Publications by authors named "Nurinnisa Öztürk"

22 Publications

  • Page 1 of 1

Altered von Willebrand Factor (vWF) and ADAMTS13 Levels in Children With Cirrhosis and Extrahepatic Portal Hypertension.

J Pediatr Hematol Oncol 2020 Dec 23;Publish Ahead of Print. Epub 2020 Dec 23.

Departments of Pediatric Gastroenterology Pediatrics Medical Biochemistry, Atatürk University School of Medicine, Erzurum Department of Pediatric Gastroenterology, Karadeniz Technical University School of Medicine, Trabzon, Turkey.

Background/aim: This study was concerned with whether vWF (von Willebrand factor) and a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13 (ADAMTS13) has altered in patients with cirrhosis and extrahepatic portal hypertension (EPH). We aimed to investigate changes to vWF and ADAMTS13 in children with cirrhosis and EPH.

Patients And Methods: This study was conducted between January and October 2019 with both cirrhosis and EPH patients and with healthy volunteers. The von Willebrand factor antigen (vWF:Ag), von Willebrand Ristocetin cofactor (vWF:RCo), and ADAMTS13 antigen and activity were studied.

Results: Twenty-eight children with cirrhosis, 16 children with EPH, and 20 healthy controls were included in the study. vWF:Ag and vWF:RCo levels were higher in patients with cirrhosis than in healthy controls (171.65±101.67 vs. 85.86±30.58, P<0.01 and 121.62±55.83 vs. 61.52±27.03, P<0.01, respectively). vWF:Ag and vWF:RCo levels were higher in patients with EPH than in healthy controls (133.93±80.13 vs. 85.86±30.58, P<0.01 and 103.18±58.55 vs. 61.52±27.03, P=0.02, respectively). The ADAMTS13 antigen and activity levels were lower in patients with cirrhosis than in healthy controls (0.58±0.23 vs. 0.97±0.15, P<0.01 and 49.91±22.43 vs. 86.51±22.07, P=0.02, respectively). The ADAMTS13 antigen and activity levels were lower in patients with EPH than in healthy controls (0.69±0.11 vs. 0.97±0.15, P=0.03; and 68.50±13.29 vs. 86.51±22.07, P=0.02, respectively). The increase in vWF and the decrease in ADAMTS13 were more pronounced in cirrhotic patients with autoimmune hepatitis (AIH) than in non-AIH patients.

Conclusions: While levels of vWF:Ag and vWF:RCo increased in children with cirrhosis and EPH, levels of the ADAMTS13 antigen and ADAMTS13 activity decreased. These alterations were more pronounced in patients with AIH-derived cirrhosis.
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http://dx.doi.org/10.1097/MPH.0000000000002038DOI Listing
December 2020

DCLK-1 LEVEL IN MULTIPLE SCLEROSIS PATIENTS AND ITS CORRELATION WITH CLINIC.

Mult Scler Relat Disord 2020 Aug 22;43:102179. Epub 2020 May 22.

Ataturk University, Faculty of Medicine, Department of Biochemistry, Erzurum/Turkey.

Introduction And Objective: There are studies supporting the disruption of axonal transport in multiple sclerosis, a neurodegenerative disease. The aim of our study was to determine Doublecortin-like kinase 1 (DCLK1) levels in multiple sclerosis patients, which play a part in axonal transport, and to investigate its correlation with the disease.

Materials And Methods: The study included 30 patients above 18 years of age with the diagnosis of multiple sclerosis and 30 healthy volunteers. The disease duration, number of annual attacks, Expanded Disability Status Scale (EDSS) scores and medications of the patients who had no history of attack within the last month were recorded. DCLK1 levels were studied in the venous blood samples taken from the patients and healthy volunteers using the ELISA method.

Results: DCLK1 levels were significantly lower in the MS patient group than in the healthy control group. There was no significant correlation between disease duration, annual number of attacks, EDSS score and DCLK1 level.

Conclusion: Low levels of DCLK1 in multiple sclerosis patients support the disruption of axonal transport. The use of agents that provide DCLK1 expression may be a treatment option to prevent neurodegeneration in multiple sclerosis patients by eliminating the disruption in axonal transport, thereby improving the clinical course of the disease and cognition.
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http://dx.doi.org/10.1016/j.msard.2020.102179DOI Listing
August 2020

The relationship with clinical course and prognosis of serum endothelin-1, angiopoietin-2, and tie-2 levels in Crimean-Congo hemorrhagic fever

Turk J Med Sci 2019 08 8;49(4):1192-1197. Epub 2019 Aug 8.

Department of Public Health, Atatürk University, School of Medicine, Erzurum, Turkey

Background/aim: Crimean-Congo hemorrhagic fever (CCHF) is a serious illness characterized by fever and hemorrhage. Endothelin-1 (ET-1), angiopoietin-2 (Ang-2), and endothelial cell-specific receptor tyrosine kinase (Tie-2) are believed to be important markers of the pathogenesis, clinical course, and prognosis of the disease. The aim of this study was to determine ET-1, Ang-2, and Tie-2 levels in adults with CCHF and investigate the associations between these markers and pathogenesis and disease course.

Materials And Methods: Sixty CCHF patients were included in the study. The patients were classified according to disease severity criteria and Ang-2, Tie-2, and ET-1 levels were compared.

Results: Mean serum ET-1 level was 36.62 ± 27.99 pg/mL in the patient group and 3.70 ± 4.71 pg/mL in the control group (P = 0.001). Mean serum Ang-2 levels were 2511.18 ± 1018.64 pg/mL in the patient group and 3570.76 ± 209.52 pg/mL in the control group (P = 0.001). Mean serum Tie-2 levels were 7.35 ± 7.75 ng/mL in the patient group and 0.67 ± 1.26 ng/mL in the control group (P = 0.001).

Conclusion: Elevated ET-1 and Tie-2 levels were associated with more severe disease course, while Ang-2 level was negatively correlated with severity in adult CCHF patients. ET-1, Tie-2, and Ang-2 levels are important prognostic parameters in CCHF and may contribute significantly to treatment and follow-up.
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http://dx.doi.org/10.3906/sag-1812-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018223PMC
August 2019

Pigment epithelial-derived factor in the lens anterior capsule of patients with senile cataract with pseudoexfoliation.

Clin Exp Optom 2020 03 15;103(2):207-211. Epub 2019 May 15.

Medical Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey.

Background: This study sought to evaluate pigment epithelial-derived factor (PEDF) levels in lens anterior capsule material taken during cataract surgery from patients with senile cataract with pseudoexfoliation.

Methods: The study included 90 eyes of 86 patients who were diagnosed with, and underwent surgery for, cataracts. Sixty of the eyes included in the study had senile cataract. Thirty eyes of 30 young patients with other forms of cataract were included as a control group. Pseudoexfoliation was present in 21 patients with senile cataract. PEDF levels in the lens anterior capsule material - extracted with capsulorhexis in the classical phacoemulsification procedure - were measured by the enzyme-linked immunosorbent assay method and compared between the groups.

Results: The PEDF level in the lens anterior capsule in the senile cataract patient group was 149.36 ± 17.46 pg/ml. A statistically significant lower level of PEDF was found in the lens anterior capsule of patients with senile cataract compared with the other groups. In the patient group with pseudoexfoliation, the PEDF level in the lens anterior capsule was found to be statistically significantly lower than the patient group without pseudoexfoliation.

Conclusion: PEDF levels decrease with senile cataract and pseudoexfoliation. These findings may clarify the pathogenesis of these conditions and point toward alternative treatment modalities.
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http://dx.doi.org/10.1111/cxo.12922DOI Listing
March 2020

Evaluation of liver function by means of serum cytokeratin 18 and hepatocyte growth factor levels in patients with obstructive jaundice.

Acta Chir Belg 2018 Jun 19;118(3):167-171. Epub 2017 Dec 19.

a Department of Biochemistry , School of Medicine, Ataturk University , Erzurum , Turkey.

Objective: To evaluate the serum levels of cytokeratin 18 (CK18) and hepatocyte growth factor (HGF) in obstructive jaundice patients before and after treatment and thereby to detect the possible role of CK18 and HGF in patients with obstructive jaundice.

Patients And Methods: Forty patients who had obstructive jaundice and 40 healthy control subjects were included in the study. Patients were treated using percutaneous, endoscopic or surgical approaches. Blood samples were obtained at the day before and 7 days after the intervention for obstructive jaundice. Serum HGF and CK18 concentrations were determined by ELISA method.

Results: There were statistically significant decreases in HGF, CK18, total bilirubin and direct bilirubin serum levels, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and alkaline phosphatase activities and white blood cell count when compared with pre-treatment levels.

Conclusion: Evaluating pre- and post-treatment serum HGF and CK18 levels suggested that there was an apoptosis in obstructive jaundice patients and this apoptosis decreased after the decompression of the biliary tract. We also demonstrated that HGF levels were altered at biliary obstruction compared to healthy controls and the levels of this biomarker also decreased after decompression of biliary obstruction. We concluded that these biomarkers can be used as predictors of liver injury in biliary obstruction.
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http://dx.doi.org/10.1080/00015458.2017.1397928DOI Listing
June 2018

A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood.

Biochem Med (Zagreb) 2017 Jun;27(2):350-377

Department of Medical Biochemistry, Kuzey Laboratories, Fatih Sultan Mehmet Hospital, Istanbul.

Introduction: A nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs).

Materials And Methods: K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. RIs were computed by the parametric method with/without applying the LAVE method.

Results: Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those RIs were derived for each manufacturer. RIs were determined from all volunteers' results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific RIs were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific RIs were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC.

Conclusions: With the novel use of a freshly prepared blood panel, manufacturer-specific RIs' were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in RIs were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.
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http://dx.doi.org/10.11613/BM.2017.038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493180PMC
June 2017

Paraoxonase and Arylesterase Levels in Behcet's Disease and Their Relations with the Disease Activity.

Biochem Genet 2017 Aug 7;55(4):335-344. Epub 2017 Apr 7.

Department of Biochemistry, Medical Faculty, Ataturk University, Erzurum, Turkey.

The aim of this study was to determine the paraoxonase (PON) and arylesterase (ARE) enzyme activity levels in Behcet's disease (BD) and to investigate whether they are associated with the disease activity. Twenty-six patients (study group) with active BD and 28 healthy controls (control group) were included in this study. While the patients who had at least one of the symptoms related to genital ulcer, skin lesions, active uveitis, arthritis, thrombophlebitis, or central nervous system involvement in addition to oral ulcers were considered as the active group, the patients who did not show clinical symptoms in the last one month due to the medical treatment were considered as the inactive group in the clinical evaluation of patients with BD. The PON and ARE levels were found to be significantly lower in the study group than the control group (p < 0.05). The PON levels of the active and inactive groups were 96.23 ± 57.84 and 112.2 ± 65.14, respectively. The ARE levels of the active and inactive groups were 30.49 ± 5.81 and 30.85 ± 6.40, respectively. No significant correlations were found between clinical findings and the activity levels of PON and ARE in the active patient group (p > 0.05). The activities of the antioxidant PON and ARE enzymes are reduced in BD. Therefore, it may be useful to add antioxidant therapy to the conventional treatment of the disease.
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http://dx.doi.org/10.1007/s10528-017-9800-2DOI Listing
August 2017

Endocan Measurement for Active Behçet Disease.

Arch Rheumatol 2017 Sep 24;32(3):197-202. Epub 2017 Mar 24.

Department of Ophtalmologia, Medical Faculty of Atatürk University Erzurum, Turkey.

Objectives: This study aims to investigate the relationships between serum endocan, vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-α) levels in active Behçet disease.

Patients And Methods: Forty patients with active Behçet disease (24 males, 16 females; mean age 37.6±8.7 years; range 20 to 50 years) and 40 healthy controls (22 males, 18 females; mean age 38.8±7.9 years; range 21 to 52 years) were included in this study. Both patient and control groups underwent a complete systemic and ophthalmic examination by the same specialist. Endocan, VEGF, and TNF-α levels were measured with an enzyme-linked immunosorbent assay kit in all subjects.

Results: Serum endocan levels were 775.2±479.3 ng/mL and 275.8±145.8 ng/mL in the patient and control groups, respectively. VEGF levels were 1768.2±900.5 pg/mL and 980.2±135.3 pg/mL in the patient and control groups, respectively. TNF-α levels were 22.4±74.3 pg/mL-1 and 11.4±16.9 pg/mL-1 in the patient and control groups, respectively. There was a statistically significant difference between groups in terms of serum endocan, VEGF, and TNF-α levels. There was a significant positive correlation between serum endocan and VEGF levels in the patient group (r=0.630; p<0.001). Also, there was a significant positive correlation between serum endocan and TNF-α levels in the patient group (r=0.713; p<0.001).

Conclusion: Serum endocan level may be a new marker in evaluation of both the prognosis and activity of Behçet disease.
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http://dx.doi.org/10.5606/ArchRheumatol.2017.6072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190961PMC
September 2017

Comparison of Cobas 6500 and Iris IQ200 fully-automated urine analyzers to manual urine microscopy.

Biochem Med (Zagreb) 2016 Oct;26(3):365-375

Ataturk University, Faculty of Medicine, Department of Medical Biochemistry, Erzurum, Turkey.

Introduction: Urine screening is achieved by either automated or manual microscopic analysis. The aim of the study was to compare Cobas 6500 and Iris IQ200 urine analyzers, and manual urine microscopic analysis.

Materials And Methods: A total of 540 urine samples sent to the laboratory for chemical and sediment analysis were analyzed on Cobas 6500 and Iris IQ200 within 1 hour from sampling. One hundred and fifty three samples were found to have pathological sediment results and were subjected to manual microscopic analysis performed by laboratory staff blinded to the study. Spearman's and Gamma statistics were used for correlation analyses, and the McNemar test for the comparison of the two automated analyzers.

Results: The comparison of Cobas u701 to the manual method yielded the following regression equations: y = - 0.12 (95% CI: - 1.09 to 0.67) + 0.78 (95% CI: 0.65 to 0.95) x for WBC and y = 0.06 (95% CI: - 0.09 to 0.25) + 0.66 (95% CI: 0.57 to 0.73) x for RBC. The comparison of IQ200 Elite to manual method the following equations: y = 0.03 (95% CI: - 1.00 to 1.00) + 0.88 (95% CI: 0.66 to 1.00) x for WBC and y = - 0.22 (95% CI: - 0.80 to 0.20) + 0.40 (95% CI: 0.32 to 0.50) x for RBC. IQ200 Elite compared to Cobas u701 yielded the following equations: y = - 0.95 (95% CI: - 2.13 to 0.11) + 1.25 (95% CI: 1.08 to 1.44) x for WBC and y = - 1.20 (95% CI: - 1.80 to -0.30) + 0. 80 (95% CI: 0.55 to 1.00) x for RBC.

Conclusions: The two analyzers showed similar performances and good compatibility to manual microscopy. However, they are still inadequate in the determination of WBC, RBC, and EC in highly-pathological samples. Thus, confirmation by manual microscopic analysis may be useful.
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http://dx.doi.org/10.11613/BM.2016.040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082210PMC
October 2016

Changes in platelet parameters in leukocytosis.

Pan Afr Med J 2016 1;24:185. Epub 2016 Jul 1.

Ataturk University Faculty of Medicine, Department of Medical Biochemistry, Erzurum, Turkey.

Introduction: In recent years, platelets are known to have a large variety of functions in many pathophysiological processes and their interaction with endothelial cells and leukocytes is known to play an important role in the pathophysiology of vascular inflammation. The aim of this study was to investigate the relationship between white blood cell count in conditions resulting in leukocytosis and platelet count and platelet parameters including mean platelet volume, platelet distribution width, and plateletcrit.

Methods: White blood cell counts count and all platelet parameters were evaluated in 341 results of normal complete blood count (of which the white blood cell counts were within reference range, group 1) and 327 results of elevated white blood cell counts count (group 2).

Results: There was a significant difference between these two groups in PLT counts and PCT values, being higher in Group 2. However, there was no statistically significant difference between two groups in MPV and PDW values. On the other hand, there were statistically significant, but weak, correlations between the WBC and platelet counts in both groups (p<0.01, r=0.235 for group 1, p<0.05, r=0.116 for group 2).

Conclusion: As a conclusion PLT count and PCT values increase in infectious conditions. This study and previous studies show that PLTs are employed in infectious conditions but the exact mechanism and the exact clinical importance of this response remains to be cleared by further studies.
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http://dx.doi.org/10.11604/pamj.2016.24.185.7510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5072879PMC
February 2017

The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin.

Lab Med 2016 Nov 4;47(4):279-282. Epub 2016 Sep 4.

Department of Clinical Chemistry, Ataturk University, Erzurum, Turkey.

Objective: To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability.

Methods: We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: see text] for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point.

Results: ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4[Formula: see text] showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature.

Conclusion: Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature.
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http://dx.doi.org/10.1093/labmed/lmw027DOI Listing
November 2016

A reference interval study for common biochemical analytes in Eastern Turkey: a comparison of a reference population with laboratory data mining.

Biochem Med (Zagreb) 2016 ;26(2):210-23

Department of Medical Biochemistry, Ataturk University, School of Medicine, Erzurum, Turkey.

Introduction: The aim of this study was to define the reference intervals (RIs) in a Turkish population living in Northeast Turkey (Erzurum) for 34 analytes using direct and indirect methods. In the present study, the regional RIs obtained were compared with other RI studies, primarily the nationwide study performed in Turkey.

Materials And Methods: For the direct method, 435 blood samples were collected from a healthy group of females (N = 218) and males (N = 217) aged between 18 and 65 years. The sera were analysed in Ataturk University hospital laboratory using Roche reagents and analysers for 34 analytes. The data from 1,366,948 records were used to calculate the indirect RIs using a modified Bhattacharya method.

Results: Significant gender-related differences were observed for 17 analytes. There were also some apparent differences between RIs derived from indirect and direct methods particularly in some analytes (e.g. gamma-glutamyltransferase, creatine kinase, LDL-cholesterol and iron). The RIs derived with the direct method for some, but not all, of the analytes were generally comparable with the RIs reported in the nationwide study and other previous studies in Turkey.There were large differences between RIs derived by the direct method and the expected values shown in the kit insert (e.g. aspartate aminotransferase, total-cholesterol, HDL-cholesterol, and vitamin B12).

Conclusions: These data provide region-specific RIs for 34 analytes determined by the direct and indirect methods. The observed differences in RIs between previous studies could be related to nutritional status and environmental factors.
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http://dx.doi.org/10.11613/BM.2016.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910277PMC
August 2016

The Frequency of Some Thrombophilic Mutations in Eastern Turkey.

Eurasian J Med 2016 Feb;48(1):2-5

Department of Biochemistry, Atatürk University School of Medicine, Erzurum, Turkey.

Objective: Factor V / Factor II / Methylenetetrahydrofolate reductase, gene polymorphisms are closely associated with thrombophilia. Regional frequencies of these mutations may show a characteristic state. The aim of our study was to evaluate the frequency of commonly seen Factor V / Factor II / Methylenetetrahydrofolate reductase gene polymorphisms in Eastern Turkey.

Materials And Methods: In 433 patients sent to the laboratory with the suspicion of thrombophilia, using whole blood samples, an automated Nucleic Acid Test was used for mutation determinations in Verigene System. The kit module was designed to detect the Factor V G1691A / Factor II G20210A / Methylenetetrahydrofolate reductase gene C677T single nucleotide polymorphisms.

Results: In 433 patients, 8.7% for Factor V G1691A polymorphisms were heterozygous genotype, 3.9% for Factor II G20210A polymorphisms were heterozygous genotype, and 43.9% for methylenetetrahydrofolate reductase 677C>T polymorphisms were heterozygous genotype and 3.0% homozygous mutation genotype.

Conclusion: Detection of these commonly seen Factor V / Factor II / Methylenetetrahydrofolate reductase single nucleotide polymorphisms can help to identify patients in high risk group and to evaluate the interaction of genetic and acquired risk factors. Our findings suggest that commonly seen thrombophilic allele mutation frequency in our region is the same as the data reported in the literature.
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http://dx.doi.org/10.5152/eurasianjmed.2015.15007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792490PMC
February 2016

Serum zinc levels in patients with iron deficiency anemia and its association with symptoms of iron deficiency anemia.

Ann Hematol 2016 Apr 2;95(5):751-6. Epub 2016 Mar 2.

Department of Biochemistry, Ataturk University Medical School, Erzurum, Turkey.

Iron deficiency anemia (IDA) is a major public health problem especially in underdeveloped and developing countries. Zinc is the co-factor of several enzymes and plays a role in iron metabolism, so zinc deficiency is associated with IDA. In this study, it was aimed to investigate the relationship of symptoms of IDA and zinc deficiency in adult IDA patients. The study included 43 IDA patients and 43 healthy control subjects. All patients were asked to provide a detailed history and were subjected to a physical examination. The hematological parameters evaluated included hemoglobin (Hb); hematocrit (Ht); red blood cell (erythrocyte) count (RBC); and red cell indices mean corpuscular volume (MCV), mean corpuscular hemoglobin (МСН), mean corpuscular hemoglobin concentration (МСНС), and red cell distribution width (RDW). Anemia was defined according to the criteria defined by the World Health Organization (WHO). Serum zinc levels were measured in the flame unit of atomic absorption spectrophotometer. Symptoms attributed to iron deficiency or depletion, defined as fatigue, cardiopulmonary symptoms, mental manifestations, epithelial manifestations, and neuromuscular symptoms, were also recorded and categorized. Serum zinc levels were lower in anemic patients (103.51 ± 34.64 μ/dL) than in the control subjects (256.92 ± 88.54 μ/dL; <0.001). Patients with zinc level <99 μ/dL had significantly more frequent mental manifestations (p < 0.001), cardiopulmonary symptoms (p = 0.004), restless leg syndrome (p = 0.016), and epithelial manifestations (p < 0.001) than patients with zinc level > 100 μ/dL. When the serum zinc level was compared with pica, no statistically significant correlation was found (p = 0.742). Zinc is a trace element that functions in several processes in the body, and zinc deficiency aggravates IDA symptoms. Measurement of zinc levels and supplementation if necessary should be considered for IDA patients.
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http://dx.doi.org/10.1007/s00277-016-2628-8DOI Listing
April 2016

The Evaluation of Serum Copeptin Levels and Some Commonly Seen Thrombophilic Mutation Prevalence in Acute Pulmonary Embolism.

Biochem Genet 2016 Jun 17;54(3):306-312. Epub 2016 Feb 17.

Department of Medical Biochemistry, Faculty of Medicine, Ataturk University Research Hospital, Ataturk University Medical School, Ataturk University, 25070, Erzurum, Turkey.

Acute pulmonary embolism (PE) is a common, emergent condition and may affect a large number of patients. Copeptin has been indicated to be a sensitive biomarker of arginine vasopressin release, and has diagnostic and prognostic value in various clinical conditions. Genetic mutations are considerable components of thrombophilic diseases, and factor II gene G20210A, (FII20210A), factor V Leiden (FVL, G1691A) and methylenetetrahydrofolate reductase gene C677T (MTHFR677T) single nucleotide polymorphisms are the most common mutations of thrombophilic diseases. In this study, serum copeptin levels were determined in patients with PE and healthy controls, and the results were discussed. The prevalence of some commonly seen thrombophilic mutations was also evaluated in patients with PE. The study included 32 patients (18 male, 14 female) with PE and 24 (13 male, 11 female) age- and gender-matched healthy controls. A significant difference in serum copeptin levels was determined between the patient and control groups (8.58 ± 4.42 and 4.07 ± 1.02 pmol/L, respectively). Heterozygous mutant genotype for FII20210A and heterozygous mutant genotype for FVL were observed in 3.1 and 9.4% of patients, respectively. Mutant genotype of 49% was determined for MTHFR677T mutations. It was concluded that copeptin may have diagnostic value for PE.
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http://dx.doi.org/10.1007/s10528-016-9720-6DOI Listing
June 2016

Serum Zinc, Copper, Magnesium and Selenium Levels in Children with Helicobacter Pylori Infection.

Eurasian J Med 2015 Jun;47(2):126-9

Department of Biochemistry, Ataturk University Faculty of Medicine, Erzurum Turkey.

Objective: Helicobacter pylori infection can cause disease from mild to severe that may be accompanied by micronutrient deficiencies. We aimed to investigate serum zinc, copper, magnesium and selenium levels in Helicobacter pylori positive children.

Materials And Methods: Thirty-four children, with chronic abdominal pain and diag-nosed to be Helicobacter pylori-positive and 20 healthy children with the same demo-graphic characteristics were included in the study. Serum zinc, copper and magnesium levels were measured in the flame unit of atomic absorption spectrophotometer, selenium levels were measured in the graphite unit of the same atomic absorption spectrophotometer.

Results: Serum zinc levels were significantly higher and serum magnesium levels were significantly lower (p<0.05) in Helicobacter pylori positive children than those of the control group. Although copper levels were lower in patient group than in control group, this difference was not statistically significant (p>0.05). There was no significant difference between serum selenium levels of two groups.

Conclusion: We concluded that in Helicobacter pylori-positive children, many trace elements and mineral metabolism may change.
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http://dx.doi.org/10.5152/eurasianjmed.2015.104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494547PMC
June 2015

The low levels of circulating hepatocyte growth factor in nephrolithiasis cases: independent from gene polymorphism.

Urolithiasis 2015 Oct 17;43(5):427-32. Epub 2015 Jun 17.

Kocaeli Karamursel State Hospital, Karamursel, Kocaeli, Turkey.

Environmental and genetic factors are important in development of nephrolithiasis. In a recent study, it has been demonstrated that hepatocyte growth factor (HGF) has an anti-apoptotic effect and thus can reduce the adhesion of calcium oxalate monohydrate crystals to renal epithelial cells. The aim of this study was to evaluate the HGF serum levels and its two gene polymorphisms and possible association of the two in patients with nephrolithiasis. One hundred and five patients with nephrolithiasis and 70 healthy volunteers with similar demographic features were included in this study. Serum HGF levels were measured, and HGF intron 13 C>A (in 102 stone patients and 68 healthy subjects) and intron 14 T>C (in 99 stone patients and 56 healthy subjects) polymorphisms were determined using real-time polymerase chain reaction with TaqMan allelic discrimination method. There were no statistically significant differences in HGF intron 13 C>A and intron 14 T>C polymorphisms between the control and patient groups (X (2) = 1.72 df = 2; p = 0.42, and X (2) = 0.68 df = 2; p = 0.71, respectively). Mean serum HGF concentration was significantly lower in the stone disease patients than in the control subjects (1.05 ± 0.63 pg/mL and 1.35 ± 0.58 ng/mL respectively, p = 0.0001). When allele distribution frequency between stone patients and healthy subjects was compared, there were no significant differences in intron 13 and intron 14 allele distributions between two groups (p = 0.43 and p = 0.44, respectively). It may be concluded from the findings that decrease in HGF levels may play a role in renal stone formation, independent from gene polymorphisms.
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http://dx.doi.org/10.1007/s00240-015-0793-1DOI Listing
October 2015

S-100beta and neuron-specific enolase levels in carbon monoxide-related brain injury.

Am J Emerg Med 2010 Jan;28(1):61-7

Department of Emergency Medicine, Ataturk University, Medical School, 25090 Erzurum, Turkey.

Introduction: Carbon monoxide (CO) toxicity may cause persistent injuries in tissues sensitive to hypoxia. Neuropsychiatric sequelae may be observed in about 67% of cases after severe CO exposure.

Aim: The aims of this study were to demonstrate the usefulness of S-100beta and neuron-specific enolase (NSE) in CO intoxications, show the degree of neurological response, and determine the indications for hyperbaric oxygen treatment (HBOT) as biochemical markers.

Results: The S-100beta and NSE levels of the sera of 30 patients were studied upon admittance and at the third and sixth hours. S-100beta levels were found to be high in all 3 analyses. There was no significant change in NSE levels. When the S-100beta levels were compared with Glasgow Coma Scale levels, a strong negative correlation was found for all hours (r = -0.7, -0.8; P = .00). The correlation between S-100beta and carboxyhemoglobin levels at the initial hour was found to be statistically significant (r = 0.4; P = .01). The S-100beta levels in patients receiving HBOT showed a considerable decrease compared with those in patients not receiving the treatment. The same decrease was valid for NSE, although it was insignificant.

Conclusion: S-100beta may be useful in evaluating intoxications as an early biochemical marker in CO intoxications, as well as in the differential diagnosis due to other causes, and in determining HBOT indications.
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http://dx.doi.org/10.1016/j.ajem.2008.10.032DOI Listing
January 2010

Fetuin-A gene polymorphism in patients with calcium oxalate stone disease.

Urology 2010 Apr 22;75(4):928-32. Epub 2009 Nov 22.

Department of Biochemistry, Ataturk University Medical School, Erzurum, Turkey.

Objectives: To evaluate the association of fetuin-A polymorphisms with calcium oxalate nephrolithiasis. Fetuin-A is a circulating calcium-regulatory glycoprotein that inhibits extraosseous calcification.

Methods: Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with calcium oxalate nephrolithiasis and 73 age- and gender-matched healthy volunteers, using polymerase chain reaction-restriction fragment length polymorphism techniques. Additionally, we compared serum fetuin-A levels in the 2 groups.

Results: A statistically significant difference was observed between the control and patient groups (chi(2) test, P = .003) for the genotype of fetuin-A c.766C > G polymorphism. The odds ratio (95% confidence interval) for the CG genotype in those at risk of stone disease was 4.2 (1.73-10.28). The frequency distribution for fetuin-A c.742C > T polymorphism was not statistically different in stone patients and controls (P = .77). Serum mean fetuin-A concentration was significantly lower in the patients (710.38 +/- 156.42 microg/mL) than in the controls (810.89 +/- 173.43 microg/mL, P = .0001). In the patient group (but not in the control group), subjects carrying fetuin-A genotype 1 had significantly higher serum fetuin-A concentrations than the group carrying fetuin-A genotype 2-1 (P = .001).

Conclusions: These results reveal that the patients with fetuin-A c.766C > G gene polymorphism may be at higher risk for renal calcium oxalate stone formation.
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http://dx.doi.org/10.1016/j.urology.2009.08.058DOI Listing
April 2010

Hepatocellular damage following burn injury demonstrated by a more sensitive marker: alpha-glutathione S-transferase.

J Burn Care Res 2009 Jul-Aug;30(4):711-6

Department of General Surgery, School of Medicine, Atatürk University, Erzurum, Turkey.

Following burn injury, some complex reactions are initiated that are mainly managed by the liver and that can cause injury at the liver. Alpha glutathione S-transferase (alpha-GST) is a sensitive marker that is very sensitive in the monitoring of hepatocellular damage. We tried, in this study, to demonstrate liver injury in burn patients using alpha-GST. Forty-four patients with burn injury treated at the Burn Treatment and Care unit of the Atatürk University Medical School between July 2006 and July 2007 were included in the study. Patient data were collected. Three blood samples were taken from the patients (at admittance [first sample], 120 hours after admittance [second sample], and on the fourteenth day [third sample]) for the analysis of alpha-GST, alanine amino transferase, aspartate amino transferase activities, and albumin and c-reactive protein levels. There was a statistically significant difference between alpha-GST activities of the study group at admission (P<.001), on the fifth day (P<.001), and the 14th day (P<.001) and those of the control group. There was a decrease in alpha-GST activities during the hospitalization period. Alanine amino transferase and aspartate amino transferase activities in all three samples of the study group were not different from each other and from the values obtained from the control group. The albumin levels of the study group were significantly different from those of the control group. The c-reactive protein levels of the study group were different from those of the control group at admission, on the fifth day, and fourteenth day (P<.001, P<.001, and P<.01). Our findings suggest that burn injury causes liver injury, and alpha-GST can be used to demonstrate this.
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http://dx.doi.org/10.1097/BCR.0b013e3181abfd65DOI Listing
August 2009

Paraoxonase and arylesterase activities in patients with preeclampsia.

Eurasian J Med 2008 Apr;40(1):10-3

Atatürk University, Faculty of Medicine, Department of Biochemistry, Erzurum, Turkey.

Objective: Paraoxonase is a high density lipoprotein-associated anti-oxidant enzyme. The aim of this study was to investigate the serum paraoxonase and arylesterase activities and associations between these enzymes and lipid hydroperoxide (LOOH) in preeclamptic patients.

Materials And Methods: The study included 47 preeclamptic (25 severe and 22 mild preeclampsia cases) and 20 normotensive healthy pregnant women. Serum paraoxonase and arylesterase activities and LOOH levels were measured.

Results: While serum paraoxonase and arylesterase activities were significantly lower in both severe and mild preeclamptic patients (P<0.05 for both) than those in normal pregnant subjects, LOOH levels were significantly higher in severe (P<0.01) and mild preeclampsia cases (P<0.05) than in normotensive pregnant subjects. When all subjects were evaluated, there was a negative correlation between paraoxonase and LOOH (r=-0.24, P<0.05) and between arylesterase and LOOH (r=-0.31, P<0.01).

Conclusion: Our results show that serum paraoxonase and arylesterase activities decrease in preeclampsia, and this situation may be associated with increased LOOH in these patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261318PMC
April 2008

Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats.

Chem Biol Interact 2007 Oct 4;170(1):40-8. Epub 2007 Jul 4.

Atatürk University, Faculty of Pharmacy, Department of Pharmacology, 25240, Erzurum, Turkey.

This study investigated the effects of Onosma armeniacum K. (Boraginaceae) root extract (AR-1) on ethanol-induced stomach ulcers, and on some oxidant and antioxidant parameters, in stomach tissue in rats. The results obtained showed that AR-1 significantly inhibited ethanol-induced ulcers at 25, 50, 100 and 200 mg/kg doses. We found that 50, 100 and 200 mg/kg doses of AR-1 inhibited ulcers more effectively than did ranitidine. AR-1 at doses of 25, 50, 100 and 200 mg/kg significantly prevented the decrease in total glutathione (tGSH) level which occurs in damaged stomach tissues of rats given ethanol (control group). Only a 100 mg/kg dose of AR-1 significantly increased the glutathione S-transferase (GST) level in stomach tissue compared to the control. All doses of AR-1 except the 25 mg/kg dose eliminated the decrease in the superoxide dismutase (SOD) level in the stomach tissue of rats given ethanol. While all doses of AR-1 decreased malondialdehyde (MDA) levels significantly; all doses AR-1 except 25 mg/kg decreased myeloperoxidase (MPO) levels significantly compared to the control. The effect of AR-1 on catalase (CAT) activity was insignificant at all doses. AR-1 significantly increased nitric oxide (NO) levels at 50, 100 and 200 mg/kg doses compared to the control. Our results indicate that the protection of some antioxidant mechanisms and the inhibition of some oxidant mechanisms have a role in AR-1's antiulcer effect mechanism.
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http://dx.doi.org/10.1016/j.cbi.2007.06.040DOI Listing
October 2007