Publications by authors named "Pei Wang"

1,238 Publications

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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesis.

Nat Commun 2021 06 18;12(1):3734. Epub 2021 Jun 18.

Translational Research Institute, Henan Provincial People's Hospital and People's Hospital of Zhengzhou University, Academy of Medical Science, Zhengzhou University, Henan, China.

Genomic amplification of the distal portion of chromosome 3q, which encodes a number of oncogenic proteins, is one of the most frequent chromosomal abnormalities in malignancy. Here we functionally characterise a non-protein product of the 3q region, the long noncoding RNA (lncRNA) PLANE, which is upregulated in diverse cancer types through copy number gain as well as E2F1-mediated transcriptional activation. PLANE forms an RNA-RNA duplex with the nuclear receptor co-repressor 2 (NCOR2) pre-mRNA at intron 45, binds to heterogeneous ribonucleoprotein M (hnRNPM) and facilitates the association of hnRNPM with the intron, thus leading to repression of the alternative splicing (AS) event generating NCOR2-202, a major protein-coding NCOR2 AS variant. This is, at least in part, responsible for PLANE-mediated promotion of cancer cell proliferation and tumorigenicity. These results uncover the function and regulation of PLANE and suggest that PLANE may constitute a therapeutic target in the pan-cancer context.
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http://dx.doi.org/10.1038/s41467-021-24099-4DOI Listing
June 2021

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues.

Clin Chem 2021 Jun 17. Epub 2021 Jun 17.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Background: Conventional HER2-targeting therapies improve outcomes for patients with HER2-positive breast cancer (BC), defined as tumors showing HER2 protein overexpression by immunohistochemistry and/or ERBB2 gene amplification determined by in situ hybridization (ISH). Emerging HER2-targeting compounds show benefit in some patients with neither HER2 protein overexpression nor ERBB2 gene amplification, creating a need for new assays to select HER2-low tumors for treatment with these compounds. We evaluated the analytical performance of a targeted mass spectrometry-based assay for quantifying HER2 protein in formalin-fixed paraffin-embedded (FFPE) and frozen BC biopsies.

Methods: We used immunoaffinity-enrichment coupled to multiple reaction monitoring-mass spectrometry (immuno-MRM-MS) to quantify HER2 protein (as peptide GLQSLPTHDPSPLQR) in 96 frozen and 119 FFPE BC biopsies. We characterized linearity, lower limit of quantification (LLOQ), and intra- and inter-day variation of the assay in frozen and FFPE tissue matrices. We determined concordance between HER2 immuno-MRM-MS and predicate immunohistochemistry and ISH assays and examined the benefit of multiplexing the assay to include proteins expressed in tumor subcompartments (e.g., stroma, adipose, lymphocytes, epithelium) to account for tissue heterogeneity.

Results: HER2 immuno-MRM-MS assay linearity was ≥103, assay coefficient of variation was 7.8% (FFPE) and 5.9% (frozen) for spiked-in analyte, and 7.7% (FFPE) and 7.9% (frozen) for endogenous measurements. Immuno-MRM-MS-based HER2 measurements strongly correlated with predicate assay HER2 determinations, and concordance was improved by normalizing to glyceraldehyde-3-phosphate dehydrogenase. HER2 was quantified above the LLOQ in all tumors.

Conclusions: Immuno-MRM-MS can be used to quantify HER2 in FFPE and frozen BC biopsies, even at low HER2 expression levels.
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http://dx.doi.org/10.1093/clinchem/hvab047DOI Listing
June 2021

Clinical application for DSA combined with a double-chamber Fogarty catheter in the treatment of venous crisis.

Wideochir Inne Tech Maloinwazyjne 2021 Jun 25;16(2):347-354. Epub 2020 Nov 25.

Department of Hand and Foot Surgery, Affiliated Hospital of Chengde Medical College, Chengde City, China.

Introduction: Venous crisis, as a common vascular crisis post limb replantation, is usually treated with surgical exploration.

Aim: To investigate effects of digital subtraction angiography (DSA) combined with double-chamber Fogarty balloon catheter on venous crisis post replantation of limbs.

Material And Methods: Twelve patients suffering from severed limbs were involved in this study. Patients underwent DSA combining double-chamber Fogarty balloon catheter operation. Colour Doppler ultrasound was used to diagnose patients with venous crisis. Patients were treated with rehydration, anti-infection, anticoagulation, and vasodilation. Indexes, including total joint active activity, working condition, remaining symptoms, appearance, feeling, and muscle strength, were evaluated.

Results: During operation, the limb was shortened to 0-1 cm in 8 cases, to 1-2 cm in 2 cases, and to 2-2.5 cm in 2 cases. According to DSA findings, popliteal vein thrombosis was formed at 0.6-4.2 cm and was removed from the popliteal vein. After removal of the thrombosis, DSA images showed re-canalization of the popliteal vein. A typical case of a 16-year-old patient underwent limb replantation; however, venous crisis was formed post operation. Postoperative colour Doppler ultrasound findings indicated re-canalization of the popliteal vein. Tibia and fibula were reduced and internally fixed, while the limb was survived post-operation. The degree of swelling of limbs was improved, and skin temperature was normal or 0.6-1.5°C lower than affected limbs. Skin colour was normal and activity was improved. Patients demonstrated sensory recovery grade of S and two-point discrimination of 4.5 mm.

Conclusions: DSA combining double-chamber Fogarty balloon catheter, as a minimally invasive and fast approach, could accurately locate thrombi and improve pertinence of vein branches.
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http://dx.doi.org/10.5114/wiitm.2020.101231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193746PMC
June 2021

A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV-2.

Front Cell Infect Microbiol 2021 28;11:680728. Epub 2021 May 28.

Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Key Laboratory of Marine Biological Resources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, School of Pharmacy, Jiangsu Ocean University, Lianyungang, China.

The pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to more than 117 million reported cases and 2.6 million deaths. Accurate diagnosis technologies are vital for controlling this pandemic. Reverse transcription (RT)-based nucleic acid detection assays have been developed, but the strict sample processing requirement of RT has posed obstacles on wider applications. This study established a ligation and recombinase polymerase amplification (L/RPA) combined assay for rapid detection of SARS-CoV-2 on genes N and ORF1ab targeting the specific biomarkers recommended by the China CDC. Ligase-based strategies usually have a low-efficiency problem on RNA templates. This study has addressed this problem by using a high concentration of the T4 DNA ligase and exploiting the high sensitivity of RPA. Through selection of the ligation probes and optimization of the RPA primers, the assay achieved a satisfactory sensitivity of 10 viral RNA copies per reaction, which was comparable to RT-quantitative polymerase chain reaction (RT-qPCR) and other nucleic acid detection assays for SARS-CoV-2. The assay could be finished in less than 30 min with a simple procedure, in which the requirement for sophisticated thermocycling equipment had been avoided. In addition, it avoided the RT procedure and could potentially ease the requirement for sample processing. Once validated with clinical samples, the L/RPA assay would increase the practical testing availability of SARS-CoV-2. Moreover, the principle of L/RPA has an application potential to the identification of concerned mutations of the virus.
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http://dx.doi.org/10.3389/fcimb.2021.680728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193850PMC
June 2021

Deep learning applications in automatic segmentation and reconstruction in CT-based cervix brachytherapy.

J Contemp Brachytherapy 2021 Jun 13;13(3):325-330. Epub 2021 May 13.

Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Radiation Oncology Key Laboratory of Sichuan Province, Chengdu, China.

Purpose: Motivated by recent advances in deep learning, the purpose of this study was to investigate a deep learning method in automatic segment and reconstruct applicators in computed tomography (CT) images for cervix brachytherapy treatment planning.

Material And Methods: U-Net model was developed for applicator segmentation in CT images. Sixty cervical cancer patients with Fletcher applicator were divided into training data and validation data according to ratio of 50 : 10, and another 10 patients with Fletcher applicator were employed to test the model. Dice similarity coefficient (DSC) and 95 percentile Hausdorff distance (HD95) were used to evaluate the model. Segmented applicator coordinates were calculated and applied into RT structure file. Tip error and shaft error of applicators were evaluated. Dosimetric differences between manual reconstruction and deep learning-based reconstruction were compared.

Results: The averaged overall 10 test patients' DSC, HD95, and reconstruction time were 0.89, 1.66 mm, and 17.12 s, respectively. The average tip error was 0.80 mm, and the average shaft error was less than 0.50 mm. The dosimetric differences between manual reconstruction and automatic reconstruction were 0.29% for high-risk clinical target volume (HR-CTV) D, and less than 2.64% for organs at risk D at a scenario of doubled maximum shaft error.

Conclusions: We proposed a deep learning-based reconstruction method to localize Fletcher applicator in three-dimensional CT images. The achieved accuracy and efficiency confirmed our method as clinically attractive. It paves the way for the automation of brachytherapy treatment planning.
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http://dx.doi.org/10.5114/jcb.2021.106118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170523PMC
June 2021

Switchable Assembly and Guidance of Colloidal Particles on an All-Dielectric One-Dimensional Photonic Crystal.

Phys Rev Appl 2020 Jan 13;13(1). Epub 2020 Jan 13.

Advanced Laser Technology Laboratory of Anhui Province and Institute of Photonics, Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.

Dielectric multilayer photonic-band-gap structures, called one-dimensional photonic crystals (1DPCs), have drawn considerable attention in the fields of physics, chemistry, and biophotonics. Here, experimental results verify the feasibility of a 1DPC working as a substrate for switchable manipulations of colloidal microparticles. The optically induced thermal convective force on a 1DPC can assemble colloidal particles that are dispersed in a water solution, while the photonic scattering force on the same 1DPC caused by propagating evanescent waves can guide these particles. Additionally, in the 1DPC, one internal mode can be excited that has seldom been noticed previously. This mode shows an ability to assemble particles over large areas even when the incident power is low. The assembly and guidance of colloidal particles on the 1DPC are switchable just through tuning the polarization and angle of the incident laser beam. Numerical simulations are carried out, which are consistent with these experimental observations.
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http://dx.doi.org/10.1103/physrevapplied.13.014020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189027PMC
January 2020

Optical Trapping with Focused Surface Waves.

Ann Phys 2020 Apr 24;532(4). Epub 2020 Feb 24.

Department of Optics and Optical Engineering, Institute of Photonics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Near-field optical trapping can be realized with focused evanescent waves that are excited at the water-glass interface due to the total internal reflection, or with focused plasmonic waves excited on the water-gold interface. Herein, the performance of these two kinds of near-field optical trapping techniques is compared using the same optical microscope configuration. Experimental results show that only a single-micron polystyrene bead can be trapped by the focused evanescent waves, whereas many beads are simultaneously attracted to the center of the excited region by focused plasmonic waves. This difference in trapping behavior is analyzed from the electric field intensity distributions of these two kinds of focused surface waves and the difference in trapping behavior is attributed to photothermal effects due to the light absorption by the gold film.
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http://dx.doi.org/10.1002/andp.201900497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189021PMC
April 2020

Hippo Signaling Pathway in Pancreas Development.

Front Cell Dev Biol 2021 17;9:663906. Epub 2021 May 17.

Department of Cell Systems and Anatomy, The University of Texas Health San Antonio, San Antonio, TX, United States.

The Hippo signaling pathway is a vital regulator of pancreatic development and homeostasis, directing cell fate decisions, morphogenesis, and adult pancreatic cellular plasticity. Through loss-of-function research, Hippo signaling has been found to play key roles in maintaining the proper balance between progenitor cell renewal, proliferation, and differentiation in pancreatic organogenesis. Other studies suggest that overactivation of YAP, a downstream effector of the pathway, promotes ductal cell development and suppresses endocrine cell fate specification via repression of Ngn3. After birth, disruptions in Hippo signaling have been found to lead to de-differentiation of acinar cells and pancreatitis-like phenotype. Further, Hippo signaling directs pancreatic morphogenesis by ensuring proper cell polarization and branching. Despite these findings, the mechanisms through which Hippo governs cell differentiation and pancreatic architecture are yet to be fully understood. Here, we review recent studies of Hippo functions in pancreatic development, including its crosstalk with NOTCH, WNT/β-catenin, and PI3K/Akt/mTOR signaling pathways.
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http://dx.doi.org/10.3389/fcell.2021.663906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165189PMC
May 2021

Duplex On-Site Detection of and by Recombinase Polymerase Amplification and Three-Segment Lateral Flow Strips.

Biosensors (Basel) 2021 May 12;11(5). Epub 2021 May 12.

Key Laboratory of Molecular Biophysics of Ministry of Education, Department of Biomedical Engineering, College of Life Science and Technology, Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan 430074, China.

and are two most reported foodborne pathogens related to seafood. Due to global ocean warming and an increase in seafood consumption worldwide, foodborne illnesses related to infection of these two bacteria are growing, leading to food safety issues and economic consequences. Molecular detection methods targeting species-specific genes are effective tools in the fight against bacterial infections for food safety. In this study, a duplex detection biosensor based on isothermal recombinase polymerase amplification (RPA) and a three-segment lateral flow strip (LFS) has been established. The biosensor used gene of and gene of as the detection markers based on previous reports. A duplex RPA reaction for both targets were constructed, and two chemical labels, FITC and DIG, of the amplification products were carefully tested for effective and accurate visualization on the strip. The biosensor demonstrated good specificity and achieved a sensitivity of 10 copies per reaction or one colony forming unit (CFU)/10 g of spiked food for both bacteria. Validation with clinical samples showed results consistent with that of real-time polymerase chain reaction. The detection process was simple and fast with a 30-min reaction at 37 °C and visualization on the strip within 5 min. With little dependence on laboratory settings, this biosensor was suitable for on-site detection, and the duplex system enabled simultaneous detection of the two important foodborne bacteria. Moreover, the principle can be extended to healthcare and food safety applications for other pathogens.
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http://dx.doi.org/10.3390/bios11050151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151630PMC
May 2021

Solar-Driven Lignocellulose-to-H Conversion in Water using 2D-2D MoS /TiO Photocatalysts.

ChemSusChem 2021 May 26. Epub 2021 May 26.

National Laboratory of Solid State Microstructures and Collaborative Innovation Center of Advanced Microstructures, Jiangsu Key Laboratory for Nano Technology, College of Engineering and Applied Science, Nanjing University, 210093, Nanjing, P. R. China.

As an alternative strategy for H production under ambient conditions, solar-driven lignocellulose-to-H conversion provides a very attractive approach to store and utilize solar energy sustainably. Exploiting efficient photocatalyst for photocatalytic lignocellulose-to-H conversion is of huge significance and remains the key challenge for development of solar H generation from lignocellulose. Herein, 2D-2D MoS /TiO photocatalysts with large 2D nanojunction were constructed for photocatalytic lignocellulose-to-H conversion. In this smart structure, the 2D nanojunctions acted as efficient channel for charge transfer from TiO to MoS to improve charge separation efficiency and thus enhance photocatalytic lignocellulose-to-H conversion activity. The 2 % MoS /TiO photocatalyst showed the highest photocatalytic lignocellulose-to-H conversion performance with the maximal H generation rate of 201 and 21.4 μmol h  g in α-cellulose and poplar wood chip aqueous solution, respectively. The apparent quantum yield at 380 nm reached 1.45 % for 2 % 2D-2D TiO /MoS photocatalyst in α-cellulose aqueous solution. This work highlights the importance of optimizing the interface structures of photocatalyst for solar-driven lignocellulose-to-H conversion.
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http://dx.doi.org/10.1002/cssc.202100829DOI Listing
May 2021

A community effort to identify and correct mislabeled samples in proteogenomic studies.

Patterns (N Y) 2021 May 7;2(5):100245. Epub 2021 May 7.

Lester and Sue Smith Breast Center, Baylor College of Medicine, Houston, TX 77030, USA.

Sample mislabeling or misannotation has been a long-standing problem in scientific research, particularly prevalent in large-scale, multi-omic studies due to the complexity of multi-omic workflows. There exists an urgent need for implementing quality controls to automatically screen for and correct sample mislabels or misannotations in multi-omic studies. Here, we describe a crowdsourced precisionFDA NCI-CPTAC Multi-omics Enabled Sample Mislabeling Correction Challenge, which provides a framework for systematic benchmarking and evaluation of mislabel identification and correction methods for integrative proteogenomic studies. The challenge received a large number of submissions from domestic and international data scientists, with highly variable performance observed across the submitted methods. Post-challenge collaboration between the top-performing teams and the challenge organizers has created an open-source software, COSMO, with demonstrated high accuracy and robustness in mislabeling identification and correction in simulated and real multi-omic datasets.
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http://dx.doi.org/10.1016/j.patter.2021.100245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134945PMC
May 2021

Phase I clinical trial of EGFR-specific CAR-T cells generated by the piggyBac transposon system in advanced relapsed/refractory non-small cell lung cancer patients.

J Cancer Res Clin Oncol 2021 May 25. Epub 2021 May 25.

Department of Biotherapy, The Eastern Hepatobiliary Surgery Hospital, Shanghai, 201805, China.

Purpose: This phase I clinical trial is designed to assess the safety and feasibility of the epidermal growth factor receptor (EGFR) chimeric antigen receptor (CAR) T-cell generated by the piggyBac transposon system in advanced relapsed/refractory non-small cell lung cancer (NSCLC) patients. Compared to viral systems, the piggyBac transposon system is a simpler, more economical, and alternative way to introduce chimeric antigen receptor (CAR) transgenes into T cells.

Methods: This study recruited nine patients with advanced relapsed/refractory EGFR-positive NSCLC for two cycles of the piggyBac-generated EGFR-CAR T cells at dose of 1 × 10 cells/kg or 3 × 10 cells/kg of body weight. The patients were monitored for adverse events, clinical response, and persistence of plasma GFR-CAR T cells.

Results: Infusions of piggyBac-generated EGFR-CAR T cells were well tolerated in all nine patients. The most common adverse events were grade 1 to 3 fever and there were no patients who experienced grade 4 adverse events or serious cytokine release syndrome. After treatment, eight of nine patients showed detectable EGFR-CAR T cells in their peripheral blood. One patient showed a partial response and lasted for more than 13 months, while six had stable disease, and two had progressed disease. The progression-free survival of these nine patients was 7.13 months (95% CI 2.71-17.10 months), while the median overall survival was 15.63 months (95% CI 8.82-22.03 months).

Conclusion: This Phase I clinical trial revealed that the non-viral piggyBac transposon system-engineered EGFR-CAR T-cell therapy is feasible and safe in treatment of EGFR-positive advanced relapsed/refractory NSCLC patients. Future study will assess it in more patients or even possibly with a higher dose. Trial registration number NCT03182816.
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http://dx.doi.org/10.1007/s00432-021-03613-7DOI Listing
May 2021

Could the EQ-5D-3L predict all-cause mortality in older Chinese? Evidence from a 5-year longitudinal study in eastern China.

Qual Life Res 2021 May 24. Epub 2021 May 24.

School of Public Health, Fudan University, 130 Dong An Road, Shanghai, 200032, China.

Purpose: To assess the ability of the 3-level EQ-5D (i.e., EQ-5D-3L) in predicting all-cause mortality in older Chinese adults.

Methods: The data were from a 5-year longitudinal study, Weitang Geriatric Diseases Study, including 4579 community-dwelling older people in eastern China, with the mean age of 72.5 years at baseline and female being 52.0%. Three multivariable logistic regression models were adopted to assess the associations of the baseline EQ-5D data [i.e., the EQ-5D problems, EQ-5D-3L index score, and EQ-5D visual analog scale (VAS) score] with the 5-year all-cause mortality, adjusting for socio-demographic characteristics, and subsequently, health conditions and lifestyle habits.

Results: A total of 183 participants died over the 5-year study period. A larger proportion of the dead reported problems in physical dimensions (i.e., including three dimensions: mobility, self-care, and usual activities, p < 0.05 for all). The mean EQ-5D index score (0.928) and EQ-VAS score (79.7) of the living were higher than those of the dead (0.915 and 73.2, p < 0.05 for both). In multivariable logistic analyses, the EQ-5D health problems in the physical-related dimensions [odds ratio (OR) 2.16, p < 0.05] and the EQ-VAS score (OR: 0.97, p < 0.001) were associated with the 5-year all-cause mortality when adjusting for socio-demographic characteristics, health conditions, and lifestyle habits.

Conclusions: It appears that the EQ-5D-3L could predict mortality in general older Chinese, which could be used to detect high-risk older individuals in China.
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http://dx.doi.org/10.1007/s11136-021-02883-5DOI Listing
May 2021

CUL4B Promotes Breast Carcinogenesis by Coordinating with Transcriptional Repressor Complexes in Response to Hypoxia Signaling Pathway.

Adv Sci (Weinh) 2021 05 16;8(10):2001515. Epub 2021 Mar 16.

Beijing Key Laboratory of Cancer Invasion and Metastasis Research Advanced Innovation Center for Human Brain Protection Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Capital Medical University Beijing 100069 China.

Cullin4B (CUL4B) is a scaffold protein of the CUL4B-Ring E3 ligase (CRL4B) complex. However, the role of CUL4B in the development of breast cancer remains poorly understood. Here it is shown that CRL4B interacts with multiple histone deacetylase (HDAC)-containing corepressor complexes, including MTA1/NuRD, SIN3A, CoREST, and NcoR/SMRT complexes. It is demonstrated that CRL4B/NuRD(MTA1) complexes cooccupy the E-cadherin and AXIN2 promoters, and could be recruited by transcription factors including Snail and ZEB2 to promote cell invasion and tumorigenesis both in vitro and in vivo. Remarkably, CUL4B responded to transformation and migration/invasion stimuli and is essential for multiple epithelial-mesenchymal transition (EMT) signaling pathways such as hypoxia. Furthermore, the transcription of CUL4B is directedly activated by hypoxia-inducible factor 1 (HIF1) and repressed by the ER-GATA3 axis. Overexpressing of CUL4B successfully induced CSC-like properties. Strikingly, CUL4B expression is markedly upregulated during breast cancer progression and correlated with poor prognosis. The results suggest that CUL4B lies at a critical crossroads between EMT and stem cell properties, supporting CUL4B as a potential novel target for the development of anti-breast cancer therapy.
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http://dx.doi.org/10.1002/advs.202001515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132058PMC
May 2021

Doctors inhibit social threat empathy in the later stage of cognitive processing: Electrophysiological evidence.

Conscious Cogn 2021 Jul 21;92:103130. Epub 2021 May 21.

Wenzhou Medical University, Wenzhou 325035, China. Electronic address:

Previous studies have found that repeatedly exposed to a threatening situation may reduce doctors' level of empathy, reducing psychological stress and avoiding burnout and compassion fatigue. However, many essential studies found that it does not seem universal but rather modulated by group membership. In this study, we recorded event-related potentials (ERP) when doctors and controls watched visual stimuli describing patients attacking doctors (Threat events) or shaking hands with doctors (Neutral events). The present study showed an early N190 and a later centro-parietal P3 differential amplitude between threat stimuli and neutral stimuli were observed in the controls. For the doctors, there was such ERP differentiation in early N190. However, later stage P3 differential amplitude was not observed. The current research suggests that doctors could regulate empathy and avoid allocating more attention resources when processing social threats to ensure treatment efficiency and avoid burnout.
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http://dx.doi.org/10.1016/j.concog.2021.103130DOI Listing
July 2021

Prognostic Value of the Tumor Immune Microenvironment for Early-stage, Non-Small Cell Lung Cancer.

Am J Clin Oncol 2021 Jul;44(7):350-355

Department of Population Health Science and Policy, Institute for Translational Epidemiology.

Introduction: The role of specific immune cell types within the tumor immune microenvironment in non-small cell lung cancer survival is unclear. The potential of these immune cells to become predictive biomarkers of prognosis, and to define subpopulations who will benefit of additional treatment is urgently needed.

Methods: Stage I to IIIA non-small cell lung cancer patients who underwent surgical resection were queried from the Cancer Genome Atlas; RNAseq data as well as clinical information was extracted. Sample-specific scores for different immune cells were computed via xCell. The association between each cell type and survival was assessed with Cox regression, both unadjusted and adjusted for sex, stage, smoking status, and tumor purity. Models were stratified by lung adenocarcinoma and lung squamous cell carcinoma.

Results: There were 383 lung adenocarcinoma and 328 lung squamous cell carcinoma samples, and 161 (42%) and 124 (38%) deaths respectively. There was no association between any immune cell infiltrations and survival in the combined unadjusted Cox regression model. After adjustment, the presence of CD8+ cytotoxic T cells (adjusted hazard ratio [HRajd]: 0.84; 95% confidence interval [CI]: 0.71-0.99; P=0.03), CD4+ helper T cells (HRajd: 0.79; 95% CI: 0.66-0.95; P=0.01) and CD20+ B cells (HRajd: 0.80; 95% CI: 0.66-0.97; P=0.02) were significant predictors of decreased risk of death.

Conclusions: This study shows that the adjustment for clinical characteristics is key when evaluating tumor immune infiltration and its association with cancer outcomes. Adjustment for confounding factors modified the prognostic significance of specific immune cell populations in early-stage surgically resected NSCLC cases; clinical attributes may have high relevance on immune infiltration composition.
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http://dx.doi.org/10.1097/COC.0000000000000832DOI Listing
July 2021

Staged angioplasty: A sensible approach to prevent hyperperfusion syndrome after carotid artery stenting? A meta-analysis.

Interv Neuroradiol 2021 May 18:15910199211018328. Epub 2021 May 18.

Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, China.

Objective: To investigate whether staged angioplasty (SAP) is a safe and effective treatment to prevent hyperperfusion syndrome after carotid artery stenting (CAS).

Methods: A systematic literature search was performed according to established criteria to identify eligible articles published before October 2020. Pooled dichotomous data were presented as odds ratios (OR) and corresponding 95% confidence intervals (CI) using random-effect models. The efficacy endpoints were hyperperfusion syndrome (HPS), hyperperfusion phenomenon (HPP), and intracerebral hemorrhage (ICH). The safety endpoint was post-procedural thromboembolic events. The feasibility of the procedure was assessed by device-related adverse events (vessel dissection and failed angioplasty) in SAP.

Results: Ten studies (1030 participants) were eligible. SAP was superior to regular CAS in preventing HPS (OR = 0.35, 95% CI 0.14-0.86, P = 0.02). There was no significant difference in the rate of thromboembolic events between the SAP group and the regular CAS group. The rates of vessel dissection and failed angioplasty with the use of a 3.0-mm-diameter balloon were 5.4% and 0.4%, respectively.

Conclusion: SAP may reduce the incidence of post-CAS HPS without increasing procedure-related complications. A 3.0-mm-diameter balloon used in SAP may be appropriate for Asian populations. However, the confounded study design and confused definitions of reporting items hinder the current recommendation of SAP in clinical use.
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http://dx.doi.org/10.1177/15910199211018328DOI Listing
May 2021

Selectively hydrolyzed soy protein as an efficient quality improver for steamed bread and its influence on dough components.

Food Chem 2021 Oct 22;359:129926. Epub 2021 Apr 22.

Institute of Agro-products Processing, Anhui Academy of Agricultural Science, Hefei 230031, China. Electronic address:

Selectively hydrolyzed soy protein (SHSP) has the potential to improve the quality of steamed bread. To clarify its underlying mechanism, the influence of SHSP on dough properties and components was investigated and compared with that of soy protein isolate (SPI). The results showed that SHSP addition resulted in steamed bread with higher loaf volume, lower hardness, and higher viscoelasticity. In contrast, SPI addition had the opposite effect. Nevertheless, both soy proteins decreased melting enthalpy and increased starch particle exposure due to competition for water. By analyzing molecular weight distribution and the secondary structure, we determined that the GMP content of fermented dough decreased by 10.04% following 1% SPI addition; however, it was enhanced by 7.90% following 1% SHSP addition. Moreover, the content of β-turns decreased with SHSP addition. The present study provides a theoretical basis for the exploitation of soy proteins as a nutritious and technofunctional dough improver.
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http://dx.doi.org/10.1016/j.foodchem.2021.129926DOI Listing
October 2021

Patient-caregiver agreement and test-retest reliability of the EQ-5D-Y-3L and EQ-5D-Y-5L in paediatric patients with haematological malignancies.

Eur J Health Econ 2021 May 5. Epub 2021 May 5.

Saw Swee Hock School of Public Health, National University of Singapore, 12 Science Drive 2, Singapore, Singapore.

Background: In 2019, the EuroQol Group developed a 'beta' 5-level version of EQ-5D-Y (Y-5L) by increasing the number of descriptive levels to five for each health dimension, as compared to the standard 3-level EQ-5D-Y (Y-3L).

Objective: To assess patient-caregiver agreement and test-retest reliability of the Y-5L and Y-3L in paediatric patients with haematological malignancies.

Methods: Paediatric inpatients aged 8-17 years were interviewed with the Y-5L and Y-3L questionnaires twice, while their caregivers were interviewed at the same time using the proxy versions of the questionnaires. Patient-caregiver agreement and test-retest reliability were assessed using Gwet's agreement coefficient (Gwet's AC1) for EQ-5D dimensions and the intraclass correlation coefficient (ICC) for the EQ VAS.

Results: Ninety-six patient-caregiver dyads participated in the study. Patient-caregiver agreement on the EQ-5D-Y descriptive system was moderate to good for both the Y-3L and Y-5L, but poor on the EQ VAS. Test-retest reliability of the descriptive system was good to very good for the Y-3L and moderate to good for the Y-5L in children, and fair to good for both versions of EQ-5D-Y in proxies. The EQ VAS showed good test-retest reliability in both children and caregivers. In a subgroup analysis of results in younger patients aged 8-10 years, patient-caregiver agreement and test-retest reliability were also observed to range from moderate to very good.

Conclusion: Both the Y-3L and Y-5L descriptive systems showed acceptable patient-caregiver agreement and test-retest reliability when used to assess the HRQoL of children and adolescents with haematological malignancies, including in younger patients.
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http://dx.doi.org/10.1007/s10198-021-01309-wDOI Listing
May 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Front Immunol 2021 14;12:663379. Epub 2021 Apr 14.

Division of Hematology-Oncology, Massachusetts General Hospital, Boston, MA, United States.

Immunotherapy for ovarian cancer is an area of intense investigation since the majority of women with relapsed disease develop resistance to conventional cytotoxic therapy. The paucity of safe and validated target antigens has limited the development of clinically relevant antibody-based immunotherapeutics for this disease. Although MUC16 expression is almost universal in High Grade Serous Ovarian Cancers, engagement of the shed circulating MUC16 antigen (CA-125) presents a theoretical risk of systemic activation and toxicity. We designed and evaluated a series of bispecific tandem single-chain variable fragments specific to the retained portion of human MUC16 ectodomain (MUC16) and human CD3. These MUC16- BiTEDs retain binding in the presence of soluble MUC16 (CA-125) and show cytotoxicity against a panel of ovarian cancer cells . MUC16- BiTEDs delay tumor progression and significantly prolong survival in a xenograft model of ovarian peritoneal carcinomatosis. This effect was significantly enhanced by antiangiogenic (anti-VEGF) therapy and immune checkpoint inhibition (anti-PD1). However, the combination of BiTEDs with anti-VEGF was superior to combination with anti-PD1, based on findings of decreased peritoneal tumor burden and ascites with the former. This study shows the feasibility and efficacy of MUC16- specific BiTEDs and provides a basis for the combination with anti-VEGF therapy for ovarian cancer.
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http://dx.doi.org/10.3389/fimmu.2021.663379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079980PMC
April 2021

Permissive effect of GSK3β on profibrogenic plasticity of renal tubular cells in progressive chronic kidney disease.

Cell Death Dis 2021 Apr 30;12(5):432. Epub 2021 Apr 30.

Division of Kidney Disease and Hypertension, Department of Medicine, Rhode Island Hospital, Brown University School of Medicine, Providence, RI, 02903, USA.

Renal tubular epithelial cells (TECs) play a key role in renal fibrogenesis. After persistent injuries that are beyond self-healing capacity, TECs will dedifferentiate, undergo growth arrest, convert to profibrogenic phenotypes, and resort to maladaptive plasticity that ultimately results in renal fibrosis. Evidence suggests that glycogen synthase kinase (GSK) 3β is centrally implicated in kidney injury. However, its role in renal fibrogenesis is obscure. Analysis of publicly available kidney transcriptome database demonstrated that patients with progressive chronic kidney disease (CKD) exhibited GSK3β overexpression in renal tubulointerstitium, in which the predefined hallmark gene sets implicated in fibrogenesis were remarkably enriched. In vitro, TGF-β1 treatment augmented GSK3β expression in TECs, concomitant with dedifferentiation, cell cycle arrest at G2/M phase, excessive accumulation of extracellular matrix, and overproduction of profibrotic cytokines like PAI-1 and CTGF. All these profibrogenic phenotypes were largely abrogated by GSK3β inhibitors or by ectopic expression of a dominant-negative mutant of GSK3β but reinforced in cells expressing the constitutively active mutant of GSK3β. Mechanistically, GSK3β suppressed, whereas inhibiting GSK3β facilitated, the activity of cAMP response element-binding protein (CREB), which competes for CREB-binding protein, a transcriptional coactivator essential for TGF-β1/Smad signaling pathway to drive TECs profibrogenic plasticity. In vivo, in mice with folic acid-induced progressive CKD, targeting of GSK3β in renal tubules via genetic ablation or by microdose lithium mitigated the profibrogenic plasticity of TEC, concomitant with attenuated interstitial fibrosis and tubular atrophy. Collectively, GSK3β is likely a pragmatic therapeutic target for averting profibrogenic plasticity of TECs and improving renal fibrosis.
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http://dx.doi.org/10.1038/s41419-021-03709-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087712PMC
April 2021

Exploring the influence mechanism of ozonation on protein fouling of ultrafiltration membranes as a result of the interfacial interaction of foulants at the membrane surface.

Sci Total Environ 2021 Sep 26;785:147340. Epub 2021 Apr 26.

Research Institute of Membrane Separation Technology of Shaanxi Province, Key Laboratory of Membrane Separation of Shaanxi Province, Key Laboratory of Northwest Water Resources, Key Laboratory of Environmental Engineering of Shaanxi Province, Xi'an University of Architecture and Technology, Yan Ta Road. No. 13, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Yan Ta Road. No. 13, Xi'an 710055, China.

Ozonation was widely used before ultrafiltration processes, but its effect mechanism on protein fouling is still controversial. Ozonation of bovine serum albumin (BSA) solutions was performed in the present work. The interfacial forces of BSA at the membrane surface were measured before and after ozonation. The adsorption behaviour of BSA onto the membrane surface and the fouling layer structures under different ozone dosages were also investigated. These results were combined with the membrane fouling behaviour to identify the effect of ozonation on protein fouling. The results showed that ozonation could weaken the interaction forces between the membrane and BSA effectively, but this did not have any effect on membrane fouling. In contrast, in terms of membrane fouling behaviour after pre-ozonation, the contribution of the changes in the covalent disulfide bonds between BSA molecules outweighs those of the non-covalent bonds. The number of disulfide bonds gradually increased as the O:DOC ratio increased from 0 to 0.3, and began to decline when the O:DOC ratio was further increased to 0.45 and 0.6. This could have altered the deposition rate of foulants onto the membrane surface and the structure of the fouling layers, and may have caused the membrane fouling first to be enhanced and then to decline with increasing ozone dosages.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147340DOI Listing
September 2021

Admission Serum Ionized and Total Calcium as New Predictors of Mortality in Patients with Cardiogenic Shock.

Biomed Res Int 2021 8;2021:6612276. Epub 2021 Apr 8.

Department of Cardiothoracic Surgery, Changzheng Hospital, Naval Medical University, Shanghai 200003, China.

Background: Although serum calcium has been proven to be a predictor of mortality in a wide range of diseases, its prognostic value in critically ill patients with cardiogenic shock (CS) remains unknown. This retrospective observational study is aimed at investigating the association of admission calcium with mortality among CS patients.

Methods: Critically ill patients diagnosed with CS in the Medical Information Mart for Intensive Care-III (MIMIC-III) database were included in our study. The study endpoints included 30-day, 90-day, and 365-day all-cause mortalities. First, admission serum ionized calcium (iCa) and total calcium (tCa) levels were analyzed as continuous variables using restricted cubic spline Cox regression models to evaluate the possible nonlinear relationship between serum calcium and mortality. Second, patients with CS were assigned to four groups according to the quartiles (Q1-Q4) of serum iCa and tCa levels, respectively. In addition, multivariable Cox regression analyses were used to assess the independent association of the quartiles of iCa and tCa with clinical outcomes.

Results: A total of 921 patients hospitalized with CS were enrolled in this study. A nonlinear relationship between serum calcium levels and 30-day mortality was observed (all values for nonlinear trend < 0.001). Furthermore, multivariable Cox analysis showed that compared with the reference quartile (Q3: 1.11 ≤ iCa < 1.17 mmol/L), the lowest serum iCa level quartile (Q1: iCa < 1.04 mmol/L) was independently associated with an increased risk of 30-day mortality (Q1 vs. Q3: HR 1.35, 95% CI 1.00-1.83, = 0.049), 90-day mortality (Q1 vs. Q3: HR 1.36, 95% CI 1.03-1.80, = 0.030), and 365-day mortality (Q1 vs. Q3: HR 1.28, 95% CI 1.01-1.67, = 0.046) in patients with CS.

Conclusions: Lower serum iCa levels on admission were potential predictors of an increased risk of mortality in critically ill patients with CS.
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http://dx.doi.org/10.1155/2021/6612276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049792PMC
May 2021

Disability-Free Life Expectancy among People Over 60 Years Old by Sex, Urban and Rural Areas in Jiangxi Province, China.

Int J Environ Res Public Health 2021 04 27;18(9). Epub 2021 Apr 27.

Jiangxi Province Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang 330006, China.

Objective: To estimate and compare age trends and the disability-free life expectancy (DFLE) of the population over 60 years old in 2018 in Jiangxi Province, China, by sex and urban-rural areas.

Methods: The model life table was employed to estimate the age-specific mortality rate by sex and urban-rural areas, based on the Summary of Health Statistics of Jiangxi Province in 2018 and the Sixth National Health Service survey of Jiangxi Province. DFLE and its ratio to life expectancy (LE) were obtained by the Sullivan method.

Results: In 2018, the DFLE among people over 60 is 17.157 years for men and is 19.055 years for women, accounting for 89.7% and 86.5% of their LE respectively. The DFLE/LE of men is higher than that of women at all ages. LE and DFLE are higher for the population in urban areas than in rural areas. For women, DFLE/LE is higher in urban areas than in rural areas (except at ages 75 and 80). Urban men have a higher DFLE/LE than rural men (except at age 85). The difference in DFLE between men and women over 60 years is 1.898 years, of which 2.260 years are attributable to the mortality rate, and 0.362 years are due to the disability-free prevalence. In addition, the difference in DFLE between urban-rural elderly over 60 years old is mostly attributed to the mortality rate by gender (male: 0.902/1.637; female: 0.893/1.454), but the impact of the disability-free rate cannot be ignored either (male: 0.735/1.637; female: 0.561/1.454).

Conclusions: The increase in DFLE is accompanied by the increase in LE, but with increased age, DFLE/LE gradually decreases. With advancing age, the effect of disability on elderly people becomes more severe. The government administration must implement some preventive actions to improve health awareness and the life quality of the elderly. Rural elderly; rural women in particular, need to be paid more attention and acquire more health care.
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http://dx.doi.org/10.3390/ijerph18094636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123896PMC
April 2021

Testing measurement properties of two EQ-5D youth versions and KIDSCREEN-10 in China.

Eur J Health Econ 2021 Apr 24. Epub 2021 Apr 24.

Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore.

Objective: To assess measurement properties of the two youth versions of EQ-5D (i.e., 5-level EQ-5D-Y [Y-5L] and 3-level EQ-5D-Y [Y-3L]) and KIDSCREEN-10 in China.

Methods: Children and adolescents attending schools in Shanghai, China were recruited to self-complete the Y-5L, KIDSCREEN-10, and Y-3L questionnaires. Their feasibility was assessed according to missing responses. Convergent validity of the EQ-5D-Y dimensions, a summated dimension score [SDS], and Visual Analogue Scale (VAS) were assessed by examining their correlations with the KIDSCREEN-10 index score and dimensions. Known-groups validity of SDS, VAS, and KIDSCREEN-10 index score were tested by comparing the scores of pupils with and without two conditions (i.e., overweight and shortsightedness), and the relative efficiency (RE) between them was also evaluated.

Results: A total of 262 pupils (girl: 58.4%; mean age: 12.7 years) were enrolled. Missing responses were low for both the Y-5L (0.3%) and Y-3L (2.4%), and KIDSCREEN-10 (0.3%). The overall ceiling effects were 40.3% for the Y-5L, 44.1% for the Y-3L and 1.1% for the KIDSCREEN-10. The SDS, SDS and VAS were moderately correlated with the KIDSCREEN-10 index score (|r|= 0.425 for SDS, 0.323 for SDS, and 0.435 for VAS; p < 0.01 for all). Similar EQ-5D-Y and KIDSCREEN-10 dimensions showed moderate to strong correlations (|r|> 0.3). Both the SDS and SDS had lower values, and VAS and KIDSCREEN-10 index score had higher values for pupils without shortsightedness compared with those for their counterparts. The difference was statistical significance for the SDS and VAS (P < 0.05 for both), which also had higher RE in the condition.

Conclusions: The Y-5L, Y-3L, and KIDSCREEN-10 questionnaires are feasible and valid for measuring HRQoL among children/adolescents in China. It also appears that the advantages of Y-5L over Y-3L were modest.
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http://dx.doi.org/10.1007/s10198-021-01307-yDOI Listing
April 2021

Integrating hydraulic tomography, electrical resistivity tomography, and partitioning interwell tracer test datasets to improve identification of pool-dominated DNAPL source zone architecture.

J Contam Hydrol 2021 Apr 7;241:103809. Epub 2021 Apr 7.

Key Laboratory of Surficial Geochemistry of Ministry of Education, School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China. Electronic address:

High-resolution characterization of complex dense non-aqueous phase liquid (DNAPL) contaminated sites is crucial for developing effective remediation strategies. The DNAPL source zone is usually characterized by hydraulic/partitioning tracer tomography (HPTT). However, the HPTT method may fail to capture the highly saturated pool-dominated DNAPL source zone architecture (SZA), because partitioning tracers tend to bypass the low-permeability zones where the pool DNAPL accumulates, resulting in a low-resolution DNAPL estimation. With a limited number of measurements, the estimation errors may be significant. To overcome these difficulties, time-lapse electrical resistivity tomography (ERT) was integrated with the partitioning interwell tracer test (PITT) and hydraulic tomography (HT) to characterize the pool-dominated DNAPL SZA. Herein, we proposed an iterative joint inversion framework coupling the multiphase flow model with the ERT forward model to estimate the heterogeneous permeability distribution and DNAPL SZA. Under this framework, permeability was estimated using the hydraulic head data from HT in stage 1, and the DNAPL SZA was subsequently estimated by assimilating both the PITT and ERT observations in stage 2. The permeability estimated from stage 1 was used as prior information for stage 2, and the DNAPL saturation estimated from stage 2 was served as prior information for stage 1 in the next loop to form an iterative loop to improve the estimation of both permeability and DNAPL SZA. The iterative joint inversion framework was evaluated in two numerical experiments with different heterogeneous structures by assimilating multi-source datasets, including hydraulic head, partitioning interwell tracer concentration, and electrical resistivity. Results show that with limited measurements of HPTT method, one can roughly capture the DNAPL distribution, missing the fine structure of the DNAPL SZA. In contrast, by incorporating multi-source datasets, the heterogeneous permeability and DNAPL SZA can be reconstructed with a higher resolution. Furthermore, the inversion accuracy of the DNAPL SZA improves progressively as the iteration proceeds, which demonstrates the advantage of utilizing complementary information from permeability and DNAPL distribution through the iteration framework. Comparing with the results without loop iteration, the estimation error is reduced by 17.3% for permeability and 8.6% for DNAPL saturation in Experiment 1; by 14.7% for permeability and 11.2% for DNAPL saturation in Experiment 2 through the iterative framework. To further evaluate our framework, we preformed the prediction of the depletion process of the DNAPL source zone and plume based on the estimated DNAPL SZA. Results show that using the iterative framework, the prediction of the SZA depletion is greatly improved, i.e., the estimation error of the dissolved downstream plume from the DNAPL source zone after 3 years is reduced by 20.9% in Experiment 1, and by 43.2% in Experiment 2, respectively, through the iterative framework. This significant improvement is because the iterative method can better capture the spread of DNAPL pool.
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http://dx.doi.org/10.1016/j.jconhyd.2021.103809DOI Listing
April 2021

Generation of pure longitudinal magnetization focal spot with a triplex metalens.

Opt Lett 2021 Apr;46(8):1896-1899

A pure longitudinal magnetization focal spot is greatly desired by all-optical magnetic recording. In this Letter, a triplex metalens is proposed and demonstrated to possess triple functions of an azimuthal polarization converter, a helical phase plate, and a focusing lens. The three-in-one combination enables conversion of linearly polarized incident light to the first-order azimuthally polarized vortex beam and focusing into a diffraction-limited spot. The state of polarization of the focal spot is measured to be pure circular polarization implying that a pure longitudinal magnetization field can be induced by the inverse Faraday effect. The lateral full width at half-maximum of the focal spot is 1.9 µm, and the experimental conversion efficiency of the metasurface is about 19.3%.
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http://dx.doi.org/10.1364/OL.422351DOI Listing
April 2021

Seasonal variations in water flux compositions controlled by leaf development: isotopic insights at the canopy-atmosphere interface.

Int J Biometeorol 2021 Apr 13. Epub 2021 Apr 13.

State Key Laboratory of Earth Surface Processes and Resource Ecology, Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China.

Water-stable isotopes provide a valuable tool for tracing plant-water interactions, particularly evapotranspiration (ET) partitioning and leaf water dynamics at the plant-atmosphere interface. However, process-based investigations of plant/leaf development and the associated isotopic dynamics of water fluxes involving isotope enrichment at plant-atmosphere interfaces at the ecosystem scale remain challenging. In this study, in situ isotopic measurements and tracer-aided models were used to study the dynamic interactions between vegetation growth and the isotopic dynamics of water fluxes (ET, soil evaporation, and transpiration) involving isotope enrichment in canopy leaves in a multispecies grassland ecosystem. The day-to-day variations in the isotopic compositions of ET flux were mainly controlled by plant growth, which could be explained by the significant logarithmic relationship determined between the leaf area index and transpiration fraction. Leaf development promoted a significant increase in the isotopic composition of ET and led to a slight decrease in the isotopic composition of water in canopy leaves. The transpiration (evaporation) isoflux acted to increase (decrease) the δO of water vapor, and the total isoflux impacts depended on the seasonal tradeoffs between transpiration and evaporation. The isotopic evidence in ET fluxes demonstrates the biotic controls on day-to-day variations in water/energy flux partitioning through transpiration activity. This study emphasizes that stable isotopes of hydrogen and oxygen are effective tools for quantitative evaluations of the hydrological component partitioning of ecosystems and plant-climate interactions.
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http://dx.doi.org/10.1007/s00484-021-02126-9DOI Listing
April 2021

Prednisone reduction for metastatic castration-resistant prostate cancer with recurrent pulmonary tuberculosis: Case report.

Medicine (Baltimore) 2021 Apr;100(15):e25584

Department of Radiation Oncology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.

Introduction: Prednisone (10 mg/d) is often used in combination with docetaxel or abiraterone in the treatment of advanced prostate cancer. LATITUDE studies have confirmed that the combination of abiraterone and prednisone (5 mg/d) can be used for the treatment of newly diagnosed high-risk metastatic castration-sensitive prostate cancer, and have achieved satisfactory results. However, it has not been reported that abiraterone combined with prednisone (5 mg/d) in the treatment of metastatic castration-resistant prostate cancer (mCRPC).

Patient Concerns: Here, we present a case of high-risk advanced prostate cancer with old pulmonary tuberculosis (PTB). The patient developed a relapse of old tuberculosis in both lungs that were discovered following 14 months of continuous application of prednisone (10 mg/d).

Diagnosis: The histopathological findings showed prostate adenocarcinoma carcinoma with a Gleason score of 10 (5+5). Further laboratory investigations were suggestive of positive mycobacterium tuberculosis complex DNA in pleural effusion and sputum.

Interventions: The patient underwent endocrine therapy, chemotherapy of docetaxel plus prednisone, radiotherapy, and abiraterone combined with prednisone treatment, but he eventually developed into the mCRPC stage. Then, prednisone was reduced to 5 mg/d plus abiraterone, and combined with anti-tuberculosis treatment according to multi-disciplinary diagnosis and treatment.

Outcome: Two months later, pleural effusion and atelectasis were relieved, and PSA was remained stable at a low level. The patient achieved complete remission.

Conclusion: We cannot, with complete certainty, say that this patient, or any patient, developed old PTB recurrence due to the use of prednisone. Based on the current evidence, endocrine therapy is the foundation, radiotherapy can reduce the tumor load, and early application of abiraterone is beneficial to survival for the high-risk mCRPC. The long-term use of prednisone can be appropriately reduced in mCRPC with old PTB, and a satisfactory curative effect can be achieved. More prospective trials are warranted before a definite recommendation could be drawn.
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http://dx.doi.org/10.1097/MD.0000000000025584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052077PMC
April 2021

Discovery of a Dimeric Zinc Complex and Five Cyclopentenone Derivatives from the Sponge-Associated Fungus .

ACS Omega 2021 Apr 26;6(13):8942-8949. Epub 2021 Mar 26.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.

In devotion to investigating structurally novel and biologically active marine natural products, a dimer of a zinc complex, dizinchydroxyneoaspergillin (), aspernones A-E (-), five cyclopentenone derivatives together with known polyketides (-), and neoaspergillic acid analogues (-) were isolated from the sponge-associated fungus SCSIO 41018. Their structures were elucidated on the basis of spectroscopic analysis, electronic circular dichroism (ECD) analysis, and X-ray diffraction. Dizinchydroxyneoaspergillin () displayed significant bactericide effects toward methicillin-resistant , , , , and with MIC values of 0.45-7.8 μg/mL and moderate in vitro cytotoxic activities against the K562, BEL-7402, and SGC-7901 cell lines with IC values of 12.88 ± 0.14, 15.83 ± 0.23, and 15.08 ± 0.62 μM, respectively. This is the first time to report the dimer of the zinc complex of hydroxyneoaspergillic acid conjunction at Zn-N-4 by a coordination bond. Additionally, compound displayed significant antibacterial and cytotoxic activities, which would be a promising drug lead and could attract much attention from both chemists and pharmacists.
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http://dx.doi.org/10.1021/acsomega.0c06218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028006PMC
April 2021