Publications by authors named "P Lopez"

1,543 Publications

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Aggressive multiple sclerosis in Argentina: Data from the nationwide registry RelevarEM.

J Clin Neurosci 2021 Jun 1. Epub 2021 Jun 1.

Neuroimmunology Unit, Department of Neuroscience, Hospital Aleman, Buenos Aires, Argentina.

The objectives of the present study were to describe the frequency of aggressive multiple sclerosis (aMS) as well as to compare clinical and radiological characteristics in aMS and non-aMS patients included in RelevarEM (NCT03375177).

Methods: The eligible study population and cohort selection included adult-onset patients (≥18 years) with definite MS. AMS were defined as those reaching confirmed EDSS ≥ 6 within 5 years from symptom onset. Confirmation was achieved when a subsequent EDSS ≥ 6 was recorded at least six months later but within 5 years of the first clinical presentation. AMS and non-aMS were compared using the χ2 test for categorical and the Mann-Whitney for continuous variables at MS onset and multivariable analysis was performed using forward stepwise logistic regression with baseline characteristics at disease onset.

Results: A total of 2158 patients with MS were included: 74 aMS and 2084 non-aMS. The prevalence of aMS in our cohort was 3.4% (95%CI 2.7-4.2). AMS were more likely to be male (p = 0.003), older at MS onset (p < 0.001), have primary progressive MS (PPMS) phenotype (p = 0.03), multifocal presentation (p < 0.001), and spinal cord as well as infratentorial lesions at MRI during disease onset (p = 0.004 and p = 0.002, respectively).

Conclusion: 3.4% of our patient population could be considered aMS. Men, patients older at symptom onset, multifocal presentation, PPMS phenotype, and spinal cord as well as brainstem lesions on MRI at clinical presentation all had higher odds of having aMS.
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http://dx.doi.org/10.1016/j.jocn.2021.05.047DOI Listing
June 2021

Headache as main reason for consultation to a hospital Emergency Department in Spain: a prospective study.

Neurologia 2021 May 31. Epub 2021 May 31.

Servicio de Neurología, Hospital Universitario Marqués de Valdecilla e IDIVAL y Universidad de Cantabria, Santander, España. Electronic address:

Introduction: Headache is common in the general population and a frequent reason for medical consultation.

Objectives: To describe the characteristics of patients attending the Emergency Department (ED) for headache.

Methods: A descriptive study with prospective collection of 100 consecutive patients over 15 years old who attended our ED due to headache as the main complaint.

Results: Headache accounted for 1,4% of ED visits. The most common age range is between 31 and 45 years and the majority of the patients are females (61%). We diagnosed 67 primary and 33 secondary headaches. The most frequent diagnosis was migraine, with 36% of cases. One out of 3 patients had a history of headache and 4 out of 5 consulted by their own decision. Only a small percentage of patients were admitted as inpatients (12%), and 3 out of 5 were referred to Primary Care. Complementary tests were performed on 84% of the patients. One CT scan was performed for every 3 patients. A total of 80% patients was correctly diagnosed by the ED physicians.

Conclusions: Headache is a frequent complaint in the ED, where primary headaches are the most common with migraine being the most frequent reason for consultation. In our setting, there is a good screening and diagnosis of headaches, as well as an adequate use of the available resources in the ED for their diagnosis and management.
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http://dx.doi.org/10.1016/j.nrl.2021.03.015DOI Listing
May 2021

Comparing competing geospatial measures to capture the relationship between the neighborhood food environment and diet.

Ann Epidemiol 2021 May 26. Epub 2021 May 26.

Department of Population Health, New York University Grossman School of Medicine, New York, NY; Wagner Graduate School of Public Service, New York University, New York, NY.

Purpose: To examine how the choice of neighborhood food environment definition impacts the association with diet.

Methods: Using food frequency questionnaire data from the Reasons for Geographic and Racial Differences in Stroke study at baseline (2003-2007), we calculated participants' dietary inflammation score (DIS) (n=20,331); higher scores indicate greater pro-inflammatory exposure. We characterized availability of supermarkets and fast food restaurants using several geospatial measures, including density (i.e., counts/km) and relative measures (i.e., percentage of all food stores or restaurants); and various buffer distances, including administrative units (census tract) and empirically-derived buffers ("classic" network, "sausage" network) tailored to community type (higher-density urban, lower-density urban, suburban/small town, rural). Using generalized estimating equations, we estimated the association between each geospatial measure and DIS, controlling for individual- and neighborhood-level sociodemographics.

Results: The choice of buffer-based measure did not change the direction or magnitude of associations with DIS. Effect estimates derived from administrative units were smaller than those derived from tailored empirically-derived buffer measures. Substantively, a 10% increase in the percentage of fast food restaurants using a "classic" network buffer was associated with a 6.3 (SE=1.17) point higher DIS (p<0.001). The relationship between the percentage of supermarkets and DIS, however, was null. We observed high correlation coefficients between buffer-based density measures of supermarkets and fast food restaurants (r=0.73-0.83), which made it difficult to estimate independent associations by food outlet type.

Conclusions: Researchers should tailor buffer-based measures to community type in future studies, and carefully consider the theoretical and statistical implications for choosing relative (vs. absolute) measures.
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http://dx.doi.org/10.1016/j.annepidem.2021.05.005DOI Listing
May 2021

inhibitory effect of Lam. extracts over and on different stages of the chlamydial life cycle.

Heliyon 2021 May 18;7(5):e06947. Epub 2021 May 18.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Microbiología Clínica, Ciudad Autónoma de Buenos Aires, Argentina.

Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both and can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method in order to detect antichlamydial activity of different extracts obtained from native Argentinian plants used as antimicrobials in local ethnomedicine and to evaluate their inhibitory activity over and growth. The inhibitory activity over different stages of the chlamydial life cycle on cell culture was assessed: the entry, the inclusion developing after entry, and the exponential growth stage. Also, the capability of rendering the cell refractory to chlamydial infection by pre-incubation with the extracts was assayed. Inhibitory activity of water-based and organic-based extracts obtained from Lam. (Araliaceae), (Vell.) Engl. (Anacardiaceae) and (Mutis ex L.f.) Baill. (Violaceae) were tested against five strains of (L2/434/BU and four clinical isolates form both neonatal conjunctivitis and adult genital infections, genotypes D, E, and K) and against AR39. The dichloromethane extract showed a broad inhibitory activity over the exponential growth stage of and independently from the chlamydial strain and the cell line. These results suggest a high inhibitory potential on both species. In order to characterize the dichloromethane active extract, an H-NMR was performed. The H-NMR characterization showed a spectrum with characteristic signals of the fatty acid moiety of lipids or cerebrosides, volatile phenolics, phytosterols, methyl triterpenes signals, and glucose moiety of the cerebrosides.
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http://dx.doi.org/10.1016/j.heliyon.2021.e06947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141889PMC
May 2021

Sheath fluid impacts the depletion of cellular metabolites in cells afflicted by sorting induced cellular stress (SICS).

Cytometry A 2021 May 24. Epub 2021 May 24.

Cytometry and Cell Sorting Laboratory, NYU Langone Health, New York City, New York, USA.

Flow cytometrists have long observed a spectrum of cell-type-specific changes ranging from minor functional defects to outright cell destruction after purification of cells using conventional droplet cell sorters. We have described this spectrum of cell perturbations as sorter induced cellular stress, or SICS (Lopez and Hulspas, Cytometry, 2020, 97, 105-106). Despite the potential impact of this issue and ubiquitous anecdotes, little has been reported about this phenomenon in the literature, and the underlying mechanism has been elusive. Inspired by others' observations (Llufrio et al., Redox Biology, 2018, 16, 381-387 and Binek et al., Journal of Proteome Research, 2019, 18, 169-181), we set out to examine SICS at the metabolic level and use this information to propose a working model. Using representative suspension (Jurkat) and adherent (NIH/3T3) cell lines we observed broad and consistent metabolic perturbations after sorting using a high-speed droplet cell sorter. Our results suggest that the SICS metabolic phenotype is a common cell-type-independent manifestation and may be the harbinger of a wide-range of functional defects either directly related to metabolism, or cell stress response pathways. We further demonstrate a proof of concept that a modification to the fluidic environment (complete media used as sheath fluid) in a droplet cell sorter can largely rescue the intracellular markers of SICS, and that this rescue is not due to a contribution of metabolites found in media. Future studies will focus on characterizing the potential electro-physical mechanisms inherent to the droplet cell sorting process to determine the major contributors to the SICS mechanism.
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http://dx.doi.org/10.1002/cyto.a.24361DOI Listing
May 2021