Publications by authors named "Peipei Li"

166 Publications

Epigenomic analysis of Parkinson's disease neurons identifies Tet2 loss as neuroprotective.

Nat Neurosci 2020 10 17;23(10):1203-1214. Epub 2020 Aug 17.

Center for Neurodegenerative Science, Van Andel Institute, Grand Rapids, MI, USA.

Parkinson's disease (PD) pathogenesis may involve the epigenetic control of enhancers that modify neuronal functions. Here, we comprehensively examine DNA methylation at enhancers, genome-wide, in neurons of patients with PD and of control individuals. We find a widespread increase in cytosine modifications at enhancers in PD neurons, which is partly explained by elevated hydroxymethylation levels. In particular, patients with PD exhibit an epigenetic and transcriptional upregulation of TET2, a master-regulator of cytosine modification status. TET2 depletion in a neuronal cell model results in cytosine modification changes that are reciprocal to those observed in PD neurons. Moreover, Tet2 inactivation in mice fully prevents nigral dopaminergic neuronal loss induced by previous inflammation. Tet2 loss also attenuates transcriptional immune responses to an inflammatory trigger. Thus, widespread epigenetic dysregulation of enhancers in PD neurons may, in part, be mediated by increased TET2 expression. Decreased Tet2 activity is neuroprotective, in vivo, and may be a new therapeutic target for PD.
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http://dx.doi.org/10.1038/s41593-020-0690-yDOI Listing
October 2020

Risk Perception and Depression in Public Health Crises: Evidence from the COVID-19 Crisis in China.

Int J Environ Res Public Health 2020 08 7;17(16). Epub 2020 Aug 7.

College of Public Administration, Central China Normal University, Wuhan 430079, China.

Scant attention has been paid to how risk perceptions of public health crises may affect people's mental health. : The aims of this study are to (1) construct a conceptual framework for risk perception and depression of people in public health crises, (2) examine how the mental health of people in the crisis of Coronavirus Disease 2019 (COVID-19) is affected by risk perception and its associated factors, including distance perception of the crisis and support of prevention and control policies, and (3) propose policy recommendations on how to deal with psychological problems in the current COVID-19 crisis. : Online questionnaire survey was implemented. A total of 6373 people visited the questionnaire online, 1115 people completed the questionnaire, and the number of valid questionnaires was 1081. Structural equation modeling was employed for data analysis. : Risk perception and its associated factors significantly affect the mental health of people in public health crises. Specifically, (1) distance perception of public health crises is negatively associated with depression among people, (2) affective risk perception is positively associated with depression of people in public health crises, (3) cognitive risk perception is negatively associated with depression of people in public health crises, and (4) support of prevention and control policies is negatively associated with depression of people in public health crises. : The findings of this study suggest that risk perception plays an important role in affecting the mental health of people in a public health crisis. Therefore, health policies aiming to improve the psychological wellbeing of the people in a public health crisis should take risk perception into consideration.
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http://dx.doi.org/10.3390/ijerph17165728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460398PMC
August 2020

Tuning the Surface Structure of Polyamide Membranes Using Porous Carbon Nitride Nanoparticles for High-Performance Seawater Desalination.

Membranes (Basel) 2020 Jul 24;10(8). Epub 2020 Jul 24.

Advanced Membranes and Porous Materials Center, Division of Physical Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Enhancing the water flux while maintaining the high salt rejection of existing reverse osmosis membranes remains a considerable challenge. Herein, we report the use of a porous carbon nitride (CN) nanoparticle to potentially improve both the water flux and salt rejection of the state-of-the-art polyamide (PA) thin film composite (TFC) membranes. The organic-organic covalent bonds endowed CN with great compatibility with the PA layer, which positively influenced the customization of interfacial polymerization (IP). Benefitting from the positive effects of CN, a more hydrophilic, more crumpled thin film nanocomposite (TFN) membrane with a larger surface area, and an increased cross-linking degree of PA layer was achieved. Moreover, the uniform porous structure of the CN embedded in the "ridge" sections of the PA layer potentially provided additional water channels. All these factors combined provided unprecedented performance for seawater desalination among all the PA-TFC membranes reported thus far. The water permeance of the optimized TFN membrane is 2.1-folds higher than that of the pristine PA-TFC membrane, while the NaCl rejection increased to 99.5% from 98.0%. Our method provided a promising way to improve the performance of the state-of-art PA-TFC membranes in seawater desalination.
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http://dx.doi.org/10.3390/membranes10080163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466004PMC
July 2020

Impact of liver fibrosis score on prognosis after common therapies for intrahepatic cholangiocarcinoma: a propensity score matching analysis.

BMC Cancer 2020 Jun 15;20(1):556. Epub 2020 Jun 15.

Department of General Surgery, Xiamen Haicang Hospital, 89 Haiyu Road, Haicang District, Xiamen, Fujian, China.

Background: Liver fibrosis or cirrhosis is associated with the dismal prognosis of hepatocellular carcinoma (HCC), and it might also be involved in intrahepatic cholangiocarcinoma (ICC). The effect of hepatic fibrosis on the survival of ICC patients is still unclear. This study aims to explore whether liver fibrosis impacts the overall survival (OS) and disease-specific survival (DSS) of ICC patients.

Methods: Data of 729 eligible ICC patients receiving different therapies from the Surveillance, Epidemiology, and End Results database (2004-2015) were analyzed. Unmatched, propensity score-matched, and propensity score-weighted cohorts were used to investigate the relationships of different fibrosis scores (low fibrosis score vs. high fibrosis score) and survival. A Cox regression and Kaplan-Meier curves were used to explore the influence of fibrosis score on patients' survival. Stratified analyses based on treatment modality were conducted to compare the survival difference in ICC patients with different fibrosis scores.

Results: Before matching, the one-, three-, and five-year OS were 50.9, 28.0, and 16.1% in the low fibrosis score group (n = 465) and 39.3, 20.1, and 8.0% in the high fibrosis score group (n = 264) (P < 0.001), respectively. After propensity score matching, the one-, three-, and five-year OS were 45.0, 26.0, and 10.2% in the low fibrosis score group and 36.0, 8.1, and 2.3% in the high fibrosis score group (P = 0.008), respectively. The multivariate Cox regression results showed that a high fibrosis score was an independent risk factor of OS. Additionally, patients with high fibrosis scores achieved low DSS after matching (P = 0.032). The survival benefits of the low fibrosis score group were consistent across treatment cohorts.

Conclusions: High fibrosis scores were associated with poor clinical outcomes of ICC patients receiving different common therapies.
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http://dx.doi.org/10.1186/s12885-020-07051-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296657PMC
June 2020

Insights into catalysis and regulation of non-canonical ubiquitination and deubiquitination by bacterial deamidase effectors.

Nat Commun 2020 06 2;11(1):2751. Epub 2020 Jun 2.

CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

The bacterial effector MavC catalyzes non-canonical ubiquitination of host E2 enzyme UBE2N without engaging any of the conventional ubiquitination machinery, thereby abolishing UBE2N's function in forming K63-linked ubiquitin (Ub) chains and dampening NF-кB signaling. We now report the structures of MavC in complex with conjugated UBE2N~Ub and an inhibitor protein Lpg2149, as well as the structure of its ortholog, MvcA, bound to Lpg2149. Recognition of UBE2N and Ub depends on several unique features of MavC, which explains the inability of MvcA to catalyze ubiquitination. Unexpectedly, MavC and MvcA also possess deubiquitinase activity against MavC-mediated ubiquitination, highlighting MavC as a unique enzyme possessing deamidation, ubiquitination, and deubiquitination activities. Further, Lpg2149 directly binds and inhibits both MavC and MvcA by disrupting the interactions between enzymes and Ub. These results provide detailed insights into catalysis and regulation of MavC-type enzymes and the molecular mechanisms of this non-canonical ubiquitination machinery.
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http://dx.doi.org/10.1038/s41467-020-16587-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265302PMC
June 2020

Construction of a Baculovirus Derivative to Produce Linearized Antheraea pernyi (Lepidoptera: Saturniidae) Multicapsid Nucleopolyhedrovirus Genomic DNA.

J Insect Sci 2020 Jan;20(2)

Liaoning Ocean and Fisheries Science Research Institute, Liaoning Academy of Agricultural Sciences, Dalian, China.

In the Antheraea pernyi multicapsid nucleopolyhedrovirus (AnpeNPV)-based expression vector system, the frequency of homologous recombination events between wild-type AnpeNPV DNA and the transfer vector is low, resulting in a small amount of recombinant virus. Previous reports have indicated that linearized baculovirus DNA can increase the proportion of recombinant virus relative to the total progeny. To improve the recombination efficiency, we constructed a linearized derivative of AnpeNPV, referred to as AnpeNPVPhEGFP-AvrII, in which egfp flanked by AvrII restriction sites was located at the polyhedrin locus and driven by the polyhedrin promoter. Linear AnpeNPV DNA was obtained by the treatment of AnpeNPVPhEGFP-AvrII genomic DNA with AvrII endonuclease. The infectivity and recombinogenic activity between the linearized and circular viral DNA were evaluated by quantitative real-time polymerase chain reactions. We demonstrated that the linearized AnpeNPV DNA produced only small numbers of infectious budded viruses, accounting for approximately 4.5% of the budded virus production of wild-type AnpeNPV DNA in A. pernyi pupae. However, the linearized AnpeNPV DNA substantially increased recombinant virus production after cotransfection with an appropriate transfer vector; relative abundance of the recombinant virus was approximately 5.5-fold higher than that of the wild-type AnpeNPV DNA in A. pernyi pupae. The linearization of AnpeNPV DNA will facilitate the purification of recombinant viruses using the AnpeNPV-based expression vector system and the construction of an AnpeNPV-based bacmid system.
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http://dx.doi.org/10.1093/jisesa/ieaa011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136005PMC
January 2020

DEAD-box RNA Helicase 39 Promotes Invasiveness and Chemoresistance of ER-positive Breast Cancer.

J Cancer 2020 20;11(7):1846-1858. Epub 2020 Jan 20.

The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China 325027.

DDX39 is a DEAD-box RNA helicase that unwinds double-stranded RNA in an ATP-dependent manner. This study evaluated the prognostic and predictive significance of DDX39 in breast cancer (BC). The cellular proliferation, invasion, and drug cytotoxicity by DDX39 siRNA were evaluated in MCF7 (ER-positive) and MDA-MB-231 (ER-negative) cell lines. A total of 27 datasets (total 8110 accessible cases) with following-up information were collected from Asia, Europe, and North America to explore associations between DDX39 gene expression and clinical parameters of BC patients. Down-regulation of DDX39 by siRNA significantly reduce the cell growth and invasion ability in MCF7 cells, but only slightly in MDA-MB-231 cells. The DDX39 mRNA level was elevated in breast adenocarcinoma compared with normal breast tissue (p<0.01). Higher DDX39 level was significantly correlated with larger tumor size (p<0.01) and poorer tumor differentiation (p<0.01). The prognostic significance of DDX39 for BC was assessed by pooled-analysis and meta-analysis. Kaplan-Meier analysis demonstrated that increased DDX39 mRNA expression was associated with poor outcomes significantly in a dose-dependent manner in ER-positive BC. The prognostic performance of DDX39 mRNA was comparable to 21-gene, 70-gene, and wound-response gene signatures, and it was superior to the TNM stage. Lower DDX39 expression was associated with reduced relative risk death on ER-positive BC with chemotherapy or radiotherapy. Inhibition of DDX39 by siRNA could significantly enhance the sensitivity of MCF-7 to doxorubicin. DDX39 may be a potential novel prognostic and predictive biomarker for BC patients with ER-positive status.
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http://dx.doi.org/10.7150/jca.37247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052869PMC
January 2020

Hemispheric asymmetry in the human brain and in Parkinson's disease is linked to divergent epigenetic patterns in neurons.

Genome Biol 2020 03 9;21(1):61. Epub 2020 Mar 9.

Center for Neurodegenerative Science, Van Andel Institute, Grand Rapids, MI, 49503, USA.

Background: Hemispheric asymmetry in neuronal processes is a fundamental feature of the human brain and drives symptom lateralization in Parkinson's disease (PD), but its molecular determinants are unknown. Here, we identify divergent epigenetic patterns involved in hemispheric asymmetry by profiling DNA methylation in isolated prefrontal cortex neurons from control and PD brain hemispheres. DNA methylation is fine-mapped at enhancers and promoters, genome-wide, by targeted bisulfite sequencing in two independent sample cohorts.

Results: We find that neurons of the human prefrontal cortex exhibit hemispheric differences in DNA methylation. Hemispheric asymmetry in neuronal DNA methylation patterns is largely mediated by differential CpH methylation, and chromatin conformation analysis finds that it targets thousands of genes. With aging, there is a loss of hemispheric asymmetry in neuronal epigenomes, such that hemispheres epigenetically converge in late life. In neurons of PD patients, hemispheric asymmetry in DNA methylation is greater than in controls and involves many PD risk genes. Epigenetic, transcriptomic, and proteomic differences between PD hemispheres correspond to the lateralization of PD symptoms, with abnormalities being most prevalent in the hemisphere matched to side of symptom predominance. Hemispheric asymmetry and symptom lateralization in PD is linked to genes affecting neurodevelopment, immune activation, and synaptic transmission. PD patients with a long disease course have greater hemispheric asymmetry in neuronal epigenomes than those with a short disease course.

Conclusions: Hemispheric differences in DNA methylation patterns are prevalent in neurons and may affect the progression and symptoms of PD.
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http://dx.doi.org/10.1186/s13059-020-01960-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063821PMC
March 2020

Radiofrequency ablation triggers the migration of hepatocellular carcinoma cells by suppressing miR-148a-5p.

Biol Chem 2020 07;401(8):985-994

Department of Oncology, The Third People's Hospital of Zhengzhou, No. 136 Nanshuncheng Street, Guangcheng District, Zhengzhou 450000, Henan Province,China.

Increasing evidences suggest that insufficient radiofrequency ablation (IRFA) can paradoxically promote tumor invasion and metastatic processes, whereas the effects of moderate hyperthermia on cancer progression are not well illustrated. Our study found that IRFA can increase the in vitro migration, invasion, and epithelial-mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells via induction of Snail, a master regulator of EMT events. Among measured miRNAs, IRFA can decrease the expression of miR-148a-5p in HCC cells. Whereas overexpression of miR-148a-5p can reverse IRFA-induced migration of HCC cells and upregulation of Snail, mechanistically overexpression of miR-148a-5p can directly target and decrease the expression of protein kinase ATM (ataxia telangiectasia mutated), which can increase protein stability of Snail. Collectively, our data suggest that IRFA can regulate the miR-148a-5p/ATM/Snail axis to trigger migration of HCC cells.
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http://dx.doi.org/10.1515/hsz-2020-0130DOI Listing
July 2020

Facile Preparation of MnO Quantum Dots with Enhanced Fluorescence via Microenvironment Engineering with the Assistance of Some Reductive Biomolecules.

ACS Appl Mater Interfaces 2020 Apr 17;12(13):15919-15927. Epub 2020 Mar 17.

Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China.

MnO nanomaterials have aroused widespread attention because of their nanozyme activity, redox properties, good biocompatibility, and therapy-related activities. However, not many reports on self-luminescent MnO materials have been concerned to date, which greatly hampered their further development in various fields. In this paper, luminescent MnO quantum dots (MnO QDs) have been first prepared via a facile one-step ultrasonic method. With the assistance of bovine serum albumin (BSA) or cysteine (Cys), the synthesized MnO QDs (BSA-MnO QDs or Cys-MnO QDs) display strongly enhanced fluorescence (FL). The prepared BSA-MnO QDs with a particle size of about 1 to 2 nm show the maximum excitation and emission peaks at 320 and 410 nm with excellent salt stability, anti-photobleaching ability, and time stability. It is confirmed that BSA plays a dual function as the exfoliating agent to promote the exfoliation of bulk MnO nanosheets and as the capping agent to provide a friendly microenvironment for MnO QDs. Ag ions can destroy the microenvironment of BSA-MnO QDs owing to the in situ formation of Ag nanoparticles (Ag NPs) mediated by BSA on the surface of the QDs. Then, these Ag NPs can quench the FL intensity of the QDs by fluorescence resonance energy transfer. However, the FL strength of the BSA-MnO QDs is recovered after adding HO and NaHS since they may react with Ag NPs to produce Ag and AgS, which further confirmed the role of BSA. This work not only opens up a facile and universal avenue to synthesize luminescent MnO QDs with enhanced FL but also provides a possible sensing platform through tuning the microenvironment of the MnO QDs. The MnO QDs with outstanding performance may show great potential as fluorescent probes in the fields of biological imaging, optical sensing, drug delivery, and therapy.
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http://dx.doi.org/10.1021/acsami.0c00917DOI Listing
April 2020

Electrocatalysis of N to NH by HKUST-1 with High NH Yield.

Chem Asian J 2020 Apr 25;15(8):1272-1276. Epub 2020 Feb 25.

Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education) College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.

Electrolytic ammonia synthesis from nitrogen at ambient conditions is appearing as a promising alternative to the Haber-Bosch process which is consuming high energy and emitting CO . Here, a typical MOF material, HKUST-1 (Cu-BTC, BTC=benzene-1,3,5-tricarboxylate), was selected as an electrocatalyst for the reaction of converting N to NH under ambient conditions. At -0.75 V vs. reversible hydrogen electrode, it achieves excellent catalytic performance in the electrochemical synthesis of ammonia with high NH yield (46.63 μg h  mg   or 4.66 μg h  cm ) and good Faraday efficiency (2.45%). It is indicated that the good performance of the HKUST-1 catalyst may originate from the formation of Cu(I). In addition, the catalyst also has good selectivity for N to NH .
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http://dx.doi.org/10.1002/asia.201901714DOI Listing
April 2020

The efficacy of parecoxib for pain control after hysterectomy: a meta-analysis of randomized controlled studies.

J Matern Fetal Neonatal Med 2019 Dec 6:1-8. Epub 2019 Dec 6.

Department of Obstetrics and Gynecology, Wenzhou People's Hospital, Wenzhou, China.

The efficacy of parecoxib for pain control after hysterectomy remains controversial. We conduct a systematic review and meta-analysis to explore the influence of parecoxib versus placebo on pain intensity after hysterectomy. We search PubMed, Embase, Web of Science, EBSCO, and Cochrane Library databases through March 2019 for randomized controlled trials (RCTs) assessing the effect of parecoxib versus placebo on pain intensity after hysterectomy. This meta-analysis is performed using the random-effect model. Six RCTs are included in the meta-analysis. Overall, compared with control group after hysterectomy, parecoxib treatment is associated with substantially reduced pain scores in 4-6 h at rest (MD = -0.98; 95%CI = -1.14 to -0.81;  < .00001), pain scores in 12 h at rest (MD = -0.70; 95%CI = -0.77 to -0.63;  < .00001), pain scores in 12 h on sitting up (MD = -0.90; 95%CI = -1.03 to -0.77;  < .00001), pain scores in 24 h on sitting up (MD = -1.19; 95%CI = -1.94 to -0.44;  = .002), dose of analgesic need in parecoxib group is notably lower than that in control group (std. MD = -2.54; 95%CI = -3.97 to -1.10;  = .0005), but shows no obvious effect on pain scores in 24 h at rest (MD = -0.40; 95%CI = -1.47-0.67;  = .47), pain scores in 4-6 h on sitting up (MD = -0.54; 95%CI = -2.50-1.42;  = .59), first time to analgesic requirement between two groups (std. MD = -0.10; 95%CI = -0.47-0.26;  = .57), nausea or vomiting (RR = 0.92; 95%CI = 0.59-1.43;  = .70), and adverse events (RR = 0.86; 95%CI = 0.64-1.17;  = .34). Parecoxib treatment provides additional benefits for pain control after hysterectomy.
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http://dx.doi.org/10.1080/14767058.2019.1685972DOI Listing
December 2019

Resveratrol improves left ventricular remodeling in chronic kidney disease via Sirt1-mediated regulation of FoxO1 activity and MnSOD expression.

Biofactors 2020 Jan 5;46(1):168-179. Epub 2019 Nov 5.

Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.

Left ventricular remodeling commonly complicates end-stage renal disease following chronic kidney disease (CKD). This study investigated the therapeutic efficacy of resveratrol (RSV), a polyphenolic compound, on left ventricular remodeling in subtotal nephrectomy rats and sought to uncover the underlying molecular mechanisms. Subtotal nephrectomy caused renal dysfunction, such as gradual increases in serum creatinine and blood urea nitrogen, glomerular sclerosis, and tubulointerstitial fibrosis. In addition, subtotal nephrectomy also resulted in significant increases in myocyte cross-sectional area, interstitial and perivascular fibrosis, and left ventricular dilatation. All these detrimental effects were alleviated in the presence of RSV. Mechanistically, RSV treatment led to the upregulation of manganese-containing superoxide dismutase (MnSOD) in the heart. Coimmunoprecipitation studies showed that silent information regulator 1 (Sirt1) bound forkhead box protein O1 (FoxO1) and thus reduced acetylated FoxO1. RSV strengthened this interaction between Sirt1 and FoxO1. Loss of one allele of Sirt1 aggravated renal damage, myocyte hypertrophy, and interstitial fibrosis in nephrectomized mice. Taken together, our data show that Sirt1 is an important mediator for the protective roles of RSV on renal and heart damage in CKD rodent model, and FoxO1 and MnSOD are likely downstream targets of Sirt1. Therefore, Sirt1 might be a potential therapeutic target for the treatment of left ventricular remodeling caused by CKD.
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http://dx.doi.org/10.1002/biof.1584DOI Listing
January 2020

Listeria-based hepatocellular carcinoma vaccine facilitates anti-PD-1 therapy by regulating macrophage polarization.

Oncogene 2020 02 28;39(7):1429-1444. Epub 2019 Oct 28.

Department of Immunology, Key Laboratory of Immune Microenvironment and Disease, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.

Recently, patients with advanced cancers have been benefited greatly from immune checkpoint blockade immunotherapy. However, immune checkpoint blockade is still suboptimal in HCC treatment and more immune modifications are needed to achieve an efficient therapeutic goal. Here, we investigated the combined administration of a Listeria-based HCC vaccine, Lmdd-MPFG, and the anti-PD-1 immune checkpoint blockade antibody. We found that Lmdd-MPFG promoted the expression of PD-L1 in HCC cells but resensitized the tumor local T cell to respond to the anti-PD-1 immunotherapy. Mechanistically, the Lmdd-MPFG vaccine activates the NF-κB pathway in the tumor-associated macrophages (TAMs) through the TLR2 and MyD88 pathway, and recruits p62 to activate the autophagy pathway. The overall effect is skewing the TAMs from M2-polarized TAMs into the M1-polarized TAMs. Most importantly, it skewed the cytokine profiles into antitumor one in the tumor microenvironment (TME). This change restores the T-cell reactivity to the anti-PD-1 blockade. Our results suggested that Lmdd-MPFG combined with PD-1 blockade exerted synergistic antitumor effects through modifying TAMs in the TME and removing T-cell inhibitory signals, thereby providing a new potential strategy for HCC treatment.
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http://dx.doi.org/10.1038/s41388-019-1072-3DOI Listing
February 2020

A polyether amine modified metal organic framework enhanced the CO adsorption capacity of room temperature porous liquids.

Chem Commun (Camb) 2019 Oct;55(87):13179-13182

State Key Laboratory of Marine Resources Utilization in South China Sea, Hainan University, Haikou, Hainan 570228, P. R. China.

Based on both the compatibility principle of similar polymeric structures and the steric interaction, a polyether amine (D2000) modified MOF (UIO-66) was dispersed into an ionic liquid with a polyether structure to form a new porous liquid (called the UIO-66-liquid) at room temperature. This unique UIO-66-liquid showed an outstanding CO2 uptake capacity among the existing porous liquids.
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http://dx.doi.org/10.1039/c9cc07243hDOI Listing
October 2019

Nuclear localization of Desmoplakin and its involvement in telomere maintenance.

Int J Biol Sci 2019 24;15(11):2350-2362. Epub 2019 Aug 24.

Cancer Center, Faculty of Health Sciences, University of Macau, Macau, SAR of China.

The interaction between genomic DNA and protein fundamentally determines the activity and the function of DNA elements. Capturing the protein complex and identifying the proteins associated with a specific DNA locus is difficult. Herein, we employed CRISPR, the well-known gene-targeting tool in combination with the proximity-dependent labeling tool BioID to capture a specific genome locus associated proteins and to uncover the novel functions of these proteins. By applying this research tool on telomeres, we identified DSP, out of many others, as a convincing telomere binding protein validated by both biochemical and cell-biological approaches. We also provide evidence to demonstrate that the C-terminal domain of DSP is required for its binding to telomere after translocating to the nucleus mediated by NLS sequence of DSP. In addition, we found that the telomere binding of DSP is telomere length dependent as hTERT inhibition or knockdown caused a decrease of telomere length and diminished DSP binding to the telomere. Knockdown of TRF2 also negatively influenced DSP binding to the telomere. Functionally, loss of DSP resulted in the shortened telomere DNA and induced the DNA damage response and cell apoptosis. In conclusion, our studies identified DSP as a novel potential telomere binding protein and highlighted its role in protecting against telomere DNA damage and resultant cell apoptosis.
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http://dx.doi.org/10.7150/ijbs.34450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775319PMC
May 2020

Multicolor Aptasensor Based on DNA-Induced Au-Ag Nanorods for Simultaneous and Visual Detection of Inorganic and Organic Mercury.

ACS Omega 2019 Sep 6;4(12):15112-15119. Epub 2019 Sep 6.

Key Laboratory for Analytical Science of Food Safety and Biology of MOE, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.

Compared to inorganic mercury (Hg), methyl-mercury (CHHg) and ethyl-mercury (CHHg) (organic mercury) not only have a much stronger toxicity but also are more easily accumulated by marine organisms to produce bioamplification. Therefore, the simultaneously onsite detection of Hg and organic mercury is of great significance to ensure the safety of seafood, and it is also a hard challenge. We designed a T-rich aptamer, H, for specifically recognizing Hg and organic mercury and developed a multicolor aptasensor for simultaneous discrimination and detection of Hg and organic mercury with only bare-eye observation using H as a recognition probe and gold nanorods (AuNRs) as a signal. In the presence of Hg and Ag, Hg preferentially and specifically bind with H immobilized on AuNRs surface and induce the formation of a monolayer Ag/Hg amalgam on the AuNRs surface after reduction, resulting in a change in color from orange to faint purple and a corresponding shift in the absorption peak from 820 to 730 nm in the solution. However, in the presence of CHHg or CHHg and Ag, CHHg or CHHg preferentially bind with H immobilized on the AuNRs surface and induce the formation of a monolayer Ag on the AuNRs surface after reduction, which results in the change in color from orange to atrovirens and the corresponding shift in the absorption peak shift from 820 to 670 nm in the solution. Thus, the inorganic and organic mercury (total of CHHg and CHHg) can be specifically discriminated and detected by only bare-eye observation. The method can be used to simultaneously detect inorganic and organic mercury in seawater by the bare-eye observation with a visual detection limit of 2.0 ppm for Hg and 10.0 ppm for organic mercury. The success of this study is a useful enlightenment to develop an instrument-free method for an onsite detection of trace inorganic and organic mercury in environment by a bare-eye observation, although the sensitivity of the method is relatively low.
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http://dx.doi.org/10.1021/acsomega.9b01994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751705PMC
September 2019

[Folic acid relieves rat cardiomyocyte injury induced by homocystein through DNA methylation].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2019 Jul;35(7):625-630

Department of Internal Medicine, Heart Centre, General Hospital of Ningxia Medical University, Yinchuan 750004, China.

Objective To investigate the effect of homocysteine (Hcy) on the cardiomyocytes cultured in vitro, and to analyze the role of folic acid in DNA methylation to explore the protective effect and mechanism of folic acid during Hcy exposure of H9C2 cardiomyocytes. Methods H9C2 cells were treated with Hcy at different concentrations (0, 0.5, 1, 2) mmol/L for 24 hours. Cell viability was tested by CCK-8 assay. The apoptosis was detected by flow cytometry. H9C2 cells were divided into 2 mmol/L Hcy group, 0.1 mmol/L folic acid combined with 2 mmol/L Hcy group, 0.1 mmol/L folic acid group and DMSO control group. The above corresponding treatment lasted 24 hours. Then we detected the cell viability and apoptosis. The total DNA methylation level was determined by MethylFlash ELISA kit. DNMT1, DNMT3a, DNMT3b mRNA and protein expression were detected by real-time quantitative PCR and Western blot analysis. Results The number of H9C2 cells treated with different concentrations of Hcy for 24 hours decreased with the increase of Hcy concentration. Compared with the control group, the activity and apoptosis of the cells in the 2 mmol/L Hcy treatment group were reduced, and the number of cells in the folic acid combined with Hcy treatment group was significantly higher than that in the Hcy treatment group. Compared with the other groups, the total apoptosis rate of Hcy treatment group increased, methylation level decreased significantly, and the level of DNA methylation increased in the folic acid combined with Hcy treatment group. The level of DNMT1 mRNA significantly increased only in the folic acid treatment group, and the levels of DNMT1, DNMT3a and DNMT3b were not significantly changed. Conclusion Folic acid can relieve the damage of Hcy to myocardial cells by DNA methylation.
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July 2019

Research progress on the removal of hazardous perfluorochemicals: A review.

J Environ Manage 2019 Nov 6;250:109488. Epub 2019 Sep 6.

Hunan International Scientific and Technological Cooperation Base of Agricultural Typical Pollution Remediation and Wetland Protection, College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China. Electronic address:

Perfluorinated substances are global and ubiquitous pollutants. The persistent organic pollution of perfluorochemicals (PFCs) have drawn attentions worldwide. In view of the current need for sustainable development, many researchers began to study the remediation techniques for PFCs. Due to its unique hydrophobic and oil-phobic characteristics, the requirements for the PFCs removal process are different, so that their remediation techniques are still under continuous exploration. Hence, this review summarized the removal behaviors of various PFCs on different materials which supply a good foundation for future investigations in this field. It is evident from previous literature that every remediation techniques for PFCs has its own advantages. Among various currently evaluated removal methods, adsorption seems to be one of the most commonly used and recognized techniques for PFCs pollution control. Other innovative and promising techniques, such as physical and/or chemical methods, have also been tested for their effectiveness in removing perfluorinated compounds.
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http://dx.doi.org/10.1016/j.jenvman.2019.109488DOI Listing
November 2019

Sulfonated Sub-Nanochannels in a Robust MOF Membrane: Harvesting Salinity Gradient Power.

ACS Appl Mater Interfaces 2019 Sep 11;11(38):35496-35500. Epub 2019 Sep 11.

State Key Laboratory of Silicon Materials, Department of Materials Science and Engineering , Zhejiang University , Zheda Road 38 , Hangzhou 310027 , China.

We developed a robust, crack-free ultrathin zeolite- imidazole framework (ZIF-8) membrane in-built with a sulfonate-ion-containing polymer (ZIF) via a vapor-assisted in situ conversion process. The sulfonated sub-nanochannels of the ZIF membrane afforded a rapid and selective transport of Li over counteranions and other alkali ions due to electrostatic repulsion and optimal transport kinetics of cation-sulfonate ion pairs. A salinity gradient power generator (SGPG) was built by using the ZIF membrane as a cell separator coupled with a pair of Ag/AgCl porous membrane electrodes. At a salinity gradient of 10, such a power generator presented a significantly decreased internal resistance (25.6 Ω), three-order of magnitude lower than that reported previously, and an output power as high as 9.03 μW.
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http://dx.doi.org/10.1021/acsami.9b13617DOI Listing
September 2019

Application of high-resolution CT images information in complicated infection of lung tumors.

J Infect Public Health 2021 Mar 23;14(3):418-422. Epub 2019 Aug 23.

Department of CT, Hengshui People's Hospital, Hengshui, 053000, Hebei, China. Electronic address:

To explore the quality of high-resolution CT images information in the evaluation of pulmonary nodule interface and internal structure of nodules in lung tissue, as well as the value of early diagnosis of lung cancer associated with infection, high-resolution CT images were used as the research object. Through the analysis of the computerized detection and diagnosis (Computer-Aided Diagnosis (CAD)) of lung cancer, the high-resolution CT was further explored in the process of clinical imaging doctors in the diagnosis of lung cancer, and more conditions were created for the application of medical image processing in the early diagnosis of lung cancer. The research results show that CAD can automatically and accurately complete the automatic segmentation of the lung region in the CT image by applying the automatic segmentation algorithm for a series of processing and analysis of the CT image, that is, generating high-resolution CT images. It can enhance the pulmonary nodules in CT images and improve the accuracy of lung nodule detection, which is of great value in the diagnosis of early lung cancer. CAD diagnosis of lung lesions based on high-resolution CT images is studied, which can provide reference for imaging physicians to diagnose early lung cancer. However, in the automatic identification of benign and malignant lesions in the lungs, it is necessary to further improve the analysis function of similar nodules, which will be an important step for humans in the diagnosis and treatment of diseases.
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http://dx.doi.org/10.1016/j.jiph.2019.08.001DOI Listing
March 2021

Simultaneous Recovery of Metal Ions and Electricity Harvesting via [email protected] Membrane.

ACS Appl Mater Interfaces 2019 Sep 6;11(37):34039-34045. Epub 2019 Sep 6.

State Key Laboratory of Silicon Materials, School of Materials Science and Engineering , Zhejiang University , Hangzhou 310027 , China.

The spent lithium-ion batteries contain significant amounts of valuable metals such as lithium and cobalt. However, how to effectively recover these valuable metals and minimize environmental pollution simultaneously is still a challenge. In this work, a natural biopolymer K-Carrageenan is introduced into a stable metal-organic framework ZIF-8 to form a composite (KCZ) membrane for selectively separating Li from Co and simultaneously harvesting the concentration gradient energy efficiently. The prepared KCZ membrane shows an Li ionic conductivity of up to 1.70 × 10 S cm, 5 orders of magnitude higher than 1.1 × 10 S cm for pristine ZIF-8, with an Li flux of 0.342 mol m h and a selectivity of about 8.29 for Li over Co. Moreover, this asymmetric KCZ/anodic alumina oxide membrane exhibits a good output power of up to 3.54 μW when employed as a concentration-gradient energy-harvesting device during the separation process. Hence, the KCZ membrane shows great potential in application for advanced separation and simultaneous concentration gradient energy harvesting.
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http://dx.doi.org/10.1021/acsami.9b12501DOI Listing
September 2019

STAT6 phosphorylation upregulates microRNA-155 expression and subsequently enhances the pathogenesis of chronic lymphocytic leukemia.

Oncol Lett 2019 Jul 30;18(1):95-100. Epub 2019 Apr 30.

Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.

Chronic lymphocytic leukemia (CLL), a clonal expansion of CD5 B cells, is the most common form of adult leukemia; however, the molecular mechanisms underlying its pathogenesis remain undetermined. It has been previously suggested that numerous biological factors, including cytokines, may be involved in the proliferation of malignant cells. For example, interleukin (IL)-4, IL-2, interferon-γ and tumor necrosis factor serve roles as inhibitors of cellular apoptosis; whereas IL-5 and IL-10 are inducers of cellular apoptosis. In the present study, the results demonstrated that the phosphorylation and activation of signal transducer and activator of transcription 6 (STAT6) was induced by IL-4 in a time-dependent manner. Notably, the expression level of microRNA (miR)-155 was increased in MEC-1 cells following treatment with IL-4; however, this effect was attenuated following STAT6 knockdown via RNA interference. In addition, STAT6 knockdown promoted cell apoptosis, which was partly attenuated by treatment with IL-4. Inhibition of miR-155 expression significantly increased cell apoptosis despite the presence of IL-4. The results of the present study suggested that treatment with IL-4 enhanced the expression of miR-155, which regulated CLL cell survival via the enhanced phosphorylation of STAT6.
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http://dx.doi.org/10.3892/ol.2019.10294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540395PMC
July 2019

Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.

J Proteome Res 2019 08 5;18(8):2987-2998. Epub 2019 Jul 5.

Cancer Center, Faculty of Health Sciences , University of Macau , Macau , SAR of China.

Protein-protein interactions (PPIs) are indispensable for the dynamic assembly of multiprotein complexes that are central players of nearly all of the intracellular biological processes, such as signaling pathways, metabolic pathways, formation of intracellular organelles, establishment of cytoplasmic skeletons, etc. Numerous approaches have been invented to study PPIs both in vivo and in vitro, including the protein-fragment complementation assay (PCA), which is a widely applied technology to study PPIs and biomolecular interactions. PCA is a technology based on the expression of the bait and prey proteins in fusion with two complementary reporter protein fragments, respectively, that will reassemble when in close proximity. The reporter protein can be the enzymes or fluorescent proteins. Recovery of the enzymatic activity or fluorescent signal can be the indicator of PPI between the bait and prey proteins. Significant effort has been invested in developing many derivatives of PCA, along with various applications, in order to address specific questions. Therefore, a prompt review of these applications is important. In this review, we will categorize these applications according to the scenarios that the PCAs were applied and expect to provide a reference guideline for the future selection of PCA methods in solving a specific problem.
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http://dx.doi.org/10.1021/acs.jproteome.9b00154DOI Listing
August 2019

A dual (colorimetric and fluorometric) detection scheme for glutathione and silver (I) based on the oxidase mimicking activity of MnO nanosheets.

Mikrochim Acta 2019 07 3;186(8):498. Epub 2019 Jul 3.

Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People's Republic of China.

A fluorimetric and colorimetric method is described for the determination of glutathione (GSH) and silver (I). It is based on the use of MnO nanosheets that were prepared by solution mixing and exfoliation. They display oxidase-mimicking activity and can catalyze the oxidation of o-phenylenediamine (OPD) to form yellow 2,3-diaminophenazine (DAP) with an absorption maximum at 410 nm. DAP also has a yellow fluorescence (with a peak at 560 nm). The MnO nanosheets can be rapidly reduced to Mn by GSH. This reduces the efficiency of the oxidase mimic MnO and causes a decrease in fluorescence and absorbance intensity. However, on addition of Ag, a complex is formed with GSH. It prevents the destruction of MnO nanosheets so that the enzyme mimicking activity is retained. A dual-method for the determination of GSH and Ag(I) was developed. It has excellent sensitivity for GSH with lower detection limits of 62 nM (fluorimetric) and 0.94 μM (colorimetric). The respective data for Ag(I) are 70 nM and 1.15 μM. The assay was successfully applied to the determination of GSH and Ag(I) in spiked serum samples. Graphical abstract Schematic presentation of a method for colorimetric and fluorometric determination of glutathione (GSH) and silver(I). MnO nanosheets are reduced to Mn(II) by GSH. This reduces the enzyme-mimicking activity of MnO nanosheets and causes a decrease in fluorescence and absorbance. On addition of Ag(I), the enzyme-like activity is increasingly retained. A decrease in fluorescence and absorbance is not observed any longer.
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http://dx.doi.org/10.1007/s00604-019-3613-4DOI Listing
July 2019

Dissociable cognitive patterns related to depression and anxiety in multiple sclerosis.

Mult Scler 2020 09 24;26(10):1247-1255. Epub 2019 Jun 24.

Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USA/Corinne Goldsmith Dickinson Center for Multiple Sclerosis, Mount Sinai Hospital, New York, NY, USA.

Background: Individuals with multiple sclerosis (MS) frequently present with depression and anxiety, as well as cognitive impairment, challenging clinicians to disentangle interrelationships among these symptoms.

Objective: To identify cognitive functions associated with anxiety and depression in MS.

Methods: Mood and cognition were measured in 185 recently diagnosed patients (Reserve Against Disability in Early Multiple Sclerosis (RADIEMS) cohort), and an independent validation sample (MEM CONNECT cohort,  = 70). Partial correlations evaluated relationships of cognition to anxiety and depression controlling for age, sex, education, and premorbid verbal intelligence.

Results: In RADIEMS cohort, lower anxiety was associated with better nonverbal memory ( = -0.220,  = 0.003) and lower depression to better attention/processing speed ( = -0.241,  = 0.001). Consistently, in MEM CONNECT cohort, lower anxiety was associated with better nonverbal memory ( = -0.271,  = 0.028) and lower depression to better attention/processing speed ( = -0.367,  = 0.002). Relationships were unchanged after controlling for T2 lesion volume and fatigue.

Conclusion: Consistent mood-cognition relationships were identified in two independent cohorts of MS patients, suggesting that cognitive correlates of anxiety and depression are separable. This dissociation may support more precise models to inform treatment development. Treatment of mood symptoms may mitigate effects on cognition and/or treatment of cognition may mitigate effects on mood.
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http://dx.doi.org/10.1177/1352458519860319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928451PMC
September 2020

Colorimetric detection of ascorbic acid and alkaline phosphatase activity based on the novel oxidase mimetic of Fe-Co bimetallic alloy encapsulated porous carbon nanocages.

Talanta 2019 Sep 7;202:354-361. Epub 2019 May 7.

Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.

A novel catalyst of FeCo nanoparticles (FeCo NPs) incorporated porous nanocages (FeCo [email protected]) was first synthesized by encapsulating of FeCo alloy into ZIF-8 and further carbonation of the composite. The FeCo [email protected] displays enhanced intrinsic oxidase-like activity compared to the individual FeCo NPs and porous nanocages (PNC). The FeCo [email protected] can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) without HO, producing a blue color with a maximum absorption peak at 652 nm. The catalytic mechanism was investigated and it found that the intermediate (O) produced from the catalytic process in the system of TMB-O-FeCo [email protected] can accelerate the oxidation of TMB to oxTMB. However, ascorbic acid (AA) can reduce the oxTMB and result in a conspicuous blue color fading. Therefore, a novel colorimetric platform was constructed to quantify AA with the linear range of 0.5-28 μM and detection limit of 0.38 μM (at 3σ/m). Owing to the alkaline phosphatase (ALP) can catalyze the hydrolysis of AA 2-phosphate (AAP) into AA, ALP can also be quantified by the above method. And the linear range for ALP is 0.6-10 U L and the limit of detection is 0.49 U L. The FeCo [email protected] also shows excellent stability and reproducibility. This study provides a new alternative oxidase mimetic on the basis of easily obtained metal-organic frameworks derivatives to replace the expensive natural enzymes and noble metal based nanoenzymes, which will show great potential in biological assays.
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http://dx.doi.org/10.1016/j.talanta.2019.05.034DOI Listing
September 2019

Bioactive Exopolysaccharides Reveal Infected by the Fungus Could Have a Functional Use.

Molecules 2019 May 29;24(11). Epub 2019 May 29.

College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, China.

is an important Chinese commercial crop. can display abnormal leaves due to infection by the parasitic fungus . was isolated from infected leaves and used in fermentation, and exopolysaccharides EP0-1 and EP0.5-1 were purified from the fermentation broth. EP0-1 was an alkaline polysaccharide consisting mainly of the linkages α-d-Manp(1→, →2)-α-d-Manp(1→ and →6)-α-d-Manp(1→, →3)-α-d-Glcp(1→ and→4)-α-d-Glcp(1→, terminal β-d-Galf, (1→5)-β-d-Galf, and terminal β-D-GlcN(1→. EP0.5-1 was an acidic galactofuranose-containing polysaccharide. It contained the linkages of α-d-Manp(1→, →2)-α-d-Manp(1→, →6)-α-d-Manp(1→,→2, 6)-α-d-Manp(1→, →4)-α-d-Glcp(1→, and →4)-α-d-GlcUA(1→. Galactofuranose linkages were composed of terminal β-d-Galf, (1→6)-β-d-Galf and (1→2)-β-d-Galf. exopolysaccharides displayed significant immunoregulatory activity by activating macrophages. This research indicates that infected leaves from including the exopolysaccharides produced by the parasitic fungus by are worth further investigation as a functional product.
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http://dx.doi.org/10.3390/molecules24112048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600561PMC
May 2019

Epigenetic dysregulation of enhancers in neurons is associated with Alzheimer's disease pathology and cognitive symptoms.

Nat Commun 2019 05 21;10(1):2246. Epub 2019 May 21.

Center for Neurodegenerative Science, Van Andel Research Institute, Grand Rapids, MI, 49503, USA.

Epigenetic control of enhancers alters neuronal functions and may be involved in Alzheimer's disease (AD). Here, we identify enhancers in neurons contributing to AD by comprehensive fine-mapping of DNA methylation at enhancers, genome-wide. We examine 1.2 million CpG and CpH sites in enhancers in prefrontal cortex neurons of individuals with no/mild, moderate, and severe AD pathology (n = 101). We identify 1224 differentially methylated enhancer regions; most of which are hypomethylated at CpH sites in AD neurons. CpH methylation losses occur in normal aging neurons, but are accelerated in AD. Integration of epigenetic and transcriptomic data demonstrates a pro-apoptotic reactivation of the cell cycle in post-mitotic AD neurons. Furthermore, AD neurons have a large cluster of significantly hypomethylated enhancers in the DSCAML1 gene that targets BACE1. Hypomethylation of these enhancers in AD is associated with an upregulation of BACE1 transcripts and an increase in amyloid plaques, neurofibrillary tangles, and cognitive decline.
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http://dx.doi.org/10.1038/s41467-019-10101-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529540PMC
May 2019

Differential methylation of enhancer at IGF2 is associated with abnormal dopamine synthesis in major psychosis.

Nat Commun 2019 05 3;10(1):2046. Epub 2019 May 3.

Center for Neurodegenerative Science, Van Andel Research Institute, Grand Rapids, 49503, MI, USA.

Impaired neuronal processes, including dopamine imbalance, are central to the pathogenesis of major psychosis, but the molecular origins are unclear. Here we perform a multi-omics study of neurons isolated from the prefrontal cortex in schizophrenia and bipolar disorder (n = 55 cases and 27 controls). DNA methylation, transcriptomic, and genetic-epigenetic interactions in major psychosis converged on pathways of neurodevelopment, synaptic activity, and immune functions. We observe prominent hypomethylation of an enhancer within the insulin-like growth factor 2 (IGF2) gene in major psychosis neurons. Chromatin conformation analysis revealed that this enhancer targets the nearby tyrosine hydroxylase (TH) gene responsible for dopamine synthesis. In patients, we find hypomethylation of the IGF2 enhancer is associated with increased TH protein levels. In mice, Igf2 enhancer deletion disrupts the levels of TH protein and striatal dopamine, and induces transcriptional and proteomic abnormalities affecting neuronal structure and signaling. Our data suggests that epigenetic activation of the enhancer at IGF2 may enhance dopamine synthesis associated with major psychosis.
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http://dx.doi.org/10.1038/s41467-019-09786-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499808PMC
May 2019
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