Publications by authors named "Peng Guo"

656 Publications

Cobalt-Catalyzed Divergent Aminofluorination and Diamination of Styrenes with -Fluorosulfonamides.

Org Lett 2021 May 10. Epub 2021 May 10.

Key Laboratory of Molecule Synthesis and Function Discovery (Fujian Province University), College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, People's Republic of China.

A cobalt-catalyzed aminofluorination reaction of styrenes with -fluorosulfonamides serving as both the amination and fluorination agents has been developed. The switch of selectivity in this catalytic reaction from aminofluorination to diamination could be easily achieved by the addition of 1.0 equiv of PPh. Both transformations tolerated a wide array of substrates under mild reaction conditions.
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http://dx.doi.org/10.1021/acs.orglett.1c01308DOI Listing
May 2021

Adsorption of Hexavalent Chromium by Sodium Alginate Fiber Biochar Loaded with Lanthanum.

Materials (Basel) 2021 Apr 26;14(9). Epub 2021 Apr 26.

College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.

Lanthanun oxide (LaO) is a lanthanum chemical compound incorporates a sensible anionic complexing ability; however, it lacks stability at a low pH scale. Biochar fibers will give the benefit of their massive space and plethoric uses on the surface to support a metal chemical compound. Herein, wet spinning technology was used to load La onto sodium alginate fiber, and to convert La into LaO through carbonization. The LaO-modified biochar (La-BC) fiber was characterized by SEM, XRD and XPS, etc. An adsorption experiment proved that La-BC showed an excellent adsorption capacity for chromates, and its saturation adsorption capacity was about 104.9 mg/g. The information suggested that the adsorption was in step with both the Langmuir and Freundlich models, following pseudo-second-order surface assimilation mechanics, which showed that the Cr (VI) adsorption was characterized by single-phase and polyphase adsorption, mainly chemical adsorption. The thermodynamic parameters proved that the adsorption process was spontaneous and endothermic. The mechanistic investigation revealed that the mechanism of the adsorption of Cr (VI) by La-BC may include electrostatic interaction, ligand exchange, or complexation. Moreover, the co-existing anions and regeneration experiments proved that the La-BC is recyclable and has good prospects in the field of chrome-containing wastewater removal.
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http://dx.doi.org/10.3390/ma14092224DOI Listing
April 2021

Two new species and a new country record of the genus emAchalinus/em (Reptilia: Squamata: Xenodermidae) from China.

Zootaxa 2021 Mar 31;4950(3):zootaxa.4950.3.6. Epub 2021 Mar 31.

State Key Laboratory of Genetic Resources and Evolution State, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China Kunming College of Life Science, University of the Chinese Academy of Sciences, Kunming Yunnan 650204, China.

Combining the results from morphological and molecular analyses, we explore the taxonomy of the genus Achalinus from Southwest China. As a result, we describe two new species, A. panzhihuaensis sp. nov. and A. yangdatongi sp. nov. from southern Sichuan and southern Yunnan provinces, respectively, and we record a new country record, A. emilyae, from Guangxi Zhuang A. R.. The mitochondrial genealogy suggests that A. panzhihuaensis sp. nov. is sister to A. meiguensis, while A. yangdatongi sp. nov. clusters with the sister species A. juliani and A. ater. Both new species show considerable genetic divergence from their recognized congeners (uncorrected p-distance 6.2 % in COI gene). Furthermore, both new species can be diagnosed from closely related congeners by a combination of pholidosis characters. With our discovery, we provide a revised key to the 13 species from China and discuss some of the remaining issues regarding the taxonomy of the genus in China.
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http://dx.doi.org/10.11646/zootaxa.4950.3.6DOI Listing
March 2021

Safety and efficacy of side-to-end anastomosis versus colonic J-pouch anastomosis in sphincter-preserving resections: an updated meta-analysis of randomized controlled trials.

World J Surg Oncol 2021 Apr 21;19(1):130. Epub 2021 Apr 21.

Department of Gastrointestinal Surgery, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Background: The application of side-to-end anastomosis (SEA) in sphincter-preserving resection (SPR) is controversial. We performed a meta-analysis to compare the safety and efficacy of SEA with colonic J-pouch (CJP) anastomosis, which had been proven effective in improving postoperative bowel function.

Methods: The protocol was registered in PROSPERO under number CRD42020206764. PubMed, Embase, Web of Science, and the Cochrane Register of Controlled Trials databases were searched. The inclusion criteria were randomized controlled trials (RCTs) that evaluated the safety or efficacy of SEA in comparison with CJP anastomosis. The outcomes included the pooled risk ratio (RR) for dichotomous variables and weighted mean differences (WMDs) for continuous variables. All outcomes were calculated with 95% confidence intervals (CI) by STATA software (Stata 14, Stata Corporation, TX, USA).

Results: A total of 864 patients from 10 RCTs were included in the meta-analysis. Patients undergoing SEA had a higher defecation frequency at 12 months after SPR (WMD = 0.20; 95% CI, 0.14-0.26; P < 0.01) than those undergoing CJP anastomosis with low heterogeneity (I = 0%, P = 0.54) and a lower incidence of incomplete defecation at 3 months after surgery (RR = 0.28; 95% CI, 0.09-0.86; P = 0.03). A shorter operating time (WMD = - 17.65; 95% CI, - 23.28 to - 12.02; P < 0.01) was also observed in the SEA group without significant heterogeneity (I = 0%, P = 0.54). A higher anorectal resting pressure (WMD = 6.25; 95% CI, 0.17-12.32; P = 0.04) was found in the SEA group but the heterogeneity was high (I = 84.5%, P = 0.84). No significant differences were observed between the groups in terms of efficacy outcomes including defecation frequency, the incidence of urgency, incomplete defecation, the use of pads, enema, medications, anorectal squeeze pressure and maximum rectal volume, or safety outcomes including operating time, blood loss, the use of protective stoma, postoperative complications, clinical outcomes, and oncological outcomes.

Conclusions: The present evidence suggests that SEA is an effective anastomotic strategy to achieve similar postoperative bowel function without increasing the risk of complications compared with CJP anastomosis. The advantages of SEA include a shorter operating time, a lower incidence of incomplete defecation at 3 months after surgery, and better sphincter function. However, close attention should be paid to the long-term defecation frequency after SPR.
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http://dx.doi.org/10.1186/s12957-021-02243-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061176PMC
April 2021

[Corrigendum] GINS2 is a novel prognostic biomarker and promotes tumor progression in early‑stage cervical cancer.

Oncol Rep 2021 May 22;45(5). Epub 2021 Apr 22.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, P.R. China.

Subsequently to the publication of the above article, the authors have realized that Figs. 2 and 6 each contained an incorrectly assembled data panel: Essentially, a couple of the data panels had been inadvertently selected twice for these figures. The corrected versions of Figs 2 and 6, including the correct data for the H&E staining of the tissue of patient 4 in Fig. 2C and the invaded cells of RNAi#2 of the Siha cell line in Fig. 6C, are shown below and on the next page. All the authors agree to the publication of this Corrigendum, and they thank the Editor of for allowing them the opportunity to publish it. Furthermore, they apologize to the readership for any inconvenience caused. [The original article was published in  37: 2652‑2662, 2017; DOI: 10.3892/or.2017.5573].
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http://dx.doi.org/10.3892/or.2021.8016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020209PMC
May 2021

Correction: A Novel Remote Follow-Up Tool Based on an Instant Messaging/Social Media App for the Management of Patients With Low Anterior Resection Syndrome: Pilot Prospective Self-Control Study.

JMIR Mhealth Uhealth 2021 Apr 10;9(4):e29325. Epub 2021 Apr 10.

Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing, China.

[This corrects the article DOI: 10.2196/22647.].
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http://dx.doi.org/10.2196/29325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094023PMC
April 2021

Long non-coding RNA HOTAIR promotes the progression of synovial sarcoma through microRNA-126/stromal cell-derived factor-1 regulation.

Oncol Lett 2021 Jun 6;21(6):444. Epub 2021 Apr 6.

Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.

The long non-coding RNA (lncRNA) HOTAIR is an oncogene, that has been reported to be aberrantly expressed in multiple types of malignant tumor tissues. However, its expression and association with synovial sarcoma (SS) remains unclear. The present study aimed to elucidate the expression level of HOTAIR in SS tissues and also identify its role. Reverse transcription-quantitative PCR was used to detect the expression level of HOTAIR and microRNA (miR)-126 in 54 tissue samples from patients with SS, in 10 tissue samples from synovium tissues of normal patients, and in SW982 cells. The protein expression level was measured using western blot analysis and cellular immunofluorescence. Cellular proliferation, invasion and migration were assessed using MTT, Transwell and wound healing assays, respectively. HOTAIR was expressed at high levels in SS tissues. In contrast, miR-126 was expressed at low levels in SS tissues, and was negatively correlated with HOTAIR expression. HOTAIR knockdown in SW982 cells inhibited cellular proliferation , but also significantly increased the ratio of cells in the G/G phase of the cell cycle, and decreased the ratio of cells in the G/S phase. In addition, HOTAIR knockdown inhibited the invasion and migration of the SW982 cells, as observed in the Transwell and wound healing assays. Furthermore, HOTAIR knockdown increased miR-126 expression level and decreased the expression level of stromal cell-derived factor-1 (SDF-1) at the protein level. On the other hand, while miR-126-mimic decreased the protein expression level of SDF-1, miR-126-inhibitor increased its expression level in SW982 cells. Notably, HOTAIR knockdown or SDF-1 knockout significantly decreased the protein expression levels of CDK1, CDK2, cyclin D1, MMP-9, vimentin and N-cadherin, and significantly increased the protein expression levels of p21, p53 and E-cadherin in SW982 cells. HOTAIR was highly expressed in SS tissues, wherein it could promote the proliferation, invasion and migration of SS cells by increasing the expression of SDF-1 via miR-126 inhibition.
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http://dx.doi.org/10.3892/ol.2021.12705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045177PMC
June 2021

Hydrochemical characterization and quality assessment of groundwater in the hilly area of the Taihang Mountains in Henan Province, China.

Environ Sci Pollut Res Int 2021 Apr 10. Epub 2021 Apr 10.

Henan Institute of Geological Environment Monitoring, Zhengzhou, 450016, China.

This study evaluated the quality of groundwater and its suitability for drinking and irrigation in the hilly area of the Taihang Mountains in Henan Province, China. Groundwater samples were collected from 43 unconfined and 20 confined wells and analyzed. The pollution index of groundwater (PIG) was estimated based on the physicochemical parameters, and seven indices, including the sodium adsorption ratio (SAR), sodium percentage (%Na), residual sodium carbonate (RSC), permeability index (PI), magnesium ratio (MR), Kelley's ratio (KR), and corrosivity ratio (CR), were calculated to qualify the groundwater within the research area for irrigation activities. Multivariate statistical techniques were performed to better understand the hydrochemical processes. Chemical analysis showed that the dominant cation and anion were Ca and HCO, respectively, and the principal hydrochemical facies was Ca-Mg-HCO. In terms of pH, total dissolved solids, Na, Cl, F, and SO, most samples were well within the limits prescribed by Chinese standards for drinking water quality, but more than half of the unconfined samples exceeded the specified limits for total hardness and nitrate. The PIG values suggested the pollution level was insignificant for all confined water samples and 72.09% of unconfined water samples, but the PIG distribution map showed that the water in the south central part of the study area had low to moderate pollution. According to the computed values of SAR, %Na, RSC, PI, KR, and MR and the results of a salinity diagram, the results further indicated that most of the studied samples were appropriate for irrigation usage. Only the CR values rendered 41.86% of the unconfined samples and 20% of the confined samples unfit for irrigation. Hence, proper measures are needed to resolve the corrosivity problem. Factor analysis resulted in the extraction of 3 factors that explained 81% of the data variability, and the extracted factors pointed towards geogenic factors governing the groundwater quality.
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http://dx.doi.org/10.1007/s11356-021-13579-1DOI Listing
April 2021

Broadly protective CD8 T cell immunity to highly conserved epitopes elicited by heat shock protein gp96-adjuvanted influenza monovalent split vaccine.

J Virol 2021 Apr 7. Epub 2021 Apr 7.

Key Laboratory of Pathogenic Microbiology and Immunology, Center for Biosafety Mega-Science, Chinese Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China

Currently, immunization with inactivated influenza virus vaccines is the most prevalent method to prevent infections. However, licensed influenza vaccines provide only strain-specific protection and need to be updated and administered yearly; thus, new vaccines that provide broad protection against multiple influenza subtypes are required. In this study, we demonstrated that intradermal immunization with gp96-adjuvanted seasonal influenza monovalent H1N1 split vaccine could induce cross-protection against both group 1 and group 2 influenza A viruses in BALB/c mice models. Vaccination in the presence of gp96 induced an apparently stronger antigen-specific T cell response than split vaccine alone. Immunization with the gp96-adjuvanted vaccine also elicited apparent cross-reactive CD8 T cell response that targeted the conserved epitopes across different influenza virus strains. These cross-reactive CD8 T cells might be recalled from a pool of memory cells established after vaccination and recruited from extra-pulmonary sites to facilitate viral clearance. Of note, six highly conserved CD8 T epitopes from the viral structural proteins HA, M1, NP and PB1 were identified to play a synergistic role in gp96-mediated cross-protection. Comparative analysis showed that most of conservative epitope-specific CTLs apparently induced by heterologous virus infection were also activated by gp96-adjuvanted vaccine, thus resulting in broader protective CD8 T cell responses. Our results demonstrated the advantage of adding gp96 to an existing seasonal influenza vaccine to improve its ability to provide better cross-protection.Owing to continuous mutations in hemagglutinin (HA) or neuraminidase (NA) or recombination of the gene segments between different strains, influenza viruses can escape the immune responses developed by vaccination. Thus, new strategies aimed to efficiently activate immune response that targets to conserved regions among different influenza viruses are urgently needed in designing broad-spectrum influenza vaccine. Heat shock protein gp96 is currently the only natural T cell adjuvant with special ability to cross-present coupled antigen to MHC I molecule and activate downstream antigen-specific CTLs response. In this study we demonstrated the advantages of adding gp96 to monovalent split influenza virus vaccine to improve its ability to provide cross-protection in BALB/c mice model and proved that gp96 activated cross-reactive CTL response is indispensable in our vaccine strategy. Due to its unique adjuvant properties, gp96 might be a promising adjuvant for designing new broad-spectrum influenza vaccines.
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http://dx.doi.org/10.1128/JVI.00507-21DOI Listing
April 2021

Network Visualization and Pyramidal Feature Comparison for Ablative Treatability Classification Using Digitized Cervix Images.

J Clin Med 2021 Mar 1;10(5). Epub 2021 Mar 1.

Communications Engineering Branch, Lister Hill National Center for Biomedical Communications, U.S. National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

Uterine cervical cancer is a leading cause of women's mortality worldwide. Cervical tissue ablation is an effective surgical excision of high grade lesions that are determined to be precancerous. Our prior work on the Automated Visual Examination (AVE) method demonstrated a highly effective technique to analyze digital images of the cervix for identifying precancer. Next step would be to determine if she is treatable using ablation. However, not all women are eligible for the therapy due to cervical characteristics. We present a machine learning algorithm that uses a deep learning object detection architecture to determine if a cervix is eligible for ablative treatment based on visual characteristics presented in the image. The algorithm builds on the well-known RetinaNet architecture to derive a simpler and novel architecture in which the last convolutional layer is constructed by upsampling and concatenating specific RetinaNet pretrained layers, followed by an output module consisting of a Global Average Pooling (GAP) layer and a fully connected layer. To explain the recommendation of the deep learning algorithm and determine if it is consistent with lesion presentation on the cervical anatomy, we visualize classification results using two techniques: our (i) Class-selective Relevance Map (CRM), which has been reported earlier, and (ii) Class Activation Map (CAM). The class prediction heatmaps are evaluated by a gynecologic oncologist with more than 20 years of experience. Based on our observation and the expert's opinion, the customized architecture not only outperforms the baseline RetinaNet network in treatability classification, but also provides insights about the features and regions considered significant by the network toward explaining reasons for treatment recommendation. Furthermore, by investigating the heatmaps on Gaussian-blurred images that serve as surrogates for out-of-focus cervical pictures we demonstrate the effect of image quality degradation on cervical treatability classification and underscoring the need for using images with good visual quality.
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http://dx.doi.org/10.3390/jcm10050953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957626PMC
March 2021

Geometric layout optimization of a large aperture thin elliptical mirror's axial and lateral support.

Appl Opt 2021 Apr;60(10):2861-2869

For passive support of large aperture telescopes, geometric layout optimization of the support structure is one of the most critical tasks because it determines the deformation of the mirror under gravity, which affects the wavefront aberration and image quality of the system. Due to a lack of symmetry, the optimization of an elliptical mirror support can be much more complex compared with circular mirrors. We optimize the geometric layout of axial and lateral support for the tertiary mirror of the Thirty Meter Telescope (TMT). Based on a theoretical analysis of the whiffletree principle, a parametric model of axial support is established based on the multi-point constraint equation. The mirror deformation SlopeRMS of the tertiary mirror under vertical gravity is used as the optimization target of the support points. The axial support point position is optimized by means of a simulated annealing algorithm and a mirror-deformed post-processing script written in Python. The TMT tertiary mirror lateral support also uses the whiffletree structure, and its in-plane layout affects the system's resonant modal frequency and the maximum load at each point. According to the dynamic equation and the static principle, the lateral support optimization model is established. The first-order resonant frequency and maximum load of the support point are the objective function. Through optimization of the axial and lateral support, the overall mirror distortion of the system is improved.
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http://dx.doi.org/10.1364/AO.405638DOI Listing
April 2021

Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration.

Bioact Mater 2021 Oct 9;6(10):3097-3108. Epub 2021 Mar 9.

Innovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, Department of Orthopaedic Surgery, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, 518107, China.

It has been proven that the mechanical microenvironment can impact the differentiation of mesenchymal stem cells (MSCs). However, the effect of mechanical stimuli in biofabricating hydroxyapatite scaffolds on the inflammatory response of MSCs remains unclear. This study aimed to investigate the effect of mechanical loading on the inflammatory response of MSCs seeded on scaffolds. Cyclic mechanical loading was applied to biofabricate the cell-scaffold composite for 15 min/day over 7, 14, or 21 days. At the predetermined time points, culture supernatant was collected for inflammatory mediator detection, and gene expression was analyzed by qRT-PCR. The results showed that the expression of inflammatory mediators ( and IL8) was downregulated ( < 0.05) and the expression of ( < 0.01) and ( < 0.05) was upregulated under mechanical loading. The cell-scaffold composites biofabricated with or without mechanical loading were freeze-dried to prepare extracellular matrix-based scaffolds (ECM-based scaffolds). Murine macrophages were seeded on the ECM-based scaffolds to evaluate their polarization. The ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying enhanced the expression of M2 polarization-related biomarkers ( and ,  < 0.05) of macrophages in vitro and increased bone volume/total volume ratio in vivo. Overall, these findings demonstrated that mechanical loading could dually modulate the inflammatory responses and osteogenic differentiation of MSCs. Besides, the ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying facilitated the M2 polarization of macrophages in vitro and bone regeneration in vivo. Mechanical loading may be a promising biofabrication strategy for bone biomaterials.
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http://dx.doi.org/10.1016/j.bioactmat.2021.02.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960680PMC
October 2021

A Novel Remote Follow-Up Tool Based on an Instant Messaging/Social Media App for the Management of Patients With Low Anterior Resection Syndrome: Pilot Prospective Self-Control Study.

JMIR Mhealth Uhealth 2021 03 19;9(3):e22647. Epub 2021 Mar 19.

Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing, China.

Background: Low anterior resection syndrome (LARS) is a common functional disorder that develops after patients with rectal cancer undergo anal preservation surgery. Common approaches to assess the symptoms of patients with LARS are often complex and time-consuming. Instant messaging/social media has great application potential in LARS follow-up, but has been underdeveloped.

Objective: The aim of this study was to compare data between a novel instant messaging/social media follow-up system and a telephone interview in patients with LARS and to analyze the consistency of the instant messaging/social media platform.

Methods: Patients with R0 resectable rectal cancer who accepted several defecation function visits via the instant messaging/social media platform and agreed to a telephone interview after the operation using the same questionnaire including subjective questions and LARS scores were included. Differences between the 2 methods were analyzed in pairs and the diagnostic consistency of instant messaging/social media was calculated based on telephone interview results.

Results: In total, 21 questionnaires from 15 patients were included. The positive rates of defecation dissatisfaction, life restriction, and medication use were 10/21 (48%), 11/21 (52%), and 8/21 (38%) for telephone interview and 10/21 (48%), 13/21 (62%), and 5/21 (24%) for instant messaging/social media, respectively. No statistically significant difference was observed between instant messaging/social media and telephone interview in terms of total LARS score (mean 22.4 [SD 11.9] vs mean 24.7 [SD 10.7], P<.21) and LARS categories (Z=-0.264, P=.79); however, instant messaging/social media showed a more negative tendency. The kappa values of 3 subjective questions were 0.618, 0.430, and 0.674, respectively. The total LARS scores were consistent between both groups (Pearson coefficient 0.760, P<.001; category correlation coefficient 0.570, P=.005). Patients with major LARS had highly consistent results, with sensitivity, specificity, kappa value, and P value of 77.8%, 91.7%, 0.704, and .001, respectively.

Conclusions: Instant messaging/social media can be a major LARS screening method. However, further research on information accuracy and user acceptance is needed before implementing a mature system.

Trial Registration: ClinicalTrials.gov NCT03009747; https://clinicaltrials.gov/ct2/show/NCT03009747.
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http://dx.doi.org/10.2196/22647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078008PMC
March 2021

Electromagnetic piezoelectric hybrid driven multi-DOF motor contact model considering drive and load state.

Sci Prog 2021 Jan-Mar;104(1):368504211002356

School of Electrical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.

This paper proposes a new research method in view of the rare research on the contact state of hybrid drive multi-degree-of-freedom motor. Firstly, the basic principle and structure of hybrid drive motor are introduced. The air gap magnetic field model and electromagnetic torque model of permanent magnet rotor are obtained by analytical method. Static analysis of piezoelectric stators are carried out by stress-strain relationship. According to the analytical method and the spatial geometric relationship, the piezoelectric driving torque under different driving conditions is obtained. Introducing Hertz contact theory and Mindlin theory, combined with piezoelectric driving torque, electromagnetic driving torque and load torque to analyze the friction situation. A friction interface model considering the dynamic nonlinearity of friction coefficient and the nonlinear change of friction force distribution is established. Finally, using Matlab software, the friction distribution diagram of the contact surface of the hybrid drive three-degree-of-freedom motor under different driving and motion states is obtained. The analysis results verify the rationality of the electromagnetic piezoelectric hybrid driven three-degree-of-freedom motor. It shows that the contact state of the three sets of piezoelectric stators are determined according to the driving condition and load, the relative speed of the piezoelectric stators and the spherical rotor is proportional to the frictional force. It provides an idea for the research of contact state of hybrid drive multi-degree-of-freedom motor, and also lays a basis for further optimizing the friction interface of this kind of motors or actuators.
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http://dx.doi.org/10.1177/00368504211002356DOI Listing
March 2021

Identification of an immune-based mRNA-lncRNA signature for overall survival in cervical squamous cell carcinoma.

Future Oncol 2021 Mar 16. Epub 2021 Mar 16.

Department of Obstetrics & Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, PR China.

To better predict the survival of cervical squamous cell carcinoma (CESC) patients, we aimed to construct a signature according to different immune infiltration. We downloaded the RNA sequences of CESC patients from the Cancer Genome Atlas database. By using single-sample gene set enrichment analysis, we separated the samples into high- and low-immunity groups. Then we separated the samples into training and testing datasets and performed the following analyses: univariate, least absolute shrinkage and selection operator analysis, multivariate Cox regression analyses and weighted gene coexpression network analysis using R software. Gene ontology and Kyoto Encyclopedia of Genes and Genomes studies were performed using the Database for Annotation, Visualization and Integrated Discovery website. We finally identified a signature with three mRNAs and two lncRNAs: , , ,  and . In short, our study constructed an mRNA-lncRNA signature related to immune infiltration to better predict the survival of CESC patients.
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http://dx.doi.org/10.2217/fon-2020-1153DOI Listing
March 2021

A Marine Antibiotic Kills Multidrug-Resistant Bacteria without Detectable High-Level Resistance.

ACS Infect Dis 2021 04 3;7(4):884-893. Epub 2021 Mar 3.

State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China.

Antibiotic resistance nowadays is spreading much faster than the introduction of new antibiotics into clinical practice. There is an urgent need for potential compounds to combat multidrug-resistant (MDR) bacteria. Marine fungi provide a promising source for chemical diversity with antibiotic-like molecules. To identify structurally distinct compounds that effectively eradicate MDR pathogens and to control the development of antibiotic resistance, we have reinvestigated equisetin, a previously reported meroterpenoid isolated from a marine sponge-derived fungus. Equisetin exerted efficient antibacterial activities against either MRSA or VRE without detectable high-level resistance. Meanwhile, equisetin, as an antibiotic adjuvant, restores colistin susceptibility to colistin-resistant bacteria toward diverse Gram-negative pathogens. Intriguingly, the low-level equisetin-resistant displayed collateral sensitivity to multiple classes of existing antibiotics with decreased capacity to produce biofilm. Lastly, equisetin showed efficacy with MRSA in three infected animal models. This work suggests that equisetin derived from marine natural products is a promising lead to overcome antibiotic resistance, providing new insight in future antibiotic discovery and development.
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http://dx.doi.org/10.1021/acsinfecdis.0c00913DOI Listing
April 2021

Iron-catalyzed tandem oxidative coupling and acetal hydrolysis reaction to prepare formylated benzothiazoles and isoquinolines.

Chem Commun (Camb) 2021 Apr 2;57(26):3271-3274. Epub 2021 Mar 2.

College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, Jiangxi 330022, P. R. China.

The aldehyde group is one of the most versatile intermediates in synthetic chemistry, and the introduction of an aldehyde group into heteroarenes is important for the transformation of molecular structure. Herein, we achieved the direct formylation of benzothiazo/les and isoquinolines. The reaction features a novel iron-catalyzed Minisci-type oxidative coupling process using commercially available 1,3-dioxolane as a formylated reagent followed by acetal hydrolysis without a separation process. The reaction can be performed under exceedingly mild reaction conditions and exhibits broad functional group tolerance.
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http://dx.doi.org/10.1039/d1cc00621eDOI Listing
April 2021

Bioinformatics analysis and verification of gene targets for renal clear cell carcinoma.

Comput Biol Chem 2021 Feb 9;92:107453. Epub 2021 Feb 9.

Department of Immuno-oncology, The Fourth Hospital of Hebei Medical University, No.12 Jiankang Road Shijiazhuang, 050011, Hebei Province, China. Electronic address:

Background: It is estimated that there are 338,000 new renal-cell carcinoma releases every year in the world. Renal cell carcinoma (RCC) is a heterogeneous tumor, of which more than 70% is clear cell renal cell carcinoma (ccRCC). It is estimated that about 30% of new renal-cell carcinoma patients have metastases at the time of diagnosis. However, the pathogenesis of renal clear cell carcinoma has not been elucidated. Therefore, it is necessary to further study the pathogenesis of ccRCC.

Methods: Two expression profiling datasets (GSE68417, GSE71963) were downloaded from the GEO database. Differentially expressed genes (DEGs) between ccRCC and normal tissue samples were identified by GEO2R. Functional enrichment analysis was made by the DAVID tool. Protein-protein interaction (PPI) network was constructed. The hub genes were excavated. The clustering analysis of expression level of hub genes was performed by UCSC (University of California Santa Cruz) Xena database. The hub gene on overall survival rate (OS) in patients with ccRCC was performed by Kaplan-Meier Plotter. Finally, we used the ccRCC renal tissue samples to verify the hub genes.

Results: 1182 common DEGs between the two datasets were identified. The results of GO and KEGG analysis revealed that variations in were predominantly enriched in intracellular signaling cascade, oxidation reduction, intrinsic to membrane, integral to membrane, nucleoside binding, purine nucleoside binding, pathways in cancer, focal adhesion, cell adhesion molecules. 10 hub genes ITGAX, CD86, LY86, TLR2, TYROBP, FCGR2A, FCGR2B, PTPRC, ITGB2, ITGAM were identified. FCGR2B and TYROBP were negatively correlated with the overall survival rate in patients with ccRCC (P < 0.05). RT-qPCR analysis showed that the relative expression levels of CD86, FCGR2A, FCGR2B, TYROBP, LY86, and TLR2 were significantly higher in ccRCC samples, compared with the adjacent renal tissue groups.

Conclusions: In summary, bioinformatics technology could be a useful tool to predict the progression of ccRCC. In addition, there are DEGs between ccRCC tumor tissue and normal renal tissue, and these DEGs might be considered as biomarkers for ccRCC.
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http://dx.doi.org/10.1016/j.compbiolchem.2021.107453DOI Listing
February 2021

The Relationship Between Retinal Nerve Fiber Layer Thickness and Clinical Symptoms of Alzheimer's Disease.

Front Aging Neurosci 2020 29;12:584244. Epub 2021 Jan 29.

Center for Cognitive Neurology, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Retinal nerve fiber layer (RNFL) thickness (RT), which can reflect the status of the retinal optic nerve cells, may be affected in patients with Alzheimer's disease (AD). There are few studies on the correlation of RT of patients with AD (AD-RT) with clinical symptoms of various cognitive domains, neuropsychiatric symptoms, and activities of daily living (ADL). This study is to investigate the relationships between RT and the abovementioned clinical symptoms of AD. A total of 96 patients with AD were included in this study. RT was measured in these patients using optical coherence tomography (OCT). Demographic variables, RT, and clinical symptoms were compared between the normal and the abnormal AD-RT groups. Clinical symptoms, including cognitive symptoms, neuropsychiatric symptoms, and ADL, were evaluated using a series of rating scales. The relationships between RT and cognitive symptoms scores were analyzed in patients with AD. Reduced RT was found in 54.4% of patients with AD. The average RT, RT of the superior 1/2 quadrant, and RT of the inferior 1/2 quadrant of both eyes were all significantly decreased in the abnormal AD-RT group ( < 0.001). Overall cognitive function and performance in multiple cognitive domains, including memory, language, attention, and executive function, were also significantly impaired in the abnormal AD-RT group ( < 0.05). For lower RT value, the global cognitive function and the performance in multiple cognitive domains were worse. ADL was significantly compromised in patients with AD having lower RT values ( < 0.05). Lower RT value appear to be correlated with cognitive impairment, and RT may be an indicator of cognitive decline in patients with AD. Further studies are required to confirm our findings.
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http://dx.doi.org/10.3389/fnagi.2020.584244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878673PMC
January 2021

Theoretical and Experimental Study on Nonlinear Failure of an MEMS Accelerometer under Dual Frequency Acoustic Interference.

Sensors (Basel) 2021 Jan 31;21(3). Epub 2021 Jan 31.

Beijing Institute of Aerospace Control Device, Beijing 100094, China.

In order to quantitatively study the interfered output of the accelerometer under an acoustic injection attack, a mathematical model for fitting and predicting the accelerometer output was proposed. With ADXL103 as an example, an acoustic injection attack experiment with amplitude sweeping and frequency sweeping was performed. In the mathematical model, the R-squared coefficient was = 0.9990 in the acoustic injection attack experiment with amplitude sweeping, and = 0.9888 with frequency sweeping. Based on the mathematical model, the dual frequency acoustic injection attack mode was proposed. The difference frequency signal caused by the nonlinear effect was not filtered by the low-pass filter. At a 115 dB sound pressure level, the maximum acceleration bias of the output was 4.4 m/s and the maximum amplitude of fluctuation was 4.97 m/s. Two kinds of methods of prevention against acoustic injection attack were proposed, including changing the damping ratio of the accelerometer and adding a preposition low-pass filter.
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http://dx.doi.org/10.3390/s21030945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867008PMC
January 2021

Injectable Desferrioxamine-Laden Silk Nanofiber Hydrogels for Accelerating Diabetic Wound Healing.

ACS Biomater Sci Eng 2021 03 1;7(3):1147-1158. Epub 2021 Feb 1.

Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States.

Dysangiogenesis and chronic inflammation are two critical reasons for diabetic foot ulcers. Desferrioxamine (DFO) was used clinically in the treatment of diabetic foot ulcers by repeated injections because of its capacity to induce vascularization. Biocompatible carriers that release DFO slowly and facilitate healing simultaneously are preferable options to accelerate the healing of diabetic wounds. Here, DFO-laden silk nanofiber hydrogels that provided a sustained release of DFO for more than 40 days were used to treat diabetic wounds. The DFO-laden hydrogels stimulated the healing of diabetic wounds. In vitro cell studies revealed that the DFO-laden hydrogels modulated the migration and gene expression of endothelial cells, and they also tuned the inflammation behavior of macrophages. These results were confirmed in an in vivo diabetic wound model. The DFO-laden hydrogels alleviated dysangiogenesis and chronic inflammation in the diabetic wounds, resulting in a more rapid wound healing and increased collagen deposition. Both in vitro and in vivo studies suggested potential clinical applications of these DFO-laden hydrogels in the treatment of diabetic ulcers.
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http://dx.doi.org/10.1021/acsbiomaterials.0c01502DOI Listing
March 2021

Independent syntactic representation identified in left front-temporal cortex during Chinese sentence comprehension.

Brain Lang 2021 Mar 24;214:104907. Epub 2021 Jan 24.

School of Linguistics Sciences and Arts, Jiangsu Normal University, Xuzhou, China; Jiangsu Key Laboratory of Language and Cognitive Neuroscience, Xuzhou, China; Collaborative Innovation Center for Language Ability, Jiangsu Normal University, Xuzhou, China. Electronic address:

It has been well established that syntactic representation is independent of semantic representation in Indo-European languages, but it is unclear whether this is the case in Chinese. The present functional magnetic resonance imaging (fMRI) study adopted a syntactic priming paradigm to investigate the neural basis of Chinese syntactic representation. A passive sentence was preceded by either a passive or an active sentence without repeating a verb or a pattern of agent-patient animacy, thus constructing primed and unprimed sentence pairs based on sentence structure. The fMRI data were collected from 22 native Chinese speakers while they were reading the sentences. Priming-related activation suppression was found in the left temporal pole, left inferior frontal gyrus and left precentral gyrus. The results are the strongest neuroimaging evidence to date that syntactic representation is independent of semantic representation in Chinese, in line with Indo-European languages.
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http://dx.doi.org/10.1016/j.bandl.2021.104907DOI Listing
March 2021

MYBPC2 and MYL1 as Significant Gene Markers for Rhabdomyosarcoma.

Technol Cancer Res Treat 2021 Jan-Dec;20:1533033820979669

Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

Background: Rhabdomyosarcoma is the most common soft tissue tumor in children. Rhabdomyosarcoma commonly results in pain and bleeding caused by tumor compression and is prone to early metastasis and recurrence, which can seriously affect the therapeutic outcomes and long-term prognosis. Up to 37.7% of rhabdomyosarcomas may metastasize. Therefore, the molecular mechanisms underlying rhabdomyosarcoma must be explored to identify an effective target for its early diagnosis and specific treatment.

Methods: A dataset of 18 rhabdomyosarcoma tissue samples and 6 healthy skeletal muscle samples was downloaded. Differentially expressed genes between rhabdomyosarcoma and healthy tissue samples were identified by GEO2R. Kyoto Encyclopedia of Genes and Genomes and gene ontology pathway enrichment analyses were performed. A protein-protein interaction network was constructed, and hub genes were identified. Expression and survival analyses of hub genes were performed. Additionally, 30 patients with rhabdomyosarcoma were recruited, and overall survival information and samples were collected. Reverse transcription quantitative real-time polymerase chain reaction assays were performed to verify the expression of and in rhabdomyosarcoma tumor tissues. The Kaplan-Meier method was used to explore overall survival based on our clinical data.

Results: In total, 164 genes were up-regulated and 394 were down-regulated in rhabdomyosarcoma tumor tissues. Gene ontology analysis revealed that variations were predominantly enriched in the cell cycle, muscle contraction, muscle system processes, cytoskeleton, nucleotide binding, and cytoskeletal protein binding. The protein-protein interaction network revealed 3274 edges, and 441 nodes were constructed. Ten hub genes were identified; of these, and were significantly up-regulated in rhabdomyosarcoma. Compared with the healthy group, patients with rhabdomyosarcoma exhibiting high expression of and exhibited significantly worse overall survival.

Conclusions: We found differentially expressed genes between rhabdomyosarcoma and healthy tissue samples. and may be involved in the pathogenesis of rhabdomyosarcoma and therefore deserve further exploration.
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http://dx.doi.org/10.1177/1533033820979669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844451PMC
January 2021

Multiscale Assembly of [AgS ] Tetrahedrons into Hierarchical Ag-S Networks for Robust Photonic Water.

Adv Mater 2021 Feb 21;33(8):e2006459. Epub 2021 Jan 21.

Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117585, Singapore.

There is an urgent need to assemble ultrasmall metal chalcogenides (with atomic precision) into functional materials with the required anisotropy and uniformity, on a micro- or even macroscale. Here, a delicate yet simple chemistry is developed to produce a silver-sulfur network microplate with a high monodispersity in size and morphology. Spanning from the atomic, molecular, to nanometer, to micrometer scale, the key structural evolution of the obtained microplates includes 2D confinement growth, edge-sharing growth mode, and thermodynamically driven layer-by-layer stacking, all of which are derived from the [AgS ] tetrahedron unit. The key to such a high hierarchical, complex, and accurate assembly is the dense deprotonated ligand layer on the surface of the microplates, forming an infinite surface with high negative charge density. This feature operates at an orderly distance to allow further hierarchical self-assembly on the microscale to generate columnar assemblies composed of microplate components, thereby endowing the feature of the 1D photonic reflector to water (i.e., photonic water). The reflective color of the resulting photonic water is highly dependent on the thickness of the building blocks (i.e., silver-sulfur microplates), and the coexistent order and fluidity help to form robust photonic water.
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http://dx.doi.org/10.1002/adma.202006459DOI Listing
February 2021

Rationally Designed Antibody Drug Conjugates Targeting the Breast Cancer-Associated Endothelium.

ACS Biomater Sci Eng 2020 05 1;6(5):2563-2569. Epub 2019 Oct 1.

Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, Massachusetts 02115, United States.

The promise of antiangiogenic therapy for the treatment of breast cancer has been limited by the inability to selectively disrupt the established tumor vasculature. Here, we report the development of rationally designed antibody drug conjugates (ADCs) that can selectively recognize and attack breast tumor-associated endothelial cells (BTECs), while sparing normal endothelial cells (NECs). We first performed a quantitative and unbiased screening of a panel of cancer-related antigens on human BTECs and identified CD105 as the optimal ADC target on these cells. We then used clinically approved ADC linkers and cytotoxic drugs to engineer two CD105-targeted ADCs: CD105-DM1 and CD105-MMAE and evaluated their in vitro efficacy in human BTECs and NECs. We found that both CD105-DM1 and CD105-MMAE exhibited highly potent and selective cytotoxicity against BTECs with IC values of 3.2 and 3.7 nM, respectively, significantly lower than their IC values on NECs (8-13 fold). Our proof-of-principle study suggests that CD105-targeted ADCs are promising antiangiogenic agents that have the potential to be used to inhibit the established tumor vasculature of breast tumors in a safe and precise manner.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01060DOI Listing
May 2020

The complete chloroplast genome sequence of (Ulmaceae).

Mitochondrial DNA B Resour 2020 Jul 24;5(3):2957-2958. Epub 2020 Jul 24.

College of Life Sciences, Henan Agricultural University, Zhengzhou, China.

Jacq is a kind of landscape tree endemic to East Asia. In this study, the complete chloroplast genome of was sequenced. The genome was 159,259 bp in length, with a large single-copy (LSC) region of 88,451 bp, a small single-copy (SSC) region of 19,598 bp, and two inverted repeat (IR) regions of 25,605 bp, each. The genome consisted of 121 genes, including 77 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. The phylogenetic results indicated that is not monophyletic and constitute a well-supported clade sister to Sect. . The complete chloroplast genome of will provide important information for phylogenetic and evolutionary studies in Ulmaceae, as well as the other closely related families.
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http://dx.doi.org/10.1080/23802359.2020.1791006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782882PMC
July 2020

Discovery of novel 1,3,5-triazine derivatives as potent inhibitor of cervical cancer via dual inhibition of PI3K/mTOR.

Bioorg Med Chem 2021 Feb 6;32:115997. Epub 2021 Jan 6.

Department of Thyroid Breast Surgery, Maternal and Child Health Hospital of Hubei Province, No.745 Wuluo Road, Wuhan city, Hubei province 430070, China. Electronic address:

This study describes the synthesis of novel 1,3,5-triazine derivatives as potent inhibitors of cervical cancer. The compounds were initially tested for inhibition of PI3K/mTOR, where they showed significant inhibitory activity. The top-ranking molecule (compound 6 h) was further tested against class I PI3K isoforms, such as PI3Kα, PI3Kβ, PI3Kγ and PI3Kδ, where it showed the most significant activity against PI3Kα. Compound 6 h was then tested for anti-cancer activity against triple-negative breast cancer cells (MDA-MB321), human breast cancer cells (MCF-7), human cervical cancer cells (HeLa) and human liver cancer cells (HepG2), and it showed the greatest potency against HeLa cells. The effects of compound 6 h were further evaluated against the HeLa cells, where it showed significant attenuation of cell viability by inducing cell cycle arrest in the G1 phase. Compound 6 h induced apoptosis and reduced migration and invasion of HeLa cells. Western blotting analysis showed that 6 h inhibited PI3K and mTOR with positive modulation of Bcl-2 and Bax levels in HeLa cells. The effects of compound 6 h were also investigated in a tumour xenograft mouse model, where it showed reduction of tumour volume and weight. It also inhibited the PI3K/Akt/mTOR signalling cascade in xenograft tumour tissues, as evidenced by western blotting analysis. The results of the present study suggest the possible utility of the designed 1,3,5-triazine derivative as a potent inhibitor of cervical cancer.
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http://dx.doi.org/10.1016/j.bmc.2021.115997DOI Listing
February 2021

Histone demethylase JMJD2D promotes the self-renewal of liver cancer stem-like cells by enhancing EpCAM and Sox9 expression.

J Biol Chem 2020 11 24:100121. Epub 2020 Nov 24.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, China;. Electronic address:

Cancer stem-like cells (CSCs) contribute to the high rate of tumor heterogeneity, metastasis, therapeutic resistance, and recurrence. Histone lysine demethylase 4D (KDM4D or JMJD2D) is highly expressed in colon and liver tumors, where it promotes cancer progression; however, the role of JMJD2D in CSCs remains unclear. Here, we show that JMJD2D expression was increased in liver cancer stem-like cells (LCSCs); downregulation of JMJD2D inhibited the self-renewal of LCSCs in vitro and in vivo and inhibited the lung metastasis of LCSCs by reducing the survival and the early lung seeding of circulating LCSCs. Mechanistically, JMJD2D promoted LCSC self-renewal by enhancing the expression of CSC markers EpCAM and Sox9; JMJD2D reduced H3K9me3 levels on the promoters of EpCAM and Sox9 to enhance their transcription via interaction with β-catenin/TCF4 and Notch1 intracellular domain, respectively. Restoration of EpCAM and Sox9 expression in JMJD2D-knockdown liver cancer cells rescued the self-renewal of LCSCs. Pharmacological inhibition of JMJD2D using 5-c-8HQ reduced the self-renewal of LCSCs and liver cancer progression. Collectively, our findings suggest that JMJD2D promotes LCSC self-renewal by enhancing EpCAM and Sox9 expression via Wnt/β-catenin and Notch signaling pathways and is a potential therapeutic target for liver cancer.
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http://dx.doi.org/10.1074/jbc.RA120.015335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948496PMC
November 2020

The complete chloroplast genome sequence of (Bignoniaceae): a high-quality timber species from China.

Mitochondrial DNA B Resour 2020 Dec 24;5(4):3854-3855. Epub 2020 Dec 24.

College of Life Science, Henan Agricultural University, Zhengzhou, China.

is an important resource of timber, belonging to the genus (Bignoniaceae). In this study, we sequenced complete chloroplast (cp) genome of , using a NovaSeq 6000 sequencing platform. The genome of the was 152,153 bp in length, including a large single-copy region (84,910 bp), a small single-copy region (12,664 bp), and two inverted repeats regions (30,285 bp). It encodes 126 genes, including 81 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analyses were performed based on 15 cp genomes using the maximum likelihood (ML) method, supported that was probably more closely related to . . This study, the cp genome of was assembled, which will provide more theoretical basis for determine the phylogenetic relationships of the and related species.
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http://dx.doi.org/10.1080/23802359.2020.1841581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759281PMC
December 2020

Surgical treatment for antiplatelet intracerebral hemorrhage (SAP-ICH): protocol for a prospective cohort study of emergency surgery for severe spontaneous intracerebral hemorrhage patients on long-term oral antiplatelet treatment.

Chin Neurosurg J 2021 Jan 11;7(1). Epub 2021 Jan 11.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No.119 South 4th Ring West Road, Fengtai District, Beijing, 100070, China.

Background: Despite the capability of emergency surgery to reduce the mortality of severe spontaneous intracranial hemorrhage (SSICH) patients, the effect and safety of surgical treatment for severe spontaneous intracranial hemorrhage (SSICH) patients receiving long-term oral antiplatelet treatment (LOAPT) remains unclear. In consideration of this, the cohort study is aimed at figuring out the effect and safety of emergency surgery for SSICH patients on LOAPT.

Methods: As a multicenter and prospective cohort study, it will be conducted across 7 representative clinical centers. Starting in September 2019, the observation is scheduled to be completed by December 2022, with a total of 450 SSICH patients recruited. The information on clinical, radiological, and laboratory practices will be recorded objectively. All of the patients will be monitored until death or 6 months after the occurrence of primary hemorrhage.

Results: In this study, two comparative cohorts and an observational cohort will be set up. The primary outcome is the effect of emergency surgery, which is subject to assessment using the total mortality and comparison in the survival rate of SSICH patients on LOAPT between surgical treatment and conservative treatment. The second outcome is the safety of surgery, with the postoperative hemorrhagic complication which is compared between the operated SSICH patients on and not on LOAPT. Based on the observation of the characteristics and outcome of SSICH patients on LOAPT, the ischemic events after discontinuing LOAPT will be further addressed, and the coagulation function assessment system for operated SSICH patients on LOAPT will be established.

Conclusions: In this study, we will investigate the effect and safety of emergency surgery for SSICH patients on LOAPT, which will provide an evidence for management in the future.

Ethics And Dissemination: The research protocol and informed consent in this study were approved by the Institutional Review Board of Beijing Tiantan Hospital (KY2019-096-02). The results of this study are expected to be disseminated in peer-reviewed journals in 2023.

Trial Registration: Name: Effect and safety of surgical intervention for severe spontaneous intracerebral hemorrhage patients on long-term oral antiplatelet treatment. ChiCTR1900024406 . Date of registration is July 10, 2019.
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http://dx.doi.org/10.1186/s41016-020-00225-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798270PMC
January 2021