Publications by authors named "Peili Gao"

4 Publications

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An evaluation of the efficacy of percutaneous reduction and screw fixation without bone grafting in Sanders Type-II and Type-III displaced intra-articular calcaneal fractures.

BMC Musculoskelet Disord 2022 Jun 10;23(1):562. Epub 2022 Jun 10.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Rd, Chongqing, 400016, China.

Background: The aim of this retrospective monocentric study was to investigate the clinical efficacy of percutaneous reduction and screw fixation without bone grafting in Sanders Type-II and Type-III displaced intra-articular calcaneal fractures (DIACFs).

Methods: The medical records of calcaneal fractures patients who were admitted to our department from January 2018 to January 2020 were retrospectively reviewed, and those meeting the inclusion criteria were fnally included for analysis. All patients were treated with percutaneous reduction and screw fixation, and no patients received bone grafting. The radiologic parameters evaluated included the BÖhler angle and the calcaneal height. In addition, the American Orthopaedic Foot and Ankle Society (AOFAS) hindfoot scores, Maryland Foot Score (MFS), and visual analog scale (VAS) score were determined.

Results: Thirty-eight patients with Sanders Type-II and Type-III DIACFs were finally included, including 30 males and 8 females aged 21 to 61 years [(42.6 ± 9.6) years]. According to the Essex-Lopresti classification, 27 of the fractures were the tongue type, and 11 were the joint compression type. According to the Sanders classification, 27 of the fractures were type II, and 11 were type III. Immediately postoperatively, the calcaneal height had recovered to 39.8 ± 2.1 mm, the BÖhler angle had recovered from 4.2° ± 13.6° preoperatively to 27.2° ± 3.4° (P = 0.000). All patients were followed up for 18-42 months [(25.2 ± 9.5) months]. All fractures healed. No differences were found in the outcome measures six-months postoperatively (BÖhler angle, p = 0.24; calcaneal height, p = 0.82) or at final follow-up (BÖhler angle, p = 0.33; calcaneal height, p = 0.28) compared to the immediately postoperative values. At the final follow-up, the AOFAS score was 91.7 ± 7.4 points, with an excellent and good rate of 92.1%; the MFS was 90.3 ± 7.8 points, with an excellent and good rate of 92.1%; and the VAS score was 2.2 ± 1.5 points. None of the patients had incision complications, and one patient developed traumatic arthritis.

Conclusion: Percutaneous reduction and screw fixation without bone grafting in Sanders Type-II and Type-III DIACFs can achieve good recovery and maintenance of the BÖhler angle and calcaneal height. Moreover, it has the advantage of a low complication rate.
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http://dx.doi.org/10.1186/s12891-022-05515-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188138PMC
June 2022

A facile microwave-assisted synthesis of highly crystalline red carbon dots by adjusting the reaction solvent for white light-emitting diodes.

Nanotechnology 2020 May 30;31(21):215704. Epub 2020 Jan 30.

Institute of Semiconductor Science and Technology, Guangdong Engineering Technology Research Center of Low Carbon and Advanced Energy Materials, South China Normal University, Guangzhou, 510631, People's Republic of China.

Microwave approach has been widely used for the synthesis of fluorescent carbon dots (CDs) due to its advantages of short reaction time and homogeneous heating. Currently, it is still difficult to synthesize red light-emitting CDs (R-CDs) via the microwave-assisted method at atmospheric pressure. Herein, we report a solvent-controlled synthesis of R-CDs using domestic microwave. As a result, for an ammonium citrate tribasic, formamide and glycerol blend reaction solvent, the R-CDs with emission peak of 622 nm were achieved and exhibited a photoluminescence (PL) quantum yield of 37.4% and excitation-independent PL emission spectrum. During the formation process of R-CDs, glycerol and formamide not only promote the carbonization of ammonium citrate tribasic, but can also enhance the crystalline nature of CDs. Finally, the warm white light-emitting diode (WLED) with a high colour rendering index (CRI) of 90.9 and Commission Internationale de L'Eclairage (CIE) coordinates of (0.344, 0.333) was realized by combining conventional yellow YAG:Ce phosphor and R-CDs/mesoporous silica (MPS) composites with a blue chip. This result demonstrates a low-cost R-CD synthetic method for potential substitution for red phosphor materials in solid-state lighting applications.
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http://dx.doi.org/10.1088/1361-6528/ab71b6DOI Listing
May 2020

The DmtA methyltransferase contributes to Aspergillus flavus conidiation, sclerotial production, aflatoxin biosynthesis and virulence.

Sci Rep 2016 Mar 16;6:23259. Epub 2016 Mar 16.

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

DNA methylation is essential for epigenetic regulation of gene transcription and development in many animals, plants and fungi. We investigated whether DNA methylation plays a role in the development and secondary metabolism of Aspergillus flavus, identified the DmtA methyltransferase from A. flavus, and produced a dmtA knock-out mutant by replacing the dmtA coding sequence with the pyrG selectable marker. The A. flavus dmtA null mutant lines produced white fluffy mycelium in liquid medium, and displayed a slightly flavescent conidial pigmentation compared with the normal yellow of the wild-type strain when grown on agar. The ΔdmtA lines exhibited decreased conidiation and aflatoxin (AF) biosynthesis, compared with the wild-type line, suggesting that the DmtA knock-out affected the transcriptional level of genes in the AF cluster. In particular, sclerotia development and host colonization were altered in the dmtA null mutants. Green fluorescent protein tagging at the C-terminus of DmtA showed that DmtA localized to the nucleus and cytoplasm. DNA methylation content measurements in the dmtA mutants revealed no widespread DNA methylation in the mutants or wild-type lines. Thus, our findings suggest that DmtA, apart from being a C-5 cytosine methyltransferase in A. flavus, contributes to asexual development, aflatoxin biosynthesis, sclerotial production and virulence.
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http://dx.doi.org/10.1038/srep23259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793245PMC
March 2016

sRNA profiling in Aspergillus flavus reveals differentially expressed miRNA-like RNAs response to water activity and temperature.

Fungal Genet Biol 2015 Aug 24;81:113-9. Epub 2015 Mar 24.

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:

Small non-coding RNA (sRNA) in various organisms remains a mysterious subject. Although microRNAs (miRNAs) have been intensively investigated in plants and animals, the study of miRNAs in fungi has been limited. Only microRNA-like RNAs (milRNAs) have been reported in several filamentous fungi. In this study, Illumina deep sequencing was performed to characterize the sRNA in Aspergillus flavus and to evaluate their responses to water activity and temperature. Global expression analysis showed an extensively differential expression of sRNA loci in A. flavus under different temperature or water activities. In addition, a total of 135 milRNAs were identified in A. flavus. The milRNA profiles obtained in deep sequencing were further validated by RT-qPCR assay. The presence and differential expression of milRNAs under different temperature or water activities in A. flavus imply that milRNAs might play important roles in the mycotoxin biosynthesis and mycelium growth in fungi A. flavus.
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http://dx.doi.org/10.1016/j.fgb.2015.03.004DOI Listing
August 2015
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