Publications by authors named "Peng Li"

4,286 Publications

  • Page 1 of 1

Insulin-induced conformational changes in the full-length insulin receptor: structural insights gained from molecular modeling analyses.

Acta Biochim Biophys Sin (Shanghai) 2021 May 14. Epub 2021 May 14.

Shenzhen Key Laboratory of Steroid Drug Discovery and Development, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen 518172, China.

Insulin receptor plays an important role in the regulation of energy metabolism. Dysfunction of insulin receptor (IR) can lead to many disease states, such as diabetes mellitus. Deciphering the complex dynamic structures of human IR and its mechanism of activation would greatly aid in understanding IR-mediated signaling pathways and also in designing new drugs (including nonpeptidal insulin analogs) to treat diabetes mellitus. Experimental evidence about IR structures has been gradually obtained by biologists over the past three decades. Based on available experimental structures of IR in different states, here we employ molecular modeling approach to construct the full-length IR structures in different states and model its structural and conformational changes during insulin-induced IR activation. Several key possible intermediate states are constructed based on structural alignment, rotation, and computational modeling. Based on the structures of the full-length IR in different states, it appears that there are two possible conformational transition pathways: one is symmetric and the other one is asymmetric. Structural changes and motions of different domains of the full-length IR along the pathways are analyzed. The role of insulin binding to IR in facilitating the conformational transition of the receptor is analyzed. Information and insights derived from our present structural modeling analyses may aid in understanding the complex dynamic, structural, and conformational changes during the process of IR activation.
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http://dx.doi.org/10.1093/abbs/gmab053DOI Listing
May 2021

miR-9-5p promotes wear-particle-induced osteoclastogenesis through activation of the SIRT1/NF-κB pathway.

3 Biotech 2021 Jun 9;11(6):258. Epub 2021 May 9.

Department of Orthoped, General Hospital of Ningxia Medical University, No. 804 Shengli South Street, Xingqing District, Yinchuan, 750004 Ningxia Province China.

To explore the potential function of miR-9-5p in wear-particle-induced osteoclastogenesis, we examined the expression of SIRT1 and miR-9-5p in particle-induced osteolysis (PIO) mice calvariae and polyethylene (PE)-induced RAW 264.7 cells and found that SIRT1 expression was downregulated while miR-9-5p expression was upregulated in both models. We then verified that miR-9-5p targets SIRT1. miR-9-5p was found to promote PE-induced osteoclast formation from RAW 264.7 cells by staining and detection of osteoclast markers, and miR-9-5p activation of the SIRT1/NF-kB signaling pathway was found in cells by detecting the expression of SIRT1/NF-kB pathway-related proteins and rescue assays. In conclusion, we found that miR-9-5p activated the SIRT1/NF-κB pathway to promote wear-particle-induced osteoclastogenesis. miR-9-5p may be a useful therapeutic target for PIO remission and treatment.
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http://dx.doi.org/10.1007/s13205-021-02814-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107064PMC
June 2021

aFGF Targeted Mediated by Novel Nanoparticles-Microbubble Complex Combined With Ultrasound-Targeted Microbubble Destruction attenuates Doxorubicin-Induced Heart Failure via Anti-Apoptosis and Promoting Cardiac Angiogenesis.

Front Pharmacol 2021 27;12:607785. Epub 2021 Apr 27.

Henan International Joint Laboratory of Cardiovascular Remodeling and Drug Intervention, Xinxiang, China.

The purpose of this study was to evaluate the protective effect of acidic fibroblast growth factor targeted mediated by novel nanoparticles-cationic lipid microbubbles complex (aFGF-NP + CPMBs) combined with ultrasound targeted microbubble destruction (UTMD)on doxorubicin-induced heart failure (HF)and its mechanism. Heart failure rats induced by intraperitoneal injection with doxorubicin (DOX) to achieve cummulative dose of 15mg/kg for continuous 6 weeks showed left ventricular dysfunction, seriously oxidative stress, cardiomyocyte apoptosis, and decrease of myocardial vascular density. In contrast, aFGF-NP + CPMBs combined with UTMD therapy (3ug/kg, caudal vein injection, twice a week, 6weeks)prominently ameliorated left ventricular dysfunction by increased ejection fraction (EF) and fractional shortening (FS), decreased brain natriuretic peptide (BNP); strengthened the ability of antioxidant stress confirmed by increasing the activity of SOD and reducing the production of MDA; exerted the effect of anti-cardiomyocyte apoptosis and promotion angiogenesis by inhibited Bax expression and increased Bcl-2 expression and platelet endothelial cell adhesion molecule (CD31) expression. Taken together, the research suggested that aFGF targeted mediated by novel nanoparticles-cationic lipid microbubbles complex combined with UTMD should be a promising targeted treatment for heart failure.
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http://dx.doi.org/10.3389/fphar.2021.607785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111001PMC
April 2021

Glycolytic ATP fuels phosphoinositide 3-kinase signaling to support effector T helper 17 cell responses.

Immunity 2021 May;54(5):976-987.e7

Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Immunology and Microbial Pathogenesis Graduate Program, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, NY 10065, USA; Louis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Aerobic glycolysis-the Warburg effect-converts glucose to lactate via the enzyme lactate dehydrogenase A (LDHA) and is a metabolic feature of effector T cells. Cells generate ATP through various mechanisms and Warburg metabolism is comparatively an energy-inefficient glucose catabolism pathway. Here, we examined the effect of ATP generated via aerobic glycolysis in antigen-driven T cell responses. Cd4Ldha mice were resistant to Th17-cell-mediated experimental autoimmune encephalomyelitis and exhibited defective T cell activation, migration, proliferation, and differentiation. LDHA deficiency crippled cellular redox balance and inhibited ATP production, diminishing PI3K-dependent activation of Akt kinase and thereby phosphorylation-mediated inhibition of Foxo1, a transcriptional repressor of T cell activation programs. Th17-cell-specific expression of an Akt-insensitive Foxo1 recapitulated the defects seen in Cd4Ldha mice. Induction of LDHA required PI3K signaling and LDHA deficiency impaired PI3K-catalyzed PIP3 generation. Thus, Warburg metabolism augments glycolytic ATP production, fueling a PI3K-centered positive feedback regulatory circuit that drives effector T cell responses.
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http://dx.doi.org/10.1016/j.immuni.2021.04.008DOI Listing
May 2021

Hierarchical TiCT MXene/Carbon Nanotubes for Low Overpotential and Long-Life Li-CO Batteries.

ACS Nano 2021 May 12. Epub 2021 May 12.

Jiangsu Key Laboratory of Electrochemical Energy Storage Technologies, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.

Electrochemical carbon dioxide conversion at ambient temperature is an efficient route to synchronously provide a continuous power supply and produce useful chemicals such as carbonates. Rigid catalysts with rational morphological and structural design are used to overcome the sluggish reaction kinetics and contribute to a better cycle life in Li-CO batteries. In this report, a two-dimensional TiCT MXene/carbon heterostructure assembled parallel-aligned tubular architecture was delicately synthesized through a self-sacrificial templating method and delivered an ultralow overpotential of 1.38 V at 0.2 A·g. The heterostructure that inherited the high catalytic performance of TiCT MXene and the outstanding stability of carbon material promoted the adsorption of CO and accelerated the decomposition of lithium carbonate, which was proved by and characterizations and density functional theory calculations. The tubular architecture with large surface area was demonstrated to provide a high durability for long cycle life and ensure good contacts among gas, electrolyte, and electrode.
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http://dx.doi.org/10.1021/acsnano.0c10558DOI Listing
May 2021

Surface engineering of linearly fused Au units using diphosphine and Cd doping.

Chem Commun (Camb) 2021 May;57(38):4682-4685

Department of Chemistry and Center for Atomic Engineering of Advanced Materials, Institutes of Physical Science and Information Technology, Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University), Ministry of Education, Hefei, Anhui 230601, P. R. China.

Herein, surface engineering was delicately performed to assemble two new Au-Cd alloy nanoclusters, including [Cd2Au17(S-c-C6H11)12(DPPP)2](BPh4) and Cd2Au29(TBBT)17(DPPF)2. Both the Au13 (in Cd2Au17) and Au25 (in Cd2Au29) cores were covered by two identical Au2Cd(SR)6 motifs and two diphosphine ligands. In addition, their optical properties were explored to give clues on the kernel- and surface-dependent electronic structures.
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http://dx.doi.org/10.1039/d1cc00577dDOI Listing
May 2021

Ultra-Conformable Ionic Skin with Multi-Modal Sensing, Broad-Spectrum Antimicrobial and Regenerative Capabilities for Smart and Expedited Wound Care.

Adv Sci (Weinh) 2021 05 15;8(9):2004627. Epub 2021 Feb 15.

Orthopedic Institute and Department of Orthopedics The First Affiliated Hospital, Soochow University Suzhou Jiangsu 215006 P. R. China.

While rapid wound healing is essential yet challenging, there is also an unmet need for functional restoration of sensation. Inspired by natural skin, an ultra-conformable, adhesive multi-functional ionic skin (MiS) with multi-modal sensing capability is devised for smart and expedited wound care. The base of MiS is a unique skin-like, conductive and self-adaptive adhesive polyacrylamide/starch double-network hydrogel (PSH) and self-powered, flexible, triboelectric sensor(s) is integrated on top of PSH for multi-tactile sensing. MiS could enhance wound contraction, collagen deposition, angiogenesis, and epidermis formation in a full-thickness skin defect wound model , while significantly inhibiting the biofilm formation of a wide range of microorganisms. MiS also exhibits multi-modal sensing capability for smart and instant therapeutics and diagnostics, including skin displacement or joint motion, temperature, pressure and tissue exudate changes of wound bed, and locally releasing drugs in a pH-responsive manner. More importantly, MiS could restore the skin-mimicking tactile sensing function of both touch location and intensity, and thus could be used as a human-machine interface for accurate external robotic control. MiS demonstrates a new comprehensive paradigm of combining wound diagnosis and healing, broad-spectrum anti-microbial capability and restoration of multi-tactile sensing for the reparation of severe wound.
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http://dx.doi.org/10.1002/advs.202004627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097371PMC
May 2021

Characteristics of Metabolites by Seed-Specific Inhibition of in L.

J Agric Food Chem 2021 May 9. Epub 2021 May 9.

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.

Fatty acid desaturase-2 (FAD2) is a key enzyme in the production of polyunsaturated fatty acids in plants. RNAi technology can reduce the expression of genes in seeds and acquire transgenic plants with a high oleic acid content, but the effect of seed-specific inhibition of expression on seed metabolites is not clear. Here we use widely targeted metabolomics to investigate the metabolites of normal-oleic-acid rapeseed (OA) and high-oleic-acid rapeseed (HOA) seeds, resulting in a total of 726 metabolites being detected. Among them, 24 differential metabolites were significantly downregulated and 88 differential metabolites were significantly upregulated in HOA rapeseed. In further lipid profile experiments, more lipids in seeds were accurately quantified. The contents of glycolipids and phospholipids that contain C18:1 increased significantly and C18:2 decreased because expression was inhibited. The changes in the expression of key genes in related pathways were also consistent with the changes in metabolites. The insertion site of the ihpRNA plant expression vector was reconfirmed through genomewide resequencing, and the transgenic event did not change the sequence of genes. There was no significant difference in the germination rate and germination potential between OA and HOA rapeseed seeds because the seed-specific ihpRNA plant expression vector did not affect other stages of plant growth. This work provides a theoretical and practical guidance for subsequent molecular breeding of high OA .
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http://dx.doi.org/10.1021/acs.jafc.0c06867DOI Listing
May 2021

Pulmonary arterial hypertension in a patient with hereditary hemorrhagic telangiectasia and family gene analysis: A case report.

World J Clin Cases 2021 May;9(13):3079-3089

Department of Pulmonary and Critical Care Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.

Background: Hereditary hemorrhagic telangiectasia (HHT) is a rare autosomal dominant genetic disease. Very few patients suffering from HHT present with associated pulmonary arterial hypertension (PAH), which may result in a poor prognosis. Here, we report a case of HHT with PAH. The patient's clinical manifestations and treatment as well as genetic analysis of family members are reviewed, in order to raise awareness of this multimorbidity.

Case Summary: A 45-year-old Chinese woman was admitted to the hospital to address a complaint of intermittent shortness of breath, which had lasted over the past 2 years. She also had a 30-year history of recurrent epistaxis and 5-year history of anemia. She reported that the shortness of breath had aggravated gradually over the 2 years. Physical examination discovered anemia and detected gallop rhythm in the precordium. Chest computerized tomography and cardiac ultrasound demonstrated PAH and hepatic arteriovenous malformation. The formal clinical diagnosis was HHT combined with PAH. The patient was treated with ambrisentan and her condition improved for a time. She died half a year after the diagnosis. Genetic testing revealed the patient and some family members to carry an activin A receptor-like type 1 mutation (c. 1232G>A, p. Arg411Gln); the family was thus identified as an HHT family.

Conclusion: We report a novel gene mutation (c. 1232G>A, p. Arg411Gln) in a Chinese HHT patient with PAH.
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http://dx.doi.org/10.12998/wjcc.v9.i13.3079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080754PMC
May 2021

Endoscopic Transcanal Push-Trough Myringoplasty for Different Types of Tympanic Membrane Perforations.

Otol Neurotol 2021 Jun;42(5):726-732

Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou.

Objective: This study aims to explore the feasibility of endoscopic transcanal push-trough myringoplasty (ETPM) for all types of tympanic membrane perforations (TMPs), and compare the outcomes with those of endoscopic type 1 tympanoplasty (ETT) with meatal flap elevation.

Study Design: A prospective, controlled study.

Methods: In the present study, inpatients with TMPs were divided into two groups according to the manner of tympanic membrane repair: one group received ETPM without raising the meatal flap, and the other received ETT. The operation duration, postoperative healing rate, visual analogue scale (VAS) of pain, and complication rates were compared between these two groups.

Results: Regardless of the size and location of the perforation, and its relationship with the malleus manubrium, myringoplasty can be completed using the ETPM method. The operation duration for different types of TMPs was shorter in the ETPM group than in the ETT group, and the difference was statistically significant (p<0.05). However, there was no statistical difference in the short-term healing rate (p>0.05) and pain VAS score (p>0.05) between these two groups. Furthermore, no intraoperative or postoperative complications occurred in both groups.

Conclusion: ETPM without raising the meatal flap can be applied for all types of TMPs, regardless of how large the perforation is, or where it is located. This can shorten the operation duration, and has a high healing rate comparable to ETT and mild postoperative pain. Mastering some essential surgical skills under the endoscope would be helpful to ensure the success of the surgery.
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http://dx.doi.org/10.1097/MAO.0000000000003029DOI Listing
June 2021

ROC1 promotes the malignant progression of bladder cancer by regulating p-IκBα/NF-κB signaling.

J Exp Clin Cancer Res 2021 May 7;40(1):158. Epub 2021 May 7.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No.100 Haining Road, Hongkou District, Shanghai, 200080, China.

Background: Regulator of cullins 1 (ROC1) is an important catalytic subunit of cullin-RING E3 ligase. Nuclear factor-kappa B (NF-κB) signaling is closely related to tumor invasion and metastasis. Earlier, we reported that ROC1 was associated with a poor prognosis in patients with bladder cancer (BCa). However, it is unclear whether ROC1 is involved in the NF-κB signaling associated with malignant BCa progression.

Methods: The expression of ROC1 and p65 in bladder cancer and paracancerous tissues were detected by immunohistochemistry (IHC). Pearson correlation was used to assess correlation between ROC1 and p65 protein expressions. The wound-healing and transwell assays were used to monitor cell invasion and migration. The effect of ROC1 on the expression of key proteins in the NF-κB signaling was determined by immunofluorescence and western blot (WB). Cycloheximide (CHX), MG132 and immunoprecipitation assays were used to evaluate the effect of ROC1 on the ubiquitination of phosphorylated inhibitor of kappa B alpha (p-IκBα). A lung metastasis mouse model was generated to detect the role of ROC1 in tumor metastasis.

Results: We found that ROC1 was up-regulated in BCa tissues and cell lines, and high ROC1 levels were positively correlated with higher tumour grade, lymph node metastasis, distant metastasis and poor prognosis. Linear-regression analysis showed significant a Pearson correlation between ROC1 and nuclear p65 expression in BCa tissue microarray (TMA) samples. Functional studies demonstrated that ROC1 promoted BCa cell invasion and migration. In vitro and in vivo experiments showed that ROC1 activated NF-κB signaling by enhancing the ubiquitination of p-IκBα, which caused p65 nuclear translocation and promoted the transcription of some metastasis-related target genes, such as urokinase-type plasminogen activator receptor (uPAR), intracellular adhesion molecule 1 (ICAM1), vascular cell adhesion molecule 1 (VCAM1), and matrix metalloproteinase 9 (MMP9), resulting in promoting BCa metastasis.

Conclusion: ROC1 plays an important role in the progression of BCa and serves as a potential diagnostic and therapeutic target for patients with BCa.
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http://dx.doi.org/10.1186/s13046-021-01935-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106150PMC
May 2021

Characterization of the gut DNA and RNA Viromes in a Cohort of Chinese Residents and Visiting Pakistanis.

Virus Evol 2021 Jan 24;7(1):veab022. Epub 2021 Mar 24.

School of Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Road, Qixia District, Nanjing 210029, China.

Trillions of viruses inhabit the gastrointestinal tract. Some of them have been well-studied on their roles in infection and human health, but the majority remains unsurveyed. It has been established that the composition of the gut virome is highly variable based on the changes of diet, physical state, and environmental factors. However, the effect of host genetic factors, for example ethnic origin, on the gut virome is rarely investigated. Here, we characterized and compared the gut virome in a cohort of local Chinese residents and visiting Pakistani individuals, each group containing twenty-four healthy adults and six children. Using metagenomic shotgun sequencing and assembly of fecal samples, a huge number of viral operational taxonomic units (vOTUs) were identified for profiling the DNA and RNA viromes. National background contributed a primary variation to individuals' gut virome. Compared with the Chinese adults, the Pakistan adults showed higher macrodiversity and different compositional and functional structures in their DNA virome and lower diversity and altered composition in their RNA virome. The virome variations of Pakistan children were not only inherited from that of the adults but also tended to share similar characteristics with the Chinese cohort. We also analyzed and compared the bacterial microbiome between two cohorts and further revealed numerous connections between viruses and bacterial host. Statistically, the gut DNA and RNA viromes were covariant to some extent (<0.001), and they both correlated the holistic bacterial composition and vice versa. This study provides an overview of the gut viral community in Chinese and visiting Pakistanis and proposes a considerable role of ethnic origin in shaping the virome.
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http://dx.doi.org/10.1093/ve/veab022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087960PMC
January 2021

Chronobiology of Natriuretic Peptides and Blood Pressure in Lean and Obese Individuals.

J Am Coll Cardiol 2021 May;77(18):2291-2303

Division of Cardiovascular Disease, University of Alabama at Birmingham, Birmingham, Alabama, USA; Section of Cardiology, Birmingham Veterans Affairs Medical Center, Birmingham, Alabama, USA. Electronic address:

Background: Diurnal variation of natriuretic peptide (NP) levels and its relationship with 24-h blood pressure (BP) rhythm has not been established. Obese individuals have a relative NP deficiency and disturbed BP rhythmicity.

Objectives: This clinical trial evaluated the diurnal rhythmicity of NPs (B-type natriuretic peptide [BNP], mid-regional pro-atrial natriuretic peptide [MR-proANP], N-terminal pro-B-type natriuretic peptide [NT-proBNP]) and the relationship of NP rhythm with 24-h BP rhythm in healthy lean and obese individuals.

Methods: On the background of a standardized diet, healthy, normotensive, lean (body mass index 18.5 to 25 kg/m) and obese (body mass index 30 to 45 kg/m) individuals, age 18 to 40 years, underwent 24-h inpatient protocol involving ambulatory BP monitoring starting 24 h prior to the visit, controlled light intensity, and repeated blood draws for assessment of analytes. Cosinor analysis of normalized NP levels (normalized to 24-h mean value) was conducted to assess the diurnal NP rhythm and its relationship with systolic BP.

Results: Among 52 participants screened, 40 participants (18 lean, 22 obese; 50% women; 65% Black) completed the study. The median range spread (percentage difference between the minimum and maximum values) over 24 h for MR-proANP, BNP, and NT-proBNP levels was 72.0% (interquartile range [IQR]: 50.9% to 119.6%), 75.5% (IQR: 50.7% to 106.8%), and 135.0% (IQR: 66.3% to 270.4%), respectively. A cosine wave-shaped 24-h oscillation of normalized NP levels (BNP, MR-proANP, and NT-proBNP) was noted both in lean and obese individuals (p <0.05 for all). A larger phase difference between MR-proANP BP rhythm (-4.9 h vs. -0.7 h) and BNP BP rhythm (-3.3 h vs. -0.9 h) was seen in obese compared with lean individuals.

Conclusions: This human physiological trial elucidates evidence of diurnal NP rhythmicity and the presence of an NP-BP rhythm axis. There exists a misalignment of the NP-BP diurnal rhythm in the obese, which may contribute to the disturbed diurnal BP pattern observed among obese individuals. (The Diurnal Rhythm in Natriuretic Peptide Levels; NCT03834168).
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http://dx.doi.org/10.1016/j.jacc.2021.03.291DOI Listing
May 2021

miR-378c suppresses Wilms tumor development via negatively regulating CAMKK2.

Acta Biochim Biophys Sin (Shanghai) 2021 May 6. Epub 2021 May 6.

Department of Paediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

Wilms tumor is a rare kidney malignancy primarily developed in children. Treatment for Wilms tumor includes surgery, radiotherapy, and chemotherapy. Recent studies have demonstrated that microRNAs (miRNAs) play important roles in regulating Wilms tumor development. In this study, we aimed to elucidate the expression and function of miR-378c in Wilms tumor. Quantitative real-time PCR (qRT-PCR) results showed that miR-378c was downregulated in Wilms tumor tissues and cell lines. Functionally, further CCK-8, would healing, and transwell assays revealed that overexpression of miR-378c impaired Wilms tumor cell growth and metastasis in vitro. In addition, xenograft assay showed that miR-378c overexpression inhibited Wilms tumor development in vivo. Mechanistically, luciferase reporter assay confirmed that miR-378c directly targets CAMKK2 in Wilms tumor. qRT-PCR and western blot assays demonstrated that CAMKK2 was highly expressed in Wilms tumor tissues and cell lines. Rescue experiments were performed to further evaluate the functional relationship between miR-378c and CAMKK2. Overexpression of miR-378c suppressed Wilms tumor cell metastasis via negatively regulating CAMKK2 expression. Consistently, inhibition of miR-378c enhanced Wilms tumor cell malignancy behavior via augmenting CAMKK2 expression, which could be abrogated by CAMKK2 knockdown. In summary, our findings suggest that miR-378c inhibits the development and metastasis of Wilms tumor via negatively regulating CAMKK2 expression, which could be utilized to develop new therapy strategy.
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http://dx.doi.org/10.1093/abbs/gmab047DOI Listing
May 2021

Weighted-Attribute Triplet Hashing for Large-Scale Similar Judicial Case Matching.

Comput Intell Neurosci 2021 16;2021:6650962. Epub 2021 Apr 16.

School of Software, Shandong University, Jinan, China.

Similar judicial case matching aims to enable an accurate selection of a judicial document that is most similar to the target document from multiple candidates. The core of similar judicial case matching is to calculate the similarity between two fact case documents. Owing to similar judicial case matching techniques, legal professionals can promptly find and judge similar cases in a candidate set. These techniques can also benefit the development of judicial systems. However, the document of judicial cases not only is long in length but also has a certain degree of structural complexity. Meanwhile, a variety of judicial cases are also increasing rapidly; thus, it is difficult to find the document most similar to the target document in a large corpus. In this study, we present a novel similar judicial case matching model, which obtains the weight of judicial feature attributes based on hash learning and realizes fast similar matching by using a binary code. The proposed model extracts the judicial feature attributes vector using the bidirectional encoder representations from transformers (BERT) model and subsequently obtains the weighted judicial feature attributes through learning the hash function. We further impose triplet constraints to ensure that the similarity of judicial case data is well preserved when projected into the Hamming space. Comprehensive experimental results on public datasets show that the proposed method is superior in the task of similar judicial case matching and is suitable for large-scale similar judicial case matching.
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http://dx.doi.org/10.1155/2021/6650962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064799PMC
April 2021

Genomic elucidation of a COVID-19 resurgence and local transmission of SARS-CoV-2 in Guangzhou, China.

J Clin Microbiol 2021 May 5. Epub 2021 May 5.

Beijing Institute of Radiation Medicine, Beijing, China

While China experienced a peak and decline in coronavirus disease 2019 (COVID-19) cases at the start of 2020, in subsequent months, regional outbreaks have continuously emerged. Resurgences of COVID-19 have also been observed in many other countries. In Guangzhou, China, a small outbreak, involving less than 100 residents, emerged in March and April 2020, and comprehensive and near-real-time genomic surveillance of SARS-CoV-2 was conducted. When the numbers of confirmed cases among overseas travelers increased, public health measures were enhanced by shifting from self-quarantine to central quarantine and SARS-CoV-2 testing for all overseas travelers. In an analysis of 109 imported cases, we found diverse viral variants distributed in the global viral phylogeny, which were frequently shared within households but not among passengers on the same flight. In contrast to the viral diversity of imported cases, local transmission was predominately attributed to two specific variants imported from Africa, including local cases that reported no direct or indirect contact with imported cases. The introduction events of the virus were identified or deduced before the enhanced measures were taken. These results show that the interventions were effective in containing the spread of SARS-CoV-2, and they ruled out the possibility of cryptic transmission of viral variants from the first wave in January and February 2020. Our study provides evidence and emphasizes the importance of controls for overseas travelers in the context of the pandemic and exemplifies how viral genomic data can facilitate COVID-19 surveillance and inform public health mitigation strategies.
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http://dx.doi.org/10.1128/JCM.00079-21DOI Listing
May 2021

Forensic nanopore sequencing of STRs and SNPs using Verogen's ForenSeq DNA Signature Prep Kit and MinION.

Int J Legal Med 2021 May 5. Epub 2021 May 5.

School of Forensic Medicine, Shanxi Medical University, Taiyuan, 030001, People's Republic of China.

The MinION nanopore sequencing device (Oxford Nanopore Technologies, Oxford, UK) is the smallest commercially available sequencer and can be used outside of conventional laboratories. The use of the MinION for forensic applications, however, is hindered by the high error rate of nanopore sequencing. One approach to solving this problem is to identify forensic genetic markers that can consistently be typed correctly based on nanopore sequencing. In this pilot study, we explored the use of nanopore sequencing for single nucleotide polymorphism (SNP) and short tandem repeat (STR) profiling using Verogen's (San Diego, CA, USA) ForenSeq DNA Signature Prep Kit. Thirty single-contributor samples and DNA standard material 2800 M were genotyped using the Illumina (San Diego, CA, USA) MiSeq FGx and MinION (with R9.4.1 flow cells) devices. With an optimized cutoff for allelic imbalance, all 94 identity-informative SNP loci could be genotyped reliably using the MinION device, with an overall accuracy of 99.958% (1 error among 2926 genotypes). STR typing was notably error prone, and its accuracy was locus dependent. We developed a custom-made bioinformatics workflow, and finally selected 13 autosomal STRs, 14 Y-STRs, and 4 X-STRs showing high consistency between nanopore and Illumina sequencing among the tested samples. These SNP and STR loci could be candidates for panel design for forensic analysis based on nanopore sequencing.
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http://dx.doi.org/10.1007/s00414-021-02604-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098014PMC
May 2021

Studies on chemoselective synthesis of 1,4- and 1,2-dihydropyridine derivatives by a Hantzsch-like reaction: a combined experimental and DFT study.

Org Biomol Chem 2021 May;19(17):3882-3892

Beijing Key Laboratory of Environmental and Viral Oncology, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, P. R. China.

In the experimental process of preparing diethyl 3,5-dicarboxylate-1,4-dihydropyridine (1,4-DHP) by a Hantzsch-like reaction, it was found that a by-product named diethyl 3,5-dicarboxylate-1,2-dihydropyridine (1,2-DHP) was produced in the reaction. To discuss this phenomenon, the effects of the reaction conditions on the yield ratio of 1,4-DHP and 1,2-DHP were studied by using aromatic amines, aromatic aldehydes and ethyl propiolate as raw materials. The mechanisms for the formation of 1,4-DHP and 1,2-DHP were proposed based on the isolated intermediate named diethyl 4-((phenylamino)methylene)pent-2-enedioate generated by the Michael addition of aniline and ethyl propiolate. The transition state structures were optimized and the reaction energy barriers of intermediates in the speculated mechanisms were calculated by DFT calculations at the M062X/def2TZVP//B3LYP-D3/def-SVP level. It was found that the reaction energy barriers and dominant configurations of intermediates IM2 and IM3' are the determinants for the chemoselectivity. Together, these results demonstrate a high chemoselectivity in the synthesis of 1,4-DHPs and 1,2-DHPs by a Hantzsch-like reaction and that 1,4-DHPs and 1,2-DHPs can be easily obtained under different conditions.
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http://dx.doi.org/10.1039/d0ob02289fDOI Listing
May 2021

Bandwidth-control orbital-selective delocalization of 4f electrons in epitaxial Ce films.

Nat Commun 2021 May 4;12(1):2520. Epub 2021 May 4.

Center for Correlated Matter and Department of Physics, Zhejiang University, Hangzhou, China.

The 4f-electron delocalization plays a key role in the low-temperature properties of rare-earth metals and intermetallics, and it is normally realized by the Kondo coupling between 4f and conduction electrons. Due to the large Coulomb repulsion of 4f electrons, the bandwidth-control Mott-type delocalization, commonly observed in d-electron systems, is difficult in 4f-electron systems and remains elusive in spectroscopic experiments. Here we demonstrate that the bandwidth-control orbital-selective delocalization of 4f electrons can be realized in epitaxial Ce films by thermal annealing, which results in a metastable surface phase with reduced layer spacing. The quasiparticle bands exhibit large dispersion with exclusive 4f character near [Formula: see text] and extend reasonably far below the Fermi energy, which can be explained from the Mott physics. The experimental quasiparticle dispersion agrees well with density-functional theory calculation and also exhibits unusual temperature dependence, which could arise from the delicate interplay between the bandwidth-control Mott physics and the coexisting Kondo hybridization. Our work opens up the opportunity to study the interaction between two well-known localization-delocalization mechanisms in correlation physics, i.e., Kondo vs Mott, which can be important for a fundamental understanding of 4f-electron systems.
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http://dx.doi.org/10.1038/s41467-021-22710-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096960PMC
May 2021

A Multi-Echelon Network Design in a Dual-Channel Reverse Supply Chain Considering Consumer Preference.

Authors:
Peng Li Di Wu

Int J Environ Res Public Health 2021 Apr 29;18(9). Epub 2021 Apr 29.

Faculty of Economics and Management, Xi'an University of Technology, Xi'an 710048, China.

The rapid development of e-commerce technologies has encouraged collection centers to adopt online recycling channels in addition to their existing traditional (offline) recycling channels, such the idea of coexisting traditional and online recycling channels evolved a new concept of a dual-channel reverse supply chain (DRSC). The adoption of DRSC will make the system lose stability and fall into the trap of complexity. Further the consumer-related factors, such as consumer preference, service level, have also severely affected the system efficiency of DRSC. Therefore, it is necessary to help DRSCs to design their networks for maintaining competitiveness and profitability. This paper focuses on the issues of quantitative modelling for the network design of a general multi-echelon, dual-objective DRSC system. By incorporating consumer preference for the online recycling channel into the system, we investigate a mixed integer linear programming (MILP) model to design the DRSC network with uncertainty and the model is solved using the ε-constraint method to derive optimal Pareto solutions. Numerical results show that there exist positive correlations between consumer preference and total collective quantity, online recycling price and the system profits. The proposed model and solution method could assist recyclers in pricing and service decisions to achieve a balance solution for economic and environmental sustainability.
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http://dx.doi.org/10.3390/ijerph18094760DOI Listing
April 2021

Titanium dioxide nanotubes as drug carriers for infection control and osteogenesis of bone implants.

Drug Deliv Transl Res 2021 May 3. Epub 2021 May 3.

Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE), and Xi'an Institute of Biomedical Materials & Engineering (IBME), Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, 710072, China.

Titanium implants have been widely used as one of the most effective treatments of bone defects. However, the lack of osteogenesis and bacteria-resistant activities result in high infection and loosening rates of titanium implants. Anodic oxidation could easily construct titanium dioxide nanotubes (TNTs) array on the surface of titanium, and the rough surface of TNTs is beneficial to the growth of osteoblast-related cells on the surface. And TNTs could be excellent drug carriers because of their single-entry tubular hollow structure. In this review, we aim at detailing the application of TNTs as drug carriers in the field of bone implants. Starting from the topography of TNTs, we illustrated the biological activity of the TNTs surface, the drugs for loading in TNTs, and the controlled and responsive release strategies of drug-loaded TNTs, respectively. At the end of this review, the shortcomings of TNTs as the drug carrier in the field of bone implants are discussed, and the development direction of this research field is also prospected.
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http://dx.doi.org/10.1007/s13346-021-00980-zDOI Listing
May 2021

Bacillus amyloliquefaciens B10 can alleviate aflatoxin B1-induced kidney oxidative stress and apoptosis in mice.

Ecotoxicol Environ Saf 2021 Apr 29;218:112286. Epub 2021 Apr 29.

Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech Republic. Electronic address:

Aflatoxin B1(AFB1) widely exists in food and feed, which seriously endangers human and animal health. How to detoxify AFB1 is a research hotspot at present. This study attempts to use the Bacillus amyloliquefaciens B10, one of probiotics strain as the research object to ascertain whether it can alleviate the kidney injury induced by AFB1 in mice and its mechanism. Fifty-six mice were divided into four groups (control, AFB1, AFB1 + B10, and B10). The mice that received intragastric administration for 28 days were euthanised, and serum was collected for biochemical index detection with fresh kidney tissue taken for HE staining, TUNEL detection, and protein expression detection. Our results showed that the biochemical indices changed, significant pathological changes appeared, the number of apoptotic cells increased in the kidney tissue of the AFB1 group mice; the protein expressions of Nrf2, HO-1,AKT, P-AKT, and Bcl-2 in the AFB1 group were significantly decreased; the protein expressions of Keap-1, PTEN, Bax, Caspase-9, and Caspase-3 were significantly increased. After B. amyloliquefaciens B10 co-treatment, compared with the AFB1 group, the biochemical indices, pathological changes, and protein expressions were significantly reversed. The results indicated that B. amyloliquefaciens B10 can alleviate AFB1-induced kidney injury in mice.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112286DOI Listing
April 2021

Interaction of gibberellin and other hormones in almond anthers: phenotypic and physiological changes and transcriptomic reprogramming.

Hortic Res 2021 May 1;8(1):94. Epub 2021 May 1.

College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi, 830052, China.

Low temperature causes anther dysfunction, severe pollen sterility and, ultimately, major yield losses in crop plants. Previous studies have shown that the gibberellic acid (GA) metabolic pathway plays an important role in this process by regulating tapetum function and pollen development. However, the interaction mechanism of GA with other hormones mediating anther development is still unclear. Herein, we collected and analyzed almond (Amygdalus communis L.) anthers at the meiosis, tetrad, 1-nucleus, and mature 2-nucleus stages. The growth rate per 1000 anthers exhibited a significant positive correlation with the total bioactive GA compound content, and the levels of all bioactive GA compounds were highest in the 1-nucleus pollen stage. GA treatment experiments indicated that exogenous GA increased the levels of indole-3-acetic acid (IAA), trans-zeatin (tZ), and jasmonic acid (JA) and decreased the levels of salicylic acid (SA) and abscisic acid (ABA); moreover, GA improved pollen viability and quantities under cold conditions, whereas PP (paclobutrazol, an inhibitor of GA biosynthesis) was antagonistic with GA in controlling anther development. RNA-seq and qRT-PCR results showed that GA played an important role in anther development by regulating the expression of other phytohormone pathway genes, dehydration-responsive element-binding/C-repeat binding factor (DREB1/CBF)-mediated signaling genes, and anther development pathway genes. Our results reveal the novel finding that GA interacts with other hormones to balance anther development under normal- and low-temperature conditions in almond.
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http://dx.doi.org/10.1038/s41438-021-00527-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087710PMC
May 2021

The and anti-inflammatory activities of triterpene saponins from .

Nat Prod Res 2020 Oct 14:1-4. Epub 2020 Oct 14.

Fujian Provincial Key Laboratory of Natural Medicine Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou, P. R. China.

is widely used in She Ethnopharmacy in China owing to its significant anti-inflammatory activities. This study aimed to investigate the anti-inflammatory effect of the active fraction of (CFAF) in an arthritis animal model and its possible mechanism. Pre-inflammatory cytokine levels were examined by ELISA. CFAF can significantly improve the symptoms of arthritis such as paw swelling, arthritic index, and histological condition in AA rat. CFAF can also reduce levels of IL-1β, TNF-α and IL-6. Further studies showed that triterpene saponins from CFAF induced anti-inflammatory activity inhibited inflammatory mediators by blocking JAK/STAT signalling pathways in the LPS-treated macrophages.
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http://dx.doi.org/10.1080/14786419.2020.1833203DOI Listing
October 2020

Fully biodegradable polylactide foams with ultrahigh expansion ratio and heat resistance for green packaging.

Int J Biol Macromol 2021 Apr 27;183:222-234. Epub 2021 Apr 27.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering of China, Sichuan University, Chengdu 610065, PR China.

Long chain branching (LCB) structures are efficiently introduced into polylactide (PLA) by employing sustainable soybean oil (SO) under the initiation of trace amount of cyclic peroxide, which displays robust foamability and heat resistance. It is discovered that with the introduction of 0.6 wt% SO, the expansion ratio and Vicat softening temperature of LCB PLA are sharply raised to 75.2-fold and 155.8 °C, respectively, which is about 17.9 and 2.6 times those of linear PLA. This is because that the amounts of LCB structures are significantly increased in LCB PLA by the addition of SO with low reactivity of internal CC bonds, which can avoid the oligomerization reaction, resulting in more dramatically improved melting strength and crystallization performance of LCB PLA. Moreover, the hydrolytic degradation of LCB PLA is largely expedited as compared to linear PLA, owing to the more rapid water permeation caused by the loose packing of LCB structures. Finally, the PLA foam tray with light weight and good heat resistance is successfully developed by using LCB PLA with 0.6 wt% SO through extrusion foaming with supercritical carbon oxide and thermoforming techniques. Hence, this research offers a green route to produce eco-friendly light-weight and high-heat-resistance LCB-PLA foam with full biodegradability, which is an ideal alternative to the non-degradable oil-based plastics in the field of disposable packaging products.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.146DOI Listing
April 2021

Stability Analysis: Rational Design of a Ag Nanoparticle/Polymer Brush for Fabricating Cu Coating on a PET Surface.

Langmuir 2021 May 30;37(18):5673-5681. Epub 2021 Apr 30.

Guangxi Key Laboratory of Calcium Carbonate Resources Comprehensive Utilization, College of Materials and Chemical Engineering, Hezhou University, Hezhou 542899, China.

Excellent stability of a catalytic center would facilitate the prolongation of the cycle of a chemical plating bath and the reduction of environmental pollution. In this study, silane (3-aminopropyltriethoxysilane (KH550) and γ-(2,3-epoxypropoxy)propytrimethoxysilane (KH560)) was incorporated in AgNO solution to rationally prepare a Ag nanoparticle/polymer brush (Ag/PB) catalytic solution. The effects of the KH560 relative content on the Ag/PB structure and stability were studied. The epoxy group in the KH560 could react with an amino group in the KH550 through direct ring-opening reaction to form a secondary amino group and hydroxyl, which could coadsorb Ag nanoparticles by means of a chelating structure; hence, Ag/PB with superior Ag-adsorbed intensity was established on a polyethylene terephthalate (PET) surface. Ag particles on PB with 75% KH560 revealed the best stability of those measured, and the relative Ag surplus was 56.7% after stability testing. The generated Ag/PB that served as catalytic centers to catalyze the electroless copper plating resulted in a facile technology for preparing Cu/PET composite material. This means that the technology has potential application in a green process for preparing metal/polymer composite materials.
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http://dx.doi.org/10.1021/acs.langmuir.1c00567DOI Listing
May 2021

Short-Term Effect of Percutaneous Coronary Intervention on Heart Rate Variability in Patients with Coronary Artery Disease.

Entropy (Basel) 2021 Apr 28;23(5). Epub 2021 Apr 28.

Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Myocardial ischemia in patients with coronary artery disease (CAD) leads to imbalanced autonomic control that increases the risk of morbidity and mortality. To systematically examine how autonomic function responds to percutaneous coronary intervention (PCI) treatment, we analyzed data of 27 CAD patients who had admitted for PCI in this pilot study. For each patient, five-minute resting electrocardiogram (ECG) signals were collected before and after the PCI procedure. The time intervals between ECG collection and PCI were both within 24 h. To assess autonomic function, normal sinus RR intervals were extracted and were analyzed quantitatively using traditional linear time- and frequency-domain measures [i.e., standard deviation of the normal-normal intervals (SDNN), the root mean square of successive differences (RMSSD), powers of low frequency (LF) and high frequency (HF) components, LF/HF] and nonlinear entropy measures [i.e., sample entropy (SampEn), distribution entropy (DistEn), and conditional entropy (CE)], as well as graphical metrics derived from Poincaré plot [i.e., Porta's index (PI), Guzik's index (GI), slope index (SI) and area index (AI)]. Results showed that after PCI, AI and PI decreased significantly ( < 0.002 and 0.015, respectively) with effect sizes of 0.88 and 0.70 as measured by Cohen's static. These changes were independent of sex. The results suggest that graphical AI and PI metrics derived from Poincaré plot of short-term ECG may be potential for sensing the beneficial effect of PCI on cardiovascular autonomic control. Further studies with bigger sample sizes are warranted to verify these observations.
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http://dx.doi.org/10.3390/e23050540DOI Listing
April 2021

Transcriptomic analyses reveal dynamic changes of defense response in Glycyrrhiza uralensis leaves under enhanced ultraviolet-B radiation.

Plant Physiol Biochem 2021 Jun 24;163:358-366. Epub 2021 Apr 24.

School of Life Sciences, Ningxia University, Yinchuan, 750021, China; Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, 750021, China. Electronic address:

The amount of solar ultraviolet-B (UV-B) radiation reaching the Earth's surface is increasing due to stratospheric ozone dynamics and global climate change. Increased UV-B radiation poses a major threat to ecosystems. Although many studies have focused on the potential effects of enhanced UV-B radiation on plants, the dynamic changes of defense response in plants under continuous UV-B radiation remains enigmatic. In this study, we investigated the effect of UV-B radiation at 0.024 W/m on the UVR8-and reactive oxygen species (ROS-) signaling pathways, antioxidant system, and wax synthesis of G. uralensis. These parameters were investigated at different UV-B radiation stages (2 h, 6 h, 12 h, 24 h, 48 h, and 96 h). The results revealed that the uvr8 expression level was significantly repressed after 2 h of UV-B radiation, partly because G. uralensis rapidly acclimated to UV-B. Significant HO accumulation occurred after 12 h UV-B radiation, resulting in activation of the ROS signaling pathway and the antioxidant system. After 24 h of UV-B radiation, wax synthesis was enhanced alongside a decrease in the capacity of the main antioxidant system. The dynamic and ordered changes in these pathways reveal how different strategies function in G. uralensis at different times during adaption to enhanced UV-B radiation. This study will help us better understand dynamic changes of defense response in plant under enhanced UV-B radiation, further providing fundamental knowledge to develop plant resistance gene resources.
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http://dx.doi.org/10.1016/j.plaphy.2021.04.010DOI Listing
June 2021

Initiation and amplification of SnRK2 activation in abscisic acid signaling.

Nat Commun 2021 04 28;12(1):2456. Epub 2021 Apr 28.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

The phytohormone abscisic acid (ABA) is crucial for plant responses to environmental challenges. The SNF1-regulated protein kinase 2s (SnRK2s) are key components in ABA-receptor coupled core signaling, and are rapidly phosphorylated and activated by ABA. Recent studies have suggested that Raf-like protein kinases (RAFs) participate in ABA-triggered SnRK2 activation. In vitro kinase assays also suggest the existence of autophosphorylation of SnRK2s. Thus, how SnRK2 kinases are quickly activated during ABA signaling still needs to be clarified. Here, we show that both B2 and B3 RAFs directly phosphorylate SnRK2.6 in the kinase activation loop. This transphosphorylation by RAFs is essential for SnRK2 activation. The activated SnRK2s then intermolecularly trans-phosphorylate other SnRK2s that are not yet activated to amplify the response. High-order Arabidopsis mutants lacking multiple B2 and B3 RAFs show ABA hyposensitivity. Our findings reveal a unique initiation and amplification mechanism of SnRK2 activation in ABA signaling in higher plants.
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http://dx.doi.org/10.1038/s41467-021-22812-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080645PMC
April 2021

Sleep, rest-activity rhythms and aging: a complex web in Alzheimer's disease?

Neurobiol Aging 2021 Mar 2. Epub 2021 Mar 2.

Medical Biodynamics Program, Division of Sleep and Circadian Disorders, Departments of Medicine and Neurology, Brigham and Women's Hospital, Boston, MA, USA; Division of Sleep Medicine, Department of Medicine, Harvard Medical School, Boston, MA, USA; Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

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http://dx.doi.org/10.1016/j.neurobiolaging.2021.02.017DOI Listing
March 2021