Publications by authors named "Patrícia V Silva"

12 Publications

  • Page 1 of 1

Percutaneous management of a ruptured sinus of Valsalva aneurysm in an infant.

Cardiol Young 2021 Mar 2:1-3. Epub 2021 Mar 2.

Department of Paediatric Cardiology, Referral Centre for Congenital Cardiac Defects, CHUC, Coimbra, Portugal.

Ruptured sinus of Valsalva aneurysm is a rare entity in children. To our knowledge, this is the youngest child reported in the literature with a sinus of Valsalva ruptured aneurysm being successfully treated percutaneously using an AmplatzerTM Duct Occluder II Additional Sizes device.
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http://dx.doi.org/10.1017/S1047951121000652DOI Listing
March 2021

A case of double-outlet left ventricle, malposition of the great arteries, and superior-inferior ventricular arrangement.

Cardiol Young 2020 Aug 27;30(8):1173-1174. Epub 2020 Jul 27.

Department of Paediatric Cardiology, Referral Centre for Congenital Cardiac Defects, CHUC, Coimbra, Portugal.

Superior-inferior ventricular arrangement with double-outlet left ventricle and malposition of the great arteries is an extremely rare congenital cardiac anomaly. The authors present the case of an infant who presented with cyanosis and respiratory distress. To the best of our knowledge, this is the first case reported.
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http://dx.doi.org/10.1017/S104795112000222XDOI Listing
August 2020

Impact of timing of the anorexigen sibutramine administration on reproductive end-points of male rats.

Basic Clin Pharmacol Toxicol 2020 Dec 21;127(6):525-532. Epub 2020 Jul 21.

Department of Structural and Functional Biology, Institute of Biosciences, UNESP - São Paulo State University, Botucatu, Brazil.

Sibutramine is a non-selective serotonin-norepinephrine reuptake inhibitor orally administered for weight loss. In a previous study, we showed pharmacological mechanisms involved in the reduction of sperm quality and fertility of rats exposed for 30 days to this anorexigen in the light phase of the light-dark (l/d) cycle. It is already known that rodents are nightlife animals, with higher metabolic activity during the dark phase than in the light phase of the light-dark (l/d) cycle. Thus, the present study aimed to investigate whether the deleterious effects on reproductive parameters after sibutramine administration would be enhanced after a shorter period of exposure during the dark phase of the l/d cycle. For this, adult male Wistar rats were treated with sibutramine (10 mg/kg/d) or vehicle for 15 days during the dark phase of the l/d cycle. Sibutramine treatment decreased final body and reproductive organ weights, as well as serum testosterone levels. Sperm transit time through the epididymis was accelerated, and sperm concentration and motility were diminished in the sibutramine-exposed rats. The decrease in sperm concentration was also verified in the epididymal histological sections. In conclusion, the deleterious effects of sibutramine on reproductive parameters of male rats were enhanced when the exposure occurred in the dark phase of the l/d cycle, even after a short exposure duration. Our results reinforce the impact of timing on drug therapeutic action.
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http://dx.doi.org/10.1111/bcpt.13467DOI Listing
December 2020

Effects of the organic UV-filter, 3-(4-methylbenzylidene) camphor, on benthic invertebrates and ecosystem function in artificial streams.

Environ Pollut 2020 May 13;260:113981. Epub 2020 Jan 13.

Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal.

In the last decades, the use of organic ultraviolet-filters (UV-filters) has increased worldwide, and these compounds are now considered emerging contaminants of many freshwater ecosystems. The present study aimed to assess the effects of 3-(4-methylbenzylidene) camphor (4-MBC) on a freshwater invertebrate community and on associated ecological functions. For that, artificial streams were used, and a natural invertebrate benthic community was exposed to sediments contaminated with two concentrations of 4-MBC. Effects were evaluated regarding macroinvertebrate abundance and community structure, as well as leaf decomposition and primary production. Results showed that the macroinvertebrate community parameters and leaf decomposition rates were not affected by 4-MBC exposure. On the other hand, primary production was strongly reduced. This study highlights the importance of higher tier ecotoxicity experiments for the assessment of the effects of low concentrations of organic UV-filters on freshwater invertebrate community structure and ecosystem functioning.
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http://dx.doi.org/10.1016/j.envpol.2020.113981DOI Listing
May 2020

Joint effects of chlorpyrifos and mancozeb on the terrestrial isopod Porcellionides pruinosus: A multiple biomarker approach.

Environ Toxicol Chem 2018 05 6;37(5):1446-1457. Epub 2018 Apr 6.

Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal.

The exposure to pesticides by nontarget soil biota has long been regarded as a serious downside of modern agricultural regimes and the subject of heated debate. Of utmost relevance is the exposure to pesticide mixtures because their effects have been shown to not necessarily reflect the individual toxicity of their components, and even the simple addition of effects may lead to consequences not clearly anticipated. In the present study, a multiple biomarker approach was employed to identify the mechanistic and time effects underlying several single and mixture treatments of chlorpyrifos (CPF) and mancozeb (MCZ) in juveniles and adults of the terrestrial isopod Porcellionides pruinosus. The effects of the individual pesticides and the mixtures at recommended doses were mostly transitory under these controlled conditions and one-pulse exposure. Whereas imbalances were identified on detoxification and oxidative stress-related enzymes, the isopods generally showed the ability to recover through the end of the experiment. However, juveniles displayed greater vulnerability than adults. Most of the differences between life stages occurred in energy-related parameters where distinct performances and stress-handling behaviors were observed, suggesting higher metabolic costs to juveniles. Our results stress that understanding the time dependence of the underlying mechanisms governing the joint effects of the pesticides can help in assessing and anticipating the effects of the pesticide mixtures. Moreover, we emphasize the importance of taking life stage-related differences into consideration when evaluating the environmental risks of pesticides and pesticide mixtures. Environ Toxicol Chem 2018;37:1446-1457. © 2018 SETAC.
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http://dx.doi.org/10.1002/etc.4089DOI Listing
May 2018

Reproductive disorders in female rats after prenatal exposure to betamethasone.

J Appl Toxicol 2017 09 21;37(9):1065-1072. Epub 2017 Mar 21.

Departments of Morphology, Institute of Biosciences, Univ Estadual Paulista-UNESP, Distrito de Rubião Junior s/n°, 18618-970, Botucatu, SP, Brazil.

Betamethasone is the drug of choice for antenatal treatment, promoting fetal lung maturation and decreasing mortality. Previous studies in rats reported male programming and alteration in sperm parameters and sexual behavior following intrauterine betamethasone exposure. The impact on the female reproductive development is not known. In this study, rat female offspring was assessed for sexual development, morphophysiology of the reproductive tract and fertility after maternal exposure to 0.1 mg kg of betamethasone or vehicle on gestational days 12, 13, 18 and 19. The treatment promoted reduction of litter weight on postnatal day 1, morphological masculinization in females, delay in the age of puberty onset, reduction in estrus number, increase in estrous cycle length and increase in luteinizing hormone serum levels and uterus weight. The females from the betamethasone group showed an increase of myometrial uterine area and decrease in endometrial uterine area. These animals also performed less lordosis during the sexual behavior test and showed impaired reproductive performance. The uterus showed higher contraction in the treated group as shown by a pharmacological assay. In conclusion, prenatal betamethasone exposure in rats promoted female masculinization, altered sexual development and reproductive parameters. Copyright © 2017 John Wiley & Sons, Ltd.
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http://dx.doi.org/10.1002/jat.3457DOI Listing
September 2017

Alterations in male rats following in utero exposure to betamethasone suggests changes in reproductive programming.

Reprod Toxicol 2016 08 28;63:125-34. Epub 2016 May 28.

Department of Morphology, Institute of Biosciences, Univ Estadual Paulista-UNESP, Distrito de Rubião Junior s/n°, 18618-970 Botucatu, SP, Brazil.

Antenatal betamethasone is used for accelerating fetal lung maturation for women at risk of preterm birth. Altered sperm parameters were reported in adult rats after intrauterine exposure to betamethasone. In this study, male rat offspring were assessed for reproductive development after dam exposure to betamethasone (0.1mg/kg) or vehicle on Days 12, 13, 18 and 19 of pregnancy. The treatment resulted in reduction in the offspring body weight, delay in preputial separation, decreased seminal vesicle weight, testosterone levels and fertility, and increased testicular weight. In the testis, morphologically abnormal seminiferous tubules were observed, characterized by an irregular cell distribution with Sertoli cell that were displaced towards the tubular lumen. These cells expressed both Connexin 43 (Cx43) and Proliferative Nuclear Cell Antigen (PCNA). In conclusion, intrauterine betamethasone treatment appears to promote reproductive programming and impairment of rat sexual development and fertility due to, at least in part, unusual testicular disorders.
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http://dx.doi.org/10.1016/j.reprotox.2016.05.021DOI Listing
August 2016

Perinatal exposure to insecticide fipronil: effects on the reproductive system in male rats.

Reprod Fertil Dev 2017 Jun;29(6):1130-1143

Department of Morphology, Institute of Biosciences of Botucatu, São Paulo State University, (UNESP) - Distrito de Rubião Junior s/n, 510 - Botucatu, São Paulo State, Brazil.

Fipronil is an insecticide widely used in agriculture, veterinary medicine and public health that has recently been listed as a potential endocrine disrupter. In the present study we evaluated the effects of perinatal exposure to fipronil during the period of sexual brain differentiation and its later repercussions on reproductive parameters in male rats. Pregnant rats were exposed (via gavage) to fipronil (0.03, 0.3 or 3mgkg) from Gestational Day 15 until Postnatal Day 7. Fipronil exposure did not compromise the onset of puberty. In adulthood, there was no effect on organ weight or sperm production. Furthermore, there were no adverse effects on the number of Sertoli cells per seminiferous tubule, testicular and epididymal histomorphometry or histopathology or expression patterns of androgen receptor in the testis. Similarly, no changes were observed in the sexual behaviour or hormone levels. However, in rats exposed to fipronil, changes in sperm motility were observed, with a decrease in motile spermatozoa and an increase in non-mobile spermatozoa, which can compromise sperm quality in these rats. Perinatal exposure to fipronil has long-term effects on sperm parameters, and the epididymis can be a target organ. Additional studies should be undertaken to identify the mechanisms by which fipronil affects sperm motility.
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http://dx.doi.org/10.1071/RD15517DOI Listing
June 2017

Long-term adverse effects on reproductive function in male rats exposed prenatally to the glucocorticoid betamethasone.

Toxicology 2017 Feb 27;376:15-22. Epub 2016 Apr 27.

Departments of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Distrito de Rubião Junior s/n°, 18618-689 Botucatu, SP, Brazil.

Betamethasone is the drug of choice for antenatal treatment, promoting fetal lung maturation, decreasing the incidence of respiratory distress syndrome and neonatal mortality. Previous studies reported that prenatal treatment with this drug reduced testosterone levels, sperm quality and fertility in adult rats. We aimed to further evaluate the reproductive consequences of prenatal betamethasone exposure in male rats. Pregnant Wistar rats (n=13/group) were separated into two groups: control (vehicle) and betamethasone- treated (0.1mg/kg IM) and rats were injected on gestational days 12, 13, 18 and 19. Body weight, sexual behavior, reproductive organ weights, serum hormone levels, accessory glands contractility, sperm parameters, and fertility after in utero artificial insemination were evaluated. Our results showed that prenatal betamethasone exposure provoked a significant reduction in body weight at PND 01 and, at adulthood, decrease in FSH levels, sperm motility and production. Furthermore, seminal vesicle weight was decreased while testicular and ventral prostate weights were increased. Serum LH levels and the percentage of abnormal sperm were significantly increased. Although sexual behavior was not altered, a significant reduction in fertility in the adult rats exposed prenatally to betamethasone was noted. We concluded that prenatal betamethasone exposure leads to long-term reproductive impairment in male rats. These results may have important implications for humans, considering the use of this glucocorticoid in pregnant women.
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http://dx.doi.org/10.1016/j.tox.2016.04.005DOI Listing
February 2017

Toxicity of tributyltin (TBT) to terrestrial organisms and its species sensitivity distribution.

Sci Total Environ 2014 Jan 28;466-467:1037-46. Epub 2013 Aug 28.

Department of Biology & CESAM-Centre for Environmental and Marine Studies, University of Aveiro, 3810-193 Aveiro, Portugal.

The contamination of the terrestrial environment by disposal of tributyltin (TBT) by contaminated harbour sediments, sewage sludge and/or biocide products has been raising concerns and it may pose a risk to soil invertebrates and plants. This study aimed to improve the amount and quality of data for TBT toxicity in soils in order to assess the ecological risk of TBT to the terrestrial ecosystems. For this, bioassays were performed with the species Porcellionides pruinosus, Folsomia candida, Brassica rapa and Triticum aestivum to evaluate the toxic effects of TBT (as chloride) on these species. Additionally, this study contributed to increase the amount of data concerning TBT toxicity on soil dwelling organisms. The results showed a dose-response relationship between TBT concentration and the increase of toxicity in all species tested. These results were collated with results from literature to construct species sensitivity distributions (SSDs) and to calculate the hazardous concentration at 5% (HC₅) for all data, for each type of soil and TBT formulation used. The HC₅ value for TBT in soil was 2.06 mg TBT/kg soil dw. Little information is available concerning the concentrations of TBT in soils. In addition the predicted no-effect concentration (PNEC) value was determined to be 30 μg/kg soil. Only one study was found referring to TBT contaminated soils, and where TBT concentrations were lower than 0.024 μg TBT/kg for the wetland soil. Therefore it can be concluded that the real TBT concentrations determined represent low risk for environmental effects. In conclusion, the construction of SSDs and the calculation of HC5 using all the data available showed to be a more suitable method rather than the construction of several SSDs for each soil and TBT types. Further investigations concerning TBT concentrations and toxicity on soil organisms need to be performed to increase data and improve risk calculations.
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http://dx.doi.org/10.1016/j.scitotenv.2013.08.002DOI Listing
January 2014

Desmoid tumor in patients with familial adenomatous polyposis.

Arq Gastroenterol 2010 Oct-Dec;47(4):373-8

UNICAMP, Brazil.

Context: Desmoid tumors constitute one of the most important extraintestinal manifestations of familial adenomatous polyposis. The development of desmoids is responsible for increasing morbidity and mortality rates in cases of familial adenomatous polyposis.

Objectives: To evaluate the occurrence of desmoid tumors in familial adenomatous polyposis cases following prophylactic colectomy and to present patient outcome.

Methods: Between 1984 and 2008, 68 patients underwent colectomy for familial adenomatous polyposis at the School of Medical Sciences Teaching Hospital, University of Campinas, SP, Brazil. Desmoid tumors were found in nine (13.2%) of these patients, who were studied retrospectively by consulting their medical charts with respect to clinical and surgical data.

Results: Of nine patients, seven (77.8%) were submitted to laparotomy for tumor resection. Median age at the time of surgery was 33.9 years (range 22-51 years). Desmoid tumors were found in the abdominal wall in 3/9 cases (33.3%) and in an intra-abdominal site in the remaining six cases (66.7%). Median time elapsed between ileal pouch-anal anastomosis and diagnosis of desmoid tumor was 37.5 months (range 14-60 months), while the median time between colectomy with ileorectal anastomosis and diagnosis was 63.7 months (range 25-116 months). In 6/9 (66.7%) patients with desmoid tumors, the disease was either under control or there was no evidence of tumor recurrence at a follow-up visit made a mean of 63.1 months later (range 12-240 months).

Conclusions: Desmoid tumors were found in 13.2% of cases of familial adenomatous polyposis following colectomy; therefore, familial adenomatous polyposis patients should be followed-up and surveillance should include abdominal examination to detect signs and symptoms. Treatment options include surgery and clinical management with antiestrogens, antiinflammatory drugs or chemotherapy.
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http://dx.doi.org/10.1590/s0004-28032010000400010DOI Listing
August 2011

Immunoblotting for the serodiagnosis of Helicobacter pylori infection in Brazilian patients with and without gastric carcinoma.

Mem Inst Oswaldo Cruz 2004 Mar 24;99(2):189-93. Epub 2004 Jun 24.

Laboratório de Bacteriologia, Faculdade de Medicina, Universidade Federal de Minas Gerais. Av. Alfredo Balena, 190/4026, 30130-100 Belo Horizonte, MG, Brasil.

We evaluated the performance of a commercial immunoblotting in the serodiagnosis of Helicobacter pylori infection in Brazilian patients. The presence of anti-H. pylori antibodies was also investigated in a group of 20 duodenal ulcer patients after successful treatment. One hundred and ninety one patients were studied. Among the 164 infected patients, 46 had gastric carcinoma. The duodenal ulcer patients were treated with antimicrobial drugs and the eradication of the microorganism was confirmed in all of them one month after the end of the treatment by the 13C-urea breath test. Sera were assayed for H. pylori antibodies using the Helicoblot 2.0 (Genelabs Diagnostics, Singapore). The sensitivity, specificity, positive, and negative predictive values of the test were 93.9%, 92.6%, 98.7%, and 71.4%, respectively. The sensitivity of the test was similar in patients with (93.5%) and without (95.7%) gastric carcinoma. Twenty-four months after the end of the treatment, the band of 116 kDa was still detected in one of the patients. In conclusion, the Helicoblot 2.0 is an accurate test to diagnose H. pylori infection and although it can not be employed to monitor the bacterium eradication, it may be useful for diagnosing past infection, especially in gastric carcinoma patients.
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http://dx.doi.org/10.1590/s0074-02762004000200013DOI Listing
March 2004