Publications by authors named "Pauline Panzuti"

6 Publications

  • Page 1 of 1

Extra-auricular lesions of proliferative and necrotizing otitis externa in three kittens.

Vet Dermatol 2021 May 7. Epub 2021 May 7.

Université de Lyon, VetAgro Sup, UP ICE, Marcy l'Etoile, F-69280, France.

Background: Proliferative and necrotising otitis externa (PNOE) is a rare disorder in cats with poorly understood pathogenesis. Extra-auricular (EA) lesions recently have been mentioned in a textbook and in one case report.

Objectives: To describe EA lesions associated with PNOE in three kittens.

Animals: A 6-month-old female domestic short hair (DSH) cat (Case 1), an 8-month-old female DSH cat (Case 2) and a 5-month-old female DSH cat (Case 3).

Methods And Results: All cases exhibited classical lesions of PNOE associated with EA lesions, generalised (cases 1 and 3) or limited to eyelids (Case 2). Lesions were characterised by thick, adherent, hyperkeratotic papules coalescing to plaques and attempts to remove the hyperkeratotic plaques resulted in erosions. Histopathological examinations revealed classical features of PNOE: severe acanthosis associated with a marked lymphocytic exocytosis, satellitosis and apoptotic keratinocytes at all levels of the epidermis and the outer root sheath of hair follicles. Cases 2 and 3 resolved spontaneously. Case 1 remained stable with topical tacrolimus and oral prednisolone after a short course of ciclosporin.

Conclusions And Clinical Importance: This report describes EA lesions of PNOE in three kittens. Such lesions may be underdiagnosed, and this report emphasises the role of a thorough clinical inspection in PNOE cases.
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http://dx.doi.org/10.1111/vde.12968DOI Listing
May 2021

A moisturizer formulated with glycerol and propylene glycol accelerates the recovery of skin barrier function after experimental disruption in dogs.

Vet Dermatol 2020 Oct 6;31(5):344-e89. Epub 2020 Jul 6.

VetAgro Sup, UP ICE, Université de Lyon, 69280, Marcy l'Étoile, France.

Background: Moisturizers are foundational therapies for human atopic dermatitis. In veterinary medicine, the use of moisturizers has been recommended by an expert committee to alleviate skin dryness that would occur, for example, in canine atopic dermatitis (cAD). However, little is known regarding the effects of moisturizers on the skin barrier.

Hypothesis/objectives: To investigate the effects of a moisturizer on skin barrier recovery in a canine model of chronic mechanical barrier disruption.

Animals: Six healthy beagle dogs maintained in a laboratory setting.

Methods And Materials: A model of chronic skin barrier disruption was simulated by tape stripping on both sides of the thorax. The moisturizer then was applied twice daily for one week to one side of the thorax, while the other hemithorax was left untreated. The effects were evaluated by measurement of transepidermal water loss (TEWL) at various times during skin barrier recovery, and by histological assessment of the disrupted skin one week after moisturizer application.

Results: Overall, TEWL was reduced, epidermal thickness was lower, stratum corneum thickness was greater and the intensity of the dermal inflammatory infiltrate was reduced for treated sites.

Conclusions And Clinical Importance: These results suggest a potential benefit of the moisturizer for improving skin barrier function, which is frequently altered in chronic inflammatory dermatoses such as cAD.
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http://dx.doi.org/10.1111/vde.12859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586792PMC
October 2020

Long-term successful treatment of a donkey with cutaneous lupus erythematous with methotrexate.

Vet Dermatol 2020 Aug 15;31(4):313-e78. Epub 2020 Apr 15.

Université Lumière Lyon 2, VetAgro Sup, UP Interactions Cellules Environnement, 1 Avenue Bourgelat, 69280, Marcy l'Etoile, France.

Cutaneous lupus erythematosus (CLE) is a rare immune-mediated dermatitis. To the best of the authors' knowledge it has not been described in donkeys. A 5-year-old male neutered donkey, living in south-east France, was diagnosed with CLE. Clinical signs included generalized symmetrical areas of alopecia, erythema, crusting and scales. Diagnostic tests included examination of skin biopsy samples by histopathological and immunohistochemical analysis which demonstrated an interface dermatitis with CD8+ T cells. The skin condition was successfully treated initially with glucocorticoids and methotrexate; successful long-term maintenance was associated with administration of methotrexate.
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http://dx.doi.org/10.1111/vde.12847DOI Listing
August 2020

Pharmacokinetics of low-dose methotrexate in horses.

J Vet Pharmacol Ther 2020 Sep 26;43(5):461-469. Epub 2020 Mar 26.

Université de Lyon, VetAgro Sup, UPSP ICE 'Interactions Cellules Environnement', Marcy l'Etoile, France.

This study aimed to investigate both the pharmacokinetic behavior and tolerance of methotrexate (MTX) in horses to design a specific dosing regimen as a new immunomodulatory drug for long-term treatment. To determine the primary plasma pharmacokinetic variables after single intravenous, subcutaneous or oral administration, six horses were administered 0.3 mg/kg MTX in a crossover design study. After a 10-week washout, MTX was administered subcutaneously to three of the six previously treated horses at a dose of 0.3 mg/kg once per week for 3 months. In both studies, MTX and metabolite concentrations were measured using LC-MS/MS. The absolute bioavailability of MTX was 73% following subcutaneous administration but less than 1% following oral administration. The plasma clearance was 1.54 ml min  kg (extraction ratio = 2%). After 24 hr, plasma concentrations were below the LOQ. No adverse effects were noted except for a moderate reversible elevation in liver enzymes (GLDH). With regards to the main metabolites of MTX, very low concentrations of 7-hydroxy-MTX were found, whereas polyglutamated forms (mainly short chains) were found in red blood cells. A subcutaneous dose of 0.2 mg kg  week may be safe and relevant in horses, although this has yet to be clinically confirmed.
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http://dx.doi.org/10.1111/jvp.12857DOI Listing
September 2020

Equine pastern vasculitis in a horse associated with a multidrug-resistant Pseudomonas aeruginosa isolate.

Vet Dermatol 2020 Jun 12;31(3):247-e55. Epub 2020 Jan 12.

UP Interactions Cellules Environnement, VetAgro Sup, Université de Lyon, 1 Avenue Bourgelat, 69280, Marcy l'Etoile, France.

Background: Equine pastern vasculitis is an uncommon disorder in horses. Underlying causes are difficult to assess, especially bacterial infections.

Clinical Summary: A 13-year-old French saddle gelding horse presented for evaluation of a six weeks history of pastern dermatitis. Histopathological examination of skin biopsy samples revealed small vessel vasculitis. A pure growth of a multidrug-resistant Pseudomonas aeruginosa (MRPA) was obtained from a deep skin biopsy. Clinical remission was observed after a six week course of enrofloxacin and lesions did not recur.

Conclusions And Clinical Importance: To the best of the authors' knowledge, this is the first report of a pastern vasculitis associated with MRPA and successfully treated with a six week course of enrofloxacin.
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http://dx.doi.org/10.1111/vde.12830DOI Listing
June 2020

Granulomatous mural folliculitis in a dog treated with ciclosporin and methotrexate.

Vet Dermatol 2020 Apr 6;31(2):170-174. Epub 2020 Jan 6.

VetAgro Sup, UP Interactions Cellules Environnement, Université de Lyon, 1 Avenue Bourgelat, 69280, Marcy l'Etoile, France.

Background: Canine granulomatous mural folliculitis is a very rare cause of scarring alopecia with unknown pathogenesis.

Hypothesis/objectives: To report a case of granulomatous mural folliculitis in a dog treated with ciclosporin (Cs) and methotrexate (MTX).

Animal: A 13-year-old spayed female Pyrenean shepherd dog with a one month history of scarring alopecia.

Methods And Results: Histopathological examination revealed a granulomatous and lymphocytic mural and necrotizing folliculitis and furunculosis. Immunochemistry, using antibodies for CD3, CD204, CD206, IBA-1 and CD208, revealed that CD3 lymphocytes were infiltrating the outer root sheath along with IBA-1 or CD204 cells. Ciclosporin (5 mg/kg once daily) and MTX (0.25 mg/kg once weekly then 0.5 mg/kg once weekly) were initiated simultaneously, and Cs was stopped after stabilization of the lesions. The dog's skin disease was stable for six months.

Conclusions And Clinical Importance: To the best of the authors' knowledge, this is the first report of the long-term management of a granulomatous mural folliculitis in a dog. Ciclosporin and MTX appeared to be an effective treatment option. Additional treated cases are needed to assess the effectiveness of MTX in canine immune-mediated diseases.
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http://dx.doi.org/10.1111/vde.12825DOI Listing
April 2020