based on T1-weighted MRI, the voxel-based morphometry (VBM), structural covariance, and receiver operating characteristic (ROC) curve were applied to examine whole-brain structural changes in Mn-exposed welders.

Results: Compared to HCs, Mn-exposed welders had altered gray matter volume (GMV) mainly in the medial prefrontal cortex, lentiform nucleus, hippocampus, and parahippocampus. ROC analysis indicated the potential highest classification power of the hippocampus/parahippocampus. Moreover, distinct structural covariance patterns in the two groups were associated with regions, mainly including the thalamus, insula, amygdala, sensorimotor area, and middle temporal gyrus. No significant relationships were found between the findings and clinical characteristics.

Conclusions: Our findings showed Mn-exposed welders had changed GMV and structural covariance patterns in some regions, which implicated in motivative response, cognitive control, and emotional regulation. These results might provide preliminary evidence for understanding the pathophysiology of Mn overexposure.

Key Points: • Chronic Mn exposure might be related to abnormal brain structural neural mechanisms. • Mn-exposed welders had morphological changes in brain regions implicated in emotional modulation, cognitive control, and motor-related response. • Altered gray matter volume in the hippocampus/parahippocampus and putamen might serve as potential biomarkers for Mn overexposure.
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http://dx.doi.org/10.1007/s00330-022-08908-yDOI Listing
June 2022

Predicting the Risk of Dental Implant Loss Using Deep Learning.

J Clin Periodontol 2022 Jun 23. Epub 2022 Jun 23.

Stomatological Hospital of Chongqing Medical University, Chongqing, P.R China.

Aim: To investigate the feasibility of predicting dental implant loss risk with deep learning (DL) based on preoperative cone-beam computed tomography.

Materials And Methods: Six hundred and three patients who underwent implant surgery (279 high-risk patients who did and 324 low-risk patients who did not experience implant loss within 5 years) from January 2012 to January 2020 were enrolled. Three models, a logistic regression clinical model (CM) based on clinical features, a DL model based on radiography features, and an integrated model (IM) developed by combining CM with DL, were developed to predict the 5-year implant loss risk. The area under the receiver operating characteristic curve (AUC) was used to evaluate the model performance. Time to implant loss was considered for both groups, and Kaplan-Meier curves were created and compared by the log-rank test.

Results: The IM exhibited the best performance in predicting implant loss risk [AUC = 0.90, 95% confidence interval (CI) 0.84-0.95], followed by the DL model (AUC = 0.87, 95% CI 0.80-0.92) and the CM (AUC = 0.72, 95% CI 0.63-0.79).

Conclusion: Our study offers preliminary evidence that both the DL model and IM performed well in predicting implant fate within 5 years and thus may greatly facilitate implant practitioners in assessing preoperative risks.
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http://dx.doi.org/10.1111/jcpe.13689DOI Listing
June 2022

C2-ketonylation of carbohydrates excited-state palladium-catalyzed 1,2-spin-center shift.

Chem Sci 2022 Jun 11;13(21):6276-6282. Epub 2022 May 11.

Department of Chemistry, Institute of Chemical Biology and Drug Discovery, The State University of New York at Stony Brook Stony Brook New York 11794 USA

C2-ketonyl-2-deoxysugars, sugars with the C2-hydroxyl group replaced by a ketone side chain, are important carbohydrate mimetics in glycobiology and drug discovery studies; however, their preparation remains a vital challenge in organic synthesis. Here we report the first direct strategy to synthesize this class of glycomimetics from readily available 1-bromosugars and silyl enol ethers an excited-state palladium-catalyzed 1,2-spin-center shift (SCS) process. This step-economic reaction features broad substrate scope, has a high functional group tolerance, and can be used in late-stage functionalization of natural product- and drug-glycoconjugates. Preliminary experimental and computational mechanistic studies suggested a non-chain radical mechanism involving photoexcited palladium species, a 1,2-SCS process, and a radical Mizoroki-Heck reaction.
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http://dx.doi.org/10.1039/d2sc01042aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159084PMC
June 2022

The role of m6A in osteoporosis and in the differentiation of mesenchymal stem cells into osteoblasts and adipocytes.

Curr Stem Cell Res Ther 2022 Jun 21. Epub 2022 Jun 21.

Department of Bone & Joint Surgery, cShenzhen, PR China, 518036.

Osteoporosis is a systemic disease in which bone mass decreases, leading to increase risk of bone fragility and fracture. The occurrence of osteoporosis is believed to be related to the disruption of the differentiation of mesenchymal stem cells to osteoblasts and adipocytes. N6-adenylate methylation (m6A) modification is the most common type of chemical RNA modification and refers to a methylation modification formed by the nitrogen atom at position 6 of adenine (A), which is catalyzed by methyltransferase. The main roles of m6A are the post-transcriptional level regulation of the stability, localization, transportation, splicing, and translation of RNA; these are key elements of various biological activities, including osteoporosis and the differentiation of mesenchymal stem cells into osteoblasts and adipocytes. The main focus of this review is the role of m6A in these two biological processes.
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http://dx.doi.org/10.2174/1574888X17666220621155341DOI Listing
June 2022

Exponential Function Computation Based on DNA Strand Displacement Circuits.

IEEE Trans Biomed Circuits Syst 2022 Jun 21;PP. Epub 2022 Jun 21.

Due to its high programmability and storage, DNA circuits have been widely used in biological computing. In this paper, the addition, subtraction, multiplication, division, n-order and 1/n-order gates are built through DNA strand displacement reactions. The chemical reaction networks of the exponential function are established by using the six DNA analog computation gates. The integrated DNA strand displacement circuits are built through the chemical reaction networks. The exponential function polynomials can be computed through the integrated DNA strand displacement circuits. Finally, through visual DSD software verification, this design can realise the computation of exponential function polynomials, which provides a reference for solving exponential function equations and neural network computations in the future.
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http://dx.doi.org/10.1109/TBCAS.2022.3184760DOI Listing
June 2022

Effect of iron substitution in cryptomelane on the heterogeneous reaction with isoprene.

J Hazard Mater 2022 Jun 6;437:129293. Epub 2022 Jun 6.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China; National Engineering Laboratory for VOCs Pollution Control Technology and Equipment, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China. Electronic address:

Biogenic isoprene is an important pollutant for regional air quality. Being ubiquitously distributed on the earth surface, manganese (hydr)oxides should play a vital role in the transformation of isoprene. Cryptomelane is a typical manganese oxide with isomorphous substitution of Fe for Mn, but less attention has been paid to its heterogeneous reaction with isoprene. When Fe replaces Mn, K is depleted and Mn is oxidized to Mn. In contrast, oxygen vacancies are formed when Fe substitutes Mn. Fe substitution creates weak crystallites and abundant mesopores, resulting in the increase of isoprene adsorption. As found by theoretical calculations, the Mn-O bonds at the cross sections of the tunnels is more active than that on the outer wall of the tunnels. After the adsorption of isoprene, bridging carboxylate species and hydrogen-bonding water are produced and the surface octahedra are distorted, i.e., MnO → MnO. As the heat facilitates the breakage of Mn-O, the increase of environmental temperature enhances the oxidation of isoprene. The above findings shed light on the effect of Fe substitution in cryptomelane to enhance the oxidation of isoprene, and illustrates that heterogeneous reaction with isoprene impairs the transformation of other environmental substances on cryptomelane.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129293DOI Listing
June 2022

Integrated Microbiome and Host Transcriptome Profiles Link Parkinson's Disease to Genus: Evidence From Feces, Blood, and Brain.

Front Microbiol 2022 26;13:875101. Epub 2022 May 26.

Department of Geriatric Neurology, Shaanxi Provincial People's Hospital, Xi'an, China.

A link between the gut microbiome and Parkinson's disease (PD) has been intensively studied, and more than 100 differential genera were identified across the studies. However, the predominant genera contributing to PD remain poorly understood. Inspired by recent advances showing microbiota distribution in the blood and brain, we, here, comprehensively investigated currently available fecal microbiome data (1,914 samples) to identify significantly altered genera, which were further validated by comparison to the results from microbiome analysis of blood (85 samples) and brain (268 samples). Our data showed that the composition of fecal microbiota was different from that of blood and brain. We found that was the unique genus consistently depleted across feces, blood, and brain samples of PD patients ( < 0.05), despite using rigorous criteria to remove contaminants. Moreover, enrichment analyses revealed that host genes correlated with genus abundance were mainly involved in mitochondrial function and energy metabolism, and mapped to neurodegenerative diseases (NDDs) and metabolic diseases. A random forest classifier constructed with fecal microbiota data demonstrated that genus was an important feature contributing to discriminating PD patients from controls [receiver operating characteristic (ROC)-area under curve (AUC) = 0.704, precision-recall curve (PRC)-AUC = 0.787]. Through the integration of microbiome and transcriptome, our study depicted microbial profiles in the feces, blood, and brain of PD patients, and identified genus as a potential genus linked to PD. Further studies are greatly encouraged to determine the role of genus in the pathogenesis of PD.
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http://dx.doi.org/10.3389/fmicb.2022.875101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204254PMC
May 2022

An Eco-Friendly Conversion of Aquaculture Suspended Solid Wastes Into High-Quality Fish Food by Improving Poly-β-Hydroxybutyrate Production.

Front Physiol 2022 26;13:797625. Epub 2022 May 26.

Yancheng Institute of Technology, Yancheng, China.

The aquaculture industry is vital in providing a valuable protein food source for humans, but generates a huge amount of solid and dissolved wastes that pose great risks to the environment and aquaculture sustainability. Suspended solids (in short SS), one of the aquaculture wastes, are very difficult to be treated due to their high organic contents. The bioconversion from wastewater, food effluents, and activated sludge into poly-β-hydroxybutyrate (PHB) is a sustainable alternative to generate an additional income and could be highly attractive to the agricultural and environmental management firms. However, little is known about its potential application in aquaculture wastes. In the present study, we first determined that 7.2% of SS was PHB. Then, the production of PHB was increased two-fold by the optimal fermentation conditions of wheat bran and microbial cocktails at a C/N ratio of 12. Also, the PHB-enriched SS showed a higher total ammonia nitrogen removal rate. Importantly, we further demonstrated that the PHB-enriched SS as a feed could promote fish growth and up-regulate the expression of the immune-related genes. Our study developed an eco-friendly and simple approach to transforming problematic SS wastes into PHB-enriched high-quality food for omnivorous fish, which will increase the usage efficiency of SS and provide a cheaper diet for aquatic animals.
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http://dx.doi.org/10.3389/fphys.2022.797625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205610PMC
May 2022

Cerebellar Continuous Theta Burst Stimulation for Aphasia Rehabilitation: Study Protocol for a Randomized Controlled Trial.

Front Aging Neurosci 2022 2;14:909733. Epub 2022 Jun 2.

Department of Neurorehabilitation, Wuxi Tongren Rehabilitation Hospital, Wuxi, China.

Background: Language recovery is limited in moderate to severe post-stroke aphasia patients. Repetitive transcranial magnetic stimulation (rTMS) has emerged as a promising tool in improving language dysfunctions caused by post-stroke aphasia, but the treatment outcome is as yet mixed. Considerable evidence has demonstrated the essential involvement of the cerebellum in a variety of language functions, suggesting that it may be a potential stimulation target of TMS for the treatment of post-stroke aphasia. Theta burst stimulation (TBS) is a specific pattern of rTMS with shorter stimulation times and better therapeutic effects. The effect of continuous TBS (cTBS) on the cerebellum in patients with aphasia with chronic stroke needs further exploration.

Methods: In this randomized, sham-controlled clinical trial, patients ( = 40) with chronic post-stroke aphasia received 10 sessions of real cTBS ( = 20) or sham cTBS ( = 20) over the right cerebellar Crus I+ a 30-min speech-language therapy. The Western Aphasia Battery (WAB) serves as the primary measure of the treatment outcome. The secondary outcome measures include the Boston Diagnostic Aphasia Examination, Boston Naming Test and speech acoustic parameters. Resting-state fMRI data were also obtained to examine treatment-induced changes in functional connectivity of the cerebro-cerebellar network. These outcome measures are assessed before, immediately after, and 12 weeks after cerebellar cTBS intervention.

Discussion: This protocol holds promise that cerebellar cTBS is a potential strategy to improve language functions in chronic post-stroke aphasia. The resting-state fMRI may explore the neural mechanism underlying the aphasia rehabilitation with cerebellar cTBS.
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http://dx.doi.org/10.3389/fnagi.2022.909733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201405PMC
June 2022

Network Pharmacology-Based Strategy to Investigate the Mechanisms of Lenvatinib in the Treatment of Hepatocellular Carcinoma.

Comput Intell Neurosci 2022 18;2022:7102500. Epub 2022 May 18.

Department of Pharmacy, Minhang Hospital, Fudan University, Shanghai 201199, China.

Hepatocellular carcinoma (HCC) is a complex and refractory malignant tumor, ranking the third cause of cancer-related deaths worldwide. Lenvatinib is currently employed to treat advanced, unresectable HCC as a first-line drug. The purpose of this study was to explore the pharmacological mechanisms of lenvatinib acting on HCC through the analysis of differential expressed genes based on network pharmacology. The target genes of lenvatinib were collected from PubChem, SwissTargetPrediction, PharmMapper, and BATMAN-TCM online public databases. In addition, related gene targets for HCC were obtained using NCBI Gene Expression Omnibus (NCBI-GEO) database. Afterward, the protein-protein interaction (PPI) network was established to visualize and understand the interaction relationships of overlapping gene targets from both lenvatinib and HCC. Furthermore, according to the data obtained, Gene Ontology (GO) analysis indicated that these intersectant genes were mainly enriched in response to xenobiotic stimulus, gland development, ion channel complex, membrane raft, and steroid binding. Besides, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis suggested that the therapeutic effects of lenvatinib on HCC probably involved bile secretion, MAPK signaling pathway, cGMP-PKG signaling pathway, PI3K-Akt signaling pathway, and Ras signaling pathway. Moreover, a total of six key differential genes, namely, ALB, CCND1, ESR1, AR, CCNA2, and AURKA, were identified as most significant targets associated with lenvatinib treating HCC and further verified by molecular docking, which demonstrated that lenvatinib had a strong binding efficiency with these six key gene-encoded proteins. Taken together, this study systematically provided new insights for researchers to determine the intervention mechanisms of lenvatinib in HCC therapy.
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http://dx.doi.org/10.1155/2022/7102500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205703PMC
June 2022

Prognostic Significance and Immunological Role of FBXO5 in Human Cancers: A Systematic Pan-Cancer Analysis.

Front Immunol 2022 3;13:901784. Epub 2022 Jun 3.

Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.

F-box protein 5 (FBXO5), an essential subunit of the ubiquitin protein ligase complex, is increasingly recognized to exhibit important biological effects in regulating tumor occurrence and progression. The present research was intended to systematically investigate the latent roles of FBXO5 in prognosis and immunological function across cancers. Pan-cancer analyses of FBXO5 were performed based upon publicly available online databases, mainly including the Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), UCSC Xena, cBioPortal, and ImmuCellAI, revealing the possible relationships between FBXO5 and prognosis, DNA methylation, tumor microenvironment (TME), infiltration of immune cells, immune-related genes, immune checkpoints, tumor mutation burden (TMB), and microsatellite instability (MSI). The results suggested that FBXO5 was expressed at a high level in numerous tumor cell lines with significant upregulation in most cancers as opposed to normal tissues. Of note, elevated expression of FBXO5 was significantly related to an unfavorable prognosis in many cancer types. Furthermore, DNA methylation and TME were confirmed to display evident correlation with the expression of FBXO5 in several malignancies. Moreover, FBXO5 expression was remarkably positively correlated with the levels of infiltrating Treg cells and Tcm cells in most tumors, but negatively correlated with tumor-infiltrating CD8 T cells, NK/NKT cells, and Th2 cells. Meanwhile, FBXO5 was demonstrated to be co-expressed with the genes encoding immune activating and suppressive factors, chemokines, chemokine receptors, and major histocompatibility complex (MHC). Immune checkpoints, TMB, and MSI were also overtly associated with FBXO5 dysregulation among diverse kinds of cancers. Additionally, the enrichment analyses showed close relationships between FBXO5 expression and the processes related to cell cycle and immune inflammatory response. These findings provided a detailed comprehension of the oncogenic function of FBXO5. Because of its crucial roles in cancer immunity and tumorigenesis, FBXO5 may serve as a novel prognostic indicator and immunotherapeutic target for various malignancies.
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http://dx.doi.org/10.3389/fimmu.2022.901784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203914PMC
June 2022

Optimized Anchor-Modified Peptides Targeting Mutated RAS Are Promising Candidates for Immunotherapy.

Front Immunol 2022 26;13:902709. Epub 2022 May 26.

Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London, United Kingdom.

RAS mutations occur in approximately 20% of all cancers and given their clonality, key role as driver mutation, association with poor prognosis and undruggability, they represent attractive targets for immunotherapy. We have identified immunogenic peptides derived from codon 12 mutant RAS (G12A, G12C, G12D, G12R, G12S and G12V), which bind to HLA-A*02:01 and HLA-A*03:01 and elicit strong peptide-specific CD8+ T cell responses, indicating that there is an effective CD8+ T-cell repertoire against these mutant RAS-derived peptides that can be mobilized. Alterations in anchor residues of these peptides enhanced their binding affinity to HLA-A*02:01 molecules and allowed generation of CD8+ T cells that responded to target cells pulsed with the anchor-modified and also with the original peptide. Cytotoxic T cells generated against these peptides specifically lysed tumor cells expressing mutant RAS. Vaccination of transgenic humanized HLA-A2/DR1 mice with a long peptide encompassing an anchor-modified 9-mer G12V epitope generated CD8+ T cells reactive to the original 9-mer and to a HLA-A*02:01-positive human cancer cell line harboring the G12V mutation. Our data provide strong evidence that mutant RAS can be targeted by immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2022.902709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204602PMC
June 2022

Significance of interstitial fibrosis and p16 in papillary thyroid carcinoma.

Endocr J 2022 Jun 18. Epub 2022 Jun 18.

Medical College of Chinese People's Liberation Army, Beijing 100853, China.

We enrolled 264 patients with papillary thyroid carcinoma (PTC). We performed immunohistochemical detection of p16 and determined the degree of interstitial fibrosis (IF). The expression of p16 was associated with pathological tumor-node-metastasis (pTNM) stage and age (p < 0.05). The overall survival was longer in p16-negative patients (195.73 vs. 181.78 months, p = 0.007). p16 was significantly related to the degree of IF (r = 0.130, p = 0.035). PTC patients with no or mild fibrosis tended to have a larger tumor (p = 0.045). The degree of fibrosis was related to the proportion of papillary structure components (p = 0.025). Univariate and multivariate survival analyses showed that relapse-free survival was longer in patients with moderate/severe IF (p < 0.05). In summary, p16 was correlated with prognosis and IF of PTC. Patients with moderate/severe IF tend to have better prognosis in RFS.
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http://dx.doi.org/10.1507/endocrj.EJ22-0010DOI Listing
June 2022

Critical effect of biodegradation on long-term microplastic weathering in sediment environments: A systematic review.

J Hazard Mater 2022 Jun 3;437:129287. Epub 2022 Jun 3.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.

Microplastic (MP) pollution in global sediment has been intensely studied and recognized as the ultimate sink for residual MPs in terrestrial and aquatic ecosystems. During MP long-term retention in sediments, plastic-degrading bacteria (i.e., Flavobacteriaceae, Bacillus, Rhodobacteraceae, and Desulfobacteraceae) can utilize those MPs as their carbon and energy sources through enzyme (hydrolase and oxidoreductase) reactions, which further alter or transform high molecular weight MP polymers into lower molecular weight biodegradation byproducts (i.e., monomers and oligomers) and release toxic additives. In other words, MPs can act as durable substrates for plastic-degrading bacteria in sediments. However, to date, the biodegradation rates of MPs in sediment environments are still poorly understood due to their limited degradation efficiency. Herein, we review the enzyme-induced biodegradation processes of MPs in sediment environments, which is important for accessing the alteration of MP properties and their potential ecological risks after undergoing long-term weathering processes. In addition, the factors associated with the MP properties (polymer type, molecular weight, crystallinity, and hydrophobicity) and sediment conditions (sediment type, temperature, pH, salinity, and oxygen content) that influence plastic degradation processes are also reviewed. The mechanisms may relate to the MP properties and sediment conditions that can influence microbial abundance, enzyme concentrations, and enzyme activities, thus altering MP biodegradation ratios. We anticipate that the observations reviewed in this study will pose a new issue to better understand the formation process, fate, and potential ecological risks associated with aged MPs in sediment environments.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129287DOI Listing
June 2022

Identification of Prognosis-Related Molecular Subgroups and Construction of a Prognostic Prediction Model Using Immune-Related Genes in Pancreatic Cancer.

J Oncol 2022 7;2022:7117014. Epub 2022 Jun 7.

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, China.

Background: Pancreatic cancer patients with similar clinicopathological status exhibit substantially different therapeutic responses, which might be caused by the vast molecular heterogeneity of tumors. In this study, we attempted to identify specific molecular subgroups and construct a prognostic prediction model based on the expression level of immune-related genes in pancreatic cancer. The transcriptome profiling, single nucleotide variation, copy number variation, clinicopathological information, and follow-up data of pancreatic cancer patients were obtained from The Cancer Genome Atlas database. Thereafter, the immune-related genes with prognostic significance were identified for further consensus cluster analysis. The molecular characteristics and clinicopathological information were compared between the identified subgroups, and a weighted correlation network analysis was performed to identify the hub genes associated with the subgroups. Finally, the prognostic prediction model based on immune-related genes was established using the least absolute shrinkage and selection operator (LASSO) analysis.

Results: A total of 67 immune-relevant genes with prognostic significance were selected and used for the consensus cluster analysis. The total samples were divided into two groups, C1 and C2. The subgroup C1 had a significantly worse prognosis than C2, as well as lower levels of immune cell infiltration, which indicate an immunosuppressed state. The mutational rate of the cancer-related genes including , 53, and 43 was higher in the C1 subgroup. The C1 subgroup was associated with more advanced tumor grade and T stage and with higher mortality. Using LASSO regression, we developed a prognostic prediction model based on the expression levels of 19 immune-related genes, which we validated in three external data sets. In addition, we identified four potential therapeutic and prognostic biomarkers (, , , and ).

Conclusion: We identified two novel molecular subgroups of pancreatic cancer and developed a prognostic prediction model based on the expression levels of immune-related genes, which could be used in a clinical setting and could aid in unraveling the molecular processes leading to the development of pancreatic cancer.
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http://dx.doi.org/10.1155/2022/7117014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197625PMC
June 2022

Autofluorescence detection and co-axial projection for intraoperative localization of parathyroid gland.

Biomed Eng Online 2022 Jun 16;21(1):37. Epub 2022 Jun 16.

Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, 230026, China.

Background: Near-infrared (NIR) autofluorescence detection is an effective method for identifying parathyroid glands (PGs) in thyroidectomy or parathyroidectomy. Fiber optical probes provide quantitative autofluorescence measurements for PG detection owing to its high sensitivity and high excitation light cut-off efficiency at a fixed detection distance. However, an optical fiber probe lacks the imaging capability and cannot map the autofluorescence distribution on top of normal tissue background. Therefore, there is a need for intraoperative mapping of PGs with high sensitivity and imaging resolution.

Methods: We have developed a fluorescence scanning and projection (FSP) system that combines a scanning probe and a co-axial projector for intraoperative localization and in situ display of PGs. Some of the key performance characteristics, including spatial resolution and sensitivity for detection, spatial resolution for imaging, dynamic time latency, and PG localization capability, are characterized and verified by benchtop experiments. Clinical utility of the system is simulated by a fluorescence-guided PG localization surgery on a tissue-simulating phantom and validated in an ex vivo experiment.

Results: The system is able to detect indocyanine green (ICG) solution of 5 pM at a high signal-to-noise ratio (SNR). Additionally, it has a maximal projection error of 0.92 mm, an averaged projection error of 0.5 ± 0.23 mm, and an imaging resolution of 748 μm at a working distance ranging from 35 to 55 cm. The dynamic testing yields a short latency of 153 ± 54 ms, allowing for intraoperative scanning on target tissue during a surgical intervention. The simulated fluorescence-guided PG localization surgery has validated the system's capability to locate PG phantom with operating room ambient light interference. The simulation experiment on the PG phantom yields a position detection bias of 0.36 ± 0.17 mm, and an area intersection over unit (IoU) of 76.6% ± 6.4%. Fluorescence intensity attenuates exponentially with the thickness of covered tissue over the PG phantom, indicating the need to remove surrounding tissue in order to reveal the weak autofluorescence signal from PGs. The ex vivo experiment demonstrates the technical feasibility of the FSP system for intraoperative PG localization with accuracy.

Conclusion: We have developed a novel probe-based imaging and navigation system with high sensitivity for fluorescence detection, capability for fluorescence image reconstruction, multimodal image fusion and in situ PG display function. Our studies have demonstrated its clinical potential for intraoperative localization and in situ display of PGs in thyroidectomy or parathyroidectomy.
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http://dx.doi.org/10.1186/s12938-022-01004-8DOI Listing
June 2022

Effect of Nrf2 on Phenotype Changes of Macrophages in the Anterior Vaginal Wall of Women With Pelvic Organ Prolapse.

Female Pelvic Med Reconstr Surg 2022 May 31. Epub 2022 May 31.

From the Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Zhengzhou University.

Objective: The aim of this study was to observe the effect of nuclear factor-erythroid 2-related factor 2 (Nrf2) on the phenotype changes of macrophages in the anterior vaginal wall of patients with pelvic organ prolapse (POP).

Methods: The tissues of the anterior vaginal wall of the control group (n = 30) and POP groups (n = 60) were collected during operation. The expressions of Nrf2, iNOS (representative factor of M1 macrophages), and CD206 (representative factor of M2 macrophages) were determined by immunohistochemical staining and Western blot. Morphological changes and collagen distribution of the anterior vaginal wall were observed by hematoxylin-eosin staining and Masson trichrome staining.

Results: Compared with the control group, the expression levels of Nrf2 and CD206 protein in the anterior vaginal wall tissues of the POP groups were significantly decreased (P < 0.05), and were negatively proportional to the degree of prolapse (P < 0.05). The expression of iNOS was significantly increased and was directly proportional to the degree of prolapse (P < 0.05). Hematoxylin-eosin staining and Masson trichrome staining showed that the collagen fibers are more sparsely arranged and disordered in the POP group than the control.

Conclusions: In patients with POP, the expression of antioxidant factor Nrf2 is reduced in the vaginal anterior wall tissues and the antioxidant capacity is weakened, leading to the blocked polarization of macrophages and the accumulation of a large number of M1 macrophages in the tissue, affecting the occurrence and development of POP.
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http://dx.doi.org/10.1097/SPV.0000000000001212DOI Listing
May 2022

Design, Synthesis and Anti-Tumor Activity Evaluation of Novel 3,4-(Methylenedioxy)cinnamic Acid Amide-Dithiocarbamate Derivatives.

Chem Biodivers 2022 Jun 15:e202200439. Epub 2022 Jun 15.

School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, 330006, P. R. China.

The fragments, 3,4-(methylenedioxy)cinnamic acid amide and dithiocarbamates, have received increasing attention because of their multiple pharmacological activities in recent years, especially in anti-tumor. We synthesized 17 novel 3,4-(methylenedioxy)cinnamic acid amide-dithiocarbamate derivatives based on the principle of pharmacophore assembly and discovered that compound 4a displayed the most potent antiproliferative activity against HeLa cells with IC value of 1.01 μM. Further mechanistic studies revealed that 4a triggered apoptosis in HeLa cells via activating mitochondria-mediated intrinsic pathways and effectively inhibited colony formation. Also, 4a had the ability to arrest cell cycle in the G2/M phase as well as to inhibit the migration in HeLa cells. More importantly, acute toxicity experiments showed that 4a had good safety in vivo. All the results suggested that compound 4a might serve as a promising lead compound that merited further attention in future anti-tumor drug discovery.
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http://dx.doi.org/10.1002/cbdv.202200439DOI Listing
June 2022

Predicting Survival for Hepatic Arterial Infusion Chemotherapy of Unresectable Colorectal Liver Metastases: Radiomics Analysis of Pretreatment Computed Tomography.

J Transl Int Med 2022 Mar 2;10(1):56-64. Epub 2022 Apr 2.

Department of Radiology, Peking University Cancer Hospital and Institute, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.

Objective: Hepatic arterial infusion chemotherapy (HAIC) is an effective treatment for advanced unresectable colorectal cancer liver metastases (CRLM). This study was conducted to predict the efficacy of HAIC in patients with unresectable CRLM by radiomics methods based on pretreatment computed tomography (CT) examinations and clinical data.

Materials And Methods: A total of 63 patients were included in this study (41 in the training group and 22 in the validation group). All these patients underwent CT examination before HAIC. During the follow-up period, CT scans and laboratory examinations were performed regularly. Eighty-five radiological features were extracted from the regions of interest (ROIs) of CT images using the PyRadiomics program. The -test and correlation were applied to select features. These features were analyzed using LASSO-Cox regression, and a linear model was developed to predict overall survival (OS).

Results: After reducing features by -test and correlation test, seven features remained. After LASSO-Cox cross-validation, four features remained at λ = 0.232. They were gray level co-occurrence matrix (GLCM), gray level run length matrix (GLRLM), neighborhood gray tone difference matrix (NGTDM), and the location of the primary tumor. The C-index was 0.758 in the training group and 0.743 in the test group. Nomograms predicting 1-, 2-, and 3-year survival were established.

Conclusion: Our study demonstrates that a radiomics approach based on pretreatment CT texture analysis has the ability to predict early the outcome of HAIC in patients with advanced unresectable colorectal cancer with a high degree of accuracy and feasibility.
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http://dx.doi.org/10.2478/jtim-2022-0004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997799PMC
March 2022
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