Publications by authors named "Peng Cui"

330 Publications

2D Heterostructured Nanofluidic Channels for Enhanced Desalination Performance of Graphene Oxide Membranes.

ACS Nano 2021 Apr 6. Epub 2021 Apr 6.

School of Chemistry and Material Science, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

The two-dimensional (2D) lamellar membrane assembly technique shows substantial potential for sustainable desalination applications. However, the relatively wide and size-variable channels of 2D membranes in aqueous solution result in inferior salt rejections. Here we show the establishment of nanofluidic heterostructured channels in graphene oxide (GO) membranes by adding g-CN sheets into GO interlamination. Benefiting from the presence of stable and sub-nanometer wide (0.42 nm) GO/g-CN channels, the GO/g-CN membrane exhibits salt rejections of ∼90% with water permeances of above 30 L h m bar, while the pure GO membrane only has salt rejections of below 30% accompanied by water permeances of below 4 L h m bar. Combining experimental and theoretical investigations, size exclusion has proved to be the dominating mechanism for high rejections, and the ultralow friction water flow along g-CN sheets is responsible for permeation enhancements. Importantly, the GO/g-CN membrane shows promising long-term, antioxidation, and antipressure stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c01105DOI Listing
April 2021

Pyridyldiimine macrocyclic ligands: Influences of template ion, linker length and imine substitution on ligand synthesis, structure and redox properties.

Polyhedron 2021 Apr 30;198. Epub 2021 Jan 30.

University of Pennsylvania for this work.

A series of 2,6-diiminopyridine-derived macrocyclic ligands have been synthesized via [2+2] condensation around alkaline earth metal triflate salts. The inclusion of a -butyl group at the 4-position of the pyridine ring of the macrocyclic synthons results in macrocyclic complexes that are soluble in common organic solvents, thereby enabling a systematic comparison of the physical properties of the complexes by NMR spectroscopy, mass spectrometry, solution-phase UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray crystallography. Solid-state structures determined crystallographically demonstrate increased twisting in the ligand, concurrent with either a decrease in ion size or an increase in macrocycle ring size (18, 20, or 22 membered rings). The degree of folding and twisting within the macrocycle can be quantified using parameters derived from the N-M-N bond angle and the relative orientation of the pyridinediimine (PDI) and pyridinedialdimine (PDAI) fragments to each other within the solid state structures. Cyclic voltammetry and UV-Vis spectroscopy were used to compare the relative energies of the imine π* orbital of the redox active PDI and PDAI components in the macrocycle when coordinated to redox inactive metals. Both methods indicate the change from a methyl to hydrogen substitution on the imine carbon lowers the energy of the ligand π* system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.poly.2021.115044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990114PMC
April 2021

Long-term neurodevelopment outcomes of hand, foot and mouth disease inpatients infected with EV-A71 or CV-A16, a retrospective cohort study.

Emerg Microbes Infect 2021 Dec;10(1):545-554

School of Public Health, Fudan University, Key Laboratory of Public Health Safety, Ministry of Education, Shanghai, People's Republic of China.

Hand, foot and mouth disease (HFMD) is a common infectious disease in western Asia area and the full range of the long-term sequelae of HFMD remains poorly described. We conducted a retrospective hospital-based cohort study of HFMD patients with central nervous system (CNS) complications caused by EV-A71 or CV-A16 between 2010 and 2016. Patients were classified into three groups, including CNS only, autonomic nervous system (ANS) dysregulation, and cardiorespiratory failure. Neurologic examination, neurodevelopmental assessments, Magnetic Resonance Imaging (MRI) and lung function, were performed at follow up. Of the 176 patients followed up, 24 suffered CNS only, 133 ANS dysregulation, and 19 cardiorespiratory failure. Median follow-up period was 4.3 years (range [1.4-8.3]). The rate of neurological abnormalities was 25% (43 of 171) at discharge and 10% (17 of 171) at follow-up. The rates of poor outcome were significantly different between the three groups of complications in motor (28%, 38%, 71%) domain (p=0.020), but not for cognitive (20%, 24%, 35%), language (25%, 36%, 41%) and adaptive (24%, 16%, 26%) domains ( = 0.537,  = 0.551,  = 0.403). For children with ventilated during hospitalization, 41% patients (14 of 34) had an obstructive ventilatory defect, and one patient with scoliosis had mixed ventilatory dysfunction. Persistent abnormalities on brain MRI were 0% (0 of 7), 9% (2 of 23) and 57% (4 of 7) in CNS, ANS and cardiorespiratory failure group separately. Patients with HFMD may have abnormalities in neurological, motor, language, cognition, adaptive behaviour and respiratory function. Long-term follow-up programmes for children's neurodevelopmental and respiratory function may be warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/22221751.2021.1901612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009121PMC
December 2021

Nerve recognition in percutaneous transforaminal endoscopic discectomy using convolutional neural network.

Med Phys 2021 Mar 8. Epub 2021 Mar 8.

Biomedical Information Engineering Lab, The University of Aizu, Aizu-Wakamatsu City, Fukushima, 965-8580, Japan.

Purpose: Percutaneous transforaminal endoscopic discectomy (PTED) is one of the most common minimally invasive surgery methods used in clinic in recent years. In this study, we developed a computer-aided detection system (CADS) based on convolutional neural network (CNN) to automatically recognize nerve and dura mater images under PTED surgery.

Methods: We collected surgical videos from 65 patients with lumbar disc herniation who underwent PTED; we then converted the videos into images, and randomly divided some images into a training dataset, a validation dataset, test dataset. The training dataset and validation dataset were composed of 10 454 images containing nerve and dura mater from 50 randomly selected patients; test dataset contained 12 000 images from the remaining 15 patients.

Results: The results showed that sensitivity, specificity, and accuracy reached 90.90%, 93.68%, and 92.29%, respectively. CADS could recognize the nerve and dura mater with no significant difference (P > 0.05) between each patient in test dataset. In comparison with clinicians of different levels, the performance of CADS was lower than that of a spinal endoscopist, but significantly higher than that of general surgeons. With the assistance of CADS, the performance of the general surgeons approached that of the spinal endoscopist.

Conclusions: CNN can recognize well nerve and dura mater images in PTED surgery, and can help general surgeons to improve their ability to recognize tissues during the operation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mp.14822DOI Listing
March 2021

Multicolor and Multistage Response Electrochromic Color-Memory Wearable Smart Textile and Flexible Display.

ACS Appl Mater Interfaces 2021 Mar 3;13(10):12313-12321. Epub 2021 Mar 3.

Key Laboratory of Science & Technology of Eco-Textile, Jiangnan University, Ministry of Education, Wuxi, Jiangsu 214122, China.

Electrochromic materials have great application in soft displays and devices, but the application of ideal electrochromic textiles still faces challenges owing to the inconvenience of a continuous power supply. Here, electrochromic color-memory microcapsules (ECM-Ms-red, -yellow, and -blue) with a low drive voltage (2.0 V), coloration efficiency (921.6 cm C), a practical response rate (34.4 s), multistage response discoloration, and good color-memory performance (>72 h) and reversibility (≥1000 cycles) are developed. The color-memory performance is controlled by the energy difference of oxidation-reduction reactions. A multicolor and multistage response electrochromic color-memory wearable smart textile and flexible display are developed that are convenient and energy-efficient for application. The design philosophy of color-memory based on controllable energy difference of reactions has great potential application in sensors and smart textiles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c01333DOI Listing
March 2021

Herbicide selection promotes antibiotic resistance in soil microbiomes.

Mol Biol Evol 2021 Feb 16. Epub 2021 Feb 16.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.

Herbicides are one of the most widely used chemicals in agriculture. While they are known to be harmful to non-target organisms, the effects of herbicides on the composition and functioning of soil microbial communities remain unclear. Here we show that application of three widely used herbicides-glyphosate, glufosinate and dicamba-increase the prevalence of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in soil microbiomes without clear changes in the abundance, diversity and composition of bacterial communities. Mechanistically, these results could be explained by a positive selection for more tolerant genotypes that acquired several mutations in previously well-characterized herbicide and antibiotic resistance genes. Moreover, herbicide exposure increased cell membrane permeability and conjugation frequency of multidrug resistance plasmids, promoting ARG movement between bacteria. A similar pattern was found in agricultural soils across eleven provinces in China, where herbicide application, and the levels of glyphosate residues in soils, were associated with increased ARG and MGE abundances relative to herbicide-free control sites. Together, our results show that herbicide application can enrich ARGs and MGEs by changing the genetic composition of soil microbiomes, potentially contributing to the global antimicrobial resistance problem in agricultural environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/molbev/msab029DOI Listing
February 2021

P-C Bond Cleavage Induced Ni(II) Complexes Bearing Rare-Earth-Metal-Based Metalloligand and Reactivities toward Isonitrile, Nitrile, and Epoxide.

Inorg Chem 2021 Mar 14;60(5):3249-3258. Epub 2021 Feb 14.

Key Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, Anhui 241002, PR China.

Protonolysis of β-diketiminato (nacnac) rare-earth metal bis-alkyl complexes LLnR(THF) (Ln = Y and Lu) with 2 equiv of PhPNHPh or PhPCHNHPh afforded the bis-amido complexes LY(PhPNPh) and LLn(PhPCHNPh) (Ln = Y and Lu). Metalation of the latter complexes with 1 equiv of Ni(COD) led to the isolation of unusual heterobimetallic Ni(II)-Ln(III) complexes formed via P-C bond cleavage of one [PhPCHNPh] ligand. Notably, both the imine PhN═CH and phosphide PhP fragments from the P-C bond cleavage were trapped in the Ni(II)-Ln(III) core with a relatively weak interaction between the two metal centers. The Ni(II)-Y(III) complex have exhibited versatile reactivity, such as coordination of isonitrile to the Ni(II) center, insertion of nitrile with the coordinated imine, and ring-opening of the epoxide by nucleophilic attack from the phosphide group.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.0c03675DOI Listing
March 2021

Improving Pulmonary Infection Diagnosis with Metagenomic Next Generation Sequencing.

Front Cell Infect Microbiol 2020 26;10:567615. Epub 2021 Jan 26.

Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.

Pulmonary infections are among the most common and important infectious diseases due to their high morbidity and mortality, especially in older and immunocompromised individuals. However, due to the limitations in sensitivity and the long turn-around time (TAT) of conventional diagnostic methods, pathogen detection and identification methods for pulmonary infection with greater diagnostic efficiency are urgently needed. In recent years, unbiased metagenomic next generation sequencing (mNGS) has been widely used to detect different types of infectious pathogens, and is especially useful for the detection of rare and newly emergent pathogens, showing better diagnostic performance than traditional methods. There has been limited research exploring the application of mNGS for the diagnosis of pulmonary infections. In this study we evaluated the diagnostic efficiency and clinical impact of mNGS on pulmonary infections. A total of 100 respiratory samples were collected from patients diagnosed with pulmonary infection in Shanghai, China. Conventional methods, including culture and standard polymerase chain reaction (PCR) panel analysis for respiratory tract viruses, and mNGS were used for the pathogen detection in respiratory samples. The difference in the diagnostic yield between conventional methods and mNGS demonstrated that mNGS had higher sensitivity than traditional culture for the detection of pathogenic bacteria and fungi (95% vs 54%; p<0.001). Although mNGS had lower sensitivity than PCR for diagnosing viral infections, it identified 14 viral species that were not detected using conventional methods, including multiple subtypes of human herpesvirus. mNGS detected viruses with a genome coverage >95% and a sequencing depth >100× and provided reliable phylogenetic and epidemiological information. mNGS offered extra benefits, including a shorter TAT. As a complementary approach to conventional methods, mNGS could help improving the identification of respiratory infection agents. We recommend the timely use of mNGS when infection of mixed or rare pathogens is suspected, especially in immunocompromised individuals and or individuals with severe conditions that require urgent treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2020.567615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874146PMC
January 2021

Long-term androgen excess induces insulin resistance and non-alcoholic fatty liver disease in PCOS-like rats.

J Steroid Biochem Mol Biol 2021 Apr 26;208:105829. Epub 2021 Jan 26.

Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, 200032, Shanghai, China. Electronic address:

Objective: Women with polycystic ovary syndrome (PCOS) are at higher risk for metabolic disorders compared to healthy women, and about 51 % of women with PCOS suffer from non-alcoholic fatty liver disease (NAFLD). Investigation into the pathological mechanism behind this association will provide insights for the prevention and treatment of this complication.

Methods: Dihydrotestosterone (DHT), a nonaromatic androgen, was used to mimic the pathological conditions of hyperandrogenism and insulin resistance. Hematoxylin and eosin staining, Oil Red O staining, immunofluorescent staining, Western blots, and qRT-PCR were used to verify the hepatic steatosis and inflammation, and the latter two methods were also used for energy and mitochondrion-related assays. ELISA was used to measure the level of reactive oxygen species.

Results: Twelve weeks of DHT exposure led to obesity and insulin resistance as well as hepatic steatosis, lipid deposition, and different degrees of inflammation. The expression of molecules involved in respiratory chain and aerobic respiration processes, such as electron transfer complex II, pyruvate dehydrogenase, and succinate dehydrogenase complex subunit A, was inhibited. In addition, molecules associated with apoptosis and autophagy were also abnormally expressed, such as increased Bak mRNA, an increased activated caspase-3 to caspase-3 ratio, and increased Atg12 protein expression. All of these changes are associated with the mitochondria and lead to lipid deposition and inflammation in the liver.

Conclusions: Long-term androgen excess contributes to insulin resistance and hepatic steatosis by affecting mitochondrial function and causing an imbalance in apoptosis and autophagy, thus suggesting the pathogenesis of NAFLD in women with PCOS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jsbmb.2021.105829DOI Listing
April 2021

Self-Symmetrical 3D Hierarchical α-Calcium Sulfate Hemihydrate Twin-Flowers Composed of Microplates as a Renewable Material for Water Separation from Water-in-Oil Emulsion.

Inorg Chem 2021 Feb 29;60(4):2188-2194. Epub 2021 Jan 29.

Anhui Liuguo Chemical Co. Ltd., Tonggang Road 8, Tongling 244021, PR China.

Novel three-dimensional hierarchical α-calcium sulfate hemihydrate twin-flowers with a self-symmetrical structure (3D α-HH HTFs) are synthesized successfully assisted by trisodium citrate (TSC). The morphology of α-HH is closely dependent on TSC, and with increasing TSC concentration from 0 to 15 mM, the morphology gradually evolves from a long column to rod, hexagonal plate, twin-flower-like, and eventually microgranule. 3D α-HH HTFs are formed heterogeneous nucleation coupled with Ostwald ripening. The 3D α-HH HTFs are further used as an immobilized water material to separate water from a surfactant-stabilized water-in-oil emulsion, and exhibit excellent separation performance with a separation efficiency of 99.31 wt % and immobilization efficiency of 93.03 wt %. Impressively, the separated solid after water separation can be regenerated into 3D α-HH HTFs, which retain the high separation performance of the original 3D α-HH HTFs. This work demonstrates that 3D α-HH HTFs are highly promising in purifying oil with undesired water contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.0c02815DOI Listing
February 2021

The spatial distribution characteristics of coseismic landslides triggered by the Ms7.0 Lushan earthquake and Ms7.0 Jiuzhaigou earthquake in southwest China.

Environ Sci Pollut Res Int 2021 Jan 6. Epub 2021 Jan 6.

State Key Laboratory of GeoHazard Prevention and GeoEnvironment Protection, Chengdu University of Technology, Chengdu, 610059, China.

In recent years, a series of earthquakes have occurred in Sichuan province, southwest China. On April 20, 2013, 1013 landslides were triggered in the Lushan earthquake within the research area of 1215.87 km. On August 8, 2017, 821 landslides were induced in the Jiuzhaigou earthquake within the study area of 541.61 km. The slope, aspect, elevation, distance to the river, distance to fault, stratum lithology, curvature, and PGA as evaluation factors were chosen to research the distribution characteristics of coseismic landslides. The relationship between influence factors and distribution of coseismic landslides were analyzed using landslide area density (LAD), defined as the ratio between the coseismic landslides area and the total area under the specific classification of eight factors, and landslide number density (LND), interpreted as the number of landslides per square kilometer affected by an earthquake. Both the LAD and LND curves show the common correlations between distribution characteristics of coseismic landslides and the classification of eight factors. The high density of coseismic landslides was found at locations where the valley evolves from U-shaped to V-shaped, along slopes with an inclination between 20 and 50°. Due to the effect of river cutting erosion and human engineering activities, massive coseismic landslides appeared within the scope of 200 m from the river system. Eight hundred sixty small shallow coseismic landslides were found less than 3 km from the seismogenic fault in both earthquakes. Both concave and convex terrain has the same effect of promoting coseismic landslides. The strata of the Carboniferous and Cretaceous are more prone to coseismic landslides occurrence. In the event of another earthquake of similar magnitude in southwest China in the future, the research results can quickly predict the distribution of coseismic landslides and provide a scientific basis for emergency rescue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-11826-5DOI Listing
January 2021

TLR4-Associated IRF-7 and NFκB Signaling Act as a Molecular Link Between Androgen and Metformin Activities and Cytokine Synthesis in the PCOS Endometrium.

J Clin Endocrinol Metab 2021 Mar;106(4):1022-1040

Department of Physiology/Endocrinology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Context: Low-grade chronic inflammation is commonly seen in polycystic ovary syndrome (PCOS) patients with elevated levels of inflammatory cytokines in the endometrium.

Objective: This work aimed to increase the limited understanding of the mechanisms underlying cytokine synthesis and increased endometrial inflammation in PCOS patients.

Methods: Endometrial biopsy samples were collected from non-PCOS (n = 17) and PCOS (n = 22) patients either during the proliferative phase of the menstrual cycle or with hyperplasia. Endometrial explants were prepared from PCOS patients and underwent pharmacological manipulation in vitro. The expression and localization of toll-like receptor 2 (TLR2)/4, key elements of innate immune signal transduction and nuclear factor κB (NFκB) signaling pathways, and multiple cytokines were comprehensively evaluated by Western blotting, immunohistochemistry, and immunofluorescence in endometrial tissues.

Results: We demonstrated the distribution of protein expression and localization associated with the significantly increased androgen receptor, TLR2, and TLR4-mediated activation of interferon regulatory factor-7 (IRF-7) and NFκB signaling, cytokine production, and endometrial inflammation in PCOS patients compared to non-PCOS patients with and without endometrial hyperplasia. In vitro experiments showed that 5-dihydrotestosterone (DHT) enhanced androgen receptor, TLR4, IRF-7, and p-NFκB p65 protein expression along with increased interferon α (IFNα) and IFNɣ abundance. The effects of DHT on IRF-7, p-NFκB p65, and IFN abundance were abolished by flutamide, an antiandrogen. Although 17β-estradiol (E2) decreased p-IRF-7 expression with little effect on TLR-mediated IRF7 and NFκB signaling or on cytokine protein levels, exposure to metformin alone or in combination with E2 suppressed interleukin-1 receptor-associated kinase 4 (IRAK4), p-IRF-7, IRF-7, IκB kinase α (IKKα), p-NFκB p65, IFNɣ, and tumor necrosis factor α protein expression.

Conclusion: Cytokine synthesis and increased endometrial inflammation in PCOS patients are coupled to androgen-induced TLR4/IRF-7/NFκB signaling, which is inhibited by metformin treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1210/clinem/dgaa951DOI Listing
March 2021

Entropy Generation Methodology for Defect Analysis of Electronic and Mechanical Components-A Review.

Entropy (Basel) 2020 Feb 23;22(2). Epub 2020 Feb 23.

School of Mechanical and Electrical Engineering, Guilin University of Electronic Technology, Guilin 541004, China.

Understanding the defect characterization of electronic and mechanical components is a crucial step in diagnosing component lifetime. Technologies for determining reliability, such as thermal modeling, cohesion modeling, statistical distribution, and entropy generation analysis, have been developed widely. Defect analysis based on the irreversibility entropy generation methodology is favorable for electronic and mechanical components because the second law of thermodynamics plays a unique role in the analysis of various damage assessment problems encountered in the engineering field. In recent years, numerical and theoretical studies involving entropy generation methodologies have been carried out to predict and diagnose the lifetime of electronic and mechanical components. This work aimed to review previous defect analysis studies that used entropy generation methodologies for electronic and mechanical components. The methodologies are classified into two categories, namely, damage analysis for electronic devices and defect diagnosis for mechanical components. Entropy generation formulations are also divided into two detailed derivations and are summarized and discussed by combining their applications. This work is expected to clarify the relationship among entropy generation methodologies, and benefit the research and development of reliable engineering components.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/e22020254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516701PMC
February 2020

Report of Alliance of International Science Organizations on Disaster Risk Reduction (ANSO-DRR) Conference 2020.

Int J Environ Res Public Health 2020 11 26;17(23). Epub 2020 Nov 26.

Graduate School of Media and Governance, Keio University, Tokyo 252-0882, Japan.

This article summarizes the proceedings of the four-session meeting (webinar) conducted by the Alliance of International Science Organizations on Disaster Risk Reduction (ANSO-DRR) on 18 May 2020. ANSO-DRR is an international, nonprofit and nongovernmental scientific alliance bringing together academies of science, research organizations and universities which share a strong interest in disaster risk reduction in the regions along the land-based and maritime routes of the Belt and Road Initiative. ANSO-DRR convenes an annual meeting to review its work progress and discuss its scientific programs. The first session was the opening statements and was followed by the introduction and updates on ANSO-DRR in the second session. The third session was the depiction of the big picture of ANSO, the umbrella organization of ANSO-DRR, led by the Assistant Executive Director of ANSO, while the fourth session was a presentation of perspectives on the strategic development of ANSO-DRR. One of ANSO-DRR's key strategies is to enhance disaster mitigation and response through multidisciplinary cooperation among disaster and healthcare sciences (i.e., health emergency and disaster risk management (Health-EDRM)). It aims to enhance DRR efforts by performing as an instrument in connecting people along the Belt and Road regions, focusing on DRR resource and database development, involving higher education institutions in DRR efforts and increasing disaster resilience in built infrastructures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph17238772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731225PMC
November 2020

Study on the Effect of Streets' Space Forms on Campus Microclimate in the Severe Cold Region of China-Case Study of a University Campus in Daqing City.

Int J Environ Res Public Health 2020 11 12;17(22). Epub 2020 Nov 12.

Key Laboratory of Cold Region Urban and Rural Human Settlement Environment Science and Technology, Ministry of Industry and Information Technology, School of Architecture, Harbin Institute of Technology, Harbin 150006, China.

In urban areas, local microclimate is influenced by architectural forms, which will in turn affect human comfort. Taking Daqing as an example, this article studies the microclimate of a university campus in the severe cold area in China. Based on the space features of the streets, we categorize the streets into three types: open type, semi-open type, and street-entry type. Through analysis, this article researches microclimates of the three kinds of streets, the influence of building heating on the surrounding thermal environment, the relationship between streets' morphology features and microclimate and human comfort (physiological equivalent temperature, PET). By study and analysis, we have the following findings: for open-type streets, the average globe temperatures of streets with different orientations can reach 1.3 °C in winter because of the influence of sidewalk trees. For semi-open-type streets, streets temperature is under the influence of the locating directions of buildings. The maximum air temperature difference among streets with different building arrangements reaches 2.1 °C in winter. For street-entry-type streets, the height-width ratios and orientations of streets are related to the continuity degree of the street interfaces. The building interface acts as a heating element and affect the surrounding thermal environment by heat convection and heat radiation. Analysis demonstrates that heat convection has a more obvious effect on rising surrounding temperature than heat radiation. Buildings with higher heat radiation witness higher globe temperature. For street-entry-type streets and semi-open-type streets, the SVF (sky view factor) and L/C (plane opening rate) of streets are negatively correlated with temperature and PET, but positively correlated with wind speed. If the SVF increases 0.1, the air temperature will reduce 0.1 °C, the wind speed will increase 0.19 m/s, and the PET will reduce 0.7 °C.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph17228389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7696297PMC
November 2020

Multinuclear 2-(Quinolin-2-yl)quinoxaline-Coordinated Iridium(III) Complexes Tethered by Carbazole Derivatives: Synthesis and Photophysics.

Inorg Chem 2020 Dec 10;59(23):17096-17108. Epub 2020 Nov 10.

Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58108-6050, United States.

Five mono/di/trinuclear iridium(III) complexes (-) bearing the carbazole-derivative-tethered 2-(quinolin-2-yl)quinoxaline (quqo) diimine (N^N) ligand were synthesized and characterized. The photophysical properties of these complexes and their corresponding diimine ligands were systematically studied via UV-vis absorption, emission, and transient absorption (TA) spectroscopy and simulated by time-dependent density functional theory. All complexes possessed strong well-resolved absorption bands at <400 nm that have predominant ligand-based π,π* transitions and broad structureless charge-transfer (CT) absorption bands at 400-700 nm. The energies or intensities of these CT bands varied pronouncedly when the number of tethered Ir(quqo)(piq) (piq refers to 1-phenylisoquinoline) units, π conjugation of the carbazole derivative linker, or attachment positions on the carbazole linker were altered. All complexes were emissive at room temperature, with - showing near-IR (NIR) MLCT (metal-to-ligand charge-transfer)/LLCT (ligand-to-ligand charge-transfer) emission at ∼710 nm and and exhibiting red or NIR ILCT (intraligand charge-transfer)/LMCT (ligand-to-metal charge-transfer) emission in CHCl. In CHCN, - displayed an additional emission band at ca. 590 nm (ILCT/LMCT/MLCT/π,π* in nature) in addition to the 710 nm band. The different natures of the emitting states of - versus those of and also gave rise to different spectral features in their triplet TA spectra. It appears that the parentage and characteristics of the lowest triplet excited states in these complexes are mainly impacted by the π systems of the bridging carbazole derivatives and essentially no interactions among the Ir(quqo)(piq) units. In addition, all of the diimine ligands tethered by the carbazole derivatives displayed a dramatic solvatochromic effect in their emission due to the predominant intramolecular charge-transfer nature of their emitting states. Aggregation-enhanced emission was also observed from the mixed CHCl/ethyl acetate or CHCl/hexane solutions of these ligands.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.0c02366DOI Listing
December 2020

Changes in hydrological behaviours triggered by earthquake disturbance in a mountainous watershed.

Sci Total Environ 2021 Mar 28;760:143349. Epub 2020 Oct 28.

Research Institute for Geo-hydrological Protection, Italian National Research Council, Padova 351127, Italy.

Landslides induced by strong earthquakes often destroy large amounts of landscape vegetation which can trigger significant changes in runoff potential and flood flow. Little is known about hydrological behaviours imposed by co-seismic landslides and their post-earthquake evolution. Therefore, we collected time-series datasets (2007-2018) of underlying surface conditions (USC) changes including landslide expansion and recovery in a watershed affected by the Wenchuan earthquake to further quantify how the large physical disturbance affected the flood hydrological behaviours. The hydrological model HEC-HMS was calibrated and validated to predict the historical hydrological behaviours based on 5 min time-series data in rainfalls and streamflow (2018-2019), showing a good model performance with a mean Nash-Sutcliffe efficiency of 0.76. It was found that, shortly after the earthquake, the sharp expansion with 11% of landslide areas elevated the magnitudes of runoff potential, peak discharge, and runoff volume by >10%, and the peak to time for the high-magnitude flood was advanced by 25 min compared to the pre-earthquake levels. The tipping point along the hydrological disturbance-recovery trajectory was detected within 2011 with higher flood peaks and volumes, and the periods of 2011-2013 (i.e. 3-5 years post-earthquake) were deemed to be a rapid recovery period, revealing an unstable hydrological function. These findings are significant for clearly understanding the magnitude and timing, as well as greater risks of post-earthquake catastrophic flooding in earthquake-stricken regions. Additionally, the post-earthquake accompanied rainstorm-induced geohazards, which limited the recovery of landscape vegetation, triggering an undulant but clear recovery process (1-7 years post-earthquake) of hydrological behaviours. These findings promoted our understanding of the spatiotemporal evolution of hydrological behaviours triggered by the earthquake, and further contribute to the development of adaptation and mitigation strategies for the unpredictable flash floods triggered by future abrupt natural hazards in earthquake-affected regions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.143349DOI Listing
March 2021

Molecular strategy for the direct detection and identification of human enteroviruses in clinical specimens associated with hand, foot and mouth disease.

PLoS One 2020 9;15(11):e0241614. Epub 2020 Nov 9.

School of Public Health, Fudan University, Key Laboratory of Public Health Safety, Ministry of Education, Shanghai, China.

Background: Diseases caused by human enteroviruses (EVs) are a major global public health problem. Thus, the effective diagnosis of all human EVs infections and the monitoring of epidemiological and ecological dynamic changes are urgently needed.

Methods: Based on two comprehensive virological surveillance systems of hand, foot and mouth disease (HFMD), real-time PCR and nested RT-PCR (RT-snPCR) methods based on the enteroviral VP1, VP4-VP2 and VP4 regions were designed to directly detect all human EVs serotypes in clinical specimens.

Results: The results showed that the proposed serotyping strategy exhibit very high diagnostic efficiency (Study 1: 99.9%; Study 2: 89.5%), and the variance between the study was due to inclusion of the specific Coxsackie virus A6 (CVA6) real-time RT-PCR and VP4 RT-snPCR in Study 1 but not Study 2. Furthermore, only throat swabs were collected and analyzed in Study 2, whereas in Study 1, if a specific EV serotype was not identified in the primary stool sample, other sample types (rectal swab and throat swab) were further tested where available. During the study period from 2013 to 2018, CVA6 became one of the main HFMD causative agents, whereas the level of enterovirus A71 (EV-A71) declined in 2017.

Conclusion: The findings of this study demonstrate the appropriate application of PCR methods and the combination of biological sample types that are useful for etiological studies and propose a molecular strategy for the direct detection of human EVs in clinical specimens associated with HFMD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241614PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7652283PMC
December 2020

Enterovirus genomic load and disease severity among children hospitalised with hand, foot and mouth disease.

EBioMedicine 2020 Dec 6;62:103078. Epub 2020 Nov 6.

School of Public Health, Fudan University, Key Laboratory of Public Health Safety, Ministry of Education, Shanghai, China. Electronic address:

Background: Examining associations between viral genomic loads of enteroviruses and clinical severity is important for promoting and improving development of antiviral drugs related to hand, foot and mouth disease (HFMD).

Methods: Throat swabs were collected from HFMD cases at acute phase of illness using a standardized technique in a prospective study. The viral genomic load was categorized into low, medium, and high groups using parameters of real-time reverse transcription-polymerase chain reaction. The clinical severities were assessed with four indicators, respectively.

Findings: We analysed 1109 HFMD cases, including 538 children with CV-A6, 231 with CV-A16, 156 with EV-A71, 78 with CV-A10, 59 with CV-A4, and 47 with CV-A2. EV-A71 genomic load categories were associated with risks of diagnoses of CNS complications (p = 0.016), requiring systemic corticosteroids or IVIG (p = 0.011), intensive care unit admission (p = 0.002) and length of hospital stay over 5 days (p = 0.048). In the multivariate analyses, point estimates of adjusted odds ratio (OR) tended to increase with viral genomic loads for all four severe outcomes and ORs of highest viral genomic load were all significantly larger than one for EV-A71.

Interpretation: HFMD clinical severities positively associate with viral genomic loads of EV-A71 in throat swabs. Specific antiviral drugs should be developed to reduce enterovirus load and to alleviate the clinical severities for HFMD cases.

Funding: National Science Fund for Distinguished Young Scholars.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ebiom.2020.103078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653080PMC
December 2020

New Insight into the Mechanism of Multivalent Ion Hybrid Supercapacitor: From the Effect of Potential Window Viewpoint.

Small 2020 Nov 26;16(46):e2003403. Epub 2020 Oct 26.

Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education, School of Physical Science and Technology, Lanzhou University, Lanzhou, 730000, P. R. China.

Multivalent ion hybrid supercapacitors have been developed as the novel electrochemical energy storage systems due to their combined merits of high energy density and high power density. Nevertheless, there are still some challenges due to the limited understanding of the electrochemical behaviors of multivalent ions in the electrode materials, which greatly hinders the large scale applications of its based hybrid supercapacitors. Herein, the long-term electrochemical behaviors of MnO -based electrode in the divalent Mg ions electrolyte are systematically studied and linked with the morphological and electronic evolution of MnO by cycling at different potential windows (spanning to 1.2 V). It reveals that the different potential windows result in the different electrochemical behaviors, which can be divided into two ranges (below and above -0.2 V). And, the electrode cycled at a potential window of 0-1.2 V delivers the highest capacitance of 967 F g at a scan rate of 10 mV s , in which the MnO is transformed into a uniformly distributed and nonagglomerated nanoflake morphology promoting the intercalation and deintercalation of Mg ions. This study will enrich the understanding of the charge storage mechanism of multivalent ions and provide significant guidance on the performance improvement of the hybrid supercapacitors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202003403DOI Listing
November 2020

One-Dimensional -d Conjugated Coordination Polymer for Electrochromic Energy Storage Device with Exceptionally High Performance.

Adv Sci (Weinh) 2020 Oct 15;7(20):1903109. Epub 2020 Sep 15.

School of Materials Science and Engineering Nanyang Technological University Singapore 639798 Singapore.

The rational design of previously unidentified materials that could realize excellent electrochemical-controlled optical and charge storage properties simultaneously, are especially desirable and useful for fabricating smart multifunctional devices. Here, a facile synthesis of a 1D -d conjugated coordination polymer (Ni-BTA) is reported, consisting of metal (Ni)-containing nodes and organic linkers (1,2,4,5-benzenetetramine), which could be easily grown on various substrates via a scalable chemical bath deposition method. The resulting Ni-BTA film exhibits superior performances for both electrochromic and energy storage functions, such as large optical modulation (61.3%), high coloration efficiency (223.6 cm C), and high gravimetric capacity (168.1 mAh g). In particular, the Ni-BTA film can maintain its electrochemical recharge-ability and electrochromic properties even after 10 000 electrochemical cycles demonstrating excellent durability. Moreover, a smart energy storage indicator is demonstrated in which the energy storage states can be visually recognized in real time. The excellent electrochromic and charge storage performances of Ni-BTA films present a great promise for Ni-BTA nanowires to be used as practical electrode materials in various applications such as electrochromic devices, energy storage cells, and multifunctional smart windows.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.201903109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578889PMC
October 2020

Linking microbial community structure with molecular composition of dissolved organic matter during an industrial-scale composting.

J Hazard Mater 2021 Mar 15;405:124281. Epub 2020 Oct 15.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangdong Academy of Sciences, Guangzhou 510650, China.

This study explored the interactions between dissolved organic matter (DOM) composition and microbial community structure during an industrial-scale composting by Fourier transform ion cyclotron resonance mass spectrometry and 16S rRNA sequencing analysis. The results revealed that DOM from matured compost contained primarily lignins/carboxylic-rich alicyclic molecules (73.6%), the higher double bond equivalent (5.97) and aromaticity index (0.18), indicating that the molecular composition of DOM had changed substantially. Drastic changes in microbial community structure were also observed along with the DOM transformation process of composting. Network analysis further indicated that Caldicoprobacter, Bacillus, and Dechloromonas were associated with the most DOM subcategories. Caldicoprobacter could degrade carbohydrates, Bacillus accelerated the humification by transforming N-containing compounds, and Dechloromonas could degrade polycyclic aromatic hydrocarbons distributed in low O/C. These findings are helpful for understanding the molecular mechanisms of DOM transformation and humification of sludge composting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124281DOI Listing
March 2021

Epidemiological and clinical characteristics of 161 discharged cases with coronavirus disease 2019 in Shanghai, China.

BMC Infect Dis 2020 Oct 20;20(1):780. Epub 2020 Oct 20.

Shanghai Municipal Center for Disease Control and Prevention, No. 1380, West Zhongshan Road, Shanghai, 200336, China.

Background: In December 2019, the outbreak of coronavirus disease 2019 (COVID-19) began in Wuhan, China, and rapidly spread to other regions. We aimed to further describe the epidemiological and clinical characteristics of discharged COVID-19 cases and evaluate the public health interventions.

Methods: We collected epidemiological and clinical data of all discharged COVID-19 cases as of 17 February 2020 in Shanghai. The key epidemiological distributions were estimated and outcomes were also compared between patients whose illness were before 24 January and those whose illness were after 24 January.

Results: Of 161 discharged COVID-19 cases, the median age was 45 years, and 80 (49.7%) cases were male. All of the cases were categorized as clinical moderate type. The most common initial symptoms were fever (85.7%), cough (41.0%), fatigue (19.3%), muscle ache (17.4%), sputum production (14.9%), and there were six asymptomatic cases. 39 (24.2%) cases got infected in Shanghai, and three of them were second-generation cases of Shanghai native cases. The estimated median of the time from onset to first medical visit, admission, disease confirmation, and discharge for 161 cases was 1.0 day (95% CI, 0.6-1.2), 2.0 days (95% CI, 1.5-2.6), 5.2 days (95% CI, 4.6-5.7), 18.1 days (95% CI, 17.4-18.8), respectively. The estimated median of the time from admission to discharge was 14.0 days (95% CI, 13.3-14.6). The time from onset to first medical visit, admission and disease confirmation were all shortened after the Shanghai's first-level public health emergency response. In Cox regression model, the significant independent covariates for the duration of hospitalization were age, the time from onset to admission and the first-level public health emergency response.

Conclusions: Local transmission had occurred in Shanghai in late January 2020. The estimated median of the time from onset to discharge of moderate COVID-19 was 18.1 days in Shanghai. Time intervals from onset to first medical visit, admission and disease confirmation were all shortened after the Shanghai's first-level public health emergency response. Age, the first-level public health emergency response and the time from onset to admission were the impact factors for the duration of hospitalization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12879-020-05493-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573864PMC
October 2020

Two new species of the genus Xiphidiopsis Redtenbacher, 1891 (Tettigoniidae: Meconematinae: Meconematini) from Yunnan, China.

Zootaxa 2020 Sep 1;4845(1):zootaxa.4845.1.10. Epub 2020 Sep 1.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, P. R. China.

The paper reports two new species of the genus Xiphidiopsis Redtenbacher, 1891 from Yunnan, China, i.e. Xiphidiopsis (Dinoxiphidiopsis) bifurcatis sp. nov. and Xiphidiopsis (Xiphidiopsis) spoona sp. nov., and provides characteristic photographs of external morphology. The type specimens are deposited in the Museum of Hebei University, Baoding, P. R. China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11646/zootaxa.4845.1.10DOI Listing
September 2020

Notes on the genus Macroteratura Gorochov, 1993 (Tettigoniidae: Meconematinae: Meconematini) with description of one new species from China.

Zootaxa 2020 Sep 29;4858(1):zootaxa.4858.1.6. Epub 2020 Sep 29.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, P. R. China.

The paper deals with 7 species of the genus Macroteratura from China and provides information on examined specimens, of which one new species Macroteratura (Macroteratura) inospina Chen, Cui Chang sp. nov. is described and illustrated with characteristic photographs of four known species from China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11646/zootaxa.4858.1.6DOI Listing
September 2020

ECG-based Identity Validation during Bathing in Different Water Temperature.

Annu Int Conf IEEE Eng Med Biol Soc 2020 07;2020:5276-5279

This paper proposes a novel identity validation method using ECG signal measured during bathing at 5 different bathtub water temperature ranges, that are 37 ±0.5 °C, 38±0.5 °C, 39±0.5 °C, 40±0.5 °C and 41±0.5 °C, respectively. The experiment includes 5 male and 5 female subjects, each subject collects 2 ECG recordings at each bathtub water temperature range, one day one recording, 10 ECG recordings are collected from each subject, each ECG recording is 18 minutes long, the sampling rate is 200 Hz. During the data processing stage, we perform spectrum analysis, baseline wandering removal, 50 Hz electromagnetic interference removal, signal smoothing, R peaks detection, and QRS complex segmentation. During the classification stage, we perform identity validation using long short-term memory (LSTM) classification network. 5 classification models are trained based on different bathtub water temperature ranges and the cross-validation method is used. Preliminary validation results show that different bathtub water temperature has an important impact on the identity validation. In order to precisely and quickly perform identity validation at different bathtub water temperature ranges, the final classification model is trained based on the samples from 5 different bathtub water temperature ranges. The highest and average identity validation accuracies are 98.43% and 97.68%, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/EMBC44109.2020.9176107DOI Listing
July 2020

Growth arrest or drug target inactivity is not sufficient for persister formation in E. coli.

Discov Med 2020 Mar-Apr;29(157):103-112

Department of Infectious Diseases, Huashan Hospital of Fudan University, Shanghai 200040, China.

Persisters are a subpopulation of slow-growing or nondividing cells that are tolerant to antibiotics and are thought to be involved in persistent infections. The development of antibiotic tolerant phenotype is thought to be due to antibiotic target inactivity and is closely associated with growth arrest. While growth arrest and antibiotic target inactivity are widely believed to be important for persister formation, there have been inconsistent results and it has been difficult to determine whether growth arrest or antibiotic target inactivity is necessary or sufficient for persister formation. To address these questions, we used a novel approach to create antibiotic target inactivation via promoter swap to knock down quinolone drug target DNA gyrase subunit A (GyrA), as well as growth arrest via CRISPR interference to block key cell division protein (FtsZ) and a key ribosomal protein L28 (RpmB). Growth dynamics, relative target gene expression, cellular ATP levels and persister formation in the GyrA, FtsZ, and RpmB knockdown strains were compared with the control growing bacteria. Surprisingly, we found that the strains that had growth arrest induced by FtsZ or RpmB knockdown did not induce persister formation. Similarly, knockdown of GyrA, a quinolone drug target, did not induce persister cells tolerant to levofloxacin. In addition, ATP levels, a measure of cellular metabolism, were not reduced but increased in the GyrA, FtsZ, and RpmB knockdown strains compared with the control strain. Thus, we conclude that growth arrest or target inactivation is not sufficient to produce persister phenotype as commonly assumed and that cellular ATP levels did not correlate with persister formation. Further studies are needed to better understand how persisters are formed for improved treatment of persistent infections.
View Article and Find Full Text PDF

Download full-text PDF

Source
October 2020

LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis.

Cancer Cell Int 2020 22;20:460. Epub 2020 Sep 22.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, 130033 Jilin China.

Background: LncRNAs act as functional regulators in tumor progression through interacting with various signaling pathways in multiple types of cancer. However, the effect of LINC02418 on colorectal cancer (CRC) progression and the underling mechanisms remain unclear.

Methods: LncRNA expression profile in CRC tissues was investigated by the TCGA database. The expressional level of LINC02418 in CRC patients was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Kaplan-Meier analyses was used to investigate the correlation between LINC02418 and overall survival (OS) of CRC patients. Cell proliferative, migratory and invasive abilities were detected by CCK-8 assays, colony formation assays and trans-well assays in HCT116 and LoVo cells which were stably transduced with sh-LINC02418 or sh-NC. The binding between LINC02418 and miR-34b-5p, and the interaction between miR-34b-5p and BCL2 were determined by dual-luciferase assays. Western blot experiments were conducted to further explore the effect of miR-34b-5p on BCL2 signaling pathway. Rescue experiments were performed to uncover the role of LINC02418/miR-34b-5p/BCL2 axis in CRC progression.

Results: LINC02418 was upregulated in human colon cancer samples when compared with adjacent tissue, and its high expressional level correlated with poor prognosis of CRC patients. LINC02418 promoted cancer progression by enhancing tumor growth, cell mobility and invasiveness of colon cancer cells. Additionally, LINC02418 could physically bind to miR-34b-5p and subsequently affect BCL2 signaling pathway. Down-regulation of LINC02418 reduced cell proliferation, while transfection of miR-34b-5p inhibitor or BCL2 into LINC02418-silenced CRC cells significantly promoted CRC cells growth.

Conclusions: LINC02418 was upregulated in human CRC samples and could be used as the indicator for prediction of prognosis. LINC02418 acted as a tumor driver by negatively regulating cell apoptosis through LINC02418/miR-34b-5p/BCL2 axis in CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-020-01530-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507712PMC
September 2020

Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots.

BMC Plant Biol 2020 Sep 21;20(1):435. Epub 2020 Sep 21.

School of Agriculture and Food Science, Zhejiang Agriculture & Forestry University, Hangzhou, 311300, China.

Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage.

Results: For roots stored under 4 °C condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13 °C). The activities of SOD, CAT, APX, O producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway, especially phenylpropanoids and followed by starch and sucrose metabolism. L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of roots. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

Conclusions: Our proteomic and metabolic profile analysis of sweetpotatoes stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (CAT/APX activity) data may be explained by higher HO levels and increased ascorbate redox states, which enhanced the CAT/APX activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-020-02642-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507648PMC
September 2020