Publications by authors named "P Manigandan"

5 Publications

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Tea polyphenol-T. arjuna bark as sperm antioxidant extender in infertile smokers.

Cryo Letters 2017 Mar/Apr;38(2):95-99

Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.

Background: Antioxidants protect spermatozoa against lipid peroxidation during freezing.

Objective: The study is designed to elucidate the suitable extender to preserve infertile semen of smokers against ROS damage using natural Tea polyphenol (T. arjuna bark extract).

Materials And Methods: Forty-two infertile subjects with smoking habit and 28 fertile subjects without smoking habit were considered for the study. Four semen extenders including our naturally derived antioxidant component were prepared and used to preserve semen sample from the study subjects for a period of one month. Standard semen parameters, biochemical and sperm DNA damage marker with inhibition were measured before and after cryopreservation.

Results: The motility and morphology of sperm cells were maintained better in E4 extender, and DNA damage is reduced.

Conclusion: Extender recipe with natural antioxidants (E3 and E4) was found to be apt for infertile semen preservation.
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July 2017

Uptake of some radionuclides by woody plants growing in the rainforest of Western Ghats in India.

J Environ Radioact 2014 Apr 23;130:63-7. Epub 2014 Jan 23.

Kongunadu Arts and Science College (Bharathiar University), Coimbatore, India. Electronic address:

Transfer of the naturally occurring radionuclides (238)U, (232)Th, and (40)K, and the fallout radionuclide (210)Po to different wild plant species in the rainforest of Western Ghats was analyzed. A number of physiologically different plants from the top storey and understorey, such as shrubs and epiphytes, were compared. The concentrations of these radionuclides in the plants and soil were measured using a gamma ray spectrometer and an alpha counter, and were found to vary widely within plants and between species. The soil-plant ratios also varied between species while Elaeocarpus oblongus and epiphytic plants exhibited preferential uptake of these radionuclides. As a result, the dust particles trapped in the root systems of epiphytes could be used as bioindicators of fallout radionuclides in the Western Ghats.
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http://dx.doi.org/10.1016/j.jenvrad.2013.12.023DOI Listing
April 2014

Determination of perfluorooctanoate and perfluorooctanesulfonate in water matrices by inline matrix elimination liquid chromatography with reversed phase separation and suppressed conductivity detection.

J Chromatogr Sci 2011 Sep;49(8):603-9

Metrohm India Limited, 13, First Avenue, Indira Nagar, Adyar, Chennai 600 020, Tamilnadu, India.

This work describes a new method for the determination of perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) in water matrices by suppressed conductivity detection. Separation was achieved by isocratic elution on a reversed-phase column thermostated at 45°C using an aqueous mobile phase containing boric acid and acetonitrile. The PFOA and PFOS content in the water matrix were quantified by a pre-concentration technique. For the concentration range of 1 to 15 ng/mL and 2 to 30 ng/mL, the linear calibration curve for PFOA and PFOS yielded coefficients of determination (R(2)) of 0.9995 and 0.9985, respectively. The relative standard deviations were smaller than 1.5% for PFOA and PFOS. The retention-time precision of four consecutive 12 h injections was smaller than 0.641% and 0.818%, respectively. The presence of common divalent cations, such as calcium, magnesium, and iron in water matrices impairs PFOS recovery. This drawback was overcome by applying inline matrix elimination method. The optimized method was successfully applied for drinking water, ground water, and seawater samples.
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http://dx.doi.org/10.1093/chrsci/49.8.603DOI Listing
September 2011

An improved ion chromatographic method for fast and sensitive determination of N-methylpyrrolidine in cefepime hydrochloride.

J Chromatogr Sci 2009 Aug;47(7):549-52

Metrohm India Limited, Application Lab.

An alternative ion chromatographic method to the existing USP method for the determination of N-methylpyrrolidine (NMP) in cefepime hydrochloride is developed. The cefepime in solution behaves as a strong cation and gets retained in the analytical column, leading to reduction in column capacity and irreproducible retention time. The retained drug has to be removed with a special rinsing solution, followed by re-equilibration with the mobile phase. This process takes at least 3 to 4 h time for sample analysis. We used a silica-based cation exchange column with poly-butadiene-maleic acid functional group attached with an optimized mobile phase composition. The characteristic feature of this method is the short analysis time with a clear separation of NMP and the cationic drug molecule within a run-time of 30 min. The developed method overcomes the limitations of the USP method. This method describes the matrix elimination by choosing appropriate column and eluent condition. The method is tested for selectivity, linearity, limits of detection and quantification, accuracy, and precision and is suitable for continuous sample analysis.
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http://dx.doi.org/10.1093/chromsci/47.7.549DOI Listing
August 2009

Ion chromatographic determination of residual phase transfer catalyst in active pharmaceutical ingredient.

J Chromatogr Sci 2009 Aug;47(7):540-4

Metrohm India Limited, Application Lab.

A new ion chromatography method with non-suppressed conductivity detection has been developed for the quantification of residual phase transfer catalyst-tetrabutylammonium bromide (TBAB) in an active pharmaceutical drug, Levetiracetam. Separation conditions are optimized to get a clear separation of TBAB from drug impurities using a Metrosep Cation C2-150 column. Conditions are also optimized to separate tetramethylammonium bromide, tetraethylammonium bromide, and tetrapropylammonium bromide, which are also used as phase transfer catalysts in several syntheses. Method performance was checked for selectivity, linearity, limit of quantification, limit of detection, accuracy, and precision. The method has superior performance with linearity r(2) > or = 0.9999, recovery from 94.7% to 96.5%, precision < or = 0.74%. In-line preconcentration is used to achieve limits of detection and quantification of 39 ng and 118 ng of TBAB, which corresponds to 1.56 and 4.72 microg/g of TBAB with respect to sample weight. The proposed method can be used for routine quality assurance analysis in the pharmaceutical industry.
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http://dx.doi.org/10.1093/chromsci/47.7.540DOI Listing
August 2009