Patrick S Stayton

Patrick S Stayton

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Patrick S Stayton

Patrick S Stayton

Publications by authors named "Patrick S Stayton"

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100Publications

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Core-Cross-Linked Nanoparticles Reduce Neuroinflammation and Improve Outcome in a Mouse Model of Traumatic Brain Injury.

ACS Nano 2017 09 16;11(9):8600-8611. Epub 2017 Aug 16.

Department of BioEngineering, Molecular Engineering and Sciences Institute , Box 355061, Seattle, Washington 98195, United States.

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September 2017

Enzyme-Cleavable Polymeric Micelles for the Intracellular Delivery of Proapoptotic Peptides.

Mol Pharm 2017 05 30;14(5):1450-1459. Epub 2017 Mar 30.

Department of Bioengineering and Molecular Engineering and Sciences Institute, University of Washington , Seattle, Washington 98195, United States.

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May 2017

Orientation and conformation of osteocalcin adsorbed onto calcium phosphate and silica surfaces.

Biointerphases 2017 05 18;12(2):02D411. Epub 2017 May 18.

National ESCA and Surface Analysis Center for Biomedical Problems, University of Washington, Seattle, Washington 98195-1653; Department of Bioengineering, University of Washington, Seattle, Washington 98195-1653; and Department of Chemical Engineering, University of Washington, Seattle, Washington 98195-1653.

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May 2017

A Stimuli-Responsive, Binary Reagent System for Rapid Isolation of Protein Biomarkers.

Anal Chem 2016 11 14;88(21):10404-10410. Epub 2016 Oct 14.

Department of Bioengineering, University of Washington , Seattle, Washington 98195, United States.

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November 2016

Nanostructured glycopolymer augmented liposomes to elucidate carbohydrate-mediated targeting.

Nanomedicine 2016 10 14;12(7):2031-2041. Epub 2016 May 14.

Department of Bioengineering, The University of Washington, Seattle, WA, USA. Electronic address:

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October 2016

A Streptavidin Binding Site Mutation Yields an Unexpected Result: An Ionized Asp128 Residue Is Not Essential for Strong Biotin Binding.

Biochemistry 2016 09 8;55(37):5201-3. Epub 2016 Sep 8.

Center for Structural Biology and Department of Chemistry, Vanderbilt University , Nashville, Tennessee 37235-1822, United States.

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September 2016

Editorial.

J Control Release 2016 06;231

Department of Bioengineering, Molecular Engineering and Sciences Institute, University of Washington, Seattle 98195, USA.

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June 2016

Antibody targeting facilitates effective intratumoral siRNA nanoparticle delivery to HER2-overexpressing cancer cells.

Oncotarget 2016 Feb;7(8):9561-75

Clinical Research Division and Center for Intracellular Delivery of Biologics, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

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February 2016

Polymer nanostructures synthesized by controlled living polymerization for tumor-targeted drug delivery.

J Control Release 2015 Dec 2;219:345-354. Epub 2015 Sep 2.

Department of Bioengineering and Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, USA. Electronic address:

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December 2015

Improving lateral-flow immunoassay (LFIA) diagnostics via biomarker enrichment for mHealth.

Methods Mol Biol 2015 ;1256:71-84

Department of Bioengineering, University of Washington, Seattle, WA, 98195, USA.

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September 2015

Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS.

Biointerphases 2015 Jun 3;11(2):02A304. Epub 2015 Jun 3.

National ESCA and Surface Analysis Center for Biomedical Problems, Seattle, Washington, 98195; Department of Bioengineering, University of Washington, Seattle, Washington 98195; and Department of Chemical Engineering, University of Washington, Seattle, Washington 98185.

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June 2015

Enhancement of MHC-I antigen presentation via architectural control of pH-responsive, endosomolytic polymer nanoparticles.

AAPS J 2015 Mar 12;17(2):358-69. Epub 2014 Dec 12.

Department of Bioengineering, University of Washington, Box 355061, Seattle, Washington, 98195, USA.

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March 2015

Stimuli-responsive reagent system for enabling microfluidic immunoassays with biomarker purification and enrichment.

Bioconjug Chem 2015 Jan 24;26(1):29-38. Epub 2014 Dec 24.

Department of Bioengineering, University of Washington , Seattle, Washington 98195, United States.

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January 2015

Neutral polymer micelle carriers with pH-responsive, endosome-releasing activity modulate antigen trafficking to enhance CD8(+) T cell responses.

J Control Release 2014 Oct 31;191:24-33. Epub 2014 Mar 31.

Department of Bioengineering, University of Washington, Box 355061, Seattle, WA 98195-1721, USA. Electronic address:

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October 2014

TOF-SIMS 3D imaging of native and non-native species within HeLa cells.

Anal Chem 2013 Nov 5;85(22):10869-77. Epub 2013 Nov 5.

National ESCA and Surface Analysis Center for Biomedical Problems, Departments of ‡Chemical Engineering and §Bioengineering, University of Washington , Seattle, Washington 98195-1653, United States.

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November 2013

Functionalized nanoparticles provide early cardioprotection after acute myocardial infarction.

J Control Release 2013 Sep 9;170(2):287-94. Epub 2013 May 9.

Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan.

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September 2013

Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidin.

Acta Crystallogr D Biol Crystallogr 2013 Jun 11;69(Pt 6):968-77. Epub 2013 May 11.

Department of Biological Structure, University of Washington, Box 357420, Seattle, WA 98195-7420, USA.

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June 2013

A photoinduced nanoparticle separation in microchannels via pH-sensitive surface traps.

Langmuir 2013 May 24;29(18):5388-93. Epub 2013 Apr 24.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.

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May 2013

pH-Responsive nanoparticle vaccines for dual-delivery of antigens and immunostimulatory oligonucleotides.

ACS Nano 2013 May 30;7(5):3912-25. Epub 2013 Apr 30.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.

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May 2013

Melittin-grafted HPMA-oligolysine based copolymers for gene delivery.

Biomaterials 2013 Mar 20;34(9):2318-26. Epub 2012 Dec 20.

Department of Bioengineering, Univeristy of Washington, Seattle, WA 98195, USA.

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March 2013

Neutral polymeric micelles for RNA delivery.

Bioconjug Chem 2013 Mar 22;24(3):398-407. Epub 2013 Feb 22.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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March 2013

Intracellular delivery and trafficking dynamics of a lymphoma-targeting antibody-polymer conjugate.

Mol Pharm 2012 Dec 5;9(12):3506-14. Epub 2012 Nov 5.

Department of Bioengineering, University of Washington , Seattle, Washington 98195-5061, United States.

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December 2012

Multifunctional triblock copolymers for intracellular messenger RNA delivery.

Biomaterials 2012 Oct 9;33(28):6868-76. Epub 2012 Jul 9.

Department of Bioengineering, University of Washington, Box 355061, 3720 15th Ave NE, Seattle, WA 98195, United States.

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October 2012

Multiplexed enrichment and detection of malarial biomarkers using a stimuli-responsive iron oxide and gold nanoparticle reagent system.

ACS Nano 2012 Aug 24;6(8):6776-85. Epub 2012 Jul 24.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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August 2012

Application of living free radical polymerization for nucleic acid delivery.

Acc Chem Res 2012 Jul 13;45(7):1089-99. Epub 2012 Jan 13.

Department of Bioengineering, University of Washington, Seattle, 98195, United States.

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July 2012

Diblock copolymers with tunable pH transitions for gene delivery.

Biomaterials 2012 Mar 12;33(7):2301-9. Epub 2011 Dec 12.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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March 2012

Second-contact shell mutation diminishes streptavidin-biotin binding affinity through transmitted effects on equilibrium dynamics.

Biochemistry 2012 Jan 3;51(2):597-607. Epub 2012 Jan 3.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.

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January 2012

Probing the orientation of electrostatically immobilized Protein G B1 by time-of-flight secondary ion spectrometry, sum frequency generation, and near-edge X-ray adsorption fine structure spectroscopy.

Langmuir 2012 Jan 22;28(4):2107-12. Epub 2011 Dec 22.

National ESCA and Surface Analysis Center for Biomedical Problems, Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA.

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January 2012

RAFT-synthesized graft copolymers that enhance pH-dependent membrane destabilization and protein circulation times.

J Control Release 2011 Oct 15;155(2):167-74. Epub 2011 Jun 15.

Center for Intracellular Delivery of Biologics, Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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October 2011

Streptavidin and its biotin complex at atomic resolution.

Acta Crystallogr D Biol Crystallogr 2011 Sep 9;67(Pt 9):813-21. Epub 2011 Aug 9.

Department of Biological Structure, University of Washington, Seattle, USA.

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September 2011

Anti-CD22 antibody targeting of pH-responsive micelles enhances small interfering RNA delivery and gene silencing in lymphoma cells.

Mol Ther 2011 Aug 31;19(8):1529-37. Epub 2011 May 31.

Clinical Research Division and Center for Intracellular Delivery of Biologics, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.

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August 2011

Synthesis of folate-functionalized RAFT polymers for targeted siRNA delivery.

Biomacromolecules 2011 Jul 10;12(7):2708-14. Epub 2011 Jun 10.

University of Rochester, Department of Biomedical Engineering, Center for Musculoskeletal Research, 308 Robert B. Goergen Hall, Rochester, New York 14627, USA.

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July 2011

Probing orientation of immobilized humanized anti-lysozyme variable fragment by time-of-flight secondary-ion mass spectrometry.

J Biomed Mater Res A 2011 Apr 9;97(1):1-7. Epub 2011 Feb 9.

Department of Chemical Engineering, University of Washington, Box 351750, Seattle, Washington 98195-1750.

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April 2011

Delivery of basic fibroblast growth factor with a pH-responsive, injectable hydrogel to improve angiogenesis in infarcted myocardium.

Biomaterials 2011 Mar 24;32(9):2407-16. Epub 2010 Dec 24.

Department of Bioengineering, University of Washington, 3720 15th Ave NE, Box 355061, Seattle, WA 98195, USA.

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March 2011

Efficient Intracellular Delivery of a Pro-Apoptotic Peptide With A pH-Responsive Carrier.

React Funct Polym 2011 Mar;71(3):261-265

Department of Bioengineering, University of Washington, Box 355061, Seattle, WA.

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March 2011

Differential monocyte/macrophage interleukin-1β production due to biomaterial topography requires the β2 integrin signaling pathway.

J Biomed Mater Res A 2011 Jan 5;96(1):162-9. Epub 2010 Nov 5.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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January 2011

ToF-SIMS imaging and depth profiling of HeLa cells treated with bromodeoxyuridine.

Surf Interface Anal 2011 Jan;43(1-2):354-357

National ESCA and Surface Analysis Center for Biomedical Problems, University of Washington, Seattle, WA 98195.

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January 2011

Intracellular delivery of a protein antigen with an endosomal-releasing polymer enhances CD8 T-cell production and prophylactic vaccine efficacy.

Bioconjug Chem 2010 Dec 2;21(12):2205-12. Epub 2010 Nov 2.

Department of Bioengineering and Center for Intracellular Delivery of Biologics, University of Washington, Seattle Washington 98195, USA.

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December 2010

Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers.

Bioconjug Chem 2010 Dec 11;21(12):2197-204. Epub 2010 Nov 11.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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December 2010

Probing the orientation of surface-immobilized protein G B1 using ToF-SIMS, sum frequency generation, and NEXAFS spectroscopy.

Langmuir 2010 Nov;26(21):16434-41

National ESCA and Surface Analysis Center for Biomedical Problems, Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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November 2010

Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro.

J Biomed Mater Res A 2010 Nov;95(2):649-57

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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November 2010

A helical flow, circular microreactor for separating and enriching "smart" polymer-antibody capture reagents.

Lab Chip 2010 Nov 30;10(22):3130-8. Epub 2010 Sep 30.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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November 2010

Synthesis of statistical copolymers containing multiple functional peptides for nucleic Acid delivery.

Biomacromolecules 2010 Nov 5;11(11):3007-13. Epub 2010 Oct 5.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.

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November 2010

Hyaluronic acid hydrogels with controlled degradation properties for oriented bone regeneration.

Biomaterials 2010 Sep 23;31(26):6772-81. Epub 2010 Jun 23.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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September 2010

Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test strips.

Lab Chip 2010 Sep 6;10(17):2279-82. Epub 2010 Jul 6.

University of Washington, Department of Bioengineering, Box 355061, 3720 15th Ave NE, Seattle, WA 98195-5061, USA.

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September 2010

The Role of Basic Amino Acids in the Molecular Recognition of Hydroxyapatite by Statherin using Solid State NMR.

Surf Sci 2010 Aug;604(15-16):L39-L42

Department of Chemistry, University of Washington, Seattle, Washington 98195.

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August 2010

Thermosensitive liposomes modified with poly(N-isopropylacrylamide-co-propylacrylic acid) copolymers for triggered release of doxorubicin.

Biomacromolecules 2010 Aug;11(8):1915-20

Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.

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August 2010

Injectable pH- and temperature-responsive poly(N-isopropylacrylamide-co-propylacrylic acid) copolymers for delivery of angiogenic growth factors.

Biomacromolecules 2010 Jul;11(7):1833-9

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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July 2010

Multi-technique Characterization of Adsorbed Peptide and Protein Orientation: LK3 and Protein G B1.

J Vac Sci Technol B Microelectron Nanometer Struct Process Meas Phenom 2010 Jul;28(4):C5D1

National ESCA and Surface Analysis Center for Biomedical Problems, Department of Chemical, University of Washington, Seattle, WA 98195.

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July 2010

Intracellular delivery of a proapoptotic peptide via conjugation to a RAFT synthesized endosomolytic polymer.

Mol Pharm 2010 Apr;7(2):468-76

Department of Bioengineering, University of Washington, Seattle Washington 98195, USA.

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April 2010

"Smart" diblock copolymers as templates for magnetic-core gold-shell nanoparticle synthesis.

Nano Lett 2010 Jan;10(1):85-91

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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January 2010

A (13)C{(31)P} REDOR NMR investigation of the role of glutamic acid residues in statherin- hydroxyapatite recognition.

Langmuir 2009 Oct;25(20):12136-43

Department of Chemistry, University of Washington, Seattle, Washington98195, USA.

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October 2009

Dynamic bioprocessing and microfluidic transport control with smart magnetic nanoparticles in laminar-flow devices.

Lab Chip 2009 Jul 16;9(14):1997-2002. Epub 2009 Mar 16.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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July 2009

End-functionalized polymers and junction-functionalized diblock copolymers via RAFT chain extension with maleimido monomers.

Bioconjug Chem 2009 Jun;20(6):1122-8

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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June 2009

Retention and biodistribution of microspheres injected into ischemic myocardium.

J Biomed Mater Res A 2009 Mar;88(3):704-10

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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March 2009

Development of a novel endosomolytic diblock copolymer for siRNA delivery.

J Control Release 2009 Feb 17;133(3):221-9. Epub 2008 Oct 17.

Department of Bioengineering, University of Washington, Seattle, WA 98125, USA.

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February 2009

Antigen delivery with poly(propylacrylic acid) conjugation enhances MHC-1 presentation and T-cell activation.

Bioconjug Chem 2009 Feb;20(2):241-8

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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February 2009

In situ characterization of the degradation of PLGA microspheres in hyaluronic acid hydrogels by optical coherence tomography.

IEEE Trans Med Imaging 2009 Jan;28(1):74-81

Department of Bioengineering, University of Washington, Seattle, WA 98195 USA.

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January 2009

Heparin-regulated delivery of osteoprotegerin promotes vascularization of implanted hydrogels.

J Biomater Sci Polym Ed 2008 ;19(8):1021-34

Department of Bioengineering, University of Washington, Seattle, WA, USA.

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October 2008

Encapsulation and stabilization of indocyanine green within poly(styrene-alt-maleic anhydride) block-poly(styrene) micelles for near-infrared imaging.

J Biomed Opt 2008 Jan-Feb;13(1):014025

University of Washington, Department of Bioengineering, Box 355061, William H. Foege Building, Seattle, Washington 98195-5061, USA.

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June 2008

Dual magnetic-/temperature-responsive nanoparticles for microfluidic separations and assays.

Langmuir 2007 Jun 16;23(13):7385-91. Epub 2007 May 16.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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June 2007

Formation of a novel heparin-based hydrogel in the presence of heparin-binding biomolecules.

Biomacromolecules 2007 Jun 19;8(6):1979-86. Epub 2007 May 19.

Research Center for Biomolecular Nanotechnology and Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju 500-712, South Korea.

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June 2007

Synthesis of monodisperse biotinylated p(NIPAAm)-coated iron oxide magnetic nanoparticles and their bioconjugation to streptavidin.

Langmuir 2007 May 24;23(11):6299-304. Epub 2007 Apr 24.

Department of Chemistry and Biochemistry, Laurentian University, Sudbury, ON, P3E 2C6, Canada.

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May 2007

Solid State NMR Studies of Molecular Recognition at Protein-Mineral Interfaces.

Prog Nucl Magn Reson Spectrosc 2007 May;50(2-3):71-85

Department of Chemistry, University of Washington, Box 351700, Seattle WA 98195, USA, , Tel: 1 (206) 543 7760.

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May 2007

Thermodynamic roles of basic amino acids in statherin recognition of hydroxyapatite.

Biochemistry 2007 Apr 29;46(16):4725-33. Epub 2007 Mar 29.

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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April 2007

Internalization of novel non-viral vector TAT-streptavidin into human cells.

BMC Biotechnol 2007 Jan 2;7. Epub 2007 Jan 2.

NanoScience Center; Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.

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January 2007

PEG-cross-linked heparin is an affinity hydrogel for sustained release of vascular endothelial growth factor.

J Biomater Sci Polym Ed 2006 ;17(1-2):187-97

Department of Bioengineering, University of Washington, Seattle 98195, USA.

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October 2006

Folding of the C-terminal bacterial binding domain in statherin upon adsorption onto hydroxyapatite crystals.

Proc Natl Acad Sci U S A 2006 Oct 23;103(44):16083-8. Epub 2006 Oct 23.

Department of Chemistry, University of Washington, Seattle, WA 98195, USA.

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October 2006

pH-responsive poly(styrene-alt-maleic anhydride) alkylamide copolymers for intracellular drug delivery.

Biomacromolecules 2006 Aug;7(8):2407-14

University of Washington, Department of Bioengineering, Seattle, Washington 98195, USA.

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August 2006

Switchable surface traps for injectable bead-based chromatography in PDMS microfluidic channels.

Lab Chip 2006 Jul 5;6(7):843-8. Epub 2006 May 5.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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July 2006

Thermodynamics of statherin adsorption onto hydroxyapatite.

Biochemistry 2006 May;45(17):5576-86

Department of Bioengineering, University of Washington, Seattle, Washington 98195-1721, USA.

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May 2006

Homonuclear and heteronuclear NMR studies of a statherin fragment bound to hydroxyapatite crystals.

J Phys Chem B 2006 May;110(18):9324-32

Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA.

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May 2006

Poly(N-isopropylacrylamide-co-propylacrylic acid) copolymers that respond sharply to temperature and pH.

Biomacromolecules 2006 May;7(5):1381-5

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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May 2006

Cooperative hydrogen bond interactions in the streptavidin-biotin system.

Protein Sci 2006 Mar 1;15(3):459-67. Epub 2006 Feb 1.

Box 351721, Department of Bioengineering, University of Washington, Seattle, WA 98195-1721, USA.

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March 2006

Semi-interpenetrating network of poly(ethylene glycol) and poly(D,L-lactide) for the controlled delivery of protein drugs.

J Biomater Sci Polym Ed 2005 ;16(2):189-201

University of Washington, Department of Bioengineering, Box 352255, Seattle, WA 98195, USA.

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January 2006

Monocyte activation on polyelectrolyte multilayers.

J Biomater Sci Polym Ed 2005 ;16(2):237-51

Department of Bioengineering, University of Washington, Box 351721, Seattle, WA 98195-1721, USA.

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January 2006

Dual-affinity avidin molecules.

Proteins 2005 Nov;61(3):597-607

NanoScience Center, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.

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November 2005

A REDOR NMR study of a phosphorylated statherin fragment bound to hydroxyapatite crystals.

J Am Chem Soc 2005 Jul;127(26):9350-1

Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.

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July 2005

Design and development of polymers for gene delivery.

Nat Rev Drug Discov 2005 Jul;4(7):581-93

Department of Chemical and Biomolecular Engineering, University of Illinois, Box C-3, 600 South Mathews Avenue, Urbana, IL 61801, USA.

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July 2005

Rational design of composition and activity correlations for pH-responsive and glutathione-reactive polymer therapeutics.

J Control Release 2005 May;104(2):417-27

University of Washington, Department of Bioengineering, Box: 352255, Seattle, WA 98195-2255, USA.

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May 2005

A TAT-streptavidin fusion protein directs uptake of biotinylated cargo into mammalian cells.

Protein Eng Des Sel 2005 Mar 8;18(3):147-52. Epub 2005 Apr 8.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

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March 2005