Dr. Anandrao Ashok Patil , PhD - North Carolina State University - Postdoctoral Research Scholar

Dr. Anandrao Ashok Patil

PhD

North Carolina State University

Postdoctoral Research Scholar

RALEIGH, NORTH CAROLINA | United States

Main Specialties: Biology

Additional Specialties: Insect Molecular Biology and Entomology

ORCID logohttps://orcid.org/0000-0002-1767-7304

Dr. Anandrao Ashok Patil , PhD - North Carolina State University - Postdoctoral Research Scholar

Dr. Anandrao Ashok Patil

PhD

Introduction

Primary Affiliation: North Carolina State University - RALEIGH, NORTH CAROLINA , United States

Specialties:

Additional Specialties:

Research Interests:

Education

Oct 2014 - Sep 2017
Kyushu University
Ph.D.
Department of Bioresource Sciences, Graduate School of Bioresource and Bioenvironmental Science, Faculty of Agriculture
Sep 2017
Kyushu University, Fukuoka, Japan
Ph.D.
Nov 2012
Tamil Nadu Agricultural University, Coimbatore, India
Master (M.Sc)
Sep 2010 - Oct 2012
Tamil Nadu Agricultural University
Master of Sceince (Biotechnology)
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology
Jul 2010
Marathwada Agricultural University, Parbhani, Maharashtra, India
Bachelor (B.Sc)

Experience

Jan 2019
North Carolina State University
Postdoctoral Researcher
Insect Transgenesis
May 2017 - Apr 2018
Incheon National University
Postdoctoral Researcher
Bioengineering
Aug 2013 - Sep 2014
Seoul National University
Researcher
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences

Publications

5Publications

239Reads

111Profile Views

8PubMed Central Citations

Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived BmN4 cells.

Biochem Biophys Res Commun 2017 11 20;493(2):971-978. Epub 2017 Sep 20.

Laboratory of Insect Genome Science, Department of Bioresource Science, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2017.09.107DOI Listing
November 2017
154 Reads
2.297 Impact Factor

Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell.

Biochem Biophys Res Commun 2017 08 5;490(2):134-140. Epub 2017 Jun 5.

Laboratory of Insect Genome Science, Kyushu University, Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S0006291X173111
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http://dx.doi.org/10.1016/j.bbrc.2017.06.008DOI Listing
August 2017
60 Reads
1 Citation
2.300 Impact Factor

Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus

Journal of Asia-Pacific Entomology

G protein-coupled receptors (GPCRs) are seven transmembrane proteins, which play an essential role in trans- mitting various extracellular signals into cells. For functional and structural analysis of GPCRs, it is necessary to produce active GPCRs in high quantities with outstanding purity. Fortunately, earlier baculovirus expression vec- tor system has been reported as a proven functional GPCR mass-production tool. Therefore, in this study, we se- lected Bombyx mori allatostatin-C neuropeptide receptor BNGR-A1 as a GPCR reporter protein, which has been already proved successful binding of ligand. We confirmed its expression profile in various silkworm tissues and cell lines and then verified its plasma membrane subcellular localization in cultured silkworm BmN4 cells. In addition, we constructed recombinant baculoviruses for BNGR-A1, its ligand allatostatin-C (BmAST-C) and re- lated eight G proteins (Gs, G12, Gα4, Gq, Gβ2, Gβ3, Gβ5 and Gγ) and subsequently monitored the extracellular or intracellular expression of BNGR-A1 by co-infection with its ligand and cognate G proteins-expressing viruses. It is interesting to observe that different combinations of G proteins could result in changes or even undetectable of final yields of BNGR-A1, suggesting the essential roles of G proteins involved in the GPCR expression or stabiliza- tion. The present study demonstrated that co-infection of recombinant viruses expressing Gα4β3γ trimer en- hanced the production of BNGR-A1 on BV fractions. To our knowledge, this is a fine strategy for identifying the specific G protein partner responsible for certain GPCR of interest. These studies would provide a novel idea for improving GPCR expression in the silkworm by baculovirus expression vector system.

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September 2016
7 Reads

Screening of target genes for RNAi in Tetranychus urticae and RNAi toxicity enhancement by chimeric genes.

Pestic Biochem Physiol 2016 Jun 11;130:1-7. Epub 2015 Nov 11.

Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address:

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http://dx.doi.org/10.1016/j.pestbp.2015.11.005DOI Listing
June 2016
15 Reads
3 Citations
2.014 Impact Factor

Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori.

Insect Biochem Mol Biol 2015 Mar 23;58:55-65. Epub 2015 Jan 23.

Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Fukuoka, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.ibmb.2015.01.008DOI Listing
March 2015
10 Reads
4 Citations
3.450 Impact Factor