Paul J Lehner

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Paul J Lehner

Publications by authors named "Paul J Lehner"

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83Publications

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The HUSH complex cooperates with TRIM28 to repress young retrotransposons and new genes.

Genome Res 2018 06 4;28(6):836-845. Epub 2018 May 4.

Infection and Immunity, University College London, London WC1E 6BT, United Kingdom.

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June 2018

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

EMBO Rep 2018 May 8;19(5). Epub 2018 Mar 8.

Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK

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May 2018

Regulation of Human γδ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters.

Front Immunol 2018 4;9:662. Epub 2018 Apr 4.

Division of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United Kingdom.

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April 2018

Neuropathic MORC2 mutations perturb GHKL ATPase dimerization dynamics and epigenetic silencing by multiple structural mechanisms.

Nat Commun 2018 Feb 13;9(1):651. Epub 2018 Feb 13.

Department of Medicine, MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, University of Cambridge, Cambridge, CB2 0QH, UK.

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February 2018

MARCH9-mediated ubiquitination regulates MHC I export from the TGN.

Immunol Cell Biol 2017 10 31;95(9):753-764. Epub 2017 May 31.

Centre d'Immunologie de Marseille-Luminy (CIML), Aix Marseille Université, CNRS, INSERM, Marseille, France.

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October 2017

A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface.

J Cell Sci 2017 Oct 3;130(19):3234-3247. Epub 2017 Aug 3.

University of Cambridge Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK

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October 2017

Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation.

J Cell Sci 2017 Sep 25;130(17):2883-2892. Epub 2017 Jul 25.

Dept. Medical Microbiology, University Medical Center Utrecht, 3584CX Utrecht, The Netherlands

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September 2017

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.

Nat Genet 2017 Jul 5;49(7):1035-1044. Epub 2017 Jun 5.

Department of Medicine, Cambridge Institute for Medical Research, Cambridge Biomedical Campus, Cambridge, CB2 0XY, UK.

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July 2017

Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1α Stability in Aerobic Conditions.

Cell Metab 2016 Nov 27;24(5):740-752. Epub 2016 Oct 27.

Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK. Electronic address:

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November 2016

ATF7IP-Mediated Stabilization of the Histone Methyltransferase SETDB1 Is Essential for Heterochromatin Formation by the HUSH Complex.

Cell Rep 2016 10;17(3):653-659

Department of Medicine, Cambridge Institute for Medical Research, Cambridge Biomedical Campus, Cambridge CB2 0XY, UK. Electronic address:

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October 2016

NOTCH1 mediates a switch between two distinct secretomes during senescence.

Nat Cell Biol 2016 09 15;18(9):979-92. Epub 2016 Aug 15.

University of Cambridge, Cancer Research UK Cambridge Institute, Robinson Way, Cambridge CB2 0RE, UK.

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September 2016

Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants.

Elife 2016 09 30;5. Epub 2016 Sep 30.

Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom.

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September 2016

A Genetic Screen Identifies a Critical Role for the WDR81-WDR91 Complex in the Trafficking and Degradation of Tetherin.

Traffic 2016 08 25;17(8):940-58. Epub 2016 May 25.

Cambridge Institute for Medical Research, University of Cambridge School of Clinical Medicine, Wellcome Trust/MRC Building Biomedical Campus, Cambridge, CB2 0XY, UK.

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August 2016

Manipulation of immunometabolism by HIV-accessories to the crime?

Curr Opin Virol 2016 08 21;19:65-70. Epub 2016 Jul 21.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0XY, UK. Electronic address:

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August 2016

Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens.

Nat Commun 2016 Jun 10;7:11786. Epub 2016 Jun 10.

Department of Medicine, Cambridge Institute for Medical Research, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0XY, UK.

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June 2016

Position-effect variegation revisited: HUSHing up heterochromatin in human cells.

Bioessays 2016 Apr 8;38(4):333-43. Epub 2016 Feb 8.

Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, UK.

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April 2016

A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I.

Biochem J 2015 Oct 28;471(1):79-88. Epub 2015 Jul 28.

Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge School of Clinical Medicine, Wellcome Trust/MRC Building, Biomedical Campus, Hills Road, Cambridge, CB2 0XY, U.K.

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October 2015

Cell Surface Proteomic Map of HIV Infection Reveals Antagonism of Amino Acid Metabolism by Vpu and Nef.

Cell Host Microbe 2015 Oct 1;18(4):409-23. Epub 2015 Oct 1.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0XY, UK. Electronic address:

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October 2015

A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C.

Nat Commun 2015 Oct 16;6:8670. Epub 2015 Oct 16.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0XY, UK.

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October 2015

TRIM5α requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription.

EMBO J 2015 Aug 22;34(15):2078-95. Epub 2015 Jun 22.

MRC Centre of Medical Molecular Virology, Division of Infection and Immunity, University College London, London, UK

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August 2015

Antibody-free magnetic cell sorting of genetically modified primary human CD4+ T cells by one-step streptavidin affinity purification.

PLoS One 2014 31;9(10):e111437. Epub 2014 Oct 31.

Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, United Kingdom.

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June 2015

Human cytomegalovirus: taking the strain.

Med Microbiol Immunol 2015 Jun 17;204(3):273-84. Epub 2015 Apr 17.

Department of Medical Microbiology, Henry Wellcome Building, Cardiff University School of Medicine, Heath Park, Cardiff, CF14 4XN, UK,

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June 2015

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Science 2015 Jun 28;348(6242):1481-1485. Epub 2015 May 28.

Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK.

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June 2015

UBE2L3 polymorphism amplifies NF-κB activation and promotes plasma cell development, linking linear ubiquitination to multiple autoimmune diseases.

Am J Hum Genet 2015 Feb 29;96(2):221-34. Epub 2015 Jan 29.

Department of Medical and Molecular Genetics, King's College London, London SE1 9RT, UK. Electronic address:

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February 2015

TMEM129 is a Derlin-1 associated ERAD E3 ligase essential for virus-induced degradation of MHC-I.

Proc Natl Acad Sci U S A 2014 Aug 16;111(31):11425-30. Epub 2014 Jul 16.

Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge CB2 0XY, United Kingdom;

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August 2014

Cleavage by signal peptide peptidase is required for the degradation of selected tail-anchored proteins.

J Cell Biol 2014 Jun;205(6):847-62

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, England, UK Department of Medicine, University of Cambridge, Cambridge CB2 0QQ, England, UK

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June 2014

Sterol metabolism regulates neuroserpin polymer degradation in the absence of the unfolded protein response in the dementia FENIB.

Hum Mol Genet 2013 Nov 28;22(22):4616-26. Epub 2013 Jun 28.

Department of Medicine, University of Cambridge, Cambridge Institute for Medical Research (CIMR), Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.

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November 2013

A novel post-transcriptional role for ubiquitin in the differential regulation of MHC class I allotypes.

Mol Immunol 2013 Sep 7;55(2):135-8. Epub 2012 Nov 7.

Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, UK.

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September 2013

MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.

Proc Natl Acad Sci U S A 2013 Aug 8;110(35):14290-5. Epub 2013 Aug 8.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom.

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August 2013

Studying ubiquitination of MHC class I molecules.

Methods Mol Biol 2013 ;960:109-125

Cambridge Institute for Medical Research, Addenbrooke's Hospital, University of Cambridge, Cambridge, UK.

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June 2013

Role for the obesity-related FTO gene in the cellular sensing of amino acids.

Proc Natl Acad Sci U S A 2013 Feb 28;110(7):2557-62. Epub 2013 Jan 28.

University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, and National Institute for Health Research Cambridge Biomedical Research Centre, Addenbrooke's Hospital, Cambridge CB2 0QQ, United Kingdom.

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February 2013

Tapasin-related protein TAPBPR is an additional component of the MHC class I presentation pathway.

Proc Natl Acad Sci U S A 2013 Feb 11;110(9):3465-70. Epub 2013 Feb 11.

Department of Pathology, Cambridge Institute of Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom.

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February 2013

Fluorescence-based phenotypic selection allows forward genetic screens in haploid human cells.

PLoS One 2012 22;7(6):e39651. Epub 2012 Jun 22.

Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, United Kingdom.

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November 2012

The RNA-binding E3 ubiquitin ligase MEX-3C links ubiquitination with MHC-I mRNA degradation.

EMBO J 2012 Aug 3;31(17):3596-606. Epub 2012 Aug 3.

Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.

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August 2012

Proteomic plasma membrane profiling reveals an essential role for gp96 in the cell surface expression of LDLR family members, including the LDL receptor and LRP6.

J Proteome Res 2012 Mar 7;11(3):1475-84. Epub 2012 Feb 7.

Cambridge Institute for Medical Research, University of Cambridge , Hills Road, Cambridge, CB2 0XY, United Kingdom.

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March 2012

Endosomal transport via ubiquitination.

Trends Cell Biol 2011 Nov 28;21(11):647-55. Epub 2011 Sep 28.

Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA.

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November 2011

HRD1 and UBE2J1 target misfolded MHC class I heavy chains for endoplasmic reticulum-associated degradation.

Proc Natl Acad Sci U S A 2011 Feb 18;108(5):2034-9. Epub 2011 Jan 18.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom.

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February 2011

What has the study of the K3 and K5 viral ubiquitin E3 ligases taught us about ubiquitin-mediated receptor regulation?

Viruses 2011 Feb 28;3(2):118-31. Epub 2011 Jan 28.

School of Clinical Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.

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February 2011

First report of Salmonella enterica serotype paratyphi A azithromycin resistance leading to treatment failure.

J Clin Microbiol 2010 Dec 13;48(12):4655-7. Epub 2010 Oct 13.

Department of Infectious Diseases, Addenbrooke’s Hospital, Cambridge, United Kingdom.

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December 2010

RNA-binding E3 ubiquitin ligases: novel players in nucleic acid regulation.

Biochem Soc Trans 2010 Dec;38(6):1621-6

Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.

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December 2010

Identification of a lysosomal pathway regulating degradation of the bone morphogenetic protein receptor type II.

J Biol Chem 2010 Nov 24;285(48):37641-9. Epub 2010 Sep 24.

From theDepartment of Medicine, University of Cambridge School of Clinical Medicine, Box 157, Addenbrooke's Hospital, Hills Road, Cambridge, Cambridgeshire CB2 0QQ, United Kingdom.

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November 2010

Ubiquitination of lysine-331 by Kaposi's sarcoma-associated herpesvirus protein K5 targets HFE for lysosomal degradation.

Proc Natl Acad Sci U S A 2010 Sep 30;107(37):16240-5. Epub 2010 Aug 30.

Department of Pathology, Immunology Division, University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, United Kingdom.

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September 2010

Comparative analysis of techniques to purify plasma membrane proteins.

J Biomol Tech 2010 Sep;21(3):108-15

Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.

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September 2010

Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression.

J Immunol 2010 Jun 12;184(12):6978-85. Epub 2010 May 12.

Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, University of Cambridge, Cambridge, United Kingdom.

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June 2010

Efficient internalization of MHC I requires lysine-11 and lysine-63 mixed linkage polyubiquitin chains.

Traffic 2010 Feb 17;11(2):210-20. Epub 2009 Nov 17.

School of Clinical Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.

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February 2010

Jenner's irony: cowpox taps into T cell evasion.

Cell Host Microbe 2009 Nov;6(5):395-7

Section of Medical Microbiology, Cardiff University, Cardiff CF14 4XX, UK.

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November 2009

The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.

J Cell Biol 2009 Sep 31;186(5):685-92. Epub 2009 Aug 31.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB20XY, England, UK.

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September 2009

Stable isotope labeling by amino acids in cell culture and differential plasma membrane proteome quantitation identify new substrates for the MARCH9 transmembrane E3 ligase.

Mol Cell Proteomics 2009 Aug 20;8(8):1959-71. Epub 2009 May 20.

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom.

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August 2009

The trafficking and regulation of membrane receptors by the RING-CH ubiquitin E3 ligases.

Exp Cell Res 2009 May 5;315(9):1593-600. Epub 2008 Nov 5.

Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB20XY, UK.

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May 2009

Viral avoidance and exploitation of the ubiquitin system.

Nat Cell Biol 2009 May;11(5):527-34

Division of Protein and Nucleic Acid Chemistry, Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.

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May 2009

ESCRT proteins and the regulation of endocytic delivery to lysosomes.

Biochem Soc Trans 2009 Feb;37(Pt 1):178-80

Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.

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February 2009

The ubiquitin E3 ligase MARCH7 is differentially regulated by the deubiquitylating enzymes USP7 and USP9X.

Traffic 2008 Jul 11;9(7):1130-45. Epub 2008 Apr 11.

Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.

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July 2008

Natural killer cell evasion by an E3 ubiquitin ligase from Kaposi's sarcoma-associated herpesvirus.

Biochem Soc Trans 2008 Jun;36(Pt 3):459-63

Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.

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June 2008

Down-regulation of NKG2D and NKp80 ligands by Kaposi's sarcoma-associated herpesvirus K5 protects against NK cell cytotoxicity.

Proc Natl Acad Sci U S A 2008 Feb 29;105(5):1656-61. Epub 2008 Jan 29.

Cambridge Institute for Medical Research and Department of Medicine, University of Cambridge, Hills Road, Cambridge CB2 0XY, United Kingdom.

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February 2008

HIV-1 Nef-induced down-regulation of MHC class I requires AP-1 and clathrin but not PACS-1 and is impeded by AP-2.

Mol Biol Cell 2007 Sep 20;18(9):3351-65. Epub 2007 Jun 20.

University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, United Kingdom.

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September 2007

Structure and function: heat shock proteins and adaptive immunity.

J Immunol 2007 Aug;179(4):2035-40

Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, United Kingdom.

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August 2007

MARCH-IX mediates ubiquitination and downregulation of ICAM-1.

FEBS Lett 2007 Jan 8;581(1):45-51. Epub 2006 Dec 8.

Department of Medicine, Cambridge Institute of Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, UK.

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January 2007

Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5.

Science 2006 Oct;314(5798):454-8

Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, UK.

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October 2006

Lysine-63-linked ubiquitination is required for endolysosomal degradation of class I molecules.

EMBO J 2006 Apr 6;25(8):1635-45. Epub 2006 Apr 6.

Department of Medicine, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, UK.

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April 2006

Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII.

J Biol Chem 2006 Feb 20;281(8):5094-105. Epub 2005 Dec 20.

Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, United Kingdom.

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February 2006

Loss of function of a lupus-associated FcgammaRIIb polymorphism through exclusion from lipid rafts.

Nat Med 2005 Oct 18;11(10):1056-8. Epub 2005 Sep 18.

Cambridge Institute for Medical Research and Department of Medicine, University of Cambridge, Cambridge CB2 2XY, UK.

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October 2005

Downregulation of cell surface receptors by the K3 family of viral and cellular ubiquitin E3 ligases.

Immunol Rev 2005 Oct;207:112-25

Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, UK.

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October 2005

Solution structure of the Kaposi's sarcoma-associated herpesvirus K3 N-terminal domain reveals a Novel E2-binding C4HC3-type RING domain.

J Biol Chem 2004 Dec 30;279(51):53840-7. Epub 2004 Sep 30.

Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, UK.

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December 2004

Viral degradation of the MHC class I peptide loading complex.

Immunity 2004 Mar;20(3):305-17

Division of Virology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom.

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March 2004

Recent developments in MHC-class-I-mediated antigen presentation.

Curr Opin Immunol 2004 Feb;16(1):82-9

Lab 5.19, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Rd, CB2 2XY Cambridge, UK.

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February 2004

HSP70 peptide binding mutants separate antigen delivery from dendritic cell stimulation.

Immunity 2004 Jan;20(1):95-106

Division of Immunology, Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, CB2 2XY, Cambridge, United Kingdom.

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January 2004

Tapasin is a facilitator, not an editor, of class I MHC peptide binding.

J Immunol 2003 Nov;171(10):5287-95

Carter Immunology Center and Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA.

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November 2003

The calculus of immunity: quantitating antigen processing.

Authors:
Paul J Lehner

Immunity 2003 Mar;18(3):315-7

Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, CB2 2XY, Cambridge, United Kingdom.

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March 2003

The ABC-transporter signature motif is required for peptide translocation but not peptide binding by TAP.

Eur J Immunol 2003 Feb;33(2):422-7

Division of Immunology, Department of Pathology, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, GB.

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February 2003

Ubiquitylation of MHC class I by the K3 viral protein signals internalization and TSG101-dependent degradation.

EMBO J 2002 May;21(10):2418-29

Department of Pathology, Division of Immunology, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, UK.

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May 2002