Publications by authors named "Peng Du"

493 Publications

Non-invasive diagnosis of Crohn's disease based on SERS combined with PCA-SVM.

Anal Methods 2021 Oct 19. Epub 2021 Oct 19.

School of Energy and Power Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Crohn's disease (CD) is an idiopathic chronic inflammatory bowel disease without a cure. Most of the CD patients are firstly diagnosed by invasive endoscopy, and clinical and pathological examinations are further required to confirm the diagnosis. Hence, the development of a non-invasive, rapid and accurate diagnosis method for CD patients is essential. In this study, urine samples from 95 CD patients (including 58 active CD (aCD) patients and 37 inactive CD (iCD) patients) and 48 healthy controls (HC) were investigated by surface-enhanced Raman spectroscopy (SERS). The statistical analysis of the three groups (, CD/HC, aCD/HC and iCD/HC) was performed on the measured data. Principal component analysis (PCA)-support vector machine (SVM) and PCA-linear discriminant analysis (LDA) were then employed to establish classification models to distinguish between patients and HC. For the average SERS spectra of patients and HC, the Raman peaks belonging to lipids, proteins and nucleic acids were stronger in patients than those in HC. It showed that the classification accuracy of CD/HC based on PCA-SVM was higher than that of PCA-LDA (82.5% 69.9%). And the classification accuracy of aCD/HC based on PCA-SVM was higher than that of iCD/HC (86.8% 76.5%). The classification model we established distinguished between aCD and HC with 86.2% sensitivity and 87.5% specificity. It indicates that the metabolic change of patients could be identified by measuring urine with SERS, and aCD and HC could be distinguished more effectively. Our findings are helpful for clinicians to diagnose CD patients and monitor the progress and recurrence of the disease.
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http://dx.doi.org/10.1039/d1ay01377gDOI Listing
October 2021

Correction: Graphene oxide encapsulated by mesoporous silica for intelligent anticorrosive coating: studies on release models and self-healing ability.

Dalton Trans 2021 Oct 19. Epub 2021 Oct 19.

Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.

Correction for 'Graphene oxide encapsulated by mesoporous silica for intelligent anticorrosive coating: studies on release models and self-healing ability' by Peng Du , , 2019, , 13064-13073, DOI: 10.1039/C9DT02454A.
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http://dx.doi.org/10.1039/d1dt90177jDOI Listing
October 2021

Intraoperative serosal extracellular mapping of the human distal colon: a feasibility study.

Biomed Eng Online 2021 Oct 16;20(1):105. Epub 2021 Oct 16.

Department of Surgery, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, 1142, Auckland, New Zealand.

Background: Cyclic motor patterns (CMP) are the predominant motor pattern in the distal colon, and are important in both health and disease. Their origin, mechanism and relation to bioelectrical slow-waves remain incompletely understood. During abdominal surgery, an increase in the CMP occurs in the distal colon. This study aimed to evaluate the feasibility of detecting propagating slow waves and spike waves in the distal human colon through intraoperative, high-resolution (HR), serosal electrical mapping.

Methods: HR electrical recordings were obtained from the distal colon using validated flexible PCB arrays (6 × 16 electrodes; 4 mm inter-electrode spacing; 2.4 cm, 0.3 mm diameter) for up to 15 min. Passive unipolar signals were obtained and analysed.

Results: Eleven patients (33-71 years; 6 females) undergoing colorectal surgery under general anaesthesia (4 with epidurals) were recruited. After artefact removal and comprehensive manual and automated analytics, events consistent with regular propagating activity between 2 and 6 cpm were not identified in any patient. Intermittent clusters of spike-like activities lasting 10-180 s with frequencies of each cluster ranging between 24 and 42 cpm, and an average amplitude of 0.54 ± 0.37 mV were recorded.

Conclusions: Intraoperative colonic serosal mapping in humans is feasible, but unlike in the stomach and small bowel, revealed no regular propagating electrical activity. Although sporadic, synchronous spike-wave events were identifiable. Alternative techniques are required to characterise the mechanisms underlying the hyperactive CMP observed in the intra- and post-operative period.

New Findings: The aim of this study was to assess the feasibility of detecting propagating electrical activity that may correlate to the cyclic motor pattern in the distal human colon through intraoperative, high-resolution, serosal electrical mapping. High-resolution electrical mapping of the human colon revealed no regular propagating activity, but does reveal sporadic spike-wave events. These findings indicate that further research into appropriate techniques is required to identify the mechanism of hyperactive cyclic motor pattern observed in the intra- and post-operative period in humans.
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http://dx.doi.org/10.1186/s12938-021-00944-xDOI Listing
October 2021

SARS-CoV-2 immunity and functional recovery of COVID-19 patients 1-year after infection.

Signal Transduct Target Ther 2021 10 13;6(1):368. Epub 2021 Oct 13.

Department of Nephrology, Center of Nephrology and Urology, Sun Yat-sen University Seventh Hospital, Shenzhen, Guangdong, 518107, China.

The long-term immunity and functional recovery after SARS-CoV-2 infection have implications in preventive measures and patient quality of life. Here we analyzed a prospective cohort of 121 recovered COVID-19 patients from Xiangyang, China at 1-year after diagnosis. Among them, chemiluminescence immunoassay-based screening showed 99% (95% CI, 98-100%) seroprevalence 10-12 months after infection, comparing to 0.8% (95% CI, 0.7-0.9%) in the general population. Total anti-receptor-binding domain (RBD) antibodies remained stable since discharge, while anti-RBD IgG and neutralization levels decreased over time. A predictive model estimates 17% (95% CI, 11-24%) and 87% (95% CI, 80-92%) participants were still 50% protected against detectable and severe re-infection of WT SARS-CoV-2, respectively, while neutralization levels against B.1.1.7 and B.1.351 variants were significantly reduced. All non-severe patients showed normal chest CT and 21% reported COVID-19-related symptoms. In contrast, 53% severe patients had abnormal chest CT, decreased pulmonary function or cardiac involvement and 79% were still symptomatic. Our findings suggest long-lasting immune protection after SARS-CoV-2 infection, while also highlight the risk of immune evasive variants and long-term consequences for COVID-19 survivors.
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http://dx.doi.org/10.1038/s41392-021-00777-zDOI Listing
October 2021

What is the impact of scoliotic correction on postoperative shoulder imbalance in severe and rigid scoliosis.

BMC Musculoskelet Disord 2021 Oct 12;22(1):868. Epub 2021 Oct 12.

Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: Although recent studies have investigated the risk factors for PSI, few studies have focused on the impact of scoliotic correction on postoperative shoulder imbalance (PSI), especially in severe and rigid scoliosis (SRS). The purpose of the study was to study the effect of scoliotic correction on PSI in SRS.

Methods: The preoperative, postoperative, and minimum 2-year follow-up radiographs of 48 consecutive patients with SRS who underwent posterior spinal fusion surgery were evaluated. We regarded radiographic shoulder height (RSH) as a shoulder balance parameter and divided the patients into improved and aggravated groups of PSI from pre- to post-operation and from post-operation to last follow-up, respectively. In addition, patients were divided into nine groups based on the observed changes in PSI after surgery and at follow-up, and the correction rate ratios were calculated among the groups. Independent samples T test and Chi-squared test were performed between the improved and aggravated groups of PSI.

Results: After surgery, the proximal thoracic curve (PTC) flexibility (P = 0.040), correction rate of the main thoracic curve (MTC) (P = 0.010), and Cobb angle of the lumbar curve (LC) (P = 0.037) were significantly higher, while the ratio of the correction rate of the PTC to the MTC (P = 0.042) was smaller in the aggravated group. At follow-up, the improved group had significantly larger PTC flexibility (P = 0.006), larger ratio of the correction rate of PTC to MTC (P = 0.046), a larger ratio correction rate of PTC to LC (P = 0.027), and a smaller correction rate of LC (P = 0.030). The correction rate ratios of the groups after surgery were as follows: negative to negative (N-N) (1.08) > negative to balance (N-B) (0.96) > negative to positive (N-P) (0.67), B-N (1.26) > B-B (0.94) > B-P (0.89), and P-N (0.34) > P-P (0.83). The order of the correction rate ratio at follow-up was as follows: N-N (0.96) > N-B (0.51), B-B (0.97) > B-P (0.90), and P-B (0.87) > P-P (0.84).

Conclusion: Harmonizing the correction rate ratio of the PTC, MTC, and LC should be recommended for intraoperative correction and postoperative compensation of PSI. In addition, greater PTC flexibility plays an important role in the spontaneous correction and compensation of PSI in SRS.
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http://dx.doi.org/10.1186/s12891-021-04763-yDOI Listing
October 2021

Preoperative Endoscopic Activity Predicts the Occurrence of Pouchitis After Ileal Pouch-Anal Anastomosis in Ulcerative Colitis: A Multicenter Retrospective Study in China.

Front Surg 2021 23;8:740349. Epub 2021 Sep 23.

Department of Colorectal Surgery, School of Medicine, Xinhua Hospital, Shanghai Jiaotong University, Shanghai, China.

Pouchitis is the most common long-term complication after ileal pouch-anal anastomosis (IPAA) in patients with ulcerative colitis (UC). Ulcerative colitis endoscopic index of severity (UCEIS) and Mayo endoscopic score (MES) are widely used indices to evaluate endoscopic activity. This study aimed to clarify the predictive value of preoperative endoscopic activity on the occurrence of pouchitis after IPAA. Data of patients with UC who underwent IPAA from January 2008 to January 2020 were collected retrospectively. UCEIS and MES were based on the preoperative colonoscopy findings of two independent endoscopists. A total of 102 patients with a median follow-up of 5 (interquartile range, 2-9) years were included in the study. Among them, 21.6% developed pouchitis. Compared with MES, UCEIS had a stronger correlation with pouchitis disease activity index. UCEIS ≥ 7 had the most significant receiver-operating characteristic (ROC) curve area of 0.747 with a sensitivity of 68.2% and specificity of 81.2% in predicting pouchitis, which outperformed MES of 3 with an ROC area of 0.679 with a sensitivity of 54.5% and specificity of 81.2%. Furthermore, we found that UCEIS ≥ 7 was an independent risk factor for post-IPAA pouchitis [odds ratio (OR), 8.860; 95% CI, 1.969-39.865, < 0.001] with a higher risk than MES of 3 (OR, 5.200; 95% CI, 1.895-14.273; = 0.001). Ulcerative colitis endoscopic index of severity performed better in predicting pouchitis after IPAA than MES. Earlier and more frequent postoperative colonoscopic surveillance should be considered in patients with preoperative UCEIS ≥ 7 to detect the occurrence of pouchitis earlier.
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http://dx.doi.org/10.3389/fsurg.2021.740349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496748PMC
September 2021

METTL3 Is Involved in the Development of Graves' Disease by Inducing SOCS mRNA m6A Modification.

Front Endocrinol (Lausanne) 2021 20;12:666393. Epub 2021 Sep 20.

Department of Endocrinology & Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Objective: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation induced by methyltransferase like 3 (METTL3) with Graves' disease (GD).

Methods: Differently expressed genes (DEG) in GD tissues were identified using microarray analysis and further validated using CD4 T cell microarray of GD tissues and isolated peripheral blood mononuclear cells (PBMCs). Furthermore, expressions of METTL3 targeted genes were detected using METTL3 knock-down experiment in RAW264.7 cells.

Results: High throughput microarrays revealed that METTL3 and SOCS molecules were aberrantly expressed in thyroid tissues and CD4T cells of GD compared to the controls. Bioinformatic analysis was undertaken by searching databases of found genes of the SOCS family that possessed many mRNA m6A modification loci. METTL3 knock-down experiment revealed that expressions of SOCS family members SOCS1, SOCS2, SOCS4, SOCS5, and SOCS6 were increased after METTL3 knock-down.

Conclusions: For the first time, the present study revealed the relationship between m6A modification and GD and indicated that METTL3 may be involved in the development of GD by inducing mRNA m6A methylation modification of SOCS family members.
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http://dx.doi.org/10.3389/fendo.2021.666393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488398PMC
September 2021

NIR emission of lanthanides for ultrasensitive luminescence manometry-Er-activated optical sensor of high pressure.

Dalton Trans 2021 Oct 4. Epub 2021 Oct 4.

Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, 315211 Ningbo, Zhejiang, China.

Pressure is an important physical parameter and hence its monitoring is very important for different industrial and scientific applications. Although commonly used luminescent pressure sensors (ruby-AlO:Cr and SrBO:Sm) allow optical monitoring of pressure in compressed systems (usually in a diamond anvil cell; DAC), their detection resolution is limited by sensitivity, , pressure response in a form of the detected spectral shift. Here we report, a breakthrough in optical pressure sensing by developing an ultra-sensitive NIR pressure sensor (d/d = 1.766 nm GPa). This luminescent manometer is based on the optically active YVO:Yb-Er phosphor material which exhibits the largest spectral shift as a function of pressure compared to other luminescent pressure gauges reported elsewhere. In addition, thanks to the locations of excitation and emission in the NIR range, the developed optical manometer allows high-pressure measurements (without spectral overlapping/interferences) of various luminescent organic and inorganic materials, which are typically excited and can emit in the UV-vis spectral ranges.
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http://dx.doi.org/10.1039/d1dt02681jDOI Listing
October 2021

Intake of Dietary Fiber From Grains and the Risk of Hypertension in Late Midlife Women: Results From the SWAN Study.

Front Nutr 2021 16;8:730205. Epub 2021 Sep 16.

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.

The possible effects of dietary fiber intake on hypertension have not been clarified fully. The association of dietary fiber intake with hypertension risk in midlife women was analyzed in this study. Baseline data were obtained from the Study of Women's Health Across the Nation (SWAN). Smooth curve, linear regression, and logistic regression analyses were performed to investigate the associations of four indices of daily dietary estimate (DDE) of dietary fiber (dietary fiber intake, dietary fiber intake from beans, dietary fiber intake from vegetables/fruit, and dietary fiber intake from grains) with blood pressure in midlife women. For this research purpose, diastolic blood pressure (DBP) ≥90 mmHg was defined as diastolic hypertension, and systolic blood pressure SBP) ≥140 mmHg was defined as systolic hypertension. This study included 2,519 participants with an average age of 46. The smooth curve showed approximate negative correlations between three fiber indices (DDE dietary fiber, DDE fiber from vegetables/fruit, and DDE fiber from grains) and blood pressure, including DBP and SBP (all < 0.005). There were also approximate negative correlations between two fiber indices (DDE dietary fiber and DDE fiber from grains) and the risk of diastolic hypertension and systolic hypertension (all < 0.05). Furthermore, multiple linear regression analysis suggested that DDE dietary fiber (Sβ = -0.057, 95% CI -0.194 - -0.012, = 0.027), DDE fiber from vegetables/fruit (Sβ = -0.046, 95% CI -0.263 - -0.007, = 0.039), and DDE fiber from grains (Sβ = -0.073, 95% CI -0.600 - -0.099, = 0.006, Model 4) were still negatively correlated with DBP after adjusting for confounding factors. Only DDE fiber from grains was independently and negatively associated with SBP (Sβ = -0.060, 95% CI -0.846 - -0.093, = 0.015) after these same confounding factors were adjusted for. Importantly, multiple logistic regression analysis suggested that only higher DDE fiber from grains was independently associated with a reduced risk of diastolic hypertension (OR = 0.848, 95% CI 0.770-0.934, = 0.001, Model 4) and systolic hypertension (OR = 0.906, 95% CI 0.826-0.993, = 0.034, Model 4) after the adjustments were made for confounding factors. We found that dietary fiber intake, especially DDE fiber from grains, contributes to a lower risk of systolic hypertension and diastolic hypertension in midlife women.
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http://dx.doi.org/10.3389/fnut.2021.730205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481373PMC
September 2021

Effects of anatomical variations of the stomach on body-surface gastric mapping investigated using a large population-based multiscale simulation approach.

IEEE Trans Biomed Eng 2021 Sep 29;PP. Epub 2021 Sep 29.

The contractions of the stomach are governed by bioelectrical slow waves that can be detected non-invasively from the body-surface. Diagnosis of gastric motility disorders remains challenging due to the limited information provided by symptoms and tests, including standard electrogastrography (EGG). Body-surface gastric mapping (BSGM) is a novel technique that measures the resultant body-surface potentials using an array of multiple cutaneous electrodes. However, there is no established protocol to guide the placement of the mapping array and to account for the effects of biodiversity on the interpretation of gastric BSGM data. This study aims to quantify the effect of anatomical variation of the stomach on body surface potentials. To this end, 93 subject specific models of the stomach and torso were developed. Anatomical models were developed based on data obtained from the Cancer Imaging Archive. For each subject a set of points were created to model general anatomy the stomach and the torso, using a finite element mesh. A bidomain model was used to simulate the gastric slow waves in the antegrade wave (AW) direction and formation of colliding waves (CW). The resultant dipole was calculated, and a forward modeling approach was employed to simulate body-surface potentials. Simulated data were sampled from a 55 array of electrodes from the body-surface and compared between AW and CW cases. Anatomical parameters such as the Euclidean distance from the xiphoid process (8.6 2.2 cm), orientation relative to the axial plane (195 20.0) were quantified. Electrophysiological simulations of AW and CW were both correlated to specific metrics derived from BSGM signals. In general, the maximum amplitude () and orientation () of the signals provided consistent separation of AW and CW. The findings of this study will aid gastric BSGM electrode array design and placement protocol in clinical practices.
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http://dx.doi.org/10.1109/TBME.2021.3116287DOI Listing
September 2021

11,11,12,12-tetracyano-9,10-anthraquinonedimethane as a high potential and sustainable cathode for organic potassium-ion batteries.

J Colloid Interface Sci 2021 Sep 2;607(Pt 2):1173-1179. Epub 2021 Sep 2.

Institute of Materials Science & Devices, School of Material Science and Engineering, Suzhou University of Science and Technology, Suzhou 215000, PR China; Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, PR China; Institute of Advanced Cross-field Science, College of Life Science, Qingdao University, Qingdao 200671, PR China. Electronic address:

We fabricated a potassium-ion battery by using 11,11,12,12-tetracyano-9,10-anthraquinonedimethane (TCAQ) as the cathode for the first time. Owing to the unique molecular structure and configuration of ionic liquid electrolytes, TCAQ shows a high redox potential of 2.6 V vs. K/K while delivering a capacity of 88 mAh g at a current density of 17 mA g and a capacity retention of 61% after 50 cycles. The mechanism of the reaction of TCAQ with K was investigated. The results prove that TCAQ holds great promise for broad applications in potassium-ion batteries while revealing new scientific insights into K-organic cathode batteries.
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http://dx.doi.org/10.1016/j.jcis.2021.08.204DOI Listing
September 2021

CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta, alpha, beta, and gamma.

Signal Transduct Target Ther 2021 09 25;6(1):347. Epub 2021 Sep 25.

National Translational Science Center for Molecular Medicine & Department of Cell Biology, Fourth Military Medical University, Xi'an, 710032, China.

SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape, compromising the effectiveness of existing vaccines and neutralizing antibodies. An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants. Here, we identified CD147 as a universal receptor for SARS-CoV-2 and its variants. Meanwhile, Meplazeumab, a humanized anti-CD147 antibody, could block cellular entry of SARS-CoV-2 and its variants-alpha, beta, gamma, and delta, with inhibition rates of 68.7, 75.7, 52.1, 52.1, and 62.3% at 60 μg/ml, respectively. Furthermore, humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants, alpha and beta. When infected, these mice developed exudative alveolar pneumonia, featured by immune responses involving alveoli-infiltrated macrophages, neutrophils, and lymphocytes and activation of IL-17 signaling pathway. Mechanistically, we proposed that severe COVID-19-related cytokine storm is induced by a "spike protein-CD147-CyPA signaling axis": Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway, which further induced expression of cyclophilin A (CyPA); CyPA reciprocally bound to CD147 and triggered MAPK pathway. Consequently, the MAPK pathway regulated the expression of cytokines and chemokines, which promoted the development of cytokine storm. Importantly, Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants. Therefore, our findings provided a new perspective for severe COVID-19-related pathogenesis. Furthermore, the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment.
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http://dx.doi.org/10.1038/s41392-021-00760-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464593PMC
September 2021

The Gastric Conduction System in Health and Disease: A Translational Review.

Am J Physiol Gastrointest Liver Physiol 2021 09 22. Epub 2021 Sep 22.

Farncombe Family Digestive Health Research Institute, Department of Medicine, McMaster University, Canada.

Gastric peristalsis is critically dependent on an underlying electrical conduction system. Recent years have witnessed substantial progress in clarifying the operations of this system, including its pacemaking units, its cellular architecture, and slow wave propagation patterns. Advanced techniques have been developed for assessing its functions at high spatiotemporal resolutions. This review synthesizes and evaluates this progress, with a focus on human and translational physiology. A current conception of the initiation and conduction of slow wave activity in the human stomach is provided first, followed by a detailed discussion of its organisation at the cellular and tissue level. Particular emphasis is then given to how gastric electrical disorders may contribute to disease states. Gastric dysfunction continues to grow in their prevalence and impact, and while gastric dysrhythmia is established as a clear and pervasive feature in several major gastric disorders, its role in explaining pathophysiology and informing therapy is still emerging. New insights from high-resolution gastric mapping are evaluated, together with historical data from electrogastrography, and the physiological relevance of emerging biomarkers from body surface mapping such as retrograde propagating slow waves. Knowledge gaps requiring further physiological research are highlighted.
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http://dx.doi.org/10.1152/ajpgi.00065.2021DOI Listing
September 2021

PRMT4 promotes hepatocellular carcinoma progression by activating AKT/mTOR signaling and indicates poor prognosis.

Int J Med Sci 2021 27;18(15):3588-3598. Epub 2021 Aug 27.

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.

: Protein arginine methyltransferase 4 (PRMT4) has been reported to play a role in several common cancers; however, the function and mechanism of PRMT4 in hepatocellular carcinoma (HCC) are not fully understood. This study aimed to investigate the role and mechanism of PRMT4 in the progression of HCC. PRMT4 expression and clinicopathological characteristics were investigated using an HCC tissue microarray (TMA) consisting of 140 patient samples analyzed by immunohistochemistry. CCK-8, crystal violet and Transwell assays were used to determine cell proliferation, colony formation, migration, and invasion of HCC cell lines in which PRMT4 was overexpressed or downregulated. The underlying mechanism of PRMT4 function was explored by Western blot assays. PRMT4 was highly expressed in HCC tumor tissues compared to adjacent nontumor tissues. PRMT4 expression was significantly associated with alpha-fetoprotein levels, tumor size, satellite nodules, and microvascular invasion. Patients with higher PRMT4 expression had a shorter survival time and higher recurrence rate. Functional studies demonstrated that PRMT4 overexpression promoted HCC cell proliferation, migration, and invasion , while knocking down PRMT4 inhibited these malignant behaviors. Additional results revealed that PRMT4 promoted the progression of HCC cells via activation of the AKT/mTOR signaling pathway. Furthermore, inhibition of the AKT/mTOR signaling by MK2206 or rapamycin significantly attenuated PRMT4-mediated malignant phenotypes. This study suggests that PRMT4 may promote the progression of HCC cells by activating the AKT/mTOR signaling pathway, which may be a valuable biomarker and potential target for HCC.
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http://dx.doi.org/10.7150/ijms.62467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436100PMC
August 2021

Relationship Between Serum Albumin and Risk of Atrial Fibrillation: A Dose-Response Meta-Analysis.

Front Nutr 2021 18;8:728353. Epub 2021 Aug 18.

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.

The dose-response association between serum albumin and atrial fibrillation is not well known. This study aims to assess the relationship between albumin and atrial fibrillation and the potential dose-response effect. Studies reported that the serum albumin and AF were identified by searching the EMBASE, PubMed, and Cochrane Library databases. The potential dose-response effect was performed by using a stage robust error meta-regression. Nine studies were included with a total of 32,130 individuals. Patients with high albumin level were associated with a decreased risk of atrial fibrillation compared with patients with low serum albumin (OR[odds ratio]: 0.62, 95% CI [0.44, 0.89]; = 76%; = 0.009). In the dose-response analysis, for each 10 g/L increase in serum albumin level, the risk of atrial fibrillation decreased by 36% (95% CI: 0.51-0.81, = 87%, < 0.001). Furthermore, a significant negative linear relationship between serum albumin and the risk of atrial fibrillation ( = 0.33) was found. Our dose-response meta-analysis suggests that low serum albumin level is associated with an increased risk of atrial fibrillation. Further studies are needed to explore the effect of induction of elevated serum albumin levels on the prevention of atrial fibrillation.
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http://dx.doi.org/10.3389/fnut.2021.728353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418186PMC
August 2021

Systemic Proteomic Analysis Reveals Distinct Exosomal Protein Profiles in Rheumatoid Arthritis.

J Immunol Res 2021 18;2021:9421720. Epub 2021 Aug 18.

Department of Endocrinology & Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, China.

Objective: Rheumatoid arthritis (RA) is a complex disease with unknown pathogenesis. In recent years, fewer have paid attention to the broad spectrum of systemic markers of RA. The aim of this study was to identify exosomal candidate proteins in the pathogenesis of RA.

Methods: Totally, 12 specimens of plasma from 6 RA patients and 6 age- and gender-matched controls from the Chinese population were obtained for nanoscale liquid chromatography coupled to tandem mass spectrometry (nano-LC-MS/MS) analysis to identify exosomal profiles.

Results: A total of 278 exosomal proteins were detected. Among them, 32 proteins were significantly upregulated (FC ≥ 2.0 and < 0.05) and 5 proteins were downregulated (FC ≤ 0.5 and < 0.05). Bioinformatics analysis revealed that transthyretin (TTR), angiotensinogen (AGT), lipopolysaccharide-binding protein (LBP), monocyte differentiation antigen CD14 (CD14), cartilage oligomeric matrix protein (COMP), serum amyloid P (SAP/APCS), and tenascin (TNC) can interact with each other. Subsequently, these cross-linked proteins may be mainly involved in the inflammatory-related pathways to mediate the onset of RA. Noteworthy, the LBP/CD14 complex can promote the expression of IL-8 and TNF-, eventually leading to the development of RA.

Conclusions: Our findings suggest distinct plasmatic exosomal protein profiles in RA patients. These proteins not only take important parts in the vicious circle in the pathogenic process of RA but also serve as novel biomarkers in RA diagnosis and prognosis.
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http://dx.doi.org/10.1155/2021/9421720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390169PMC
August 2021

microRNA-199a downregulation alleviates hyperuricemic nephropathy the PPARγ/β-catenin axis.

J Recept Signal Transduct Res 2021 Aug 25:1-9. Epub 2021 Aug 25.

Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China.

Hyperuricemia always develops into hyperuricemic nephropathy (HN). The role of microRNA (miR) in HN is less studied. We aimed to discuss the role of miR-199a in HN. The expression of miR-199a and PPARγ in renal tissues of HN rats was detected. The targeting relation between miR-199a and PPARγ was verified. The contents of SCr, UA, BUN, and mALB, renal injury-relevant biomarkers were detected, and the pathological changes of renal tissue and renal interstitial fibrosis were observed by histological staining. After miR-199a and PPARγ knockdown, the contents of SCr, BUN, and mALB and renal interstitial fibrosis were estimated. Collectively, overexpression of miR-199a aggravated the renal injury in HN rats. By contrast, inhibition of miR-199a weakened renal injury, as evidenced by decreased contents of SCr, UA, BUN, and mALB, and mitigated renal interstitial fibrosis. miR-199a targeted PPARγ. Silencing of PPARγ upregulated the levels of downstream genes of β-catenin and the contents of SCr, UA, BUN, and mALB and deteriorated renal interstitial fibrosis. Moreover, the silencing of PPARγ blocked the alleviative effects of miR-199a inhibitor on the renal injury. Overall, miR-199a targets PPARγ and activates the β-catenin pathway, thus aggravating HN, which might provide a future target for the treatment of HN.
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http://dx.doi.org/10.1080/10799893.2021.1967392DOI Listing
August 2021

Long Noncoding RNA OR7E156P/miR-143/HIF1A Axis Modulates the Malignant Behaviors of Glioma Cell and Tumor Growth in Mice.

Front Oncol 2021 6;11:690213. Epub 2021 Aug 6.

Department of Neurosurgery, Xiangya Hospital, The Central South University (CSU), Changsha, China.

Gliomas are characterized by high incidence, recurrence and mortality all of which are significant challenges to efficacious clinical treatment. The hypoxic microenvironment in the inner core and intermediate layer of the tumor mass of gliomas is a critical contributor to glioma pathogenesis. In this study, we identified an upregulated lncRNA, OR7E156P, in glioma was identified. The silencing of OR7E156P inhibited cell invasion and DNA synthesis and tumor growth . OR7E156P was intricately linked to the HIF1A pathway. Hypoxia could induce OR7E156P expression, whereas OR7E156P silencing decreased HIF1A protein levels under hypoxic conditions. Hypoxia promoted glioma cell invasion and DNA synthesis, and HUVEC tube formation, whereas OR7E156P silencing partially reversed the cellular effects of hypoxia. HIF1A overexpression promoted, whereas OR7E156P silencing inhibited tumor growth; the inhibitory effects of OR7E156P silencing on tumor growth were partially reversed by HIF1A overexpression. miR-143 directly targeted OR7E156P and HIF1A, respectively. miR-143 inhibition increased HIF1A protein levels, promoted glioma cell invasion and DNA synthesis. Moreover, they enhanced HUVEC tube formation, whereas OR7E156P silencing partially reversed the cellular effects of miR-143 inhibition. HIF1A targeted the promoter region of miR-143 and inhibited miR-143 expression. Altogether a regulatory axis consisting of OR7E156P, miR-143, and HIF1A, was identified which is deregulated in glioma, and the process of the OR7E156P/miR-143/HIF1A axis modulating glioma cell invasion through ZEB1 and HUVEC tube formation through VEGF was demonstrated.
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http://dx.doi.org/10.3389/fonc.2021.690213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377393PMC
August 2021

Comparison of Survival Outcomes of Chemotherapy Plus Surgery vs Chemotherapy Alone for Patients With Isolated Liver Metastases From Gallbladder Carcinoma.

Am Surg 2021 Aug 12:31348211038563. Epub 2021 Aug 12.

Department of General Surgery, 117970The First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Chemotherapy is the standard of treatment for metastatic gallbladder carcinoma (GBC). It is unclear whether chemotherapy plus surgery improves the survival outcomes of patients with isolated liver metastases from GBC. We aimed to investigate the survival benefits of chemotherapy plus surgery in GBC with isolated liver metastases compared to those of chemotherapy alone.

Methods: We identified 406 patients with isolated liver metastases from GBC who underwent chemotherapy alone or chemotherapy plus surgery between 2010 and 2015 from Surveillance, Epidemiology, and End Results. Patients were divided into 3 subgroups: group I, chemotherapy alone (n = 263); group II, chemotherapy combined with cholecystectomy (n = 116); and group III, chemotherapy combined with cholecystectomy plus hepatectomy (n = 27). The cancer-specific survival and overall survival were evaluated.

Results: Compared with group I, group II revealed a longer survival time (P < .001). In addition, the survival time of the group III was also prolonged (P < .001). Multivariate cox analysis showed that treatment strategy was an independent prognostic factor.

Conclusion: Chemotherapy combined with resection of the primary tumor plus or not plus resection of the metastatic lesions may be beneficial in GBC with isolated liver metastases.
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http://dx.doi.org/10.1177/00031348211038563DOI Listing
August 2021

Long non-coding RNA VCAN-AS1 promotes the malignant behaviors of breast cancer by regulating the miR-106a-5p-mediated STAT3/HIF-1α pathway.

Bioengineered 2021 12;12(1):5028-5044

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

An accumulating number of studies have found that long noncoding RNAs (lncRNAs) participate in breast cancer (BC) development. LncRNA VCAN-AS1, a novel lncRNA, has been confirmed to regulate the progression of gastric cancer, while its role in BC is elusive. Here, our results illustrate that VCAN-AS1 is overexpressed in BC tissues and cells, while miR-106a-5p was downregulated and negatively correlated with VCAN-AS1. In addition, high VCAN-AS1 expression and low miR-106a-5p expression were closely correlated with poor overall survival in BC patients. Functional experiments confirmed that VCAN-AS1 overexpression notably accelerated BC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) and enhanced tumor cell growth while also suppressing cell apoptosis. However, overexpression of miR-106a-5p had the opposite effects. In addition, rescue experiments confirmed that overexpression of VCAN-AS1 inhibited the tumor-suppressive effects mediated by miR-106a-5p. Mechanistically, through bioinformatics analysis, we found that VCAN-AS1 functions as a competitive endogenous RNA (ceRNA) of miR-106a-5p, which targets the 3' untranslated region (UTR) of signal transducer and activator of transcription 3 (STAT3). Further experiments indicated that miR-106a-5p downregulated the STAT3/hypoxia-inducible factor-1alpha (HIF-1α) pathway, while activating the STAT3 pathway reversed miR-106a-5p-mediated antitumor effects. Collectively, our data suggest that VCAN-AS1 is upregulated in breast cancer and promotes its progression by regulating the miR-106a-5p-mediated STAT3/HIF-1α pathway. This study provides a new target for BC therapy.
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http://dx.doi.org/10.1080/21655979.2021.1960774DOI Listing
December 2021

Study Progress of Noninvasive Imaging and Radiomics for Decoding the Phenotypes and Recurrence Risk of Bladder Cancer.

Front Oncol 2021 15;11:704039. Epub 2021 Jul 15.

School of Biomedical Engineering, Air Force Medical University, Xi'an, China.

Urinary bladder cancer (BCa) is a highly prevalent disease among aged males. Precise diagnosis of tumor phenotypes and recurrence risk is of vital importance in the clinical management of BCa. Although imaging modalities such as CT and multiparametric MRI have played an essential role in the noninvasive diagnosis and prognosis of BCa, radiomics has also shown great potential in the precise diagnosis of BCa and preoperative prediction of the recurrence risk. Radiomics-empowered image interpretation can amplify the differences in tumor heterogeneity between different phenotypes, i.e., high-grade low-grade, early-stage vs. advanced-stage, and nonmuscle-invasive muscle-invasive. With a multimodal radiomics strategy, the recurrence risk of BCa can be preoperatively predicted, providing critical information for the clinical decision making. We thus reviewed the rapid progress in the field of medical imaging empowered by the radiomics for decoding the phenotype and recurrence risk of BCa during the past 20 years, summarizing the entire pipeline of the radiomics strategy for the definition of BCa phenotype and recurrence risk including region of interest definition, radiomics feature extraction, tumor phenotype prediction and recurrence risk stratification. We particularly focus on current pitfalls, challenges and opportunities to promote massive clinical applications of radiomics pipeline in the near future.
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http://dx.doi.org/10.3389/fonc.2021.704039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321511PMC
July 2021

High-Temperature and Large-Polarization Ferroelectric with Second Harmonic Generation Response in a Novel Crown Ether Clathrate.

Chemistry 2021 Sep 26;27(54):13575-13581. Epub 2021 Aug 26.

Faculty of Materials Metallurgy and Chemistry, Jiangxi University of Science and Technology, Ganzhou, 341000, P. R. China.

Molecular ferroelectrics of high-temperature reversible phase transitions are very rare and have attracted increasing attention in recent years. In this paper is described the successful synthesis of a novel high-temperature host-guest inclusion ferroelectric: [(C H NF )(18-crown-6)][BF ] (1) that shows a pair of reversible peaks at 348 K (heating) and 331 K (cooling) with a heat hysteresis about 17 K by differential scanning calorimetry measurements, thus indicating that 1 undergoes a reversible structural phase transition. Variable-temperature PXRD and temperature-dependent dielectric measurements further prove the phase-transition behavior of 1. The second harmonic response demonstrates that 1 belongs to a non-centrosymmetric space group at room temperature and is a good nonlinear optical material. In its semiconducting properties, 1 shows a wide optical band gap of about 4.43 eV that comes chiefly from the C, H and O atoms of the crystals. In particular, the ferroelectric measurements of 1 exhibit a typical polarization-electric hysteresis loop with a large spontaneous polarization (P ) of about 4.06 μC/cm . This finding offers an alternative pathway for designing new ferroelectric-dielectric and nonlinear optical materials and related physical properties in organic-inorganic and other hybrid crystals.
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http://dx.doi.org/10.1002/chem.202101707DOI Listing
September 2021

Assessment of infectivity and the impact on neutralizing activity of immune sera of the COVID-19 variant, CAL.20C.

Signal Transduct Target Ther 2021 07 27;6(1):285. Epub 2021 Jul 27.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

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http://dx.doi.org/10.1038/s41392-021-00695-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313410PMC
July 2021

N501Y mutation imparts cross-species transmission of SARS-CoV-2 to mice by enhancing receptor binding.

Signal Transduct Target Ther 2021 07 27;6(1):284. Epub 2021 Jul 27.

Laboratory of Cell Engineering, Institute of Biotechnology; Research Unit of Cell Death Mechanism, Chinese Academy of Medical Sciences, 2020RU009, Beijing, China.

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http://dx.doi.org/10.1038/s41392-021-00704-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313414PMC
July 2021

Hypoxia-inducible factor-1alpha protects the liver against ischemia-reperfusion injury by regulating the A2B adenosine receptor.

Bioengineered 2021 12;12(1):3737-3752

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

Hepatic ischemia-reperfusion injury (IRI) is an inevitable complication associated with liver surgical procedures, and its pathological process remains elusive. Therefore, the present study investigated the role and mechanism of hypoxia-inducible factor-1alpha (HIF-1α) in hepatic IRI. Here, we constructed rat models with hepatic IRI and BRL-3A cell models with hypoxia/reoxygenation (H/R) insult. The extent of liver injury was assayed by measuring serum ALT/AST levels and performing H&E staining; the levels of SOD, MDA, MPO, IL-6 and TNF-α were determined using commercial kits; apoptosis was detected using the TUNEL assay and flow cytometry; and the expression of HIF-1α/A2BAR signaling-related molecules and apoptosis-associated indicators was detected using Western blotting or qRT-PCR. The expression level of HIF-1α was significantly upregulated in the liver of rats subjected to IRI, as well as in BRL-3A cells treated with H/R. HIF-1α overexpression exerted a protective effect on hepatic IRI or H/R insult by reducing serum aminotransferase levels and hepatic necrosis, inhibiting inflammation and apoptosis of hepatocytes, and alleviating oxidative stress. In contrast, inhibition of HIF-1α expression exacerbated hepatic injury induced by IR or H/R. Mechanistically, the expression level of A2BAR was markedly increased during hepatic IRI or H/R insult. Moreover, A2BAR expression increased with HIF-1α upregulation and decreased with HIF-1α downregulation. Importantly, inhibition of A2BAR signaling abolished HIF-1α overexpression-mediated hepatoprotection. Taken together, HIF-1α exerts protective effects on hepatic IRI by attenuating liver necrosis, the inflammatory response, oxidative stress and apoptosis, and its mechanism may be related to the upregulation of A2BAR signaling.
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http://dx.doi.org/10.1080/21655979.2021.1953217DOI Listing
December 2021

Acts as a Novel Biomarker in the Diagnosis of Patients with Hepatocellular Carcinoma.

Cancer Biother Radiopharm 2021 Jul 20. Epub 2021 Jul 20.

Department of Interventional Radiology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

MicroRNA-424 () is proved to be a tumor suppressor against many malignancies, including hepatocellular carcinoma (HCC). Nevertheless, its role in diagnosing HCC remained poorly understood. The authors' research investigated diagnostic value of serum in HCC. Relative expression levels of serum in HCC patients and healthy individuals were measured via quantitative real-time polymerase chain reaction. χ test was applied to analyze the correlation between expression and clinical features of HCC cases. Diagnostic value was estimated via plotting a receiver operating characteristic (ROC) curve. Serum expression was obviously downregulated in HCC cases in comparison to healthy persons ( < 0.001). expression presented strong correlation with tumor node metastasis stage ( = 0.022), Barcelona Clinic Liver Cancer stage ( < 0.001), metastasis ( = 0.037), and vein invasion ( = 0.033). ROC curve analysis manifested an area under the curve of 0.768 with a sensitivity of 75.0% and a specificity of 72.4%, suggesting that serum had high diagnostic value in HCC patients. The data suggest that serum may represent a biomarker in early detection of HCC.
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http://dx.doi.org/10.1089/cbr.2020.4141DOI Listing
July 2021

Ultrafast heating to boost the electrocatalytic activity of iridium towards oxygen evolution reaction.

Chem Commun (Camb) 2021 Aug;57(63):7830-7833

State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, P. R. China.

Efficient electrocatalysts are in great demand for renewable energy storage systems. Herein, we propose an ultrafast heating strategy to fabricate an efficient Ir/CP-UH catalyst for the oxygen evolution reaction (OER). Experimental results demonstrated that the ultrasmall Ir nanoparticles (≈1-3 nm) and clusters (<1 nm) were highly dispersed on the carbon paper support after a short thermal shock (∼5 s). The catalyst showed a low overpotential of 260 mV at 10 mA cm-2 and remarkable mass activity of about 13.8 times higher than that of the current state-of-the-art commercial Ir/C catalyst. This ultrafast heating strategy can also be applied to other catalyst systems for OER and other electrochemical reactions.
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http://dx.doi.org/10.1039/d1cc01999fDOI Listing
August 2021

Analysing wastewater to estimate fentanyl and tramadol use in major Chinese cities.

Sci Total Environ 2021 Nov 4;795:148838. Epub 2021 Jul 4.

Queensland Alliance for Environmental Health Sciences (QAEHS), The University of Queensland, Brisbane, Queensland 4102, Australia.

The misuse of fentanyl and more recently tramadol in the population has caused an opioid crisis in several countries and drawn much public attention worldwide. However, there is a gap of information on the potential misuse of fentanyl and tramadol in China. This study aims to fill this gap by analysing fentanyl and tramadol in wastewater of major cities across China to estimate their use. Wastewater samples were collected from 30 cities located across all seven geographic regions of China, from 2016 to 2019. Fentanyl was detected in only a few samples, suggesting a low prevalence of this potent opioid drug in China. Meanwhile, tramadol was found in most samples with concentrations ranging up to 186 ng/L. The per capita daily consumption of tramadol estimated from wastewater across China ranged from 6 mg/d/1000 in. to 213 mg/d/1000 inh. The consumption of tramadol seems to be similar among all the days of the week. Tramadol use is overall higher in Northeast China than in other regions, which is different from heroin, another popular opioid in China. Temporally, there is a significant decrease in tramadol use in major cities of China from 2016 to 2019. The results of our study suggested that tramadol use in China was predominantly from pharmaceutical prescription and not as prevalent as in other countries.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148838DOI Listing
November 2021

Species Tree Inference Methods Intended to Deal with Incomplete Lineage Sorting Are Robust to the Presence of Paralogs.

Syst Biol 2021 Jul 10. Epub 2021 Jul 10.

Department of Computer Science, Rice University, 6100 Main Street, Houston, TX 77005, USA.

Many recent phylogenetic methods have focused on accurately inferring species trees when there is gene tree discordance due to incomplete lineage sorting (ILS). For almost all of these methods, and for phylogenetic methods in general, the data for each locus is assumed to consist of orthologous, single-copy sequences. Loci that are present in more than a single copy in any of the studied genomes are excluded from the data. These steps greatly reduce the number of loci available for analysis. The question we seek to answer in this study is: What happens if one runs such species tree inference methods on data where paralogy is present, in addition to or without ILS being present? Through simulation studies and analyses of two large biological data sets, we show that running such methods on data with paralogs can still provide accurate results. We use multiple different methods, some of which are based directly on the multispecies coalescent (MSC) model, and some of which have been proven to be statistically consistent under it. We also treat the paralogous loci in multiple ways: from explicitly denoting them as paralogs, to randomly selecting one copy per species. In all cases the inferred species trees are as accurate as equivalent analyses using single-copy orthologs. Our results have significant implications for the use of ILS-aware phylogenomic analyses, demonstrating that they do not have to be restricted to single-copy loci. This will greatly increase the amount of data that can be used for phylogenetic inference.
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http://dx.doi.org/10.1093/sysbio/syab056DOI Listing
July 2021

T cell receptor revision and immune repertoire changes in autoimmune diseases.

Int Rev Immunol 2021 Jul 9:1-17. Epub 2021 Jul 9.

Department of Endocrinology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Autoimmune disease (AID) is a condition in which the immune system breaks down and starts to attack the body. Some common AIDs include systemic lupus erythematosus, rheumatoid arthritis, type 1 diabetes mellitus and so forth. The changes in T-cell receptor (TCR) repertoire have been found in several autoimmune diseases, and may be responsible for the breakdown of peripheral immune tolerance. In this review, we discussed the processes of TCR revision in peripheral immune environment, the changes in TCR repertoire that occurred in various AIDs, and the specifically expanded T cell clones. We hope our discussion can provide insights for the future studies, helping with the discovery of disease biomarkers and expanding the strategies of immune-targeted therapy. HighlightsRestricted TCR repertoire and biased TCR-usage are found in a variety of AIDs.TCR repertoire shows tissue specificity in a variety of AID diseases.The relationship between TCR repertoire diversity and disease activity is still controversial in AIDs.Dominant TCR clonotypes may help to discover new disease biomarkers and expand the strategies of immune-targeted therapy.
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http://dx.doi.org/10.1080/08830185.2021.1929954DOI Listing
July 2021
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