Publications by authors named "Olga I Nedashkovskaya"

81 Publications

Aquimarina algiphila sp. nov., a chitin degrading bacterium isolated from the red alga Tichocarpus crinitus.

Int J Syst Evol Microbiol 2018 Mar 29;68(3):892-898. Epub 2018 Jan 29.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-orange pigmented flavobacterium, designated strain 9Alg 151, was isolated from the Pacific red alga Tichocarpus crinitus. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain fell into the genus Aquimarina of the family Flavobacteriaceae with a 16S rRNA gene sequence similarity range of 94.2-98.2 % to the recognized species of the genus. Strain 9Alg 151 grew in the presence of 0.5-5 % NaCl and at 5-34 °C, and hydrolysed aesculin, agar, gelatin, starch, Tween 40, DNA and chitin. The predominant fatty acids were iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C16 : 0, iso-C17 : 1ω8c and summed feature 3. The polar lipid profile comprised phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. The major respiratory quinone was MK-6. The genomic DNA G+C content was 32.6 mol%. On the basis of 16S rRNA gene sequence data, and chemotaxonomic and phenotypic characteristics, strain 9Alg 151 represents a novel species of the genus Aquimarina, for which the name Aquimarina algiphila sp. nov. is proposed. The type strain is 9Alg 151 (=KCTC 23622=KMM 6462).
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http://dx.doi.org/10.1099/ijsem.0.002606DOI Listing
March 2018

Polaribacter staleyi sp. nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.

Int J Syst Evol Microbiol 2018 Feb 5;68(2):623-629. Epub 2018 Jan 5.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139, was isolated from the Pacific red alga Ahnfeltiato buchiensis. The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938 (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species. The prevalent fatty acids of strain 10Alg 139 were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids. The main respiratory quinone was menaquinone 6. The DNA G+C content of the type strain is 31.8 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp. nov. is proposed. The type strain is 10Alg 139 (=KCTC 52773=KMM 6729).
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http://dx.doi.org/10.1099/ijsem.0.002554DOI Listing
February 2018

Olleya algicola sp. nov., a marine bacterium isolated from the green alga Ulva fenestrata.

Int J Syst Evol Microbiol 2017 Jul 12;67(7):2205-2210. Epub 2017 Jul 12.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 3Alg 18T, was isolated from the Pacific green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was affiliated to the family Flavobacteriaceae of the phylum Bacteroidetes, being most closely related to the type strains of recognized species of the genus Olleya, with 16S rRNA gene sequence similarity of 97.9-99.3 %. Strain 3Alg 18T grew in the presence of 0.5-5 % (w/v) NaCl and at 4-37 °C, and hydrolysed aesculin, casein, gelatin, starch and Tweens 20, 40 and 80. The prevalent fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C16:0 2-OH, iso-C17 : 0 3-OH, summed feature 3, iso-C16 : 0 3-OH, anteiso-C15 : 0 and C15 : 0. The polar lipid profile contained phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major respiratory quinone was menaquinone MK-6. The genomic DNA G+C content was 34.6 mol%. On the basis of 16S rRNA gene sequence data, and chemotaxonomic and phenotypic characteristics, strain 3Alg 18T represents a novel species of the genus Olleya, for which the name Olleya algicola sp. nov. is proposed. The type strain is 3Alg 18T (=KCTC 22024T=KMM 6133T).
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http://dx.doi.org/10.1099/ijsem.0.001926DOI Listing
July 2017

Lacinutrix cladophorae sp. nov., a flavobacterium isolated from the green alga Cladophora stimpsonii, transfer of Flavirhabdus iliipiscaria Shakeela et al. 2015 to the genus Lacinutrix as Lacinutrix iliipiscaria comb. nov. and emended description of the genus Lacinutrix.

Int J Syst Evol Microbiol 2016 Nov 28;66(11):4339-4346. Epub 2016 Jul 28.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 7Alg 4T, was isolated from the green alga Cladophora stimpsonii. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain was affiliated to the family Flavobacteriaceae of the phylum Bacteroidetes, and was most closely related to the recognized species of the genera Lacinutrixand Flavirhabdus, with 16S rRNA gene sequence similarities of 95.1-98.1 and 97.0 %, respectively. Strain 7Alg 4T grew in the presence of 1-5 % NaCl and at 4-32 °C, and hydrolysed aesculin, gelatin, starch and Tween 80. The prevalent fatty acids were iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and C15 : 0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major respiratory quinone was MK-6. The DNA G+C content was 31.9 mol%. On the basis of the differences in 16S rRNA gene sequences, chemotaxonomic and phenotypic characteristics, it is suggested that strain 7Alg 4T represents a novel species of the genus Lacinutrix, for which the name Lacinutrixcladophorae sp. nov. is proposed. The type strain is 7Alg 4T (=KCTC 23036T=KMM 6381T). Reclassification of Flavirhabdus iliipiscaria as Lacinutrix iliipiscaria comb. nov. and an emend of the genus Lacinutrix are also proposed.
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http://dx.doi.org/10.1099/ijsem.0.001353DOI Listing
November 2016

Amylibacter ulvae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Ulva fenestrata.

Arch Microbiol 2016 Apr 13;198(3):251-6. Epub 2016 Jan 13.

Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon, 305-764, Republic of Korea.

A strictly aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain 6Alg 255(T), was isolated from the green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Rhodobacteraceae of the class Alphaproteobacteria being most closely related to Amylibacter marinus LMG 28364(T) with 16S rRNA gene sequence similarity of 97.2 %. Strain 6Alg 255(T) grew with 0.5-6.0 % NaCl and at 4-33 °C, hydrolysed aesculin, casein, gelatin and urea. The DNA G + C content was 50.4 mol%. The prevalent fatty acids were C18:1 ω7c and C16:0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour was strongly supported by the differences in physiological and biochemical tests. Therefore, strain 6Alg 255(T) represents a novel species of the genus Amylibacter, for which the name Amylibacter ulvae sp. nov. is proposed. The type strain is 6Alg 255(T) (=KCTC 32465(T) = KMM 6515(T)).
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http://dx.doi.org/10.1007/s00203-015-1185-1DOI Listing
April 2016

Lutibacter holmesii sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Lutibacter.

Int J Syst Evol Microbiol 2015 Nov 14;65(11):3991-3996. Epub 2015 Aug 14.

BCCM/LMG Bacteria Collection, and Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.

Seven Gram-staining-negative, strictly aerobic, pale-yellow-pigmented, rod-shaped and non-motile strains were isolated from the sea urchin Strongylocentrotus intermedius collected from Troitsa Bay, Sea of Japan. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were affiliated with the family Flavobacteriaceae. The novel isolates showed 99.9-100 % 16S rRNA gene sequence similarity to each other and were closely related to the type strains of the recognized members of the genus Lutibacter with sequence similarities of 95.8-98.4 %. The G+C content of the genomic DNA was 35-36 mol%. DNA-DNA relatedness among the sea urchin isolates was 95-99 % and between strain KMM 6277T and its most closely related type strains, Lutibacter agarilyticus KCTC 23842T and Lutibacter litoralis JCM 13034T, was 38 and 27 %, respectively. The prevalent fatty acids were iso-C15 : 0, anteiso-C15 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c fatty acids), iso-C15 : 1 and C15 : 0. The polar lipid profile was composed of the phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. The main respiratory isoprenoid quinone was MK-6.The results of phylogenetic, phenotypic and genotypic analyses indicated that the novel strains represent a novel species within the genus Lutibacter, for which the name Lutibacter holmesii sp. nov. is proposed. The type strain is KMM 6277T ( = CCUG 62221T = LMG 26737T).
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http://dx.doi.org/10.1099/ijsem.0.000525DOI Listing
November 2015

Winogradskyella litoriviva sp. nov., isolated from coastal seawater.

Int J Syst Evol Microbiol 2015 Oct;65(10):3652-3657

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A Gram-stain-negative, facultatively anaerobic, moderately halophilic and gliding bacterium, designated KMM 6491T, was isolated from coastal seawater collected from Troitsa Bay, the Sea of Japan. Comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6491T was a member of the genus Winogradskyella, with 94.5–97.9 % sequence similarity to recognized species of the genus Winogradskyella. The DNA G+C content of strain KMM 6491T was 31.3 mol% and DNA–DNA relatedness values with the type strains of Winogradskyella echinorum, Winogradskyella damuponensis, Winogradskyella eximia and Winogradskyella pulchriflava were in range of 10–26 %. Strain KMM 6491T contained menaquinone 6 (MK-6) as the single quinone and iso-C15 : 0, iso-C15 : 1, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, C15 : 0 and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c) anteiso-C15 : 0, as the prevalent fatty acids. The major polar lipids of strain KMM 6491T were phosphatidylethanolamine, two unknown aminolipids and two unknown lipids. Strain KMM 6491T was able to grow with 0.5–7 % NaCl and at 4–34 °C. The novel strain decomposed gelatin and starch and produced acid from d-glucose, maltose, mannose, rhamnose, sucrose, fructose and glycerol. On the basis of the results of the phylogenetic and phenotypic analyses it is suggested that strain KMM 6491T represents a novel species of the genus Winogradskyella, for which the name Winogradskyella litoriviva sp. nov. is proposed. The type strain is KMM 6491T ( = KCTC 23972T = LMG 26984T).
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http://dx.doi.org/10.1099/ijsem.0.000470DOI Listing
October 2015

Flavobacterium ahnfeltiae sp. nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.

Arch Microbiol 2014 Oct 8;196(10):745-52. Epub 2014 Jul 8.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia,

A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species. The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids. The DNA G+C content of the type strain was 34.3 mol%. The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp. nov. is proposed. The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
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http://dx.doi.org/10.1007/s00203-014-1010-2DOI Listing
October 2014

Flavimarina pacifica gen. nov., sp. nov., a new marine bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Leeuwenhoekiella, Leeuwenhoekiella aequorea and Leeuwenhoekiella marinoflava.

Antonie Van Leeuwenhoek 2014 Sep 15;106(3):421-9. Epub 2014 Jun 15.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia,

A facultatively anaerobic, Gram-stain negative, rod-shaped and yellow pigmented bacterium, designated strain IDSW-73(T), was isolated from a seawater sample and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain formed a distinct phyletic line in the family Flavobacteriaceae and is most closely related to the members of the genus Leeuwenhoekiella, with 16S rRNA gene sequence similarity of 91.4-92.6 %. Strain IDSW-73(T) was found to be able to grow with 0-12 % NaCl and at 4-33 °C; and was able to hydrolyse gelatin, starch and Tweens 20, 40 and 80. The DNA G+C content was determined to be 42.2 mol%. The predominant cellular fatty acids were identified as branched-chain saturated and unsaturated and straight-chain unsaturated fatty acids such as iso-C15:0, iso-C15:1, iso-C17:1 ω9c, C15:1 ω6c, iso-C15:0 3-OH, iso-C17:0 3-OH and summed feature 3 (as defined by MIDI), comprising iso-C15:0 2-OH and/or C16:1 ω7c. The polar lipids found were phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The major respiratory quinone was identified as MK-6. The significant molecular distinctiveness between the novel isolate and its nearest neighbours were strongly supported by notable differences in physiological and biochemical tests. Therefore, strain IDSW-73(T) is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Flavimarina pacifica gen. nov., sp. nov. is proposed. The type strain is IDSW-73(T) (=KCTC 32466(T) = KMM 6759(T)). Emended descriptions of the recognized species of the genus Leeuwenhoekiella are also proposed.
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http://dx.doi.org/10.1007/s10482-014-0210-8DOI Listing
September 2014

Arenicella chitinivorans sp. nov., a gammaproteobacterium isolated from the sea urchin Strongylocentrotus intermedius.

Int J Syst Evol Microbiol 2013 Nov 14;63(Pt 11):4124-4129. Epub 2013 Jun 14.

BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.

A strictly aerobic, Gram-stain-negative, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated KMM 6208(T), was isolated from a sea urchin. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that this novel isolate was affiliated to the class Gammaproteobacteria and formed a robust cluster with Arenicella xantha KMM 3895(T) with 98.2 % 16S rRNA gene sequence similarity. Strain KMM 6208(T) grew in the presence of 0.5-5 % NaCl and at a temperature range of 4-38 °C. The isolate was oxidase-positive and hydrolysed aesculin, casein, chitin, gelatin, starch and Tweens 40 and 80. The prevalent fatty acids of strain KMM 6208(T) were C16 : 1ω7c, iso-C16 : 0, iso-C18 : 0, C18 : 1ω7c and C16 : 0. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid, and the major isoprenoid quinone was Q-8. The DNA G+C content of strain KMM 6208(T) was 46.3 mol%. The DNA-DNA relatedness value of strain KMM 6208(T) with Arenicella xantha KMM 3895(T) was 5 %. Molecular data in a combination with phenotypic findings strongly suggest inclusion of this novel strain in the genus Arenicella as a representative of a novel species for which the name Arenicella chitinivorans sp. nov. is proposed. The type strain is KMM 6208(T) ( = KCTC 12711(T) = LMG 26983(T)).
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http://dx.doi.org/10.1099/ijs.0.051599-0DOI Listing
November 2013

Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis.

Antonie Van Leeuwenhoek 2013 Jun 23;103(6):1263-9. Epub 2013 Mar 23.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A strictly aerobic, Gram-stain-negative, rod-shaped and red-orange pigmented bacterium, designated strain KMM 6395(T), was isolated from the green alga Cladophora stimpsoni and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Hyphomonadaceae of the class Alphaproteobacteria being most closely related to the type strain of the single species of the genus Litorimonas, Litorimonas taeanensis G5(T), with 16S rRNA gene sequence similarity of 96.8 %. Strain KMM 6395(T) grew with 1-5 % NaCl and at 4-35 °C, hydrolysed starch and Tween 80. The DNA G+C content was 48.7 mol%. The prevalent fatty acids were C18:1 ω7c, C19:1 ω8c and C18:1 ω7c 10-methyl. The polar lipid profile was characterized by the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and an unidentified glycolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour, L. taeanensis G5(T), were strongly supported by the differences in physiological and biochemical tests. Therefore, strain KMM 6395(T) represents a novel species of the genus Litorimonas, for which the name Litorimonas cladophorae sp. nov. is proposed. The type strain is KMM 6395(T) (=KCTC 23968(T) = LMG 26985(T)). The emended descriptions of the genus Litorimonas and L. taeaensis are also provided.
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http://dx.doi.org/10.1007/s10482-013-9906-4DOI Listing
June 2013

Polaribacter reichenbachii sp. nov.: a new marine bacterium associated with the green alga Ulva fenestrata.

Curr Microbiol 2013 Jan 28;66(1):16-21. Epub 2012 Sep 28.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.

A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 6Alg 8(T), was isolated from the common Pacific green alga Ulva fenestrata. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 97.6 % to Polaribacter dokdonensis DSW-5(T) and 92.8-96.1 % to other recognized Polaribacter species. The prevalent fatty acids of strain 6Alg 8(T) were iso-C(15:0), iso-C(15:1), iso-C(15:0) 2-OH, C(15:0) and C(15:1)ω6. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The DNA G+C content of the type strain is 31.6 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Polaribacter, for which the name Polaribacter reichenbachii sp. nov. is proposed. The type strain is 6Alg 8(T) (= KCTC 23969(T) = KMM 6386(T) = LMG 26443(T)).
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http://dx.doi.org/10.1007/s00284-012-0200-xDOI Listing
January 2013

Echinimonas agarilytica gen. nov., sp. nov., a new gammaproteobacterium isolated from the sea urchin Strongylocentrotus intermedius.

Antonie Van Leeuwenhoek 2013 Jan 4;103(1):69-77. Epub 2012 Sep 4.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

A novel Gram-negative, facultatively anaerobic and motile bacterial strain, designated KMM 6351(T), was isolated from the sea urchin Strongylocentrotus intermedius and examined using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain formed a distinct phyletic line in the class Gammaproteobacteria and was most closely related to the genera Aliivibrio, Photobacterium and Vibrio. Strain KMM 6351(T) grows at 4-40 °C and with 0.5-12 % NaCl and decomposes aesculin, agar, gelatin, starch, chitin and DNA. The DNA G+C content of the strain was determined to be 46.1 mol%. The prevalent fatty acids were found to be C(16:0), C(18:1) ω7c, C(12:0) 3-OH and summed feature 3 (comprising C(16:1) ω7c and/or iso-C(15:0) 2-OH fatty acids). The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The predominant ubiquinone was found to be Q-8. The results of the phenotypic, chemotaxonomic and genotypic analyses clearly indicated that the novel strain should be assigned to a new genus and species within the class γ-Proteobacteria for which the name Echinimonas agarilytica gen. nov., sp. nov. is proposed. The type strain is KMM 6351(T) (=KCTC 22996(T) = LMG 25420(T)).
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http://dx.doi.org/10.1007/s10482-012-9787-yDOI Listing
January 2013

Altererythrobacter troitsensis sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.

Int J Syst Evol Microbiol 2013 Jan 17;63(Pt 1):93-97. Epub 2012 Feb 17.

Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 305-764, Republic of Korea.

An aerobic, halotolerant, Gram-negative bacterium was isolated from the sea urchin Strongylocentrotus intermedius and subjected to taxonomic characterization. The strain, designated KMM 6042(T), was rod-shaped, motile and yellow-pigmented. Phylogenetic analysis indicated that the strain was most closely related to the type strain of Altererythrobacter dongtanensis, and the level of 16S rRNA gene sequence similarity between the two was 99.0 %. However, the DNA-DNA relatedness between the two strains was 34.4 ± 7.6 %. Physiological and chemotaxonomic properties clearly distinguished the novel strain from other species of the genus Altererythrobacter. It is thus evident from the phylogenetic and phenotypic analyses that strain KMM 6042(T) merits recognition as a novel species of the genus Altererythrobacter, for which the name Altererythrobacter troitsensis sp. nov. (type strain, KMM 6042(T) = KCTC 12303(T) = JCM 17037(T)) is proposed.
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http://dx.doi.org/10.1099/ijs.0.038836-0DOI Listing
January 2013

Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.

Arch Microbiol 2012 Mar 20;194(3):215-21. Epub 2011 Nov 20.

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.

A bacterial strain, designated KMM 6244(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244(T) was Bacillus decolorationis LMG 19507(T) with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA-DNA hybridization experiments revealed a low relatedness (37%) of the novel isolate with the type strain of B. decolorationis LMG 19507(T). Strain KMM 6244(T) grew at 4-45°C and with 0-12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C(15:0), iso-C(15:0), anteiso-C(17:0), C(15:0), iso-C(16:0) and iso-C(14:0). The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244(T) represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244(T) (KCTC 12718(T) = LMG 26357(T)).
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http://dx.doi.org/10.1007/s00203-011-0771-0DOI Listing
March 2012

Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia.

Int J Syst Evol Microbiol 2012 Jul 12;62(Pt 7):1450-1456. Epub 2011 Aug 12.

Far Eastern Federal University, Sukhanova St. 8, 690950 Vladivostok, Russia.

A Gram-negative, facultatively anaerobic, slightly halophilic bacterium that was motile by gliding, designated KMM 6390(T), was isolated from the coastal green alga Ulva fenestrata collected from the Sea of Japan. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6390(T) was a member of the genus Winogradskyella, with Winogradskyella echinorum KMM 6211(T) as its closest relative (97.8% 16S rRNA gene sequence similarity). 16S rRNA gene sequence similarities between the isolate and other members of the genus Winogradskyella were 95.4-96.7%. DNA-DNA relatedness between the isolate and W. echinorum KMM 6211(T) was 27%. Strain KMM 6390(T) was able to grow with 0.5-4.0% NaCl and at 4-37 °C. The isolate decomposed casein, gelatin, starch and DNA and produced acid from galactose, D-glucose, lactose and maltose. The major fatty acids were iso-C(15:0), iso-C(15:1), anteiso-C(15:0) and C(15:0). The polar lipid profile comprised phosphatidylethanolamine and two unknown aminolipids. The DNA G+C content was 34.2 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic investigations, strain KMM 6390(T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella ulvae sp. nov. is proposed; the type strain is KMM 6390(T) (=KCTC 23626(T)=LMG 26444(T)). Emended descriptions of the genus Winogradskyella and of W. thalassocola, W. echinorum, W. exilis and W. eximia are also provided.
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http://dx.doi.org/10.1099/ijs.0.032219-0DOI Listing
July 2012

Corallibacter vietnamensis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae.

Int J Syst Evol Microbiol 2012 Mar 22;62(Pt 3):569-574. Epub 2011 Apr 22.

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.

The taxonomic position of two strains of a novel heterotrophic, strictly aerobic, Gram-negative, gliding, dark-orange-pigmented bacterium, designated KMM 6217(T) and KMM 6332, was determined. The strains grew at 10-40 °C and with 0.5-8.0% NaCl. They were catalase- and oxidase-positive, produced flexirubin-type pigments and hydrolysed aesculin, gelatin, starch, Tween 80 and DNA. The predominant fatty acids were iso-C(15:1), iso-C(15:0), C(15:0), summed feature 3 (comprising iso-C(15:0) 2-OH and/or C(16:1)ω7c) and iso-C(17:0) 3-OH. The polar lipid profile comprised phosphatidylethanolamine as the major component, three unknown aminolipids and one unknown lipid. The DNA G+C content was 34.7-34.8 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that the novel isolates could be placed in the family Flavobacteriaceae. The nearest neighbours of strains KMM 6217(T) and KMM 6332 were members of the genera Bizionia, Formosa, Lacinutrix, Meridianimaribacter, Mesoflavibacter and Winogradskyella, with sequence similarities to the type strains of species of these genera of 92.8-95.2, 93.7-94.5, 94.1-94.7, 94.9, 94.6 and 93.5-94.4%, respectively. Based on phenotypic, genotypic and phylogenetic data, strains KMM 6217(T) and KMM 6332 should be classified as representatives of a novel species in a new genus in the family Flavobacteriaceae of the phylum 'Bacteroidetes', for which the name Corallibacter vietnamensis gen. nov., sp. nov. is proposed; the type strain is KMM 6217(T) (=JCM 17525(T)=KCTC 23026(T)).
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http://dx.doi.org/10.1099/ijs.0.028928-0DOI Listing
March 2012

Gramella gaetbulicola sp. nov., a member of the family Flavobacteriaceae isolated from foreshore soil.

Int J Syst Evol Microbiol 2011 Nov 10;61(Pt 11):2654-2658. Epub 2010 Dec 10.

Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.

A Gram-reaction-negative, yellow-pigmented, gliding, rod-shaped, aerobic bacterium (RA5-111(T)) was isolated from foreshore soil. The taxonomic status of the novel isolate was determined using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain RA5-111(T) could be assigned to the genus Gramella, with sequence similarities of 97.7, 97.3 and 96.2 % to the type strains of Gramella echinicola, Gramella portivictoriae and Gramella marina, respectively. Chemotaxonomic and phenotypic characteristics also supported the affiliation of strain RA5-111(T) with the genus Gramella. The genomic DNA G+C content was 39.1 mol%. The isolate contained MK-6 as the predominant menaquinone, iso-C(15 : 0), iso-C(17 : 0) 3-OH and a summed feature (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c) as major fatty acids, and phosphatidylethanolamine and unknown phospholipids as the polar lipids. DNA-DNA relatedness, phenotypic, genotypic and chemotaxonomic data clearly indicate that the isolate represents a novel species of the genus Gramella, for which the name Gramella gaetbulicola sp. nov. is proposed. The type strain is RA5-111(T) ( = KCTC 23022(T) = JCM 16528(T) = NBRC 106272(T)).
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http://dx.doi.org/10.1099/ijs.0.027599-0DOI Listing
November 2011

Gramella marina sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.

Int J Syst Evol Microbiol 2010 Dec 15;60(Pt 12):2799-2802. Epub 2010 Jan 15.

Biological Resources Center, Korea Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.

The taxonomic position of a novel Gram-stain-negative, aerobic, heterotrophic, gliding, yellow-orange-pigmented bacterium, isolated from the sea urchin Strongylocentrotus intermedius and designated strain KMM 6048(T), was established. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the family Flavobacteriaceae affiliated with recognized species of the genus Gramella, forming a distinct lineage within the genus. 16S rRNA gene sequence similarities between strain KMM 6048(T) and the type strains of species of the genus Gramella were 97.4-98.4 %. In line with representative members of the genus Gramella, strain KMM 6048(T) was oxidase- and catalase-positive, hydrolysed gelatin and starch, utilized carbohydrates and possessed a DNA G+C content of 40.0mol%. However, differentiating phenotypic traits and phylogenetic distinctiveness clearly indicated that the strain represented a novel species within the genus Gramella, for which the name Gramella marina sp. nov. is proposed; the type strain is KMM 6048(T) (=KCTC 12366(T)=LMG 25418(T)).
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http://dx.doi.org/10.1099/ijs.0.013870-0DOI Listing
December 2010

Salinimicrobium marinum sp. nov., a halophilic bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Salinimicrobium and Salinimicrobium catena.

Int J Syst Evol Microbiol 2010 Oct 13;60(Pt 10):2303-2306. Epub 2009 Nov 13.

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

Two novel heterotrophic, facultatively anaerobic, gliding and yellow-pigmented bacteria, designated strains KMM 6270(T) and KMM 6320, were isolated from different marine environments and studied using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis placed the strains within the family Flavobacteriaceae. Strains KMM 6270(T) and KMM 6320 were most closely related to the type strains of recognized species of the genus Salinimicrobium (95.0-96.6 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40-41 mol%. The strains grew with 0.5-15 % (w/v) NaCl (optimum 4 % NaCl) and at 4-41 °C (optimum 28-32 °C). Aesculin and gelatin were hydrolysed, but agar, casein, DNA and chitin were not. The phylogenetic data taken together with the results of the genotypic and phenotypic studies permit the classification of strains KMM 6270(T) and KMM 6320 as members of a novel species of the genus Salinimicrobium, for which the name Salinimicrobium marinum sp. nov. is proposed. The type strain is KMM 6270(T) (=KCTC 12719(T)=LMG 25395(T)).
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http://dx.doi.org/10.1099/ijs.0.019166-0DOI Listing
October 2010

Winogradskyella pacifica sp. nov., a marine bacterium of the family Flavobacteriaceae.

Int J Syst Evol Microbiol 2010 Aug 2;60(Pt 8):1948-1951. Epub 2009 Oct 2.

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.

A novel strain, designated KMM 6019T, was isolated from coastal seawater and subjected to taxonomic examination using a polyphasic approach. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6019T formed a distinct phyletic line within the genus Winogradskyella, a member of the family Flavobacteriaceae, phylum Bacteroidetes. The 16S rRNA gene sequence similarity between the novel isolate and the type strains of the recognized species of the genus Winogradskyella was 94.0-97.8%. Winogradskyella thalassocola KMM 3907T was the closest relative, with 97.8% sequence similarity. The predominant fatty acids of strain KMM 6019T were iso-C15:0, iso-C15:1, anteiso-C15:0, iso-C15:0 3-OH, iso-C16:0 3-OH, C15:0, iso-C17:0 3-OH and summed feature 3 comprising iso-C15:0 2-OH and/or C16:1omega7c, which are characteristic for members of the genus Winogradskyella. The DNA G+C content was 35.3 mol%. Strain KMM 6019T moved by gliding and grew with 1-5% NaCl and at 4-30 degrees C. The novel strain degraded gelatin, casein and starch and produced acid from cellobiose, d-glucose and maltose. Strain KMM 6019T could clearly be differentiated from the other Winogradskyella species by the ability to utilize inositol and sorbitol and to produce hydrogen sulphide. On the basis of phylogenetic and phenotypic findings, strain KMM 6019T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella pacifica sp. nov. is proposed. The type strain is KMM 6019T (=KCTC 22997T=LMG 22568T).
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http://dx.doi.org/10.1099/ijs.0.016329-0DOI Listing
August 2010

Reclassification of Flexibacter tractuosus (Lewin 1969) Leadbetter 1974 and 'Microscilla sericea' Lewin 1969 in the genus Marivirga gen. nov. as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov.

Int J Syst Evol Microbiol 2010 Aug 18;60(Pt 8):1858-1863. Epub 2009 Sep 18.

Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yusong, Daejon 305-333, Republic of Korea.

The taxonomic position of the misclassified strains [Flexibacter] tractuosus KCTC 2958T and '[Microscilla] sericea' LMG 13021 was studied using a polyphasic approach. The two strains shared 99.1% 16S rRNA gene sequence similarity and 28% DNA-DNA relatedness. On the basis of the phylogenetic evidence supported by genotypic and phenotypic data [Flexibacter] tractuosus KCTC 2958T and '[Microscilla] sericea' LMG 13021 are classified as two distinct species in a novel genus, Marivirga, in the family 'Flammeovirgaceae', as Marivirga tractuosa comb. nov. and Marivirga sericea nom. rev., comb. nov., with strains KCTC 2958T (=ATCC 23168T =CIP 106410T =DSM 4126T =NBRC 15989T =NCIMB 1408T =VKM B-1430T) and LMG 13021T (=ATCC 23182T =NBRC 15983T =NCIMB 1403T), respectively, as the type strains. The type species is Marivirga tractuosa.
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http://dx.doi.org/10.1099/ijs.0.016121-0DOI Listing
August 2010

Bizionia echini sp. nov., isolated from a sea urchin.

Int J Syst Evol Microbiol 2010 Apr 6;60(Pt 4):928-931. Epub 2009 Aug 6.

Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.

A bacterial strain, designated KMM 6177(T), was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile by gliding and orange-pigmented. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bizionia, a member of the family Flavobacteriaceae, with 16S rRNA gene sequence similarity of 94.9-98.6 % with recognized Bizionia species. Strain KMM 6177(T) grew at 4-39 degrees C and with 1-8 % NaCl. It produced alkaline phosphatase, catalase and oxidase and hydrolysed aesculin, gelatin, DNA and Tween 20. The predominant fatty acids were iso-C(15 : 1), iso-C(15 : 0), iso-C(15 : 0) 3-OH, iso-C(17 : 0) 3-OH and a summed feature (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c). The DNA G+C content was 34.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6177(T) represents a novel species in the genus Bizionia, for which the name Bizionia echini sp. nov. is proposed. The type strain is KMM 6177(T) (=KCTC 22015(T)=LMG 25220(T)).
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http://dx.doi.org/10.1099/ijs.0.013193-0DOI Listing
April 2010

Maribacter stanieri sp. nov., a marine bacterium of the family Flavobacteriaceae.

Int J Syst Evol Microbiol 2010 Jan 31;60(Pt 1):214-218. Epub 2009 Jul 31.

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia.

The taxonomic status of two novel heterotrophic, Gram-negative, gliding and yellow pigmented bacterial strains was established in this study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strains formed a distinct lineage within the genus Maribacter, a member of the family Flavobacteriaceae, with sequence similarities of 96.3-98.5 % to recognized species of the genus Maribacter. The maximum growth temperature of the strains was 35 degrees C and they required NaCl or seawater for growth. They hydrolysed aesculin and gelatin, reduced nitrates to nitrites and produced acid from carbohydrates. The DNA G+C contents of strains KMM 6025 and KMM 6046(T) were 36-37 mol%. On the basis of phenotypic, genotypic and phylogenetic characteristics, it is suggested that the new isolates represent a novel species of the genus Maribacter, for which the name Maribacter stanieri sp. nov. is proposed. The type strain is KMM 6046(T) (=KCTC 22023(T)=LMG 22581(T)).
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http://dx.doi.org/10.1099/ijs.0.012286-0DOI Listing
January 2010

Leeuwenhoekiella palythoae sp. nov., a new member of the family Flavobacteriaceae.

Int J Syst Evol Microbiol 2009 Dec 30;59(Pt 12):3074-7. Epub 2009 Jul 30.

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.

The taxonomic status of a novel, heterotrophic, strictly aerobic, gliding and yellow-orange-pigmented bacterium (strain KMM 6264(T)), associated with the coral Palythoa, was determined. The 16S rRNA gene sequence analysis indicated that strain KMM 6264(T) clustered with the recognized species of the genus Leeuwenhoekiella of the family Flavobacteriaceae with 96.4-98.2 % sequence similarity. DNA-DNA reassociation levels between the isolate and the type strains of Leeuwenhoekiella species were 15-22 %. The DNA G+C content was 41.2 mol%. The phylogenetic evidence and the results of genomic and phenotypic analyses showed that the isolate should be classified as a member of a novel species of the genus Leeuwenhoekiella, for which the name Leeuwenhoekiella palythoae sp. nov. is proposed. The type strain is KMM 6264(T) (=KCTC 22020(T)=LMG 24856(T)).
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http://dx.doi.org/10.1099/ijs.0.010371-0DOI Listing
December 2009

Molecular characterization and therapeutic potential of a marine bacterium Pseudoalteromonas sp. KMM 701 alpha-galactosidase.

Mar Biotechnol (NY) 2010 Feb 23;12(1):111-20. Epub 2009 Jul 23.

Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 690022, prospect 100 letya Vladivostoka, 159, Vladivostok, Russian Federation.

An alpha-galactosidase capable of converting B red blood cells into the universal blood type cells at the neutral pH was produced by a novel obligate marine bacterium strain KMM 701 (VKM B-2135 D). The organism is heterotrophic, aerobic, and halophilic and requires Na+ ions and temperature up to 34 degrees C for its growth. The strain has a unique combination of polysaccharide-degrading enzymes. Its single intracellular alpha-galactosidase exceeded other glycoside hydrolases in the level of expression up to 20-fold. The alpha-galactosidase was purified to determine the N-terminal amino acid sequences and new activities. It was found to inhibit Corynebacterium diphtheria adhesion to host buccal epithelium cell surfaces with high effectiveness. The nucleotide sequence of the homodimeric alpha-galactosidase indicates that its subunit is composed of 710 amino acid residues with a calculated Mr of 80,055. This alpha-galactosidase shares structural property with 36 family glycoside hydrolases. The properties of the enzyme are likely to be highly beneficial for medicinal purposes.
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http://dx.doi.org/10.1007/s10126-009-9205-2DOI Listing
February 2010

Winogradskyella rapida sp. nov., isolated from protein-enriched seawater.

Int J Syst Evol Microbiol 2009 Sep 15;59(Pt 9):2180-4. Epub 2009 Jul 15.

Marine Microbiology, Department of Pure and Applied Natural Sciences, University of Kalmar, SE-39182 Kalmar, Sweden.

Flavobacteria are emerging as an important group of organisms associated with the degradation of complex organic matter in aquatic environments. A novel Gram-reaction-negative, heterotrophic, rod-shaped, aerobic, yellow-pigmented and gliding bacterium, strain SCB36T, was isolated from a protein-enriched seawater sample, collected at Scripps Pier, Southern California Bight (Eastern Pacific). Analysis of the 16S rRNA gene sequence showed that the bacterium was related to members of the genus Winogradskyella within the family Flavobacteriaceae, phylum Bacteroidetes. 16S rRNA gene sequence similarity to the other Winogradskyella species was 94.5-97.1%. DNA-DNA relatedness between strain SCB36T and Winogradskyella thalassocola KMM 3907T, its closest relative in terms of 16S rRNA gene sequence similarity, was 20%. On the basis of the phylogenetic and phenotypic data, strain SCB36T represents a novel species of the genus Winogradskyella, for which the name Winogradskyella rapida sp. nov. is proposed. The type strain is SCB36T (=CECT 7392T=CCUG 56098T).
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http://dx.doi.org/10.1099/ijs.0.008334-0DOI Listing
September 2009

Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius.

Int J Syst Evol Microbiol 2009 Jun;59(Pt 6):1465-8

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.

The taxonomic position of a novel marine, yellow-pigmented bacterium, designated strain KMM 6211(T), was examined by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KMM 6211(T) is a member of the family Flavobacteriaceae, phylum Bacteroidetes. The closest relative of strain KMM 6211(T) was Winogradskyella eximia KMM 3944(T), the sequence similarity being 97.1 %. The DNA G+C content of KMM 6211(T) was 33.6 mol%. The strain was motile by gliding and grew with 1-6 % NaCl and at 4-37 degrees C. Aesculin, casein and gelatin were hydrolysed, but agar, starch, DNA and chitin were not degraded. On the basis of phylogenetic data and phenotypic differences between the isolate and recognized Winogradskyella species, strain KMM 6211(T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella echinorum sp. nov. is proposed. The type strain is KMM 6211(T) (=KCTC 22026(T)=LMG 24757(T)).
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http://dx.doi.org/10.1099/ijs.0.005421-0DOI Listing
June 2009

Reclassification of Donghaeana dokdonensis Yoon et al. 2006 as Persicivirga dokdonensis comb. nov. and emended descriptions of the genus Persicivirga and of Persicivirga xylanidelens O'Sullivan et al. 2006.

Int J Syst Evol Microbiol 2009 Apr;59(Pt 4):824-7

Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.

The accurate taxonomic position of Donghaeana dokdonensis DSW-6(T) was investigated. Phylogenetic analysis revealed that the 16S rRNA gene sequences of the strain studied and of Persicivirga xylanidelens SW256(T) shared 98.2 % similarity. On the basis of phylogenetic evidence, phenotypic data and DNA-DNA reassociation values, it is proposed that strain DSW-6(T) be reclassified as Persicivirga dokdonensis comb. nov. Emended descriptions of the genus Persicivirga and of P. xylanidelens are also proposed.
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http://dx.doi.org/10.1099/ijs.0.005116-0DOI Listing
April 2009

Pseudozobellia thermophila gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae, isolated from the green alga Ulva fenestrata.

Int J Syst Evol Microbiol 2009 Apr;59(Pt 4):806-10

Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 let Vladivostoku 159, 690022 Vladivostock, Russia.

Two novel aerobic, dark-orange-pigmented, Gram-negative bacterial strains, designated KMM 3531(T) and KMM 3953, were isolated from the green alga Ulva fenestrata. Analysis of the 16S rRNA gene sequences showed that the strains represented a novel lineage within the family Flavobacteriaceae. The most closely related genera with validly published names were Costertonia, Flagellimonas, Kriegella, Maribacter, Muricauda and Zobellia, with 16S rRNA gene sequence similarities of 93.3-91.8 %. Cells of strains KMM 3531(T) and KMM 3953 were rod-shaped, motile by gliding and grew at temperatures up to 49 degrees C. They produced acid from carbohydrates and possessed oxidase, catalase, beta-galactosidase and agarase activities. The predominant cellular fatty acids were iso-C(15 : 0,) iso-C(17 : 0) 3-OH, iso-C(15 : 1) G, summed feature 3 (comprising C(16 : 1)omega7c and/or iso-capital ES, Cyrillic(15 : 0) 2-OH), iso-C(17 : 1)omega9c and iso-C(15 : 0) 3-OH. The DNA G+C content was 47-49 mol%. On the basis of phenotypic and genotypic characteristics, strains KMM 3531(T) and KMM 3953 represent a novel genus and species, for which the name Pseudozobellia thermophila gen. nov., sp. nov. is proposed. The type strain is KMM 3531(T) (=DSM 19858(T)=JCM 11733(T)=KCTC 22016(T)).
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http://dx.doi.org/10.1099/ijs.0.004143-0DOI Listing
April 2009