Dr Nuno Bandeira, PhD - University of California

Dr Nuno Bandeira

PhD

University of California

United States

Dr Nuno Bandeira, PhD - University of California

Dr Nuno Bandeira

PhD

Introduction

Primary Affiliation: University of California - United States

Publications

55Publications

970Reads

-Profile Views

2044PubMed Central Citations

The generating function approach for Peptide identification in spectral networks.

J Comput Biol 2015 May 25;22(5):353-66. Epub 2014 Nov 25.

1 Department of Computer Science and Engineering, University of California-San Diego , La Jolla, California.

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http://dx.doi.org/10.1089/cmb.2014.0165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425220PMC
May 2015
2 Reads
3 Citations
1.740 Impact Factor

Highly Efficient Chirality Transfer from Diamines Encapsulated within a Self-Assembled Calixarene-Salen Host.

Chemistry 2015 May 24;21(19):7144-50. Epub 2015 Mar 24.

Institute of Chemical Research of Catalonia (ICIQ), Av. Països Catalans 16, 43007 Tarragona (Spain).

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http://dx.doi.org/10.1002/chem.201500333DOI Listing
May 2015
47 Reads
2 Citations
5.731 Impact Factor

Molecular networking and pattern-based genome mining improves discovery of biosynthetic gene clusters and their products from Salinispora species.

Chem Biol 2015 Apr 9;22(4):460-471. Epub 2015 Apr 9.

Center for Marine Biotechnology & Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA. Electronic address:

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http://www.cell.com/chemistry-biology/pdf/S1074-5521(15)0010
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http://linkinghub.elsevier.com/retrieve/pii/S107455211500103
Publisher Site
http://dx.doi.org/10.1016/j.chembiol.2015.03.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4409930PMC
April 2015
25 Reads
28 Citations
6.645 Impact Factor

Molecular cartography of the human skin surface in 3D.

Proc Natl Acad Sci U S A 2015 Apr 30;112(17):E2120-9. Epub 2015 Mar 30.

Skaggs School of Pharmacy and Pharmaceutical Sciences, Biochemistry, Pharmacology, and Chemistry, University of California, San Diego, La Jolla, CA 92037;

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http://dx.doi.org/10.1073/pnas.1424409112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418856PMC
April 2015
18 Reads
62 Citations
9.810 Impact Factor

MixGF: spectral probabilities for mixture spectra from more than one peptide.

Mol Cell Proteomics 2014 Dec 15;13(12):3688-97. Epub 2014 Sep 15.

§Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California; ¶Center for Computational Mass Spectrometry, University of California, San Diego, La, Jolla, California; ‖Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California 92092

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http://dx.doi.org/10.1074/mcp.O113.037218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256515PMC
December 2014
3 Reads
8 Citations
6.564 Impact Factor

Phosphinothiolates as ligands for polyhydrido copper nanoclusters.

Chemistry 2014 Dec 5;20(49):16121-7. Epub 2014 Oct 5.

Institute of Chemical Research of Catalonia (ICIQ), Avinguda Països Catalans 16, 43007 Tarragona (Spain).

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http://dx.doi.org/10.1002/chem.201404763DOI Listing
December 2014
11 Reads
5.731 Impact Factor

Automated genome mining of ribosomal peptide natural products.

ACS Chem Biol 2014 Jul 23;9(7):1545-51. Epub 2014 May 23.

Department of Electrical and Computer Engineering, University of California San Diego , La Jolla, California 92093, United States.

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http://dx.doi.org/10.1021/cb500199hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215869PMC
July 2014
32 Reads
29 Citations
5.331 Impact Factor

Combinatorial approach for large-scale identification of linked peptides from tandem mass spectrometry spectra.

Mol Cell Proteomics 2014 Apr 3;13(4):1128-36. Epub 2014 Feb 3.

Bioinformatics Program, University of California, San Diego, La Jolla, California;

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http://dx.doi.org/10.1074/mcp.M113.035758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977190PMC
April 2014
2 Reads
8 Citations
6.564 Impact Factor

A turn-key approach for large-scale identification of complex posttranslational modifications.

J Proteome Res 2014 Mar 29;13(3):1190-9. Epub 2014 Jan 29.

Bioinformatics Program, ∥Skaggs School of Pharmacy and Pharmaceutical Sciences, ⊥Center for Computational Mass Spectrometry, and ¶Department of Computer Science and Engineering, University of California, San Diego , La Jolla, California 92093, United States.

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http://dx.doi.org/10.1021/pr400368uDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993922PMC
March 2014
5 Reads
1 Citation
4.250 Impact Factor

Expanding proteome coverage with orthogonal-specificity α-lytic proteases.

Mol Cell Proteomics 2014 Mar 14;13(3):823-35. Epub 2014 Jan 14.

Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Dr., La Jolla, California 92093-0378;

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http://komiveslab.ucsd.edu/reprints/meyer2.pdf
Web Search
http://www.mcponline.org/cgi/doi/10.1074/mcp.M113.034710
Publisher Site
http://dx.doi.org/10.1074/mcp.M113.034710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945911PMC
March 2014
4 Reads
11 Citations
6.564 Impact Factor

Neutron-encoded signatures enable product ion annotation from tandem mass spectra.

Mol Cell Proteomics 2013 Dec 16;12(12):3812-23. Epub 2013 Sep 16.

Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706;

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http://dx.doi.org/10.1074/mcp.M113.028951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861726PMC
December 2013
23 Reads
12 Citations
6.564 Impact Factor

Spectral library generating function for assessing spectrum-spectrum match significance.

J Proteome Res 2013 Sep 31;12(9):3944-51. Epub 2013 Jul 31.

Department of Computer Science and Engineering, Center for Computational Mass Spectrometry, CSE, and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Dr., La Jolla, California 92093, United States.

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http://dx.doi.org/10.1021/pr400230pDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766387PMC
September 2013
20 Reads
1 Citation
4.250 Impact Factor

Do π-π stacking interactions really play a role in the magnetic coupling mechanisms of [Cu2(μ2-CH3COO)2L2(H2O)2]n+ (L = heterocyclic base, n = 0, 2) complexes? An ab initio inspection.

Inorg Chem 2013 Jul 28;52(14):7980-6. Epub 2013 Jun 28.

C8-Fac. Ciências, University de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal.

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http://dx.doi.org/10.1021/ic400517zDOI Listing
July 2013
5 Reads
4.762 Impact Factor

Sequencing-grade de novo analysis of MS/MS triplets (CID/HCD/ETD) from overlapping peptides.

J Proteome Res 2013 Jun 30;12(6):2846-57. Epub 2013 May 30.

Department of Computer Science and Engineering, University of California San Diego, La Jolla, California 92093, United States.

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http://dx.doi.org/10.1021/pr400173dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591044PMC
June 2013
8 Reads
17 Citations
4.250 Impact Factor

False discovery rates in spectral identification.

BMC Bioinformatics 2012 5;13 Suppl 16:S2. Epub 2012 Nov 5.

Department of Electrical and Computer Engineering, University of California San Diego, San Diego, CA, USA.

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http://dx.doi.org/10.1186/1471-2105-13-S16-S2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489529PMC
May 2013
4 Reads
26 Citations
2.580 Impact Factor

Shotgun protein sequencing with meta-contig assembly.

Mol Cell Proteomics 2012 Oct 13;11(10):1084-96. Epub 2012 Jul 13.

Department of Computer Science and Engineering, University of California San Diego, La Jolla, California 92093, USA.

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http://dx.doi.org/10.1074/mcp.M111.015768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3494147PMC
October 2012
6 Reads
12 Citations
6.564 Impact Factor

Quantitative proteomics reveal ATM kinase-dependent exchange in DNA damage response complexes.

J Proteome Res 2012 Oct 18;11(10):4983-91. Epub 2012 Sep 18.

Medical Scientist Training Program, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States.

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http://dx.doi.org/10.1021/pr3005524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495236PMC
October 2012
10 Reads
14 Citations
4.250 Impact Factor

The spectral networks paradigm in high throughput mass spectrometry.

Mol Biosyst 2012 Oct;8(10):2535-44

Dept. Computer Science and Engineering, University of California, San Diego, USA.

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http://dx.doi.org/10.1039/c2mb25085cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3893064PMC
October 2012
3 Reads
24 Citations

Peptide identification by tandem mass spectrometry with alternate fragmentation modes.

Mol Cell Proteomics 2012 Sep 17;11(9):550-7. Epub 2012 May 17.

Department of Computer Science and Engineering, University of California, San Diego, California, USA.

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http://dx.doi.org/10.1074/mcp.R112.018556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434779PMC
September 2012
6 Reads
15 Citations
6.564 Impact Factor

Interkingdom metabolic transformations captured by microbial imaging mass spectrometry.

Proc Natl Acad Sci U S A 2012 Aug 6;109(34):13811-6. Epub 2012 Aug 6.

Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA.

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http://dx.doi.org/10.1073/pnas.1206855109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427086PMC
August 2012
16 Reads
64 Citations
9.810 Impact Factor

Mass spectral molecular networking of living microbial colonies.

Proc Natl Acad Sci U S A 2012 Jun 14;109(26):E1743-52. Epub 2012 May 14.

Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093, USA.

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http://dx.doi.org/10.1073/pnas.1203689109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3387089PMC
June 2012
26 Reads
173 Citations
9.810 Impact Factor

Calculation of magnetic couplings in hydrogen-bonded Cu(II) complexes using density functional theory.

J Phys Chem A 2012 Apr 26;116(13):3465-73. Epub 2012 Mar 26.

Université de Lyon, CNRS, Institut de Chimie de Lyon, Ecole Normale Supérieure de Lyon, France.

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http://dx.doi.org/10.1021/jp300618vDOI Listing
April 2012
3 Reads
2.693 Impact Factor

Fast multi-blind modification search through tandem mass spectrometry.

Mol Cell Proteomics 2012 Apr 20;11(4):M111.010199. Epub 2011 Dec 20.

Division of Computer Science and Engineering, Hanyang University, Seoul 133-791, Korea.

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http://dx.doi.org/10.1074/mcp.M111.010199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322561PMC
April 2012
3 Reads
34 Citations
6.564 Impact Factor

Accurate mass spectrometry based protein quantification via shared peptides.

J Comput Biol 2012 Apr 13;19(4):337-48. Epub 2012 Mar 13.

Department of Computer Science and Engineering, UC San Diego, San Diego, California, USA.

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http://dx.doi.org/10.1089/cmb.2009.0267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317402PMC
April 2012
9 Reads
14 Citations
1.740 Impact Factor

Cis-trans isomeric and polymorphic effects on the magnetic properties of water-bridged copper coordination chains.

Inorg Chem 2012 Mar 22;51(5):3094-102. Epub 2012 Feb 22.

Fakulteta za kemijo in kemijsko tehnologijo, Univerza v Ljubljani, Aškerčeva c. 5, 1000 Ljubljana, Slovenia.

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http://dx.doi.org/10.1021/ic202568yDOI Listing
March 2012
4 Reads
4.762 Impact Factor

Peptide identification by database search of mixture tandem mass spectra.

Mol Cell Proteomics 2011 Dec 23;10(12):M111.010017. Epub 2011 Aug 23.

Bioinformatics Program, University of California, San Diego, La Jolla, CA 92093, USA.

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http://dx.doi.org/10.1074/mcp.M111.010017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237077PMC
December 2011
5 Reads
11 Citations
6.564 Impact Factor

NeuroPedia: neuropeptide database and spectral library.

Bioinformatics 2011 Oct 5;27(19):2772-3. Epub 2011 Aug 5.

Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093-0744, USA.

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http://dx.doi.org/10.1093/bioinformatics/btr445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179654PMC
October 2011
1 Read
15 Citations
4.981 Impact Factor

Indenyl ring slippage in crown thioether complexes [IndMo(CO)2L]+ and C-S activation of trithiacyclononane: experimental and theoretical studies.

Dalton Trans 2011 Oct 16;40(40):10513-25. Epub 2011 Aug 16.

Instituto de Tecnologia Química e Biológica, da Universidade Nova de Lisboa, Av. da República, EAN, 2780-157, Oeiras, Portugal.

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http://dx.doi.org/10.1039/c1dt10607dDOI Listing
October 2011
6 Reads
4.200 Impact Factor

Advancing next-generation proteomics through computational research.

J Proteome Res 2011 Jul 31;10(7):2895. Epub 2011 May 31.

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http://dx.doi.org/10.1021/pr200484bDOI Listing
July 2011
1 Citation
4.250 Impact Factor

Target-decoy approach and false discovery rate: when things may go wrong.

J Am Soc Mass Spectrom 2011 Jul 5;22(7):1111-20. Epub 2011 May 5.

Bioinformatics Program, University of California San Diego, La Jolla, CA, USA.

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http://dx.doi.org/10.1007/s13361-011-0139-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220955PMC
July 2011
19 Reads
39 Citations
2.945 Impact Factor

Coexistence of intramolecular ligand-mediated and through hydrogen-bond magnetic interactions in a chain of dicopper(II) units.

Inorg Chem 2011 Jun 27;50(12):5696-705. Epub 2011 May 27.

Departament de Química Inorgànica, Universitat de Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.

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http://dx.doi.org/10.1021/ic200480dDOI Listing
June 2011
4 Reads
4.762 Impact Factor

Gapped spectral dictionaries and their applications for database searches of tandem mass spectra.

Mol Cell Proteomics 2011 Jun 28;10(6):M110.002220. Epub 2011 Mar 28.

Department of Electrical and Computer Engineering, University of California, San Diego, CA, USA.

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http://dx.doi.org/10.1074/mcp.M110.002220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108828PMC
June 2011
6 Reads
12 Citations
6.564 Impact Factor

Spectral archives: extending spectral libraries to analyze both identified and unidentified spectra.

Nat Methods 2011 May 15;8(7):587-91. Epub 2011 May 15.

Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California, USA.

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http://dx.doi.org/10.1038/nmeth.1609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128193PMC
May 2011
11 Reads
18 Citations
32.072 Impact Factor

Experimental and theoretical studies on the magnetic properties of manganese(II) compounds with mixed isocyanate and carboxylate bridges.

Chemistry 2011 Feb 5;17(6):1988-98. Epub 2011 Jan 5.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University, Shanghai, 200062, P. R. China.

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http://dx.doi.org/10.1002/chem.201002243DOI Listing
February 2011
3 Reads
5.731 Impact Factor

Protein identification by spectral networks analysis.

Authors:
Nuno Bandeira

Methods Mol Biol 2011 ;694:151-68

Center for Computational Mass Spectrometry, University of California, San Diego, La Jolla, CA, USA.

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http://dx.doi.org/10.1007/978-1-60761-977-2_11DOI Listing
February 2011
3 Reads
2 Citations

Neuropeptidomic components generated by proteomic functions in secretory vesicles for cell-cell communication.

AAPS J 2010 Dec 24;12(4):635-45. Epub 2010 Aug 24.

University of California, San Diego, La Jolla, 92093-0744, USA.

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http://dx.doi.org/10.1208/s12248-010-9223-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2976990PMC
December 2010
24 Reads
12 Citations
3.800 Impact Factor

The generating function of CID, ETD, and CID/ETD pairs of tandem mass spectra: applications to database search.

Mol Cell Proteomics 2010 Dec 9;9(12):2840-52. Epub 2010 Sep 9.

Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA 92093, USA.

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http://dx.doi.org/10.1074/mcp.M110.003731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101864PMC
December 2010
89 Reads
85 Citations
6.564 Impact Factor

Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum.

J Biol Chem 2010 Sep 25;285(36):28220-8. Epub 2010 Jun 25.

Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, California 92093, USA.

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http://nizetlab.ucsd.edu/Publications/ClostridiolysinS.pdf
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http://www.jbc.org/cgi/doi/10.1074/jbc.M110.118554
Publisher Site
http://dx.doi.org/10.1074/jbc.M110.118554DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2934687PMC
September 2010
13 Reads
28 Citations
4.573 Impact Factor

Peptide identification from mixture tandem mass spectra.

Mol Cell Proteomics 2010 Jul 27;9(7):1476-85. Epub 2010 Mar 27.

Bioinformatics Program, University of California San Diego, La Jolla, California 92093, USA.

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http://dx.doi.org/10.1074/mcp.M000136-MCP201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938093PMC
July 2010
18 Reads
20 Citations
6.564 Impact Factor

Dereplication and de novo sequencing of nonribosomal peptides.

Nat Methods 2009 Aug 13;6(8):596-9. Epub 2009 Jul 13.

Bioinformatics Program, University of California San Diego, La Jolla, California, USA.

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http://dx.doi.org/10.1038/nmeth.1350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2754211PMC
August 2009
16 Reads
31 Citations
32.072 Impact Factor

Spectral profiles, a novel representation of tandem mass spectra and their applications for de novo peptide sequencing and identification.

Mol Cell Proteomics 2009 Jun 2;8(6):1391-400. Epub 2009 Mar 2.

Department of Computer Science and Engineering, University of California San Diego, La Jolla, California 92093, USA.

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http://dx.doi.org/10.1074/mcp.M800535-MCP200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2690493PMC
June 2009
5 Reads
11 Citations
6.564 Impact Factor

Interpretation of tandem mass spectra obtained from cyclic nonribosomal peptides.

Anal Chem 2009 Jun;81(11):4200-9

Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0636, USA.

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http://dx.doi.org/10.1021/ac900114tDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765223PMC
June 2009
6 Reads
22 Citations
5.640 Impact Factor

Spectral dictionaries: Integrating de novo peptide sequencing with database search of tandem mass spectra.

Mol Cell Proteomics 2009 Jan 14;8(1):53-69. Epub 2008 Aug 14.

Department of Computer Science and Engineering, University of California San Diego, La Jolla, California 92093, USA.

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http://dx.doi.org/10.1074/mcp.M800103-MCP200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2621003PMC
January 2009
13 Reads
33 Citations
6.564 Impact Factor

Automated de novo protein sequencing of monoclonal antibodies.

Nat Biotechnol 2008 Dec;26(12):1336-8

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http://dx.doi.org/10.1038/nbt1208-1336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2891972PMC
December 2008
2 Reads
31 Citations
41.514 Impact Factor

Multi-spectra peptide sequencing and its applications to multistage mass spectrometry.

Bioinformatics 2008 Jul;24(13):i416-23

Department of Computer Science and Engineering, University of California, San Diego, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2718660PMC
http://dx.doi.org/10.1093/bioinformatics/btn184DOI Listing
July 2008
2 Reads
12 Citations
4.981 Impact Factor

Clustering millions of tandem mass spectra.

J Proteome Res 2008 Jan 8;7(1):113-22. Epub 2007 Dec 8.

Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California 92093-0404, USA.

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http://dx.doi.org/10.1021/pr070361eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533155PMC
January 2008
2 Reads
59 Citations
4.250 Impact Factor

Shotgun protein sequencing: assembly of peptide tandem mass spectra from mixtures of modified proteins.

Mol Cell Proteomics 2007 Jul 19;6(7):1123-34. Epub 2007 Apr 19.

Department of Computer Science and Engineering, University of California San Diego, La Jolla, California 92093, USA.

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http://dx.doi.org/10.1074/mcp.M700001-MCP200DOI Listing
July 2007
20 Reads
21 Citations
6.564 Impact Factor

Spectral networks: a new approach to de novo discovery of protein sequences and posttranslational modifications.

Authors:
Nuno Bandeira

Biotechniques 2007 Jun;42(6):687, 689, 691 passim

University of California, San Diego, CA, USA.

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http://dx.doi.org/10.2144/000112487DOI Listing
June 2007
4 Reads
10 Citations
2.950 Impact Factor

Protein identification by spectral networks analysis.

Proc Natl Acad Sci U S A 2007 Apr 2;104(15):6140-5. Epub 2007 Apr 2.

Department of Computer Science and Engineering, University of California at San Diego, La Jolla, CA 92093, USA.

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http://dx.doi.org/10.1073/pnas.0701130104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851064PMC
April 2007
2 Reads
61 Citations
9.810 Impact Factor

Shotgun protein sequencing by tandem mass spectra assembly.

Anal Chem 2004 Dec;76(24):7221-33

Computer Science and Engineering Department, University of California, San Diego, Department 0114, 9500 Gilman Drive, La Jolla, California 92093-0114, USA.

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http://dx.doi.org/10.1021/ac0489162DOI Listing
December 2004
2 Reads
15 Citations
5.640 Impact Factor