Publications by authors named "Peng Gao"

1,592 Publications

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Covalent organic framework-engineered polydopamine nanoplatform for multimodal imaging-guided tumor photothermal-chemotherapy.

Chem Commun (Camb) 2021 May 11. Epub 2021 May 11.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

A covalent organic framework (COF)-engineered polydopamine core-shell nanoplatform (PDA@COF) was developed. The ultrasmall pores and abundant functional sites of the COF endowed the nanoplatform with enhanced drug loading capacity and diminished drug leakage effect. Multimodal imaging-guided photothermal chemo-synergistic tumor-targeted therapy was realized after rational functionalization. This work offers new insights for developing COF-based multifunctional theranostic systems.
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http://dx.doi.org/10.1039/d1cc00314cDOI Listing
May 2021

Characteristics of inflammatory phenotypes among patients with asthma: relationships of blood count parameters with sputum cellular phenotypes.

Allergy Asthma Clin Immunol 2021 May 11;17(1):47. Epub 2021 May 11.

Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun, 130041, Jilin, China.

Background: There is a need to identify the asthma inflammatory phenotypes of patients to facilitate personalized asthma treatment. Sputum induction is time-consuming and requires expert clinical technique. This study aimed to assess the distribution and characteristics of asthma inflammatory phenotypes in Jilin Province, China; it also aimed to identify an easier method for characterization of an asthma phenotype, rather than sputum cellular analysis.

Methods: In this study, 232 asthma patients underwent sputum induction following clinical assessment and blood collection. Inflammatory cell counts in sputum were used to classify asthma inflammatory phenotypes. Receiver operating characteristic curve and Spearman correlation coefficient analyses were used to identify correlations between clinical parameters.

Results: Among the included patients, there had 52.1% paucigranulocytic, 38.4% eosinophilic, 4.3% neutrophilic, and 5.2% mixed granulocytic asthma phenotypes, respectively. In total, 129 (55.6%) patients had asthma-chronic obstructive pulmonary disease (COPD) overlap (ACO); these patients had higher proportion of smokers, higher sputum neutrophil count, worse lung function, and worse asthma control, compared with patients who had asthma alone (p < 0.05). Sputum eosinophil/neutrophil counts were positively correlated with blood eosinophil/neutrophil counts (p < 0.01). To identify the presence of sputum eosinophil proportion ≥ 3%, optimal cut-off values for blood eosinophil count and fractional exhaled nitric oxide (FeNO) were 0.2 × 10/L and 30.25 ppd (area under the curve (AUC) = 0.744; AUC = 0.653, p < 0.001). AUCs did not significantly differ between FeNO and blood eosinophil count (p = 0.162), but both exhibited poor specificity (57% and 49%, respectively). To identify the presence of sputum neutrophil proportion ≥ 61%, the optimal cut-off value for blood neutrophil proportion was 69.3% (AUC = 0.691, p = 0.0003); however, this exhibited poor sensitivity (50%).

Conclusions: Paucigranulocytic asthma was the most common phenotype, followed by eosinophilic asthma. Higher proportion of smokers, poor patient compliance, insufficient treatment, and poor asthma control may have been the main causes of high ACO proportion among patients in this study. Blood eosinophil/neutrophil counts exhibited poor specificity and sensitivity for prediction of airway eosinophilic/neutrophilic inflammation.
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http://dx.doi.org/10.1186/s13223-021-00548-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111745PMC
May 2021

Water-Triggered Transformation of Ligand-Free Lead Halide Perovskite Nanocrystal-Embedded Pb(OH)Br with Ultrahigh Stability.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, No. 122, Luoshi Road, Wuhan 430070, P. R. China.

Lead halide perovskite (LHP) nanomaterials have attracted tremendous attention owing to their remarkable optoelectronic properties. However, they are extremely unstable under moist environments, high temperatures, and light illumination due to their intrinsic structural lability, which has been the critical unsolved problem for practical applications. To address this issue, we propose a facile and environmentally friendly ligand-free approach to design and synthesize rod-like CsPbBr-embedded Pb(OH)Br with excellent stability under various harsh environments such as soaking in water, heating, and ultraviolet (UV) illumination. Plate-like CsPbBr- and CsPbBr-embedded Pb(OH)Br powders are first formed by evaporating the solvent in a dispersion of ethanol (or methanol, isopropanol), CsCO, and PbBr. Upon soaking in water, the plate-like sample undergoes phase transformation from CsPbBr and CsPbBr to CsPbBr and shape conversion from nanoplate to a microrod, leading to the formation of rod-like CsPbBr-embedded Pb(OH)Br. The stable Pb(OH)Br coating effectively prevents the luminescent CsPbBr nanocrystals from reacting with water, leading to extremely high aqueous stability of the CsPbBr-embedded Pb(OH)Br. The photoluminescence (PL) intensity of the representative CsPbBr-embedded Pb(OH)Br sample can maintain 92.2% of the initial PL intensity value even after soaking in room-temperature water for 165 days; in the meantime, the phase and shape are preserved. The typical sample also shows outstanding stability under hot water, UV illumination, and annealing conditions. The ultrahigh aqueous stability, thermal stability, and photostability of the CsPbBr-embedded Pb(OH)Br nanomaterials suggest an effective, facile, and environmentally friendly technique to grow perovskite-based nanomaterials for promising practical applications in the optoelectronic field.
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http://dx.doi.org/10.1021/acsami.1c06627DOI Listing
May 2021

Prototype Design of a Domain-Wall-Based Magnetic Memory Using a Single Layer LaSrMnO Thin Film.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

Department of Physics, Beijing Normal University, Beijing, China.

Magnetic field-free, nonvolatile magnetic memory with low power consumption is highly desired in information technology. In this work, we report a current-controllable alignment of magnetic domain walls in a single layer LaSrMnO thin film with the threshold current density of 2 × 10 A/cm at room temperature. The vector relationship between current directions and domain-wall orientations indicates the dominant role of spin-orbit torque without an assistance of external magnetic field. Meanwhile, significant planar Hall resistances can be readout in a nonvolatile way before and after the domain-wall reorientation. A domain-wall-based magnetic random-access memory (DW-MRAM) prototype device has been proposed.
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http://dx.doi.org/10.1021/acsami.1c04724DOI Listing
May 2021

The prognostic significance of electrocardiography findings in patients with coronavirus disease 2019: A retrospective study.

Clin Cardiol 2021 May 11. Epub 2021 May 11.

Department of Cardiology, Intervention Cardiology Center, Wuhan No.1 Hospital, No.215 Zhongshan Avenue, QiaoKou District, Wuhan, China.

Background: Coronavirus disease 2019 (COVID-19) has reached a pandemic level. Cardiac injury is not uncommon among COVID-19 patients. We sought to describe the electrocardiographic characteristics and to identify the prognostic significance of electrocardiography (ECG) findings of patients with COVID-19.

Hypothesis: ECG abnormality was associated with higher risk of death.

Methods: Consecutive patients with laboratory-confirmed COVID-19 and definite in-hospital outcome were retrospectively included. Demographic characteristics and clinical data were extracted from medical record. Initial ECGs at admission or during hospitalization were reviewed. A point-based scoring system of abnormal ECG findings was formed, in which 1 point each was assigned for the presence of axis deviation, arrhythmias, atrioventricular block, conduction tissue disease, QTc interval prolongation, pathological Q wave, ST-segment change, and T-wave change. The association between abnormal ECG scores and in-hospital mortality was assessed in multivariable Cox regression models.

Results: A total of 306 patients (mean 62.84 ± 14.69 years old, 48.0% male) were included. T-wave change (31.7%), QTc interval prolongation (30.1%), and arrhythmias (16.3%) were three most common found ECG abnormalities. 30 (9.80%) patients died during hospitalization. Abnormal ECG scores were significantly higher among non-survivors (median 2 points vs 1 point, p < 0.001). The risk of in-hospital death increased by a factor of 1.478 (HR 1.478, 95% CI 1.131-1.933, p = 0.004) after adjusted by age, comorbidities, cardiac injury and treatments.

Conclusions: ECG abnormality was common in patients admitted for COVID-19 and was associated with adverse in-hospital outcome. In-hospital mortality risk increased with increasing abnormal ECG scores.
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http://dx.doi.org/10.1002/clc.23628DOI Listing
May 2021

Two-Dimensional BiSrCaCuO Nanosheets for Ultrafast Photonics and Optoelectronics.

ACS Nano 2021 May 10. Epub 2021 May 10.

Department of Materials Science and Engineering, and ARC Centre of Excellence in Future Low-Energy Electronics Technologies, Monash University, Clayton, Victoria 3800, Australia.

Two-dimensional (2D) BiSrCaCuO (BSCCO) is a emerming class of 2D materials with high-temperature superconductivity for which their electronic transport properties have been intensively studied. However, the optical properties, especially nonlinear optical response and the photonic and optoelectronic applications of normal state 2D BiSrCaCuO (Bi-2212), have been largely unexplored. Here, the linear and nonlinear optical properties of mechanically exfoliated Bi-2212 thin flakes are systematically investigated. 2D Bi-2212 shows a profound plasmon absorption in near-infrared wavelength range with ultrafast carrier dynamics as well as tunable nonlinear absorption depending on the thickness. We demonstrated that 2D Bi-2212 can be applied not only as an effective mode-locker for ultrashort pulse generation but also as an active medium for infrared light detection due to its plasmon absorption. Our results may trigger follow up studies on the optical properties of 2D BSCCO and demonstrate potential opportunities for photonic and optoelectronic applications.
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http://dx.doi.org/10.1021/acsnano.1c01567DOI Listing
May 2021

Conserved, divergent and heterochronic gene expression during Brachypodium and Arabidopsis embryo development.

Plant Reprod 2021 May 5. Epub 2021 May 5.

Laboratory of Biochemistry, Wageningen University, Stippeneng 4, Wageningen, The Netherlands.

Key Message: Developmental and transcriptomic analysis of Brachypodium embryogenesis and comparison with Arabidopsis identifies conserved and divergent phases of embryogenesis and reveals widespread heterochrony of developmental gene expression. Embryogenesis, transforming the zygote into the mature embryo, represents a fundamental process for all flowering plants. Current knowledge of cell specification and differentiation during plant embryogenesis is largely based on studies of the dicot model plant Arabidopsis thaliana. However, the major crops are monocots and the transcriptional programs associated with the differentiation processes during embryogenesis in this clade were largely unknown. Here, we combined analysis of cell division patterns with development of a temporal transcriptomic resource during embryogenesis of the monocot model plant Brachypodium distachyon. We found that early divisions of the Brachypodium embryo were highly regular, while later stages were marked by less stereotypic patterns. Comparative transcriptomic analysis between Brachypodium and Arabidopsis revealed that early and late embryogenesis shared a common transcriptional program, whereas mid-embryogenesis was divergent between species. Analysis of orthology groups revealed widespread heterochronic expression of potential developmental regulators between the species. Interestingly, Brachypodium genes tend to be expressed at earlier stages than Arabidopsis counterparts, which suggests that embryo patterning may occur early during Brachypodium embryogenesis. Detailed investigation of auxin-related genes shows that the capacity to synthesize, transport and respond to auxin is established early in the embryo. However, while early PIN1 polarity could be confirmed, it is unclear if an active response is mounted. This study presents a resource for studying Brachypodium and grass embryogenesis and shows that divergent angiosperms share a conserved genetic program that is marked by heterochronic gene expression.
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http://dx.doi.org/10.1007/s00497-021-00413-4DOI Listing
May 2021

Effect of Cu Content on Performance of Sn-Zn-Cu Lead-Free Solder Alloys Designed by Cluster-Plus-Glue-Atom Model.

Materials (Basel) 2021 Apr 30;14(9). Epub 2021 Apr 30.

Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, China.

The mechanical properties of solder alloys are a performance that cannot be ignored in the field of electronic packaging. In the present study, novel Sn-Zn solder alloys were designed by the cluster-plus-glue-atom (CPGA) model. The effect of copper (Cu) addition on the microstructure, tensile properties, wettability, interfacial characterization and melting behavior of the Sn-Zn-Cu solder alloys were investigated. The SnZnCu solder alloy exhibited a fine microstructure, but the excessive substitution of the Cu atoms in the CPGA model resulted in extremely coarse intermetallic compound (IMC). The tensile tests revealed that with the increase in Cu content, the tensile strength of the solder alloy first increased and then slightly decreased, while its elongation increased slightly first and then decreased slightly. The tensile strength of the SnZnCu solder alloy reached 95.3 MPa, which was 57% higher than the plain Sn-Zn solder alloy, which is attributed to the fine microstructure and second phase strengthening. The spreadability property analysis indicated that the wettability of the Sn-Zn-Cu solder alloys firstly increased and then decreased with the increase in Cu content. The spreading area of the SnZnCu solder alloy was increased by 27.8% compared to that of the plain Sn-Zn solder due to Cu consuming excessive free state Zn. With the increase in Cu content, the thickness of the IMC layer decreased owing to Cu diminishing the diffusion force of Zn element to the interface.
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http://dx.doi.org/10.3390/ma14092335DOI Listing
April 2021

Efficient and selective adsorption of methylene blue and methyl violet dyes by yellow passion fruit peel.

Environ Technol 2021 May 4:1-16. Epub 2021 May 4.

Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology for Science and Education Combined with Science and Technology Innovation Base, Guilin University of Technology, Guilin, Guangxi 541004, China.

As an important biomass resource, agricultural waste is of great significance to improve the application value. In this study, the yellow passion fruit peel (Y-PFP) was used as a biosorbent to remove cationic dyes (methylene blue (MB) and methyl violet (MV)) by the simple treatment process. And the effects of pH, contact time, initial dye concentration, ionic strength, and temperature on the adsorption performance of Y-PFP were studied. The adsorption process was consistent with the pseudo-second-order kinetic model and Langmuir isotherm. What's more, the maximum adsorption capacity for MB and MV was 324.7 mg·g and 485.4 mg·g, respectively. And Y-PFP still exhibited high removal efficiency after five desorption-adsorption cycles. Thus, Y-PFP had highly valuable for the removal of cationic dyes from wastewater with simple preparation process, low cost, excellent adsorption capacity and selectivity.
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http://dx.doi.org/10.1080/09593330.2021.1924288DOI Listing
May 2021

An effective self-supervised framework for learning expressive molecular global representations to drug discovery.

Brief Bioinform 2021 May 3. Epub 2021 May 3.

Tsinghua Laboratory of Brain and Intelligence and Department of Biomedical Engineering, Tsinghua University, Haidian, 100084 Beijing, China.

How to produce expressive molecular representations is a fundamental challenge in artificial intelligence-driven drug discovery. Graph neural network (GNN) has emerged as a powerful technique for modeling molecular data. However, previous supervised approaches usually suffer from the scarcity of labeled data and poor generalization capability. Here, we propose a novel molecular pre-training graph-based deep learning framework, named MPG, that learns molecular representations from large-scale unlabeled molecules. In MPG, we proposed a powerful GNN for modelling molecular graph named MolGNet, and designed an effective self-supervised strategy for pre-training the model at both the node and graph-level. After pre-training on 11 million unlabeled molecules, we revealed that MolGNet can capture valuable chemical insights to produce interpretable representation. The pre-trained MolGNet can be fine-tuned with just one additional output layer to create state-of-the-art models for a wide range of drug discovery tasks, including molecular properties prediction, drug-drug interaction and drug-target interaction, on 14 benchmark datasets. The pre-trained MolGNet in MPG has the potential to become an advanced molecular encoder in the drug discovery pipeline.
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http://dx.doi.org/10.1093/bib/bbab109DOI Listing
May 2021

Pharmacokinetic comparisons of six steroid saponins in rat plasma following oral administration of crude and stir-fried Fructus Tribuli extracts by UHPLC-MS/MS.

Biomed Chromatogr 2021 May 3:e5151. Epub 2021 May 3.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Modern pharmacological studies have shown that Fructus Tribuli can improve sexual function and treat cardiovascular diseases. In this study, we focused on comparing the pharmacokinetics of crude Fructus Tribuli (CFT) and stir-fried Fructus Tribuli (SFT) to further clarify the changes in chemical composition in vivo. The quantitation of six analytes was performed in a triple quadrupole mass spectrometer using the multiple reaction monitoring mode. Separation was performed on Halo® C18 column using 0.05% formic acid and 5 μmoL/L sodium formate in water, and 0.05% formic acid and 5 μmoL/L sodium formate in acetonitrile as the mobile phase. The selectivity, precision, accuracy, extraction recovery, matrix effect, and stability of the method were fully validated. Compared to the crude group, the parameters of C and AUC of terrestroside B and terrestrosin K increased significantly (p < 0.05), but C and AUC of polianthoside D, terrestrinin D, tribuluside A, and terrestrosin D were decreased, terrestrosin D was especially decreased (p < 0.05), after oral administration of SFT extract. These results showed that the developed method was suitable for pharmacokinetic analysis of the six steroid saponins of CFT and SFT in rat plasma, and can be used to facilitate future clinical studies.
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http://dx.doi.org/10.1002/bmc.5151DOI Listing
May 2021

Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance.

Sci Adv 2021 Apr 30;7(18). Epub 2021 Apr 30.

Center for Vascular and Developmental Biology, Feinberg Cardiovascular and Renal Research Institute, Northwestern University, Chicago, IL, USA.

Recent findings indicate that mitochondrial respiration regulates blood endothelial cell proliferation; however, its role in differentiating lymphatic endothelial cells (LECs) is unknown. We hypothesized that mitochondria could work as a sensor of LECs' metabolic specific needs by determining their functional requirements according to their differentiation status and local tissue microenvironment. Accordingly, we conditionally deleted the QPC subunit of mitochondrial complex III in differentiating LECs of mouse embryos. Unexpectedly, mutant mice were devoid of a lymphatic vasculature by mid-gestation, a consequence of the specific down-regulation of main LEC fate regulators, particularly Vegfr3, leading to the loss of LEC fate. Mechanistically, this is a result of reduced H3K4me3 and H3K27ac in the genomic locus of key LEC fate controllers (e.g., and ). Our findings indicate that by sensing the LEC differentiation status and microenvironmental metabolic conditions, mitochondrial complex III regulates the critical Prox1-Vegfr3 feedback loop and, therefore, LEC fate specification and maintenance.
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http://dx.doi.org/10.1126/sciadv.abe7359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087398PMC
April 2021

Axial line-scanning stimulated emission depletion fluorescence correlation spectroscopy.

Opt Lett 2021 May;46(9):2184-2187

Investigating the dynamics and interactions of biomolecules within or attached to membranes of living cells is crucial for understanding biology at the molecular level. In this pursuit, classical, diffraction-limited optical fluorescence microscopy is widely used, but it faces limitations due to (1) the heterogeneity of biomembranes on the nanoscale and (2) the intrinsic motion of membranes with respect to the focus. Here we introduce a new confocal microscopy-based fluctuation spectroscopy technique aimed at alleviating these two problems, called axial line-scanning stimulated emission depletion fluorescence correlation spectroscopy (axial ls-STED-FCS). Axial line scanning by means of a tunable acoustic gradient index of refraction lens provides a time resolution of a few microseconds, which is more than two orders of magnitude greater than that of conventional, lateral line-scanning fluorescence correlation spectroscopy (typically around 1 ms). Using STED excitation, the observation area on the membrane can be reduced 10-100 fold, resulting in sub-diffraction spatial resolution and the ability to study samples with densely labeled membranes. Due to these attractive properties, we expect that the axial ls-STED-FCS will find wide application, especially in the biomolecular sciences.
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http://dx.doi.org/10.1364/OL.420765DOI Listing
May 2021

Green Synthesis and Incorporation of Sericin Silver Nanoclusters into Electrospun Ultrafine Cellulose Acetate Fibers for Anti-Bacterial Applications.

Polymers (Basel) 2021 Apr 27;13(9). Epub 2021 Apr 27.

Center of Excellence in Nanotechnology and Materials, Mehran University of Engineering and Technology, Jamshoro 76060, Pakistan.

Fiber based antibacterial materials have gained an enormous attraction for the researchers in these days. In this study, a novel Sericin Encapsulated Silver Nanoclusters (sericin-AgNCs) were synthesized through single pot and green synthesis route. Subsequently these sericin-AgNCs were incorporated into ultrafine electrospun cellulose acetate (CA) fibers for assessing the antibacterial performance. The physicochemical properties of sericin-AgNCs/CA composite fibers were investigated by transmission electron microscopy (TEM), field emission electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and wide X-ray diffraction (XRD). The antibacterial properties of sericin-AgNCs/CA composite fibers against () and () were systematically evaluated. The results showed that sericin-AgNCs incorporated in ultrafine CA fibers have played a vital role for antibacterial activity. An amount of 0.17 mg/mL sericin-AgNCs to CA fibers showed more than 90% results and elevated upto >99.9% with 1.7 mg/mL of sericin-AgNCs against . The study indicated that sericin-AgNCs/CA composite confirms an enhanced antibacterial efficiency, which could be used as a promising antibacterial product.
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http://dx.doi.org/10.3390/polym13091411DOI Listing
April 2021

Zone-Folded Longitudinal Acoustic Phonons Driving Self-Trapped State Emission in Colloidal CdSe Nanoplatelet Superlattices.

Nano Lett 2021 Apr 29. Epub 2021 Apr 29.

CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P.R. China.

Colloidal CdSe nanoplatelets (NPLs) have substantial potential in light-emitting applications because of their quantum-well-like characteristics. The self-trapped state (STS), originating from strong electron-phonon coupling (EPC), is promising in white light luminance because of its broadband emission. However, achieving STS in CdSe NPLs is extremely challenging because of their intrinsic weak EPC nature. Herein, we developed a strong STS emission in the spectral range of 450-600 nm by building superlattice (SL) structures with colloidal CdSe NPLs. We demonstrated that STS is generated via strong coupling of excitons and zone-folded longitudinal acoustic phonons with formation time of ∼450 fs and localization length of ∼0.56 nm. The Huang-Rhys factor, describing the EPC strength in SL structure, is estimated to be ∼19.9, which is much larger than that (∼0.1) of monodispersed CdSe NPLs. Our results provide an in-depth understanding of STS and a platform for generating and manipulating STS by designing SL structures.
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http://dx.doi.org/10.1021/acs.nanolett.0c04169DOI Listing
April 2021

1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling.

Front Cell Dev Biol 2021 12;9:657623. Epub 2021 Apr 12.

Department of Occupational Health, Third Military Medical University, Chongqing, China.

The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are not fully understood. Here, based on high-throughput RNA sequencing techniques, we revealed that transcripts related to neurite development were significantly influenced by 1800 MHz RF-EMF exposure during neuronal differentiation. Exposure to RF-EMF remarkably decreased the total length of neurite and the number of branch points in neural stem cells-derived neurons and retinoic acid-induced Neuro-2A cells. The expression of Eph receptors 5 (EPHA5), which is required for neurite outgrowth, was inhibited remarkably after RF-EMF exposure. Enhancing EPHA5 signaling rescued the inhibitory effects of RF-EMF on neurite outgrowth. Besides, we identified that cAMP-response element-binding protein (CREB) and RhoA were critical downstream factors of EPHA5 signaling in mediating the inhibitory effects of RF-EMF on neurite outgrowth. Together, our finding revealed that RF-EMF exposure impaired neurite outgrowth through EPHA5 signaling. This finding explored the effects and key mechanisms of how RF-EMF exposure impaired neurite outgrowth and also provided a new clue to understanding the influences of RF-EMF on brain development.
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http://dx.doi.org/10.3389/fcell.2021.657623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075058PMC
April 2021

Direct visualization of photo-induced disulfide through oxidative coupling of -aminothiophenol.

Chem Commun (Camb) 2021 Apr;57(34):4190-4193

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China.

Chemical transformations under visible irradiation are interesting in green preparation. Herein, a photo-oxidative coupling reaction of para-aminothiophenol (p-ATP) dimerizing to 4-aminophenyl disulfide (APDS) rather than 4,4'-dimercaptoazobenzene (DMAB) was achieved in water by visible light irradiation, producing monodispersed organic nanoparticles in situ with strong light scattering visualized by the dark-field microscopy (DFM) imaging technique, owing to the formation of disulfide.
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http://dx.doi.org/10.1039/d1cc00587aDOI Listing
April 2021

[Clinical application of vascularized free fibular bone flap for repairing segmental mandibular defects in 40 cases].

Shanghai Kou Qiang Yi Xue 2021 Feb;30(1):93-96

Department of Stomatology, Qinghai Red Cross Hospital. Xining 810000, Qinghai Province, China.

Purpose: To investigate the effect of vascularized free fibular flap in repairing segmental mandibular defects.

Methods: Eighty patients with segmental mandibular defects treated in our hospital from June 2015 to May 2018 were enrolled. Both groups of patients were operated by the same group of medical staff with more than 5 years of clinical experience. Patients were divided into 2 groups using a random number table method, 40 in each group. Patients in the control group underwent non-vascularized iliac bone graft while patients in the experimental, group underwent vascularized free fibular bone flap repair. The graft survival, pain, quality of life, negative emotions and complications were compared between the two groups using SPSS 22.0 software package.

Results: There was no significant difference in the scores of the proximal gingival papilla, distal gingival papilla, labial margin and labial margin (P>0.05). The gingival texture, color score and total score of the root surface of the experimental group were significantly higher than the control group (P<0.05). The scores of VAS, anxiety and depression in both groups were significantly decreased after operation, but the decrease in the experimental group was greater(P<0.05). Physical function, psychological and social function, life quality of the two groups were significantly increased, but the increase of the experimental group was greater (P<0.05).Three cases (7.5%) developed complications in the control group, including 1 case of pneumonia, 1 case of infection and 1 case of wound recurrence. Two cases(5%) in the experimental group developed of complications, including 1 case of wound recurrence and 1 case of pneumonia, the difference was not statistically significant (P>0.05).

Conclusions: Repair of mandibular segmental defect with vascularized free fibular bone flap can significantly improve the overall effect, relieve pain and negative emotion, improve quality of life of patients and decrease incidence of complications.
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February 2021

Three dimensional band-filling control of complex oxides triggered by interfacial electron transfer.

Nat Commun 2021 Apr 27;12(1):2447. Epub 2021 Apr 27.

Beijing National Laboratory for Condensed Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing, China.

The d-band-filling of transition metals in complex oxides plays an essential role in determining their structural, electronic and magnetic properties. Traditionally, at the oxide heterointerface, band-filling control has been achieved via electrostatic modification in the structure of field-effect transistors or electron transfer, which is limited to the quasi-two-dimension at the interface. Here we report a three-dimensional (3D) band-filling control by changing the local lattice coordination in a designed oxide heterostructure. At the LaCoO/LaTiO heterointerface, due to the Fermi level mismatch, electrons transfer from LaTiO to LaCoO. This triggers destabilisation of the CoO octahedrons, i.e. the formation of lattice configurations with a reduced Co valence. The associated oxygen migration results in the 3D topotactic phase transition of LaCoO. Tuned by the thickness of LaTiO, different crystalline phases and band-fillings of Co occur, leading to the emergence of different magnetic ground states.
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http://dx.doi.org/10.1038/s41467-021-22790-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079372PMC
April 2021

S100A11 Promotes Glioma Cell Proliferation and Predicts Grade-Correlated Unfavorable Prognosis.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211011961

Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical University, Hefei, China.

The prognosis of glioma is significantly correlated with the pathological grades; however, the correlations between the prognostic biomarkers with pathological grades have not been elucidated. S100A11 is involved in a variety of malignant biological processes of tumor, whereas its biological and clinicopathological features on glioma remain unclear. In this study, the S100A11 expression and clinical information were obtained from the public databases (TCGA, GEPIA2) to analyze its correlations with the pathological grade and the prognosis of glioma patients. We then verified the expression of S100A11 by immunohistochemistry staining. The effects of S100A11 on the proliferation of glioma cells were confirmed by cytological function assays (CCK-8, Flow cytometry, Clone formation assay) , the role of S100A11 in regulation of glioma growth was determined by xenograft model assay. We observed that S100A11 expression positively correlated with the pathological grades, while negatively correlated with the survival time of patients. In cytological analysis, we found the proliferations of glioma cell lines were significantly inhibited ( < 0.05) after interfering S100A11 expression via shRNAs. The cell cycle was blocked at G0/G1 stage. The ability of clone formation was significantly decreased, and the tumorigenicity was weakened ( < 0.05). In summary, S100A11 was over-expressed in gliomas and positively correlated with the pathological grades. Interfering the expression of S100A11 significantly inhibited the proliferation of glioma and the tumorigenicity ( < 0.05). In conclusion, S100A11 might be considered as a potential biomarker in glioma.
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http://dx.doi.org/10.1177/15330338211011961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085370PMC
April 2021

Rapid identification, isolation, and evaluation on anti-neuroinflammatory activity of limonoids derivatives from the root bark of Dictamnus dasycarpus.

J Pharm Biomed Anal 2021 Jun 18;200:114079. Epub 2021 Apr 18.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, People's Republic of China. Electronic address:

A total of 49 limonoids derivatives were rapidly identified by UNIFI software and three new limonoids derivatives, named dasycarinone (1, DAS), isodictamdiol C (2) and dasycarinone A (3), along with nineteen known compounds, were isolated from the root bark of Dictamnus dasycarpus, named as "Baixianpi" in Chinese. Their structures were elucidated on the basis of spectroscopic data (UV, IR, HR-ESI-MS, NMR, CD spectra and OR). All the compounds were tested for anti-inflammatory activities by suppressing the nitric oxide (NO) production in lipopolysaccharide (LPS) induced BV-2 cells. DAS exhibited a strong anti-inflammatory activity with IC value of 1.8 μM. Nuclear Factor kappa B (NF-κB) luciferase assay and enzyme-linked immune sorbent assay indicated that DAS can suppress the release of inflammatory cytokines such as Tumor Necrosis Factor α (TNF-α), interleukin 6 (IL-6) via inactivating NF-κB signaling pathways. Moreover, we found that anti-inflammatory activities of obacunone-class are better than those of limonin-class by analyzing structure-activity relationship. Our results suggested that obacunone derivatives play an important role on anti-inflammation of Baixianpi. As a representative among them, DAS showed a strong anti-inflammatory activity via suppressing NF-κB signaling pathways.
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http://dx.doi.org/10.1016/j.jpba.2021.114079DOI Listing
June 2021

Effect of additives on properties and microstructure of lightweight aggregates produced from MSWI bottom ash sludge (MSWI-BAS).

J Air Waste Manag Assoc 2021 Apr 26. Epub 2021 Apr 26.

School of Environment, Nanjing Normal University, Nanjing, China.

In order to solve problems of land occupation and environment damage resulted from massive municipal solid waste incineration bottom ash sludge (MSWI-BAS), sintered lightweight aggregates (LWA) was prepared from MSWI-BAS. Additives are of great significance for the preparation of high-performance LWA and the utilization of MSWI-BAS resources, so their effect on properties of LWA was investigated. The results showed that when the content of water glass was 20%, compressive strength of LWA reached a maximum of 8.4 MPa, and 1h water absorption reached a minimum of 5%. The reason was the addition of water glass brought a lot of Na and Si(OH), the internal crystals of water glass were converted into rod-shaped zeolite crystals, thereby forming a high-density structure. The addition of coal powder led to the formation of gas in LWA, thus reducing the density of LWA. At the same time, it was also conducive to earlier generation of liquid phase in LWA, making its internal structure dense. When the content of coal powder was 5 %, 15 % and 20 %, the modification effect was better, and compressive strength of LWA was larger, about 4MPa. Additives are of great significance for the preparation of high-performance LWA and the utilization of MSWI-BAS resources. Implication StatementIn this study we have prepared LWA with MSWI-BAS. At the same time of XRD and FT-IR analysis of raw materials, we also investigated effect of water glass and coal powder on characteristics (particle density, 1h water absorption and compressive strength) of lightweight aggregates, and good results were obtained. For explanations, several characterizations were carried out, such as XRD, SEM and so on. The sludge disposal problem is reduced. It opens up a new way for the utilization of solid waste resources. In addition, it meets with the concept of green development of building materials, and makes the production of LWA have a broader development prospect.
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http://dx.doi.org/10.1080/10962247.2021.1918288DOI Listing
April 2021

A Highly Strained Phase in PbZrTiO Films with Enhanced Ferroelectric Properties.

Adv Sci (Weinh) 2021 Apr 18;8(8):2003582. Epub 2021 Feb 18.

Department of Physics Southern University of Science and Technology Shenzhen Guangdong 518055 China.

Although epitaxial strain imparted by lattice mismatch between a film and the underlying substrate has led to distinct structures and emergent functionalities, the discrete lattice parameters of limited substrates, combined with strain relaxations driven by film thickness, result in severe obstructions to subtly regulate electro-elastic coupling properties in perovskite ferroelectric films. Here a practical and universal method to achieve highly strained phases with large tetragonal distortions in Pb-based ferroelectric films through synergetic effects of moderately (≈1.0%) misfit strains and laser fluences during pulsed laser deposition process is demonstrated. The phase possesses unexpectedly large Poisson's ratio and negative thermal expansion, and concomitant enhancements of spontaneous polarization (≈100 µC cm) and Curie temperature (≈800 °C), 40% and 75% larger than that of bulk counterparts, respectively. This strategy efficiently circumvents the long-standing issue of limited numbers of discrete substrates and enables continuous regulations of exploitable lattice states in functional oxide films with tightly elastic coupled performances beyond their present levels.
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http://dx.doi.org/10.1002/advs.202003582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061395PMC
April 2021

Integrative Modeling of Gene Expression and Metabolic Networks of Embryos for Identification of Seed Oil Causal Genes.

Front Plant Sci 2021 6;12:642938. Epub 2021 Apr 6.

Laboratory of Bioinformatics and Systems Biology, National Research Council Canada, Montreal, QC, Canada.

Fatty acids in crop seeds are a major source for both vegetable oils and industrial applications. Genetic improvement of fatty acid composition and oil content is critical to meet the current and future demands of plant-based renewable seed oils. Addressing this challenge can be approached by network modeling to capture key contributors of seed metabolism and to identify underpinning genetic targets for engineering the traits associated with seed oil composition and content. Here, we present a dynamic model, using an Ordinary Differential Equations model and integrated time-course gene expression data, to describe metabolic networks during seed development. Through perturbation of genes, targets were predicted in seed oil traits. Validation and supporting evidence were obtained for several of these predictions using published reports in the scientific literature. Furthermore, we investigated two predicted targets using omics datasets for both gene expression and metabolites from the seed embryo, and demonstrated the applicability of this network-based model. This work highlights that integration of dynamic gene expression atlases generates informative models which can be explored to dissect metabolic pathways and lead to the identification of causal genes associated with seed oil traits.
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http://dx.doi.org/10.3389/fpls.2021.642938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056077PMC
April 2021

Microstructure-Dependent K Storage in Porous Hard Carbon.

Small 2021 Apr 22:e2100397. Epub 2021 Apr 22.

College of Materials Science and Engineering, Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy, Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology, Hunan University, Changsha, 410082, China.

Hard carbons (HCs) are emerging as promising anodes for potassium-ion batteries (PIBs) due to overwhelming advantages including cost effectiveness and outstanding physicochemical properties. However, the fundamental K storage mechanism in HCs and the key structural parameters that determining K storage behaviors remain unclear and require further exploration. Herein, HC materials with controllable micro/mesopore structures are first synthesized by template-assisted spray pyrolysis technology. Detailed experimental analyses including in situ Raman and in situ electrochemical impedance spectroscopy analysis reveal two different K storage ways in the porous hard carbon (p-HC), e.g., the adsorption mechanism at high potential region and the intercalation mechanism at low potential region. Both are strongly dependent on the evolution of microstructure and significantly affect the electrochemical performance. Specifically, the adequate micropores act as the active sites for efficient K storage and ion-buffering reservoir to relieve the volume expansion, ensuring enhanced specific capacity and good structural stability. The abundant mesopores in the porous structure provide conductive pathways for ion diffusion and/or electrolyte infiltration, endowing fast ionic/electronic transport kinetics. All these together contribute to the high energy density of activated carbon//p-HCs potassium ion hybrid capacitors (74.5 Wh kg , at 184.4 W kg ).
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http://dx.doi.org/10.1002/smll.202100397DOI Listing
April 2021

TWIST2 and the PPAR signaling pathway are important in the progression of nonalcoholic steatohepatitis.

Lipids Health Dis 2021 Apr 20;20(1):39. Epub 2021 Apr 20.

Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, 250014, P. R. China.

Background: To investigate the roles of the transcription factors twist family bHLH transcription factor 1 (TWIST1), twist family bHLH transcription factor 2 (TWIST2), and peroxisome proliferator activated receptor gamma (PPARγ) in the progression of nonalcoholic steatohepatitis.

Methods: The protein levels of TWIST1, TWIST2 and PPARγ were determined in the serum of nonalcoholic fatty liver disease (NAFLD) patients and healthy controls by enzyme-linked immunosorbent assay (ELISA). An in vivo model for fatty liver was established by feeding C57BL/6 J mice a high-fat diet (HFD). An in vitro model of steatosis was established by treating LO-2 cells with oleic acid (OA). RNA sequencing was performed on untreated and OA-treated LO-2 cells followed by TWIST1, TWIST2 and PPARγ gene mRNA levels analysis, Gene Ontology (GO) enrichment and pathway analysis.

Results: The TWIST2 serum protein levels decreased significantly in all fatty liver groups (P < 0.05), while TWIST1 varied. TWIST2 tended to be lower in mice fed an HFD and was significantly lower at 3 months. Similarly, in the in vitro model, the TWIST2 protein level was downregulated significantly at 48 and 72 h after OA treatment. RNA sequencing of LO-2 cells showed an approximately 2.3-fold decrease in TWIST2, with no obvious change in TWIST1 and PPARγ. The PPAR signaling pathway was enriched, with 4 genes upregulated in OA-treated cells (P = 0.0018). The interleukin (IL)-17 and tumor necrosis factor (TNF) signaling pathways were enriched in OA-treated cells.

Conclusions: The results provide evidence that the TWIST2 and PPAR signaling pathways are important in NAFLD and shed light on a potential mechanism of steatosis.
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http://dx.doi.org/10.1186/s12944-021-01458-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059034PMC
April 2021

New insights into long non-coding RNAs in breast cancer: Biological functions and therapeutic prospects.

Exp Mol Pathol 2021 Apr 17;120:104640. Epub 2021 Apr 17.

Key Laboratory for Experimental Teratology of Ministry of Education, Department of Pathology, School of Basic Medical Sciences, CheeLoo College of Medicine, Shandong University, Jinan, Shandong 250012, China; Department of Pathology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China. Electronic address:

Breast cancer (BC) has become one of the most common malignant tumors in the world, seriously endangering women's health and life. However, the underlying molecular mechanisms of BC remain unclear. Over the past decade, long non-coding RNAs (lncRNAs) were gradually discovered and appreciated to play pivotal regulatory role in the progression of BC. It has been demonstrated that lncRNAs are implicated in regulating plenty of biological phenomena including cell proliferation, apoptosis, invasion and metastasis by interacting with DNA, RNA or proteins. In addition to these, the function of lncRNAs in tumor resistance has increasingly attracted more attention. In this review, we summarized the emerging impact of lncRNAs on the occurrence and progression of human BC, specifically focusing on the functions and mechanisms of them, with the aim of exploring the potential value of lncRNAs as oncogenic drivers or tumor suppressors. Furthermore, the potential clinical application of lncRNAs as diagnostic biomarkers and therapeutic targets in BC was also discussed.
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http://dx.doi.org/10.1016/j.yexmp.2021.104640DOI Listing
April 2021

Microscopic Kinetics Pathway of Salt Crystallization in Graphene Nanocapillaries.

Phys Rev Lett 2021 Apr;126(13):136001

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

The fundamental understanding of crystallization, in terms of microscopic kinetic and thermodynamic details, remains a key challenge in the physical sciences. Here, by using in situ graphene liquid cell transmission electron microscopy, we reveal the atomistic mechanism of NaCl crystallization from solutions confined within graphene cells. We find that rock salt NaCl forms with a peculiar hexagonal morphology. We also see the emergence of a transitory graphitelike phase, which may act as an intermediate in a two-step pathway. With the aid of density functional theory calculations, we propose that these observations result from a delicate balance between the substrate-solute interaction and thermodynamics under confinement. Our results highlight the impact of confinement on both the kinetics and thermodynamics of crystallization, offering new insights into heterogeneous crystallization theory and a potential avenue for materials design.
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http://dx.doi.org/10.1103/PhysRevLett.126.136001DOI Listing
April 2021

MiR-573 suppresses cell proliferation, migration and invasion via regulation of E2F3 in pancreatic cancer.

J Cancer 2021 19;12(10):3033-3044. Epub 2021 Mar 19.

Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Pancreatic cancer is among the most lethal malignancies worldwide. In this study, we aimed to determine whether miR-573 could suppress pancreatic cancer cell proliferation, migration, and invasion by targeting E2F3. MiR-573 expression in pancreatic cancer tissues and cell lines was measured using real-time PCR. Target genes of miR-573 were screened using bioinformatics tools and confirmed using dual-luciferase reporter assay and real-time PCR. Pancreatic cancer cells were transfected using an miR-573 mimic or siRNA E2F3. Furthermore, cell proliferation, migration, and invasion were assessed using CCK-8, Edu staining, colony-forming assay, wound healing assay, and transwell assay . The effects of miR-573 were verified using tumor xenografts. Differential expression and prognostic analyses of miR-573 and E2F3 were visualized using the Kaplan‑Meier plotter and GEPIA. We found that the expression of miR-573 was significantly reduced in pancreatic cancer tissues and cell lines. Overexpression of miR-573 obviously suppressed the proliferation, migration, and invasion of pancreatic cancer cells. The Dual-luciferase assay showed that miR-573 could specifically target E2F3. Furthermore, E2F3 was up-regulated in pancreatic cancer tissues and cell lines and E2F3 down-regulation inhibited the proliferation, migration, and invasion of pancreatic cancer cells. The ectopic expression of miR-573 inhibited xenograft tumor growth . Results from the Kaplan-Meier analysis and GEPIA showed that patients with a high level of miR-573 had a significantly reduced risk of death while those with a high level of E2F3 displayed significant correlation with the tumor stage and suffered worse prognosis. MiR-573 could suppress the proliferation, migration, and invasion of pancreatic cancer cells by targeting E2F3, thereby establishing miR-573 as a novel regulator of E2F3 and indicating its critical role in tumorigenesis, especially in pancreatic cancer.
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http://dx.doi.org/10.7150/jca.51147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040892PMC
March 2021

Seeded 2D epitaxy of large-area single-crystal films of the van der Waals semiconductor 2H MoTe.

Science 2021 04;372(6538):195-200

State Key Laboratory for Mesoscopic Physics and Frontiers Science Center for Nano-Optoelectronics, School of Physics, Peking University, Beijing 100871, China.

The integration of two-dimensional (2D) van der Waals semiconductors into silicon electronics technology will require the production of large-scale, uniform, and highly crystalline films. We report a route for synthesizing wafer-scale single-crystalline 2H molybdenum ditelluride (MoTe) semiconductors on an amorphous insulating substrate. In-plane 2D-epitaxy growth by tellurizing was triggered from a deliberately implanted single seed crystal. The resulting single-crystalline film completely covered a 2.5-centimeter wafer with excellent uniformity. The 2H MoTe 2D single-crystalline film can use itself as a template for further rapid epitaxy in a vertical manner. Transistor arrays fabricated with the as-prepared 2H MoTe single crystals exhibited high electrical performance, with excellent uniformity and 100% device yield.
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http://dx.doi.org/10.1126/science.abf5825DOI Listing
April 2021