Publications by authors named "Pawan Kumar"

542 Publications

Light-matter coupling in large-area van der Waals superlattices.

Nat Nanotechnol 2021 Dec 2. Epub 2021 Dec 2.

Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, PA, USA.

Two-dimensional (2D) crystals have renewed opportunities in design and assembly of artificial lattices without the constraints of epitaxy. However, the lack of thickness control in exfoliated van der Waals (vdW) layers prevents realization of repeat units with high fidelity. Recent availability of uniform, wafer-scale samples permits engineering of both electronic and optical dispersions in stacks of disparate 2D layers with multiple repeating units. Here we present optical dispersion engineering in a superlattice structure comprising alternating layers of 2D excitonic chalcogenides and dielectric insulators. By carefully designing the unit cell parameters, we demonstrate greater than 90% narrow band absorption in less than 4 nm of active layer excitonic absorber medium at room temperature, concurrently with enhanced photoluminescence in square-centimetre samples. These superlattices show evidence of strong light-matter coupling and exciton-polariton formation with geometry-tuneable coupling constants. Our results demonstrate proof of concept structures with engineered optical properties and pave the way for a broad class of scalable, designer optical metamaterials from atomically thin layers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41565-021-01023-xDOI Listing
December 2021

A fluorene based probe: Synthesis and "turn-on" water sensitivity of the in-situ formed Cu complex: Application in bio-imaging.

Anal Chim Acta 2022 Jan 26;1189:339211. Epub 2021 Oct 26.

Department of Chemistry, UGC Centre of Advanced Study, Guru Nanak Dev University, Amritsar, 143005, India. Electronic address:

A new fluorene based probe (FTH) has been evaluated for its photo-physical properties in solution as well as in the aggregated state/viscous environment. Addition of a poor solvent (water) to the solution of the probe in a good (acetonitrile) solvent significantly enhanced the otherwise weak emission due to aggregation induced emission (AIE). The emission enhancement is also related to the increase in viscosity of the solution, leading to the restricted intramolecular rotation of the peripheral (phenyl) groups. Interestingly, the emission behaviour of the non-emissive in-situ formed Cu complex is drastically modulated in the presence of water. The solution of the putative Cu complex of the probe turns highly emissive (yellow colour) upon addition of a small fraction of water (up to 7.6 wt %), but the yellow emission diminishes upon increasing higher water fraction. We propose that the initially formed Cu complex undergoes hydrolysis in the presence of higher water content releasing the free amine possessing the diaryl amino rotors thus rendering the solution non-emissive. Thus the current probe being reported herein discloses its potential to generate trace water sensitive turn-on Cu complex. Additionally, the bio-imaging potential of FTH for live cancer cells and its sensitivity towards intracellular presence of Cu ions has been demonstrated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2021.339211DOI Listing
January 2022

Correction to: Whole genome sequences of two clinical isolates from India emerging as threats during therapeutic treatment of dermatophytosis.

3 Biotech 2021 Nov 30;11(11):482. Epub 2021 Oct 30.

CSIR-Institute of Genomics and Integrative Biology, New Delhi, 110025 India.

[This corrects the article DOI: 10.1007/s13205-021-02950-1.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13205-021-03005-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557218PMC
November 2021

Silver nanoparticle based multifunctional approach for combating COVID-19.

Sens Int 2021 2;2:100101. Epub 2021 Jun 2.

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science (CVSc), Warangal - 506166, PVNRTVU, Telangana, India.

COVID-19 is a highly contagious and widespread disease that has strained the global healthcare system to the hilt. Silver nanoparticles (AgNPs) are well known for their potent antimicrobial, antiviral, immunomodulatory and biosensing properties. AgNPs have been found to be potential antiviral agent that act against many deadly viruses and is presumed to be effective against COVID-19. AgNPs can generate free radicals and reactive oxygen species (ROS) leading to apoptosis mediated cell death thereby inhibiting viral infection. The shape and size of AgNPs play an important role in its biomedical applications as alterations may result in variable biological interaction and activity. Herein, we propose that AgNPs can be utilized for effective management of the ongoing COVID-19 pandemic by highlighting the current status of AgNPs in the fight against COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sintl.2021.100101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169222PMC
June 2021

Changing microbiological profile and antimicrobial susceptibility of the isolates obtained from patients with infective endocarditis - The time to relook into the therapeutic guidelines.

Indian Heart J 2021 Nov-Dec;73(6):704-710. Epub 2021 Nov 1.

Department of Cardiology, Dayanand Medical College and Hospital, Unit Hero DMC Heart Institute, Ludhiana, India. Electronic address:

The microbiological profile, associated risk factors and demographic characteristics of patients with IE has changed in the recent times. In the present study, the antibiotic susceptibility profile of 66 isolates (40 from IDU and 26 from non IDU) recovered over a period of three years from the patients with definitive diagnosis of IE along with their absolute minimum inhibitory concentrations (MIC-μg/ml) was determined as per CLSI, 2017 guidelines. Staphylococcus aureus was found to be the predominant pathogen associated with IE out of which 90.2% isolates were MRSA, although none of the isolates were found resistant to vancomycin, teicoplanin, daptomycin and linezolid. Pseudomonas aeruginosa isolates were 100% susceptible to carbapenams, however variable resistance was observed against other antimicrobials. All Enterococci were found to be 100% susceptible to linezolid and daptomycin, whereas vancomycin resistant enterococci phenotype was observed in 25% of the Enterococcal isolates. A noticeable difference in the antimicrobial susceptibility profile and their MICs were observed in the present study, as compared to published literature across the globe and within the country. However, no statistically significant difference (λ test, p > 0.01)in the AST pattern of isolates from IDU vs. Non IDU was observed. After reviewing the local antibiogram it seems that we need to have our own regional guidelines, which may partially replace the currently prevailing AHA/ESC guidelines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ihj.2021.10.013DOI Listing
November 2021

Chronic Uveitis in Children.

Indian J Pediatr 2021 Nov 3. Epub 2021 Nov 3.

Pediatric Allergy Immunology Unit, Department of Pediatrics, Postgraduate Institute of Medical Education and Research, Chandigrah, 160012, India.

Objective: To describe the experience of managing chronic childhood uveitis from a tertiary care center in India.

Methods: All children diagnosed as chronic uveitis between January 2005 and December 2012 and on follow-up in Pediatric Rheumatology Clinic and Uveitis Clinic, were eligible for enrollment. Information regarding demographics, type of uveitis, treatment, complications, and surgical procedures was obtained from clinic records. All the enrolled patients were assessed for outcome prospectively and underwent a detailed ophthalmological examination to document visual acuity, refraction, intraocular pressure (IOP), slit lamp examination, fundus examination, and vitreous haze findings.

Results: Sixty-seven children with chronic uveitis were enrolled in the study. Anterior uveitis was the commonest type seen in 45 children. Juvenile idiopathic arthritis (JIA) was the commonest known etiology and diagnosis of uveitis was made during routine screening in a majority of the JIA patients. No cause could be identified in 43% patients. After a mean follow-up period of 3.95 ± 1.99 y, only 16% eyes were in remission and off therapy. Prolonged oral glucocorticoids were required, besides other immunosuppressants, to control inflammation in 50% patients. Ocular complications were seen in 87% cases with posterior synechiae, band-shaped keratopathy and cataracts being the commonest complications.

Conclusions: Among patients with chronic uveitis, 43% had no identifiable cause. JIA was the commonest known cause. Significant ocular complications were common. Even after a mean follow-up of 3.95 ± 1.99 y, a vast majority continued to need immunosuppression for control of disease activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12098-021-03884-5DOI Listing
November 2021

Genetic diversity and population structure analysis for morphological traits in upland cotton (Gossypium hirsutum L.).

J Appl Genet 2021 Oct 31. Epub 2021 Oct 31.

Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, 125004, India.

A total of 96 different genotypes of upland cotton (Gossypium hirsutum) were selected from the breeding material and germplasm available at CCS HAU, India, to find the novel marker-trait associations for morphological traits used for registration of variety in upland cotton. Twenty-three morphological traits of the selected genotypes were recorded in field trials conducted in two replication of randomized block design during Kharif 2018 and 2019. A total of 11 traits showed sufficient variations in the screened germplasm and the same were further used for association mapping. A total of 168 SSRs were used for genotyping, of which 97 SSRs showed polymorphism amplifying 293 different alleles with an average of 3.02 alleles per SSR. Clustering, principal component analysis, and population structure analysis advocated that the current germplasm panel has enough diversity to be considered for association mapping. A total of 20 significant marker-trait associations were identified by the mixed linear model (MLM) and compressed mixed linear model (CMLM), of which 15 were common to both models, hence considered as promising associations. To the best of our knowledge, it is a first attempt to identify the linked markers in relation to morphological traits for the cotton crop. Results of the present study will be highly useful in speeding up variety registration programmes of upland cotton complementing to Distinctiveness, Uniformity, and Stability (DUS) testing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13353-021-00667-8DOI Listing
October 2021

Safety and Efficacy of Furosap®, a Patented Seed Extract, in Boosting Testosterone Level, Reproductive Health and Mood Alleviation in Male Volunteers.

J Am Coll Nutr 2021 Oct 25:1-9. Epub 2021 Oct 25.

College of Pharmacy and Health Sciences, Texas Southern University, Houston, Texas, USA.

Background: The medicinal herb fenugreek () seeds, fortified with dietary fibers and furostanolic saponins including protodioscin, have demonstrated a significant contribution to human health. In our laboratories, Furosap, a patented 20% protodioscin-enriched extract was developed from fenugreek seeds.

Objective: In an open-label, one-arm, single-center longitudinal study, we examined the safety and efficacy of Furosap on free and total testosterone levels, fasting blood sugar, blood pressure, sperm count, motility and morphology, dihydroepiandrosterone sulfate (DHEA-S), sexual health, reflex erection, mood alleviation, mental alertness, and total blood chemistry analyses over a period of 12 weeks in healthy male volunteers.

Methods: Institutional Ethics Committee approvals and Clinicaltrials.gov registration were obtained. Effect of Furosap (500 mg/day) was examined of free and total testosterone levels, sperm count, motility and morphology, sexual health, mood and mental alertness, and total blood chemistry analyses in 100 healthy volunteers (age 35-60 Y) over a period of 12 consecutive weeks.

Results: No changes were observed in body weight and BMI. Both systolic and diastolic blood pressure, and DHEA levels significantly decreased. Free and bound testosterone levels improved significantly at 12 weeks of treatment. Sperm motility significantly increased at 8- and 12-weeks of treatment, while abnormal sperm morphology significantly decreased at 12-weeks of treatment. Mental alertness, mood, and reflex erection score significantly alleviated. An age-induced increasing effect was observed. Furthermore, cardiovascular health and libido significantly improved. Blood chemistry analyses exhibited broad spectrum safety. A decreasing trend was observed in total cholesterol, triglycerides, and VLDL levels, while an increasing trend was observed in HDL level at 12 weeks of treatment. LDL level decreased significantly at 12-weeks of treatment. No adverse events were observed.

Conclusion: Results demonstrate that Furosap is safe and effective in improving testosterone levels, cardiovascular health, healthy sperm profile, mental alertness in human male volunteers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/07315724.2021.1978348DOI Listing
October 2021

Hierarchical Self-Assembly of Metal-Ion-Modulated Chitosan Tubules.

Langmuir 2021 Nov 21;37(43):12690-12696. Epub 2021 Oct 21.

Department of Physical Chemistry and Materials Science, University of Szeged, Rerrich Béla tér 1., Szeged H-6720, Hungary.

Soft materials such as gels or biological tissues can develop self-assembly under chemo-mechanical forces. Here, we report the instantaneous formation of soft tubular structures with a two-level hierarchy by injecting a mixture of inorganic salt and chitosan (CS) solution from below into a reactor filled with alkaline solution. Folding and wrinkling instabilities occur on the originally smooth surface controlled by the salt composition and concentration. Liesegang-like precipitation patterns develop on the outer surface on a μm length scale in the presence of calcium chloride, while the precipitate particles are distributed evenly in the bulk as corroborated by X-ray μ-CT. On the other hand, barium hydroxide precipitates out only in the thin outer layer of the CS tubule when barium chloride is introduced into the CS solution. Independent of the concentration of the weakly interacting salt, an electric potential gradient across the CS membrane develops, which vanishes when the pH difference between the two sides of the membrane diminishes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c02097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567419PMC
November 2021

Diagnostic Accuracy of FIB-4 and FIB-5 Scores as Compared to Fibroscan for Assessment of Liver Fibrosis in Patients With Non-Alcoholic Fatty Liver Disease.

Cureus 2021 Aug 31;13(8):e17622. Epub 2021 Aug 31.

Biochemistry, All India Institute of Medical Sciences, Patna, Patna, IND.

Introduction Limited access/exorbitant cost of fibroscan and the associated risks with biopsy to assess fibrosis in non-alcoholic fatty liver disease (NAFLD) patients has made exigent demand of serum-based fibrosis scores to be validated for their accuracy and efficacy. The objective of the study was to compare the accuracy of FIB-4 (fibrosis-4) and FIB-5 (fibrofast) scores to rule out advanced fibrosis in NAFLD patients. Methods A total of 145 patients were categorized as group I with mild/moderate fibrosis (MF) comprising of F0 to F2 and group II with advanced fibrosis (AF) comprising of F3 and F4 based on fibroscan kPa (kilopascal) score. Results Group II had significantly higher alanine transaminase (ALT), aspartate transaminase (AST), haemoglobin % (Hb %), bilirubin and alkaline phosphatase (ALP) values and significantly lower platelet count and albumin as compared to group I. The FIB-4 score was significantly higher in group II [1.8 (1.1 - 4.7)], as compared with group I [0.98 (0.63 - 1.67)], p-value = 0.0001. FIB-5 score of group II [-6.4 (-8.8 - 3.4)] was significantly lower as compared with group I [-4.8 (-6.8 - 2.0)], p-value = 0.003. FIB-4 and FIB-5 had area under receiver operator characteristic (AUROC) curve of 0.712 and 0.655, respectively. FIB-4 at cut-off of <2.02 had a negative predictive value (NPV) of 90.7%. FIB-5 at a cut-off of <-7.11 has an NPV of 94.1% and at a cut-off of <-3.24 had an NPV of 88.9%. Conclusion We concluded that both FIB-4 and FIB-5 can be used to rule out advanced fibrosis in NAFLD patients in a resource-limited and indigent setting as both the scores have NPV greater than 90%.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7759/cureus.17622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486360PMC
August 2021

Development of a Novel Pharmacophore Model Guided by the Ensemble of Waters and Small Molecule Fragments Bound to SARS-CoV-2 Main Protease.

Mol Inform 2021 Oct 11:e2100178. Epub 2021 Oct 11.

National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.

Recent fragment-based drug design efforts have generated huge amounts of information on water and small molecule fragment binding sites on SARS-CoV-2 M and preference of the sites for various types of chemical moieties. However, this information has not been effectively utilized to develop automated tools for in silico drug discovery which are routinely used for screening large compound libraries. Utilization of this information in the development of pharmacophore models can help in bridging this gap. In this study, information on water and small molecule fragments bound to M has been utilized to develop a novel Water Pharmacophore (Waterphore) model. The Waterphore model can also implicitly represent the conformational flexibilities of binding pockets in terms of pharmacophore features. The Waterphore model derived from 173 apo- or small molecule fragment-bound structures of M has been validated by using a dataset of 68 known bioactive inhibitors and 78 crystal structure bound inhibitors of SARS-CoV-2 M . It is encouraging to note that, even though no inhibitor data has been used in developing the Waterphore model, it could successfully identify the known inhibitors from a library of decoys with a ROC-AUC of 0.81 and active hit rate (AHR) of 70 %. The Waterphore model is also general enough for potential applications for other drug targets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/minf.202100178DOI Listing
October 2021

Zinc phthalocyanine conjugated cellulose nanocrystals for memory device applications.

Nanotechnology 2021 Nov 8;33(5). Epub 2021 Nov 8.

Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 1H9, Canada.

We present the electrical properties of zinc phthalocyanine covalently conjugated to cellulose nanocrystals ([email protected]). Thin films of [email protected] sandwiched between two gold electrodes showed pronounced hysteresis in their current-voltage characteristics. The layered metal-organic-metal sandwich devices exhibit distinct high and low conductive states when bias is applied, which can be used to store information. Density functional theory results confirmed wave function overlap between CNC and ZnPc in [email protected], and helped visualize the lowest (lowest unoccupied molecular orbital) and highest molecular orbitals (highest occupied molecular orbital) in [email protected] These results pave the way forward for all-organic electronic devices based on low cost, earth abundant CNCs and metallophthalocyanines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac2e78DOI Listing
November 2021

Photocatalytic Mechanism Control and Study of Carrier Dynamics in [email protected] Core-Shell Nanowires.

ACS Appl Mater Interfaces 2021 Oct 5;13(40):47418-47439. Epub 2021 Oct 5.

Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada.

We present a potential solution to the problem of extraction of photogenerated holes from CdS nanocrystals and nanowires. The nanosheet form of CN is a low-band-gap ( = 2.03 eV), azo-linked graphenic carbon nitride framework formed by the polymerization of melem hydrazine (MHP). CN nanosheets were either wrapped around CdS nanorods (NRs) following the synthesis of pristine chalcogenide or intercalated among them by an synthesis protocol to form two kinds of heterostructures, CdS-MHP and CdS-MHPINS, respectively. CdS-MHP improved the photocatalytic degradation rate of 4-nitrophenol by nearly an order of magnitude in comparison to bare CdS NRs. CdS-MHP also enhanced the sunlight-driven photocatalytic activity of bare CdS NWs for the decolorization of rhodamine B (RhB) by a remarkable 300% through the improved extraction and utilization of photogenerated holes due to surface passivation. More interestingly, CdS-MHP provided reaction pathway control over RhB degradation. In the absence of scavengers, CdS-MHP degraded RhB through the N-deethylation pathway. When either hole scavenger or electron scavenger was added to the RhB solution, the photocatalytic activity of CdS-MHP remained mostly unchanged, while the degradation mechanism shifted to the chromophore cleavage (cycloreversion) pathway. We investigated the optoelectronic properties of CdS-CN heterojunctions using density functional theory (DFT) simulations, finite difference time domain (FDTD) simulations, time-resolved terahertz spectroscopy (TRTS), and photoconductivity measurements. TRTS indicated high carrier mobilities >450 cm V s and carrier relaxation times >60 ps for CdS-MHP, while CdS-MHPINS exhibited much lower mobilities <150 cm V s and short carrier relaxation times <20 ps. Hysteresis in the photoconductive characteristics of CdS NWs disappeared in CdS-MHP, confirming surface passivation. Dispersion-corrected DFT simulations indicated a delocalized HOMO and a LUMO localized on CN in CdS-MHP. CN, with its extended π-conjugation and low band gap, can function as a shuttle to extract carriers and excitons in nanostructured heterojunctions, and enhance performance in optoelectronic devices. Our results demonstrate how carrier dynamics in core-shell heterostructures can be manipulated to achieve control over the reaction mechanism in photocatalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c08550DOI Listing
October 2021

Polyethylene Hydrogenolysis at Mild Conditions over Ruthenium on Tungstated Zirconia.

JACS Au 2021 Sep 30;1(9):1422-1434. Epub 2021 Aug 30.

Center for Plastics Innovation, University of Delaware, 221 Academy Street, Newark, Delaware 19716, United States.

Plastics waste has become a major environmental threat, with polyethylene being one of the most produced and hardest to recycle plastics. Hydrogenolysis is potentially the most viable catalytic technology for recycling. Ruthenium (Ru) is one of the most active hydrogenolysis catalysts but yields too much methane. Here we introduce ruthenium supported on tungstated zirconia (Ru-WZr) for hydrogenolysis of low-density polyethylene (LDPE). We show that the Ru-WZr catalysts suppress methane formation and produce a product distribution in the diesel and wax/lubricant base-oil range unattainable by Ru-Zr and other Ru-supported catalysts. Importantly, the enhanced performance is showcased for real-world, single-use LDPE consumables. Reactivity studies combined with characterization and density functional theory calculations reveal that highly dispersed (WO ) clusters store H as surface hydroxyls by spillover. We correlate this hydrogen storage mechanism with hydrogenation and desorption of long alkyl intermediates that would otherwise undergo further C-C scission to produce methane.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacsau.1c00200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479762PMC
September 2021

Mechanical, Electrical, and Biological Properties of Mechanochemically Processed Hydroxyapatite Ceramics.

Nanomaterials (Basel) 2021 Aug 28;11(9). Epub 2021 Aug 28.

Department of Physics and Astronomy, NIT Rourkela, Rourkela 769008, India.

The effect of the sintering temperature on densification and the resultant mechanical, electrical, and biological properties of mechanochemically processed hydroxyapatite (HAp) samples was investigated. HAp samples were sintered at 1200, 1250, and 1300 °C for 4 h, respectively. HAp samples sintered at 1250 °C showed better mechanical properties, which was attributed to their smaller grain size compared to HAp samples at higher sintering temperatures. The nearly identical value of the dielectric constant (ε) and better cell proliferation was exhibited by HAp samples sintered at 1250 and 1300 °C, respectively. At ~210 °C, in all the samples sintered at different temperatures, a dielectric anomaly was obtained, which was attributed to the phase transition temperature of the HAp system. Dielectric properties near the phase transition temperature showed a dielectric relaxation-type of behavior, which was attributed to the re-orientational motion of OH ions in the HAp system. Higher cell proliferation and viability were exhibited by the HAp1300 samples, whereas comparatively equivalent cell growth and higher mechanical strength were observed in the HAp1250 samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano11092216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466523PMC
August 2021

Tracing Viral Transmission and Evolution of Bovine Leukemia Virus through Long Read Oxford Nanopore Sequencing of the Proviral Genome.

Pathogens 2021 Sep 14;10(9). Epub 2021 Sep 14.

CentralStar Cooperative, Lansing, MI 48910, USA.

Bovine leukemia virus (BLV) causes Enzootic Bovine Leukosis (EBL), a persistent life-long disease resulting in immune dysfunction and shortened lifespan in infected cattle, severely impacting the profitability of the US dairy industry. Our group has found that 94% of dairy farms in the United States are infected with BLV with an average in-herd prevalence of 46%. This is partly due to the lack of clinical presentation during the early stages of primary infection and the elusive nature of BLV transmission. This study sought to validate a near-complete genomic sequencing approach for reliability and accuracy before determining its efficacy in characterizing the sequence identity of BLV proviral genomes collected from a pilot study made up of 14 animals from one commercial dairy herd. These BLV-infected animals were comprised of seven adult dam/daughter pairs that tested positive by ELISA and qPCR. The results demonstrate sequence identity or divergence of the BLV genome from the same samples tested in two independent laboratories, suggesting both vertical and horizontal transmission in this dairy herd. This study supports the use of Oxford Nanopore sequencing for the identification of viral SNPs that can be used for retrospective genetic contact tracing of BLV transmission.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/pathogens10091191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470207PMC
September 2021

Estimating the Volume of Equine Rabies Immunoglobulin (eRIG) Required for Local Infiltration in Soft Tissue Animal Bites in Children Using a Wound Size-Based Approach.

J Trop Pediatr 2021 08;67(4)

Department of Community Medicine, University College of Medical Sciences & Guru Teg Bahadur Hospital, Delhi 110095, India.

Introduction: Post-exposure prophylaxis using rabies immunoglobulin (RIG) is universal recommendation for category III exposures. This study was conducted to determine quantity of RIG used to infiltrate animal bite wounds in relation to longest dimension and area of wound.

Methods: Children aged 2-18 years reporting within 7 days of category III animal bite were included. eRIG (300 IU/ml) was used in all subjects. A predesigned proforma was filled for clinical and epidemiological details of subjects. Maximum length and width of wounds was measured by using non-stretchable measuring tape. RIG was infiltrated in wounds as per WHO recommendations and volume infiltrated was noted.

Results And Conclusion: A total of 100 subjects were enrolled. The mean (±SD) volume of RIG infiltrated in wounds per unit length was 0.75 (±0.21) ml/cm and per unit area was 3.18 (±1.75) ml/cm2. Regression equations were calculated. Proposed equations y = 0.6x + 0.3, where y is the volume of RIG (ml) and x is the length of the wound (cm) and y = 0.9x + 1.1, where y is the volume of RIG (ml) and x is the area of the wound (cm2) can prove to be a useful tool in RIG volume calculation for primary health care providers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/tropej/fmab082DOI Listing
August 2021

Modified plant architecture integrated with liquid fertilizers improves fruit productivity and quality of tomato in North West Himalaya, India.

Sci Rep 2021 09 20;11(1):18664. Epub 2021 Sep 20.

ICAR-Indian Institute of Soil and Water Conservation, 218 Kaulagarh Road, Dehradun, 248195, Uttarakhand, India.

India produces around 19.0 million tonnes of tomatoes annually, which is insufficient to meet the ever-increasing demand. A big gap of tomato productivity (72.14 t ha) between India (24.66 t ha) and the USA (96.8 t ha) exist, which can be bridged by integrating trellis system of shoot training, shoot pruning, liquid fertilizers, farmyard manure, and mulching technologies. Therefore, the present experiment was conducted on tomato (cv. Himsona) during 2019-2020 at farmers' fields to improve tomato productivity and quality. There were five treatments laid in a randomized block design (RBD) with three replications; T [Farmer practice on the flatbed with RDF @ N:P:K + FYM @6.0 t ha without mulch], T [T + Polythene mulch (50 microns)], T [Tomato plants grown on the raised bed with polythene mulch + FYM @ 8.0 t ha + Single shoot trellis system + Side shoot pruning + Liquid Fertilizer (LF-N:P:K) @ 2.0 g l for vegetative growth + Liquid Fertilizer (LF-N: P: K) @ 1.5 g l for improving fruit quality], T [Tomato plants grown on the raised bed with polythene mulch + FYM @ 8.0 t ha + Single shoot trellis system + Side shoot pruning + LF @ 4.0 g l + LF @ 3.0 g l], and T [Tomato plants grown on the raised bed with polythene mulch + FYM @ 10.0 t ha + Single shoot trellis system + Side shoot pruning + LF @ 6.0 g l + LF @ 4.5 g l]. The results revealed that tomato plant grown on the raised beds with polythene mulch, shoot pruning, trellising, liquid fertilizers, and farmyard manure (i.e., T) recorded higher shoot length, dry matter content, and tomato productivity by 20.75-141.21, 18.79-169.4, and 18.89-160.87% as compared to T-T treatments, respectively. The T treatment also recorded the highest water productivity (28.39 kg m), improved fruit qualities, net return (10,751 USD ha), benefit-cost ratio (3.08), microbial population, and enzymatic activities as compared to other treatments. The ranking and hierarchical clustering of treatments confirmed the superiority of the T treatment over all other treatments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-98209-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452768PMC
September 2021

Cutting Edge: Intestinal IL-17A Receptor Signaling Specifically Regulates High-Fat Diet-Mediated, Microbiota-Driven Metabolic Disorders.

J Immunol 2021 10 20;207(8):1959-1963. Epub 2021 Sep 20.

Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY

Previous studies indicate that IL-17A plays an important role in mediating the intestinal microbiota and systemic metabolic functions. However, it is not known where IL-17RA signaling occurs to mediate these effects. To investigate this question, we used intestinal epithelial-specific ( ) and liver-specific ( ) IL-17RA knockout mice as well as littermate control mice. Our results indicate that intestinal IL-17RA signaling helps mediate systemic metabolic functions upon exposure to prolonged high-fat diet. mice display impaired glucose metabolism, altered hormone and adipokine levels, increased visceral adiposity, and greater hepatic lipid deposition when compared with their littermate controls. We show that IL-17RA-driven changes in microbiota composition are responsible for regulating systemic glucose metabolism. Altogether, our data elucidate the importance of intestinal IL-17RA signaling in regulating high-fat diet-mediated systemic glucose and lipid metabolism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4049/jimmunol.2000986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556717PMC
October 2021

Dietary suppression of MHC class II expression in intestinal epithelial cells enhances intestinal tumorigenesis.

Cell Stem Cell 2021 11 15;28(11):1922-1935.e5. Epub 2021 Sep 15.

The David H. Koch Institute for Integrative Cancer Research at MIT, Department of Biology, MIT, Cambridge, MA 02139, USA; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02140, USA.

Little is known about how interactions of diet, intestinal stem cells (ISCs), and immune cells affect early-stage intestinal tumorigenesis. We show that a high-fat diet (HFD) reduces the expression of the major histocompatibility complex class II (MHC class II) genes in intestinal epithelial cells, including ISCs. This decline in epithelial MHC class II expression in a HFD correlates with reduced intestinal microbiome diversity. Microbial community transfer experiments suggest that epithelial MHC class II expression is regulated by intestinal flora. Mechanistically, pattern recognition receptor (PRR) and interferon-gamma (IFNγ) signaling regulates epithelial MHC class II expression. MHC class II-negative (MHC-II-) ISCs exhibit greater tumor-initiating capacity than their MHC class II-positive (MHC-II+) counterparts upon loss of the tumor suppressor Apc coupled with a HFD, suggesting a role for epithelial MHC class II-mediated immune surveillance in suppressing tumorigenesis. ISC-specific genetic ablation of MHC class II increases tumor burden cell autonomously. Thus, HFD perturbs a microbiome-stem cell-immune cell interaction that contributes to tumor initiation in the intestine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.stem.2021.08.007DOI Listing
November 2021

Small circular interfering RNAs (sciRNAs) as a potent therapeutic platform for gene-silencing.

Nucleic Acids Res 2021 Oct;49(18):10250-10264

Alnylam Pharmaceuticals, Inc., Cambridge, MA 02142, USA.

In order to achieve efficient therapeutic post-transcriptional gene-silencing mediated by the RNA interference (RNAi) pathway, small interfering RNAs (siRNAs) must be chemically modified. Several supra-RNA structures, with the potential to stabilize siRNAs metabolically have been evaluated for their ability to induce gene silencing, but all have limitations or have not been explored in therapeutically relevant contexts. Covalently closed circular RNA transcripts are prevalent in eukaryotes and have potential as biomarkers and disease targets, and circular RNA mimics are being explored for use as therapies. Here we report the synthesis and evaluation of small circular interfering RNAs (sciRNAs). To synthesize sciRNAs, a sense strand functionalized with the trivalent N-acetylgalactosamine (GalNAc) ligand and cyclized using 'click' chemistry was annealed to an antisense strand. This strategy was used for synthesis of small circles, but could also be used for synthesis of larger circular RNA mimics. We evaluated various sciRNA designs in vitro and in vivo. We observed improved metabolic stability of the sense strand upon circularization and off-target effects were eliminated. The 5'-(E)-vinylphosphonate modification of the antisense strand resulted in GalNAc-sciRNAs that are potent in vivo at therapeutically relevant doses. Physicochemical studies and NMR-based structural analysis, together with molecular modeling studies, shed light on the interactions of this novel class of siRNAs, which have a partial duplex character, with the RNAi machinery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkab724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501968PMC
October 2021

Predictors of Pain Severity and its Impact on Quality of Life in Patients with Parkinson's Disease.

Neurol India 2021 Jul-Aug;69(4):979-983

Department of Neurology, Lady Hardinge Medical College, New Delhi, India.

Background: Pain is a common and distressing symptom of Parkinson's disease (PD). The relation of pain, its predictors, and its impact on quality of life (QoL) in PD has not been studied in Indian PD patients.

Objective: To assess the predictors of pain and investigate its impact on QoL among Indian PD patients.

Methodology: We conducted a cross-sectional study on 100 PD patients. The cases were diagnosed according to the UK brain bank criteria. Unified PD Rating Scale (UPDRS) parts III, V, and VI were employed to assess the severity of the disease. King's Parkinson Disease Pain Scale (KPPS) and PD questionnaire-8 (PDQ-8) were used to evaluate pain and QoL, respectively.

Results: Prevalence of different pain types in patients with PD was 70%, mainly including musculoskeletal (53%), fluctuation-related (35%), and nocturnal pain (27%). Subjects with pain developed PD symptoms at a younger age and had a longer duration of the disease. A positive correlation was found between KPPS scores and UPDRS parts III and V, while a negative correlation was observed with UPDRS part VI. Pain in PD subjects had a significant impact on the QoL.

Conclusions: Most of the PD patients suffered some form of pain with significant correlations with motor disability and poor QoL. Predictors of pain severity among PD patients included a longer disease duration, younger age of disease onset, and a higher levodopa equivalent daily dose (LEDD).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/0028-3886.325323DOI Listing
September 2021

A bis-pyrene chalcone based fluorescent material for ratiometric sensing of hydrazine: An acid/base molecular switch and solid-state emitter.

Anal Chim Acta 2021 Sep 29;1178:338807. Epub 2021 Jun 29.

Department of Chemistry, UGC Centre of Advanced Study, Guru Nanak Dev University, Amritsar, 143005, India. Electronic address:

In this work, we have designed and synthesized a new fluorescent molecular probe, DPY comprising of pyrene-diacetylpyridine conjugate, which was found to be sensitive to hydrazine as well as protonation. DPY is characterised by a strong emission both in solution (λ = 530 nm) as well as in solid state (λ = 610 nm), attributed to intramolecular charge-transfer. The probe responds to hydrazine with a ratiometric fluorescence emission change from yellow to blue, due to chalcone cyclisation reaction of α, β-unsaturated carbonyl group resulting in the pyrazoline compound, DPY-Hy, imparting a strong greenish-blue emission in solution. Further, the strong fluorescence emission of DPY in powder and thin film was quenched upon exposure to TFA, and revived upon exposure to TEA. For developing on-site detection protocol, when DPY was drop-casted on nonfluorescent silica plate a vivid naked-eye colour change from orange-red to dark blue was realized. Interestingly, in the aggregated state, DPY exhibited a broad range emission from green to orange in a mixed solvent system of THF:HO. A plausible explanation of the photophysical events is substantiated with theoretical calculations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2021.338807DOI Listing
September 2021

Harvesting Hot Holes in Plasmon-Coupled Ultrathin Photoanodes for High-Performance Photoelectrochemical Water Splitting.

ACS Appl Mater Interfaces 2021 Sep 3;13(36):42741-42752. Epub 2021 Sep 3.

Department of Electrical and Computer Engineering, University of Alberta, 9211-116 Street, Edmonton, Alberta T6G 1H9, Canada.

The harvesting of hot carriers produced by plasmon decay to generate electricity or drive a chemical reaction enables the reduction of the thermalization losses associated with supra-band gap photons in semiconductor photoelectrochemical (PEC) cells. Through the broadband harvesting of light, hot-carrier PEC devices also produce a sensitizing effect in heterojunctions with wide-band gap metal oxide semiconductors possessing good photostability and catalytic activity but poor absorption of visible wavelength photons. There are several reports of hot electrons in Au injected over the Schottky barrier into crystalline TiO and subsequently utilized to drive a chemical reaction but very few reports of hot hole harvesting. In this work, we demonstrate the efficient harvesting of hot holes in Au nanoparticles (Au NPs) covered with a thin layer of amorphous TiO (a-TiO). Under AM1.5G 1 sun illumination, photoanodes consisting of a single layer of ∼50 nm diameter Au NPs coated with a 10 nm shell of a-TiO ([email protected]) generated 2.5 mA cm of photocurrent in 1 M KOH under 0.6 V external bias, rising to 3.7 mA cm in the presence of a hole scavenger (methanol). The quantum yield for hot-carrier-mediated photocurrent generation was estimated to be close to unity for high-energy photons (λ < 420 nm). [email protected] photoelectrodes produced a small positive photocurrent of 0.1 mA cm even at a bias of -0.6 V indicating extraction of hot holes even at a strong negative bias. These results together with density functional theory modeling and scanning Kelvin probe force microscope data indicate fast injection of hot holes from Au NPs into a-TiO and light harvesting performed near-exclusively by Au NPs. For comparison, Au NPs coated with a 10 nm shell of AlO ([email protected]) generated 0.02 mA cm of photocurrent in 1 M KOH under 0.6 V external bias. These results underscore the critical role played by a-TiO in the extraction of holes in [email protected] photoanodes, which is not replicated by an ordinary dielectric shell. It is also demonstrated here that an ultrathin photoanode (<100 nm in maximum thickness) can efficiently drive sunlight-driven water splitting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c10698DOI Listing
September 2021

Whole genome sequences of two clinical isolates from India emerging as threats during therapeutic treatment of dermatophytosis.

3 Biotech 2021 Sep 9;11(9):402. Epub 2021 Aug 9.

CSIR-Institute of Genomics and Integrative Biology, New Delhi, 110025 India.

In the current study, we report the genome sequence of two different clinical isolates from India, UCMS-IGIB-CI12 and UCMS-IGIB-CI14. The resulting genome assembly achieved a 143-fold coverage in 824 contigs for UCMS-IGIB-CI12 and a 136-fold coverage in 904 contigs for UCMS-IGIB-CI14. Both the clinical isolates contain a c.1342G>A mutation corresponding to Ala448Thr amino acid substitution in and exhibit an intermittent drug response to terbinafine. Comparative genomics analysis with available genomes of / species complex revealed a similar genome architecture and identified large number of genes associated with virulence and pathogenicity, namely, lipases, proteases, LysM domain-containing factors, carbon metabolism enzymes and cytochrome P450 enzymes, in all the genomes. An analysis of single amino acid polymorphisms (SAPs) in the protein sequences of subtilisin and lipase enzyme families identified a higher frequency of SAPs in functionally important proteins, Sub3 and Sub6 and their possible use in multilocus phylogenetic analysis of / species complex. The whole genome sequences of clinical isolates provided in this report will, hence, serve as a key reference point for investigation of clinical strains and emerging drug resistance among dermatophytes originating from different parts of the world.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13205-021-02950-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353060PMC
September 2021

Dosimetric impact of Acuros XB on cervix radiotherapy using RapidArc technique: a dosimetric study.

Rep Pract Oncol Radiother 2021 12;26(4):582-589. Epub 2021 Aug 12.

Medical Physics Division and Radiation Oncology Department, Rajiv Gandhi Cancer Institute and Research Center, New Delhi, India.

Background: Acuros XB (AXB) may predict better rectal toxicities and treatment outcomes in cervix carcinoma. The aim of the study was to quantify the potential impact of AXB computations on the cervix radiotherapy using the RapidArc (RA ) technique as compared to anisotropic analytical algorithm (AA) computations.

Materials And Methods: A cohort of 30 patients previously cared for cervix carcinoma (stages II-IIIB) was selected for the present analysis. The RA plans were computed using AA and AXB dose computation engines under identical beam setup and MLC pattern.

Results: There was no significant (p > 0.05) difference in D and D to the planning target volume (PTV); moreover, a significant (p < 0.05) rise was noticed for mean dose to the PTV (0.26%), D (0.26%), D (0.80%) and V (44.24%) for AXB computation as compared to AA computations. Further, AXB estimated a significantly (p < 0.05) lower value for maximum and minimum dose to the PTV. Additionally, there was a significant (p < 0.05) reduction observed in mean dose to organs at risk (OARs) for AXB computation as compared to AA, though the reduction in mean dose was non-significant (p > 0.05) for the rectum. The maximum difference observed was 4.78% for the rectum V, 1.72%, 1.15% in mean dose and 2.22%, 1.48% in D of the left femur and right femur, respectively, between AA and AXB dose estimations.

Conclusion: For similar target coverage, there were significant differences observed between the AAA and AXB computations. AA underestimates the V of the rectum and overestimates the mean dose and D for femoral heads as compared to AXB. Therefore, the use of AXB in the case of cervix carcinoma may predict better rectal toxicities and treatment outcomes in cervix carcinoma using the RA technique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5603/RPOR.a2021.0074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382073PMC
August 2021

Characterization of lipopeptide biosurfactant produced by a carbazole-degrading bacterium Roseomonas cervicalis: The role of biosurfactant in carbazole solubilisation.

J Appl Microbiol 2021 Aug 20. Epub 2021 Aug 20.

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India.

Aim: Characterization of biosurfactant produced by a carbazole-degrading bacterium Roseomonas cervicalis and proteomic analysis of intracellular proteins of bacterium while growing on glucose and carbazole medium.

Methods And Results: The bacterium R. cervicalis was isolated from a soil sample contaminated with crude petroleum oil. PCR amplification ascertained the existence of some hydrocarbon-degrading catabolic genes (alkB and PAH-RHDα, C12O, and C23O) in the bacterial genome. GC-MS and RP-HPLC analyses demonstrated 62% and 60% carbazole degradation, respectively, by R. cervicalis 144 h post-incubation at 37℃ and pH 6.5. Due to the paucity of protein databases, expressions of only 29 and 14 intracellular proteins were explicitly recognized and quantitated by mass spectrometry analysis when R. cervicalis was grown in carbazole and glucose medium, respectively. FTIR, NMR and HR-MS/MS analyses demonstrated the lipopeptide nature of the purified biosurfactant produced by R. cervicalis. The biosurfactant is also presumed to assist in the solubilization of carbazole.

Conclusion: The isolated R. cervicalis strain is a potential candidate for the bioremediation of carbazole in petroleum-oil-contaminated sites.

Significance And Impact Of The Study: This is the first report of the promising R. cervicalis strain proficient in carbazole biodegradation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jam.15258DOI Listing
August 2021

Shabd: A psycholinguistic database for Hindi.

Behav Res Methods 2021 Aug 6. Epub 2021 Aug 6.

Department of Computer Science and Engineering, Indian Institute of Technology Kanpur, Kanpur, India.

We present Shabd, a psycholinguistic database in Hindi. It is based on a corpus of 1.4 billion words from electronic newspapers and news websites. Word frequencies and part of speech information have been derived and are made available in a cleaned list of 34 thousand hand-selected words, and a list of 96 thousand words observed with a frequency of more than 100 times in the corpus. Next to the Shabd database, we also make a list with all 2.3 million word types available and a list with the 2.5 million most frequent word pairs (word bigrams). The quality of the word frequency measure was tested in two lexical decision tasks. We observed that the Shabd word frequencies outperform existing frequencies based on smaller corpora of newspapers but not the Worldlex word frequencies based on an analysis of blogs. We also observed that word frequency accounts for as much variance as contextual diversity (operationalized as the number of documents in which the words were observed). The Shabd database is freely available for research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3758/s13428-021-01625-2DOI Listing
August 2021

Combining SARS-CoV-2 Proofreading Exonuclease and RNA-Dependent RNA Polymerase Inhibitors as a Strategy to Combat COVID-19: A High-Throughput Screening.

Front Microbiol 2021 20;12:647693. Epub 2021 Jul 20.

Amity Institute of Integrative Sciences and Health, Amity University Haryana, Gurgaon, India.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions of people worldwide. Currently, many clinical trials in search of effective COVID-19 drugs are underway. Viral RNA-dependent RNA polymerase (RdRp) remains the target of choice for prophylactic or curative treatment of COVID-19. Nucleoside analogs are the most promising RdRp inhibitors and have shown effectiveness , as well as in clinical settings. One limitation of such RdRp inhibitors is the removal of incorporated nucleoside analogs by SARS-CoV-2 exonuclease (ExoN). Thus, ExoN proofreading activity accomplishes resistance to many of the RdRp inhibitors. We hypothesize that in the absence of highly efficient antivirals to treat COVID-19, combinatorial drug therapy with RdRp and ExoN inhibitors will be a promising strategy to combat the disease. To repurpose drugs for COVID-19 treatment, 10,397 conformers of 2,240 approved drugs were screened against the ExoN domain of nsp14 using AutoDock VINA. The molecular docking approach and detailed study of interactions helped us to identify dexamethasone metasulfobenzoate, conivaptan, hesperidin, and glycyrrhizic acid as potential inhibitors of ExoN activity. The results were further confirmed using molecular dynamics (MD) simulations and molecular mechanics combined with generalized Born model and solvent accessibility method (MM-GBSA) calculations. Furthermore, the binding free energy of conivaptan and hesperidin, estimated using MM-GBSA, was -85.86 ± 0.68 and 119.07 ± 0.69 kcal/mol, respectively. Based on docking, MD simulations and known antiviral activities, and conivaptan and hesperidin were identified as potential SARS-CoV-2 ExoN inhibitors. We recommend further investigation of this combinational therapy using RdRp inhibitors with a repurposed ExoN inhibitor as a potential COVID-19 treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.647693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329495PMC
July 2021

Effects of piperazine and EDTA in garden snail towards electrolytic variation and antimicrobial activities.

Environ Sci Pollut Res Int 2021 Dec 29;28(46):65911-65922. Epub 2021 Jul 29.

Department of Environmental Science, Central University of Himachal Pradesh, Dharamshala, 176215, India.

The present study has been carried out to evaluate the effects of piperazine and EDTA (ethylenediaminetetraacetic acid) in the garden snail, Cornu aspersum. EDTA and piperazine-like chemicals are widely used in various pharmaceutical, household, and industrial applications. The snails after collection were kept in different earthen pots and treated with these chemicals at different concentrations. A higher concentration of these chemicals led to a change in foot color from light to dark brown and loss in average weight with time. It has been found that a 10-fold increase in piperazine and EDTA concentration reduces weight by approximately 12.7- and 11.6-fold, respectively. Further, the study provides an insight into the altered antimicrobial activity of crude extract when treated with ligands. Additionally, the variations in the electrolytes in the mucus sample have been observed with the mean standard deviation (± SD) of 6.4 and 2.4 for Na and K ions, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15543-5DOI Listing
December 2021
-->