Publications by authors named "Parveen Bansal"

30 Publications

  • Page 1 of 1

Clonazepam tops the list of potentially inappropriate psychotropic (PIP) medications in older adults with psychiatric illness: A cross-sectional study based on Beers criteria 2019 vs STOPP criteria 2015.

Asian J Psychiatr 2021 Apr 13;58:102570. Epub 2021 Feb 13.

Indian Council of Medical Research Scientist-l, Multidisciplinary Research Unit (Department of Health Research, Government of India), Guru Gobind Singh Medical College, Faridkot, Punjab, 151203, India. Electronic address:

Background: In older adults, polypharmacy and potentially inappropriate psychotropic (PIP) medication use are prominent prescription challenges. However, there is limited information available on the use of PIP medication in older adults having psychiatry illness.

Objective: To find out the most commonly prescribed PIP in tertiary care hospitals of developing countries with respect to Beers criteria 2019 and Screening Tool of Older Persons' Potentially Inappropriate Prescriptions (STOPP) and predictors of PIP.

Methods: A cross-sectional analysis of 456 patients of either sex with a median age of 65 years visiting the outpatient department of psychiatry was performed at the tertiary care hospital of North India with respect to Beers criteria 2019 and STOPP criteria 2015. Bivariate logistic regression was used to figure out the predictors of PIP medication.

Results: Results of the study reflects a staggering number of older adults, (more than 91 % and 73 %) out of total 456 patients were prescribed with at least one PIP medication as per Beers criteria and STOPP criteria, respectively. Long-acting benzodiazepine like clonazepam, chlordiazepoxide were identified as one of the most commonly prescribed PIP medications with respect to the both set of criteria. Further analysis revealed that polypharmacy (≥5 medications with odds Ratio (OR) 17.33, 95% Confidence Interval (CI) 1.42-210.66, P-0.025) as the sole important predictor for PIP medication.

Conclusion: According to the Beers criterion and the STOPP criteria, the use of PIP medicine is very prevalent among older adults with psychiatric illness. The Beers criteria dramatically diagnose more PIP medication than STOPP criteria.
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http://dx.doi.org/10.1016/j.ajp.2021.102570DOI Listing
April 2021

Erdafitinib as a Novel and Advanced Treatment Strategy of Metastatic Urothelial Carcinoma.

Anticancer Agents Med Chem 2021 Jan 20. Epub 2021 Jan 20.

Multidisciplinary Research Unit, Veer Chandra Singh Garhwali Government Institute of Medical Science and Research, Srinagar, Pauri Garhwal. India.

Urothelial carcinoma has become the ninth most common malignancy in the world. Since 1980s, diverse studies and treatment methods came out with their possible effects along with certain limitations. Initially, platinum chemotherapy was considered as first line treatment of the disease. Although it was proved to be effective in the beginning yet most number of cases reported the reoccurrence of the disease. Furthermore, aberrant ligand-dependent and constitutive ligandindependent fibroblast growth factor receptor (FGFR) signalling has been reported in large number of solid tumours including urothelial carcinoma that became the basis for FGFR inhibition for the treatment of the disease. Erdafitinib is a pan-FGFR inhibitor that was recently approved in the USA for the treatment of locally advanced or metastatic FGFR3 or FGFR2 urothelial carcinoma. The drug is also being investigated as a treatment for other cancers including cholangiocarcinoma, liver cancer, non-small cell lung cancer, prostate cancer, lymphoma cancer and oesophageal cancer. This article summarizes the various treatments evolved for bladder cancer till now, brief description of biology of FGFR inhibition, clinical pharmacology and various clinical trials of erdafitinib.
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http://dx.doi.org/10.2174/1871520621666210121093852DOI Listing
January 2021

Prevalence of potentially inappropriate medication and its correlates in elderly hospitalized patients: A cross-sectional study based on Beers criteria.

J Family Community Med 2020 Sep-Dec;27(3):200-207. Epub 2020 Sep 25.

Multi- Disciplinary Research Unit, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India.

Background: The increase in the prescription of potentially inappropriate medication (PIM) in older adults with significant health consequences is a global concern. This study aimed to determine the prevalence of PIM prescription in older adults as identified by Beers criteria 2015 and 2019.

Materials And Methods: A cross-sectional study was carried out in older adults aged >65 years at a tertiary care postgraduate teaching hospital. All patients aged ≥65 years irrespective of their gender, admitted in the medical ward of the hospital with single/multiple comorbidities, and prescribed at least one daily medication, were included in the study. Data of patient history, patient case sheet, medication charts, laboratory reports, as well as radiological examinations test reports were retrieved from their files and were captured in a prevalidated data collection form. SPSS used for data analysis; multivariate logistic regression was used to determine the predictors of PIM prescribing and odds ratios (ORs) and 95% confidence intervals for ORs were computed.

Results: Study included 323 patients; 61.3% were male, 74% patients were 65-70 years of age, and 78% patients were illiterate. The overall prevalence of PIM uses according to the Beers criteria 2015 and 2019 was 60.1% and 61.9%, respectively. No association found between PIMs prescribes and diagnosis category. Male gender, age 76-80 years, and education 10-12th class were found to be significantly related to PIM prescription.

Conclusion: This study reflects a critical view of noncompliance of Beers criteria for geriatric healthcare even in tertiary care hospitals in India. Creatinine clearance rate should be kept in view when prescribing medicines for elderly inpatients.
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http://dx.doi.org/10.4103/jfcm.JFCM_175_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745783PMC
September 2020

Pitfalls in AGS Beers Criteria® Reflect Communication Gap Between Top Healthcare Organizations: Beware or Be Aware.

J Am Geriatr Soc 2020 12 30;68(12):2969-2971. Epub 2020 Sep 30.

Multidisciplinary Research Unit, Department of Health Research, Ministry of Family and Welfare, Government of India, Guru Gobind Singh Medical College and Hospital, Faridkot, India.

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http://dx.doi.org/10.1111/jgs.16859DOI Listing
December 2020

formulations containing benzoic and ascorbic acids as additives: benzene formation during storage and impact of additives on quality parameters.

J Complement Integr Med 2020 Aug 3;18(1):59-65. Epub 2020 Aug 3.

University Center of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, India.

Objectives: formulations are becoming the prior choice of people as health care supplements. The increasing demand for these formulations has led to extensive development of pharmaceutical industries worldwide. The reaction between the preservatives (sodium benzoates and ascorbic acid) used in these formulations could generate benzene. Benzene is classified as class-1 human carcinogen and responsible for various short and long term health effects.

Methods: In this study, 25 formulations (containing ascorbic acid and sodium benzoate) of various manufacturers available as over the counter products were obtained and their benzene content were determined using gas chromatograph with flame ionization detector.

Results: The result showed that 64% of the formulations were free from benzene contamination whereas 36% of formulations were found to be contaminated with benzene. A simple, less time-consuming, economic, and validated gas chromatographic method for estimation of benzene in formulations was also developed successfully in present study.

Conclusions: The data revealed that the level of benzene was within permissible limits, yet the presence of a carcinogen in the marketed formulations intended for internal use is an alarming situation.
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http://dx.doi.org/10.1515/jcim-2020-0012DOI Listing
August 2020

Small Interfering RNAs and RNA Therapeutics in Cardiovascular Diseases.

Adv Exp Med Biol 2020 ;1229:369-381

Multidisciplinary Research Unit, Guru Gobind Singh Medical College, Faridkot, India.

Ribonucleic acid (RNA) is being exploited and understood in its many aspects of function and structure for development of valuable tools in the therapeutics of various diseases such as cardiovascular etc. The expanded knowledge regarding function of RNA in the genomics and inside the cell has dramatically changed the therapeutic strategies in the past few years. RNA has become a spotlight of attention for developing novel therapeutic schemes and hence variety of therapeutic strategies is being coming into the picture that includes RNA interference, use of aptamers, role of microRNA (miRNA) that can alter the complex gene expression patterns. It is due to the fact that RNA offers various advantages in disease management as it can be edited and modified in its various forms such as secondary and tertiary structures. Although scientists are in process of manufacturing RNA-targeting therapies using variety of endogenous gene silencing regulators, Small interfering RNAs (Si RNAs), aptamers and microRNA for cardiovascular diseases yet the development of a novel, risk free therapeutic strategy is a major challenge and need of the hour in cardiovascular medicine. In this regard these agents are required to overcome pleothra of barriers such as stability of drug targets, immunogenicity, adequate binding, targeted delivery etc. to become effective drugs. Recent years have witnessed the progress of RNA therapeutic strategies in cardiovascular diseases that are likely to significantly expand the cardiovascular therapeutic repertoire within the next decade. The present manuscript has been compiled to summarize various approaches of siRNA based therapies in cardiovascular diseases along with the advantages, outcomes and limitations if any in this regard. In addition, the future prospects of RNA therapeutic modalities in cardiovascular diseases are summarized.
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http://dx.doi.org/10.1007/978-981-15-1671-9_23DOI Listing
July 2020

RNA Binding Proteins and Non-coding RNA's in Cardiovascular Diseases.

Adv Exp Med Biol 2020 ;1229:105-118

Multidisciplinary Research Unit, Guru Gobind Singh Medical College, Faridkot, Punjab, India.

Cardiovascular disease (CVD) is the leading cause of mortality as well as morbidity worldwide. The disease has been reported to be chronic in nature and the symptoms of the disease worsen progressively over a long period of time. Inspite of noteworthy achievements have been made in the therapy of CVD yet the available drugs are associated with various undesirable factors including drug toxicity, complexity, resistance and many more. The versatility of RNAs makes them crucial therapeutics candidate for many human diseases. Deeper understanding of RNA biology, exploring new classes of RNA that possess therapeutic potential will help in its successful translation to the clinic. Understanding the mode of action of various RNAs such as miRNA, RNA binding proteins and siRNA in CVD will help in improved therapeutics among patients. Multiple strategies are being planned to determine the future potential of miRNAs to treat a disease. This review embodies the recent work done in the field of miRNA and its role in cardiovascular disease as diagnostic biomarker as well as therapeutic agents. In addition the review highlights the future of miRNAs as a potential therapeutic target and need of designing micronome that may reveal potential predictive targets of miRNA-mRNA interaction.
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http://dx.doi.org/10.1007/978-981-15-1671-9_5DOI Listing
July 2020

Association of CD4/CD8 Ratio with Viral Load, Genotype and Cirrhosis in Chronic Hepatitis C.

J Assoc Physicians India 2020 Feb;68(2):35-38

Professor and Head, Radiodiagnosis, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab.

Abstract: The interplay between Hepatitis C virus (HCV) and immune system, especially T lymphocytes play a major role in the clearance of virus and in development of liver cell injury resulting in replacement of healthy tissue with fibrous scar tissue.

Objectives: To evaluate the association of CD4/CD8 ratio with viral load and genotype of HCV and to evaluate the correlation of CD4/CD8 ratio and CD4 and CD8 cell counts with liver function tests in HCV infected patients.

Methods: Forty patients of Chronic Hepatitis C infection were enrolled for study. Immunophenotyping by flowcytometry for measurement of CD4 and CD8 T cell counts was used and the percentages of cells expressing CD4 and CD8 were estimated per lymphocyte population. HCV viral load quantitative was done by Roche Taqman Method.

Results: The CD4/CD8 ratio was not found to have any significant correlation with HCV viral load. However, it showed a significant difference in the two HCV genotypes, the ratio being higher in genotype 3 than in genotype 1. It showed no significant correlation with liver function tests except serum albumin which had significant positive correlation with CD4/CD8 ratio. The ratio was also found to be significantly decreased in patients with cirrhosis of liver.

Conclusion: Hepatitis C virus genotype but not viral load influences the immune response to HCV infection. The CD4/CD8 ratio significantly decreases in patients with liver cirrhosis than in normal and fatty liver.
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February 2020

Development and Validation of a Novel Stability-Indicating RP-HPLC Method for Simultaneous Determination of Tezacaftor and Ivacaftor in Fixed Dose Combination.

J Chromatogr Sci 2020 Apr;58(4):346-354

Department of Quality Control, Oasis Labs, Singapore, Singapore.

A simple and precise novel stability-indicating method for the simultaneous estimation of tezacaftor and ivacaftor in combined tablet dosage form was developed and validated using reversed-phase high-performance liquid chromatography (RP-HPLC). The method is being reported for the first time and includes an estimation of degradation products produced post-stress conditions without any extraction or derivatization. The chromatographic separation of the drugs was achieved with a Symmetry Shield RP18 Column (100 Å, 5 μm, 4.6 mm × 250 mm) using a mixture of buffer, methanol and acetonitrile (42:27:31 v/v/v) as mobile phase. The buffer used in mobile phase contained 35 mM potassium dihydrogen phosphate, and its pH was adjusted to 7.0 ± 0.02 with 20% orthophosphoric acid. The instrument was set at flow rate of 1.2 mL min-1 at ambient temperature and the wavelength of UV-visible detector at 275 nm. The developed method could be suitable for the quantitative determination of these drugs in pharmaceutical preparations and also for quality control in bulk manufacturing. Stress testing was performed to prove the specificity. No interference was observed from its stress degradation products. The statistical analysis was done by using F-test and t-test at 95% confidence level.
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http://dx.doi.org/10.1093/chromsci/bmz120DOI Listing
April 2020

Bioinformatics-Based Tools and Software in Clinical Research: A New Emerging Area.

Methods Mol Biol 2019 ;1939:215-230

Multidisciplinary Research Unit, Guru Gobind Singh Medical College, Faridkot, India.

Nowadays, drug discovery is a long process which includes target identification, validation, lead optimization, and many other major/minor steps. The huge flow of data has necessitated the need for computational support for collection, storage, retrieval, analysis, and correlation of data sets of complex information. At the beginning of the twentieth century, it was cumbersome to elaborate the experimental findings in the form of clinical outcomes, but current research in the field of bioinformatics clearly shows ongoing unification of experimental findings and clinical outcomes. Bioinformatics has made it easier for researchers to overcome various challenges of time-consuming and expensive procedures of evaluation of safety and efficacy of drugs at a much faster and economic way. In the near future, it may be a major game player and trendsetter for personalized medicine, drug discovery, drug standardization, as well as food products. Due to rapidly increasing commercial interest, currently probiotic-based industries are flooding the market with a range of probiotic products under the banner of dietary supplements, natural health products, food supplements, or functional foods. Most of the consumers are attracted toward probiotic formulations due to the rosy picture provided by the media and advertisements about high beneficial claims. These products are not regulated by pharmaceutical regulatory authorities in different countries of origin and are rather regulated as per their intended use. Lack of stipulated quality standard is a major challenge for probiotic industry; hence there would always be a possibility of marketing of ineffective and unsafe products with false claims. Hence it is very important and pertinent to ensure the safety of probiotic formulations available as over-the-counter (OTC) products for ignorant society. At the same time, probiotic industry, being in its initial stages in developing and underdeveloped countries, requires to ensure safe, swift, and successful usage of probiotics. In the absence of harmonized regulatory guidelines, safety, quality, as well as the efficacy of the probiotic strain does not remain a mandate but becomes a choice for the manufacturer. Hence there is an urgent need to screen already marketed probiotic formulations for their safety with respect to specific strains of probiotic. Various conventional methods used by the manufacturers for the identification of probiotic microbes create a blurred image about their status as probiotics. The present manuscript focuses on a bioinformatics-based technique for validation of marketed probiotic formulation using 16s rRNA sequencing and strain-level identification of bacterial species using Ez Texan and laser gene software. This technique gives a clear picture about the safety of the product for human use.
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http://dx.doi.org/10.1007/978-1-4939-9089-4_12DOI Listing
July 2019

Bioactivity-guided isolation of potent anxiolytic compounds from leaves of .

Ayu 2018 Jan-Mar;39(1):21-28

Department of Herbal Drug Technology, University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, India.

Context: fragrances have been attributed with mood-enhancing properties by aromatherapists. Leaves of this plant have been reported to exert anti-anxiety activity. Till date, no specific phytoconstituent responsible for this has been identified.

Objective: Isolation of anxiolytic constituent of using bioactivity-guided fractionation.

Materials And Methods: Leaf extracts of four varieties of in petroleum ether, chloroform, methanol and water were evaluated for anti-anxiety activity in mice using elevated plus-maze apparatus. Because of activity in methanol extract, it was used for safety evaluation/acute toxicity studies in animals. Bioactive fraction of methanol extract was subjected to column chromatography and structure of the isolated compound was elucidated by melting point, ultraviolet, infrared, nuclear mass reactor and mass spectroscopy. The isolated constituents were further evaluated for anti-anxiety activity using light/dark model and hole-board model of anxiety.

Results: Results showed no mortality at a dose up to 2000 mg/kg body weight that indirectly reflects the safety profile of the leaf extracts. Fractionation of methanol extract led to the isolation of four flavonoids (rutin, quercetin, kaempferol and myricetin). The isolated compounds exhibited significant anxiolytic activity in different animal models.

Conclusion: The study confirms the presence of four flavonoids responsible for anti-anxiety activity.
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http://dx.doi.org/10.4103/ayu.AYU_173_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287406PMC
January 2019

Ethanol content in traditionally fermented ayurvedic formulations: Compromised Good Manufacturing Practice regulations - compromised health.

Am J Drug Alcohol Abuse 2019 14;45(2):208-216. Epub 2018 Nov 14.

a Multidisciplinary Research Unit , University Centre of Excellence in Research, Baba Farid University of Health Sciences , Faridkot , India .

Background: A large part of the population of India prefers the traditional medicine (ayurvedic formulations) for primary health care. However, the effective quality control of herbal medicine is still a big challenge. Numerous reports indicate noncompliance with Compromised Good Manufacturing Practice (GMP) guidelines by the manufacturers which may lead to adverse drug reactions or toxic effects. Asava and arishta are the classical herbal dosage forms wherein fermentation occurs during production leading to the generation of ethanol. The presence of ethanol in these preparations may lead to their misuse. The self-generated ethanol is responsible for extraction of active constituents and acts as a self-preservative. As the procedure for preparation for asava and arishta is same, the ethanol content is also expected to be the same irrespective of the manufacturer.

Objective: The objective of the present study was to assess and compare the ethanol content of some traditionally fermented ayurvedic formulations available in the market.

Method: In this study, 20 formulations from 3 different manufacturers available as over-the-counter products were obtained and their ethanol contents were determined using gas chromatograph with flame ionization detector.

Results: Statistically significant differences were noted in the ethanol content of various ayurvedic formulations. A simple, less time-consuming, economic, and validated gas chromatographic method for estimation of ethanol in fermented ayurvedic formulations was also developed successfully in present study.

Conclusion: The data generated during study reflected poor compliance of GMP guidelines by the manufacturers and hence the quality is being grossly compromised posing a safety hazard.
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http://dx.doi.org/10.1080/00952990.2018.1529181DOI Listing
March 2020

Enasidenib: First Mutant IDH2 Inhibitor for the Treatment of Refractory and Relapsed Acute Myeloid Leukemia.

Anticancer Agents Med Chem 2018 ;18(14):1936-1951

Department of Chemistry, Maharishi Markandeshwar (Deemed to be University), Mullana-133207, Haryana, India.

Background: Acute myeloid leukemia is the collective name for different types of leukemias of myeloid origin affecting blood and bone marrow. The overproduction of immature myeloblasts (white blood cells) is the characteristic feature of AML, thus flooding the bone marrow and reducing its capacity to produce normal blood cells. USFDA on August 1, 2017, approved a drug named Enasidenib formerly known as AG-221 which is being marketed under the name Idhifa to treat R/R AML with IDH2 mutation. The present review depicts the broad profile of enasidenib including various aspects of chemistry, preclinical, clinical studies, pharmacokinetics, mode of action and toxicity studies.

Methods: Various reports and research articles have been referred to summarize different aspects related to chemistry and pharmacokinetics of enasidenib. Clinical data was collected from various recently published clinical reports including clinical trial outcomes.

Result: The various findings of enasidenib revealed that it has been designed to allosterically inhibit mutated IDH2 to treat R/R AML patients. It has also presented good safety and efficacy profile along with 9.3 months overall survival rates of patients in which disease has relapsed. The drug is still under study either in combination or solely to treat hematological malignancies. Molecular modeling studies revealed that enasidenib binds to its target through hydrophobic interaction and hydrogen bonding inside the binding pocket. Enasidenib is found to be associated with certain adverse effects like elevated bilirubin level, diarrhea, differentiation syndrome, decreased potassium and calcium levels, etc.

Conclusion: Enasidenib or AG-221was introduced by FDA as an anticancer agent which was developed as a first in class, a selective allosteric inhibitor of the tumor target i.e. IDH2 for Relapsed or Refractory AML. Phase 1/2 clinical trial of Enasidenib resulted in the overall survival rate of 40.3% with CR of 19.3%. Phase III trial on the Enasidenib is still under process along with another trial to test its potency against other cell lines. Edasidenib is associated with certain adverse effects, which can be reduced by investigators by designing its newer derivatives on the basis of SAR studies. Hence, it may come in the light as a potent lead entity for anticancer treatment in the coming years.
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http://dx.doi.org/10.2174/1871520618666181025091128DOI Listing
July 2019

Method for Detection of miRNAs in Non-Model Organisms with Unreported Database.

Methods Mol Biol 2018 ;1823:197-208

Department of Microbiology, GGSMCH, Faridkot, Punjab, India.

Non-model organisms are studied very frequently, as a simple accessible and convenient system to investigate the role of miRNAs in particular aspect of biology or disease. However, the unavailability of the annotated genome and hence miRNA database of these non-model organisms pose a major constraint for using them more efficiently. Here, we describe a new method to identify miRNAs in non-model organisms without complex sequencing strategies and using miRNAs from close relative organisms as proxy/reference sequences.
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http://dx.doi.org/10.1007/978-1-4939-8624-8_15DOI Listing
March 2019

Targeting miRNA for Therapeutics Using a Micronome Based Method for Identification of miRNA-mRNA Pairs and Validation of Key Regulator miRNA.

Methods Mol Biol 2018 ;1823:185-195

Department of Microbiology, GGSMCH, Faridkot, Punjab, India.

MicroRNAs are 18-22 bp long non-coding sequences and play a critical role in diverse biological processes, through modulation of gene expression at the post-transcriptional level by binding at the 3'-untranslated region of target mRNA. Consequent upon the discovery of structural and functional features of miRNA targeting, several molecular methods have been developed to identify miRNA targets. However, these methods suffer several drawbacks, including technical challenges, requirement of high cell volumes, inability to differentiate between direct and indirect targets, cell/tissue as well as experimental-specificity and imprecise binding site information. Alternatively in silico approach enables the exploration of the potential miRNA-mRNA pairs to investigate signature miRNA and proteins involved in the signaling of various diseases. Here, we describe micronome-based standard method for identification of miRNA-mRNA pairs as well as validation of key regulator miRNA.
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http://dx.doi.org/10.1007/978-1-4939-8624-8_14DOI Listing
March 2019

Ashtawarga plants - Suffering a triple standardization syndrome.

J Tradit Complement Med 2017 Oct 17;7(4):392-399. Epub 2017 Jan 17.

University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, 151203, India.

Ayurveda is one of the oldest known holistic health care systems recommending diverse medicinal uses of plants for prevention and cure of diseases and illness. World Health Organization (WHO) estimates that the holistic system is gaining more popularity due to its easy availability, low cost, congeniality, better accessibility and higher safety than allopathic medicine. Demand of herbal drugs is increasing day-by-day because of increasing popularity of herbal drugs; however market fails to meet this supply due to numerous factors, one of the important factors being the extinction of these plants from local flora. About 560 herbal species of India have been included in the Red List of Threatened species. Hence to overcome problem of non-availability of endangered species, Department of AYUSH, Govt. of India has permitted the substitution of rare herbal drugs with available substitutes on the basis of Ayurvedic concepts. Due to this, herbal drug industry has started exploiting the situation and now Ayurvedic products are suffering from a serious problem of adulteration with addition of spoiled, inferior, spurious drugs that are inferior in therapeutic/chemical properties and used to enhance profits. Adulteration with other plants degrades the quality and credibility of Ayurvedic medicine. Ashtawarga plants being an important part of many Ayurvedic formulations are also available in a very limited amount and likely to be substituted by cheap adulterants. Keeping in view the above situation, a metadata analysis has been conducted to find out types of adulteration/substitutions malpractices going on for Ashtawarga plants.
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http://dx.doi.org/10.1016/j.jtcme.2016.12.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634727PMC
October 2017

Tinospora Cordifolia Induces Cell Cycle Arrest in Human Oral Squamous Cell Carcinoma Cells.

Gulf J Oncolog 2017 May;1(24):10-14

Department of Ocular Biochemistry, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Natural products with medicinal value are gradually gaining importance in clinical research due to their well-known property of no side effects as compared to drugs. Tinospora cordifolia (Guduchi) has been used for centuries in Ayurvedic system of medicine for treating various ailments including cancer. In present study, we found that the Tinospora cordifolia extracts (TCE) induced inhibition of proliferation of KB cells was associated with arrest of G0/G1-phase of cell cycle. The effectiveness of TCE in checking the growth of KB cells without altering the growth of normal peripheral blood mononuclear cells (PBMC) indicates that Tinospora cordifolia has differential effect on normal and malignant cells hence, it may have therapeutic potential in cancer.
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May 2017

Kitchen phytochemicals from Allium cepa - their role in multidrug resistance.

Pak J Pharm Sci 2017 May;30(3):789-792

University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, India.

Bacterial resistance to antibiotics is increasingly becoming a concern to public health. Current antibiotics have failed to bring desirable results to many bacterial infections due to multi-resistant strains. So, concerted efforts are being made to curve this with the help of dietary phytohemicals. A number of dietary phytochemical are being put to trials for antimicrobial activity; however it is worthwhile to search such a part of diet that is very frequently used by major population. Keeping this view in mind an effort has been made to evaluate the antimicrobial activity of most common vegetable component Allium cepa. Methanolic and aqueous extracts were prepared from Allium cepa and antibacterial activity was tested on four pathogenic bacteria (Gram-positive and Gram negative) Staphylococcus aureus, Bacillus Subtilis, Escherichia coli and Pseudomonas aeruginosa using Disc diffusion method and Minimum inhibitory concentration by the Microtitre well plate method. Conventional antibiotic discs of Tetracycline and Gentamycin were used as positive control. Among the two extracts, methanolic extract exhibited a significant antimicrobial activity on the test organisms Ps. aeruginosa (17.5mm) and B. subtilis (11.3mm). The minimum inhibitory concentration value for Ps. aeruginosa was 500μl and for B. subtilis was 250μl for methanolic extract. The growth of other two bacteria i.e. E. coli and S. aureus were not inhibited by methanolic as well as aqueous extract that indicates non susceptibility to aqueous and methanol extract of Allium cepa. From the study, it was concluded that the commonly used Allium cepa possess potent antibacterial property against Bacillus Subtilis and Pseudomonas aeruginosa.
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May 2017

A cross-sectional study: Need of equal respect for all professionals in the Institutional Ethics Committees' composition.

Perspect Clin Res 2017 Apr-Jun;8(2):85-89

Division of Clinical Research, University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, India.

Background: The composition of the Institutional Ethics Committee (IEC) with an equal respect plays a major role in evaluating research proposals to ensure the safety of the subjects and ethical quality of research project. It is mandatory that all research projects with an involvement of human subjects should be approved by the IEC before commencement.

Aim: To find out the equality of respect to members of IEC irrespective of nature of their profession.

Materials And Methods: Methods include a cross-sectional study, the general information and member composition, multidisciplinary nature, independent performance, competence, decision capability, professionally biased of IECs in health research institutions of Punjab, India. A structured self-administered questionnaire was developed and provided to all health/research institutions providing the graduate/postgraduate education in medical sciences under the jurisdiction of Baba Farid University of Health Sciences, Faridkot, Punjab, India.

Results: Of the total 142 institutes, only 80 (56% response rate) institutes responded to the questionnaire. Of the 80 institutes which responded, 65 institutes (81.25%) had IEC, while 15 (18.75%) institutes lacked IEC. The compositions of only 22% IECs were in compliance to Drug Controller General of India (DCGI) guidelines and only nine (14%) institutes of the state of Punjab had approval from the DCGI.

Conclusion: Study clearly indicates the carelessness of regulatory bodies and lack of fair practices toward constitution of the IEC. The ratio of nonscientific members (ethicist, philosopher, and layperson) in the IEC composition was incredibly low. This study also reveals the professionally biased and noncompliance with schedule Y guideline in relation to the representation of lawyers, ethicists, and nonscientific members.
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http://dx.doi.org/10.4103/2229-3485.203041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384405PMC
April 2017

Nutraceuticals in prevention of cataract - An evidence based approach.

Saudi J Ophthalmol 2017 Jan-Mar;31(1):30-37. Epub 2016 Dec 18.

University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, India.

Cataract is a principal cause of blindness in the world and is characterized by clouding of eye's natural lens. Surgery is the major therapeutic step taken to cure cataract; however, it is having its own limitations and complications such as iris prolapse, raised IOP, infection, cystoid macular edema and posterior capsular opacification (PCO). So world is looking toward more robust and natural ways to prevent cataract. One of the important factors that can play a role in prevention of any and many diseases is diet of the people. The inclusion of certain naturally occurring food and nutraceuticals is coming up as a best alternative for curing cataract because of their presumed safety, potential nutritional and therapeutic effects. Some nutraceuticals can act as an anticataract agent through some or the other molecular mechanism if consumed by normal population deliberately or inadvertently.
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http://dx.doi.org/10.1016/j.sjopt.2016.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352946PMC
December 2016

Innate antioxidant activity of some traditional formulations.

J Adv Pharm Technol Res 2017 Jan-Mar;8(1):39-42

Department of Herbal Drug Technology, University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, India.

Herbal medicine is the oldest form of healthcare known to humanity. Recently, much attention is being directed toward the use of antioxidants. There are some very commonly used Ayurvedic preparations that might have inbuilt antioxidant activity, and their therapeutic potential can be partially attributable to its antioxidant activity. Hence, it was proposed to find out antioxidant activity of such common formulations. Estimation of innate antioxidant activity of some commonly used traditional formulations. In this study, five formulations were evaluated for antioxidant activity in comparison to gallic acid (standard) using the reducing power method and superoxide radical scavenging activity by dimethyl sulfoxide method followed by calculation of scavenging activity and inhibitory concentration 50% (IC). The result shows that Ayurvedic drug extracts possess good reducing power and antioxidant activity. shows higher reducing power ranging from 117 ± 0.021 to 0.176 ± 0.012 as compared to other extracts. The drug extracts were also found to be an efficient scavenger of superoxide radical. The IC values for , , , , gallic acid (standard) and , were found to be 50.07, 98.41, 105.13, 116.39, 176.80, and 200.17, respectively. From this study, it can be concluded that the above Ayurvedic formulations possess antioxidant property. However, work could be initiated on the isolation and identification of these antioxidant components.
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http://dx.doi.org/10.4103/2231-4040.197393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5288970PMC
February 2017

Isolation and characterization of quinine from : A new marker approach to identify substitution and adulteration.

J Adv Pharm Technol Res 2016 Oct-Dec;7(4):153-158

Department of Herbal Drug Technology, University Centre of Excellence in Research, Baba Farid University of Health Sciences, Faridkot, Punjab, India.

(Mahameda) is an important ingredient of Ashtawarga and Ayurvedic formulations. Nowadays, it comes under the category of endangered plants due to large scale and indiscriminate collection of wild material. To overcome the scarcity, substitutes of Mahameda are also commonly used in market. These additives are different from the authentic plant by Ayurvedic and pharmacological theory of drug action, thereby resulting in substitution/adulteration. Substitution is a critical issue in isolation and quantification of the therapeutically active ingredients that can be used as markers in the identification of substitution/adulteration. Methanolic extract of the rhizomes of was subjected to preliminary phytochemical screening for the detection of phytoconstituents, followed by column chromatography for isolation of the marker. The column was first eluted with pure hexane, and polarity of the solvent was gradually increased. A total of 1180 fractions were collected and pooled on the basis of thin-layer chromatography profile. The single compound was isolated and confirmed by chemical test, melting point, spectral analysis, and comparison with literature. Phytochemical screening of the extract shows the presence of alkaloids, flavonoids, carbohydrates, terpenoids, and phenolics. A pure white crystalline powder was isolated by column chromatography which was characterized as (6-methoxyquinolin-4-yl-8-vinylquinuclidin-2-yl) methanol, i.e. Quinine. The isolated compound, Quinine, was identified as a novel compound in Mahameda as it has not been reported in the genus , till now. It can be used as a marker for the identification of substitution/adulteration and standardization of .
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http://dx.doi.org/10.4103/2231-4040.191427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052943PMC
November 2016

Micronome revealed miR-19a/b as key regulator of SOCS3 during cancer related inflammation of oral squamous cell carcinoma.

Gene 2016 Dec 28;594(1):30-40. Epub 2016 Aug 28.

Division of Clinical Research, University Centre of Excellence in Research, Baba Farid University of Health Science, Faridkot 151203, Punjab, India. Electronic address:

Although significant advances have been established in molecular biology of Oral squamous cell carcinoma (OSCC), innovative strategies are still required to further understand detailed molecular mechanisms. Using bioinformatic approach, we aim to explore the potential miRNA-mRNA pairs in cancer related inflammatory response and investigate their potential roles as signature miRNA and proteins in the signaling pathway. Firstly, the differentially expressed genes of OSCC were selected which then underwent gene ontology to identify genes engaged in inflammatory response and its regulation. Validated miRNAs were retrieved and miRNAs with complete complementarily with their targets were visualized for miRNA-mRNA regulatory network. Protein-protein interactions of inflammatory and its regulatory genes were analyzed for interacting genes involved in signaling pathway. Eight universal miRNAs were obtained for inflammation and its regulation. miRNA-19a/b showed significant influence in controlling inflammatory response in OSCC. Therefore, micronome on deregulated genes in inflammation identifies miRNA-mRNA pairs which have high potential to be targeted for diagnostic and treatment applications in OSCC.
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http://dx.doi.org/10.1016/j.gene.2016.08.044DOI Listing
December 2016

Emerging role of bioinformatics tools and software in evolution of clinical research.

Perspect Clin Res 2016 Jul-Sep;7(3):115-22

Division of Clinical Research, University Centre of Excellence in Research, Baba Farid University of Health Science, Faridkot, Punjab, India.

Clinical research is making toiling efforts for promotion and wellbeing of the health status of the people. There is a rapid increase in number and severity of diseases like cancer, hepatitis, HIV etc, resulting in high morbidity and mortality. Clinical research involves drug discovery and development whereas clinical trials are performed to establish safety and efficacy of drugs. Drug discovery is a long process starting with the target identification, validation and lead optimization. This is followed by the preclinical trials, intensive clinical trials and eventually post marketing vigilance for drug safety. Softwares and the bioinformatics tools play a great role not only in the drug discovery but also in drug development. It involves the use of informatics in the development of new knowledge pertaining to health and disease, data management during clinical trials and to use clinical data for secondary research. In addition, new technology likes molecular docking, molecular dynamics simulation, proteomics and quantitative structure activity relationship in clinical research results in faster and easier drug discovery process. During the preclinical trials, the software is used for randomization to remove bias and to plan study design. In clinical trials software like electronic data capture, Remote data capture and electronic case report form (eCRF) is used to store the data. eClinical, Oracle clinical are software used for clinical data management and for statistical analysis of the data. After the drug is marketed the safety of a drug could be monitored by drug safety software like Oracle Argus or ARISg. Therefore, softwares are used from the very early stages of drug designing, to drug development, clinical trials and during pharmacovigilance. This review describes different aspects related to application of computers and bioinformatics in drug designing, discovery and development, formulation designing and clinical research.
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http://dx.doi.org/10.4103/2229-3485.184782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936069PMC
July 2016

MicroRNA therapeutics: Discovering novel targets and developing specific therapy.

Perspect Clin Res 2016 Apr-Jun;7(2):68-74

Division of Clinical Research, University Centre of Excellence in Research, Baba Farid University of Health Science, Faridkot, Punjab, India.

MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression in diverse biological process. They act as intracellular mediators that are necessary for various biological processes. MicroRNAs targeting pathways of human disease provide a new and potential powerful candidate for therapeutic intervention against various pathological conditions. Even though, the information about miRNA biology has significantly enriched but we still do not completely understand the mechanism of miRNA gene regulation. Various groups across the globe and pharmaceutical companies are conducting research and developments to explore miRNA based therapy and build a whole new area of miroRNA therapeutics. Consequently, few miRNAs have entered the preclinical and clinical stage and soon might be available in the market for use in humans.
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http://dx.doi.org/10.4103/2229-3485.179431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840794PMC
May 2016

Is There Any Scientific Basis of Hawan to be used in Epilepsy-Prevention/Cure?

J Epilepsy Res 2015 Dec 31;5(2):33-45. Epub 2015 Dec 31.

Baba Farid University of Health Sciences University Centre of Excellence in Research; India.

Epilepsy is a neuropsychiatric disorder associated with religiosity and spirituality. Nasal drug delivery systems are the best for diseases related to brain. In older times RishiMuni, ancient scholars and physicians used to recommend Hawan for mental peace and well being. Gayatri Mantra also tells that sughandhim (aroma, fragrance) puushtivardhanam (gives rise to good health). Om triambkum yajamahe, sughandhim puushtivardhanam, urvarukmev vandhanaat, mrityu mokshay mamritaat! Hawan is a scientific experiment in which special herbs (Hawan Samagri) are offered in the fire of medicinal woods ignited in a specially designed fire pit called agnikuñda. Hawan seems to be designed by the ancient scholars to fight with the diseases of the brain. Our metadata analysis demonstrates that the components of Hawan are having a number of volatile oils that are specifically useful for epilepsy through one or the other mechanism of action. Due to high temperature of fire the vapors of these oils enter into the central nervous system through nasal route. The routine of performing Hawan might keep the threshold value of the therapeutic components in the body and help in preventing epilepsy. In the present manuscript authors have tried to highlight and integrate the modern and ancient concepts for treatment and prevention of epilepsy.
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http://dx.doi.org/10.14581/jer.15009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4724851PMC
December 2015

Inferior rectus muscle ocular cysticercosis: A case report.

Saudi J Ophthalmol 2015 Apr-Jun;29(2):175-7. Epub 2014 Nov 21.

Department of Ocular Biochemistry, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Cysticercosis is a systemic parasitic disease caused by the larval form of cestode Taenia solium. It has a worldwide distribution and is potentially harmful with variable clinical manifestations. The most commonly involved sites include eye, brain, bladder wall, and heart. Ocular cysticercosis can be extraocular or intraocular and may present with varied clinical symptoms. We report the condition in a thirteen year old female child who presented with mild lower lid swelling and diplopia in upgaze, wherein cysticercus cellulosae cyst was found within the mass of the right inferior rectus muscle. It becomes important to report this case because of the relative rarity of the condition these days, unusual site of the cyst and the young age of the patient.
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http://dx.doi.org/10.1016/j.sjopt.2014.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4398793PMC
April 2015

Algal lectins as promising biomolecules for biomedical research.

Crit Rev Microbiol 2015 Feb 16;41(1):77-88. Epub 2013 Jul 16.

Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University , Patiala, Punjab , India and.

Lectins are natural bioactive ubiquitous proteins or glycoproteins of non-immune response that bind reversibly to glycans of glycoproteins, glycolipids and polysaccharides possessing at least one non-catalytic domain causing agglutination. Some of them consist of several carbohydrate-binding domains which endow them with the properties of cell agglutination or precipitation of glycoconjugates. Lectins are rampant in nature from plants, animals and microorganisms. Among microorganisms, algae are the potent source of lectins with unique properties specifically from red algae. The demand of peculiar and neoteric biologically active substances has intensified the developments on isolation and biomedical applications of new algal lectins. Comprehensively, algal lectins are used in biomedical research for antiviral, antinociceptive, anti-inflammatory, anti-tumor activities, etc. and in pharmaceutics for the fabrication of cost-effective protein expression systems and nutraceutics. In this review, an attempt has been made to collate the information on various biomedical applications of algal lectins.
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http://dx.doi.org/10.3109/1040841X.2013.798780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113906PMC
February 2015

In vitro evaluation of antimicrobial activity of Kutajghan vati - an Ayurvedic formulation.

Pak J Pharm Sci 2012 Jul;25(3):693-6

School of Pharmaceutical Sciences, Shobhit University, Meerut, India.

The present investigation focuses to determine the antimicrobial potential of an Ayurvedic formulation Kutajghan vati. In this study the activity of this formulation was compared with the standard antibiotics like Amikacin and Norfloxacin. Ethanol, methanol and acetone extract of Kutajghan vati demonstrated good antimicrobial activity and thus can form the basis for the development of a novel antibacterial formulation.
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July 2012

Estimation of heavy metals in commonly used medicinal plants: a market basket survey.

Environ Monit Assess 2010 Nov 18;170(1-4):657-60. Epub 2009 Dec 18.

National Institute of Ayurvedic Pharmaceutical Research, Moti Bagh Road, Patiala, 147001, Punjab, India.

Popularity of herbal drugs is increasing all over the world because of lesser side effects as compared to synthetic drugs besides it cost effectiveness and easy availability to poor people particularly in developing countries. Keeping in view the increased market demand of herbal drugs, it is essential to ensure their chemical quality prior to use. Raw drugs and herbs are usually collected from different places, which might be contaminated with various contaminants. It is pertinent to estimate the levels of heavy metals and other micronutrients, which could be affected by their presence in the surrounding environments. Heavy metals are known to pose a potential threat to terrestrial and aquatic biota. Keeping this in view, samples of ten plants or plant parts used in drug making were collected from local markets of Punjab for heavy metal and micronutrient estimation. It was found that the samples were contaminated having cadmium, lead, chromium, iron, manganese, and zinc. The highest mean level of cadmium (23.1 μg/kg) was found in Haritaki sample. Chromium concentration of the plant samples ranged between 7.25 and 1.34 μg/kg with the highest values was in Daruharidra and lowest in Pippali. The levels of these heavy metals were within permissible limits.
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http://dx.doi.org/10.1007/s10661-009-1264-3DOI Listing
November 2010
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