Publications by authors named "Peng Cheng"

1,613 Publications

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Fabrication of Moisture-Responsive Crystalline Smart Materials for Water Harvesting and Electricity Transduction.

J Am Chem Soc 2021 May 14. Epub 2021 May 14.

State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin, 300071, People's Republic of China.

It is of profound significance with regard to the global energy crisis to develop new techniques and materials that can convert the chemical potential of water into other forms of energy, especially electricity. To address this challenge, we built a new type of energy transduction pathway (humidity gradients → mechanical work → electrical power) using moisture-responsive crystalline materials as the media for energy transduction. Single-crystal data revealed that a flexible zeolitic pyrimidine framework material, , could undergo a reversible structural transformation (β to α phase) with a large unit cell change upon moisture stimulus. Dynamic water vapor sorption analysis showed a gate-opening effect with a steep uptake at as low as 10% relative humidity (RH). The scalable green synthesis approach and the fast water vapor adsorption-desorption kinetics made an excellent sorbent to harvest water from arid air, as verified by real water-harvesting experiments. Furthermore, we created a gradient distribution strategy to fabricate polymer-hybridized mechanical actuators based on that could perform reversible bending deformation upon a variation of the humidity gradient. This mechanical actuator showed remarkable durability and reusability. Finally, coupling the moisture-responsive actuator with a piezoelectric transducer further converted the mechanical work into electrical power. This work offers a new type of moisture-responsive smart material for energy transduction and provides an in-depth understanding of the responsive mechanism at the molecular level.
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http://dx.doi.org/10.1021/jacs.1c01831DOI Listing
May 2021

Differential Effects of Trichostatin A on Mouse Embryogenesis and Development.

Reproduction 2021 May 1. Epub 2021 May 1.

Q Zhou, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, Jilin University First Hospital, Changchun, China.

Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, can significantly improve the reprogramming efficiency of somatic cells. However, whether TSA has detrimental effect on other kinds of embryos is largely unknown because of the lack of integrated analysis of TSA effect on natural fertilized embryos. To investigate the effect of TSA on mouse embryo development, we analyzed preimplantation and post-implantation development of in vivo, in vitro fertilized, and parthenogenetic embryos treated with TSA at different concentrations and durations. In vivo fertilized embryos appeared to be the most sensitive to TSA treatment among the three groups, and the blastocyst formation rate decreased sharply as TSA concentration and treatment time increased. TSA treatment also reduced the livebirth rate for in vivo fertilized embryos from 56.59% to 38.33%, but did not significantly affect postnatal biological functions such as the pups' reproductive performance and their ability for spatial learning and memory. Further analysis indicated that the acetylation level of H3K9 and H4K5 was enhanced by TSA treatment at low concentrations, while DNA methylation appeared to be also disturbed by TSA treatment only at high concentration. Thus, our data indicates that TSA has different effects on preimplantation embryonic development depending on the nature of the embryo's reproductive origin, the TSA concentration and treatment time, whereas the effect of TSA at indicated concentration on postnatal function was minor.
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http://dx.doi.org/10.1530/REP-21-0020DOI Listing
May 2021

Personalized radioiodine therapy for thyroid cancer patients with known disease.

Fac Rev 2021 7;10:36. Epub 2021 Apr 7.

Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.

Radioactive iodine (RAI) I is a targeted therapy for patients with RAI-avid follicular cell-derived thyroid cancer. However, the responsiveness to I therapy varies among thyroid cancer patients mainly owing to differential RAI uptake and RAI radiosensitivity among patients' lesions. A personalized approach to maximize I therapeutic efficacy is proposed based on recent scientific advances and future opportunities.
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http://dx.doi.org/10.12703/r/10-36DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103907PMC
April 2021

CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone.

ACS Sens 2021 May 11. Epub 2021 May 11.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Quantitative polymerase chain reaction (qPCR) is widely applied in foodborne pathogen detection and diagnosis. According to the cycles of threshold (Ct) values of qPCR testing, samples are judged as positive or negative. However, samples with Ct values in the gray zone are classified as "possibly positive" and required to be tested again. Repetitive qPCR may not eliminate the uncertain results but increase the workload of detection. CRISPR/Cas12a can specifically recognize the nucleic acid of the nM level and then indiscriminately slash the single-strand DNA with multiple turnovers. In this way, the detection signals can be greatly amplified. Here, we propose a CRISPR-based checking method to solve gray zone problems. After qPCR testing, the screening gray zone samples can be successfully checked by the CRISPR/Cas12a method. Furthermore, to conduct CRISPR reaction assay more conveniently and prevent possible aerosol contamination in the operational process, a gray zone checking cassette is designed. African swine fever virus (ASFV) is selected as an example to demonstrate the feasibility of the CRISPR-based checking method. Of 28 real swine blood samples, 6 ASFV qPCR gray zone samples are successfully checked. The CRISPR-based checking method provides a novel solution to eliminate gray zone sample problems with no additional effects on the PCR, which is operable and applicable in practical detection. The entire process can be completed within 10-15 min. This method will be a good supplementary and assistance for qPCR-based detection, especially in the diagnosis of diseases such as COVID-19.
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http://dx.doi.org/10.1021/acssensors.1c00515DOI Listing
May 2021

Differences in the intestinal microbiota between insecticide-resistant and -sensitive Aedes albopictus based on full-length 16S rRNA sequencing.

Microbiologyopen 2021 Mar;10(2):e1177

Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining, China.

The intestinal symbiotic bacteria of Aedes albopictus play a potential role in host resistance to insecticides. In this study, we sequenced the full-length of 16S rRNA and analyzed the differences in the intestinal microbiota between deltamethrin-resistant and -sensitive Ae. albopictus. Symbiotic bacteria were cultured and analyzed using six types of culture media in aerobic and anaerobic environments. We found significant differences in the diversity and abundance of the intestinal microbiota of the two strains of Ae. albopictus. The symbiotic bacteria cultured in vitro were found to be mainly facultative anaerobes. The cultured bacteria such as Serratia oryzae and Acinetobacter junii may function to promote the development of insecticide resistance. This work indicates that intestinal bacteria may contribute to the enhancement of insecticide resistance of Ae. albopictus It also highlights the analytical advantage of full-length 16S rRNA sequencing to study the intestinal microbiota of mosquitoes.
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http://dx.doi.org/10.1002/mbo3.1177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087943PMC
March 2021

D3DistalMutation: a Database to Explore the Effect of Distal Mutations on Enzyme Activity.

J Chem Inf Model 2021 May 2. Epub 2021 May 2.

CAS Key Laboratory of Receptor Research; Drug Discovery and Design Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Enzyme activity is affected by amino acid mutations, particularly mutations near the active site. Increasing evidence has shown that distal mutations more than 10 Å away from the active site may significantly affect enzyme activity. However, it is difficult to study the enzyme regulation mechanism of distal mutations due to the lack of a systematic collection of three-dimensional (3D) structures, highlighting distal mutation site and the corresponding enzyme activity change. Therefore, we constructed a distal mutation database, namely, D3DistalMutation, which relates the distal mutation to enzyme activity. As a result, we observed that approximately 80% of distal mutations could affect enzyme activity and 72.7% of distal mutations would decrease or abolish enzyme activity in D3DistalMutation. Only 6.6% of distal mutations in D3DistalMutation could increase enzyme activity, which have great potential to the industrial field. Among these mutations, the Y to F, S to D, and T to D mutations are most likely to increase enzyme activity, which sheds some light on industrial catalysis. Distal mutations decreasing enzyme activity in the allosteric pocket play an indispensable role in allosteric drug design. In addition, the pockets in the enzyme structures are provided to explore the enzyme regulation mechanism of distal mutations. D3DistalMutation is accessible free of charge at https://www.d3pharma.com/D3DistalMutation/index.php.
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http://dx.doi.org/10.1021/acs.jcim.1c00318DOI Listing
May 2021

Pharmacological Activities and Mechanisms of Hirudin and Its Derivatives - A Review.

Front Pharmacol 2021 16;12:660757. Epub 2021 Apr 16.

State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu, China.

Hirudin, an acidic polypeptide secreted by the salivary glands of (also known as "Shuizhi" in traditional Chinese medicine), is the strongest natural specific inhibitor of thrombin found so far. Hirudin has been demonstrated to possess potent anti-thrombotic effect in previous studies. Recently, increasing researches have focused on the anti-thrombotic activity of the derivatives of hirudin, mainly because these derivatives have stronger antithrombotic activity and lower bleeding risk. Additionally, various bioactivities of hirudin have been reported as well, including wound repair effect, anti-fibrosis effect, effect on diabetic complications, anti-tumor effect, anti-hyperuricemia effect, effect on cerebral hemorrhage, and others. Therefore, by collecting and summarizing publications from the recent two decades, the pharmacological activities, pharmacokinetics, novel preparations and derivatives, as well as toxicity of hirudin were systematically reviewed in this paper. In addition, the clinical application, the underlying mechanisms of pharmacological effects, the dose-effect relationship, and the development potential in new drug research of hirudin were discussed on the purpose of providing new ideas for application of hirudin in treating related diseases.
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http://dx.doi.org/10.3389/fphar.2021.660757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085555PMC
April 2021

A hybrid differential evolution based on gaining‑sharing knowledge algorithm and harris hawks optimization.

PLoS One 2021 30;16(4):e0250951. Epub 2021 Apr 30.

School of Aeronautics and Astronautics, Sichuan University, Chengdu, China.

Differential evolution (DE) is favored by scholars for its simplicity and efficiency, but its ability to balance exploration and exploitation needs to be enhanced. In this paper, a hybrid differential evolution with gaining-sharing knowledge algorithm (GSK) and harris hawks optimization (HHO) is proposed, abbreviated as DEGH. Its main contribution lies are as follows. First, a hybrid mutation operator is constructed in DEGH, in which the two-phase strategy of GSK, the classical mutation operator "rand/1" of DE and the soft besiege rule of HHO are used and improved, forming a double-insurance mechanism for the balance between exploration and exploitation. Second, a novel crossover probability self-adaption strategy is proposed to strengthen the internal relation among mutation, crossover and selection of DE. On this basis, the crossover probability and scaling factor jointly affect the evolution of each individual, thus making the proposed algorithm can better adapt to various optimization problems. In addition, DEGH is compared with eight state-of-the-art DE algorithms on 32 benchmark functions. Experimental results show that the proposed DEGH algorithm is significantly superior to the compared algorithms.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250951PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087089PMC
April 2021

The natural product lapiferin inhibits cell proliferation and promotes cell apoptosis in gingival squamous cell carcinoma via P21 regulation.

Mol Med Rep 2021 Jul 28;24(1). Epub 2021 Apr 28.

Department of Stomatology, School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, P.R. China.

Gingival squamous cell carcinoma (GSCC) is responsible fora large proportion of oral cavity malignancies. GSCC is characterized by rapid cell growth, and progressive invasion and migration. P21 is a widely recognized tumor suppressor, which is induced by P53 activation; however, drugs that aim to promote P21‑mediated tumor suppression remain to be identified. A natural compound library was used to perform broad‑spectrum screening of drugs that could promote P21 expression. Subsequently, the effects of the screened drug on GSCC cell proliferation and apoptosis were evaluated. The results of the present study suggested that lapiferin was the most effective natural compound that promoted the expression of P21 at both mRNA and protein levels. Lapiferin inhibited proliferation and enhanced apoptosis of YD‑38 GSCC cells in a dose‑dependent manner. Furthermore, following treatment with lapiferin, the critical cell cycle regulators cell division cycle 25C and cyclin B1 and tumor cell proliferation markers proliferating cell nuclear antigen and Ki67 were markedly decreased. In addition, pro‑apoptotic proteins were promoted following treatment of YD‑38 cells with lapiferin. Following the depletion ofP21 expression, lapiferin‑mediated inhibition of cell proliferation and enhancement of cell apoptosis were significantly reduced. These results indicated that lapiferin may exert potent antitumor effects on GSCC via regulation of P21; therefore, lapiferin may be considered a potential, natural therapeutic agent for the treatment of GSCC.
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http://dx.doi.org/10.3892/mmr.2021.12121DOI Listing
July 2021

Model-based evaluation of alternative reactive class closure strategies against COVID-19.

medRxiv 2021 Apr 23. Epub 2021 Apr 23.

There are contrasting results concerning the effect of reactive school closure on SARS-CoV-2 transmission. To shed light on this controversy, here we develop a data-driven computational model of SARS-CoV-2 transmission to investigate mechanistically the effect on COVID-19 outbreaks of school closure strategies based on syndromic surveillance and antigen screening of students. We found that by reactively closing classes based on syndromic surveillance, SARS-CoV-2 infections are reduced by no more than 13.1% (95%CI: 8.6%-20.2 %), due to the low probability of timely symptomatic case identification among the young population. We thus investigated an alternative triggering mechanism based on repeated screening of students using antigen tests. Should population-level social distancing measures unrelated to schools enable maintaining the reproduction number ( ) at 1.3 or lower, an antigen-based screening strategy is estimated to fully prevent COVID-19 outbreaks in the general population. Depending on the contribution of schools to transmission, this strategy can either prevent COVID-19 outbreaks for up to 1.9 or to at least greatly reduce outbreak size in very conservative scenarios about school contribution to transmission. Moving forward, the adoption of antigen-based screenings in schools could be instrumental to limit COVID-19 burden while vaccines continue to roll out through 2021, especially in light of possible continued emergence of SARS-CoV-2 variants.
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http://dx.doi.org/10.1101/2021.04.18.21255683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077629PMC
April 2021

Clinical therapeutic effects of combined methotrexate and other chemotherapeutic agents in treating children and young patients with osteosarcoma: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(17):e25564

Department of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.

Background: Osteosarcoma is one of the most common primary bone tumour in children and young patients, and the third most common among adults. Its main treatment option is currently based on neoadjuvant or adjuvant chemoradiotherapy along with the lesion's surgical resection. The current study's primary aim is to examine the clinical therapeutic impacts of combined methotrexate, along with other chemotherapeutic agents to treat children and young adults suffering from osteosarcoma.

Methods: We will perform a comprehensive literature search in English database (PubMed, EMBASE, Cochran Library CINAHL, and PsycINFO) and Chinese database (Chinese National Knowledge Infrastructure, VIP information database, Chinese Biomedical Database, and WanFang Database) with no language restriction from their inception to the search date. Additionally, two independent authors will screen the works of literature obtained from these databases, obtain information, and examine the risks of data included for the studies' bias. Furthermore, we intend to employ the Q statistics as well as I2 statistics to calculate heterogeneity among each study's analysis. Accordingly, we will utilize the funnel plots and Egger test to assess the possibility of publication bias where relevant.

Results: The current study aims to provide significant information regarding the clinical therapeutic impacts of combines methotrexate along with other chemotherapeutic agents to treat children and young adults suffering from osteosarcoma.

Conclusions: The present study will generate compelling evidence of combined methotrexate as well as other chemotherapeutic agents for osteosarcoma among children and young adults. Also, it will provide clinical practice suggestions.

Ethics And Dissemination: The study is founded upon published data. Therefore, there is no requirement for ethics approval.

Osf Registration Number: March 26, 2021.osf.io/a23rc. (https://osf.io/a23rc/).
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http://dx.doi.org/10.1097/MD.0000000000025564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084082PMC
April 2021

The impact of COVID-19 lockdown on atmospheric CO in Xi'an, China.

Environ Res 2021 Apr 22;197:111208. Epub 2021 Apr 22.

State Key Laboratory of Loess and Quaternary Geology, CAS Center for Excellence in Quaternary Science and Global Change, Institute of Earth Environment, Chinese Academy of Sciences, Xi'an, 710061, China; Shaanxi Provincial Key Laboratory of Accelerator Mass Spectrometry Technology and Application, Joint Xi'an AMS Center Between IEECAS and Xi'an Jiaotong University, Xi'an, 710061, China.

Lockdown measures to control the spread of the novel coronavirus disease (COVID-19) sharply limited energy consumption and carbon emissions. The lockdown effect on carbon emissions has been studied by many researchers using statistical approaches. However, the lockdown effect on atmospheric carbon dioxide (CO) on an urban scale remains unclear. Here we present CO concentration and carbon isotopic (δC) measurements to assess the impact of COVID-19 control measures on atmospheric CO in Xi'an, China. We find that CO concentrations during the lockdown period were 7.5% lower than during the normal period (prior to the Spring Festival, Jan 25 to Feb 4, 2020). The observed CO (total CO minus background CO) during the lockdown period was 52.3% lower than that during the normal period, and 35.7% lower than the estimated CO with the effect of weather removed. A Keeling plot shows that in contrast CO concentrations and δC were weakly correlated (R = 0.18) during the lockdown period, reflecting a change in CO sources imposed by the curtailment of traffic and industrial emissions. Our study also show that the sharp reduction in atmospheric CO during lockdown were short-lived, and returned to normal levels within months after lockdown measures were lifted.
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http://dx.doi.org/10.1016/j.envres.2021.111208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061636PMC
April 2021

Controlled Thermal Conversion Strategy to Provide Metal-Organic Framework-Supported Composite Catalysts.

Inorg Chem 2021 May 23;60(9):6514-6520. Epub 2021 Apr 23.

Department of Chemistry and Key Laboratory of Advanced Energy Material Chemistry, College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

Metal-organic framework (MOF)-supported metal/metal compound nanoparticles (NPs) have emerged as a new class of composite catalysts. However, huge challenges prevail in placing such NPs in the MOF pores because of the poor solubility of metal/metal oxides, limited availability of suitable precursors, metastable attribute of given metal ions, and lower thermal stability of MOFs compared to conventional porous materials. Based on the difference between the thermal stability of the precursor and MOFs, we successfully developed a controlled thermal conversion (CTC) method to load cobalt(II) oxide (CoO) NPs into the framework of MOF (MIL-101) to conveniently obtain a composite catalyst, CoO@MIL-101, which is a very rare example of pure CoO NP-loaded composite catalyst that shows excellent catalytic activity in the selective oxidation of benzyl alcohol. This CTC strategy opens up a pathway for impregnating MOF supports with specific NPs, which is further confirmed by preparing the first CuBr@MOF-type composite catalyst.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00322DOI Listing
May 2021

Enhanced Identification of Novel Potential Variants for Appendicular Lean Mass by Leveraging Pleiotropy With Bone Mineral Density.

Front Immunol 2021 6;12:643894. Epub 2021 Apr 6.

Department of Geriatrics, National Key Clinical Specialty, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.

Strong relationships have been found between appendicular lean mass (ALM) and bone mineral density (BMD). It may be due to a shared genetic basis, termed pleiotropy. By leveraging the pleiotropy with BMD, the aim of this study was to detect more potential genetic variants for ALM. Using the conditional false discovery rate (cFDR) methodology, a combined analysis of the summary statistics of two large independent genome wide association studies (GWAS) of ALM (n = 73,420) and BMD (n = 10,414) was conducted. Strong pleiotropic enrichment and 26 novel potential pleiotropic SNPs were found for ALM and BMD. We identified 156 SNPs for ALM (cFDR <0.05), of which 74 were replicates of previous GWASs and 82 were novel SNPs potentially-associated with ALM. Eleven genes annotated by 31 novel SNPs (13 pleiotropic and 18 ALM specific) were partially validated in a gene expression assay. Functional enrichment analysis indicated that genes corresponding to the novel potential SNPs were enriched in GO terms and/or KEGG pathways that played important roles in muscle development and/or BMD metabolism (adjP <0.05). In protein-protein interaction analysis, rich interactions were demonstrated among the proteins produced by the corresponding genes. In conclusion, the present study, as in other recent studies we have conducted, demonstrated superior efficiency and reliability of the cFDR methodology for enhanced detection of trait-associated genetic variants. Our findings shed novel insight into the genetic variability of ALM in addition to the shared genetic basis underlying ALM and BMD.
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http://dx.doi.org/10.3389/fimmu.2021.643894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056257PMC
April 2021

Fabrication of Fe3O4/ZIF-8 nanocomposite for simultaneous removal of copper and arsenic from water/soil/swine urine.

J Environ Manage 2021 Apr 17;290:112626. Epub 2021 Apr 17.

Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People's Republic of China; Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People's Republic of China. Electronic address:

In this study, a magnetic nanocomposite (denoted as FZ) was fabricated using FeOand zeolitic imidazolate framework-8 (ZIF-8), based on a coprecipitation method. FZ could efficiently remove Cu(Cu(II)) and AsO (As(III)) ions simultaneously from water, soil, and swine urine samples through hydrogen bonding and electrostatic interactions. The Cu(II) and As(III) removal efficiencies of the optimal FZ sample increased gradually with time and reached 99.1% and 98.4%, respectively, in 180 min. The maximum adsorption capacities of FZ4 for Cu(II) and As(III) were determined to be 33.48 mg/g and 21.12 mg/g, respectively. Additionally, the FZ with a high saturation magnetization (49.8 emu/g) was easily recovered from aqueous solutions and soil samples. Furthermore, zebrafish experiments indicated that FZ possessed a high biosafety. Thus, this study introduces a promising method for treating water, soil, and swine urine samples contaminated with Cu(II) and As(III), and verifies that FZ is suitable for practical application.
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http://dx.doi.org/10.1016/j.jenvman.2021.112626DOI Listing
April 2021

Polyacetylene glucosides from the florets of Carthamus tinctorius and their anti-inflammatory activity.

Phytochemistry 2021 Apr 16;187:112770. Epub 2021 Apr 16.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:

Five previously undescribed polyacetylene glucosides, namely, four C- and one C-acetylenes, together with three known analogues, were isolated from the florets of Carthamus tinctorius L. The structures of these novel compounds were elucidated to be (5R)-5-acetoxy-8,10,12-tetradecatriyne-1-O-β-D-glucopyranoside, (2Z)-decaene-4,6,8-triyne-1-O-β-D-glucopyranoside, (8Z)-1-[(3-O-β-D-glucosyl)-isovaleroyloxy]-8-decaene-4,6-diyne, (8Z)-decaene-1-isovaleroyloxy-4,6-diyne-10-O-β-D-glucopyranoside, and (2E,8E)-decadiene-4,6-diyne-1-O-β-D-glucopyranoside via spectroscopic and chemical methods. All of the isolated compounds were tested for cytotoxicity against cancer cell lines, antibacterial activity, and inhibitory effects on the lipopolysaccharide (LPS)-induced nitric oxide (NO) production. The results showed that (5R)-5-acetoxy-8,10,12-tetradecatriyne-1-O-β-D-glucopyranoside significantly inhibited LPS-induced NO production in RAW264.7 cells in a dose-dependent manner.
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http://dx.doi.org/10.1016/j.phytochem.2021.112770DOI Listing
April 2021

Differential Cell Line Susceptibility to Crimean-Congo Hemorrhagic Fever Virus.

Front Cell Infect Microbiol 2021 24;11:648077. Epub 2021 Mar 24.

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.

Crimean-Congo hemorrhagic fever (CCHF) is a severe tick-borne viral disease of global concerns due to the increasing incidence and lack of effective treatments. The causative agent, CCHF virus (CCHFV), has been characterized for years; however, its tropism in cell lines of different host and tissue origins remains unclear. This study characterized the susceptibility of 16 human and 6 animal cell lines to CCHFV. Increased viral load and viral nucleoprotein expression, and productive CCHFV replication were detected in human vascular (HUVEC), renal (SW-13 and HEK-293), hepatic (Huh7), and cerebral (U-87 MG) cell lines, which were considered CCHFV-highly permissive cell lines. Renal cell lines derived from monkey and dog could also support CCHFV replication. This study evaluated the susceptibility of different cell lines to CCHFV and identified CCHFV-permissive cell lines. Our findings raise concerns regarding the use of cell lines in studies of CCHFV and may have important implications for further fundamental research, which would promote understanding of CCHFV pathogenesis and transmission, as well as benefit designing strategies for disease prevention and control.
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http://dx.doi.org/10.3389/fcimb.2021.648077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044861PMC
March 2021

Review of Natural Resources With Vasodilation: Traditional Medicinal Plants, Natural Products, and Their Mechanism and Clinical Efficacy.

Front Pharmacol 2021 1;12:627458. Epub 2021 Apr 1.

State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

For decades, chronic diseases including cardiovascular and cerebrovascular diseases (CCVDs) have plagued the world. Meanwhile, we have noticed a close association between CCVDs and vascular lesions, such as hypertension. More focus has been placed on TMPs and natural products with vasodilation and hypotension. TMPs with vasodilatory and hypotensive activities are mainly from , , and (such as , , , , etc.) whereas natural products eliciting vasorelaxant potentials were primarily from flavonoids, phenolic acids and alkaloids (such as apigenin, puerarin, curcumin, sinomenine, etc.). Furthermore, the data analysis showed that the vasodilatory function of TMPs was mainly concerned with the activation of eNOS, while the natural products were primarily correlated with the blockage of calcium channel. Thus, TMPs will be used as alternative drugs and nutritional supplements, while natural products will be considered as potential therapies for CCVDs in the future. This study provides comprehensive and valuable references for the prevention and treatment of hypertension and CCVDs and sheds light on the further studies in this regard. However, since most studies are and preclinical, there is a need for more in-depth researches and clinical trials to understand the potential of these substances.
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http://dx.doi.org/10.3389/fphar.2021.627458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048554PMC
April 2021

Risk Compounds, Preclinical Toxicity Evaluation, and Potential Mechanisms of Chinese Materia Medica-Induced Cardiotoxicity.

Front Pharmacol 2021 30;12:578796. Epub 2021 Mar 30.

State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Chinese materia medica (CMM) has been applied for the prevention and treatment of diseases for thousands of years. However, arrhythmia, myocardial ischemia, heart failure, and other cardiac adverse reactions during CMM application were gradually reported. CMM-induced cardiotoxicity has aroused widespread attention. Our review aimed to summarize the risk compounds, preclinical toxicity evaluation, and potential mechanisms of CMM-induced cardiotoxicity. All relevant articles published on the PubMed, Embase, and China National Knowledge Infrastructure (CNKI) databases for the latest twenty years were searched and manually extracted. The risk substances of CMM-induced cardiotoxicity are relatively complex. A single CMM usually contains various risk compounds, and the same risk substance may exist in various CMM. The active and risk substances in CMM may be transformed into each other under different conditions, such as drug dosage, medication methods, and body status. Generally, the risk compounds of CMM-induced cardiotoxicity can be classified into alkaloids, terpenoids, steroids, heavy metals, organic acids, toxic proteins, and peptides. Traditional evaluation methods of chemical drug-induced cardiotoxicity primarily include cardiac function monitoring, endomyocardial biopsy, myocardial zymogram, and biomarker determination. In the preclinical stage, CMM-induced cardiotoxicity should be systematically evaluated at the overall, tissue, cellular, and molecular levels, including cardiac function, histopathology, cytology, myocardial zymogram, and biomarkers. Thanks to the development of systematic biology, the higher specificity and sensitivity of biomarkers, such as genes, proteins, and metabolic small molecules, are gradually applied for evaluating CMM-induced cardiotoxicity. Previous studies on the mechanisms of CMM-induced cardiotoxicity focused on a single drug, monomer or components of CMM. The interaction among ion homeostasis (sodium, potassium, and calcium ions), oxidative damage, mitochondrial injury, apoptosis and autophagy, and metabolic disturbance is involved in CMM-induced cardiotoxicity. Clarification on the risk compounds, preclinical toxicity evaluation, and potential mechanisms of CMM-induced cardiotoxicity must be beneficial to guide new CMM development and post-marketed CMM reevaluation.
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http://dx.doi.org/10.3389/fphar.2021.578796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044783PMC
March 2021

Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates.

Virol Sin 2021 Apr 9. Epub 2021 Apr 9.

Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

The ongoing coronavirus disease 2019 (COVID-19) pandemic caused more than 96 million infections and over 2 million deaths worldwide so far. However, there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the disease causative agent. Vaccine is the most effective approach to eradicate a pathogen. The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations. Here we evaluated the safety, immunogenicity, and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates. Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates, and subsequently provided partial (in low dose) or full (in high dose) protection of challenge in the tested animals. In addition, passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice. These results warranted positive outcomes in future clinical trials in humans.
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http://dx.doi.org/10.1007/s12250-021-00376-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034048PMC
April 2021

Manipulation of the Magnetic Anisotropy of Single Mn Atom via Molecular Ligands.

Nano Lett 2021 Apr 8;21(8):3566-3572. Epub 2021 Apr 8.

Department of Physics, Tsinghua University, Beijing 100084, China.

Magnetic anisotropy is essential for permanent magnets to maintain their magnetization along specific directions. Understanding and controlling the magnetic anisotropy on a single-molecule scale are challenging but of fundamental importance for the future's spintronic technology. Here, by using scanning tunneling microscopy (STM), we demonstrated the ability to control the magnetic anisotropy by tuning the ligand field at the single-molecule level. We constructed a molecular magnetic complex with a single Mn atom and an organic molecule (4,4'-biphenyldicarbonitrile) as a ligand via atomic manipulation. Inelastic tunneling spectra (IETS) show that the Mn complex has much larger axial magnetic anisotropy than individual Mn atoms, and the anisotropy energy can be tuned by the coupling strength of the ligand. With density functional theory calculations, we revealed that the enhanced magnetic anisotropy of Mn arising from the carbonitrile ligand provides a prototype for the engineering of the magnetism of quantum devices.
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http://dx.doi.org/10.1021/acs.nanolett.1c00545DOI Listing
April 2021

Green synthesis of olefin-linked covalent organic frameworks for hydrogen fuel cell applications.

Nat Commun 2021 Mar 31;12(1):1982. Epub 2021 Mar 31.

State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin, China.

Green synthesis of crystalline porous materials for energy-related applications is of great significance but very challenging. Here, we create a green strategy to fabricate a highly crystalline olefin-linked pyrazine-based covalent organic framework (COF) with high robustness and porosity under solvent-free conditions. The abundant nitrogen sites, high hydrophilicity, and well-defined one-dimensional nanochannels make the resulting COF an ideal platform to confine and stabilize the HPO network in the pores through hydrogen-bonding interactions. The resulting material exhibits low activation energy (E) of 0.06 eV, and ultrahigh proton conductivity across a wide relative humidity (10-90 %) and temperature range (25-80 °C). A realistic proton exchange membrane fuel cell using the olefin-linked COF as the solid electrolyte achieve a maximum power of 135 mW cm and a current density of 676 mA cm, which exceeds all reported COF materials.
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http://dx.doi.org/10.1038/s41467-021-22288-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012354PMC
March 2021

Robotic retroperitoneal versus transperitoneal inferior vena cava thrombectomy: right-sided cases with level I-II tumor thrombus.

J Endourol 2021 Mar 31. Epub 2021 Mar 31.

Chinese PLA General Hospital, Urology, 28 Fu Xing Road, Beijing 100853, China., Beijing, Beijing, China, 100853;

Objectives: To compare the perioperative hemodynamic consequences and oncology outcomes of robotic retroperitoneal versus transperitoneal inferior vena cava(IVC) thrombectomy(IVCT) for right renal cell carcinoma(RCC) with IVC tumor thrombus(IVCTT) that located below the first porta hepatis.

Patients And Methods: Between January 2018 and June 2019, 35 patients of right RCC with IVCTT that located below the first porta hepatis underwent robotic retroperitoneal IVCT(16 patients) or transperitoneal IVCT(19 patients). We have described the procedures of transperitoneal IVCT earlier. The main procedure of robotic retroperitoneal IVCT include circumferential dissection of the IVC, sequentially clamping subhepatic IVC, the left renal vein and the caudal IVC with vessel loops, IVCT, IVC repair, radical nephrectomy(RN). The following parameters were compared between the two groups: baselines characteristic, perioperative consequences and hemodynamic changes.

Results: Retroperitoneal and transperitoneal cohorts were comparable in terms of IVC thrombus length(3.2 vs 4.0 cm), IVC block time (18 vs 16 min, p=0.64), postoperative hospital stay (6 vs 6 days, p= 0.67), postoperative complications (0 vs 0), and recurrence or metastasis rate(0 vs 0) for patients with similar baseline characteristic. The retroperitoneal cohort tended to less blood loss (160 vs 240ml,p=0.024), shorter operative time(130 vs 145min,p=0.003), lower central venous pressure(p<0.05) and smaller diameter of IVC (p<0.05).

Conclusions: Robotic retroperitoneal RN and IVCT is feasible for patients of right RCC with IVCTT located below the first porta hepatis and is superior to transperitoneal IVCT in terms of bleeding control and operation time for skilled surgeons.
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http://dx.doi.org/10.1089/end.2021.0127DOI Listing
March 2021

The inhibitory activity of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on Candida albicans biofilms.

Photodiagnosis Photodyn Ther 2021 Mar 27;34:102271. Epub 2021 Mar 27.

Department of Stomatology, Huashan Hospital, Fudan University, No.12, Rd. Wulumuqi, Huashan Hospital, Shanghai, China. Electronic address:

Background: Biofilm-associated Candida albicans (C. albicans) infections are hard to cure due to their high levels of resistance to antifungal agents. Photodynamic therapy (PDT) is a promising approach for controlling infections caused by C. albicans. This study was designed to explore the inhibitory activity of PDT using 5-aminolevulinic acid (ALA) as photosensitizer against C. albicans biofilms.

Methods: C. albicans cell suspensions were incubated for 48 h to form mature biofilms. ALA solution was diluted to 15 mM and incubated with C. albicans biofilms for 5 h before irradiated by red light semiconductor laser under the light intensity of 300 J/cm and fluence rate of 100 mW/cm for 50 min. The inhibitory activity was evaluated from subcellular level, molecular level and transcriptional level using transmission electron microscopy (TEM) observation, flow cytometry analysis and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) assays, respectively.

Results: From subcellular level, the degraded content of the cytoplasm, nuclear condensation and mitochondrial swelling were observed after ALA-PDT. From molecular level, ALA-PDT resulted in 19.4 % cell apoptosis. From transcriptional level, ALA-PDT significantly reduced the mRNA expressions of hyphae-specific genes (HWP1 and ALS3) and long-term biofilm maintenance genes (UME6 and HGC1), whereas ALA or red light alone had no significant effect.

Conclusions: The inhibitory activity indicated that ALA-PDT may have the potential to serve as an antifungal strategy in eliminatingC. albicans biofilms.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102271DOI Listing
March 2021

Value of Apparent Diffusion Coefficient Histogram Analysis in the Differential Diagnosis of Nasopharyngeal Lymphoma and Nasopharyngeal Carcinoma Based on Readout-Segmented Diffusion-Weighted Imaging.

Front Oncol 2021 12;11:632796. Epub 2021 Mar 12.

Department of MRI, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: This study aims to explore the utility of whole-lesion apparent diffusion coefficient (ADC) histogram analysis for differentiating nasopharyngeal lymphoma (NPL) from nasopharyngeal carcinoma (NPC) following readout-segmented echo-planar diffusion-weighted imaging (RESOLVE sequence).

Methods: Thirty-eight patients with NPL and 62 patients with NPC, who received routine head-and-neck MRI and RESOLVE (b-value: 0 and 1,000 s/mm) examinations, were retrospectively evaluated as derivation cohort (February 2015 to August 2018); another 23 patients were analyzed as validation cohort (September 2018 to December 2019). The RESOLVE data were obtained from the MAGNETOM Skyra 3T MR system (Siemens Healthcare, Erlangen, Germany). Fifteen parameters derived from the whole-lesion histogram analysis (ADC, variance, skewness, kurtosis, ADC, ADC, ADC, ADC, ADC, ADC, ADC, ADC, ADC, ADC, and ADC) were calculated for each patient. Then, statistical analyses were performed between the two groups to determine the statistical significance of each histogram parameter. A receiver operating characteristic curve (ROC) analysis was conducted to assess the diagnostic performance of each histogram parameter for distinguishing NPL from NPC and further tested in the validation cohort; calibration of the selected parameter was tested with Hosmer-Lemeshow test.

Results: NPL exhibited significantly lower ADC, variance, ADC, ADC, ADC, ADC, ADC, ADC, ADC, ADC, ADC, ADC and ADC, when compared to NPC (all, < 0.05), while no significant differences were found on skewness and kurtosis. Furthermore, ADC revealed the highest diagnostic efficiency, followed by ADC and ADC. Optimal diagnostic performance (AUC = 0.790, sensitivity = 91.9%, and specificity = 63.2%) could be achieved when setting ADC = 1,485.0 × 10 mm/s as the threshold value. The predictive performance was maintained in the validation cohort (AUC = 0.817, sensitivity = 94.6%, and specificity = 56.2%).

Conclusion: Whole-lesion ADC histograms based on RESOLVE are effective in differentiating NPC from NPL.
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http://dx.doi.org/10.3389/fonc.2021.632796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996088PMC
March 2021

Inhibitor development of MTH1 via high-throughput screening with fragment based library and MTH1 substrate binding cavity.

Bioorg Chem 2021 May 10;110:104813. Epub 2021 Mar 10.

Institute of Plant Biology, National Taiwan University, Taipei 10617, Taiwan; Department of Life Science, National Taiwan University, Taipei 10617, Taiwan; Genome and Systems Biology Degree Program, Academia Sinica and National Taiwan University, Taipei 10617, Taiwan. Electronic address:

MutT Homolog 1 (MTH1) has been proven to hydrolyze oxidized nucleotide triphosphates during DNA repair. It can prevent the incorporation of wrong nucleotides during DNA replication and mitigate cell apoptosis. In a cancer cell, abundant reactive oxygen species can lead to substantial DNA damage and DNA mutations by base-pairing mismatch. MTH1 could eliminate oxidized dNTP and prevent cancer cells from entering cell death. Therefore, inhibition of MTH1 activity is considered to be an anti-cancer therapeutic target. In this study, high-throughput screening techniques were combined with a fragment-based library containing 2,313 compounds, which were used to screen for lead compounds with MTH1 inhibitor activity. Four compounds with MTH1 inhibitor ability were selected, and compound MI0639 was found to have the highest effective inhibition. To discover the selectivity and specificity of this action, several derivatives based on the MTH1 and MI0639 complex structure were synthesized. We compared 14 complex structures of MTH1 and the various compounds in combination with enzymatic inhibition and thermodynamic analysis. Nanomolar-range IC inhibition abilities by enzyme kinetics and K values by thermodynamic analysis were obtained for two compounds, named MI1020 and MI1024. Based on structural information and compound optimization, we aim to provide a strategy for the development of MTH1 inhibitors with high selectivity and specificity.
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http://dx.doi.org/10.1016/j.bioorg.2021.104813DOI Listing
May 2021

Long-term impact of ambient air pollution on preterm birth in Xuzhou, China: a time series study.

Environ Sci Pollut Res Int 2021 Mar 27. Epub 2021 Mar 27.

Department of Maternal Child and Adolescent Health, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.

Accumulating evidence witnesses the negative influence of air pollution on human health, but the relationship between air pollution and premature babies has been inconsistent. In this study, the association between weekly average concentration of air pollutants and preterm birth (PTB) was conducted in Xuzhou, a heavy industry city, in China. We constructed a distributed lag non-linear model (DLNM), an ecological study, to access the associations between ambient air pollutants and PTB in this study. Totally, 5408 premature babies were included, and the weekly average levels of PM, PM, SO, NO, O, and CO were 61.24, 110.21, 22.55, 40.55, 104.45, and 1.04 mg/m, respectively. We found that PM, PM, SO, and NO significantly increased the risk of PTB, and the susceptibility windows of these contaminants were the second trimester and third trimester (from 12 to 29 weeks). Every 10 μg/m increase of PM, PM, SO, and NO, the greatest relative risk (RR) values and 95% confidence interval (CI) on PTB were 1.0075 [95% CI, 1.0019-1.0131], 1.0053 [95% CI, 1.0014-1.0092], 1.0203 [95% CI, 1.0030-1.0379], and 1.0170 [95% CI, 1.0052-1.0289] in lag 16th, 18th, 19th, and 20th gestational weeks, respectively. No significant influence of O and CO were found on preterm birth. Subgroup analysis showed that the risk of premature delivery was higher for younger pregnant women and in warm season. This finding shows that prenatal exposure to ambient air pollutants is associated with preterm birth, and there existed an exposure window period.
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http://dx.doi.org/10.1007/s11356-021-13621-2DOI Listing
March 2021

Aldose reductase deficiency inhibits LPS-induced M1 response in macrophages by activating autophagy.

Cell Biosci 2021 Mar 26;11(1):61. Epub 2021 Mar 26.

Institute of Neurosciences, Fourth Military Medical University, Xi'an, 710032, China.

Macrophage M1 polarization mediates inflammatory responses and tissue damage. Recently, aldose reductase (AR) has been shown to play a critical role in M1 polarization in macrophages. However, the underlying mechanisms are unknown. Here, we demonstrated, for the first time, that AR deficiency repressed the induction of inducible nitric oxide synthase in lipopolysaccharide (LPS)-stimulated macrophages via activation of autophagy. This suppression was related to a defect in the inhibitor of nuclear factor κB (NF-κB) kinase (IKK) complex in the classical NF-κB pathway. However, the mRNA levels of IKKβ and IKKγ were not reduced in LPS-treated AR knockout (KO) macrophages, indicating that their proteins were downregulated at the post-transcriptional level. We discovered that LPS stimuli induced the recruitment of more beclin1 and increased autophagosome formation in AR-deficient macrophages. Blocking autophagy through 3-methyladenine and ammonium chloride treatment restored IKKβ and IKKγ protein levels and increased nitric oxide synthase production in LPS-stimulated AR-deficient macrophages. More assembled IKKβ and IKKγ underwent ubiquitination and recruited the autophagic adaptor p62 in LPS-induced AR KO macrophages, promoting their delivery to autophagosomes and lysosomes. Collectively, these findings suggest that AR deficiency is involved in the regulation of NF-κB signaling, and extends the role of selective autophagy in fine-tuned M1 macrophage polarization.
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http://dx.doi.org/10.1186/s13578-021-00576-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004403PMC
March 2021

Comparative proteomics reveals mechanisms that underlie insecticide resistance in Culex pipiens pallens Coquillett.

PLoS Negl Trop Dis 2021 Mar 25;15(3):e0009237. Epub 2021 Mar 25.

Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining, Shandong, P.R. China.

Mosquito control based on chemical insecticides is considered as an important element of the current global strategies for the control of mosquito-borne diseases. Unfortunately, the development of insecticide resistance of important vector mosquito species jeopardizes the effectiveness of insecticide-based mosquito control. In contrast to target site resistance, other mechanisms are far from being fully understood. Global protein profiles among cypermethrin-resistant, propoxur-resistant, dimethyl-dichloro-vinyl-phosphate-resistant and susceptible strain of Culex pipiens pallens were obtained and proteomic differences were evaluated by using isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography/tandem mass spectrometric analysis. A susceptible strain of Culex pipiens pallens showed elevated resistance levels after 25 generations of insecticide selection, through iTRAQ data analysis detected 2,502 proteins, of which 1,513 were differentially expressed in insecticide-selected strains compared to the susceptible strain. Finally, midgut differential protein expression profiles were analyzed, and 62 proteins were selected for verification of differential expression using iTRAQ and parallel reaction monitoring strategy, respectively. iTRAQ profiles of adaptation selection to three insecticide strains combined with midgut profiles revealed that multiple insecticide resistance mechanisms operate simultaneously in resistant insects of Culex pipiens pallens. Significant molecular resources were developed for Culex pipiens pallens, potential candidates were involved in metabolic resistance and reducing penetration or sequestering insecticide. Future research that is targeted towards RNA interference of the identified metabolic targets, such as cuticular proteins, cytochrome P450s, glutathione S-transferases and ribosomal proteins proteins and biological pathways (drug metabolism-cytochrome P450, metabolism of xenobiotics by cytochrome P450, oxidative phosphorylation, ribosome) could lay the foundation for a better understanding of the genetic basis of insecticide resistance in Culex pipiens pallens.
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http://dx.doi.org/10.1371/journal.pntd.0009237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993597PMC
March 2021

Activation of Nrf2/HO-1 signaling pathway attenuates ROS-mediated autophagy induced by silica nanoparticles in H9c2 cells.

Environ Toxicol 2021 Mar 25. Epub 2021 Mar 25.

Department of Toxicology, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University, Ji'nan, China.

Silica nanoparticles (SiNPs) as one of the most productive nano-powder, has been extensively applied in various fields. There has been increasing concern about the adverse effects of SiNPs on the health of ecological organisms and human. The potential cardiovascular toxicity of SiNPs and involved mechanisms remain elusive. Hence, in this study, we investigated the cardiovascular toxicity of SiNPs (60 nm) and explored the underlying mechanisms using H9c2 cardiomyocytes. Results showed that SiNPs induced oxidative stress and activated the Nrf2/HO-1 antioxidant pathway. Autophagy was also activated by SiNPs. Interestingly, N-acetyl-L-cysteine (NAC)attenuated autophagy after inhibiting reactive oxygen species (ROS). Meanwhile, down-regulation of Nrf2 enhanced autophagy. In summary, these data indicated that SiNPs induce autophagy in H9c2 cardiomyocytes through oxidative stress, and the Nrf2/HO-1 pathway has a negative regulatory effect on autophagy. This study provides new evidence for the cardiovascular toxicity of SiNPs and provides a reference for the safe use of nanomaterials in the future.
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http://dx.doi.org/10.1002/tox.23134DOI Listing
March 2021