Publications by authors named "Peng Li"

5,179 Publications

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Cost-effective integrated conservation and restoration priorities by trading off multiple ecosystem services.

J Environ Manage 2022 Aug 8;320:115915. Epub 2022 Aug 8.

School of Environment, Beijing Normal University, State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, China.

Conservation and restoration have long been regarded as two separate management avenues to maintain or enhance ecosystem functioning. Despite the commonalities in goals, restoration is generally considered a lower priority than conservation due to its generally greater cost, uncertainties in multiple trajectories and deals with already degraded habitats. However, when resources and opportunities for meeting conservation needs are limited, restoration could be an imperative avenue to provide additional benefits from conservation. The priority of conservation and restoration should be integrated based on an identical framework cost effectively to obtain the maximum ecological benefits with minimal costs. We propose a methodological framework to integrate conservation and restoration based on theories of Systematic Conservation Planning, which could identify best integrated conservation and restoration pattern in a cost-effective way on the basis of the provisions of multiple ecosystem services (i.e., carbon storage, water yield, soil retention and habitat quality). The trade-offs among four ecosystem services are assessed with an each of 10% increment in the target levels of ecosystem services. We demonstrated our approach at a regional scale, in the Dongting Lake Area, China. Our results showed that conservation is prioritized in a higher proportion of the study area when the targets are low. When the target level became higher, restoration gained more importance with growing area. This highlights that restoration pattern is indispensable when target setting become high and the integrated conservation and restoration planning is more cost efficient than that of conservation alone. Improving the carbon storage and soil retention would also contribute greatly to an increase in other ecosystems, but increasing the water yield and habitat quality would not guarantee an improvement for others. Integrated conservation and restoration planning will facilitate refine target achievement of conservation and restoration recommendations, by the trade-offs between conservation and restoration, and among different ecosystem services, our prioritization framework provides a useful insight in implementing the integrated planning, which can improve the efficiency in increasing ecosystem services compared to use either conservation or restoration ways.
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http://dx.doi.org/10.1016/j.jenvman.2022.115915DOI Listing
August 2022

Laparoscopic surgery of intra-abdominal lymphatic malformation in children.

Exp Ther Med 2022 Sep 19;24(3):581. Epub 2022 Jul 19.

Department of Pediatric Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

Lymphatic malformation (LM) in the abdomen is uncommon in children, and the standard treatment is surgical excision. The present study aimed to evaluate the safety and efficacy of laparoscopic surgery of intra-abdominal LM in a pediatric population. The medical records of 10 children with intra-abdominal LM treated by laparoscopic resection from March 2017 to June 2021 in The Second Affiliated Hospital of Xi'an Jiaotong University (Xi'an, China) were retrospectively reviewed. Equal numbers of female and male patients were included and underwent surgery at the median age of 55 months (range, 40 days-94 months). Abdominal pain was the most frequent symptom presenting in eight patients (80%). All children were referral patients, and half of them presented to the emergency department with acute abdominal pain. All 10 of the patients benefited from laparoscopic treatment; three patients underwent extraperitoneal segmental intestinal resection and anastomosis via an enlarged umbilical incision, and two patients were converted to laparotomy because of a large retroperitoneal LM with involvement of the colon. No recurrence or complications occurred in the patients during the follow-up period with mean of 35 months (range, 11-60 months) after surgery. Overall, laparoscopic resection of intra-abdominal LM was an effective, minimally invasive therapy in the pediatric population. Segmental intestinal resection is usually required to achieve the complete removal of lesions to lessen the risk for recurrence.
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http://dx.doi.org/10.3892/etm.2022.11519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353511PMC
September 2022

Motion-Aligned 4D-MRI Reconstruction using Higher Degree Total Variation and Locally Low-Rank Regularization.

Magn Reson Imaging 2022 Aug 5. Epub 2022 Aug 5.

School of Electronics and Information Engineering, Harbin Institute of Technology, Harbin, China. Electronic address:

Four-dimensional magnetic resonance imaging (4D-MRI) is becoming increasingly important in radiotherapy treatment planning for its ability to simultaneously provide 3D structural information and temporal profiles of the examined tissues in a non-ionizing manner. However, the relatively long acquisition time and the resulting motion artifacts severely limit the further application of 4D-MRI. In this paper, we propose a novel motion-aligned reconstruction method based on higher degree total variation and locally low-rank regularization (maHDTV-LLR) to recover 4D MR images from the highly undersampled Fourier coefficients. Specifically, we propose a two-stage reconstruction framework alternating between a motion alignment step and a regularized optimization reconstruction step. Moreover, we incorporate the 3D-HDTV and the locally low-rank penalties into a unified framework to simultaneously exploit the spatial and temporal correlation of the 4D-MRI data. A fast alternating minimization algorithm based on variable splitting is utilized to solve the optimization problem efficiently. The performance of the proposed method is demonstrated in the context of 4D cardiac and abdominal MR images reconstruction with high undersampling factors. Numerical results show that the proposed method enables accelerated 4D-MRI with improved image quality and reduced artifacts.
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http://dx.doi.org/10.1016/j.mri.2022.08.002DOI Listing
August 2022

microRNA-497-mediated Smurf2/YY1/HIF2α axis in tumor growth and metastasis of esophageal squamous cell carcinoma.

J Biochem Mol Toxicol 2022 Aug 8:e23182. Epub 2022 Aug 8.

Department of Thoracic Surgery, First Affiliated Hospital of Soochow University, Suzhou, China.

Aberrant expression of microRNA-497 (miR-497) is associated with tumor progression, but the molecular mechanisms in tumorigenesis remain largely unknown. Here, we report that miR-497 expression is downregulated in esophageal squamous cell carcinoma (ESCC) clinical samples. Consistently, upregulation of miR-497 inhibits ESCC cell malignant properties and tumor growth in vivo. Importantly, we uncovered that miR-497 upregulation suppressed ESCC cell growth and tumor growth by inhibiting Smurf2. Mechanistically, we showed that Smurf2 was a target of miR-497, and mediated YY1 expression to elevate HIF2α expression, thereby enhancing the malignancy of ESCC cells. Together, our study uncovered the role of the miR-497-mediated Smurf2/YY1/HIF2α axis in tumor growth and metastasis, which might provide potential therapeutic targets for human ESCC.
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http://dx.doi.org/10.1002/jbt.23182DOI Listing
August 2022

Targeted analysis of sugar phosphates from glycolysis pathway by phosphate methylation with liquid chromatography coupled to tandem mass spectrometry.

Anal Chim Acta 2022 Aug 17;1221:340099. Epub 2022 Jun 17.

Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Tübingen, Germany. Electronic address:

Monitoring the glycolysis pathway remains an analytical challenge as most metabolites involved are sugar phosphates. Structural similarity, instability, high polarity, and rich negative charges of sugar phosphates make LC-MS based analysis challenging. Here, we developed an improved workflow integrating uniformly C-labeled yeast metabolite extract, TiO-based enrichment, differential stable isotope labeling phosphate methylation, porous graphic carbon column, and selected reaction monitoring acquisition. Uniformly C labeled yeast metabolite extract was used as internal standards while differential stable isotope labeled sugar phosphates worked as calibrants. The established method was validated in human plasma, platelet and cultured HeLa cells. The limits of quantification ranged between 0.25 and 0.54 pmol on column. The method was adapted and its applicability tested for human platelets in which activation with collagen-related peptide (CRP) clearly showed the upregulation of some SPx metabolites. The results document that this newly established method can be successfully used to monitor glycolysis in different biological samples. As an extension, more phosphorylated and carboxylated metabolites from the central carbon metabolism (pentose phosphate cycle, TCA cycle) were tested as well. This method showed superior performance, especially for multiple phosphorylated and carboxylated metabolites. For quantitative purpose, the concept of SPx in three sets (C-analytes, U-C-IS, deuterated calibrants) has the potential to be adapted for more anionic metabolites.
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http://dx.doi.org/10.1016/j.aca.2022.340099DOI Listing
August 2022

Simultaneous measurement of six biomarkers of dichlorvos in blood by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2022 Jul 29;1208:123381. Epub 2022 Jul 29.

School of Forensic Medicine, Shanxi Medical University and and Key Laboratory of Forensic Toxicology of Ministry of Public Security, Jinzhong 030600, Shanxi, China; Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. Electronic address:

A protocol was developed, via a data-dependent high-resolution tandem mass spectrometry (ddHRMS/MS), to detect six biomarkers of dichlorvos in its metabolites and blood adducts of butyrylcholinesterase and albumin without using standard synthetic peptides. Firstly, the adducts of dimethoxy phosphonate (DMP-BChE) and the aged adducts of methoxy phosphonate (MxP-BChE) were isoloated by immunomagnetic separation (IMS), and then digested to DMP-nonapeptide and MxP-nonapeptide by pepsin. The dichlorvos and its metabolites (Trimethyl phosphate, Dimethyl phosphate) were analyzed in the supernatant of IMS treatment after protein precipitation. The precipitate was digested by pronase to phosphorylated tyrosine (DMP-Tyr), which were quantified by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry (UPLC-MS). The linearity of detector response of all biomarkers was studied in their respective ranges, and the correlation coefficients (R) were all greater than 0.9981. The limits of detection (LOD) and limits of quantification (LOQ) were 0.2-10 ng/mL and 0.5-20 ng/mL, respectively. The recoveries of the six biomarkers were 78.6-110.0 %, matrix effect were 64.1-106.8 %. Inter- and intra-assay precision had coefficients of variation of ≤13.2 % and ≤9.7 %, respectively. In addition, MxP- BChE, DMP-Tyr, TMP, DMP and DDVP were detected in the blood of three cases who died from DDVP poisoning, but DMP- BChE was not detected in all of them, which may be caused by the instability of DMP- BChE and its easy aging to form MxP-BCHE.
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http://dx.doi.org/10.1016/j.jchromb.2022.123381DOI Listing
July 2022

Clinical characteristics and therapeutic response of immunoglobulin G4-related disease: a retrospective study of 127 Chinese patients.

Orphanet J Rare Dis 2022 08 4;17(1):307. Epub 2022 Aug 4.

Liver Research Center, Beijing Friendship Hospital, Capital Medical University; Beijing Key Laboratory of Translational Medicine on Cirrhosis, National Clinical Research Center for Digestive Diseases, No.95 Yong-an Road, Xicheng District, Beijing, 100050, People's Republic of China.

Background And Aims: Immunoglobulin G4-related disease (IgG4-RD) is a multisystem fibroinflammatory condition. The aim of the present study was to characterize the clinical features and therapeutic response of patients with IgG4-RD and identify risk factors for disease relapse.

Methods: We collected baseline data of eligible patients with IgG4-RD and analyzed clinical features by interview and review of medical records. The patients who received glucocorticoids (GC) therapy with at least 3 months follow-up were used to characterize the therapeutic response and identify risk factors for relapse.

Result: Totally 127 IgG4-RD patients, including 92 males and 35 females, were enrolled in the present study. The median age of onset was 63.0 years, ranging from 23 to 86. The pancreas, bile duct and lymph nodes were the most frequently involved organs. The serum IgG4 level was elevated in 94.5% of the patients and was correlated with the number of organs involved. Patients classified as head and neck limited group were more likely to be female. Compared to Mikulicz syndrome and systemic involvement group, pancreato-hepatobiliary group had higher aminotransferase, alkaline phosphatase, gamma-glutamyl transpeptidase, bilirubin and lower IgG4 level. Mikulicz syndrome and systemic involvement group had the highest IgG4-RD RI score, IgG level. Among 92 patients who received medical therapy with at least 3 months follow-up, 76 received GC alone or in combination with immunomodulator (IM) and 16 patients did not take GC. 74 out of the 76 patients (97.3%) achieved remission, with 59 of them remained in remission and 15 of them relapsed. Whereas 16 patients did not take GC, among them, 6 patients achieved remission with one relapsed. On multivariate analysis, higher initial score of ACR/EULAR IgG4-RD Classification Criteria and GC withdrawal were independent predictors for relapse.

Conclusion: Four phenotypes of IgG4-RD showed different demographic and serological features. GC + IM therapy was safe and effective and might protect patients from relapse. The independent risk factors of relapse were GC withdrawal and higher score of ACR/EULAR IgG4-RD Classification Criteria.
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http://dx.doi.org/10.1186/s13023-022-02404-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351161PMC
August 2022

Study on the quality and myofibrillar protein structure of chicken breasts during thawing of ultrasound-assisted slightly acidic electrolyzed water (SAEW).

Ultrason Sonochem 2022 Jul 29;88:106105. Epub 2022 Jul 29.

College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China. Electronic address:

The effects of air thawing (AT), water thawing (WT), slightly acidic electrolyzed water (ET), ultrasound-assisted water thawing (WUT) and ultrasound-assisted slightly acidic electrolyzed water (EUT) on the quality and myofibrillar protein (MP) structure of chicken breasts were investigated. The results showed that WUT and EUT could significantly improve the thawing rate compared with AT, WT, and ET groups. The EUT group not only had lower thawing loss, but also their immobilized and free water contents were similar to fresh sample according to the low-field nuclear magnetic resonance (LF NMR) results. The EUT treatment had no adverse effect on the primary structure of the protein. The secondary and tertiary structures of MP were more stable in the EUT group according to Raman and fluorescence spectra. The muscle fibers microstructure from EUT group was neater and more compact compared with other thawing methods. Therefore, EUT treatment could be considered as a novel potential thawing method in the food industry.
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http://dx.doi.org/10.1016/j.ultsonch.2022.106105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352963PMC
July 2022

Translesion synthesis of apurinic/apyrimidic site analogues by Y-family DNA polymerase Dbh from .

Acta Biochim Biophys Sin (Shanghai) 2022 May;54(5):637-646

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion synthesis (TLS) activity. To investigate the effect of the molecular structure of the AP site on the TLS efficiency of Dbh, a Y-family DNA polymerase from , a series of different AP site analogues (various spacers) are used to characterize the bypass efficiency. We find that not only the molecular structure and atomic composition but also the number and position of AP site analogues determine the TLS efficiency of Dbh. Increasing the spacer length decreases TLS activity. The TLS efficiency also decreases when more than one spacer exists on the DNA template. The position of the AP site analogues is also an important factor for TLS. When the spacer is opposite to the first incorporated dNTPs, the TLS efficiency is the lowest, suggesting that AP sites are largely harmful for the formation of hydrogen bonds. These results deepen our understanding of the TLS activity of Y-family DNA polymerases and provide a biochemical basis for elucidating the TLS mechanism in cells.
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http://dx.doi.org/10.3724/abbs.2022045DOI Listing
May 2022

One-step high-yield preparation of nitrogen- and sulfur-codoped carbon dots with applications in chromium(vi) and ascorbic acid detection.

RSC Adv 2022 Jun 13;12(30):19686-19694. Epub 2022 Jul 13.

College of Food and Biological Engineering, Jimei University Xiamen 361021 P. R. China

In this research, a nitrogen- (N) and sulfur- (S) codoped carbon dot (CDs-IPM)-based sensor was synthesized using a single-step hydrothermal method. Specifically, microcrystalline cellulose (MCC) was the main raw material, which was extracted from banana pseudo-stem-based waste, while autonomous sulfonic acid-functionalized ionic liquid (SOH-IL) and polyethylene glycol 400 (PEG 400) acted as the N, S dopant, and surface modifier, respectively. Comprehensive spectroscopic characterization of the synthesized CDs-IPM revealed the introduction of S, N atoms in the matrix with existence of surface oxygenic functional groups. The CDs-IPM possessed enhanced photoluminescence (PL) intensity, synthetic yield, and PL quantum yield (PLQY). Additionally, electron transfer between the CDs-IPM, hexavalent chromium (Cr(vi)), and subsequent ascorbic acid (AA) succeeded in turning the fluorescence on and off. The detection limit was 17 nM for Cr(vi), while it was 103 nM for AA. Our study data can simplify the process of synthesis of CDs utilizing biodegradable starting materials. The probe reported in this study may serve as a valuable addition to the field of environment monitoring by virtue of its enhanced detection sensitivity, high selectivity, and stability.
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http://dx.doi.org/10.1039/d2ra01758jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277421PMC
June 2022

BET bromodomain inhibition rescues PD-1-mediated T-cell exhaustion in acute myeloid leukemia.

Cell Death Dis 2022 Aug 2;13(8):671. Epub 2022 Aug 2.

Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, Jinan University, 510632, Guangzhou, P. R. China.

Sustained expression of programmed cell death receptor-1 (PD-1) is correlated with the exhaustion of T cells, and blockade of the PD-1 pathway is an effective immunotherapeutic strategy for treating various cancers. However, response rates are limited, and many patients do not achieve durable responses. Thus, it is important to seek additional strategies that can improve anticancer immunity. Here, we report that the bromodomain and extraterminal domain (BET) inhibitor JQ1 inhibits PD-1 expression in Jurkat T cells, primary T cells, and T-cell exhaustion models. Furthermore, JQ1 dramatically impaired the expression of PD-1 and T-cell immunoglobulin mucin-domain-containing-3 (Tim-3) and promoted the secretion of cytokines in T cells from patients with acute myeloid leukemia (AML). In line with that, BET inhibitor-treated CD19-CAR T and CD123-CAR T cells have enhanced anti-leukemia potency and resistant to exhaustion. Mechanistically, BRD4 binds to the NFAT2 and PDCD1 (encoding PD-1) promoters, and NFAT2 binds to the PDCD1 and HAVCR2 (encoding Tim-3) promoters. JQ1-treated T cells showed downregulated NFAT2, PD-1, and Tim-3 expression. In addition, BET inhibitor suppressed programmed death-ligand 1 (PD-L1) expression and cell growth in AML cell lines and in primary AML cells. We also demonstrated that JQ1 treatment led to inhibition of leukemia progression, reduced T-cell PD-1/Tim-3 expression, and prolonged survival in MLL-AF9 AML mouse model and Nalm6 (B-cell acute lymphoblastic leukemia cell)-bearing mouse leukemia model. Taken together, BET inhibition improved anti-leukemia immunity by regulating PD-1/PD-L1 expression, and also directly suppressed AML cells, which provides novel insights on the multiple effects of BET inhibition for cancer therapy.
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http://dx.doi.org/10.1038/s41419-022-05123-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346138PMC
August 2022

Nanoflow Sheath Voltage-Free Interfacing of Capillary Electrophoresis and Mass Spectrometry for the Detection of Small Molecules.

Anal Chem 2022 Aug 1. Epub 2022 Aug 1.

C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, West Virginia 26505, United States.

Coupling capillary electrophoresis (CE) to mass spectrometry (MS) is a powerful strategy to leverage a high separation efficiency with structural identification. Traditional CE-MS interfacing relies upon voltage to drive this process. Additionally, sheathless interfacing requires that the electrophoresis generates a sufficient volumetric flow to sustain the ionization process. Vibrating sharp-edge spray ionization (VSSI) is a new method to interface capillary electrophoresis to mass analyzers. In contrast to traditional interfacing, VSSI is voltage-free, making it straightforward for CE and MS. New nanoflow sheath CE-VSSI-MS is introduced in this work to reduce the reliance on the separation flow rate to facilitate the transfer of analyte to the MS. The nanoflow sheath VSSI spray ionization functions from 400 to 900 nL/min. Using the new nanoflow sheath reported here, volumetric flow rate through the separation capillary is less critical, allowing the use of a small (i.e., 20 to 25 μm) inner diameter separation capillary and enabling the use of higher separation voltages and faster analysis. Moreover, the use of a nanoflow sheath enables greater flexibility in the separation conditions. The nanoflow sheath is operated using aqueous solutions in the background electrolyte and in the sheath, demonstrating the separation can be performed under normal and reversed polarity in the presence or absence of electroosmotic flow. This includes the use of a wider pH range as well. The versatility of nanoflow sheath CE-VSSI-MS is demonstrated by separating cationic, anionic, and zwitterionic molecules under a variety of separation conditions. The detection sensitivity observed with nanoflow sheath CE-VSSI-MS is comparable to that obtained with sheathless CE-VSSI-MS as well as CE-MS separations with electrospray ionization interfacing. A bare fused silica capillary is used to separate cationic β-blockers with a near-neutral background electrolyte at concentrations ranging from 1.0 nM to 1.0 μM. Under acidic conditions, 13 amino acids are separated with normal polarity at a concentration ranging from 0.25 to 5 μM. Finally, separations of anionic compounds are demonstrated using reversed polarity under conditions of suppressed electroosmotic flow through the use of a semipermanent surface coating. With a near-neutral separation electrolyte, anionic nonsteroidal anti-inflammatory drugs are detected over a concentration range of 0.1 to 5.0 μM.
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http://dx.doi.org/10.1021/acs.analchem.2c02074DOI Listing
August 2022

Salvianolic Acid A Protects against Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Neutrophil NETosis.

Oxid Med Cell Longev 2022 21;2022:7411824. Epub 2022 Jul 21.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, China.

Salvianolic acid A (SAA) is one of bioactive polyphenol extracted from a Salvia miltiorrhiza (Danshen), which was widely used to treat cardiovascular disease in traditional Chinese medicine. SAA has been reported to be protective in cardiovascular disease and ischemia injury, with anti-inflammatory and antioxidative effect, but its role in acute lung injury (ALI) is still unknown. In this study, we sought to investigate the therapeutic effects of SAA in a murine model of lipopolysaccharide- (LPS-) induced ALI. The optimal dose of SAA was determined by comparing the attenuation of lung injury score after administration of SAA at three different doses (low, 5 mg/kg; medium, 10 mg/kg; and, high 15 mg/kg). Dexamethasone (DEX) was used as a positive control for SAA. Here, we showed that the therapeutic effect of SAA (10 mg/kg) against LPS-induced pathologic injury in the lungs was comparable to DEX. SAA and DEX attenuated the increased W/D ratio and the protein level, counts of total cells and neutrophils, and cytokine levels in the BALF of ALI mice similarly. The oxidative stress was also relieved by SAA and DEX according to the superoxide dismutase and malondialdehyde. NET level in the lungs was elevated in the injured lung while SAA and DEX reduced it significantly. LPS induced phosphorylation of Src, Raf, MEK, and ERK in the lungs, which was inhibited by SAA and DEX. NET level and phosphorylation level of Src/Raf/MEK/ERK pathway in the neutrophils from acute respiratory distress syndrome (ARDS) patients were also inhibited by SAA and DEX in vitro, but the YEEI peptide reversed the protective effect of SAA completely. The inhibition of NET release by SAA was also reversed by YEEI peptide in LPS-challenged neutrophils from healthy volunteers. Our data demonstrated that SAA ameliorated ALI via attenuating inflammation, oxidative stress, and neutrophil NETosis. The mechanism of such protective effect might involve the inhibition of Src activation.
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http://dx.doi.org/10.1155/2022/7411824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334034PMC
August 2022

Optical Visualization of Red-GQDs' Organelles Distribution and Localization in Living Cells.

Front Pharmacol 2022 13;13:932807. Epub 2022 Jul 13.

Shandong Provincial Hospital, Shandong University, Jinan, China.

Recently, there has been a rapidly expanding interest in a new nanomaterial, graphene quantum dots (GQDs), owing to its profound potential in various advanced applications. At present, the study of GQDs mainly focuses on the new synthesis methods and surface modification. However, revealing the intracellular distribution of GQDs is currently not available, limiting in-depth understanding of its biological regulatory mechanism. To fill up this gap, the visualization study of red fluorescent graphene quantum dots (Red-GQDs) is helpful to clarify their subcellular distribution and metabolism in living cells system. Here, in this study, two-photon laser confocal microscopy was used to deeply analyze the uptake and subcellular distribution of Red-GQDs by HeLa cells at different concentrations and times through visual observation and discussed the effect of Red-GQDs on the metabolic of HeLa cells. The results indicated that Red-GQDs could be well-absorbed by HeLa cells and further revealed the differential distribution of Red-GQDs in different organelles (lysosomes and mitochondria) in a time-dependent manner. In addition, we confirmed that Red-GQDs significantly affect cell biological functions. Low concentrations of Red-GQDs are related to the autophagy pathway of cells, and high concentrations of Red-GQDs can induce ferroptosis in cells and promote the secretion of cellular exosomes. In the present study, the distribution and metabolic pathways of Red-GQDs in the subcellular structure of cells were characterized in detail through visual analysis, which can bring positive reference for the application of Red-GQDs in the future.
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http://dx.doi.org/10.3389/fphar.2022.932807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326348PMC
July 2022

mTORC1 coordinates NF-κB signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification.

Bone 2022 Jul 29;163:116507. Epub 2022 Jul 29.

Department of Orthopedics, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, China; Academy of Orthopaedics, Guangdong Province, Guangzhou 510630, China. Electronic address:

Heterotopic ossification (HO) is a pathological bone formation based on endochondral ossification distinguished by ossification within muscles, tendons, or other soft tissues. There has been growing studies focusing on the treatment with rapamycin to inhibit HO, but the mechanism of mTORC1 on HO remains unclear. Tendon cells (TDs) are the first cells to form during tendon heterotopic ossification. Here, we used an in vivo model of HO and an in vitro model of chondrogenesis induction to elucidate the effect and underlying mechanism of mTORC1 in HO. The current study highlights the effect of rapamycin on murine Achilles tenotomy-induced HO and the role of mTORC1 signaling pathway on TDs. Our result showed that mTORC1 was activation in the early stage of HO, whereas the mTORC1 maintained low expression in the mature ectopic cartilage tissue and the ectopic bone formation sites. The use of mTORC1-specific inhibitor (rapamycin) immediately after Achilles tendon injury could suppress the formation of HO; once ectopic cartilage and bone had formed, treatment with rapamycin could not significantly inhibit the progression of HO. Mechanistically, mTORC1 stimulation by silencing of TSC1 promoted the expression of the chondrogenic markers in TDs. In TDs, treated with mTORC1 stimulation by silencing of TSC1, mTORC1 increased the activation of the NF-κB signaling pathway. NF-κB selective inhibitor BAY11-7082 significantly suppressed the chondrogenesis of TDs that treated with mTORC1 stimulation by silencing of TSC1. Together, our findings demonstrated that mTORC1 promoted HO by regulating TDs chondrogenesis partly through the NF-κB signaling pathway; and rapamycin could be a viable HO therapeutic regimen.
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http://dx.doi.org/10.1016/j.bone.2022.116507DOI Listing
July 2022

Associations among the nursing work environment, nurse-reported workplace bullying, and patient outcomes.

Nurs Forum 2022 Jul 31. Epub 2022 Jul 31.

University of Alabama at Birmingham School of Nursing, Birmingham, Alabama, USA.

Background: Bullying may undermine patient safety in healthcare organizations threatening quality improvement and patient outcomes.

Purpose: To explore the associations between the nursing work environment, nurse-reported workplace bullying, and patient outcomes.

Method: Cross-sectional analysis of nurse survey data (N = 943). The Practice Environment Scale of the nursing work index was used to measure the work environment, nurse-reported bullying was measured with the short negative acts questionnaire, and single items measured care quality and patient safety grade. Random effects logistic regressions were used to determine associations controlling for individual, employment, and organizational factors.

Findings: Fourty percent of nurses reported experiencing bullying. A higher work environment composite score was significantly associated with a lower risk of bullying (OR = 0.16 [0.12, 0.22], p < .0001). Nurses experiencing bullying were less likely to report good/excellent quality of care (OR = 0.28 [0.18, 0.44], p < .0001) or a favorable patient safety grade (OR = 0.36 [0.25, 0.51], p < .0001).

Discussion: The nursing work environment influences the presence of bullying, which can negatively impact patient outcomes. Improving nurse work environments is one mechanism to better address nurse bullying.
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http://dx.doi.org/10.1111/nuf.12781DOI Listing
July 2022

Dysfunction of histone demethylase IBM1 in Arabidopsis causes autoimmunity and reshapes the root microbiome.

ISME J 2022 Jul 30. Epub 2022 Jul 30.

Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China.

Root microbiota is important for plant growth and fitness. Little is known about whether and how the assembly of root microbiota may be controlled by epigenetic regulation, which is crucial for gene transcription and genome stability. Here we show that dysfunction of the histone demethylase IBM1 (INCREASE IN BONSAI METHYLATION 1) in Arabidopsis thaliana substantially reshaped the root microbiota, with the majority of the significant amplicon sequence variants (ASVs) being decreased. Transcriptome analyses of plants grown in soil and in sterile growth medium jointly disclosed salicylic acid (SA)-mediated autoimmunity and production of the defense metabolite camalexin in the ibm1 mutants. Analyses of genome-wide histone modifications and DNA methylation highlighted epigenetic modifications permissive for transcription at several important defense regulators. Consistently, ibm1 mutants showed increased resistance to the pathogen Pseudomonas syringae DC3000 with stronger immune responses. In addition, ibm1 showed substantially impaired plant growth promotion in response to beneficial bacteria; the impairment was partially mimicked by exogenous application of SA to wild-type plants, and by a null mutation of AGP19 that is important for cell expansion and that is repressed with DNA hypermethylation in ibm1. IBM1-dependent epigenetic regulation imposes strong and broad impacts on plant-microbe interactions and thereby shapes the assembly of root microbiota.
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http://dx.doi.org/10.1038/s41396-022-01297-6DOI Listing
July 2022

Diversity of endophytic fungi in Coptis chinensis Franch. and their activity against methicillin-resistant Staphylococcus aureus.

Folia Microbiol (Praha) 2022 Jul 30. Epub 2022 Jul 30.

Department of Pharmacognosy, College of Pharmacy, Army Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

This study aimed at investigating the diversity of endophytic fungi from Coptis chinensis and their activity against methicillin-resistant Staphylococcus aureus (MRSA). Seventy-nine fungal isolates obtained from C. chinensis were identified to belong to 27 species based on morphological features and internal transcript spacer (ITS) gene sequencing analysis. Comparing relative frequency values, the most frequent genera were Colletotrichum and Fusarium, while most frequent species were C. gloeosporioides and F. avenaceum. Analysis of diversity indices indicated that C. chinensis harbored abundant fungal resources. Methanol extracts of fungal endophyte cultures were evaluated for antibacterial activity against S. aureus ATCC 25923 and two other MRSA clinical strains. Nine of 27 endophytic fungi exhibited inhibitory activities against S. aureus ATCC 25923. Among them, Paraboeremia litseae HL-17, Fusarium sp. HL-23, and Fusarium sp. HL-27 exhibited obvious inhibition against the three S. aureus strains. Our findings suggest that the endophytic fungi in C. chinensis have a high diversity and an obvious tissue specificity, and could be of potential interest in screening anti-MRSA agents. To the best of our knowledge, this is the first report on the diversity and anti-MRSA activity of fungal endophytes from C. chinensis.
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http://dx.doi.org/10.1007/s12223-022-00994-1DOI Listing
July 2022

High-temperature flexible WSe photodetectors with ultrahigh photoresponsivity.

Nat Commun 2022 Jul 28;13(1):4372. Epub 2022 Jul 28.

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, 100084, China.

The development of high-temperature photodetectors can be beneficial for numerous applications, such as aerospace engineering, military defence and harsh-environments robotics. However, current high-temperature photodetectors are characterized by low photoresponsivity (<10 A/W) due to the poor optical sensitivity of commonly used heat-resistant materials. Here, we report the realization of h-BN-encapsulated graphite/WSe2 photodetectors which can endure temperatures up to 700 °C in air (1000 °C in vacuum) and exhibit unconventional negative photoconductivity (NPC) at high temperatures. Operated in NPC mode, the devices show a photoresponsivity up to 2.2 × 10 A/W, which is ~5 orders of magnitude higher than that of state-of-the-art high-temperature photodetectors. Furthermore, our devices demonstrate good flexibility, making it highly adaptive to various shaped surfaces. Our approach can be extended to other 2D materials and may stimulate further developments of 2D optoelectronic devices operating in harsh environments.
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http://dx.doi.org/10.1038/s41467-022-32062-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334605PMC
July 2022

Evaluations from Superiors Matter More: Evidence from Behavior and Event-related Potentials.

J Cogn Neurosci 2022 Jul 22:1-20. Epub 2022 Jul 22.

Utah State University, Logan.

As an essential feature of social groups, social hierarchies influence how people interact in daily life. However, it is unknown how external evaluations from peers at different levels in a social hierarchy influence feedback processing. In the current study, participants were instructed to establish a social hierarchy through a novel stick drawing task. With their status in the social hierarchy manipulated to be in the middle, participants received positive or negative evaluations from superiors and inferiors separately with ERPs signals recorded. Behaviorally, participants rated their happiness highest, and most quickly after receiving positive evaluations from superiors. ERP results showed that P3 amplitude exhibited an interaction between social hierarchy and feedback valence, such that larger P3 amplitude was elicited by positive evaluations from superiors than any other condition, whereas the feedback-related negativity was only sensitive to the difference between high and low social statuses. Moreover, a generalized linear mixed model examining single-trial ERP data showed P3 amplitude correlated with happiness ratings as a function of feedback valence: a significant positive correlation in the positive feedback condition and a significant negative correlation in the negative feedback condition. P3 was also negatively correlated with RT across all conditions. These results demonstrate that brain activity associated with feedback processing predicted participants' emotion ratings and their decision time. Our study provides the first ERP evidence that positive feedback from superiors impacts neural activity related to reward processing differently than positive feedback from those of lower social standing.
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http://dx.doi.org/10.1162/jocn_a_01894DOI Listing
July 2022

Eosinophilic granulomatosis with polyangiitis with gastrointestinal symptoms as the first manifestation.

Indian J Pathol Microbiol 2022 Jul-Sep;65(3):737-738

Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing, PR China.

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http://dx.doi.org/10.4103/ijpm.ijpm_625_21DOI Listing
August 2022

Protection Induced by Oral Vaccination with a Recombinant Delivering LcrV and F1 Antigens in Mice and Rats against Pneumonic Plague.

Infect Immun 2022 Jul 28:e0016522. Epub 2022 Jul 28.

Department of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.

A newly attenuated Yersinia pseudotuberculosis strain (designated Yptb1) with triple mutation Δ Δ Δ and chromosomal insertion of the Y. pestis operon was constructed as a live vaccine platform. Yptb1 tailored with an Asd plasmid (pYA5199) (designated Yptb1[pYA5199]) simultaneously delivers Y. pestis LcrV and F1. The attenuated Yptb1(pYA5199) localized in the Peyer's patches, lung, spleen, and liver for a few weeks after oral immunization without causing any disease symptoms in immunized rodents. An oral prime-boost Yptb1(pYA5199) immunization stimulated potent antibody responses to LcrV, F1, and Y. pestis whole-cell lysate (YPL) in Swiss Webster mice and Brown Norway rats. The prime-boost Yptb1(pYA5199) immunization induced higher antigen-specific humoral and cellular immune responses in mice than a single immunization did, and it provided complete short-term and long-term protection against a high dose of intranasal Y. pestis challenge in mice. Moreover, the prime-boost immunization afforded substantial protection for Brown Norway rats against an aerosolized Y. pestis challenge. Our study highlights that Yptb1(pYA5199) has high potential as an oral vaccine candidate against pneumonic plague.
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http://dx.doi.org/10.1128/iai.00165-22DOI Listing
July 2022

Novel Findings in Teleost TRAF4, a Protein Acts as an Enhancer in TRIF and TRAF6 Mediated Antiviral and Inflammatory Signaling.

Front Immunol 2022 11;13:944528. Epub 2022 Jul 11.

Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Ornamental Aquarium Engineering Research Centre in University of Fujian Province, Fisheries College, Jimei University, Xiamen, China.

Tumor necrosis factor receptor-associated factors (TRAFs) are important adaptor molecules that play important roles in host immune regulation and inflammatory responses. Compared to other members of TRAFs, the function of TRAF4 in vertebrate immunity remains unclear, especially in teleosts. In the present study, TRAF4 ortholog was cloned and identified in large yellow croaker (), named as . The open reading frame (ORF) of - is 1,413 bp and encodes a protein of 470 amino acids (aa), which is consisted of a RING finger domain, two zinc finger domains, and a MATH domain. The genome organization of - is conserved in teleosts, amphibians, birds, and mammals, with 7 exons and 6 introns. Quantitative real-time PCR analysis revealed that - was broadly distributed in various organs/tissues of healthy large yellow croakers and could be significantly up-regulated in the gill, intestine, spleen, head kidney, and blood under poly I:C, LPS, PGN, and stimulations. Notably, luciferase assays showed that overexpression of -TRAF4 could significantly induce the activation of IRF3, IRF7, and type I IFN promoters, with the RING finger and zinc finger domains function importantly in such promoter activation. Confocal microscopy revealed that -TRAF4 is located in the cytoplasm, whereas the deletion of the RING finger, zinc finger or MATH domain showed little effect on the subcellular localization of -TRAF4. Interestingly, -TRAF4 overexpression could significantly enhance -TRIF and -TRAF6 medicated IRF3 and IRF7 promoter activation. In addition, co-expression of -TRAF4 with -TRIF or -TRAF6 could significantly induce the expression of antiviral and inflammation-related genes, including , , , , , , , and compared to the only overexpression of -TRAF4, -TRIF or -TRAF6. These results collectively imply that -TRAF4 functions as an enhancer in -TRIF and -TRAF6 mediated antiviral and inflammatory signaling.
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http://dx.doi.org/10.3389/fimmu.2022.944528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310645PMC
July 2022

Reprogramming of glutamine metabolism and its impact on immune response in the tumor microenvironment.

Cell Commun Signal 2022 07 27;20(1):114. Epub 2022 Jul 27.

Department of Urology, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao, 266003, China.

Metabolic reprogramming and immune escape play a major role in tumorigenesis. Increasing number of studies have shown that reprogramming of glutamine metabolism is a putative determinant of the anti-tumor immune response in the tumor microenvironment (TME). Usually, the predatory uptake of glutamine by tumor cells in the TME results in the limited utilization of glutamine by immune cells and affects the anti-tumor immune response. The cell-programmed glutamine partitioning also affects the anti-tumor immune response. However, the reprogramming of glutamine metabolism in tumors modulates immune escape by regulating tumor PD-L1 expression. Likewise, the reprogramming of glutamine metabolism in the immune cells also affects their immune function. Additionally, different types of glutamine metabolism inhibitors extensively regulate the immune cells in the TME while suppressing tumor cell proliferation. Herein, we discuss how metabolic reprogramming of tumor and immune cells regulates anti-tumor immune responses, as well as functional changes in different immune cells in the context of targeting tumor glutamine metabolism, which can better explain the potential of targeting glutamine metabolism in combination with immunotherapy for cancer. Video abstract.
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http://dx.doi.org/10.1186/s12964-022-00909-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327201PMC
July 2022

Low Pt-Doped Crystalline/Amorphous Heterophase PdP Nanowires as Efficient Catalysts for Methanol Oxidation.

Inorg Chem 2022 Aug 27;61(31):12466-12472. Epub 2022 Jul 27.

Department of Chemistry and Center for Atomic Engineering of Advanced Materials, School of Materials Science and Engineering, Anhui Province Key Laboratory of Chemistry for In-organic/Organic Hybrid Functionalized Materials, Anhui University, Hefei 230601, Anhui, P. R. China.

Pd-based catalysts are attractive anodic electrocatalysts for direct methanol fuel cells owing to their low cost and natural abundance. However, they suffer from sluggish reaction kinetic and insufficient electroactivity in methanol oxidation reaction (MOR). In this work, we developed a facile one-pot approach to fabricate low Pt-doped PdP nanowires with crystalline/amorphous heterophase (termed Pt-PdP NWs) for MOR. The unique crystalline/amorphous heterophase structures promote the catalytic activity by the plentiful active sites at the phase boundaries and/or interfaces and the synergistic effect between different phases. Moreover, the incorporation of trace Pt into Pd lattices modifies the electronic structure and improves the electron transfer ability. Therefore, the obtained Pt-PdP NWs display significantly enhanced electrocatalytic performance toward MOR with the mass activity of 2.35 A mg, which is 9.0, 2.9, and 2.0 times higher than those of the commercial Pd/C (0.26 A mg), PdP NWs (0.82 A mg), and commercial Pt/C (1.19 A mg). The high mass activity enables the Pt-PdP NWs to be the promising Pd-based catalysts for MOR.
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http://dx.doi.org/10.1021/acs.inorgchem.2c02055DOI Listing
August 2022

A Mid-term Follow-up Study on the Reimplantation of Autoclaved Femoral and Tibial Components as Spacers for Treating Infected Total Knee Arthroplasty.

Orthop Surg 2022 Jul 27. Epub 2022 Jul 27.

Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objective: Infection after total knee arthroplasty (TKA) is a rare but devastating complication. Different types of spaces have been used in two-stage revision. The study aimed to evaluate the effect of autoclaved femoral and tibial components as spacers for treating periprosthetic infections after TKA.

Methods: A retrospective study was performed for 13 patients (five males, eight females) with a mean age of 69 ± 6 (range, 57-80) years and suffering from periprosthetic infection after TKA. They were treated with unconventional two-stage revision from May 2008 to June 2017. In the first-stage surgery, the autoclaved femoral and tibial components were reimplanted with a new liner as a spacer after a thorough debridement. After 4-6 months, the second-stage surgery was performed according to the patients' requirements. The knee society score (KSS) and knee range of motion (ROM) were assessed before and after surgery. The reinfection rate was calculated.

Results: The mean duration of follow-up was 5.7 ± 2.1 (range, 3.1-8.8) years. Culture-positive infections comprised 69% of the cohort. All patients were able to walk 24 h after the first stage surgery, and the knee ROM could reach 90° in 1 week. Two patients (15.4%) experienced an infection recurrence. One patient was reinfected 1 year after the first stage surgery. Another patient developed reinfection 3 years after surgery but did not choose re-revision and died of pneumonia. Only one patient underwent the second stage revision. The remaining 10 patients refused to receive a new prosthesis. At the time of the final follow-up, six patients had slight pain in the knee while walking, and one patient required crutches to walk. There were no signs of prosthesis dislocation, rupture, deep vein thrombosis, pulmonary embolism, or delayed wound healing. No radiolucent lines or osteolysis were found. The mean KSS improved from 51 ± 10 (range, 35-63) points preoperatively to 79 ± 5 (range, 60-85) points at the final follow-up. The average ROM before and after the first stage surgery were 62° ± 29° (range, 10°-100°) and 104° ± 9° (range, 90°-120°) (t = 4.659, P < 0.01) respectively. The infection control rate was 84.6%.

Conclusion: Reimplantation of the autoclaved original femoral and tibial components as an articulating spacer during the first stage surgery is a valuable addition for treating an infected TKA.
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http://dx.doi.org/10.1111/os.13402DOI Listing
July 2022

Fire Behavior and Failure Model of Multilayered Wood Flour/HDPE/Polycarbonate Composites with a Sandwich Structure.

Polymers (Basel) 2022 Jul 12;14(14). Epub 2022 Jul 12.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

The flame retardancy of wood-polymer composites significantly affects their potential applications. Thus, multilayered wood flour/high-density polyethylene (HDPE)/polycarbonate (PC) composites were prepared via thermocompression to improve the fire retardancy of wood-polymer composites in this paper. Thermal degradation behavior, flame retardancy, and flexural strengths of the resulting composites were investigated using a thermogravimetric analysis, cone calorimetry, and mechanical testing machine, respectively. Results revealed that the boric acid treatment reduced the heat release rate and total heat release of the wood flour/HDPE composites and increased their mass of residues. However, boric acid reduced the flexural strength of the resulting composites. The combustion test indicated that PC cap layers suppressed the combustion of the resulting composites via the formation of carbon layers. Adding PC layers reduced heat release and increased the flexural strength of the resulting composites. Finally, the failure mode of the multilayered wood flour/HDPE/PC composites in the three-point flexural test was simulated by finite element analysis.
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http://dx.doi.org/10.3390/polym14142833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323810PMC
July 2022

Detection of Trace Explosives Using a Novel Sample Introduction and Ionization Method.

Molecules 2022 Jul 17;27(14). Epub 2022 Jul 17.

School of Electronic and Information Engineering, Soochow University, Suzhou 215006, China.

A novel sample introduction and ionization method for trace explosives detection is proposed and investigated herein, taking into consideration real-world application requirements. A thermal desorption sampling method and dielectric barrier discharge ionization (DBDI) source, with air as the discharge gas, were developed. The counter flow method was adopted firstly into the DBDI source to remove the interference of ozone and other reactive nitrogen oxides. A separated reaction region with an ion guiding electric field was developed for ionization of the sample molecules. Coupled with a homemade miniature digital linear ion trap mass spectrometer, this compact and robust design, with further optimization, has the advantages of soft ionization, a low detection limit, is free of reagent and consumable gas, and is an easy sample introduction. A range of common nitro-based explosives including TNT, 2,4-DNT, NG, RDX, PETN, and HMX has been studied. A linear response in the range of two orders of magnitude with a limit of detection (LOD) of 0.01 ng for TNT has been demonstrated. Application to the detection of real explosives and simulated mixed samples has also been explored. The work paves the path to developing next generation mass spectrometry (MS) based explosive trace detectors (ETDs).
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http://dx.doi.org/10.3390/molecules27144551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320169PMC
July 2022

Cross-Cultural Adaptation and Validation of the Fear of COVID-19 Scale for Chinese University Students: A Cross-Sectional Study.

Int J Environ Res Public Health 2022 Jul 15;19(14). Epub 2022 Jul 15.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.

Background: fear of COVID-19 is widespread among the population, especially among college students because of their increased exposure to the media information overload of the COVID-19 outbreak. The Fear of COVID-19 scale (FCV-19 S) is a relatively short instrument used to evaluate fears surrounding the COVID-19 pandemic. However, the validity and reliability of the Fear of COVID-19 Scale have not been fully investigated in Chinese university student groups.

Objectives: this study assessed the cross-cultural adaptability and reliability of the FCV-19S for Chinese university students.

Methods: a Chinese version of Fear of COVID-19 Scale (C-FCV-19S) was generated using the translation-backward translation method. Psychometric properties of the C-FCV-19S, including internal consistency, split-half reliability, construct reliability, convergent validity, and diagnostic accuracy, were evaluated. The Patient Health Questionnaire (PHQ) and Generalized Anxiety Disorder Questionnaire (GAD-7) scales were also used to evaluate participants for depression and anxiety.

Results: the C-FCV-19S has acceptable internal consistency (Cronbach's alpha: 0.872) and satisfactory split-half reliability (correlation coefficient: 0.799). Using the exploratory factor analysis (EFA), we examined the construct reliability (KMO = 0.920). The confirmatory factor analysis (CFA) confirmed that the bifactor model of scale (including general factor, factor1: the awareness of COVID-19 and physiological arousal, factor 2: fear-related thinking) had a good fit index (χ2/df =6.18, RMSEA= 0.067, SRMR = 0.028, GFI = 0.986, TLI = 0.970 and CFI= 0.988). Using depression-positive and anxiety-positive scores as reference criteria, we found that the areas under the curve were 0.70 and 0.68, respectively, and that the optimal cutoff scores of the C-FCV-19S was 17.5 (sensitivity: 66.3% and 58.7%, respectively).

Conclusions: the validity and reliability of C-FCV-19S are satisfactory, and the optimal cutoff point was 17.5. The C-FCV-19S can be applied adopted in Chinese university students.
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http://dx.doi.org/10.3390/ijerph19148624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320396PMC
July 2022
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