Publications by authors named "Omid Vakili"

9 Publications

  • Page 1 of 1

Gastrointestinal cancer drug resistance: the role of exosomal miRNAs.

Mol Biol Rep 2021 Nov 30. Epub 2021 Nov 30.

Behbahan Faculty of Medical Sciences, Behbahan, Iran.

Resistance of gastrointestinal (GI) cancer cells to therapeutic agents are one of the major problems in treating this type of cancer. Although the exact mechanism of drug resistance has not yet been fully elucidated, various factors have been identified as contributing factors involved in this process. Several studies have revealed the role of exosomes, especially exosomal microRNAs (miRNAs), in GI tumorigenesis, invasion, angiogenesis, and drug resistance. Exosomes, a type of small extracellular vesicles (EVs), are originated from endosomes and are released into the extracellular environment and body fluids by different cell types. Exosomes mediate cell-cell communication by transferring different cargos, including miRNAs, between parent and recipient cells. Therefore, identifying these exosomal miRNAs and their functions in GI cancers might provide new clues to further explore the secret of this process and thus help in drug-resistance management. This review article will discuss the roles of exosomal miRNAs and their mechanisms of action in drug resistance of different types of GI cancer cells (e.g., stomach, esophagus, liver, pancreas, and colon) to therapeutic agents.
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http://dx.doi.org/10.1007/s11033-021-07007-3DOI Listing
November 2021

microRNAs in female infertility: An overview.

Cell Biochem Funct 2021 Dec 27;39(8):955-969. Epub 2021 Oct 27.

Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Infertility impacts a considerable number of women worldwide, and it affects different aspects of family life and society. Although female infertility is known as a multifactorial disorder, there are strong genetic and epigenetic bases. Studies revealed that miRNAs play critical roles in initiation and development of female infertility related disorders. Early diagnosis and control of these diseases is an essential key for improving disease prognosis and reducing the possibility of infertility and other side effects. Investigating the possible use of miRNAs as biomarkers and therapeutic options is valuable, and it merits attention. Thus, in this article, we reviewed research associated with female diseases and highlighted microRNAs that are related to the polycystic ovary syndrome (up to 30 miRNAs), premature ovarian failure (10 miRNAs), endometriosis (up to 15 miRNAs), uterine fibroids (up to 15 miRNAs), endometrial polyp (3 miRNAs), and pelvic inflammatory (6 miRNAs), which are involved in one or more ovarian or uterine disease-causing processes.
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http://dx.doi.org/10.1002/cbf.3671DOI Listing
December 2021

Circular RNAs; powerful microRNA sponges to overcome diabetic nephropathy.

Pathol Res Pract 2021 Nov 15;227:153618. Epub 2021 Sep 15.

Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran; Department of Clinical Biochemistry, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Electronic address:

Diabetic nephropathy (DN), also known as diabetic kidney disease (DKD), is a drastic renal complication of type 1 and type 2 diabetes mellitus (DM). Poorly controlled DM over the years, may disrupt kidneys' blood vessels, leading to the hypertension (HTN) and DN onset. During DN, kidneys' waste filtering ability becomes disturbed. Being on a healthy lifestyle and controlling both DM and HTN are now the best proceedings to prevent or at least delay DN occurrence. Unfortunately, about one-fourth of diabetic individuals eventually experience the corresponding renal failure, and thus it is critical to discover effective diagnostic biomarkers and therapeutic strategies to combat DN. In the past few years, circular RNAs (circRNAs), as covalently closed endogenous non-coding RNAs (ncRNAs), are believed to affect DN pathogenesis in a positive manner. CircRNAs are able to impact different cellular processes and signaling pathways by targeting biological molecules or various molecular mechanisms. Still, as a key regulatory axis, circRNAs can select miRNAs as their molecular targets, in which they are considered as miRNA sponges. In this way, circRNA-induced suppression of particular miRNAs may prevent from DN progression or promotes the DN elimination. Since the expression of circRNAs has also been reported to be increased in DN-associated cells and tissues, they can be employed as either diagnostic biomarkers or therapeutic targets.
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http://dx.doi.org/10.1016/j.prp.2021.153618DOI Listing
November 2021

Bispecific antibodies in colorectal cancer therapy: recent insights and emerging concepts.

Immunotherapy 2021 11 13;13(16):1355-1367. Epub 2021 Oct 13.

Department of Microbiology & Immunology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.

Colorectal cancer (CRC) is identified as a life-threatening malignancy. Despite several efforts and proceedings available for CRC therapy, it is still a health concern. Among a vast array of novel therapeutic procedures, employing bispecific antibodies (BsAbs) is currently considered to be a promising approach for cancer therapy. BsAbs, as a large family of molecules designed to realize two distinct epitopes or antigens, can be beneficial microgadgets to target the tumor-associated antigen pairs. On the other hand, applying the immune system's capabilities to attack malignant cells has been proven as a tremendous development in cancer therapeutic projects. The current study has attempted to overview some of the approved BsAbs in CRC therapy and those under clinical trials. For this purpose, reputable scientific search engines and databases, such as PubMed, ScienceDirect, Google Scholar, Scopus, etc., were explored using the keywords 'bispecific antibodies', 'colorectal cancer', 'immunotherapy' and 'tumor markers'.
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http://dx.doi.org/10.2217/imt-2021-0107DOI Listing
November 2021

Finding appropriate signal peptides for secretory production of recombinant glucarpidase: an in silico method.

Recent Pat Biotechnol 2021 Sep 20. Epub 2021 Sep 20.

Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz. Iran.

Aims: Bioinformatics analysis of suitable signal peptide for recombinant glucarpidase.

Background: Methotrexate (MTX) is a general chemotherapeutic agent utilized to treat a variety of malignancies., woefully, its high doses can cause nephrotoxicity and subsequent defect in the process of MTX excretion. The recombinant form of glucarpidase, is produced by engineered E. coli and is a confirmed choice to overcoming this problem.

Objective: In the present study, in silico analyses were performed to select suitable SPs for the secretion of recombinant glucarpidase in E. coli.

Methods: The signal peptide website and UniProt database were employed to collect the SPs and protein sequences. In the next step, SignalP-5.0 helped us to predict the SPs and the position of cleavage sites. Moreover, physicochemical properties and solubility were evaluated using ProtParam and Protein-sol online software, and finally, ProtCompB was used to predict the final sub-cellular localization.

Results: Luckily, all SPs could form soluble fusion proteins. At last, it was found that PPB and TIBA could translocate the glucarpidase into the extracellular compartment.

Conclusion: This study showed that there are only 2 applicable SPs for the extracellular translocation of glucarpidase. Although the findings were remarkable with high degrees of accuracy and precision based on the utilization of bioinformatics analyses, additional experimental assessments are required to confirm and validate it. Recent patents revealed several inventions related to the clinical aspects of vaccine peptide against human disorders.
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http://dx.doi.org/10.2174/1872208315666210921095420DOI Listing
September 2021

Caspase-3: Structure, function, and biotechnological aspects.

Biotechnol Appl Biochem 2021 Aug 3. Epub 2021 Aug 3.

Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Caspase-3, a cysteine-aspartic acid protease, has recently attracted much attention because of its incredible roles in tissue differentiation, regeneration, and neural development. This enzyme is a key zymogen in cell apoptosis and is not activated until it is cleaved by initiator caspases during apoptotic flux. Since caspase-3 has represented valuable capabilities in the field of medical research, biotechnological aspects of this enzyme, including the production of recombinant type, protein engineering, and designing delivery systems, have been considered as emerging therapeutic strategies in treating the apoptosis-related disorders. To date, several advances have been made in the therapeutic use of caspase-3 in the management of some diseases such as cancers, heart failure, and neurodegenerative disorders. In the current review, we intend to discuss the caspase-3's structure, functions, therapeutic applications, as well as its molecular cloning, protein engineering, and relevant delivery systems.
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http://dx.doi.org/10.1002/bab.2233DOI Listing
August 2021

Effective stiffness, damping and mass of the body during laboratory simulations of shoulder checks in ice hockey.

Sports Biomech 2021 Jul 28:1-12. Epub 2021 Jul 28.

Department of Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, BC, Canada.

Ice hockey is a fast-paced sport with a high incidence of collisions between players. Shoulder checks are especially common, accounting for a large portion of injuries including concussions. The forces generated during these collisions depend on the inertial and viscoelastic characteristics of the impacting bodies. Furthermore, the effect of shoulder pads in reducing peak force depends on the baseline (unpadded) properties of the shoulder. We conducted experiments with nine men's ice hockey players (aged 19-26) to measure their effective shoulder stiffness, damping and mass during the impact stage of a shoulder check. Participants delivered a style of check commonly observed in men's university ice hockey, involving lateral impact to the deltoid region, with the shoulder brought stationary by the collision. The effective stiffness and damping coefficient of the shoulder averaged 12.8 kN/m and 377 N-s/m at 550 N, and the effective mass averaged 47% of total body mass. The damping coefficient and stiffness increased with increasing force, but there was no significant difference in the damping coefficient above 350 N. Our results provide new evidence on the dynamics of shoulder checks in ice hockey, as a starting point for designing test systems for evaluating and improving the protective value of shoulder pads.
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http://dx.doi.org/10.1080/14763141.2021.1951828DOI Listing
July 2021

Exosomal noncoding RNAs: key players in glioblastoma drug resistance.

Mol Cell Biochem 2021 Nov 17;476(11):4081-4092. Epub 2021 Jul 17.

Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Glioma, as one of the most severe human malignancies, is defined as the Central Nervous System's (CNS) tumors. Glioblastoma (GBM) in this regard, is the most malignant type of gliomas. There are multiple therapeutic strategies to cure GBM, for which chemotherapy is often the first-line treatment. Still, various cellular processes, such as uncontrolled proliferation, invasion and metastasis, may disturb the treatment efficacy. Drug resistance is another process in this way, which can also cause undesirable effects. Thereupon, identifying the mechanisms, involved in developing drug resistance and the relevant mechanisms can be very helpful in GBM management. The discovery of exosomal non-coding RNAs (ncRNAs), RNA molecules that can be transferred between the cells and different tissues using the exosomes, was a milestone in this regard. It has been revealed that the key exosomal ncRNAs, including circular RNAs, microRNAs, and long ncRNAs, are able to modulate GBM drug resistance through different signaling pathways or by affecting regulatory proteins and their corresponding genes. Nowadays, researchers are trying to overcome the limitations of chemotherapy by targeting these RNA molecules. Accordingly, this review aims to clarify the substantial roles of exosomal ncRNAs in GBM drug resistance and involved mechanisms.
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http://dx.doi.org/10.1007/s11010-021-04221-2DOI Listing
November 2021

Glucose oxidase: Applications, sources, and recombinant production.

Biotechnol Appl Biochem 2021 Apr 11. Epub 2021 Apr 11.

Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons from an oxidant to a reductant. Glucose oxidases use oxygen as an external electron acceptor that releases hydrogen peroxide (H O ). Glucose oxidase has many applications in commercial processes, including improving the color and taste, increasing the persistence of food materials, removing the glucose from the dried egg, and eliminating the oxygen from different juices and beverages. Moreover, glucose oxidase, along with catalase, is used in glucose testing kits (especially in biosensors) to detect and measure the presence of glucose in industrial and biological solutions (e.g., blood and urine specimens). Hence, glucose oxidase is a valuable enzyme in the industry and medical diagnostics. Therefore, evaluating the structure and function of glucose oxidase is crucial for modifying as well as improving its catalytic properties. Finding different sources of glucose oxidase is an effective way to find the type of enzyme with the desired catalysis. Besides, the recombinant production of glucose oxidase is the best approach to produce sufficient amounts of glucose oxidase for various uses. Accordingly, the study of various aspects of glucose oxidase in biotechnology and bioprocessing is crucial.
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http://dx.doi.org/10.1002/bab.2165DOI Listing
April 2021
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