Publications by authors named "Pei Li"

722 Publications

Nomogram for Predicting the Relationship between the Extent of Visceral Pleural Invasion and Survival in Non-Small-Cell Lung Cancer.

Can Respir J 2021 24;2021:8816860. Epub 2021 May 24.

The Fourth Affiliated Hospital of Xinjiang Medical University, Urumqi 833000, Xinjiang, China.

Objective: Although visceral pleural invasion (VPI) has already been incorporated into the TNM staging system, few studies have been conducted to evaluate the prognostic value of the extent of VPI for the survival of non-small-cell lung cancer (NSCLC) patients. Thus, we utilized the Surveillance, Epidemiology, and End Results (SEER) database to assess the correlation between the extent of VPI and survival in NSCLC.

Methods: We identified and incorporated the extent of VPI to build a prognostic nomogram in this study. Patients in the SEER database diagnosed with NSCLC ( = 87,045) from 2010 to 2015 were further analyzed and randomly assigned into either the training group ( = 60,933) or validation group ( = 26,112). Clinical variables were calculated by means of multivariate Cox regressions and incorporated into the predictive model. Subsequently, the accuracy and discrimination of nomogram were further assessed through the concordance index (C-index), calibration curves, and Kaplan-Meier curves.

Results: Multivariate analysis demonstrated that the extent of visceral pleural invasion was an independent and unfavorable prognostic factor. The C-indexes of the training and validation groups were 0.772 (95% CI: 0.770-0.774) and 0.769 (95% CI: 0.765-0.773), respectively, which revealed that the nomogram had sufficient credibility and stable predictive accuracy. The calibration curve displayed consistency between the actual and predictive values in both training and validation groups.

Conclusion: The prognostic nomogram with the extent of VPI could offer an accurate risk evaluation for patients with NSCLC. Independent external validation of this research should be conducted in the future.
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http://dx.doi.org/10.1155/2021/8816860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169241PMC
May 2021

Molecular Characteristics of Causing Bloodstream Infections During 2010-2015 in a Tertiary Hospital, Shanghai, China.

Infect Drug Resist 2021 3;14:2079-2086. Epub 2021 Jun 3.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

Background: The bloodstream infections (BSI) caused by pose a serious threat to human health. To explore molecular characteristics of causing BSI, we collected isolates causing BSI in Huashan Hospital, Shanghai, China during 2010-2015.

Methods: In all isolates causing BSI collected from this study, polymerase chain reaction (PCR) was used to detect ESBLs and carbapenemase genes, and minimum inhibitory concentrations (MICs) were determined with agar dilution method. Outer membrane proteins were examined by SDS-PAGE in carbapenem-resistant strains. The genetic background of gene was investigated by combining next-generation sequencing with a PCR mapping approach. Conjugation and transformation experiments were performed to verify the mobilization of . The transcription levels of the gene were measured by RT-PCR.

Results: During 2010-2015, a total of 207 BSI strains were isolated. The positive rates of β-lactamase resistant genes were 0.48% (), 57% (), 23.67% (), 18.84% (), and 1.93% (). High rates of , and were consistent with the poor activity of third-generation cephalosporins and aztreonam in vitro, except for carbapenem and β-lactamase inhibitor combinations. Low susceptibility rates were observed for piperacillin (25.1%) in contrast to the increased susceptibility when combined with β-lactamase inhibitors, namely piperacillin-tazobactam (90.8%). Only one KPC-producing strain was detected. Despite the combination of OmpC loss, the low expression level of KPC may be responsible for its lower resistance to carbapenems compared to DH5α (pKP12-100).

Conclusion: strains isolated from BSI were still highly susceptible to carbapenems and β-lactamase inhibitor combinations, and was the dominant genotype of ESBLs. The low expression of may be the reason for the low resistance to carbapenems.
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http://dx.doi.org/10.2147/IDR.S305281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185459PMC
June 2021

Modulation of Intestinal Stem Cell Homeostasis by Nutrients: A Novel Therapeutic Option for Intestinal Diseases.

Nutr Res Rev 2021 Jun 8:1-28. Epub 2021 Jun 8.

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, People's Republic of China.

Intestinal stem cells, which are capable of both self-renewal and differentiation to mature cell types, are responsible for maintaining intestinal epithelial homeostasis. Recent evidence indicates that these processes are mediated, in part, through nutritional status in response to diet. Diverse dietary patterns including caloric restriction, fasting, high-fat diets, ketogenic diets and high-carbohydrate diets as well as other nutrients control intestinal stem cell self-renewal and differentiation through nutrient-sensing pathways such as mTOR and AMPK. Herein, we summarize the current understanding of how intestinal stem cells contribute to intestinal epithelial homeostasis and diseases. We also discuss the effects of diet and nutrient-sensing pathways on intestinal stem cell self-renewal and differentiation, as well as their potential application in the prevention and treatment of intestinal diseases.
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http://dx.doi.org/10.1017/S0954422421000172DOI Listing
June 2021

Novel Thiochromanone Derivatives Containing a Sulfonyl Hydrazone Moiety: Design, Synthesis, and Bioactivity Evaluation.

Molecules 2021 May 14;26(10). Epub 2021 May 14.

School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China.

A series of novel thiochromanone derivatives containing a sulfonyl hydrazone moiety were designed and synthesized. Their structures were determined by H-NMR, C-NMR, and HRMS. Bioassay results showed that most of the target compounds revealed moderate to good antibacterial activities against pv. , pv. , and pv. . Compound had the best inhibitory activity against pv. , pv. , and pv. , with the EC values of 8.67, 12.65, and 10.62 μg/mL, which were superior to those of Bismerthiazol and Thiodiazole-copper. Meanwhile, bioassay results showed that all of the target compounds proved to have lower antifungal activities against , , , , , and than those of Carbendazim.
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http://dx.doi.org/10.3390/molecules26102925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156870PMC
May 2021

A novel risk score for the prediction of airway management in patients with deep neck space abscess: a multicenter retrospective cohort study.

J Intensive Care 2021 May 20;9(1):41. Epub 2021 May 20.

Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou, Guangdong, People's Republic of China.

Background: Airway management, including noninvasive endotracheal intubation or invasive tracheostomy, is an essential treatment strategy for patients with deep neck space abscess (DNSA) to reverse acute hypoxia, which aids in avoiding acute cerebral hypoxia and cardiac arrest. This study aimed to develop and validate a novel risk score to predict the need for airway management in patients with DNSA.

Methods: Patients with DNSA admitted to 9 hospitals in Guangdong Province between January 1, 2015, and December 31, 2020, were included. The cohort was divided into the training and validation cohorts. The risk score was developed using the least absolute shrinkage and selection operator (LASSO) and logistic regression models in the training cohort. The external validity and diagnostic ability were assessed in the validation cohort.

Results: A total of 440 DNSA patients were included, of which 363 (60 required airway management) entered into the training cohort and 77 (13 required airway management) entered into the validation cohort. The risk score included 7 independent predictors (p < 0.05): multispace involvement (odd ratio [OR] 6.42, 95% confidence interval [CI] 1.79-23.07, p < 0.001), gas formation (OR 4.95, 95% CI 2.04-12.00, p < 0.001), dyspnea (OR 10.35, 95% CI 3.47-30.89, p < 0.001), primary region of infection, neutrophil percentage (OR 1.10, 95% CI 1.02-1.18, p = 0.015), platelet count to lymphocyte count ratio (OR 1.01, 95% CI 1.00-1.01, p = 0.010), and albumin level (OR 0.86, 95% CI 0.80-0.92, p < 0.001). Internal validation showed good discrimination, with an area under the curve (AUC) of 0.951 (95% CI 0.924-0.971), and good calibration (Hosmer-Lemeshow [HL] test, p = 0.821). Application of the clinical risk score in the validation cohort also revealed good discrimination (AUC 0.947, 95% CI 0.871-0.985) and calibration (HL test, p = 0.618). Decision curve analyses in both cohorts demonstrated that patients could benefit from this risk score. The score has been transformed into an online calculator that is freely available to the public.

Conclusions: The risk score may help predict a patient's risk of requiring airway management, thus advancing patient safety and supporting appropriate treatment.
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http://dx.doi.org/10.1186/s40560-021-00554-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139013PMC
May 2021

Design of steering wheel-type ring depressed-core 10-mode fiber with fully improved mode spacing.

Opt Express 2021 May;29(10):15067-15077

We present a steering wheel-type ring depressed-core few-mode fiber (SWTR-DC-FMF) that features a central depressed step-index core and a novel SWTR structure consisted of two symmetrical high-index parts and low-index parts, respectively. The DC and SWTR make great contribution to separate the non-degenerated LP modes and spatial modes in the circular symmetry core, resulting in fully improved mode spacing. The designed fiber is able to support 10 spatial modes with the minimum effective index difference (Min Δneff) between adjacent spatial modes larger than 1.93 × 10 and the Min Δneff between adjacent LP modes above 1.51 × 10 at the same time, facilitating potential fiber spatial mode multiplexing transmission with less multiple-input multiple-output (MIMO-less) digital signal processing technique. The broadband performance including n, Δneff, effective mode area (A) and differential mode delay (DMD) is comprehensively investigated over the whole C and L band. Moreover, the birefringence and fabrication tolerance are discussed. The designed fiber targets emerging applications in short-reach weakly coupled space-division multiplexing (SDM) optical networking to increase transmission capacity and spectral efficiency and further reduce the system complexity effectively.
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http://dx.doi.org/10.1364/OE.424554DOI Listing
May 2021

[Advances in the research of enterobacterial common antigen].

Sheng Wu Gong Cheng Xue Bao 2021 Apr;37(4):1081-1091

College of Veterinary Medicine, Southwest University, Chongqing 400715, China.

The enterobacterial common antigen (ECA) is a polysaccharide composed of polysaccharide repeats that are located in the outer membrane of almost all Enterobacteriaceae bacteria and has diverse biological functions. ECA is synthesized by the synergistic action of multiple genes that are present in clusters on the genome of Enterobacteriaceae bacteria, forming the ECA antigen gene cluster, an important virulence factor that plays a role in host invasion and survival of Enterobacteriaceae in vivo. ECA also plays an important role in the maintenance of the bacterial outer membrane permeability barrier, flagella gene expression, swarming motility, and bile salts resistance. In addition, ECALPS, anchored in the core region of bacterial lipopolysaccharide, is an important surface antigen for bacteria, stimulating high levels of antibody production in the host and could be a target for vaccine research. This review summarizes ECA purification, genes involved in ECA biosynthesis, its immunological characteristics, biological functions and clinical applications.
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http://dx.doi.org/10.13345/j.cjb.200334DOI Listing
April 2021

Chickpea Extract Ameliorates Metabolic Syndrome Symptoms via Restoring Intestinal Ecology and Metabolic Profile in Type 2 Diabetic Rats.

Mol Nutr Food Res 2021 May 8:e2100007. Epub 2021 May 8.

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.

Scope: Chickpeas have been recognized as a natural Uyghur medicine in Xinjiang (China) for 2500 years. Although the phenotypic effect on obesity or diabetes was authenticated, the mechanism was unclear. This work aims to study the effect of chickpea extract (CE) on metabolic syndrome induced by type 2 diabetes and to reveal its related mechanisms, focusing on intestinal flora and metabolomics.

Methods And Results: Diabetic rats are induced by a high-fat diet and intraperitoneal injection of streptozotocin. CE supplementation (3 g kg ) for 4 weeks improved the hyperglycemia, inflammatory state, and organ functions of diabetic rats. The metabolic profile trajectories of urine and faeces obtained by NMR have good separations among all groups, and CE significantly increases the contents of SCFAs in the cecum. Moreover, CE relieves intestinal dysbiosis by increasing the abundance of SCFAs-producing bacteria (e.g., Enterococcaceae) but reduces conditional pathogenic bacteria (e.g., Corynebacterium). PICRUSt predicts the functions of gut microbiome from the 16S rRNA gene sequences and metagenome, and finds that CE restored amino acids degradation, bile acids metabolism, and carbohydrate metabolism.

Conclusion: This study elucidates the role of CE from the perspective of metabolomics and the microbiota, which provides evidence for chickpea as a prebiotic to prevent diabetes.
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http://dx.doi.org/10.1002/mnfr.202100007DOI Listing
May 2021

Fixed versus flexible antagonist protocol in women with predicted high ovarian response except PCOS: a randomized controlled trial.

BMC Pregnancy Childbirth 2021 May 2;21(1):348. Epub 2021 May 2.

Chongqing Key Laboratory of Human embryo Engineering, Chongqing, China.

Background: No previous study directly compares the fixed day-5 initiation versus the flexible initiation of GnRH antagonist administration in IVF/ICSI for those patients who are predicted as high ovarian responders without PCOS. To evaluate whether the number of oocytes retrieved is different by using the two GnRH antagonist protocols in Chinese women with predicted high ovarian response except PCOS.

Methods: A randomized controlled trial of 201 infertile women with predicted high ovarian response except PCOS undergoing in vitro fertilization. Ovary stimulation was performed using recombinant FSH and GnRH antagonists. GnRH antagonist ganirelix (0.25 mg/d) was started either on day 5 of stimulation (fixed group) or when LH was > 10 IU/L, and/or a follicle with mean diameter > 12 mm was present, and/or serum E was > 600 pg/ml. Patient monitoring was initiated on day 3 of stimulation in flexible group.

Result(s): No significant difference was observed between the fixed and flexible groups regarding the number of oocytes retrieved (16.72 ± 7.25 vs. 17.47 ± 5.88, P = 0.421), the Gonadotropin treatment duration (9.53 ± 1.07 vs. 9.67 ± 1.03, P = 0.346) and total Gonadotropin dose (1427.75 ± 210.6 vs. 1455.94 ± 243.44, P = 0.381). GnRH antagonist treatment duration in fixed protocol was statistically longer than the flexible protocol (6.57 ± 1.17 vs 6.04 ± 1.03, P = 0.001). There was no premature LH surge in either protocol.

Conclusion(s): Fixed GnRH antagonist administration on day 5 of stimulation appear to achieve a comparable oocyte retrieved compared with flexible antagonist administration.

Trial Registration: NCT02635607 posted on December 16, 2015 in clinicaltrials.gov.
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http://dx.doi.org/10.1186/s12884-021-03833-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091520PMC
May 2021

A high-quality reference genome of wild Cannabis sativa.

Hortic Res 2020 May 2;7(1):73. Epub 2020 May 2.

Institute of Forensic Science, Ministry of Public Security, No. 17 South Muxidi Lane, Xicheng District, Beijing, 100038, China.

Cannabis sativa is a well-known plant species that has great economic and ecological significance. An incomplete genome of cloned C. sativa was obtained by using SOAPdenovo software in 2011. To further explore the utilization of this plant resource, we generated an updated draft genome sequence for wild-type varieties of C. sativa in China using PacBio single-molecule sequencing and Hi-C technology. Our assembled genome is approximately 808 Mb, with scaffold and contig N50 sizes of 83.00 Mb and 513.57 kb, respectively. Repetitive elements account for 74.75% of the genome. A total of 38,828 protein-coding genes were annotated, 98.20% of which were functionally annotated. We provide the first comprehensive de novo genome of wild-type varieties of C. sativa distributed in Tibet, China. Due to long-term growth in the wild environment, these varieties exhibit higher heterozygosity and contain more genetic information. This genetic resource is of great value for future investigations of cannabinoid metabolic pathways and will aid in promoting the commercial production of C. sativa and the effective utilization of cannabinoids. The assembled genome is also a valuable resource for intensively and effectively investigating the C. sativa genome further in the future.
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http://dx.doi.org/10.1038/s41438-020-0295-3DOI Listing
May 2020

LINC00941 Promotes Progression of Non-Small Cell Lung Cancer by Sponging miR-877-3p to Regulate VEGFA Expression.

Front Oncol 2021 31;11:650037. Epub 2021 Mar 31.

Department of Infectious Diseases, Affiliated Hospital 2 of Nantong University, Nantong, China.

Long noncoding RNAs (lncRNAs) play critical roles in carcinoma occurrence and metastasis. LINC00941 has been found to mediate the development of gastric cancer, and LINC00941 was negatively associated with the longer overall survival of lung adenocarcinoma patients. Herein, our aim was to investigate the effects and mechanisms of LINC00941 in NSCLC progression. Microarray was used to identify the change lncRNAs in NSCLC, LINC00941 was found to increase in tumor tissues and patients' plasma. Knockdown of LINC00941 didn't modulate the proliferation of NSCLC cells, but inhibition of LINC00941 in NSCLC cells suppressed the angiogenesis ability of human umbilical vein endothelial cells (HUVECs). Moreover, LINC00941 promoted tumorigenesis , while si-LINC00941 inhibited tumor development of NSCLC. VEGFA was should to be significantly modulated by LINC00941 in NSCLC cells, then luciferase assay proved that LINC00941 regulated VEGFA expression interacting with miR-877-3p. Followed functional experiments indicated that overexpression of LINC00941 accelerated angiogenesis and NSCLC tumor progression miR-877-3p/VEGFA axis both and . In conclusion, our results clarified the LINC00941 function for the first time, and LINC00941 promoted the progression of NSCLC, which was mediated by miR-877-3p/VEGFA axis. This study might provide new understanding and targets for NSCLC diagnosis and treatment.
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http://dx.doi.org/10.3389/fonc.2021.650037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044452PMC
March 2021

Prevalence and risk factors of children's dental anxiety in China: a longitudinal study.

BMJ Open 2021 04 16;11(4):e043647. Epub 2021 Apr 16.

Department of Pediatric Dentistry, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, China

Objectives: Dental anxiety remains widespread among children, may continue into adulthood and affect their oral health-related quality of life and clinical management. The aim of the study was to explore the trend of children's dental anxiety over time and potential risk factors.

Design: Longitudinal study.

Methods: Children aged between 5 and 12 years were investigated with the Chinese version of face version of Children's Fear Survey Schedule-Dental Subscale (CFSS-DS) and Frankl Behavior Rating scale from 2008 to 2017, and influential factors were explored.

Results: Clinical data were available from 1061 children, including 533 (50.2%) male participants and 528 (49.8%) female participants. The total CFSS-DS scores ranged from 16 to 66, with a mean of 24.8±10.3. The prevalence of dental anxiety is 11.59%. No significant differences in total CFSS-DS scores between girls and boys were found. According to the Frankl scale, 238 children were allocated to the uncooperative group and the remaining 823 children were allocated to the cooperative group. Scores of CFSS-DS were negatively correlated with the clinical behaviour level of Frankl. Children aged 11-12 years old had significantly decreased scores compared with other age groups, and there was a decline in the scores of the group aged 8-10 years old over time. The factor analysis divided 15 items of CFSS-DS into four factors, and the total scores of 'less invasive oral procedures' items belonging to factor III decreased significantly over time in the group aged 8-10 years old.

Conclusions: Age is a significant determinant for children's dental anxiety, and dental anxiety outcomes have improved for Chinese children aged 8-10 years. This study is one of the few reports on changes of children's dental anxiety in a new era of information, but the results may be extrapolated to other populations with caution.
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http://dx.doi.org/10.1136/bmjopen-2020-043647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055110PMC
April 2021

Identification of Spodoptera frugiperda importin alphas that facilitate the nuclear import of Autographa californica multiple nucleopolyhedrovirus DNA polymerase.

Insect Mol Biol 2021 Apr 9. Epub 2021 Apr 9.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.

Proteins containing nuclear localization signals (NLSs) are actively transported into the nucleus via the classic importin-α/β-mediated pathway, and NLSs are recognized by members of the importin-α family. Most studies of insect importin-αs have focused on Drosophila to date, little is known about the importin-α proteins in Lepidoptera insects. In this study, we identified four putative importin-α homologues, Spodoptera frugiperda importin-α1 (SfIMA1), SfIMA2, SfIMA4 and SfIMA7, from Sf9 cells. Immunofluorescence analysis showed that SfIMA2, SfIMA4 and SfIMA7 localized to the nucleus, while SfIMA1 distributed in cytoplasm. Additionally, SfIMA4 and SfIMA7 were also detected in the nuclear membrane of Sf9 cells. SfIMA1, SfIMA4 and SfIMA7, but not SfIMA2, were found to associate with the C terminus of AcMNPV DNA polymerase (DNApol) that harbours a typical monopartite NLS and a classic bipartite NLS. Further analysis of protein-protein interactions revealed that SfIMA1 specifically recognizes the bipartite NLS, while SfIMA4 and SfIMA7 bind to both monopartite and bipartite NLSs. Together, our results suggested that SfIMA1, SfIMA4 and SfIMA7 play important roles in the nuclear import of AcMNPV DNApol C terminus in Sf9 cells.
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http://dx.doi.org/10.1111/imb.12704DOI Listing
April 2021

Effects of intermittently scanned continuous glucose monitoring on blood glucose control and the production of urinary ketone bodies in pregestational diabetes mellitus.

Diabetol Metab Syndr 2021 Apr 9;13(1):39. Epub 2021 Apr 9.

NHC Key Laboratory of Hormones and Development (Tianjin Medical University), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin, 300134, China.

Objective: To investigate the effects of intermittently scanned continuous glucose monitoring (isCGM) on blood glucose control, clinical value of blood glucose monitoring and production of urinary ketone bodies in pregestational diabetes mellitus.

Method: A total of 124 patients with pregestational diabetes mellitus at 12-14 weeks of gestation admitted to the gestational diabetes clinic of our hospital from December 2016 to December 2018 were selected and randomly divided into two groups. Sixty patients adopted self-monitoring of blood glucose (SMBG) were taken as the control group, and the other 64 patients adopted isCGM system by wearing the device for 14 days. Blood sugar control, glycosylated albumin level, ketone production in urine, the maximum and minimum of blood sugar value measured by different monitoring methods and their occurrence time were observed in the two groups.

Result: (1) No statistically significant differences were found between the groups in terms of maternal age, gestational age at first visit, family history, duration of diabetes, education level, total insulin dose, chronic hypertension, abortion history, nulliparity, assisted reproductive technology, history of macrosomia childbirth, pre-pregnancy BMI, and overweight (%) at the first visit and hypoglycemia, (2) the value of Glycated Albumin was lower in the CGM group compared to the control group at 2ed weeks (14.6 ± 2.2 vs. 16.8 ± 2.7, p < 0.001). The women in the CGM group spent increased time in the recommended glucose control target range of 3.5-7.8 mmol/L (69 ± 10% vs. 62 ± 11%, p < 0.001) and reduced time above target compared with those in the control group at 2 weeks (25 ± 7% vs. 31 ± 8%, p < 0.001). In the second week of the study, the positive rate of urinary ketone body in isCGM group was lower than that in the control group (42 ± 5 vs. 54 ± 5, p < 0.001), and (3) the minimum blood glucose of 31.2% (20/64) cases in isCGM group appeared during 0:00-2:59 at night, and 26.6% (17/64) cases appeared during 3:00-5:59 at night. The minimum values of 40.0% (24/60) cases in the control group appeared within the 30 min before lunch, 23.3% (14/60) within the 30 min before breakfast, and 11.7% (7/60) within the 30 min before dinner. The cases of minimum of blood sugar before meals accounted for 75% of all the minimum values, and the cases of minimum at night only accounted for 8.3%.

Conclusion: Intermittently scanned continuous glucose monitoring can reduce hyperglycemia exposure and ketone body formation in pregestational diabetes mellitus. In addition, isCGM is better than SMBG in detecting nocturnal hypoglycemia.
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http://dx.doi.org/10.1186/s13098-021-00657-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034123PMC
April 2021

Application of portable real-time recombinase-aided amplification (rt-RAA) assay in the clinical diagnosis of ASFV and prospective DIVA diagnosis.

Appl Microbiol Biotechnol 2021 Apr 9;105(8):3249-3264. Epub 2021 Apr 9.

Beijing Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

African swine fever, a serious infectious disease, has been found in many countries around the world over the last nearly 100 years, and causes untold damage to the economy wherever it occurs. Diagnosis is currently performed by real-time PCR, which is highly sensitive but can only be carried out in a diagnostic laboratory environment with sophisticated equipment and expertise. A sensitive, rapid diagnostic method that can be implemented in agricultural settings is thus urgently needed for the detection and control of African swine fever virus (ASFV) infection. In this study, we developed an isothermal amplification technology to achieve molecular diagnosis of ASFV in clinical samples, using recombinase-aided amplification (RAA) assay combined with a portable instrument. This assay method avoids the limitations of traditional real-time PCR and offers detection times within 20 min, enabling detection of as few as 10 copies of ASFV DNA molecules per reaction without cross-reaction with other common swine viruses. We evaluated clinical performance using 200 clinical blood samples. The coincidence rate of the detection results between rt-RAA and RT-qPCR was 96.94% positive, 100% negative, and 97.50% total. We have also developed an rt-RAA system for the detection of ASFV targeting the EP402R gene, with detection of as few as 10 copies of DNA per reaction; this offers the possibility of DIVA (differentiating infected from vaccinated animals) diagnosis, because CD2V gene-deleted ASFV could soon be approved to be the leading candidate for live attenuated vaccine in China. The rt-RAA assay is a reliable, rapid, highly sensitive method, and it offers a reasonable alternative to RT-qPCR for point-of-care detection of ASFV. KEY POINTS: • The RT-RAA assay can detect as few as 10 copies of ASFV genome per reaction within 20 min. • The rt-RAA assay system targeting different genes can achieve differentiating infected from vaccinated diagnosis.
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http://dx.doi.org/10.1007/s00253-021-11196-zDOI Listing
April 2021

Mechanisms associated with increased physical activity in patients undergoing self-management behaviour modification in the randomised PHYSACTO trial.

ERJ Open Res 2021 Jan 29;7(1). Epub 2021 Mar 29.

University of Québec at Montréal/CIUSSS-NIM - Hôpital du Sacré-Coeur de Montréal, Montréal, Canada.

Introduction: In this analysis of the PHYSACTO® study, we assessed the efficacy of a self-management behaviour modification (SMBM) programme to improve physical activity (PA) levels, and the extent to which effects were mediated by readiness to change, motivation and confidence.

Methods: PHYSACTO® was a randomised, partially double-blind, parallel-group, 12-week trial to evaluate the effects of treatment on exercise capacity and PA. COPD patients received placebo, tiotropium 5 µg or tiotropium/olodaterol 5/5 µg, with or without exercise training, all with an SMBM intervention (the Living Well with COPD programme). Changes were assessed in readiness to change (stage of change visual analogue scale [VAS]), motivation (Treatment Self-Regulation Questionnaire [TSRQ]) and confidence (Perceived Competence Scale [PCS]) to engage in PA.

Results: PA was increased in all patients with complete PA data at Week 12 (n=262; +6038 steps·week, p<0.001). Significant increases were observed in patients' readiness to change (VAS 0.7 [0.6-0.8]), autonomous regulation (TRSQ 0.2 [0.1-0.3]) and confidence (PCS 0.5 [0.3-0.6]) (all p<0.01). Of note, 23% of the total effect of SMBM on steps·week was found to be mediated by increases in readiness to change, 5% by TSRQ autonomous regulation and 12% by PCS.

Conclusion: Our study demonstrated that an SMBM programme delivered to COPD patients increased PA, mediated by an improvement of three key hypothesised mechanisms of change: readiness to change, autonomous motivation and confidence. For the first time, this study shows that an SMBM programme can be successful in altering the mechanisms of change targeted by the intervention.
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http://dx.doi.org/10.1183/23120541.00533-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005679PMC
January 2021

Author Correction: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.

Nat Commun 2021 Apr 1;12(1):2144. Epub 2021 Apr 1.

NHC Key laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

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http://dx.doi.org/10.1038/s41467-021-22614-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016153PMC
April 2021

Unusual Maximum in the Adsorption of Aqueous Surfactant Mixtures: Neutron Reflectometry of Mixtures of Zwitterionic and Ionic Surfactants at the Silica-Aqueous Interface.

Langmuir 2021 Apr 28;37(13):3939-3949. Epub 2021 Mar 28.

Rutherford-Appleton Laboratory, Chilton, Didcot, Oxon OX11 0QX, U.K.

The adsorption of two zwitterionic surfactants, dodecyldimethylammonium propanesulfonate (CPS) and dodecyldimethylammonium carboxybetaine (CCB), and of their mixtures with the cationic dodecyltrimethylammonium bromide (CTAB) and the anionic sodium dodecylsulfate (SDS) at the silica-water interface has been studied by neutron reflection (NR). The total adsorption, the composition of the adsorbed layer, and some structural information have been obtained over a range of concentrations from below the critical micelle concentration (CMC) to about 30× the mixed CMC. The adsorption behavior has been considered in relation to the previously measured micellar equilibrium of these mixtures in their bulk solutions and their adsorption at the air-water interface. CCB adsorbs cooperatively close to its CMC to form an almost complete bilayer on its own, whereas CPS adsorbs more weakly in a fragmented bilayer structure. Although SDS does not normally adsorb at the silica-water interface, SDS adsorbs strongly and cooperatively with CPS at fractional SDS compositions up to about 0.5. This cooperativity is lost when the adsorbed fraction of SDS rises above about 0.5. At this point, adsorption drops sharply, creating an unusual maximum in the variation of adsorption with a total concentration above the mixed CMC. Neither the increase in cooperativity nor the subsequent decline in adsorption results directly from variations of the independently determined monomer concentrations in the bulk solution. The adsorption maximum is predominantly the effect of strong cooperative interaction, possibly accompanied by partial segregation of SDS within the layer, followed by charge repulsion from the surface. Although the solution aggregation and adsorption at the A-W interface are similar for SDS with CCB, the addition of SDS to CCB at the silica-water interface promotes the opposite behavior to that of SDS with CPS, and SDS simply disrupts the cooperative binding of CCB. Unlike SDS, the cationic surfactant CTAB adsorbs on silica. It therefore coadsorbs at the SiO-W interface with either CCB or CPS. However, in neither case is there any pronounced cooperativity and, even though the presence of CTAB might be expected to favor adsorption, the adsorption is generally unexpectedly low.
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http://dx.doi.org/10.1021/acs.langmuir.1c00178DOI Listing
April 2021

Hydrogen gas alleviates sepsis-induced neuroinflammation and cognitive impairment through regulation of DNMT1 and DNMT3a-mediated BDNF promoter IV methylation in mice.

Int Immunopharmacol 2021 Jun 24;95:107583. Epub 2021 Mar 24.

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin 300052, China; Tianjin Research Institute of Anesthesiology, Tianjin 300052, China. Electronic address:

Sepsis-associated encephalopathy (SAE) can cause acute and long-term cognitive impairment and increase the mortality rate in sepsis patients, and we previously reported that 2% hydrogen gas (H) inhalation has a therapeutic effect on SAE, but the underlying mechanism remains unclear. Dynamic DNA methylation, which catalyzed by DNA methyltransferases (DNMTs), is involved in the formation of synaptic plasticity and cognitive memory in the central nervous system. And brain-derived neurotrophic factor (BDNF), to be a key signaling component in activity-dependent synaptic plasticity, can be induced by neuronal activity accompanied by hypomethylation of its promoter IV. This study was designed to illustrate whether H can mediate SAE by alter the BDNF promoter IV methylation mediated by DNMTs. We established an SAE model by cecal ligation and perforation (CLP) in C57BL/6 mice. The Morris water maze test from the 4th to the 10th day after sham or CLP operations were used to evaluate mouse cognitive function. Hippocampal tissues were isolated at the 24 after sham or CLP surgery. Pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6) and High Mobility Group Box 1 (HMGB1) were measured by enzyme-linked immunosorbent assay (ELISA). mRNA or protein levels of DNMTs (DNMT1, DNMT3a and DNMT3b), BDNF promoter IV and total BDNF were detected by RT-PCR and Western blot tests. Immunofluorescence staining were used to determine the expressions of DNMT1 and DNMT3a. The quantitative methylation analysis of the 11 CpG island of the promoter region of BDNF exon IV was determined using theAgena's MassARRAY EpiTYPER system. We found that 2% H inhalation can reduce pro-inflammatory factors, alleviate DNMT1, DNMT3a but not DNMT3b expression, make hypomethylation of BDNF promoter IV at 5 CpG sites, enhance the BDNF levels and then decrease escape latency but increase platform crossing times in septic mice. Our results suggest that 2% H inhalation may alleviate SAE through altering the regulation of BDNF promoter IV methylation which mediated by DNMT1 and DNMT3a in the hippocampus of septic mice.
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http://dx.doi.org/10.1016/j.intimp.2021.107583DOI Listing
June 2021

Maternal vitamin D deficiency increases the risk of obesity in male offspring mice by affecting the immune response.

Nutrition 2021 Jul-Aug;87-88:111191. Epub 2021 Feb 11.

Department of Experimental Hematology and Biochemistry, Beijing Institute of Radiation Medicine, Beijing, China. Electronic address:

Objectives: Recently, many epidemiologic and animal studies have indicated that obesity has its origin in early stages of life, including the inappropriate balance of some nutrients. So the objectives of this study were to determine the risk of obesity in male offspring mice as a consequence of maternal vitamin D (VD) deficiency mediating the disordered immune response.

Methods: C57BL/6J female mice 4 wk old were fed VD-deficient or normal reproductive diets during pregnancy and lactation. Their male offspring were given control and high-fat diets for 16 wk after weaning and then weighed and euthanized. The serum was collected for biochemical analyses. Epididymal (eWAT) and inguinal white adipose tissue (iWAT) were excised for histologic examination, immunohistochemistry, gene expression of inflammatory factors, and determination by flow cytometry of the proportions of immune cells.

Results: Insufficient maternal VD intake exacerbated the development of obesity in male offspring mice that were both obese and non-obese, as evidenced by larger adipose cells and abnormal glucose and lipid metabolisms. Also, the expressions of proinflammatory cytokines were increased and that of anti-inflammatory cytokines was decreased in eWAT and/or iWAT in the maternal VD-deficient group, accompanied by higher levels of tumor necrosis factor-α and/or interferon-γ and lower levels of interleukin-4 and interleukin-10. Insufficient maternal VD intake was also observed to induce a shift in the profiles of immune cells in the eWAT and/or iWAT of male offspring that were both obese and non-obese, resulting in increased percentages of M1 macrophages, adipose tissue dendritic cells, and CD4 and CD8 T cells but a significant decrease in the percentages of M2 macrophages. All these changes in the immune cell profile were more obvious in the eWAT than those in the iWAT.

Conclusions: Maternal VD deficiency might promote the development of obesity in male offspring mice partly by modulating the immune cell populations and causing a polarization in the adipose depots.
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http://dx.doi.org/10.1016/j.nut.2021.111191DOI Listing
February 2021

Two-dimensional and three-dimensional echocardiographic assessment of right ventricular function in patients with pectus excavatum, before and after surgery.

Chin Med J (Engl) 2021 Mar 17;134(8):973-975. Epub 2021 Mar 17.

Heart Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.

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http://dx.doi.org/10.1097/CM9.0000000000001414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078358PMC
March 2021

Graphene-coated double D-type low loss optical fiber modulator.

Opt Express 2021 Jan;29(2):2025-2036

A graphene-coated double D-type low loss all-fiber modulator is proposed. The modulator is improved on the basis of standard fiber. Only the cladding is processed without grinding the original core structure. The upper and lower cladding are cut same distance. This can ensure that the mode field does not deviate in one direction, so that most of the mode field is still tied to the core, which greatly reduces the device loss. The existence of the double graphene layer can also ensure a very excellent modulation efficiency. The calculation results show that the mode loss of our proposed dual-D modulator under X polarization is 0.125 dB/mm, and the mode field mismatch loss is 0.25%. The mode loss in Y polarization is 0.033 dB/mm, and the mode field mismatch loss is 0.32%. When the modulation voltage is 5 V, the modulation depth is 78.4% under the condition of five-layer graphene, while the modulation speed can reach 15.38 GHz. Besides maintaining low modulation voltage and higher modulation efficiency, this structure makes full use of the advantages of good fiber coupling, and will be widely used in future fiber communications and all-fiber systems.
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http://dx.doi.org/10.1364/OE.413619DOI Listing
January 2021

Pathogenesis of Proteus mirabilis in Catheter-Associated Urinary Tract Infections.

Urol Int 2021 10;105(5-6):354-361. Epub 2021 Mar 10.

Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China,

Proteus mirabilis (PM) is a Gram-negative rod-shaped bacterium and widely exists in the natural environment, and it is most noted for its swarming motility and urease activity. PM is the main pathogen causing complicated urinary tract infections (UTIs), especially catheter-associated urinary tract infections. Clinically, PM can form a crystalline biofilm on the outer surface and inner cavity of the urethral indwelling catheter owing to its ureolytic biomineralization. This leads to catheter encrustation and blockage and, in most cases, is accompanied by urine retention and ascending UTI, causing cystitis, pyelonephritis, and the development of bladder or kidney stones, or even fatal complications such as septicemia and endotoxic shock. In this review, we discuss how PM is mediated by a catheter into the urethra, bladder, and then rose to the kidney causing UTI and the main virulence factors associated with different stages of infection, including flagella, pili or adhesins, urease, hemolysin, metal intake, and immune escape, encompassing both historical perspectives and current advances.
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http://dx.doi.org/10.1159/000514097DOI Listing
March 2021

A Review of Compact Carbon Design for Supercapacitors with High Volumetric Performance.

Small 2021 Mar 8:e2007548. Epub 2021 Mar 8.

Nanoyang Group, State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China.

Volumetric performance is of great importance in today's energy storage devices, and is used to evaluate their competitiveness in the markets of miniaturized electronic devices and space-constrained electric vehicles. Supercapacitors suffer from a low volumetric energy density in spite of their high power and long cycle life because of their use of porous but low-density carbons. This review considers compact carbon design strategies for high volumetric performance supercapacitors based on four key electrode parameters: density, thickness, gravimetric capacitance, and nonactive components. A guide is provided for constructing a conductive additive-/binder-free self-supported ultrathick, dense electrode to maximize the volumetric energy density. The research status of emerging micro-supercapacitors and hybrid supercapacitors is then briefly discussed, emphasizing the importance of their volumetric performance and the opportunities as well as challenges they face in the trendy Internet of things applications or larger device systems.
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http://dx.doi.org/10.1002/smll.202007548DOI Listing
March 2021

Fractionation and identification of salty peptides from yeast extract.

J Food Sci Technol 2021 Mar 12;58(3):1199-1208. Epub 2020 Oct 12.

Angel Yeast Co., Ltd., Yichang, 443000 Hubei Province China.

Abstract: Salty taste is an important sensory attribute in many foods, which stimulates the appetite. But high-salt diets bring many health risks, and salty alternatives should be explored to solve this problem. The salt-reducing agents may impart new odors in food. Therefore, the research should focus on developing a novel agent, which would replace the salt without affecting the taste of the food. Generally, some yeast extracts taste salty and can be used for replacing salts in foods without imparting any additional odor. In this study, we fractionated salty peptides from FA31 (Angel Yeast) by ultrafiltration, gel permeation chromatography, preparative liquid chromatography (pre-HPLC), with the combination of sensory evaluation, and the peptide sequence was identified by ESI-Q-TOF LC/MS as Asp-Asp, Glu-Asp, Asp-Asp-Asp, Ser-Pro-Glu, and Phe-Ile.

Graphic Abstract:
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http://dx.doi.org/10.1007/s13197-020-04836-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884503PMC
March 2021

Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway.

J Ophthalmol 2021 11;2021:6694050. Epub 2021 Feb 11.

Department of Geriatrics, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100043, China.

Background: Casein kinase 2-interacting protein-1 (CKIP-1) has been proved to be associated with complications of diabetes. Diabetic retinopathy is a main diabetic complication which usually leads to blindness. The current study aims to investigate the role of CKIP-1 in high glucose-treated retinal pigment epithelial (RPE) cells which is a component of blood-retinal barriers.

Methods: The RPE cells, ARPE-19, are treated with high glucose to mimic the diabetic stimulation. CKIP-1 was overexpressed in ARPE-19 cells to evaluate its effects on autophagy, oxidative stress, and apoptosis induced by high glucose treatment, using Western blot, immunofluorescence, and flow cytometry assays, respectively.

Results: CKIP-1 was expressed at a lower level in high glucose-treated cells than in normal glucose cells. Overexpression of CKIP-1 enhanced the Nrf2 translocation to the nucleus. Furthermore, high glucose-induced autophagy, oxidative stress, and apoptosis were inhibited after overexpression of CKIP-1. Also, CKIP-1 regulates the p62/Keap1/Nrf2 signaling, which might be the potential mechanism in this model.

Conclusion: In conclusion, CKIP-1 may be a potential therapeutic target that protects RPE cells from injury and subsequent diabetic retinopathy induced by high glucose.
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http://dx.doi.org/10.1155/2021/6694050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892229PMC
February 2021

An IncP-2 plasmid sublineage associated with dissemination of among carbapenem-resistant .

Emerg Microbes Infect 2021 Dec;10(1):442-449

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

IMP-45, a variant of IMP-9, is one of the dominant metallo-β-lactamases (MBLs) in clinical carbapenem-resistant (CRPA) isolates in China. The aim of this study was to investigate the distribution and mechanism of dissemination of . MBL genes were detected by PCR in 173 non-duplicate CRPA isolates collected from Hospital HS in Shanghai and 605 isolates from a multicenter surveillance of in China. In total, 17 IMP-45-producers (14 from Hospital HS and 3 from other hospitals) were identified. Molecular typing identified an outbreak of 11 IMP-45-producing ST508 CRPA in the ICU of Hospital HS. Conjugation assays and whole genome sequencing were conducted among IMP-45-producers. Genomic comparison revealed that 16 -carrying plasmids (9 from this study and 7 from GenBank) shared a similar backbone with IncP-2 -carrying plasmid pOZ176 but lacked -- region. gene was presented in pOZ176, -carrying plasmids (17 from this study and 7 from GenBank) and 15 megaplasmids from GenBank. Phylogenetic analysis of showed that most -carrying plasmids were clustered into a sublineage separate from the one containing pOZ176. This IncP-2 plasmid sublineage contributed to the dissemination of among genetically diverse and recruited multiple resistance genes during its evolution.
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http://dx.doi.org/10.1080/22221751.2021.1894903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971254PMC
December 2021

Aryl hydrocarbon receptor deficiency causes the development of chronic obstructive pulmonary disease through the integration of multiple pathogenic mechanisms.

FASEB J 2021 Mar;35(3):e21376

Research Institute of the McGill University Health Centre, Montreal, QC, Canada.

Emphysema, a component of chronic obstructive pulmonary disease (COPD), is characterized by irreversible alveolar destruction that results in a progressive decline in lung function. This alveolar destruction is caused by cigarette smoke, the most important risk factor for COPD. Only 15%-20% of smokers develop COPD, suggesting that unknown factors contribute to disease pathogenesis. We postulate that the aryl hydrocarbon receptor (AHR), a receptor/transcription factor highly expressed in the lungs, may be a new susceptibility factor whose expression protects against COPD. Here, we report that Ahr-deficient mice chronically exposed to cigarette smoke develop airspace enlargement concomitant with a decline in lung function. Chronic cigarette smoke exposure also increased cleaved caspase-3, lowered SOD2 expression, and altered MMP9 and TIMP-1 levels in Ahr-deficient mice. We also show that people with COPD have reduced expression of pulmonary and systemic AHR, with systemic AHR mRNA levels positively correlating with lung function. Systemic AHR was also lower in never-smokers with COPD. Thus, AHR expression protects against the development of COPD by controlling interrelated mechanisms involved in the pathogenesis of this disease. This study identifies the AHR as a new, central player in the homeostatic maintenance of lung health, providing a foundation for the AHR as a novel therapeutic target and/or predictive biomarker in chronic lung disease.
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http://dx.doi.org/10.1096/fj.202002350RDOI Listing
March 2021

Roles of Non-coding RNAs in Central Nervous System Axon Regeneration.

Front Neurosci 2021 1;15:630633. Epub 2021 Feb 1.

Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, China.

Axons in the central nervous system often fail to regenerate after injury due to the limited intrinsic regeneration ability of the central nervous system (CNS) and complex extracellular inhibitory factors. Therefore, it is of vital importance to have a better understanding of potential methods to promote the regeneration capability of injured nerves. Evidence has shown that non-coding RNAs play an essential role in nerve regeneration, especially long non-coding RNA (lncRNA), microRNA (miRNA), and circular RNA (circRNA). In this review, we profile their separate roles in axon regeneration after CNS injuries, such as spinal cord injury (SCI) and optic nerve injury. In addition, we also reveal the interactive networks among non-coding RNAs.
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http://dx.doi.org/10.3389/fnins.2021.630633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882506PMC
February 2021

Colorimetric detection of individual biothiols by tailor made reactions with silver nanoprisms.

Sci Rep 2021 Feb 16;11(1):3937. Epub 2021 Feb 16.

Department of Chemical and Biomolecular Engineering (BK 21+ Program), KAIST, Daehak-ro 291, Yuseong-gu, Daejeon, 34141, Republic of Korea.

We herein described a rapid, sensitive, and selective colorimetric sensing platform for biothiols in human serum, which relies on the dual functions of biothiols as anti-etching and aggregating agent for silver nanoprisms (AgNPRs). In principle, the target biothiols that bind to the surface of AgNPRs through Ag-S covalent interactions protect the AgNPRs from being etched by chloride ion (Cl) in human serum, thus exhibiting the blue/purple color that is indicative of AgNPRs. On the other hand, the color of AgNPRs turned to yellow in the absence of biothiols or the presence of non-sulfur-containing amino acids, indicating the formation of small silver nanoparticles (AgNPs). Importantly, we found that individual biothiols (Hcy, Cys, and GSH) exert not only the anti-etching effect, but also the aggregating effect on AgNPRs, which can be modulated by simply tuning the pH conditions, and this consequently allows for the discriminative detection of each biothiol. Based on this simple and cost-effective strategy, we successfully determined the Hcy, Cys, and GSH in human serum with high sensitivity and selectivity within 10 min, demonstrating the diagnostic capability and potential in practical applications.
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http://dx.doi.org/10.1038/s41598-021-83433-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886879PMC
February 2021