Publications by authors named "Osman Caglayan"

55 Publications

Prevention of the harmful effects of free oxygen radicals by using N-acetylcysteine in testicular torsion.

J Pediatr Urol 2020 Feb 6;16(1):42.e1-42.e8. Epub 2019 Nov 6.

Department of Pathology, Healthy Sciences University, Numune Education and Research Hospital, Ankara, Turkey.

Introduction: Testicular torsion is a urological emergency both in childhood and in adult life. Many studies on experimental testicular torsion have demonstrated biochemical and pathological ischemia-reperfusion injury and the efficacy of some drugs have been investigated to prevent this damage. N-acetylcysteine (NAC) promotes glutathione synthesis and acts as a glutathione precursor because of the fact that it increases the glutathione-reductase activity by transporting sulfhydryl groups.

Aim: In this experimental study, the authors aimed to investigate the effectiveness of NAC in preventing ischemia-reperfusion injury following testicular torsion and detorsion.

Study Design: For this experimental study, 36 albino Wistar-male rats were used. The rats were randomly divided into 4 groups: sham (n = 8), ischemia-reperfusion (n = 8), ischemia-NAC -reperfusion (n = 10), and ischemia-NAC-reperfusion-NAC (n = 10) groups. Two hours of torsion and 4 h of detorsion were created in the left testis. After 4 h of detorsion, the rats were sacrificed. Each tissue was divided into two sections for biochemical and pathological examinations.

Results: There was a statistically significant difference between the study groups in terms of the total-sulfhydryl level, nitric oxide level, and the malondialdehyde values. Histopathological examination revealed that NAC was effective in preventing reperfusion injury in the testis but ineffective in preventing the reduction in the spermatid count.

Discussion: The results of this experimental study support that NAC can histopathologically maintain the structure of seminiferous tubules against ischemis reperfusion injury and prevent damage to the germinative cells. However, it was unable to prevent the reduction in spermatid count. There was no significant difference in the prevention of ischemia-reperfusion injury between NAC administration during the first hour of ischemia and NAC administration during reperfusion. Although NAC can prevent tissue damage from ischemia reperfusion injury, it is not effective against the reduction in the spermatid count.

Conclusion: N-acetylcysteine may be biochemically effective in preventing ischemia-reperfusion injury after testicular torsion and detorsion. NAC is a readily available and easy to use agent that can be used during testicular ischemia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpurol.2019.10.028DOI Listing
February 2020

The Effect of Simvastatin on Pulmonary Damage in Experimental Peritonitis in Rats.

Indian J Surg 2015 Dec 1;77(Suppl 2):370-5. Epub 2013 Feb 1.

Departments of General Surgery, Kırıkkale University School of Medicine, Kırıkkale, Turkey.

Statins are widely used in the treatment of hyperlipidemia, as they inhibit cholesterol synthesis. They also have anti-inflammatory, antioxidant, immunomodulatory, and positive endothelial-functional effects. It is hypothesized that simvastatin ameliorates pulmonary damage secondary to peritonitis in rats. Forty Wistar albino rats were divided into four groups. In sham group, laparotomy was the standard procedure. In simvastatin group, simvastatin was given perorally before laparotomy. In sepsis group, peritoneal sepsis was constituted by cecal ligation and puncture technique. In sepsis + simvastatin group, the procedures of simvastatin and sepsis groups were applied together. After sacrification at the 72nd hour, tissue samples from lungs were harvested for histopathological examination, wet and dry weight measurements, and tissue culture, tissue malondialdehyde, and nitric oxide tests. Blood samples were taken for C-reactive protein and whole blood count. While the malondialdehyde levels were found to be significantly higher in sepsis group, nitric oxide levels were found to be significantly lower in simvastatin + sepsis group. Alveolar hemorrhage was highest in simvastatin + sepsis group. There was no difference for C-reactive protein, leukocyte levels, and histopathological examination between any groups. The ratios of wet and dry lung weights were higher in simvastatin-given groups. Simvastatin has no positive effect in terms of lung dysfunction on experimental sepsis model. For a better understanding of the effects of simvastatin on lung injury in peritoneal sepsis, experimental models of longer duration that enable to search the effects of simvastatin beyond 3 days will be more useful.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12262-013-0845-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692947PMC
December 2015

Acute and chronic impact of smoking on salivary and serum total antioxidant capacity.

J Pak Med Assoc 2015 Feb;65(2):164-9

Objective: To investigate increased oxidative stress in saliva of smokers along with their serum.

Methods: The case-control study was conducted from September to December 2008 in the Department of Biochemistry of the Medical School, Kirikkale University, Kirikkale, Turkey. A blood sample and saliva samples before and after smoking were collected from the smokers, while blood and saliva samples were taken from the controls. All samples were taken concurrently. The samples were measured for total antioxidant capacity, total oxidant stress, oxidative stress index, malondialdehyde, nitric oxide and total sulfhydryl groups. Levels of superoxide dismutase and glutathione peroxidase were also measured on saliva samples. SPSS 13 was used for statistical analysis.

Results: Of the 54 subjects in the study, 27(50%) were smokers with a mean age of 28.4 ± 5.42 years, and 27 (50%) were controls with a mean age of 29.7 ± 8.03 years. Total oxidant stress, oxidative stress index, malondialdehyde and nitric oxide levels were found higher in the serum samples of smokers (p < 0.05), and the levels of total sulfhydryl groups in smokers were lower compared with the controls (p < 0.05). Among the smokers, salivary malondialdehyde levels were higher before and after smoking (p < 0.05), glutathione peroxidase levels were lower than the controls, and salivary nitric oxide levels after smoking were higher than both those of the control group and the levels before smoking (p < 0.05).

Conclusion: Both acute and chronic increased oxidative conditions may be a significant sign of the destructive effects of smoking. The investigation of disorders in smokers concerning oxidative stress will be beneficial in terms of novel approaches and treatment modalities.
View Article and Find Full Text PDF

Download full-text PDF

Source
February 2015

Prevention of retrosternal pericardial adhesions after cardiac surgery with mitomycin C.

Heart Lung Circ 2014 Apr 23;23(4):357-62. Epub 2013 Oct 23.

Necmettin Erbakan University, Meram Medical School, Dept. of Cardiovascular Surgery, Konya, Turkey.

Background: Retrosternal pericardial adhesions may cause significant injuries to the heart and great vessels in cases that require reoperation. Fibroblast proliferation is one of the mechanisms for adhesion formation. Inhibition of fibroblast proliferation is important in reduction of retrosternal adhesions. Mitomycin C (MMC) is able to reduce fibroblast proliferation. We aimed to determine the effect of MMC on prevention of retrosternal pericardial adhesions after primary cardiac operations.

Methods: Thirty Sprague-Dawley female rats were used in the study. The rats were divided into two groups. First group was control (n=10) and second group was MMC study group (n=20). Xiphoid cartilage resection and retrosternal abrasion via subxiphoidal incision was performed. In the study group, MMC (1mg/kg) was topically applied to the retrosternal space. After 15 days, rats were taken into reoperation and adhesion was graded. Tissue and blood samples were taken before termination procedure. Standard staining procedures and fibroblast growth factor receptor 3 antibody staining, were applied immunohistochemically to tissue samples.

Results: The average adhesion scores of the control (n=10) and study (n=20) (MMC; 1mg/kg) groups were 2.50 ± 1.27 and 0.70 ± 0.86, respectively. The adhesion score of the study group was lower than the control group (p<0.05). Immunohistochemical samples revealed that tissue fibroblast intensity was significantly higher in the control group than the study group (p<0.05). There was no statistical significance between two groups in case of hydroxyproline levels (p>0.05).

Conclusion: MMC was found to be effective in the prevention of retrosternal pericardial adhesions without any delay in normal tissue regeneration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.hlc.2013.10.080DOI Listing
April 2014

Can resistin be a new indicator of neonatal sepsis?

Pediatr Neonatol 2014 Feb 29;55(1):53-7. Epub 2013 Jun 29.

Zeynep Kamil Maternity and Children's Education and Training Hospital, NICU, Istanbul, Turkey.

Background: Sepsis is an important cause of neonatal death and perinatal brain damage, particularly in preterm infants. It is thought that activation of the inflammatory cascade triggered by cytokine might play a role in the pathogenesis of sepsis. Recent evidence supports a role for resistin in inflammation. There are no data in the literature on resistin levels of premature newborns with sepsis, which can also cause inflammatory response. The objective of this study was to evaluate whether resistin can be used as an indicator in neonatal sepsis of preterm babies.

Materials And Methods: Forty-three premature newborns considered to have sepsis were included in the study. Forty-three gestational and postnatal age- and sex-matched premature newborns without premature prolonged rupture of membrane or sepsis served as controls.

Results: The median resistin and interleukin-6 (IL-6) levels of the premature babies with sepsis were 85.9 ng/mL and 342.7 pg/mL, respectively, and were higher than those of the control group (29.9 ng/mL and 17.7 pg/mL, respectively). The sensitivity, specificity, positive, and negative predictive values for resistin were 73.7%, 45.8%, 68.3%, and 52.4%, respectively.

Conclusion: Resistin levels were higher in premature newborns with sepsis and correlated with IL-6 levels, which is an indicator of neonatal sepsis. This suggests that resistin may also be used in the diagnosis of neonatal sepsis. However, it has limited value when compared with the other inflammatory markers including C-reactive protein, procalcitonin, and IL-6.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pedneo.2013.04.012DOI Listing
February 2014

Plasma apelin levels in patients with polycystic ovary syndrome.

J Turk Ger Gynecol Assoc 2012 1;13(1):27-31. Epub 2012 Mar 1.

Specialist of Obstetric and Gynecology, Sakarya Education and Research Hospital, Sakarya, Turkey.

Objective: The aim of the study was to evaluate plasma apelin levels in patients with polycystic ovary syndrome (PCOS) and healthy controls.

Material And Methods: Plasma apelin levels, serum lipid levels, serum hormone levels, and homeostasis model assessment-insulin resistance (HOMA-IR) values of 32 patients with PCOS and 31 healthy women forming the control group were checked.

Results: Plasma apelin levels of the PCOS group (0.350±0.083 ng/ml) were significantly higher than those of the control group (0.246±0.045 ng/ml) (p<0.001). No significant correlation was detected between apelin levels and biochemical or clinical data in PCOS group.

Conclusion: Plasma apelin levels were significantly higher in PCOS patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5152/jtgga.2011.74DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940220PMC
March 2014

Total and lipid bound sialic acid levels in patients with polycystic ovary syndrome.

J Turk Ger Gynecol Assoc 2012 1;13(2):79-84. Epub 2012 Jun 1.

Department of Biochemistry and Clinical Biochemistry, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey.

Objective: To evaluate serum total and lipid bound sialic acid (TSA&LBSA) levels in patients with polycystic ovary syndrome (PCOS).

Material And Methods: Forty women with PCOS and 35 healthy controls were enrolled in the study. Serum TSA, LBSA, follicle stimulating hormone, lutenizing hormone, estradiol, thyroid stimulating hormone, prolactin, dehydroepiandrosterone sulphate, androstenedione, free testosterone, total testosterone, 17-OH progesterone, sex hormone binding globulin, cortisol, total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein were measured in each subject. Insulin resistance was estimated by fasting insulin level, fasting glucose: insulin ratio and 75-g glucose tolerance test for 2 hours.

Results: Serum TSA levels were not significantly different between the groups. Serum LBSA levels were higher in patients with PCOS compared to the control group. TSA was correlated with androstenedione and HOMA-IR in the PCOS group. Positive correlations were found between LBSA and dehydroepiandrosterone sulphate in patients with PCOS. After correction for BMI, the only existing significant correlation was between LBSA and follicle stimulating hormone.

Conclusion: Serum LBSA levels, which has previously been found to be higher in cardiovascular diseases and diabetes mellitus, are elevated in PCOS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5152/jtgga.2012.08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939142PMC
March 2014

The value of CRP, IL-6, leptin, cortisol, and peritoneal caspase-3 monitoring in the operative strategy of secondary peritonitis.

Ulus Travma Acil Cerrahi Derg 2011 Sep;17(5):390-5

Department of General Surgery, Kırıkkkale University Faculty of Medicine, Kırıkkale, Turkey.

Background: We aimed to investigate the impact of C-reactive protein (CRP), interleukin (IL)-6, leptin, cortisol, and caspase-3 on the decision of terminating planned abdominal repair in secondary peritonitis.

Methods: Fifteen patients with peritonitis were enrolled into the study. Serum CRP, IL-6, leptin, cortisol, and peritoneal caspase-3 activities were measured.

Results: APACHE II scores at 48 hours (h) and age were significantly higher in non-survivors. A significant decrease was observed in caspase-3 activities of patients in whom ≤4 laparotomies were performed when compared with those who underwent >4 laparotomies. For patients who underwent ≤4 laparotomies, there was a significant difference in caspase-3 levels between 0 and 72 h. There was no significant difference in caspase-3 levels in non-survivors; caspase-3 levels were significantly lower in the survivors at 48 and 72 h. Changes in CRP, IL-6, leptin, and cortisol levels were not statistically significant.

Conclusion: CRP, IL-6, leptin, cortisol, and caspase-3 are not valuable in discriminating the number of planned operations, even though there is a significant decrease in caspase-3 "within" survivors. The discriminative value of caspase-3 for closure should be evaluated in studies in which caspase-3 is monitored for a longer duration in a large number of patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2011

Surgical management with or without a nasogastric tube in esophageal repairs.

Int J Pediatr Otorhinolaryngol 2012 Jan 8;76(1):104-6. Epub 2011 Nov 8.

Department of Pediatric Surgery, Meram Medical School of Selcuk University, Konya, Turkey.

Objective: The aim of the study was to compare surgical management with or without a nasogastric tube (NGT) to prevent anastomotic stricture that occurred following esophageal repairs (ERs).

Methods: Twelve New Zealand rabbits were divided equally into 2m: with a NGT (experimental group) and without a NGT (control group). A 1-cm-length of the cervical esophagus was resected through a cervical incision and then anastomosis was performed using the NGT and keeping it in place for 6 days in the experimental group. The same procedures were performed in the control group. Both groups were fed parenterally for 6 days and orally after esophagography on postoperative day 7 as long as there was no esophageal leakage. The rabbits were sacrificed to evaluate diameter of the esophageal lumen (DOTEL), bursting pressure (BP), tissue hydroxyproline (HP) and wound healing scores (WHSs) in the anastomosis lines 8 weeks later.

Results: In the experimental group, DOTEL, BP, and HP were significantly lower than they were in the control group. WHSs in the experimental group were not higher than they were in the control group.

Conclusions: Surgical management without a NGT is more effective than management with a NGT in ERs as shown by increased DOTEL, BP, and HP levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijporl.2011.10.012DOI Listing
January 2012

Effects of ovariectomy and ascorbic acid supplement on oxidative stress parameters and bone mineral density in rats.

Libyan J Med 2011 3;6. Epub 2011 Oct 3.

Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey.

Objectives: The aim of this study is to investigate the effects of ovariectomy on bone mineral density (BMD) and oxidative state in rats, and the alterations in these effects that vitamin C supplementation may produce.

Materials And Methods: TWENTY FEMALE WISTAR ALBINO RATS WERE RANDOMLY DIVIDED INTO THREE GROUPS: control (C, n=6); ovariectomy (O, n=7); and ovariectomy+vitamin C supplement (OV, n=7). Oxidative stress (OS) was assessed 100 days postovariectomy by measuring the activity of several enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase, as well as the concentrations of malondialdehyde (MDA), nitric oxide (NO), and total sulfhydryl groups in plasma and bone homogenates.

Results: A significant decrease in BMD was observed in O group compared with C group (p=0.015), and a significant increase was observed in OV compared with O group (p=0.003). When groups were compared with respect to parameters of OS, MDA and NO levels in bone tissue were significantly higher in O than in C (p=0.032, p=0.022) and were significantly lower in OV than in O (p=0.025, p=0.018). SOD activity was significantly higher in O than in C (p=0.032). In plasma, MDA activity was significantly higher in O than in C (p=0.022) and NO level was significantly higher in O than in C and OV (p=0.017, p=0.018).

Conclusions: Our results suggest that ovariectomy may produce osteoporosis and OS in females, and vitamin C supplementation may provide alterations regarding improvement in OS and BMD values. We assume that studies including more subjects are needed to make a decisive conclusion about OS-BMD relation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3402/ljm.v6i0.5965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188298PMC
November 2011

The effects of early and late feeding on healing of esophageal anastomoses: an experimental study.

Int J Pediatr Otorhinolaryngol 2011 Oct 11;75(10):1289-91. Epub 2011 Aug 11.

Department of Pediatric Surgery, Meram Medical School of Selcuk University, Konya, Turkey.

Objective: To investigate the effects of postoperative late and early feeding on the healing of experimental esophageal anastomosis (EA).

Methods: Twelve New Zealand rabbits were divided equally into 2 groups: late feeding (LF) and early feeding (EF). A 1-cm-length of the cervical esophagus was resected through a cervical incision, and then anastomosis was performed. The LF group was fed parenterally for 6 days and orally (per oral route) after esophagography on postoperative day 7 as long as there was no esophageal leakage. The EF group was fed orally (per oral route) 24h after esophageal repairs (ERs), and esophagography was carried out on postoperative day 7 to check the ERs. The rabbits were sacrificed to evaluate bursting pressure (BP), diameter of the esophageal lumen (DOTEL), tissue hydroxyproline (HP), and wound healing scores (WHSs) in the anastomosis lines 8 weeks later.

Results: In the LF group, BP and DOTEL were significantly lower than they were in the EF group, and HP was significantly higher than it was in the EF group. WHSs in the LF group were not higher than they were in the EFgroup.

Conclusions: Early feeding is superior after EA as shown by increased DOTEL and BP levels, but causes decreased HP levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijporl.2011.07.013DOI Listing
October 2011

Effect of phototherapy on gastrointestinal smooth muscle activity and oxidative stress.

Pediatr Surg Int 2011 Nov 1;27(11):1197-202. Epub 2011 Jun 1.

Department of Pediatric Surgery, Medical Faculty, Kirikkale University, Kirikkale, Turkey.

Aim: To evaluate the effect of phototherapy on gastrointestinal smooth muscle activity and oxidative stress.

Methods: Wistar albino rats (n = 18, in the first 7 days of life) weighing 7 ± 2 g with both sexes were included in the study. The animals were randomized into three groups. In control group (CG), median laparotomy was performed to obtain 1 cm of jejunum, terminal ileum and colonic segments. In the phototherapy group (PTG), led phototherapy with a wave density of 40 μw/cm(2)/nm were used (Bilitron 3006, Fanem, Brasil). The efficacy surface of phototherapy was 30-40 cm and the exposure distance was 30 cm. The duration of phototherapy was 24 h. Sham group (SG) received white light with the same wave density and exposure distance. The oxidative stress markers and contraction responses were investigated from intestinal segments obtained from experiments.

Results: The jejunum segments showed significantly lowered contraction response to carbachol in SG when compared to CG and PTG (p < 0.05). Decreased contractile response to KCl was detected in both SG and PTG in terminal ileum segments. MDA levels showed no difference between groups (p > 0.05). Total sulfhydryl (T-SH) levels were found significantly increased in PTG when compared to CG and SG (p < 0.05). When NO levels were evaluated, NO levels were found decreased in PTG and SG with respect to CG (p < 0.05).

Conclusion: PT may cause various alterations in oxidant/antioxidant system in intestinal segments. Unlike to clinical findings, decreased contractile responses were detected in rat gastrointestinal smooth muscles after PT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00383-011-2934-0DOI Listing
November 2011

Comparison of hematological and biochemical parameters in sheep naturally and persistently infected with a border disease virus.

Trop Anim Health Prod 2011 Mar 21;43(3):553-6. Epub 2010 Nov 21.

Department of Internal Medicine, School of Veterinary Medicine, Kirikkale University, 71451, Kirikkale, Turkey.

In this study, we investigated the changes occurring in the activities of determining the biochemical and hematological parameters in persistently infected sheep with border disease virus (BDV) and control sheep. While cholesterol, aspartate aminotransferase, lactate dehydrogenase, high-density lipoprotein, and glucose parameters were found to be statistically different between control and BDV positive groups (p<0.01), total protein, alkaline phosphotase, creatine kinase, amylase, glucose, and high-density lipoprotein were found to be statistically different between control and persistently infected group (p<0.01). Interestingly, all groups were shown only mean corpuscular volume parameter was different (p<0.01). It was found that cholesterol, aspartate aminotransferase, amylase, high-density lipoprotein, and low-density lipoprotein parameters were different between PI and infected sheep (p<0.01). It was speculated that BDV might effect also the expression of low-density lipoprotein receptor and determination of the changes in BD and its clinical importance might contribute to the veterinarians and scientists studying in this area.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11250-010-9754-4DOI Listing
March 2011

Cotinine levels in serum and bronchoalveolar lavage fluid.

Southeast Asian J Trop Med Public Health 2010 Sep;41(5):1252-7

Department of Chest Diseases, Ankara University School of Medicine, Ankara, Turkey.

Cotinine is a major metabolite of nicotine. This study was planned to investigate the relationship between bronchoalveolar lavage (BAL) fluid cotinine levels and serum cotinine levels in smokers and nonsmokers with various pulmonary diseases and to investigate whether these levels are affected by passive smoking. Serum and BAL fluid cotinine levels were measured in 27 patients. BAL cotinine levels were measured using a sensitive ELISA kit produced to measure cotinine in saliva. Plates were read by microuant (BioTek, USA) micro plate reader. All patient serum cotinine levels were detectable except for one nonsmoker patient. However, BAL fluid cotinine levels were measurable in only 6 patients (two of them were nonsmokers). A significant positive correlation was seen between serum and BAL fluid cotinine levels (r = 0.726; p = 0.000). Serum cotinine levels were significantly higher in present smokers than non-smokers (21.0 +/- 16.01; 5.35 +/- 7.65; p = 0.004). However, there were no significant differences in BAL fluid cotinine levels between smokers and nonsmokers. Passive smoking can increase nicotine metabolites in serum and other body fluids, including BAL fluid. Since BAL fluid and serum cotinine levels were well correlated, there is no need to use invasive procedures, such as bronchoscopy and expensive, time consuming BAL fluid analyses. Serum cotinine levels can give a rough idea of smoking status. BAL fluid cotinine meaurements should be done for only scientific reasons.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2010

Celiac disease prevalence in patients with iron deficiency anemia of obscure origin.

Turk J Gastroenterol 2009 Dec;20(4):266-70

Department of Internal Medicine, Kirikkale University, School of Medicine, Kirikkale.

Background/aims: Anemia, especially due to iron deficiency, is a frequent feature in celiac disease. In this study, we aimed to define the prevalence of celiac disease in Turkish patients with iron deficiency anemia of obscure origin.

Methods: One thousand four hundred and eighty-six consecutive patients with iron deficiency anemia were evaluated for etiology. Of those, 77 patients were found to have iron deficiency anemia of obscure origin. Sera from 77 patients with iron deficiency anemia of obscure origin and 119 healthy controls were tested for IgA and IgG tissue transglutaminase (tTG) antibodies by ELISA. Endoscopic mucosal biopsies were taken from the second part of the duodenum in these patients. Histopathologic examination results of patients were stratified according to Marsh classification.

Results: IgA and IgG class anti-tTG antibodies were found positive in 6 (7.8%) and 3 (3.9%) patients with iron deficiency anemia of obscure origin, respectively. Three patients had only IgA anti-tTG and 3 had both IgA and IgG anti-tTG antibodies. In the control group, 1 subject was positive for both IgA and IgG anti-tTG antibodies (0.7%). Six patients (7.8%) and 1 control subject (0.8%) had histopathologic findings of celiac disease (p=0.02).

Conclusions: Patients with iron deficiency anemia of obscure origin had increased prevalence of celiac disease. Our study results suggest that serological screening may be recommended for early detection of celiac disease in patients with iron deficiency anemia of obscure origin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4318/tjg.2009.0024DOI Listing
December 2009

Periodontal health in children exposed to passive smoking.

J Clin Periodontol 2010 Feb 21;37(2):160-4. Epub 2009 Dec 21.

Department of Periodontology, Faculty of Dentistry, Kirikkale University, Kirikkale, Turkey.

Aim: To determine (1) the cotinine levels of saliva, urine and gingival crevicular fluid (GCF) of children in families with and without smoking members and (2) a possible association between the periodontal health of the children and exposure to passive smoking.

Material And Methods: The study population comprised of 109 children in the age range 6-12 years. Children were classified as exposed to passive tobacco smoking (PTS-exposed, n=51) and as unexposed controls (PTS-unexposed, n=58). Plaque index, gingival index, bleeding on probing, probing depth and clinical attachment level (CAL) were recorded. GCF, saliva and urine samples were also collected. The levels of cotinine in these fluids were determined by enzyme-linked immunosorbent assay.

Results: The mean salivary cotinine concentration was significantly increased in PTS-exposed children compared with PTS-unexposed children (p<0.05). Further, in a dose-dependent way, the mean salivary concentration was significantly higher in children whose father or mother was a smoker (p<0.05) as compared, respectively, with children whose fathers and mothers were non-smokers. The mean CAL was significantly less in PTS-exposed children compared with non-PTS-exposed children (0.09 mm; p<0.05) and also in children whose father was a smoker (p<0.05), but not in children whose mother was a smoker as compared with non-smoker fathers and mothers, respectively. The GCF cotinine levels were below the detection limits with the assay method that was used.

Conclusions: We have observed that children who are exposed to passive smoking have elevated cotinine levels in their saliva concomitant with a lowered CAL.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1600-051X.2009.01510.xDOI Listing
February 2010

In vitro antibacterial activity of some systemic and topical antihistaminic preparations.

Clin Invest Med 2009 Dec 1;32(6):E232. Epub 2009 Dec 1.

Department of Microbiology and Clinical Microbiology, Kirikkale University, 71100 Kirikkale, Turkey.

Purpose: In vitro antibacterial activity of topical and systemic antihistaminic preparations containing different active substrates against the standard strains of two bacteria was evaluated.

Methods: Four topical and 3 systemic preparations containing pheniramine maleate, chlorophenoxamine hydrochloride, and diphenhydramine hydrochloride were studied. The antibacterial activities of these preparations against strains of S. aureus (American Type Culture Collection, ATCC 29213) and S. epidermidis (ATCC 25212) were tested using the disc diffusion method. In addition, the Minimal Innhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of parenteral preparations for these two bacteria were determined.

Results: Pheniramine maleate-topical and pheniramine maleate-systemic had no activity against bacteria, but the others showed various rates of activity. Chlorophenoxamine hydrochloride-topical and chlorophenoxamine hydrochloride-systemic were the most effective (P < 0.05). Despite the same active substrate content, diphenhydramine hydrochloride-topical-1 and diphenhydramine hydrochloride-topical-2 yielded different results when they were compared with each other or with the other preparations. Diphenhydramine hydrochloride-topical-2 had a relatively higher rate of activity than diphenhydramine hydrochloride-topical-1. Inhibition zone diameters were 16.9+/-1.5 mm 12.3+/-0.5 mm for S .aureus, 17.4+/-1.0 mm 0 mm for S .epidermidis respectively (P < 0.05). MIC values of parenteral preparations were equal to or above 125 ?g/ml.

Conclusion: MIC values of parenteral preparations were higher than their blood levels in clinical use. Thus, effects of parenteral preparations may not have been reflected in routine clinical practice. However, topical forms have antibacterial activity due to additive substrates and the use of high concentration levels at the site of application. Therefore, in selection of topical forms for appropriate cases, these effects should also be taken into consideration. The antibacterial activity of topical antihistaminic preparations may be useful in certain dermatological pathology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.25011/cim.v32i6.10657DOI Listing
December 2009

The healing effects of tissue glues and healing agent locally applied on esophageal anastomoses.

Int J Pediatr Otorhinolaryngol 2010 Jan 6;74(1):43-6. Epub 2009 Nov 6.

Department of Pediatric Surgery, Meram Medical School of Selcuk University, Konya, Turkey.

Objective: This study aimed to investigate the effects of cyanoacrylate (C), fibrin glue (FG), and natrium hyaluronate (NH) on the healing of esophageal anastomosis (EA).

Methods: Twenty-four rabbits were divided equally into 4 groups: primary anastomosis (PA), C, FG, and NH. A 1-cm-length of the cervical esophagus was resected through a cervical incision and then anastomosis was performed. C, FG, and NH were instilled into anastomosis lines in the respective groups. The animals were fed orally on postoperative day 7 on the condition that there was no esophageal leakage. The rabbits were sacrificed 8 weeks later to evaluate bursting pressure (BP), tissue hydroxyproline (HP) levels and wound healing scores (WHSs) in the anastomosis lines.

Results: BP was significantly higher in the C group than in the PA, FG, and NH groups, and HP was significantly lower than in the other groups. WHSs in the PA and NH groups were lower than in the C and FG groups.

Conclusions: C and NH appear to be beneficial in EA healing with respect to increased BP and decreased HP when they are used simultaneously with PA prophylactically to prevent esophageal leakages and stricture.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijporl.2009.10.006DOI Listing
January 2010

The relationship between serum adiponectin level and anthropometry, bone mass, osteoporotic fracture risk in postmenopausal women.

Eklem Hastalik Cerrahisi 2009 ;20(2):78-84

Department of 5th Orthopedics and Traumatology, Ankara Numune Education and Research Hospital, Ankara, Turkey.

Objectives: The aim of the present study was to evaluate the possible correlation between bone mass and serum adiponectin levels, and the correlation between adiponectin levels and osteoporotic fracture risk in a prospective clinical trial.

Patients And Methods: Postmenopausal non-diabetic 105 women (mean age 63.4+/-8.1; range 52 to 64 years) with hip fracture were evaluated. Of these 105 patients, 46 had trochanteric fractures, 24 had subtrochanteric fractures and 35 had femoral neck fractures. Anthropometric measurements were performed. Serum adiponectin level was measured by means of ELISA. Total bone mineral density and bone mineral content of lumbar spine and proximal femur were measured by dual-energy X-ray absorptiometry (DEXA).

Results: Lumbar bone mineral density and proximal femoral bone mineral density were not correlated with serum adiponectin levels. Serum adiponectin level was not found to have any significant effect on bone mass. Serum adiponectin levels were not significantly different between the patients with osteoporotic fractures and those with non-osteoporotic fractures.

Conclusion: Our study showed that serum adiponectin level is not associated with bone mass and osteoporotic fracture risk. Investigation of local adiponectin levels in bony tissue is needed to clarify the possible relation between adiponectin and bone mass, and risk of fractures associated with osteoporosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
February 2010

The effect of hypothermia on splanchnic flows and lung in a two-hit hemorrhagic shock model.

J Surg Res 2010 Jan;158(1):121-6

Department of Emergency Medicine, Kirikkale University Medical School, Suleyman Demirel Research and Training Hospital, Kirikkale, Turkey.

Background: To evaluate the effect of hypothermia on bacterial translocation, splanchnic vascular flow, lung tissue weight, and levels of malondialdehyde (MDA) and nitric oxide (NO) in a two-hit model of hemorrhagic shock.

Methods: Thirty rats were randomly allocated into three groups of 10 rats each. In the control group (group C), rats were treated without hemorrhage, and normothermia (37 degrees C) was maintained. In the mild hypothermia group (group MH), rats were subjected to volume-controlled hemorrhage (2 mL/100g) and a rectal temperature of 34 degrees C was maintained. In the normothermic group (group NT), rats were treated as in group MH, except for hypothermia. Seventy-two hours after hemorrhagic shock (first insult), Pseudomonas aeuruginosa was administered intratracheally as a second insult. Finally, mesenteric vascular flow patterns were recorded. Bacterial translocation was studied from tissue samples of spleen, liver, and mesenteric lymph nodes. Blood samples were obtained to evaluate the possible presence of bacteria in the bloodstream. Lung tissue weight ratio, MDA, and NO levels in lung tissue were assessed.

Results: Renal, mesenteric, and portal venous flow rates were found to be lower in groups MH and NT in comparison with group C. Blood flow profiles were lower in group NT than in group MH (P<0.05). Bacterial translocation was not observed in group C, and it was detected more often in group NT than in group MH. Lung weight ratio was found to be higher in group NT compared with groups MH and C. Although it did not reach the level of statistical significance, MDA level in the control group was lower than that in the NT group (P=0.085).

Conclusion: Hypothermia corrected mesenteric blood flow and decreased the occurrence of bacterial translocation in the two-hit model of hemorrhagic shock and tracheal inoculaton of P. aeruginosa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jss.2008.08.029DOI Listing
January 2010

Effect of carnitine on cutaneous wound healing in immunosuppressed rats.

J Surg Res 2009 Aug 11;155(2):301-5. Epub 2008 Jul 11.

Department of General Surgery, Kirikkale University Medical School, Suleyman Demirel Research and Training Hospital, Kirikkale, Turkey.

Background: The wound is ischemic in nature. Chronic steroid administration impairs wound healing by changing enzymes in the glycolytic pathway. Carnitine supplementation may help to restore the energy deficiency caused by chronic steroid administration in the wound. The aim of this study was to evaluate the effect of carnitine on impaired wound healing.

Methods: The study was conducted in three groups. Surgical intervention was a 4 cm long midline skin incision at the back. In Group A, eight rats received methylprednisolone for 7 d prior to surgical intervention, and it was continued until the end of the experiment. In Group B, 12 rats received methylprednisolone for 7 d prior to surgical intervention. After surgery, methylprednisolone injection was continued and carnitine was supplemented until the end of the experiment. In Group C, eight rats received no medication. The wound of half of the animals in each group was harvested on the seventh day after surgical intervention and the remaining on the 14th d. Tensile strength and hydroxyproline content were measured in all groups.

Results: There was no significant difference in parameters in any of the groups on day seven. On day 14, all parameters were statistically different between methylprednisolone and control groups (P < 0.05). Values for tensile strength were higher in the methylprednisolone/carnitine group compared with methylprednisolone group (P < 0.05). Carnitine administration had also increased hydroxyproline levels in the methylprednisolone/carnitine group compared with the control group (P < 0.05).

Conclusions: Carnitine is shown to increase tensile strength of the wound when supplemented to immunosuppressed rats in which wound healing is impaired by methylprednisolone.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jss.2008.06.010DOI Listing
August 2009

The effect of cyanoacrylate in esophagocutaneous leakages occurring after esophageal anastomosis.

Int J Pediatr Otorhinolaryngol 2009 Jul 6;73(7):1053-5. Epub 2009 May 6.

Department of Pediatric Surgery, Meram Medical School of Selcuk University, Konya, Turkey.

Objective: Esophageal leakage (EL) continues to be a challenging pediatric surgical problem. The aim of this study was to investigate the effect of cyanoacrylate (Cy) in EL followed esophageal repair (ER).

Methods: Twelve rabbits were divided into control (C) and leakage (L) groups. A 1cm-length transverse esophageal incision at the level of the cervical region was made. In both groups, feeding was started orally 24h after the surgery for leakage creation. On postoperative day 7, primary repair was carried out in the C group and Cy instillation was performed in the L group. Esophagographic analysis was carried out on postoperative day 9 and the animals were fed orally on the same day on the condition that there was no esophageal leakage. The rabbits were sacrificed to measure diameters of the OR line, bursting pressure (BP), and hydroxyproline (HP) levels in the repaired cervical esophageal segment (RCES) 2 months later.

Results: The values of BP and HP in the C group were significantly higher than those in the L group. The diameters of the OR line in the L group were significantly greater compared to those in the C group.

Conclusions: Cy glue instillation seems to be the ideal treatment for esophageal anastomosis leakages as shown by increased diameters of the OR line and decreased HP levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijporl.2009.04.013DOI Listing
July 2009

Evaluation of systemic markers related to anemia of chronic disease in the peripheral blood of smokers and non-smokers with chronic periodontitis.

Eur J Dent 2008 Apr;2(2):102-9

Kirikkale University, Faculty of Dentistry, Department of Periodontology, Kirikkale, Turkey.

Objectives: The aim of this study was to investigate the effect of cigarette smoking on clinical parameters and signs anemia of chronic disease in chronic periodontitis patients.

Methods: The study base consisted of 88 patients with chronic periodontitis including 45 volunteer current smokers with age range of 30-69 (45.5+/-8.5) and 43 volunteer non-smokers with age range of 32-61 years (45.8+/-7.9). The clinical parameters including plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD), clinical attachment loss (CAL) were recorded and several red blood cell parameters were determined from peripheral blood samples.

Results: In smokers, PI, PD and CAL were significantly higher than non-smokers (P<.05). The number of erythrocytes and the levels of hemoglobin, hematocrit and iron were lower in smokers compared to non-smokers (P<.05).

Conclusions: In the present study, it is concluded that cigarette smoking may be effective on the signs of anemia of chronic disease in patients with chronic periodontitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633164PMC
April 2008

The effect of indwelling catheter on OH-proline in the urethral wound: an experimental study.

J Pediatr Urol 2006 Jun 18;2(3):182-4. Epub 2005 Aug 18.

Kirikkale University School of Medicine, Department of Pediatric Surgery, Turkey.

Objective: To investigate the effect of catheterization on urethral wound healing by measuring OH-proline.

Methods: Eighteen male New Zealand albino rabbits were divided equally into three groups. In the control group, urethral tissue samples were taken from the ventral penile surface. In group II, the ventral penile surface was incised and primarily repaired by 6/0 polydioxanone. In group III, the incised urethra was repaired by 6/0 polydioxanone and a urethral catheter was inserted. Tissue samples were taken on the 20th postoperative day in groups II and III. Tissue OH-proline levels were measured by the method of Bergman and Loxley. The Kruskal-Wallis statistical test was used for evaluation.

Results: OH-proline values as microg/mg dry tissue were 16.43+/-2.68 in the control group, 14.47+/-1.82 in group II and 14.63+/-0.63 in group III. There was no significant difference between the three groups (P>0.05).

Conclusion: Use of a urethral catheter does not appear to have a direct effect on collagen synthesis in wound healing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpurol.2005.07.003DOI Listing
June 2006

Evaluation of serum resistin levels in patients with ocular and non-ocular Behçet's disease.

Can J Ophthalmol 2008 Aug;43(4):473-5

Department of Ophthalmology, Ankara University Faculty of Medicine, Ankara, Turkey.

Background: Resistin, a recently identified adipocytokine, has been found to play an important role in inflammation and the processes of inflammation-related diseases. Serum resistin levels in patients with Behçet's disease (BD) have not yet been investigated. We aimed to evaluate the relation between resistin and interleukin-6 (IL-6) in Behçet patients with or without ocular involvement and in normal controls.

Methods: Twenty-two patients with BD and 19 healthy control subjects were included in this study. While 14 patients had posterior segment involvement of the eye, the other 8 did not have ocular disease. Serum resistin and interleukin-6 (IL-6), levels were measured in all samples. Data from all groups were tested for statistical significance.

Results: The mean resistin and IL-6 concentrations were significantly higher in patients with BD than the control subjects (p = 0.011 and p = 0.0001, respectively). There was a significant difference in resistin and IL-6 levels between the patients with non-ocular BD and controls (p = 0.013 and p = 0.0001, respectively), as well as resistin and IL-6 levels between the ocular BD group and the control group (p = 0.05 and p = 0.0001, respectively). However, there was no significant difference between patients with ocular versus non-ocular BD.

Interpretation: Resistin levels were found to be raised in Behçet patients with or without ocular involvement compared with the control subjects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3129/i08-081DOI Listing
August 2008

In vitro antibacterial effects of topical local anesthetics.

J Dermatolog Treat 2008 ;19(6):351-3

Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey.

Background: The antibacterial activities of local anesthetics are recognized.

Objective: To investigate in vitro the activity of topical local anesthetic ointments at clinical doses.

Methods: The activity of two different local anesthetic ointments including lidocaine 5% and lidocaine/prilocaine 2.5% was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes and Enterococcus faecalis by the disc-diffusion method. Sterile discs containing topical local anesthetic drugs were prepared taking into account the doses of ointments used in clinical practice. The validity of the methodology was confirmed using topical antibacterial mupirocin. The inhibition zones of the discs were measured.

Results: Mupirocin inhibited all the bacteria. Both local anesthetic ointments were found to be most effective on E. coli, whereas they had no effects on P. aeruginosa. Lidocaine 5% revealed antibacterial activity against S. aureus, S. epidermidis, E. coli, S. pyogenes and E. faecalis, but lidocaine/prilocaine 2.5% showed no activity on E. faecalis and inhibited S. pyogenes only at double doses. It was also observed that the antibacterial activity was in a dose-dependent manner.

Conclusion: In the light of these findings, it might be concluded that topical local anesthetic ointments in routine settings may have a preventive role against some bacteria.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09546630802050498DOI Listing
February 2009

In vitro investigation of the antibacterial effect of ketamine.

Ups J Med Sci 2008 ;113(1):39-46

Department of Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey.

Background: Antibacterial activity of local anesthetics especially lidocaine has been shown previously. In this study, the antibacterial effect of ketamine, a general anesthetic agent was investigated.

Methods: The antibacterial effect of ketamine was studied using six different strains of bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Entecoccus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli) with disc diffusion method. Ciprofloxacine discs (CIP, oxoid) were used as a control to verify the methodology. Minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) of ketamine for these bacteria were also determined.

Results: No inhibition was evident in discs containing 62.5 microg of ketamine. Ketamine 125 microg showed activity on all the bacteria tested with the exception of E. coli. The inhibition rates of Ketamine were more prominent at the doses of 250 microg and 500 microg similar to the inhibition rate of CIP. Whereas MIC and MBC values of ketamine for S. aureus and S. pyogenes were 500 microg mL(-1), MIC and MBC values for P. aeruginosa were above 2000 microg mL(-1). For other bacteria, these values ranged between these levels.

Conclusions: Ketamine with higher doses showed antibacterial activity. We thought that it will be proper to use ketamine hesitantly in experimental animal studies like sepsis and translocation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3109/2000-1967-211DOI Listing
August 2008

The effects of the non-volatile anaesthetic agents, propofol and thiopental, on erythrocyte sedimentation rate.

Ups J Med Sci 2007 ;112(3):335-7

Department of Biochemistry, Kirikkale University School of Medicine, Kirikkale, Turkey.

Objectives: The reduction of erythrocyte sedimentation rate (ESR) induced by general anaesthesia was demonstrated in our previous study. The purpose of the present study was to investigate whether the type of induction agent (propofol or thiopental) used for general anaesthesia had any effects on ESR.

Methods: Sixty-four patients (ASA Physical Status Classification, I-II) scheduled for elective surgery under general anaesthesia were randomly assigned into two groups. In Group I, propofol and in Group II, thiopental were used as induction agents. Two blood samples were obtained before induction and 10 minutes after endotracheal intubation for ESR measurements.

Results: The ESR values of the second samples from both groups were significantly lower than the values of the first samples, but there were no statistically significant differences in ESR values between the values of the two groups.

Conclusion: The results showed that general anaesthesia decreased ESR values regardless of the type of agents being used for induction of anaesthesia. The reason might be related to other drugs used in both groups, or to a common effector mechanism of the two induction agents. The underlying mechanism needs to be investigated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3109/2000-1967-205DOI Listing
August 2008

Impact of adiponectin on left ventricular mass index in non-complicated obese subjects.

Endocr J 2008 Jul 9;55(3):523-8. Epub 2008 May 9.

Kirikkale University Faculty of Medicine Department of Cardiology, Kirikkale, Turkey.

To evaluate the relationship between the adiponectin levels and left ventricular mass index (LVMI) in uncomplicated obese subjects. Fifty-nine subjects were assigned to the obese (BMI> or =30 kg/m(2)) and 58 to the lean (BMI<30 kg/m (2) ) group. Plasma glucose, insulin, serum total cholesterol and high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, triglycerides and adiponectin were measured. Insulin resistance was determined by the Homeostasis Assessment Model (HOMA-IR). The left ventricular functions of all subjects were determined by 2D and pulse wave Doppler echocardiography. LVMI was calculated as left ventricular mass (LVM) normalized for height in m (2.7) . The obese group displayed significantly higher LVMI and late mitral inflow velocity. Thirty-three obese subjects met the criteria for left ventricular hypertrophy (LVH) and had lower serum adiponectin levels compared with obese subjects without LVH and lean subjects (p<0.05). Adiponectin was negatively correlated with LVMI (R: -0.277, p: 0.002). Furthermore, during the partial correlation analysis where HOMA-IR was controlled, the negative correlation between adiponectin and LVMI progressed (r: -0.283, p: 0.002). The linear regression analysis showed an independent relationship between LVMI and adiponectin. (beta: -0.214, p: 0.01) Obesity is associated with LVH. This study showed direct influence of adiponectin on LVMI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1507/endocrj.k07e-098DOI Listing
July 2008

Evaluation of adrenomedullin and endothelin-1: are they factors in the adaptation of maternal vascular system in normotensive pregnancy.

Adv Ther 2008 Apr;25(4):367-74

Department of Obstetrics and Gynaecology, Faculty of Medicine, Gazi University, Bilkent-3 C: 4 No: 7, Bilkent, Ankara, 06800, Turkey.

Introduction: The aim of our study was to analyse the role of adrenomedullin (AM) and endothelin-1 (ET-1) in the adaptation of the maternal vascular system in normotensive pregnancy.

Methods: Twenty-eight pregnant women, who were normotensive throughout the duration of their pregnancy, were recruited into the study. Plasma levels of AM and ET-1 at each trimester were measured and the AM/ET-1 ratio was calculated.

Results: Our experiment showed a significant decrease in plasma concentrations of AM in the first trimester for the study group (n=28) compared with the non-pregnant control group (n=16). There was also a significant decrease in plasma concentrations of ET-1 in all three trimesters (P<0.05) and a significant increase in the AM/ET-1 ratio in all three trimesters (P<0.05) for the study group compared with the control group.

Conclusion: An alteration in vascular equilibrium between AM and ET-1, favouring AM, may be a reason why the physiological adaptation of the maternal vascular system to pregnancy occurs during normotensive pregnancy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12325-008-0041-yDOI Listing
April 2008
-->