Publications by authors named "Pei Chen"

479 Publications

Electrically controlled spin reversal and spin polarization of electronic transport in nanoporous graphene nanoribbons.

Phys Chem Chem Phys 2021 Sep 13. Epub 2021 Sep 13.

College of Electronic and Optical Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

Based on first-principles calculations, the spin-dependent electronic transport of nanoporous graphene nanoribbons is investigated. A three-terminal configuration is proposed, which can electronically control the spin polarization of transmission, instead of magnetic methods. By modulating the gate voltage, not only could the transmission be switched between completely spin up and spin down polarized states to realize a dual-spin filter, but also the spin polarization could be finely tuned between 100% and -100%. Any ratio of spin up to spin down transport electrons can be realized, providing more possibilities for the design of nanoelectronic devices. Further analysis shows that the transmission spectra, with two distinct transmission peaks with opposite spins around , are the key point, which are contributed by p orbitals. And such a phenomenon is robust to the width and length of the nanoporous graphene nanoribbons, suggesting that it is an intrinsic feature of these systems. The electrical control on spin polarization is realized in pure-carbon systems, showing great application potential.
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http://dx.doi.org/10.1039/d1cp02547cDOI Listing
September 2021

Hydrogen sulfide alleviates salinity stress in Cyclocarya paliurus by maintaining chlorophyll fluorescence and regulating nitric oxide level and antioxidant capacity.

Plant Physiol Biochem 2021 Sep 8;167:738-747. Epub 2021 Sep 8.

Jiyang College, Zhejiang A&F University, Zhuji, Zhejiang, 311800, China; College of Forestry, Nanjing Forestry University, Nanjing, 210037, China. Electronic address:

Cyclocarya paliurus is commonly used to treat diabetes in China. However, the natural habitats of C. paliurus are typically affected by salt stress. Hydrogen sulfide (HS) is a growth regulator that is widely used to enhance plant stress tolerance, but the possible mechanism underlying HS-alleviated salt stress in C. paliurus remains unclear. C. paliurus seedlings pretreated with NaHS (an HS donor) were exposed to salt stress, and then, the leaf and total biomass, chlorophyll fluorescence parameters, nitric oxide (NO) content, oxidative damage, and proline and phenolic content were investigated to test the hypothesis that HS and NO were involved in the salt tolerance of C. paliurus. The results showed that HS pretreatment maintained chlorophyll fluorescence and attenuated the loss of plant biomass. We also found that HS pretreatment further increased the endogenous NO content and nitrate reductase activity compared with salt treatment. Moreover, HS pretreatment alleviated salt-induced oxidative damage, as indicated by lowered lipid peroxidation, through an enhanced antioxidant system including more proline and phenolic accumulation and increased antioxidant enzyme activities. However, C. paliurus leaves treated with the NO scavenger significantly diminished HS-mediated NO production and alleviation of membrane lipid peroxidation. Thus, we concluded that HS-induced NO was involved in C. paliurus salt tolerance.
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http://dx.doi.org/10.1016/j.plaphy.2021.09.004DOI Listing
September 2021

A new small-molecule compound, Q308, silences latent HIV-1 provirus by suppressing Tat- and FACT-mediated transcription.

Antimicrob Agents Chemother 2021 Sep 7:AAC0047021. Epub 2021 Sep 7.

Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou, 510515, PR China.

Eliminating the latent HIV reservoir remains a difficult problem for creating an HIV functional cure or achieving remission. The "block-and-lock" strategy aims to steadily suppress transcription of the viral reservoir and lock the HIV promoter in deep latency using latency-promoting agents (LPAs). However, to date, most of the investigated LPA candidates are not available for clinical trials, and some of them exhibit immune-related adverse reactions. The discovery and development of new, active, and safe LPA candidates for an HIV cure are necessary to eliminate residual HIV-1 viremia through the "block-and-lock" strategy. In this study, we demonstrated that a new small-molecule compound, Q308, silenced the HIV-1 provirus by inhibiting Tat-mediated gene transcription and selectively downregulating the expression levels of the facilitated chromatin transcription (FACT) complex. Strikingly, Q308 induced the preferential apoptosis in HIV-1 latently infected cells, indicating that Q308 may reduce the size of the viral reservoir and thus further prevent viral rebound. These findings highlight that Q308 is a novel and safe anti-HIV-1 inhibitor candidate for a functional cure.
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http://dx.doi.org/10.1128/AAC.00470-21DOI Listing
September 2021

Macrocycle-derived hierarchical porous organic polymers: synthesis and applications.

Chem Soc Rev 2021 Sep 7. Epub 2021 Sep 7.

Department of Chemistry, Tianjin Key Laboratory of Molecular Optoelectronic Science, Tianjin University, Tianjin 300072, China.

Porous organic polymers (POPs), as a new category of advanced porous materials, have received broad research interests owing to the advantages of light-weight, robust scaffolds, high specific surface areas and good functional tailorability. According to the long-range ordering of polymer skeletons, POPs can be either crystalline or amorphous. Macrocycles with inherent cavities can serve as receptors for recognizing or capturing specific guest molecules through host-guest interactions. Incorporating macrocycles in POP skeletons affords win-win merits, hierarchical porosity and novel physicochemical properties. In this review, we focus on the recent progress associated with new architectures of macrocycle-based POPs. Herein, these macrocycles are divided into two subclasses: non-planar (crown ether, calixarene, pillararene, cyclodextrin, cyclotricatechylene, ) and planar (arylene-ethynylene macrocycles). We summarize the synthetic methods of each macrocyclic POP in terms of the functions of versatile building blocks. Subsequently, we discuss the performance of macrocyclic POPs in environmental remediation, gas adsorption, heterogeneous catalysis, fluorescence sensing and ionic conduction. Although considerable examples are reported, the development of macrocyclic POPs is still in its infancy. Finally, we propose the underlying challenges and opportunities of macrocycle-based POPs.
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http://dx.doi.org/10.1039/d1cs00545fDOI Listing
September 2021

UHRF1 Suppresses HIV-1 Transcription and Promotes HIV-1 Latency by Competing with p-TEFb for Ubiquitination-Proteasomal Degradation of Tat.

mBio 2021 Aug 31;12(4):e0162521. Epub 2021 Aug 31.

Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.

HIV-1 remains incurable due to viral reservoirs, which lead to durably latent HIV infection. Identifying novel host factors and deciphering the molecular mechanisms involved in the establishment and maintenance of latency are critical to discover new targets for the development of novel anti-HIV agents. Here, we show that ubiquitin-like with PHD and RING finger domain 1 (UHRF1) modulates HIV-1 5'-long terminal repeat (LTR)-driven transcription of the viral genome as a novel HIV-1 restriction factor. Correspondingly, UHRF1 depletion reversed the latency of HIV-1 proviruses. Mechanistically, UHRF1 competed with positive transcription factor b (p-TEFb) for the binding to the cysteine-rich motifs of HIV-1 Tat via its TTD, PHD, and RING finger domains. Furthermore, UHRF1 mediated K48-linked ubiquitination and proteasomal degradation of Tat in RING-dependent ways, leading to the disruption of Tat/cyclin T1/CDK9 complex and consequential impediment of transcription elongation. In summary, our findings revealed that UHRF1 is an important mediator of HIV-1 latency by controlling Tat-mediated transcriptional activation, providing novel insights on host-pathogen interaction for modulating HIV-1 latency, beneficial for the development of anti-AIDS therapies. HIV-1 latency is systematically modulated by host factors and viral proteins. In our work, we identified a critical role of host factor ubiquitin-like with PHD and RING finger domain 1 (UHRF1) in HIV-1 latency via the modulation of the viral protein Tat stability. By disrupting the Tat/cyclin T1/CDK9 complex, UHRF1 promotes the suppression of HIV-1 transcription and maintenance of HIV-1 latency. Our findings provide novel insights in controlling Tat expression via host-pathogen interaction for modulating HIV-1 latency. Based on our results, modulating UHRF1 expression or activity by specific inhibitors is a potential therapeutic strategy for latency reversal in HIV-1 patients.
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http://dx.doi.org/10.1128/mBio.01625-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406157PMC
August 2021

Dynamic Network Biomarker of Pre-Exhausted CD8 T Cells Contributed to T Cell Exhaustion in Colorectal Cancer.

Front Immunol 2021 9;12:691142. Epub 2021 Aug 9.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

Immunotherapy has achieved positive clinical responses in various cancers. However, in advanced colorectal cancer (CRC), immunotherapy is challenging because of the deterioration of T-cell exhaustion, the mechanism of which is still unclear. In this study, we depicted CD8 T-cell developmental trajectories and characterized the pre-exhausted T cells isolated from CRC patients in the scRNA-seq data set using a dynamic network biomarker (DNB). Moreover, identified by DNB was a biomarker for pre-exhausted T-cell subpopulation in CRC. Besides, expression was triggered by as DNB core genes contributing to CD8 T cell exhaustion, indicating that core genes serve as biomarkers in pre-exhausted T cells. Remarkably, both and expressions were significantly associated with the overall survival of COAD patients in the TCGA database ( = 0.0082 and = 0.026, respectively). We also observed that cellular communication between terminally differentiated exhausted T cells and pre-exhausted T cells contributes to exhaustion. These findings provide new insights into the mechanism of T-cell exhaustion and provide clue for targeted immunotherapy in CRC.
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http://dx.doi.org/10.3389/fimmu.2021.691142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381053PMC
August 2021

Combined analysis of C-reactive protein in pleural fluid and serum is effective in the differential diagnosis of exudative pleural effusions.

Ann Transl Med 2021 Jul;9(14):1183

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Background: Exudative pleural effusion (EPE) is one of the common pleural manifestations of various diseases. Differential diagnosis of EPE is imperative clinically as it identifies different causes of EPE, thereby, enabling effective treatments. Thoracoscopy is a useful tool for differential diagnosis of EPE; however, some patients refuse thoracoscopic examination due to its invasive nature. In addition, the specificity and sensitivity of existing routine tests of EPE are unsatisfactory. Therefore, there is a great need to establish an effective method for the differential diagnosis of EPE.

Methods: This study was a single-institution retrospective analysis of diagnostic efficiency of C-reactive protein (CRP) and procalcitonin (PCT) between March 2018 and September 2018. A total of 87 patients diagnosed with EPE were enrolled. All participants underwent diagnostic thoracentesis. The EPE was examined using biochemical, routine, microbiological, and cytological methods. Pathological cytology detection was necessary for those suspected of malignant PE. Benign PE originates in patients with pneumonia, empyema, and tuberculosis. The levels of CRP and PCT in EPE and serum were measured before treatment. Correlation analysis and receiver-operating characteristic (ROC) curve analysis were conducted to determine the underlying relationship between levels of CRP and PCT, and for differential diagnosis.

Results: The ROC analysis showed that the sensitivity and specificity for the analysis of pleural fluid CRP (p-CRP) were higher (cut-off: 17.55 pg/mL; sensitivity: 75.00%, specificity: 83.90%) than that of serum CRP (s-CRP, cut-off: 23.90 pg/mL; sensitivity: 71.00%, specificity: 80.4%) in the differential diagnosis for EPE. However, the analysis of pleural fluid PCT (p-PCT) and serum PCT (s-PCT) did not demonstrate correlations with EPE. Combined analysis of p-CRP (cut-off: 17.55 mg/dL) with s-CRP (cut-off: 23.9 pg/mL) showed the highest diagnostic accuracy (88.4%) in diagnosing infectious EPE.

Conclusions: The data support the close relationship between combined analysis of p-CRP with s-CRP and effective and accurate differential diagnosis of EPE, due to its higher sensitivity and specificity. However, as a highly sensitive marker for diagnosing bacterial infections, neither s-PCT nor p-PCT, showed correlations with the differential diagnosis of EPE.
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http://dx.doi.org/10.21037/atm-21-3383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350706PMC
July 2021

Effect of the Spatial Configuration of Donors on the Photovoltaic Performance of Double D-π-A Organic Dyes.

ACS Appl Mater Interfaces 2021 Sep 24;13(34):40648-40655. Epub 2021 Aug 24.

Key Laboratory of Applied Surface and Colloid Chemistry, School of Materials Science and Engineering, Shannxi Normal University, Xi'an 710062, China.

Three double D-π-A sensitizers (, , and ) containing different donors (triphenylamine, methoxy-modified triphenylamine, and cyclic thiourea-functionalized triphenylamine) are synthesized to investigate the role of different donors in dye-sensitized solar cells (DSSCs). Detailed investigations of the sensitizers reveal that the spatial characteristics of donor units have a considerable impact on the light-harvesting, electrochemistry, and photovoltaic properties. Benefiting from the strong shielding ability of alkyl chains in the donor to its branch chains as observed in density functional theory (DFT), the open-circuit voltage ( = 712.0 mV) of -based DSSC is higher than those of and by 90 and 78 mV, respectively. Therefore, the -based DSSC delivers a good efficiency of 8.54%, relying on its effective suppression of interfacial recombination. The results indicate that the judiciously tailored donor unit is an effective approach to optimize dye configurations to further improve power conversion efficiencies.
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http://dx.doi.org/10.1021/acsami.1c11547DOI Listing
September 2021

A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.

J Food Biochem 2021 Sep 17;45(9):e13871. Epub 2021 Aug 17.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, PR China.

A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study. We researched its structure and thermal stabilities, and further studied its antioxidant activities in vitro. The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography. Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01. Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal. The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃. In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals. It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc). Therefore, it was revealed that HEP might be used as a natural antioxidant component. PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties. This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.
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http://dx.doi.org/10.1111/jfbc.13871DOI Listing
September 2021

Extraordinary phase coherence length in epitaxial halide perovskites.

iScience 2021 Aug 28;24(8):102912. Epub 2021 Jul 28.

Department of Physics and Astronomy, Michigan State University, East Lansing, MI 48824, USA.

Inorganic halide perovskites have emerged as a promising platform in a wide range of applications from solar energy harvesting to computing and light emission. The recent advent of epitaxial thin film growth of halide perovskites has made it possible to investigate low-dimensional quantum electronic devices based on this class of materials. This study leverages advances in vapor-phase epitaxy of halide perovskites to perform low-temperature magnetotransport measurements on single-domain cesium tin iodide (CsSnI) epitaxial thin films. The low-field magnetoresistance carries signatures of coherent quantum interference effects and spin-orbit coupling. These weak anti-localization measurements reveal a micron-scale low-temperature phase coherence length for charge carriers in this system. The results indicate that epitaxial halide perovskite heterostructures are a promising platform for investigating long coherent quantum electronic effects and potential applications in spintronics and spin-orbitronics.
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http://dx.doi.org/10.1016/j.isci.2021.102912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358163PMC
August 2021

CPI-637 as a Potential Bifunctional Latency-Reversing Agent That Targets Both the BRD4 and TIP60 Proteins.

Front Cell Infect Microbiol 2021 19;11:686035. Epub 2021 Jul 19.

Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.

The failure of highly active antiretroviral therapy (HAART) has been largely responsible for the existence of latent human immunodeficiency virus type 1 (HIV-1) reservoirs. The "shock and kill" strategy was confirmed to reactivate HIV-1 latent reservoirs by latency-reversing agents (LRAs) for accelerated HIV-1 clearance. However, a single LRA might be insufficient to induce HIV-1 reactivation from latency due to the complexity of the multiple signaling regulatory pathways that establish the HIV-1 latent reservoir. Therefore, combinations of LRAs or dual-mechanism LRAs are urgently needed to purge the latent reservoirs. We demonstrate here for the first time that a dual-target inhibitor with a specific suppressive effect on both BRD4 and TIP60, CPI-637, could reactivate latent HIV-1 by permitting Tat to bind positive transcription elongation factor b (P-TEFb) and assembling Tat-super-elongation complex (SEC) formation. In addition, CPI-637-mediated TIP60 downregulation further stimulated BRD4 dissociation from the HIV-1 long terminal repeat (LTR) promoter, allowing Tat to more effectively bind P-TEFb compared to BRD4 inhibition alone. Much more importantly, CPI-637 exerted a potent synergistic effect but alleviated global T cell activation and blocked viral spread to uninfected bystander CD4 T cells with minimal cytotoxicity. Our results indicate that CPI-637 opens up the prospect of novel dual-target inhibitors for antagonizing HIV-1 latency and deserves further investigation for development as a promising LRA with a "shock and kill" strategy.
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http://dx.doi.org/10.3389/fcimb.2021.686035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326664PMC
August 2021

A Prediction Model for Contralateral Central Neck Lymph Node Metastases in Unilateral Papillary Thyroid Cancer.

Int J Endocrinol 2021 30;2021:6621067. Epub 2021 Jun 30.

Department of General Surgery, Xiangya Hospital Central South University, Changsha 410008, Hunan, China.

The health problems caused by the frequent relapse of papillary thyroid carcinoma (PTC) remain a worldwide concern since the morbidity rate of PTC ranks the highest among thyroid cancers. Residues from contralateral central lymph node metastases (con-CLNM) are the key reason for persistence or recurrence of unilateral papillary thyroid carcinoma (uni-PTC); however, the ability to assess the status of con-CLNM in uni-PTC patients is limited. To clarify the risk factors of con-CLNM, a total of 250 patients with uni-PTC who underwent total thyroidectomy and bilateral central lymph node dissection were recruited in this study. We compared the clinical, sonographic, and pathological characteristics of patients with con-CLNM to those without con-CLNM and established a nomogram for con-CLNM in uni-PTC. We found that male sex, without Hashimoto's thyroiditis, present capsular invasion, with ipsilateral lateral lymph node metastases, and the ratio of ipsilateral central lymph node metastases ≥0.16 were independent con-CLNM predictors of uni-PTC (ORs: 2.797, 0.430, 2.538, 2.202, and 26.588; 95% CIs: 1.182-6.617, 0.211-0.876, 1.223-5.267, 1.064-4.557, and 7.596-93.069, respectively). Additionally, a preoperative nomogram for the prediction of con-CLNM based on these risk factors showed good discrimination (C-index 0.881; 95% CI: 0.840-0.923; sensitivity 85.3%; specificity 76.0%) and good agreement via the calibration plot. Our study provided a way to quantitatively and accurately predict whether con-CLNM occurred in patients with uni-PTC, which may guide surgeons to evaluate the nodal status and perform tailored therapeutic central lymph node dissection.
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http://dx.doi.org/10.1155/2021/6621067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263281PMC
June 2021

Retinoblastoma cell-derived exosomes promote angiogenesis of human vesicle endothelial cells through microRNA-92a-3p.

Cell Death Dis 2021 07 13;12(7):695. Epub 2021 Jul 13.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, No.7 Jinsui Road, Tianhe District, Guangzhou City, China.

Exosomes derived from tumor cells play a key role in tumor development. In the present study, we identified the bioactivity of exosomes released from WERI-Rb1 retinoblastoma cells in tumor angiogenesis, as well as the underlying mechanism, through biochemical methods and animal experiments. Our in vitro data showed that exosomes could be engulfed by human vesicle endothelial cells (HUVECs), significantly promote cell viability and induce an inflammatory response in HUVECs by increasing the expression of a series of related genes, such as IL-1, IL-6, IL-8, MCP-1, VCAM1, and ICAM1. Significant increases in migration and tube formation were also observed in the HUVECs incubated with exosomes. Moreover, experiments with a nude mouse xenotransplantation model showed that exosomes injected near tumors could be strongly absorbed by tumor cells. The numbers of endothelial cells and blood vessels were significantly increased in tumor tissues treated with exosomes compared to control tissues. Furthermore, to reveal the mechanism underlying exosome-mediated angiogenesis in retinoblastoma, we analyzed the levels of 12 microRNAs in the exosomes. Specifically, our data showed that miR-92a-3p was enriched in RB exosomes. Accordingly, miR-92a-3p was increased in the HUVECs incubated with these exosomes. After treatment with a miR-92a-3p inhibitor, the promoting effect of exosomes on the migration and tube formation of HUVECs was significantly abrogated. The expression of the angiogenesis-related genes mentioned above was markedly decreased in HUVECs. Similarly, treatment with a microRNA mimic also demonstrated that miR-92a-3p was involved in the angiogenesis of HUVECs. More importantly, bioinformatics analysis predicted that Krüppel-like factor 2 (KLF2), a member of the KLF family of zinc-finger transcription factors, might be an active target of miR-92a-3p. Notably, this prediction was confirmed both in vitro and in vivo. Thus, our work suggests that exosomal miR-92a-3p is involved in tumor angiogenesis and might be a promising therapeutic candidate for retinoblastoma.
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http://dx.doi.org/10.1038/s41419-021-03986-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277798PMC
July 2021

Forecasting the COVID-19 transmission in Italy based on the minimum spanning tree of dynamic region network.

PeerJ 2021 29;9:e11603. Epub 2021 Jun 29.

School of Mathematics, South China University of Technology, Guangzhou, China.

Background: Italy surpassed 1.5 million confirmed Coronavirus Disease 2019 (COVID-19) infections on November 26, as its death toll rose rapidly in the second wave of COVID-19 outbreak which is a heavy burden on hospitals. Therefore, it is necessary to forecast and early warn the potential outbreak of COVID-19 in the future, which facilitates the timely implementation of appropriate control measures. However, real-time prediction of COVID-19 transmission and outbreaks is usually challenging because of its complexity intertwining both biological systems and social systems.

Methods: By mining the dynamical information from region networks and the short-term time series data, we developed a data-driven model, the minimum-spanning-tree-based dynamical network marker (MST-DNM), to quantitatively analyze and monitor the dynamical process of COVID-19 spreading. Specifically, we collected the historical information of daily cases caused by COVID-19 infection in Italy from February 24, 2020 to November 28, 2020. When applied to the region network of Italy, the MST-DNM model has the ability to monitor the whole process of COVID-19 transmission and successfully identify the early-warning signals. The interpretability and practical significance of our model are explained in detail in this study.

Results: The study on the dynamical changes of Italian region networks reveals the dynamic of COVID-19 transmission at the network level. It is noteworthy that the driving force of MST-DNM only relies on small samples rather than years of time series data. Therefore, it is of great potential in public surveillance for emerging infectious diseases.
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http://dx.doi.org/10.7717/peerj.11603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253113PMC
June 2021

Propofol Mediated Protection of the Brain From Ischemia/Reperfusion Injury Through the Regulation of Microglial Connexin 43.

Front Cell Dev Biol 2021 8;9:637233. Epub 2021 Jun 8.

Department of Anesthesiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Cerebral ischemia/reperfusion (I/R) injury is a serious condition that leads to increased apoptosis of microglial and neurons in the brain. In this study, we identified that Cx43 expression level is significantly increased in the microglial cells during I/R injury. Using an model (hypoxia/reoxygenation-H/R injury), we observed that H/R injury leads to an increase in activation of microglial cells and increase in levels of pro-inflammatory markers such as IL-1β, IL-6, and TNF-α. Additionally, we could also observe significant increase in phosphorylation of Cx43 and Cav3.2 levels. To assess the role of H/R injured microglial cells on neuronal population, we cultured the neurons with conditioned media (MCS) from H/R injured microglial cells. Interestingly, we observed that microglial H/R injury significantly decreased Map2 expression and affected neuronal morphology. Further, we aimed to assess the effects of propofol on cerebral H/R injury, and observed that 40 μM propofol significantly decreased Cx43, Cx43 phosphorylation, and CaV3.2 levels. Additionally, propofol decreased apoptosis and increased Map2 expression levels in H/R injured neurons. Using silencing experiments, we confirmed that siCx43 could significantly improve the propofol's rescue after H/R injury in both microglia and neurons. We further developed an MCAO (middle cerebral artery occlusion) rat model to understand the effect of propofol in I/R injury. Interestingly, propofol treatment and downregulation of Cx43 significantly decreased the infract volume and apoptosis in these MCAO rats. Thus, this study clearly establishes that propofol protects the brain against I/R injury through the downregulation of Cx43 in microglial cells.
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http://dx.doi.org/10.3389/fcell.2021.637233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217990PMC
June 2021

CAV1 rs7804372 (T29107A) polymorphism might be a potential risk for digestive cancers: A protocol for systematic review and meta analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26186

Department of Medical Technology, Jiangsu College of Nursing, Huai an, Jiangsu, China.

Background: Caveolin-1 (CAV1) is an essential structural component of caveolae, regulates cellular processes through complex cellular signaling pathways, and influences tumorigenicity. However, the role of the CAV1 (rs7804372) polymorphism in digestive cancers remains inconclusive. The meta-analysis was performed to evaluate the effect of CAV1 polymorphism on digestive cancer susceptibility and to provide a basis for precise treatment.

Methods: The databases of PubMed, EMBASE, Google Scholar and CNKI were used to retrieve the published studies on CAV1 (rs7804372) polymorphism and susceptibility to digestive cancers up to June 2020. Two researchers conducted study screening, data extraction, and methodological quality evaluation separately according to inclusion and exclusion criteria. Review Manager 5.3 software was used to conduct the meta-analysis.

Results: Six case-control studies were enrolled, including 2477 patients with digestive cancers and 2477 healthy controls. The pooled results showed that the CAV1 rs7804372 (T29107A) polymorphism increased the risk of digestive cancer occurrence in the allele (T vs. A: odds ratio (OR) 1.33, 95% confidence interval (CI): 1.15-1.53, P < .01), homozygous (TT vs. AA: OR 1.72, 95% CI: 1.31-2.26, P < .01), heterozygous (TA vs. AA: OR 1.47, 95% CI: 1.21-1.78, P < .01), dominant (TT vs. TA + AA: OR 1.32, 95% CI: 1.18-1.48, P < .01), and recessive comparing models (TT + TA vs. AA: OR 1.61, 95% CI: 1.26-2.07, P < .01).

Conclusion: Our results indicate that the CAV1 (rs7804372) polymorphism may modify the occurrence of digestive cancers, and the presence of T allele or TT genotype of the CAV1 (rs7804372) may increase the risk of digestive cancers.
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http://dx.doi.org/10.1097/MD.0000000000026186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213254PMC
June 2021

Optimizing Safe Dental Practice During the COVID-19 Pandemic: Recommendations Based on a Guide Developed for Dental Practices in China.

Front Med (Lausanne) 2021 26;8:619357. Epub 2021 May 26.

Department of Periodontology and Oral Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China.

The current global coronavirus disease 2019 (COVID-19) outbreak is still exerting severe global implications, and its development in various regions is complex and variable. The high risk of cross-infection poses a great challenge to the dental practice environment; it is therefore urgent to develop a set of pandemic prevention measures to ensure dental practice safety during the COVID-19 outbreak. Therefore, we combined the epidemiological characteristics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), public emergency measures for COVID-19, characteristics of dental practice, and relevant literature reports to develop a set of dynamic practice measures for dental practices in high-, medium-, and low-risk areas affected by COVID-19. This will help dental practices to achieve standard prevention and ensure their safe and smooth operation during the pandemic. It is hoped that these measures will provide a reference basis for dental hospitals and dental clinics in their care and pandemic prevention work.
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http://dx.doi.org/10.3389/fmed.2021.619357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187590PMC
May 2021

Efficacy and safety of integrated traditional Chinese medicine and standard Western medicine for patients with acute coronary syndrome: protocol for a systematic review and meta-analysis.

Ann Palliat Med 2021 May;10(5):5763-5766

Hunan University of Chinese Medicine, Changsha, China.

Background: Acute coronary syndrome (ACS), which usually results from the buildup of fatty deposits (plaques) in and on the walls of coronary arteries, remains a major cause of morbidity and mortality worldwide. In the present systematic review, we aim to evaluate the efficacy and safety of adding traditional Chinese medicine (TCM) to standard western medicine to treat patients with ACS.

Methods: A systematic search was carried out in the China National Knowledge Infrastructure (CNKI; Chinese), the Chinese Biomedical Literature Disk Database CBMdisc (Chinese), Wanfang Data (Chinese), PubMed, Embase, and Web of Science for relevant papers up to January 20, 2021. All published randomized controlled trials related to the integration of TCM and Western medicine for ACS will be included. The primary outcome is the effective rate and cardiovascular function index (left ventricular ejection fraction, early peak flow velocity, and thrombolysis in myocardial infarction). The secondary outcome is blood lipid level (total cholesterol, triacylglycerol, low/high density lipoprotein) and the incidence of adverse cardiovascular events. Study selection, data collection, and quality evaluation will be conducted by 2 reviewers independently. Comprehensive meta-analyses together with sensitivity and subgroup analyses will be performed with Stata 15.0 software (StataCorp).

Discussion: The results will provide a high-quality guidance for the current clinical treatment of ACS, and the patients more options to relieve their symptoms. The findings from this study may provide updated evidence concerning the efficacy and safety of TCM for patients with ACS.

Trial Registration: This protocol has been registered in the INPLASY platform (https://inplasy.com/), and the registration number is INPLASY202140145.
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http://dx.doi.org/10.21037/apm-21-681DOI Listing
May 2021

Preharvest UVB Application Increases Glucosinolate Contents and Enhances Postharvest Quality of Broccoli Microgreens.

Molecules 2021 May 28;26(11). Epub 2021 May 28.

Beltsville Human Nutrition Research Center, Methods and Application of Food Composition Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA.

Broccoli microgreens have shown potential health benefits due to their high glucosinolate (GL) levels. Previously, we observed that postharvest UVB treatment did not have much effect on increasing GLs in broccoli microgreens. In this study, we investigated the influence of preharvest UVB irradiation on GL levels in broccoli microgreens. UHPLC-ESI/ITMS analysis showed that preharvest UVB treatments with UVB 0.09 and 0.27 Wh/m significantly increased the glucoraphanin (GLR), glucoerucin (GLE), and total aliphatic GL levels by 13.7 and 16.9%, respectively, in broccoli microgreens when measured on harvest day. The nutritional qualities of UVB-treated microgreens were stable during 21-day storage, with only small changes in their GL levels. Broccoli microgreens treated before harvest with UVB 0.27 Wh/m and 10 mM CaCl spray maintained their overall quality, and had the lowest tissue electrolyte leakage and off-odor values during the storage. Furthermore, preharvest UVB 0.27 Wh/m treatment significantly increased GL biosynthesis genes when evaluated before harvest, and reduced the expression level of myrosinase, a gene responsible for GL breakdown during postharvest storage. Overall, preharvest UVB treatment, together with calcium chloride spray, can increase and maintain health-beneficial compound levels such as GLs and prolong the postharvest quality of broccoli microgreens.
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http://dx.doi.org/10.3390/molecules26113247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197856PMC
May 2021

Preparation of Supported Perovskite Catalyst to Purify Membrane Concentrate of Coal Chemical Wastewater in UV-Catalytic Wet Hydrogen Peroxide Oxidation System.

Int J Environ Res Public Health 2021 05 4;18(9). Epub 2021 May 4.

College of Environmental Science and Engineering, Nanjing Tech University, Nanjing 211800, China.

The effective treatment of membrane concentrate is a major technical challenge faced by the new coal chemical industry. In this study, a supported perovskite catalyst LaCoO/X was prepared by a sol-impregnation two-step method. The feasibility of the supported perovskite catalyst LaCoO/X in the UV-catalytic wet hydrogen peroxide oxidation (UV-CWPO) system for the purification of concentrated liquid of coal chemical wastewater was investigated. The effects of catalyst support, calcination temperature, calcination time, and re-use time on catalytic performance were investigated by batch experiments. The catalysts were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and X-ray photoelectron spectroscopy (XPS). Experimental results showed that the supported perovskite catalyst LaCoO/CeO prepared using CeO as support, calcination temperature of 800 °C, and calcination time of 8 h had the best catalytic effect. The catalytic performance of the catalyst remained excellent after seven cycles. The best prepared catalyst was used in UV-CWPO of coal chemical wastewater membrane concentrate. The effects of HO dosage, reaction temperature, reaction pressure, and catalyst dosage on UV-CWPO were determined. Under the conditions of HO dosage of 40 mM, reaction temperature of 120 °C, reaction pressure of 0.5 MPa, catalyst dosage of 1 g/L, pH of 3, and reaction time of 60 min, the removal efficiencies of COD, TOC, and UV were 89.7%, 84.6%, and 98.1%, respectively. Under the optimal operating conditions, the oxidized effluent changed from high toxicity to non-toxicity, the BOD/COD increased from 0.02 to 0.412, and the biodegradability of the oxidized effluent was greatly improved. The catalyst has a simple synthesis procedure, excellent catalytic performance, and great potential in the practical application of coal chemical wastewater treatment.
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http://dx.doi.org/10.3390/ijerph18094906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125239PMC
May 2021

Phase I Trial on Arterial Embolization with Hypoxia Activated Tirapazamine for Unresectable Hepatocellular Carcinoma.

J Hepatocell Carcinoma 2021 17;8:421-434. Epub 2021 May 17.

Surgery Services, University of California Irvine, Orange, CA, USA.

Background: Tirapazamine (TPZ) is a hypoxia activated drug that may be synergistic with transarterial embolization (TAE). The primary objective was to evaluate the safety of combining TPZ and TAE in patients with unresectable HCC and determine the optimal dose for Phase II.

Methods: This was a Phase 1 multicenter, open-label, non-randomized trial with a classic 3+3 dose escalation and an expansion cohort in patients with unresectable HCC, Child Pugh A, ECOG 0 or 1. Two initial cohorts consisted of I.V. administration of Tirapazamine followed by superselective TAE while the remaining three cohorts underwent intraarterial administration of Tirapazamine with superselective TAE. Safety and tolerability were assessed using NCI CTCAE 4.0 with clinical, imaging and laboratory examinations including pharmacokinetic (PK) analysis and an electrocardiogram 1 day pre-dose, at 1, 2, 4, 6, 10, and 24 hours post-TPZ infusion and an additional PK at 15- and 30-minutes post-TPZ. Tumor responses were evaluated using mRECIST criteria.

Results: Twenty-seven patients (mean [range] age of 66.4 [37-79] years) with unresectable HCC were enrolled between July 2015 and January 2018. Two patients were lost to follow-up. Mean tumor size was 6.53 cm ± 2.60 cm with a median of two lesions per patient. Dose limiting toxicity and maximum tolerated dose were not reached. The maximal TPZ dose was 10 mg/m I.V. and 20 mg/m I.A. One adverse event (AE) was reported in all patients with fatigue, decreased appetite or pain being most common. Grade 3-5 AE were hypertension and transient elevation of AST/ALT in 70.4% of patients. No serious AE were drug related. Sixty percent (95% CI=38.7-78.9) achieved complete response (CR), and 84% (95% CI=63.9-95.5) had complete and partial response per mRECIST for target lesions.

Discussion: TAE with TPZ was safe and tolerable with encouraging results justifying pursuit of a Phase II trial.
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http://dx.doi.org/10.2147/JHC.S304275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139681PMC
May 2021

Corneal Epithelial Thickness Changes Following SMILE for Myopia With High Astigmatism.

J Refract Surg 2021 Apr 1;37(4):224-230. Epub 2021 Apr 1.

Purpose: To evaluate the corneal epithelial thickness (CET) profile changes after small incision lenticule extraction (SMILE) surgery for myopic astigmatism correction of greater than 2.00 diopters (D).

Methods: This prospective observational study included 40 eyes (23 patients) treated with SMILE for myopia with cylinders of -2.25 to -4.50 D. Along with standard ophthalmic examinations, CET maps with a diameter of 9 mm were measured by high-resolution spectral-domain optical coherence tomography preoperatively and postoperatively. Correlations between the degree of residual astigmatism and the difference in CET values between preoperative flat and steep meridians were analyzed.

Results: The CET showed significant changes in the central (2 mm), paracentral (2 to 5 mm), midperipheral (5 to 7 mm), and peripheral (7 to 9 mm) zones 6 months after SMILE ( < .001). Among the regions, the CET in the paracentral zones displayed the largest increase (9.75%) with the highest average thickness (57.29 µm). Moreover, symmetrical regional epithelial thickening at the preoperative astigmatism axis was observed in the midperipheral zones. The difference in CET between preoperative flat and steep meridians in the mid-peripheral zones continuously increased from postoperative 1 day to 6 months. This difference was positively correlated with the residual cylinder errors at 6 months postoperatively ( = -0.334, = .035).

Conclusions: The 9-mm diameter CET in eyes with high astigmatism significantly increased 6 months after SMILE. Additionally, the difference in CET between preoperative flat and steep meridians in midperipheral zones may be related to astigmatic undercorrection in SMILE. .
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http://dx.doi.org/10.3928/1081597X-20210126-01DOI Listing
April 2021

Fluctuations in Self-Reported Symptoms Predict Objective Physical Activity in Adults With Type 2 Diabetes.

Sci Diabetes Self Manag Care 2021 Aug 26;47(4):255-263. Epub 2021 May 26.

College of Nursing, University of Illinois at Chicago, Chicago, Illinois.

Purpose: The purpose of this study was to examine the association between self-reported symptoms including fatigue and sleep disturbance with moderate-intensity physical activity among adults with type 2 diabetes.

Methods: This report was a secondary analysis of a cross-sectional study. Data from 53 participants with at least 6 days of repeated measures were used. Daytime physical activity and energy expenditure were assessed using a wrist-worn accelerometer at the free-living setting. Fatigue upon awakening was measured using a 0 to 10 scale. Sleep (eg, restorative sleep, sleep duration, and sleep efficiency) was measured using the Consensus Sleep Diary for Morning. Data were analyzed using linear mixed models by including within- and between-person effects.

Results: Participants were predominantly females (54.7%) with a mean age of 60.3 years. Controlling for the covariates, at the individual level (within-person), fluctuations in restorative sleep and fatigue upon awakening predicted moderate-intensity PA. Similarly, at the individual level, fluctuations in restorative sleep and fatigue upon awakening predicted average hourly energy expenditure. However, at the group level (between-person), no significant associations were found between fatigue and restorative sleep with moderate-intensity physical activity.

Conclusions: The study findings suggest that within-person fluctuations in fatigue and restorative sleep upon awakening predict daytime moderate-intensity physical activity. At the individual level, reducing fluctuations in fatigue and restorative sleep might encourage participation in physical activity. More research is warranted to uncover the underlying causes of fluctuations in fatigue and restorative sleep. Meanwhile, diabetes care and education specialists should pay attention to the within-person fluctuations of fatigue and sleep.
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http://dx.doi.org/10.1177/26350106211015889DOI Listing
August 2021

FoxO1 in Micropterus salmoides: Molecular characterization and its roles in glucose metabolism by glucose or insulin-glucose loading.

Gen Comp Endocrinol 2021 Sep 9;310:113811. Epub 2021 May 9.

National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

Forkhead box O1 (FoxO1), a nuclear transcription factor, plays an important role in insulin-mediated glucose metabolism. In this study, FoxO1 gene from largemouth bass (Micropterus salmoides) was cloned and characterized, and its effects on hepatic glucose metabolism regulated by insulin-AKT pathway were investigated in response to glucose or insulin-glucose injection. The full-length cDNA of FoxO1 consisted of 2541 bp and encoded 680 amino acids. Sequence alignments and phylogenetic analysis revealed that FoxO1 exhibited a high degree of conservation among teleost, retaining one forkhead domain, one transactivation domain, and three phosphorylation sites. FoxO1 mRNA was expressed in a wide range of tissues, and high in the brain and liver. Glucose loading resulted in persistent hyperglycemia, and plasma insulin levels remained unchanged except at 1 h. After the insulin-glucose injection, insulin levels were significantly elevated and glucose levels recovered to the basal value after 6 h, which indicated insufficient insulin secretion caused persistent hyperglycemia in this species. Compared with the glucose injection group, transcript levels and enzyme activities of hepatic glycolysis-related genes (GK and PK) were significantly activated, and gluconeogenesis-related genes (PEPCK and G6Pase) were significantly depressed at 3 h after the insulin-glucose injection. Besides, phosphorylation of AKT-FoxO1 pathway was significantly activated. Therefore, insulin improved glucose metabolism by activating the AKT-FoxO1 phosphorylation  to decrease hyperglycemia stress after the meal, which indicated insufficient insulin secretion was the reason for glucose intolerance in largemouth bass. Meanwhile, conserved S267 and S329 phosphorylation sites of FoxO1 were confirmed to be regulated by AKT and mediated the glucose metabolism. In conclusion, activation of insulin-AKT-FoxO1 pathway improved glucose tolerance through mediating glucose metabolism in largemouth bass.
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http://dx.doi.org/10.1016/j.ygcen.2021.113811DOI Listing
September 2021

HTLV-1-Associated Lymphoma Presented as Massive Lymphadenopathy.

J Investig Med High Impact Case Rep 2021 Jan-Dec;9:23247096211013235

Brookdale University Hospital Medical Center, Brooklyn, NY, USA.

Adult T-cell leukemia/lymphoma is an aggressive T-cell malignancy caused by the long-term infection of human T-cell lymphotropic virus type 1 (HTLV-1). Our understanding of clinical features still largely relies on the Shimoyama classification developed 30 years ago, which described the 4 clinical subtypes (the smoldering, chronic, lymphoma, and acute types) based on the manifestations of lymphocytosis, elevated lactate dehydrogenase, hypercalcemia, lymphadenopathy, and involvement of the skin, lung, liver, spleen, central nervous system, bone, ascites, pleural effusion, and gastrointestinal tract. HTLV-1-associated lymphoma has a variety of presentations but the presentation of massive lymphadenopathy and compression symptoms is rare and has not been emphasized in the literature. In this article, we describe 2 cases of adult T-cell leukemia/lymphomas that presented with massive cervical nodes or mediastinal nodes with compressing symptoms as the major presenting clinical features. Clinicians should remain aware of this type of presentation by HTLV-1-associated lymphoma, especially in patients who came from endemic areas, even if not all clinical features are present and particularly with hypercalcemia and lytic bone lesions.
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http://dx.doi.org/10.1177/23247096211013235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114285PMC
May 2021

Insight Into Microbial Community Aerosols Associated With Electronic Waste Handling Facilities by Culture-Dependent and Culture-Independent Methods.

Front Public Health 2021 6;9:657784. Epub 2021 Apr 6.

Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.

Airborne microorganisms in the waste associated environments are more active and complex compared to other places. However, the diversity and structure of airborne bacteria in waste-associated environments are still not clearly understood. The purpose of this study was to assess airborne bacterial community in electronic waste dismantling site and a waste transfer station based on culture-dependent and culture-independent methods. A total of 229 isolates were obtained from four airborne sites collected from residential area, electronic industrial park, and office area in or near an electronic waste dismantling site and a waste transfer station in Southern China in the morning, afternoon, and evening. Most of the isolates were isolated from air for the first time and 14 potentially novel species were identified by Sanger sequencing. Bacterial communities in waste-associated bioaerosols were predominated by Proteobacteria and Bacteroidetes. Abundant genera (>1%) included Paracaedibacteraceae (uncultured EF667926), , Chitinophagaceae (uncultured FN428761), , and . One-third of the species in these genera were uncultured approximately. Differences community structure existed in airborne bacterial diversity among different sampling sites. These results showed that waste-associated environments have unique bacterial diversity. Further studies on such environments could provide new insights into bacterial community.
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http://dx.doi.org/10.3389/fpubh.2021.657784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055949PMC
May 2021

LncRNA XIST upregulates TRIM25 via negatively regulating miR-192 in hepatitis B virus-related hepatocellular carcinoma.

Mol Med 2021 04 15;27(1):41. Epub 2021 Apr 15.

Department of Interventional Radiology, Huaian Hospital of Huai'an City, No. 161 Zhenhuailou East Road, Huai'an City, 223200, Jiangsu Province, People's Republic of China.

Background: Long non-coding RNA (lncRNA) XIST has been implicated in the progression of a variety of tumor diseases. The purpose of this study was to explore the molecular role of lncRNA XIST in human hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).

Methods: The expression levels of lncRNA XIST, miR-192 and TRIM25 in HBV-related HCC tissues and HepG2.2.15 cells were detected by qRT-PCR. Biological information and luciferin gene reporter assay were performed to detect the interaction among lncRNA XIST, miR-192 and TRIM25. CCk-8 assay, wound healing assay and colony formation assay were conducted to detect the proliferation and migration ability of HepG2.2.15 cells.

Results: qRT-PCR results showed that the expression levels of lncRNA XIST were remarkably increased in HBV-related HCC tissues and HepG2.2.15 cells. In addition, miR-192 was a direct target gene of lncRNA XIST, and the expression of miR-192 and lncRNA XIST were negatively correlated. Moreover, overexpression of miR-192 observably inhibited the proliferation and migration of HCC cells, while overexpression of lncRNA XIST showed an opposite effect. Furthermore, TRIM25 was a direct target of miR-192, and lncRNA XIST could up-regulate the expression of TRIM25 by targeting miR-192.

Conclusion: LncRNA XIST could up-regulate the expression of TRIM25 by targeting and binding to miR-192, thus accelerating the occurrence and development of HCC.
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http://dx.doi.org/10.1186/s10020-021-00278-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050905PMC
April 2021

The Influence of Parental Knowledge and Basic Psychological Needs Satisfaction on Peer Victimization and Internet Gaming Disorder among Chinese Adolescents: A Mediated Moderation Model.

Int J Environ Res Public Health 2021 03 1;18(5). Epub 2021 Mar 1.

Department of Psychology and Research Center of Adolescent Psychology and Behavior, School of Education, Guangzhou University, Guangzhou 510006, China.

Ample evidence indicates that peer victimization is a crucial risk factor for adolescent internet gaming disorder (IGD); however, little is known about the mechanisms underlying this association. Based on the risk-buffering model and self-determination theory, this study tested whether parental knowledge moderated the relationship between peer victimization and adolescent IGD and whether this moderating effect was mediated by basic psychological needs satisfaction (BPNS). A sample of 3080 adolescents ( = 14.51; = 1.97) anonymously responded to a set of questionnaires. The results revealed that the positive association between peer victimization and adolescent IGD was stronger among adolescents with parents who had low-level parental knowledge than for those with high-level parental knowledge. Moreover, this moderating effect was mediated by BPNS. These findings highlight that parental knowledge is an important protective factor against IGD for adolescents who experience peer victimization and BPNS is one mechanism that explains how this effect works.
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http://dx.doi.org/10.3390/ijerph18052397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967736PMC
March 2021

Chemical Composition of Tomato Seed Flours, and Their Radical Scavenging, Anti-Inflammatory and Gut Microbiota Modulating Properties.

Molecules 2021 Mar 9;26(5). Epub 2021 Mar 9.

Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.

In the current study, the chemical composition and total phenolic content of tomato seed flours, along with potential health beneficial properties, including free radical scavenging capacities, anti-inflammatory capacities, and gut microbiota profile modulation, were examined using two different batches. Eight compounds were identified in the tomato seed flour, including malic acid, 2-hydroxyadipic acid, salicylic acid, naringin, -acetyl-tryptophan, quercetin-di--hexoside, kaempferol-di--hexoside, and azelaic acid. The total phenolic contents of tomato seed flour were 1.97-2.00 mg gallic acid equivalents/g. Oxygen radical absorbing capacities (ORAC), 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacities (DPPH), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical scavenging capacities (ABTS) were 86.32-88.57, 3.57-3.81, and 3.39-3.58 µmoles Trolox equivalents/g, respectively, on a per flour dry weight basis. The mRNA expression of the pro-inflammatory markers, interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α), were dose-dependently suppressed by tomato seed flour extracts. The extracts altered five of the eight bacterial phyla and genera evaluated. The results may provide some scientific support for the use of tomato seed flour as value-added food ingredients.
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http://dx.doi.org/10.3390/molecules26051478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7963151PMC
March 2021

Cybervictimization and Adolescent Internet Addiction: A Moderated Mediation Model.

Int J Environ Res Public Health 2021 03 2;18(5). Epub 2021 Mar 2.

School of Psychology and Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, China.

Previous research indicates that cybervictimization can lead to adolescent Internet addiction; however, there is a gap in the knowledge about the mediating and moderating variables facilitating this relationship. This study examines the role of rejection sensitivity as a mediator in this relationship and the role of parent-adolescent communication as a moderator for this mediation effect among Chinese adolescents. Participants were 1006 adolescents (M = 13.16 years, SD = 0.67), who completed the questionnaires anonymously. The questionnaires assessed the four variables of interest. Descriptive statistics and structural equation modeling were used for data analysis. The results show that the positive association between cybervictimization and adolescent Internet addiction is mediated by rejection sensitivity. Moreover, this indirect effect is stronger for adolescents with low parent-adolescent communication than for those with high parent-adolescent communication.
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http://dx.doi.org/10.3390/ijerph18052427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967556PMC
March 2021
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