Publications by authors named "Nurdin Saidi"

7 Publications

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Novel thermostable lipase produced by a thermo-halophilic bacterium that catalyses hydrolytic and transesterification reactions.

Heliyon 2020 Jul 24;6(7):e04520. Epub 2020 Jul 24.

Biomolecules Application Research Group, Chemistry Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, Indonesia.

Lipase belongs to the class of hydrolytic enzymes that are widely used in the biotechnology industries. The goal of this research was to purify and characterize lipase produced from a thermo-halophilic bacterium, namely Pria Laot Sabang 80 (PLS 80). Purification was performed using ammonium sulphate fractionation, followed by gel filtration chromatography using Sepharose Cl-6B. After purification, the enzyme had a specific activity of 326.6 U/mg with a purity of 6.02 higher than the crude extract; with a molecular weight of around 50 kDa. The optimum activity was observed at 70 °C and pH 9. The activity increased in the presence of 10 mM Mn, K and Ca ions, while Hg only slightly increased the enzyme activity. In contrast, the activity decreased in 10 mM Mg, Zn, Co, EDTA, and PMSF. The enzyme showed good hydrolytic activity on long fatty acids substrates (-nitrophenyl palmitate) with a value of 35.5 U/mL. It was also able to catalyze a transesterification reaction. GC-MS result showed that the biodiesel consisted of methyl octanoate (5.3%), methyl caprate (12.4%), methyl laurate (34.1%), methyl myristate (10.7%), methyl palmitate (3.9%), and methyl stearate (1.2%) when using coconut oil as the substrate. The results suggested that the lipase from PLS 80 had unique attributes that could be useful in various industrial applications.
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http://dx.doi.org/10.1016/j.heliyon.2020.e04520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385455PMC
July 2020

Lignan compound isolated from n-Hexane extract myristica fragrans Houtt root as antioxidant and antitumor activities against MCF-7 cell lines data.

Data Brief 2020 Aug 5;31:105997. Epub 2020 Jul 5.

Research Centre for Chemistry, Indonesian Institute of Sciences (LIPI), Kawasan Puspiptek Serpong, Tangerang Selatan, Banten, Indonesia.

Nutmeg plant (Myristica fragrans Houtt) is known as one of traditional medicine. The nutmeg root has a strong potential in antioxidant and anticancer agents among other nutmeg plant parts. The n-hexane root extract has been carried out by thin-layer chromatography and obtained 8 fractions (labeled as Myristica fragrans Houtt Root: MFHR 1 - 8). Specifically, the MFHR 4 has been purified for several times to obtain a yellow-brown color. Furthermore, lignan compound 6'-methyl-(7‑hydroxy-8-methylbut-9-en)-3,2'-dimethoxybiphenyl-4,5-diol) was identified with chemical formula of CHO and analyzed using UV-vis spectroscopy, fourier-transform infrared spectroscopy (FTIR), 1D/2D nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Based on MTT assay, MFHR demonstrated moderate anticancer activity against MCF-7 cell lines of 51.95 µM, meanwhile, DPPH activity confirmed the strong antioxidant activity with IC50 value of 12.67 ppm.
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http://dx.doi.org/10.1016/j.dib.2020.105997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387772PMC
August 2020

Macrophylloflavone: A New Biflavonoid from Mart. (Clusiaceae) for Antibacterial, Antioxidant, and Anti-Type 2 Diabetes Mellitus Activities.

ScientificWorldJournal 2020 12;2020:2983129. Epub 2020 May 12.

Department of Chemistry Education, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.

Investigations of antibacterial, antioxidant, and anti-type 2 diabetes mellitus activities have been carried out on Mart. plant extract fractions. An isolate from a fraction of ethyl acetate extract was characterized with spectroscopic data. A new biflavonoid compound was found to have a skeleton of 5,7,4',5″,7″,3‴,4‴-heptahydroxyflavanone[3-6″] flavones which was named macrophylloflavone (). The compound was evaluated for its antibacterial activity against ATCC 25922 and ATCC 25923 with cephazolin as a positive control, antioxidant assay against 2,2 diphenyl-1-picrylhydrazyl (DPPH) with ascorbic acid as the positive control, and anti-type 2 diabetes mellitus treatment with metformin as a positive control. The biflavonoid compound exhibited a good inhibition for bacteria and free radical DPPH. Furthermore, biflavonoid compound treatment on the diabetic rats suggested its ability to decrease the blood glucose level. This study provided evidence that the plant has antibacterial, antioxidant, and antidiabetic properties.
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http://dx.doi.org/10.1155/2020/2983129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240784PMC
March 2021

Evaluation of cytotoxic activity from Temurui ( [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay.

J Adv Pharm Technol Res 2019 Apr-Jun;10(2):51-55

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, Indonesia.

Temurui ( [Linn.] Spreng) is a local plant of Aceh. The leaves of are used in most of the local foods as spices. Nowadays, cancer is claimed as the second deadly disease in the world where the number of sufferers increases every year. Cervical cancer (HeLa) is one of the most dominant cancers that happen in developing country, including Indonesia. Some chemotherapeutic agents using synthetic drugs have been used to treat cancer, but they are relatively expensive and cause poisoning that limits their use. Based on literatures, serves the potential secondary metabolites that could be developed as anticancer agent. This study aims to evaluate the cytotoxic activity from three extracts of leaves against HeLa cell line, including hexane, ethyl acetate, and methanol. Methodology used included extraction process, phytochemical screening, and cytotoxicity evaluation. The result showed that all the three extracts demonstrated a potent cytotoxic activity for HeLa cancer cells. Hexane and ethyl acetate showed a very strong cytotoxic effect with CD values <1 μg/mL, whereas methanol extract showed cytotoxic effect with CD value of 2.25 μg/mL. These results prove the potential of as an anticancer agent.
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http://dx.doi.org/10.4103/japtr.JAPTR_373_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474164PMC
May 2019

New Phenyl Propanoids from Cryptocarya bracteolata.

Nat Prod Commun 2016 Jun;11(6):815-6

Two new phenyl propanoids were extracted from the bark of Cryptocarya bracteolate Gamb., ethyl 3-(2'-hydroxy-3',4',5'-trimethoxyphenyl) propanoate (1) and ethyl 3-(2'-glucosyl-3',4',5'-trimethoxyphenyl)propanoate (2), together with seven known alkaloids, (+)-lirioferine (3), (+)-bracteoline (4), (+)-reticuline (5), (+)-reticulineN-oxide (6), (-)-norargemonine (7), (+)-bisnorargemonine (8) and atherolin (9). The structures of compounds were established through several spectroscopic methods; 1D and 2D-NMR, UV, IR and MS.
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June 2016

(6,7-Dimethoxy-4-methylisoquinolinyl)-(4'-methoxyphenyl)-methanone, a new benzylisoquinoline alkaloid from Beilschmiedia brevipes.

Molecules 2010 Mar 31;15(4):2339-46. Epub 2010 Mar 31.

Department of Chemistry, Faculty of Science and Technology, University Airlangga, Surabaya, Indonesia.

The leaves of Beilschmiedia brevipes provided a new benzylisoquinoline alkaloid: (6,7-dimethoxy-4-methylisoquinolinyl)-(4'-methoxyphenyl)-methanone (1) and O,O-dimethylannocherin A (2), a new natural compound which has been synthesized before. Complete 1H- and 13C-NMR data of both compounds were reported. The structures were established through various spectroscopic methods notably 1D- and 2D-NMR, UV, IR and HRESIMS.
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http://dx.doi.org/10.3390/molecules15042339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257239PMC
March 2010

New phenantrene alkaloids from Cryptocarya crassinervia.

Fitoterapia 2008 Jun 9;79(4):308-10. Epub 2008 Feb 9.

Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

The bark of Cryptocarya crassinervia provided two new phenantrene alkaloids, 2-hydroxyatherosperminine (1) and N-demethyl-2-methoxyatherosperminine (2).
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http://dx.doi.org/10.1016/j.fitote.2007.11.025DOI Listing
June 2008
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