Publications by authors named "Pandurengan Padmavathy"

2 Publications

  • Page 1 of 1

Effect of triclosan (TCS) on the protein content and associated histological changes on tilapia, Oreochromis mossambicus (Peters, 1852).

Environ Sci Pollut Res Int 2021 Nov 20;28(42):59899-59907. Epub 2021 Jun 20.

Department of Fisheries Biology and Resource Management, Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University (TNJFU), Thoothukudi, Tamil Nadu, 628 008, India.

Triclosan is a chlorinated phenolic antimicrobial agent having a wide application in commercial and healthcare products. The toxic effects of the emerging pollutant, triclosan (TCS), on behavior, protein content, and associated histological alterations in the muscle tissue of the freshwater fish Oreochromis mossambicus were studied. Healthy fishes were exposed to five different acute concentrations of TCS, viz., 0.131, 0.262, 0.523, 1.046, and 2.092 ppm for a period of 96 h. The 96-h LC of TCS for O. mossambicus was determined as 0.715 ppm using probit analysis. The fishes were also chronically exposed to the five different concentrations of TCS based on LC to study the toxic effects of long-term exposure. The protein content of the fish muscle gradually decreased with an increase in the concentration of TCS. Further, the histological alterations such as splitting of myotomes, vacuolar degeneration, degenerated myotomes, multifocal degeneration of myocytes, degeneration of myoepithelium, myolysis, melanomacrophage in the dermis, vacuolation in the epidermis, atrophy of myotomes, and necrosis were observed during the acute and chronic exposure of fishes to TCS. The study revealed that TCS can affect the aquatic organisms even at a minimum concentration of 0.715 ppm causing changes in the behavior and biochemical constituents of tilapia.
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November 2021

A single enzyme PCR-RFLP protocol targeting 16S rRNA/tRNA region to authenticate four commercially important shrimp species in India.

Food Chem 2018 Jan 23;239:369-376. Epub 2017 Jun 23.

Department of Fisheries Environment, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukkudi 628 008, India.

Food authenticity is an issue of major concern for food authorities, as mislabeling represents one of the major commercial frauds. In this study, a novel PCR-RFLP protocol was developed as a tool to authenticate four shrimp products of commercial importance belonging to the family, Penaeidae, viz. Litopenaeus vannamei, Penaeus monodon, P. semisulcatus and Fenneropenaeus indicus. PCR amplification was performed targeting 16S rRNA/tRNA region having an amplicon size of 530bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3. Subsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded distinct RFLP pattern for each species of shrimps having fragment sizes below 150bp. The unique RFLP patterns were also obtained in processed shrimp products without any degradation or alteration in the major fragments. The method was also validated with commercial shrimp products. Thus, the developed protocol can be performed within 8h using a single enzyme to authenticate four shrimp products of commercial significance.
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January 2018