Publications by authors named "Pei Wen"

16 Publications

  • Page 1 of 1

Supporting People With Stroke to Return to Work in Singapore: Findings From a Pilot Vocational Rehabilitation Program.

Am J Occup Ther 2020 Nov/Dec;74(6):7406205040p1-7406205040p9

Miho Asano, PhD, is Assistant Professor, Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore;

Importance: Return to work after stroke is an important rehabilitation outcome. Vocational rehabilitation may support people with stroke to return to work.

Objective: To report the return-to-work rate of people with stroke who completed a local community-based vocational rehabilitation program and to describe the program's impact on their community reintegration.

Design: Retrospective database study.

Setting: Community-based voluntary welfare organization.

Participants: Fifty-eight men and women ages 21 to 55 yr, formally diagnosed with stroke, with no significant cognitive impairment, and fit to undergo rehabilitation.

Intervention: A pilot community-based interdisciplinary vocational rehabilitation program implemented between 2014 and 2017. The program included physical rehabilitation, psychosocial support, employment support, and caregiver support services.

Outcomes And Measures: Return-to-work rate and community reintegration as measured by the Community Integration Questionnaire (CIQ).

Results: Fifty participants completed the program. Their median age was 44 yr, and most were male (n = 37; 74%). Forty-four participants (88%) returned to work after completing the program. Statistically significant differences (p < .001) were found between their CIQ scores at program enrollment and at completion.

Conclusions And Relevance: The program appeared to benefit people with stroke in their return-to-work process. Future studies are recommended to determine the effectiveness of such programs and identify features contributing to their success. Potential implications for occupational therapy practice are that vocational rehabilitation interventions can be initiated earlier, comprehensive return-to-work programs can better address the unique needs of people with stroke, and interdisciplinary evaluations are needed to assess suitability for return to work.

What This Article Adds: One of occupational therapy's unique goals is to facilitate performance and participation in valued activities such as work. Occupational therapists can play a pivotal role across the continuum of care to support their clients to explore and achieve their vocational goals after stroke.
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http://dx.doi.org/10.5014/ajot.2020.041376DOI Listing
December 2020

Mean Platelet Volume/Platelet Count Ratio is Associated with Poor Clinical Outcome After Aneurysmal Subarachnoid Hemorrhage.

J Stroke Cerebrovasc Dis 2020 Nov 4;29(11):105208. Epub 2020 Aug 4.

Department of Neurosurgery, The First People's Hospital of Jingmen, Jingmen, Hubei, PR China. Electronic address:

Background: Aneurysmal subarachnoid hemorrhage (aSAH) is both a hypercoagulable and inflammation state in which many biomarkers have been studied. Activated platelets have been identified to be of clinical importance in thrombosis and neuroinflammation after aSAH. The aim of this study was to investigate the relationship between mean platelet volume (MPV) to platelet count (PC) ratio, a surrogate parameter for activated platelets, and the functional outcome in aSAH patients.

Methods: A retrospective analysis was performed of patients with aSAH admitted to the stroke center of our institution between November 2018 and November 2019. The mean MPV/PC ratio during the first three days after aSAH onset was calculated. Poor outcome was defined as a modified Rankin Scale (mRS) score of 3-6 at 3 months. Receive operating characteristic (ROC) curve analysis was performed to determine the optimal value of MPV/PC ratio for the prediction of poor outcome in patients with aSAH.

Results: A total of 100 patients were included, 13 (13.0%) died and 35 (35.0%) had a poor outcome. Mean MPV/PC ratio (P < 0.001) when measured over the study period, was significantly higher among patients with poor outcome. In multivariable analysis, increased mean MPV/PC ratio was associated with poor functional outcome at 3 months (odds ratio (OR) = 1.94; 95% confidence interval (CI): 1.19-3.17; P = 0.008). The optimal cutoff of MPV/PC ratio for predicting poor outcome at 3 months was 6.77 (sensitivity 74.3%, specificity 61.5%).

Conclusion: An increased MPV/PC ratio is associated with poor functional outcome in aSAH patients. MPV/PC ratio may be a useful predictor of outcome after aSAH.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105208DOI Listing
November 2020

Simultaneous determination of eight bioactive components in Marsdenia tenacissima extract in rat plasma by LC-MS/MS and its application in a pharmacokinetic study.

Biomed Chromatogr 2020 Nov 21;34(11):e4946. Epub 2020 Jul 21.

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, P. R. China.

As a traditional Chinese medicine, Marsdenia tenacissima (Roxb.) Wight et Arn. plays an indispensable role in clinical practice owing to its specific efficacy in treating malignant tumors, leukocythemia, cystitis and asthma. This study aimed to establish a novel and scientific LC-MS/MS approach to simultaneously determine tenacissoside B, H, G and I, caffeic acid, cryptochlorogenic acid, chlorogenic acid and neochlorogenic acid from M. tenacissima extract within the rat plasma samples. Digoxin was used as the internal reference. All determinations were carried out using the Eclipse Plus C column, and water (containing 0.1% formic acid) was used as the mobile phase A, while acetonitrile was the mobile phase B for gradient elution. The UPLC methods were validated, including calibration curves, accuracy, precision, stability and recovery of the total eight analytes, in accordance with the requirements for biopharmaceutical analysis. Moreover, the proposed approach was also used in comprehensive pharmacokinetic research on those eight analytes in rats following M. tenacissima extract gavage. According to the pharmacokinetic parameters, tenacissoside B, I, H and G are the long-acting and primary bioactive constituents in M. tenacissima extract, with long mean residence times and high concentrations. Our findings shed light on the absorption mechanism and provide significant information for the clinical application of M. tenacissima.
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http://dx.doi.org/10.1002/bmc.4946DOI Listing
November 2020

Spectrin couples cell shape, cortical tension, and Hippo signaling in retinal epithelial morphogenesis.

J Cell Biol 2020 04;219(4)

Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX.

Although extracellular force has a profound effect on cell shape, cytoskeleton tension, and cell proliferation through the Hippo signaling effector Yki/YAP/TAZ, how intracellular force regulates these processes remains poorly understood. Here, we report an essential role for spectrin in specifying cell shape by transmitting intracellular actomyosin force to cell membrane. While activation of myosin II in Drosophila melanogaster pupal retina leads to increased cortical tension, apical constriction, and Yki-mediated hyperplasia, spectrin mutant cells, despite showing myosin II activation and Yki-mediated hyperplasia, paradoxically display decreased cortical tension and expanded apical area. Mechanistically, we show that spectrin is required for tethering cortical F-actin to cell membrane domains outside the adherens junctions (AJs). Thus, in the absence of spectrin, the weakened attachment of cortical F-actin to plasma membrane results in a failure to transmit actomyosin force to cell membrane, causing an expansion of apical surfaces. These results uncover an essential mechanism that couples cell shape, cortical tension, and Hippo signaling and highlight the importance of non-AJ membrane domains in dictating cell shape in tissue morphogenesis.
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http://dx.doi.org/10.1083/jcb.201907018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147103PMC
April 2020

Exocyst Genes Are Essential for Recycling Membrane Proteins and Maintaining Slit Diaphragm in Nephrocytes.

J Am Soc Nephrol 2020 05 1;31(5):1024-1034. Epub 2020 Apr 1.

Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland

Background: Studies have linked mutations in genes encoding the eight-protein exocyst protein complex to kidney disease, but the underlying mechanism is unclear. Because nephrocytes share molecular and structural features with mammalian podocytes, they provide an efficient model for studying this issue.

Methods: We silenced genes encoding exocyst complex proteins specifically in nephrocytes and studied the effects on protein reabsorption by lacuna channels and filtration by the slit diaphragm. We performed nephrocyte functional assays, carried out super-resolution confocal microscopy of slit diaphragm proteins, and used transmission electron microscopy to analyze ultrastructural changes. We also examined the colocalization of slit diaphragm proteins with exocyst protein Sec15 and with endocytosis and recycling regulators Rab5, Rab7, and Rab11.

Results: Silencing exocyst genes in nephrocytes led to profound changes in structure and function. Abolition of cellular accumulation of hemolymph proteins with dramatically reduced lacuna channel membrane invaginations offered a strong indication of reabsorption defects. Moreover, the slit diaphragm's highly organized surface structure-essential for filtration-was disrupted, and key proteins were mislocalized. Ultrastructural analysis revealed that exocyst gene silencing led to the striking appearance of novel electron-dense structures that we named "exocyst rods," which likely represent accumulated membrane proteins following defective exocytosis or recycling. The slit diaphragm proteins partially colocalized with Sec15, Rab5, and Rab11.

Conclusions: Our findings suggest that the slit diaphragm of nephrocytes requires balanced endocytosis and recycling to maintain its structural integrity and that impairment of the exocyst complex leads to disruption of the slit diaphragm and nephrocyte malfunction. This model may help identify therapeutic targets for treating kidney diseases featuring molecular defects in vesicle endocytosis, exocytosis, and recycling.
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http://dx.doi.org/10.1681/ASN.2019060591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217423PMC
May 2020

Vulnerability of photosynthesis and photosystem I in Jerusalem artichoke (Helianthus tuberosus L.) exposed to waterlogging.

Plant Physiol Biochem 2018 Apr 17;125:239-246. Epub 2018 Feb 17.

Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.

Jerusalem artichoke (Helianthus tuberosus L.) is an important energy crop for utilizing coastal marginal land. This study was to investigate waterlogging tolerance of Jerusalem artichoke through photosynthetic diagnose with emphasis on photosystem II (PSII) and photosystem I (PSI) performance. Potted plants were subjected to severe (liquid level 5 cm above vermiculite surface) and moderate (liquid level 5 cm below vermiculite surface) waterlogging for 9 days. Large decreased photosynthetic rate suggested photosynthesis vulnerability upon waterlogging. After 7 days of severe waterlogging, PSII and PSI photoinhibition arose, indicated by significant decrease in the maximal photochemical efficiency of PSII (Fv/Fm) and PSI (△MR/MR), and PSI seemed more vulnerable because of greater decrease in △MR/MR than Fv/Fm. In line with decreased △MR/MR and unchanged Fv/Fm after 9 days of moderate waterlogging, the amount of PSI reaction center protein rather than PSII reaction center protein was lowered, confirming greater PSI vulnerability. According to positive correlation between △MR/MR and efficiency that an electron moves beyond primary quinone and negative correlation between △MR/MR and PSII excitation pressure, PSI inactivation elevated PSII excitation pressure by depressing electron transport at PSII acceptor side. Thus, PSI vulnerability induced PSII photoinhibition and endangered the stability of whole photosynthetic apparatus under waterlogging. In agreement with photosystems photoinhibition, elevated HO concentration and lipid peroxidation in the leaves corroborated waterlogging-induced oxidative stress. In conclusion, Jerusalem artichoke is a waterlogging sensitive species in terms of photosynthesis and PSI vulnerability. Consistently, tuber yield was tremendously reduced by waterlogging, confirming waterlogging sensitivity of Jerusalem artichoke.
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http://dx.doi.org/10.1016/j.plaphy.2018.02.017DOI Listing
April 2018

Superplastic Deformation Mechanisms of Superfine/Nanocrystalline Duplex PM-TiAl-Based Alloy.

Materials (Basel) 2017 Sep 19;10(9). Epub 2017 Sep 19.

School of Materials Science and Engineering, Changchun University of Technology, Changchun 130012, China.

In this paper, the equiaxed superfine/nanocrystalline duplex PM-TiAl-based alloy with (γ + α₂) microstructure, Ti-45Al-5Nb (at %), has been synthesized by high-energy ball milling and vacuum hot pressing sintering. Superplastic deformation behavior has been investigated at 1000 °C and 1050 °C with strain rates from 5 × 10 s to 1 × 10 s. The effects of deformation on the microstructure and mechanical behaviors of high Nb containing TiAl alloy have been characterized and analyzed. The results showed that, the ultimate tensile strength of the alloy was 58.7 MPa at 1000 °C and 10.5 MPa at 1050 °C with a strain rate of 5 × 10 s, while the elongation was 121% and 233%, respectively. The alloy exhibited superplastic elongation at 1000 and 1050 °C with an exponent (m) of 0.48 and 0.45. The main softening mechanism was dynamic recrystallization of γ grains; the dislocation slip and γ/γ interface twinning were responsible for superplastic deformation. The orientation relationship of γ/γ interface twinning obeyed the classical one: (001)//(110).
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http://dx.doi.org/10.3390/ma10091103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615756PMC
September 2017

Superplasticity of Annealed H13 Steel.

Materials (Basel) 2017 Jul 28;10(8). Epub 2017 Jul 28.

School of Materials Science and Engineering, Changchun University of Technology, Changchun 130012, China.

H13 steel is a widely used hot work die material. A new type of hot working method is imperative to develop complex and precise dies. In this paper, the heat treatment of H13 steel (AISI) was carried out by annealing, the final structure is a point or spherical pearlite, and the grain size is about 30-40 μm. The tensile properties of the annealed microstructure were investigated at 650, 750, and 850 °C with the strain rates of 1 × 10 s, 5 × 10 s, and 1 × 10 s. The tensile fracture and microstructure were analyzed by SEM and HREM. The results show that the tensile samples reach superplasticity at the strain rate of 1 × 10 s in the temperature range of 750-850 °C. When the temperature is 850 °C, the maximum elongation rate reaches 112.5%. This demonstrates the possibility of making superplastic forming molds. During the tensile process, the refined MC₆ and other high hardness carbides which are dispersed uniformly in the matrix, effectively inhibits grain growth and hinders dislocation movement, leading to the improvement of plasticity.
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http://dx.doi.org/10.3390/ma10080870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578236PMC
July 2017

A device design of an integrated CMOS poly-silicon biosensor-on-chip to enhance performance of biomolecular analytes in serum samples.

Biosens Bioelectron 2014 Nov 13;61:112-8. Epub 2014 May 13.

Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan; Graduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan. Electronic address:

For on-site clinical diagnosis of biomolecules, the detection performances of most point-of-care (POC) biosensor devices are limited by undesired cross-detection of other non-analyte proteins in patient serum samples and other complex samples. To conquer this obstacle, this work presents a fully integrated bottom-gate poly-silcion nanowire (polySi NW) biosensor system-on-chip (SoC) to enhance the detection performance of cardiac-specific troponin-I (cTnI) concentration levels in serum samples. By applying proper electrical potential at the bottom gate under polySi NW biosensor, the biosensor response to cTnI biomarker can be improved by at least 16 fold in 50% phantom serum samples. The experimental result shows its detection range is from 3.2 × 10(-13)M(mol l(-1)) to 3.2 × 10(-10)M. This enhancement can be attributed to the electrostatic interactions between target biomolecules and voltage-applied bottom gate electrodes. This is the first time that a polySi NW CMOS biosensor chip has shown feasibilities to detect specific biomarkers in serum samples. Therefore, the developed technology paves the way toward on-field applications of CMOS compatible SiNW biosensing technologies and it can be employed for future biomolecular analysis in on-site serum diagnosis applications.
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http://dx.doi.org/10.1016/j.bios.2014.05.010DOI Listing
November 2014

The biological function of the WD40 repeat-containing protein p55/Caf1 in Drosophila.

Dev Dyn 2012 Mar 12;241(3):455-64. Epub 2012 Jan 12.

National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing, China.

Background: The p55 family WD40 repeat-containing histone chaperone proteins are components of several chromatin regulatory complexes (such as PRC2, NURF and CAF-1) and interact with histone H4, yet their functional relevance in vivo is unclear.

Results: Here we use Drosophila as a genetic model to dissect the function of p55/Caf1 during development. In agree with a recent report, we find that p55 is essential for Drosophila development and is required for cell proliferation and viability. However, our data further demonstrate that histone H3K27 di-/tri-methylation and PRC2-mediated gene silencing still occur normally when p55 is missing. p55 is also implicated in bridging chromatin regulatory complexes to the chromatin by binding to histone H4, but we find that a transgene of p55 whose binding pocket is disrupted could still functionally substitute the wild-type p55 for the survival.

Conclusions: Our studies suggest that p55 is not crucial for PRC2-mediated gene silencing in vivo, and the vital function of p55 is probably not dependent on its interaction with histone H4.
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http://dx.doi.org/10.1002/dvdy.23730DOI Listing
March 2012

Synthesis of novel thiazolyl-pyrimidines and their anticancer activity in vitro.

Arch Pharm (Weinheim) 2011 Oct 29;344(10):675-83. Epub 2011 Aug 29.

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P.R. China.

A series of novel compounds 7-43 were prepared via the condensation of enaminones 4a-h and the guanidines carbonate 6a-f. The structures of these newly synthesized compounds were confirmed by (1) H-NMR, MS, EA and IR. All the compounds were tested for their cytotoxic activity in vitro against human cancer cell lines including Ishikawa, A549, BEL-7404, SPC-A-01 and SGC-7901. Most of them showed moderate cytotoxic against the tested cell lines. Among them, the most potent compounds 9 and 30 exhibited more efficient activity against Ishikawa, A549.
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http://dx.doi.org/10.1002/ardp.201000238DOI Listing
October 2011

[Graphite furnace atomic absorption spectrometry direct determination of urine thallium].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2008 Aug;26(8):486-7

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August 2008

Sparsity enhancement for blind deconvolution of ultrasonic signals in nondestructive testing application.

Rev Sci Instrum 2008 Jan;79(1):014901

Department of Information Measurement Technology and Instruments, Shanghai Jiaotong University, Shanghai 200240, People's Republic of China.

The received signal in ultrasonic pulse-echo inspection can be modeled as a convolution between an impulse response and the reflection sequence, which is the impulse characteristic of the inspected object. Deconvolution aims at approximately inverting this process to improve the time resolution so that the overlap between echoes from closely spaced reflectors becomes small. This paper presents a modified minimum entropy blind deconvolution algorithm for deconvolving ultrasonic signals. Enhancement of the resolution is achieved by using the presented method. In addition, the presented approach will, in many cases, lead to a faster computation. A nonlinear function is the key point to the efficiency of the modified blind deconvolution algorithm, which is used to increase the sparsity of the iteration output and to decrease the influence of the added noise by replacing each iteration output by output of the nonlinear function. Simulations showed the efficiency of the modification as compared with minimum entropy deconvolution when deconvolving synthetic ultrasonic signals. Experimental results using real ultrasonic data evaluated further that the exact solution consistently yields good performance. The thickness of a thin steel sample can be calculated by the modified blind deconvolution filter with a reasonable accuracy.
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http://dx.doi.org/10.1063/1.2836263DOI Listing
January 2008

3-[2-(Anilinocarbon-yl)eth-yl]-1-methyl-1H-imidazolium hexa-fluorido-phosphate.

Acta Crystallogr Sect E Struct Rep Online 2008 Mar 20;64(Pt 4):o721. Epub 2008 Mar 20.

College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.

The title compound, C(13)H(16)N(3)O(+)·PF(6) (-), which has an imide group in the imidazolium cation, is a new ionic liquid above its melting point. Two neighbouring mol-ecules are connected by a weak non-classical C-H⋯O hydrogen bond with the formation of centrosymmetric 14-membered dimers.
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http://dx.doi.org/10.1107/S1600536808005291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2961036PMC
March 2008

4-(3-Nitro-phen-yl)-3-(phenyl-sulfon-yl)but-3-en-2-one.

Acta Crystallogr Sect E Struct Rep Online 2008 Feb 29;64(Pt 3):o640. Epub 2008 Feb 29.

The C=C double bond in the title mol-ecule, C(16)H(13)NO(5)S, has an E configuration. The crystal structure is stabilized by C-H⋯O hydrogen bonds. There is also a weak C-H⋯π-ring inter-action in the structure.
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http://dx.doi.org/10.1107/S1600536808005370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2960772PMC
February 2008

Iron-catalyzed homocoupling of bromide compounds.

J Org Chem 2006 Aug;71(17):6637-9

College of Chemical Engineering and Materials and College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.

The homocoupling of bromide compounds was successfully performed in one pot by a combination of metallic magnesium and a catalytic amount of iron salts. The binary catalytic system differentiates itself from other homocoupling reactions catalyzed by iron salts in that it requires neither the in situ preparation of Grignard reagent nor the addition of a 1,2-dihalogen compound as an oxidant. Various aromatic and alkyl bromides underwent the homocoupling smoothly affording the corresponding symmetrical hydrocarbon compounds in moderate to excellent yields.
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http://dx.doi.org/10.1021/jo060673lDOI Listing
August 2006