Publications by authors named "Peng Chen"

2,861 Publications

  • Page 1 of 1

The Significance of Neutrophil Extracellular Traps in Colorectal Cancer and Beyond: From Bench to Bedside.

Front Oncol 2022 7;12:848594. Epub 2022 Jun 7.

Department of General Surgery, the First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Neutrophil extracellular traps (NETs), products of neutrophil death when exposed to certain stimuli, were first proposed as a type of response to bacterial infection in infectious diseases. Since then, extensive studies have discovered its involvement in other non-infectious inflammatory diseases including thromboembolism, autoimmune diseases, and cancer. Colorectal cancer (CRC) is one of the most common malignancies in the world. NET formation is closely associated with tumorigenesis, progression, and metastasis in CRC. Therefore, the application of NETs in clinical practice as diagnostic biomarkers, therapeutic targets, and prognostic predictors has a promising prospect. In addition, therapeutics targeting NETs are significantly efficient in halting tumor progression in preclinical cancer models, which further indicates its potential clinical utility in cancer treatment. This review focuses on the stimuli of NETosis, its pro-tumorigenic activity, and prospective clinical utility primarily in but not limited to CRC.
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http://dx.doi.org/10.3389/fonc.2022.848594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209713PMC
June 2022

Dihydrotanshinone I Enhances Cell Adhesion and Inhibits Cell Migration in Osteosarcoma U-2 OS Cells through CD44 and Chemokine Signaling.

Molecules 2022 Jun 9;27(12). Epub 2022 Jun 9.

Joint Research Centre for Engineering Biology, Zhejiang University-University of Edinburgh Institute, Zhejiang University, Haining 314400, China.

In the screening of novel natural products against cancer using an in vitro cancer cell model, we recently found that tanshinones from a traditional Chinese medicine, the rhizome of Bunge (Danshen), had potent effects on cell proliferation and migration. Especially for human osteosarcoma U-2 OS cells, tanshinones significantly enhanced the cell adherence, implying a possible role in cell adhesion and cell migration inhibition. In this work, therefore, we aimed to provide a new insight into the possible molecule mechanisms of dihydrotanshinone I, which had the strongest effects on cell adhesion among several candidate tanshinones. RNA-sequencing-based transcriptome analysis and several biochemical experiments indicated that there were comprehensive signals involved in dihydrotanshinone I-treated U-2 OS cells, such as cell cycle, DNA replication, thermogenesis, tight junction, oxidative phosphorylation, adherens junction, and focal adhesion. First, dihydrotanshinone I could potently inhibit cell proliferation and induce cell cycle arrest in the G0/G1 phase by downregulating the expression of , , , and cyclin E1 and upregulating the expression of p21. Second, it could significantly enhance cell adhesion on cell plates and inhibit cell migration, involving the hyaluronan CD44-mediated CXCL8-PI3K/AKT-FOXO1, IL6-STAT3-P53, and EMT signaling pathways. Thus, the increased expression of CD44 and lengthened protrusions around the cell yielded a significant increase in cell adhesion. In summary, these results suggest that dihydrotanshinone I might be an interesting molecular therapy for enhancing human osteosarcoma U-2 OS cell adhesion and inhibiting cell migration and proliferation.
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http://dx.doi.org/10.3390/molecules27123714DOI Listing
June 2022

Integrated Methylome and Transcriptome Analysis Provides Insights into the DNA Methylation Underlying the Mechanism of Cytoplasmic Male Sterility in Kenaf ( L.).

Int J Mol Sci 2022 Jun 20;23(12). Epub 2022 Jun 20.

Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning 530004, China.

Cytoplasmic male sterility (CMS) is widely exploited in hybrid seed production. Kenaf is an important fiber crop with high heterosis. The molecular mechanism of kenaf CMS remains unclear, particularly in terms of DNA methylation. Here, using the anthers of a kenaf CMS line (P3A) and its maintainer line (P3B), comparative physiological, DNA methylation, and transcriptome analyses were performed. The results showed that P3A had considerably lower levels of IAA, ABA, photosynthetic products and ATP contents than P3B. DNA methylome analysis revealed 650 differentially methylated genes (DMGs) with 313 up- and 337 down methylated, and transcriptome analysis revealed 1788 differentially expressed genes (DEGs) with 558 up- and 1230 downregulated genes in P3A compared with P3B. Moreover, 45 genes were characterized as both DEGs and DMGs, including , , , and . Many DEGs may be regulated by related DMGs based on methylome and transcriptome studies. These DEGs were involved in carbon metabolism, plant hormone signal transduction, the TCA cycle and the MAPK signaling pathway and were shown to be important for CMS in kenaf. These results provide new insights into the epigenetic mechanism of CMS in kenaf and other crops.
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http://dx.doi.org/10.3390/ijms23126864DOI Listing
June 2022

Nomogram to Predict Cognitive State Improvement after Deep Brain Stimulation for Parkinson's Disease.

Brain Sci 2022 Jun 9;12(6). Epub 2022 Jun 9.

Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China.

Purpose: Parkinson's disease (PD) is a common neurodegenerative disease, for which cognitive impairment is a non-motor symptom (NMS). Bilateral subthalamic nucleus deep brain stimulation (STN-DBS) is an effective treatment for PD. This study established a nomogram to predict cognitive improvement rate after STN-DBS in PD patients.

Methods: We retrospectively analyzed 103 PD patients who underwent STN-DBS. Patients were followed up to measure improvement in MoCA scores one year after surgery. Univariate and multivariate logistic regression analyses were used to identify factors affecting improvement in cognitive status. A nomogram was developed to predict this factor. The discrimination and fitting performance were evaluated by receiver operating characteristics (ROC) analysis, calibration diagram, and decision curve analysis (DCA).

Results: Among 103 patients, the mean improvement rate of the MoCA score was 37.3% and the median improvement rate was 27.3%, of which 64% improved cognition, 27% worsened cognition, and 8.7% remained unchanged. Logistic multivariate regression analysis showed that years of education, UPDRSIII drug use, MoCA Preop, and MMSE Preop scores were independent factors affecting the cognitive improvement rate. A nomogram model was subsequently developed. The C-index of the nomogram was 0.98 (95%CI, 0.97-1.00), and the area under the ROC was 0.98 (95%CI 0.97-1.00). The calibration plot and DCA demonstrated the goodness-of-fit between nomogram predictions and actual observations.

Conclusion: Our nomogram could effectively predict the possibility of achieving good cognitive improvement one year after STN-DBS in patients with PD. This model has value in judging the expected cognitive improvement of patients with PD undergoing STN-DBS.
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http://dx.doi.org/10.3390/brainsci12060759DOI Listing
June 2022

Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars.

Nat Commun 2022 Jun 23;13(1):3582. Epub 2022 Jun 23.

National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Naturally, haloacid dehalogenase superfamily phosphatases have been evolved with broad substrate promiscuity; however, strong specificity to a particular substrate is required for developing thermodynamically driven routes for manufacturing sugars. How to alter the intrinsic substrate promiscuity of phosphatases and fit the "one enzyme-one substrate" model remains a challenge. Herein, we report the structure-guided engineering of a phosphatase, and successfully provide variants with tailor-made preference for three widespread phosphorylated sugars, namely, glucose 6-phosphate, fructose 6-phosphate, and mannose 6-phosphate, while simultaneously enhancement in catalytic efficiency. A 12000-fold switch from unfavorite substrate to dedicated one is generated. Molecular dynamics simulations reveal the origin of improved activity and substrate specificity. Furthermore, we develop four coordinated multienzyme systems and accomplish the conversion of inexpensive sucrose and starch to fructose and mannose in excellent yield of 94-96%. This innovative sugar-biosynthesis strategy overcomes the reaction equilibrium of isomerization and provides the promise of high-yield manufacturing of other monosaccharides and polyols.
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http://dx.doi.org/10.1038/s41467-022-31371-8DOI Listing
June 2022

Low serum uric acid levels are associated with incidence and severity in trigeminal neuralgia.

Neurol Sci 2022 Jun 23. Epub 2022 Jun 23.

Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Background: Uric acid is a natural antioxidant, and low levels of uric acid have been reported to be a potential risk factor in the development of nervous system diseases. Herein, we investigated whether uric acid levels play a role in trigeminal neuralgia (TN).

Methods: We conducted a cohort study to compare the serum uric acid levels of patients with TN and healthy controls. We also analyzed the impact of uric acid levels on the risk of TN and symptom severity.

Results: In comparison to control participants (n = 133), uric acid levels were remarkably decreased in patients with TN (n = 181). Uric acid (OR = 0.989; 95% CI 0.986-0.993; P < 0.001) was also determined as a protective factor against TN based on multivariate logistic regression models. Furthermore, nonlinear relationships between serum uric acid levels and TN incidence rate and between that and the Barrow Neurological Institute (BNI) grading were observed.

Conclusions: Our study is the first to show a relationship between serum uric acid levels and TN. Specifically, low serum uric acid levels were associated with an increased risk of TN and more severe clinical symptoms. We expect that these findings will provide new insights into the prevention and treatment of TN.
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http://dx.doi.org/10.1007/s10072-022-06223-4DOI Listing
June 2022

Sequential posterior interosseous artery perforator flap for reconstruction of dorsal hand defects.

Clin Anat 2022 Jun 23. Epub 2022 Jun 23.

Laboratory of Basic Medicine, The 900th Hospital of PLA, Fuzhou, P.R. China.

Introduction: Dorsal hand defects accompanied by exposure of bones and tendons remain a huge challenge for plastic surgeons. The pedicled perforator flaps have unique advantages in resurfacing the defects. This study aimed to investigate the clinical efficacy of the sequential posterior interosseous artery perforator flap for repairing dorsal hand defects.

Materials And Methods: This study was composed of an anatomical study and clinical application. Anatomically, thirty adult upper limb specimens injected with red latex were dissected, the perforators from the branches of the posterior interosseous artery were observed in the dorsal forearm, and the sequential flap based on them was designed based on the anatomical characteristics. Clinically, nine cases of soft tissue defects on the dorsum of the hand were treated by this flap.

Results: Anatomically, the posterior interosseous artery divided into an ascending branch and a descending branch, and the descending branch traveled 1.0±0.3 cm down to divide into the ulnar and radial terminal branches. The ulnar terminal branch presented constantly, and the radial terminal branch had an occurrence rate of 93.33%. Clinically, all flaps survived completely and possessed a soft texture and satisfactory appearance, as well as a nonbloated pedicle, and the donor region had a natural color and appearance with only a linear scar left behind.

Conclusions: The sequential posterior interosseous artery perforator flap could become a useful option for repairing dorsal hand defects, as it has the advantages of being easy to perform without sacrificing the main vessels and avoiding donor area skin grafting. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/ca.23922DOI Listing
June 2022

Genomic status of yellow-breasted bunting following recent rapid population decline.

iScience 2022 Jul 2;25(7):104501. Epub 2022 Jun 2.

Chengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, P. R. China.

Global biodiversity is facing serious threats. However, knowledge of the genomic consequences of recent rapid population declines of wild organisms is limited. Do populations experiencing recent rapid population decline have the same genomic status as wild populations that experience long-term declines? Yellow-breasted Bunting () is a critically endangered species that has been experiencing a recent rapid population decline. To answer the question, we assembled and annotated the whole genome of Yellow-breasted Bunting. Furthermore, we found high genetic diversity, low linkage disequilibrium, and low proportion of long runs of homozygosity in Yellow-breasted Bunting, suggesting that the populations following recent rapid declines have different genomic statuses from the population that experienced long-term population decline.
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http://dx.doi.org/10.1016/j.isci.2022.104501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207672PMC
July 2022

Numerical study on high-frequency effect of rail corrugation on subway-induced environmental vibrations.

Environ Sci Pollut Res Int 2022 Jun 21. Epub 2022 Jun 21.

School of Civil Engineering, Beijing Jiaotong University, Beijing, 100044, China.

Rail corrugation is a common phenomenon in railway engineering, but its high-frequency effects on environmental vibrations are neglected in most previous research. Therefore, a hybrid numerical method was proposed in this paper to analyze subway-induced ground vibrations, especially in the high-frequency range caused by rail corrugation. The analysis model composed of a three-dimensional (3D) load generation subsystem and a two-dimensional (2D) wave propagation subsystem was established based on the vehicle-track coupling method and finite element method, and validated by the measured data. Then the high-frequency effects under different tunnel depths and rail fasteners were further studied. The results show that high-frequency vibrations propagate radially from the tunnel wall to the surrounding soil and transmit to the ground by the dominant path under different tunnel depths. The increase of tunnel depths could result in more serious high-frequency effects in the vibration amplification region. When the depth changes from 17 to 29 m, the 250 Hz ground vibration at around 30 m away from the track increases by 5.6 dB. Besides, it was found that in the commonly used range, the reduction of fastener stiffness can effectively eliminate high-frequency ground vibrations, while there is a significant nonlinear relationship between fastener damping and high-frequency vibration. The findings of this paper could provide references for parameter design in subway construction and rail corrugation remediation, and help create better living environments.
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http://dx.doi.org/10.1007/s11356-022-21264-0DOI Listing
June 2022

Construction and Application of YOLOv3-Based Diatom Identification Model of Scanning Electron Microscope Images.

Fa Yi Xue Za Zhi 2022 Feb;38(1):46-52

Department of Forensic Medicine, Nanjing Medical University, Nanjing 211126, China.

Objectives: To construct a YOLOv3-based model for diatom identification in scanning electron microscope images, explore the application performance in practical cases and discuss the advantages of this model.

Methods: A total of 25 000 scanning electron microscopy images were collected at 1 500× as an initial image set, and input into the YOLOv3 network to train the identification model after experts' annotation and image processing. Diatom scanning electron microscopy images of lung, liver and kidney tissues taken from 8 drowning cases were identified by this model under the threshold of 0.4, 0.6 and 0.8 respectively, and were also identified by experts manually. The application performance of this model was evaluated through the recognition speed, recall rate and precision rate.

Results: The mean average precision of the model in the validation set and test set was 94.8% and 94.3%, respectively, and the average recall rate was 81.2% and 81.5%, respectively. The recognition speed of the model is more than 9 times faster than that of manual recognition. Under the threshold of 0.4, the mean recall rate and precision rate of diatoms in lung tissues were 89.6% and 87.8%, respectively. The overall recall rate in liver and kidney tissues was 100% and the precision rate was less than 5%. As the threshold increased, the recall rate in all tissues decreased and the precision rate increased. The 1 score of the model in lung tissues decreased with the increase of threshold, while the 1 score in liver and kidney tissues with the increase of threshold.

Conclusions: The YOLOv3-based diatom electron microscope images automatic identification model works at a rapid speed and shows high recall rates in all tissues and high precision rates in lung tissues under an appropriate threshold. The identification model greatly reduces the workload of manual recognition, and has a good application prospect.
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http://dx.doi.org/10.12116/j.issn.1004-5619.2021.410903DOI Listing
February 2022

An Eco-Friendly Conversion of Aquaculture Suspended Solid Wastes Into High-Quality Fish Food by Improving Poly-β-Hydroxybutyrate Production.

Front Physiol 2022 26;13:797625. Epub 2022 May 26.

Yancheng Institute of Technology, Yancheng, China.

The aquaculture industry is vital in providing a valuable protein food source for humans, but generates a huge amount of solid and dissolved wastes that pose great risks to the environment and aquaculture sustainability. Suspended solids (in short SS), one of the aquaculture wastes, are very difficult to be treated due to their high organic contents. The bioconversion from wastewater, food effluents, and activated sludge into poly-β-hydroxybutyrate (PHB) is a sustainable alternative to generate an additional income and could be highly attractive to the agricultural and environmental management firms. However, little is known about its potential application in aquaculture wastes. In the present study, we first determined that 7.2% of SS was PHB. Then, the production of PHB was increased two-fold by the optimal fermentation conditions of wheat bran and microbial cocktails at a C/N ratio of 12. Also, the PHB-enriched SS showed a higher total ammonia nitrogen removal rate. Importantly, we further demonstrated that the PHB-enriched SS as a feed could promote fish growth and up-regulate the expression of the immune-related genes. Our study developed an eco-friendly and simple approach to transforming problematic SS wastes into PHB-enriched high-quality food for omnivorous fish, which will increase the usage efficiency of SS and provide a cheaper diet for aquatic animals.
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http://dx.doi.org/10.3389/fphys.2022.797625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205610PMC
May 2022

12-Deoxyphorbol-13-Hexadecanoate Abrogates OVX-Induced Bone Loss in Mice and Osteoclastogenesis Inhibiting ROS Level and Regulating RANKL-Mediated NFATc1 Activation.

Front Pharmacol 2022 3;13:899776. Epub 2022 Jun 3.

Department of Orthopaedics, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.

Osteoporosis is a major health problem in the elderly. Almost every bone can fracture due to the increased bone fragility in osteoporosis, posing a major challenge to public health. 12-Deoxyphorbol-13-hexadecanoate (DHD), one of the main bioactive components of L. (Lang Du), is considered to have antitumor, antibacterial, and antifungal properties. However, the role of DHD in osteoporosis is still elusive. In this study, we demonstrated for the first time that DHD inhibits the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and bone resorption in a dose- and time-dependent manner without exhibiting cytotoxicity . Mechanistically, we found that DHD not only represses the expression of osteoclasts marker genes by suppressing RANKL-induced mitogen-activated protein kinase (MAPK) and calcium signaling pathways but also scavenges reactive oxygen species (ROS) through enhancing cytoprotective enzymes expression. Furthermore, DHD inhibits the activation of nuclear factor of activated T cells 1 (NFATc1) during RANKL-induced osteoclasts formation. Preclinical studies revealed that DHD protects against bone loss in ovariectomy (OVX) mice. In sum, our data confirmed that DHD could potentially inhibit osteoclastogenesis by abrogating RANKL-induced MAPK, calcium, and NFATc1 signaling pathways and promoting the expression of ROS scavenging enzymes, thereby preventing OVX-induced bone loss. Thus, DHD may act as a novel therapeutic agent to manage osteoporosis.
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http://dx.doi.org/10.3389/fphar.2022.899776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204068PMC
June 2022

Global Context-Aware-Based Deformable Residual Network Module for Precise Pest Recognition and Detection.

Front Plant Sci 2022 2;13:895944. Epub 2022 Jun 2.

Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.

An accurate and robust pest detection and recognition scheme is an important step to enable the high quality and yield of agricultural products according to integrated pest management (IPM). Due to pose-variant, serious overlap, dense distribution, and interclass similarity of agricultural pests, the precise detection of multi-classes pest faces great challenges. In this study, an end-to-end pest detection algorithm has been proposed on the basis of deep convolutional neural networks. The detection method adopts a deformable residual network to extract pest features and a global context-aware module for obtaining region-of-interests of agricultural pests. The detection results of the proposed method are compared with the detection results of other state-of-the-art methods, for example, RetinaNet, YOLO, SSD, FPN, and Cascade RCNN modules. The experimental results show that our method can achieve an average accuracy of 77.8% on 21 categories of agricultural pests. The proposed detection algorithm can achieve 20.9 frames per second, which can satisfy real-time pest detection.
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http://dx.doi.org/10.3389/fpls.2022.895944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201688PMC
June 2022

Immune Subtypes in LUAD Identify Novel Tumor Microenvironment Profiles With Prognostic and Therapeutic Implications.

Front Immunol 2022 3;13:877896. Epub 2022 Jun 3.

Department of Thoracic oncology surgery, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, China.

The six transcriptomic immune subtypes (ISs) (C1 - C6) were reported to have complex and different interplay between TME and cancer cells in TCGA (The Cancer Genome Atlas) pan-cancer cohort. Our study specifically explored how the consequence of interplay determines the prognosis and the response to therapy in LUAD cohorts. Clinical and molecular information of LUAD patients were from TCGA and Gene Expression Omnibus (GEO). The immune cell populations and gene/pathway enrichment analysis were performed to explore the molecular differences among the C3 IS and other ISs in the LUAD population. The proportion of C3 inflammatory IS was identified as the most common IS in both TCGA ( 457) and GEO ( 901) cohorts. The C3 IS was also found to be the most accurate prognostic subtype, which was associated with significantly longer OS (p <0.001) and DFS (p <0.001). The C3 IS presented higher levels of CD8 T, M1 macrophage, and myeloid dendritic cells, while lower levels of M2 macrophages and cancer-associated fibroblast cells. Moreover, the C3 subtype was enriched in the antigen process and presenting, interferon-gamma response, T cell receptor signaling, and natural killer cell-mediated cytotoxicity pathways than C1/C2. In contrast, the C1/C2 presented greater activation of pathways related to the cell cycles, DNA repair, and p53 signaling pathways. The immune-related C3 IS had a great ability to stratify the prognosis of LUAD, providing clues for further pathogenic research. This classification might help direct precision medicine screenings of LUAD patients, thus possibly improving their prognoses.
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http://dx.doi.org/10.3389/fimmu.2022.877896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203850PMC
June 2022

Global Trends in Research of Lipid Metabolism in T lymphocytes From 1985 to 2022: A Bibliometric Analysis.

Front Immunol 2022 26;13:884030. Epub 2022 May 26.

Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Lipids are involved in both energy metabolism and signaling transduction. Abnormal lipid metabolism in T cells is associated with the differentiation, longevity and activity of T cells, which has received increasing concern since its firstly reported in 1985. To evaluate the trends of lipid metabolism in T cells and map knowledge structure, we employed bibliometric analysis. A total of 286 related publications obtained from the Web of Science Core Collection published between 1985 and 2022 were analyzed using indicators of publication and citation metrics, countries, institutes, authors, cited references and key words. The present research status, the global trends and the future development directions in lipid metabolism and T cells were visualized and discussed. In summary, this study provides a comprehensive display on the field of lipid metabolism in T cells, which will help researchers explore lipid metabolism in T cells more effectively and intuitively.
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http://dx.doi.org/10.3389/fimmu.2022.884030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204382PMC
June 2022

Exosome-derived FGD5-AS1 promotes tumor-associated macrophage M2 polarization-mediated pancreatic cancer cell proliferation and metastasis.

Cancer Lett 2022 Jun 16:215751. Epub 2022 Jun 16.

Department of Hepatic-Biliary-Pancreatic Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, PR China. Electronic address:

Inflammatory molecules and exosomes are crucial for signal transduction between tumor-associated macrophages and tumor cells. IL-6, a key inflammatory molecule secreted by M2 macrophages after polarization, can mediate malignant progression of pancreatic cancer (PC). However, the functions and mechanisms of IL-6 and tumor-derived exosomes in tumor-associated macrophages and PC remain unclear. Transcriptome chip and quantitative reverse transcription PCR experiments indicated that FGD5-AS1 induced IL-6 and high FGD5-AS1 expression correlated with the poor prognosis in PC patients. RNA pulldown, mass spectrometry, and dual luciferase reporter assays were used to identify the mechanism of exosomal FGD5-AS1 in promoting PC progression and M2 macrophage polarization. FGD5-AS1 exerted cancer-promoting functions when co-cultured with M2 macrophages. PC-derived exosomal FGD5-AS1 stimulated M2 macrophage polarization by activating STAT3/NF-κB pathway. FGD5-AS1 interacts with p300, resulting in STAT3 acetylation, thus promoting nuclear localization and transcriptional activity of STAT3/NF-κB. These data indicated that PC cells generate FGD5-AS1-rich exosomes, which cause M2 macrophage polarization to promote the malignant behaviors of PC cells. Targeting exosomal FGD5-AS1 may provide a potential diagnosis and treatment strategy for PC.
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http://dx.doi.org/10.1016/j.canlet.2022.215751DOI Listing
June 2022

MG53 Inhibits Necroptosis Through Ubiquitination-Dependent RIPK1 Degradation for Cardiac Protection Following Ischemia/Reperfusion Injury.

Front Cardiovasc Med 2022 31;9:868632. Epub 2022 May 31.

Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, United States.

Rationale: While reactive oxygen species (ROS) has been recognized as one of the main causes of cardiac injury following myocardial infarction, the clinical application of antioxidants has shown limited effects on protecting hearts against ischemia-reperfusion (I/R) injury. Thus, the precise role of ROS following cardiac injury remains to be fully elucidated.

Objective: We investigated the role of mitsugumin 53 (MG53) in regulating necroptosis following I/R injury to the hearts and the involvement of ROS in MG53-mediated cardioprotection.

Methods And Results: Antioxidants were used to test the role of ROS in MG53-mediated cardioprotection in the mouse model of I/R injury and induced human pluripotent stem cells (hiPSCs)-derived cardiomyocytes subjected to hypoxia or re-oxygenation (H/R) injury. Western blotting and co-immunoprecipitation were used to identify potential cell death pathways that MG53 was involved in. CRISPR/Cas 9-mediated genome editing and mutagenesis assays were performed to further identify specific interaction amino acids between MG53 and its ubiquitin E3 ligase substrate. We found that MG53 could protect myocardial injury inhibiting the necroptosis pathway. Upon injury, the generation of ROS in the infarct zone of the hearts promoted interaction between MG53 and receptor-interacting protein kinase 1 (RIPK1). As an E3 ubiquitin ligase, MG53 added multiple ubiquitin chains to RIPK1 at the sites of K316, K604, and K627 for proteasome-mediated RIPK1 degradation and inhibited necroptosis. The application of N-acetyl cysteine (NAC) disrupted the interaction between MG53 and RIPK1 and abolished MG53-mediated cardioprotective effects.

Conclusions: Taken together, this study provided a molecular mechanism of a potential beneficial role of ROS following acute myocardial infarction. Thus, fine-tuning ROS levels might be critical for cardioprotection.
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http://dx.doi.org/10.3389/fcvm.2022.868632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193967PMC
May 2022

ACS Chemical Biology "Young Investigator" Special Issue.

ACS Chem Biol 2022 Jun 16. Epub 2022 Jun 16.

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http://dx.doi.org/10.1021/acschembio.2c00329DOI Listing
June 2022

This is in response to Letter to the Editor: "No Association Between Nonalcoholic Fatty Liver Disease and Severe COVID-19? Something We Might Ignore" by Hui Gao and Lei Xu.

Clin Gastroenterol Hepatol 2022 Jun 9. Epub 2022 Jun 9.

Department of Pathology, College of Basic Medical Sciences, Jilin University, Changchun, China; Department of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, China. Electronic address:

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http://dx.doi.org/10.1016/j.cgh.2022.05.019DOI Listing
June 2022

Comprehensive Study of the Current-Induced Spin-Orbit Torque Perpendicular Effective Field in Asymmetric Multilayers.

Nanomaterials (Basel) 2022 May 31;12(11). Epub 2022 May 31.

Department of Electrical Engineering, University of California, Los Angeles, CA 90095, USA.

The spin-orbit torques (SOTs) in the heavy metal (HM)/ferromagnetic metal (FM) structure hold promise for next-generation low-power and high-density spintronic memory and logic applications. For the SOT switching of a perpendicular magnetization, an external magnetic field is inevitable for breaking the mirror symmetry, which is not practical for high-density nanoelectronics applications. In this work, we study the current-induced field-free SOT switching and SOT perpendicular effective field (Hzeff) in a variety of laterally asymmetric multilayers, where the asymmetry is introduced by growing the FM layer in a wedge shape. We show that the design of structural asymmetry by wedging the FM layer is a universal scheme for realizing field-free SOT switching. Moreover, by comparing the FM layer thickness dependence of (Hzeff) in different samples, we show that the efficiency (Hzeff/, is the current density) is sensitive to the HM/FM interface and the FM layer thickness. The sign of for thin FM thicknesses is related to the spin Hall angle () of the HM layer attached to the FM layer. changes its sign with the thickness of the FM layer increasing, which may be caused by the thickness dependence of the work function of FM. These results show the possibility of engineering the deterministic field-free switching by combining the symmetry breaking and the materials design of the HM/FM interface.
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http://dx.doi.org/10.3390/nano12111887DOI Listing
May 2022

Role of Zirconia in Oxide-Zeolite Composite for Thiolation of Methanol with Hydrogen Sulfide to Methanethiol.

Nanomaterials (Basel) 2022 May 25;12(11). Epub 2022 May 25.

Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.

In this paper, the molecular sieve NaZSM-5 was modified with zirconium dioxide (ZrO) by a hydrothermal coating process and other methods. By comparing the effects of the crystal phase structure of ZrO and the compositing method on the physicochemical properties and catalytic performance of the obtained composites, the structure-performance relationship of these composite catalysts was revealed. The results indicate that in the hydrothermal system used for the preparation of NaZSM-5, Zr is more likely to dissolve from m-ZrO than from t-ZrO, which can subsequently enter the molecular sieve, causing a greater degree of desiliconization of the framework. The larger specific surface area (360 m/g) and pore volume (0.52 cm/g) of the m-ZrO/NaZSM-5 composite catalyst increase the exposure of its abundant acidic (0.078 mmol/g) and basic (0.081 mmol/g) active centers compared with other composites. Therefore, this catalyst exhibits a shorter induction period and better catalytic performance. Furthermore, compared with the impregnation method and mechanochemical method, the hydrothermal coating method produces a greater variety of acid-base active centers in the composite catalyst due to the hydrothermal modifying effect.
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http://dx.doi.org/10.3390/nano12111803DOI Listing
May 2022

Analysis of Silver Nanoparticles for the Treatment and Prevention of Nucleopolyhedrovirus Affecting .

Int J Mol Sci 2022 Jun 5;23(11). Epub 2022 Jun 5.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.

nucleopolyhedrovirus (BmNPV) causes major economic losses in sericulture. A number of agents have been employed to treat viral diseases. Silver nanoparticles (AgNPs) have wide applications in biomedical fields due to their unique properties. The anti-BmNPV effect of AgNPs has been evaluated, however, there are insufficient studies concerning its toxicity to other organisms and the environment. We chemically synthesized biocompatible BSA-AgNPs with a diameter range of 2-4 nm and characterized their physical properties. The toxicity of AgNPs towards cells and larvae with different concentrations was examined; the results indicated a biofriendly effect on cells and larvae within specific concentration ranges. The SEM observation of the surface of BmNPV after treatment with AgNPs suggested that AgNPs could destroy the polyhedral structure, and the same result was obtained by Coomassie blue staining. Further assays confirmed the weakened virulence of AgNPs-treated BmNPV toward cells and larvae. AgNPs also could effectively inhibit the replication of BmNPV in infected cells and larvae. In summary, our research provides valuable data for the further development of AgNPs as an antiviral drug for sericulture.
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http://dx.doi.org/10.3390/ijms23116325DOI Listing
June 2022

Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and YT013.

Front Bioeng Biotechnol 2022 23;10:898240. Epub 2022 May 23.

School of Pharmacy, Lanzhou University, Lanzhou, China.

Cancer is second only to heart disease as a cause of death, despite improvements in its early diagnosis and precision medicine. Due to the limitations of commonly used anticancer methods such as surgery, radiotherapy and chemotherapy, biological therapy, especially probiotics such as lactic acid bacteria, has received widespread attention. has been proven to inhibit the proliferation of a variety of cancer cells. In this work, the effects of the cell-free culture supernatant of serofluid dish (CCS1) and the cell-free culture supernatant of YT013 (CCS2) isolated from serofluid dish on AGS, HCT116, HepG2 and PANC-1 cells were investigated. Based on the CCK-8 assay, CCS1 and CCS2 were shown to suppress the growth of cancer cells in a concentration-dependent manner. The IC values of CCS2 of AGS, HCT116, HepG2 and PANC-1 cells were 346.51 ± 35.28, 1207.69 ± 333.18, 650.94 ± 123.78 and 808.96 ± 126.27 μg/ml, respectively. In addition, the results of fluorescence microscopy showed that CCS2 changed cell morphology and treated with CCS2 (200, 400 and 800 μg/ml) for 48 h, AGS cell apoptosis was quantitatively surveyed by flow cytometry, showing 25.0, 34.1, and 42.6% total apoptotic cells. Moreover, western blotting confirmed that BAX, BAD and Caspase-3/8/9 were significantly upregulated and that BCL-2 was significantly downregulated in AGS cells treated with CCS2. These results indicated that CCS2 might lead to apoptosis via the endogenous mitochondrial apoptotic pathway. In summary, YT013 may be considered a good candidate for anticancer therapies.
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http://dx.doi.org/10.3389/fbioe.2022.898240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168531PMC
May 2022

Cesium acetate-assisted crystallization for high-performance inverted CsPbIperovskite solar cells.

Nanotechnology 2022 Jun 24;33(37). Epub 2022 Jun 24.

National Laboratory of Solid State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences, Frontiers Science Center for Critical Earth Material Cycling, Nanjing University, Nanjing 210023, People's Republic of China.

Efficient inverted (p-i-n) type CsPbIperovskite solar cells (PSCs) have revealed promising applications due to their excellent thermal and photostability. Regulating the nucleation and crystallization of perovskite film is an important route to improving the performance of CsPbIPSCs. Herein, we explored cesium acetate (CsAc) as additive to manipulate the crystallization process of CsPbIperovskite films. By involving in the intermediate phase DMACsPbIAcof perovskite, the pseudo-halide acetate (Ac) can retard the ion exchange reaction between DMAand Cs, leading to a perovskite with dense morphology, low defect density, and a long carrier lifetime. As a result, the optimal CsPbIPSCs yielded a high power conversion efficiency of 18.3%. Moreover, the encapsulated devices showed excellent operational stability and the devices retained their initial performance following 500 h of operation at the maximum power point under one-sun illumination in ambient conditions.
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http://dx.doi.org/10.1088/1361-6528/ac76d5DOI Listing
June 2022

Spin-Decoupled Transflective Spatial Light Modulations Enabled by a Piecewise-Twisted Anisotropic Monolayer.

Adv Sci (Weinh) 2022 Jun 4:e2202424. Epub 2022 Jun 4.

National Laboratory of Solid State Microstructures, Key Laboratory of Intelligent Optical Sensing and Manipulation, and College of Engineering and Applied Sciences, Nanjing University, Nanjing, 210023, China.

Wavefront control lies at the heart of modern optics. Metasurfaces with specifically tailored resonators can encode different phases to two orthogonal polarization components, but suffer from wavelength-dependent efficiency, sophisticated fabrication, and limited size. Liquid crystals, another excellent candidate for planar optics, are restricted to spin-coupled conjugated phase modulations. Planar optics with spin-decoupled functions is expected to release the multifunctionality of modern optics. Here, a spin-decoupled transflective spatial light modulator is presented with a piecewise-twisted anisotropic monolayer. The phases of reflected and transmitted light can be independently customized by preprogramming the initial orientations of the periodic helix and mirror-symmetric dual-twist configuration, respectively. A transflective orbital angular momentum encoder and decoder is demonstrated, which is simultaneously compatible with different multiplexing techniques. This work releases the multifunctionality of advanced planar optics and may upgrade existing devices in optical informatics.
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http://dx.doi.org/10.1002/advs.202202424DOI Listing
June 2022

Circular Nonuniform Electric Field Gel Electrophoresis for the Separation and Concentration of Nanoparticles.

Anal Chem 2022 Jun 2;94(23):8474-8482. Epub 2022 Jun 2.

Department of Chemistry, Yanbian University, Park Road 977, Yanji 133002, Jilin, China.

A circular nonuniform electric field strategy coupled with gel electrophoresis was proposed to control the precise separation and efficient concentration of nano- and microparticles. The circular nonuniform electric field has the feature of exponential increase in the electric field intensity along the radius, working with three functional zones of migration, acceleration, and concentration. The distribution form of electric field lines is regulated in functional zones to control the migration behaviors of particles for separation and concentration by altering the relative position of the ring electrode (outside) and rodlike electrode (inner). The circular nonuniform electric field promotes the target-type and high-precision separation of nanoparticles based on the difference in charge-to-size ratio. The concentration multiple of nanoparticles is also controlled randomly with the alternation of radius, taking advantage of vertical extrusion and concentric converging of the migration path. This work provides a brand new insight into the simultaneous separation and concentration of particles and is promising for developing a versatile tool for the separation and preparation of various samples instead of conventional methods.
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http://dx.doi.org/10.1021/acs.analchem.2c01313DOI Listing
June 2022

Coordination Chemistry Engineered Polymeric Carbon Nitride Photoanode with Ultralow Onset Potential for Water Splitting.

Angew Chem Int Ed Engl 2022 Jun 1. Epub 2022 Jun 1.

The University of Queensland, School of Chemical Engineering and AIBN, St Lucia, 4072, Brisbane, AUSTRALIA.

CConstruction of an intimate film/substrate interface is of great importance for a photoelectrode to achieve efficient photoelectrochemical performance. Inspired by coordination chemistry, a polymeric carbon nitride (PCN) film is intimately grown on a titanium (Ti) coated substrate by an in-situ thermal condensation process. The as-prepared PCN photoanode exhibits a record low onset potential (Eonset) of -0.38 V versus reversible hydrogen electrode (RHE) and a decent photocurrent density of 242 μA/cm2 at 1.23 VRHE for water splitting. Detailed characterization confirms that the origin of the ultralow onset potential is mainly attributed to the substantially reduced interfacial resistance between the Ti coated substrate and the PCN film benefited from the constructed interfacial sp2 N→Ti coordination bonds. For the first time, the ultralow onset potential enables the PCN photoanode to drive water splitting without external bias with a stable photocurrent density of ~9 μA/cm2 up to 1 hour.
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http://dx.doi.org/10.1002/anie.202204407DOI Listing
June 2022

Corneal Epithelium-Derived Netrin-1 Alleviates Dry Eye Disease via Regulating Dendritic Cell Activation.

Invest Ophthalmol Vis Sci 2022 06;63(6)

Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, China.

Purpose: To investigate the expression of corneal epithelium-derived netrin-1 (NTN-1) and its immunoregulatory function in dry eye disease (DED) using a DED mouse model.

Methods: We generated DED mouse models with desiccating stress under scopolamine treatment. RNA sequencing was performed to identify differentially expressed genes (DEGs) in the corneal epithelium of DED mice. NTN-1 expression was analyzed via real-time PCR, immunofluorescence staining, and immunoblotting. The DED mice were then treated with recombinant NTN-1 or neutralizing antibodies to investigate the severity of the disease, dendritic cell (DC) activation, and inflammatory cytokine expression.

Results: A total of 347 DEGs (292 upregulated and 55 downregulated) were identified in the corneal epithelium of DED mice: corneal epithelium-derived NTN-1 expression was significantly decreased in DED mice compared to that in control mice. Topical recombinant NTN-1 application alleviated the severity of the disease, accompanied by restoration of tear secretion and goblet cell density. In addition, NTN-1 decreased the number of DCs, inhibited the activation of the DCs and Th17 cells, and reduced the expression of inflammatory factors in DED mice. In contrast, blocking endogenous NTN-1 activity with an anti-NTN-1 antibody aggravated the disease, enhanced DC activation, and upregulated the inflammatory factors in the conjunctivae of DED mice.

Conclusions: We identified decreased NTN-1 expression in the corneal epithelium of DED mice. Our findings elucidate the role of NTN-1 in alleviating DED and impeding DC activation, thereby indicating its therapeutic potential in suppressing ocular inflammation in DED.
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http://dx.doi.org/10.1167/iovs.63.6.1DOI Listing
June 2022

Anti-programmed death 1 antibody in the treatment of coexistent and lung cancer: A case report.

World J Clin Cases 2022 Apr;10(12):3801-3807

Department of Thoracic Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.

Background: Nontuberculous mycobacterium (NTM) refers to all mycobacteria except and , also known as environmental Mycobacterium. The patients with lung cancer and NTM are somewhat special; the two diseases are inevitably influenced by each other. It brings difficulties and challenges to the choice of treatment. Recently, cancer immunotherapy has been considered one of the pillars for the treatment of lung cancer. However, the clinical experience in the application of immune checkpoint inhibitors is scarce for lung cancer patients with pulmonary tuberculosis, and lung cancer with NTM is even more rare. Although it ameliorates lung cancer, immunotherapy with immune checkpoint inhibitors presents complications of infectious diseases, including tuberculosis and NTM.

Case Summary: A 61-year-old male patient visited a doctor in May 2019. His admitting diagnoses were: (1) Cancer of the left lung with a pathological diagnosis of poorly differentiated non-small cell carcinoma, likely poorly differentiated adenocarcinoma, clinical stage IIIb (T3N3M0); and (2) () infection. We chose to proceed with pembrolizumab treatment. After two treatment cycles, a chest computed tomography scan showed a new irregular subpleural mass in the anterior segment of the left upper lobe of the lung, a reduction in the mediastinal enlarged lymph node, and no other obvious changes. Next, an ultrasound-guided biopsy of the new tumor was performed. Pathological examination showed that a large number of carbon particles were deposited in the alveolar tissue with histiocyte reaction and multinucleated giant cell formation. The tuberculosis (TB) specialist suggested that anti-TB therapy be combined with continued antitumor treatment. The patient continued to be treated with pembrolizumab. After 14 cycles, the lesion shrunk by 79%, there was no recurrence of infection, and there were no intolerable adverse reactions.

Conclusion: We have observed that in cases of lung cancer complicated with infection, opportunistic pathogen infection recurrence can be overcome, and immunotherapy is most beneficial when TB doctors and oncologists cooperate to closely observe dynamic changes in and lung cancer. Treatment should be maintained with low dosage anti-TB drugs after general anti-TB chemotherapy for 1 year; this may prevent opportunistic pathogen infection recurrence during immunotherapy.
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http://dx.doi.org/10.12998/wjcc.v10.i12.3801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100737PMC
April 2022
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