Publications by authors named "Novik V"

88 Publications

[Global respiratory failure as the presentation form of hypothyroidism. Report of one case].

Rev Med Chil 2004 Jan;132(1):81-4

Departamento de Endocrinología, Servicio de Medicina del Hospital Dr. Gustavo Fricke de Viña del Mar.

We report a 36 years male, admitted to the hospital for progressive respiratory failure. Chest X ray and CT scan were normal. On admission, a severe bradycardia and slow intellectual activity were noted. Serum thyroid function tests showed a TSH over 150 microU/ml and T3 of 75 ng/ml. Thyroid substitution therapy was associated with a progressive improvement of respiratory function. Diaphragmatic dysfunction, central hypoventilation, airway obstruction, sleep apnea and pleural effusion have been previously reported in patients with hypothyroidism. Therefore, we recommend to measure TSH in patients with unexplained respiratory failure.
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http://dx.doi.org/10.4067/s0034-98872004000100013DOI Listing
January 2004

[New potential of estrogen and progesterone receptor assay in cytological smears sampled from breast cancer patients].

Vopr Onkol 2004 ;50(3):355-6

Estrogen (ER) and progesterone (PR) receptors were assayed immunohistochemically in cytological smears and histological samples from 30 breast cancer patients. Since the results reported by the two research centers matched by 73%, it is suggested that running ER and PR determinations on cytological smears in cases of inoperable cancer, preoperative therapy and monitoring may be recommended. Therefore, the 3-4 year old records on ER and PR determinations should be regarded as null and void.
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September 2004

[Correlation of cytologic and DNA ploidy evidence and prognosis in childhood Wilm's tumor].

Vopr Onkol 2004 ;50(2):214-8

N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

A retrospective evaluation of the clinical features, cytological, biological (DNA) and histological data on 29 children with Wilms' tumor. Two prognosis-related groups were identified: (1) good outcome (17)--all children, aged 4-15, had survived by end of follow-up; (2) bad outcome (12)--all children died within 4 months--4 years. Cytological examination by fine-needle biopsy of smears was carried out prior to preoperative neoadjuvant therapy in all 29 patients; in addition, print smears from resected tumor were taken from 21. Blastemal, stromal, epithelial and anaplastic cells levels were estimated by the semi-quantitative procedure (0, 1+, 2+, 3+). DNA ploidy was determined by image cytometry after destaining slides and re-staining them according to Feulgen. It was shown that favorable outcome could be expected when fine-needle biopsy of print smears identified DNA diploidy and marked drug-related pathomorphosis involving fewer blastemal cells and signs of cell dystrophy and tumor necrosis. When anaplastic and aneuploid cells were detected, matched by the absence of therapeutic pathomorphosis, outcome was poor.
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July 2004

[Study of the antioxidant drug "Karinat" in patients with chronic atrophic gastritis].

Vopr Onkol 2004 ;50(1):81-5

N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

A randomized double blind placebo-controlled trial of the drug karinat was carried out in patients with chronic multifocal atrophic gastritis. Karinat contains beta-carotene 2.5 mg, alpha-tocopherol 5 mg, ascorbic acid 30 mg and garlic powder 150 mg per tablet. Out of 66 patients, 34 received karinat, 32--placebo. Both karinat and placebo were administered for 6 months, one tablet twice a day. Karinat therapy improved digestion, the fibrogastroscopic pattern of mucosa, inhibited Helicobacter pylori infection, stimulated stomach activity, mitigated intestinal metaplasia and interfered with the epithelial proliferation of gastric mucosa. These therapeutic effects were more pronounced in the study group. On the whole, the effectiveness of the drug was significantly higher (29%). Karinat should be recommended for the management of chronic atrophic gastritis, a precursor of stomach cancer.
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May 2004

[Mikhail Pavlovich Ptokhov (100th anniversary of birth)].

Authors:
V I Novik

Vopr Onkol 2003 ;49(2):245-7

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June 2003

[A comparative evaluation of treatment methods for a genital papillomavirus infection in women with different viral genotypes].

Vopr Onkol 2000 ;46(3):340-3

N. N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

Such genotypes of human papilloma virus (HPV) as "malignant" (180), "benign" (49) and indeterminate (127) were identified in 356 females by PCR and cytological procedures. The following therapeutic techniques were compared: cryodestruction of the uterine cervix (60), antiviral medication (AVM) (19), combination of AVM and cryodestruction (224), and combination of AVM and laser destruction (54). In the "malignant" HPV group, AVM in conjunction with cryo- or laser destruction was significantly more effective than cryodestruction or AVM alone. Both cryodestruction alone and in combination with AVM were more effective in treating "benign" genotypes. Repeat courses showed the same tendency.
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September 2000

[The use of computer technology in the cytologic diagnosis of neoplasms].

Authors:
V I Novik

Vopr Onkol 2000 ;46(2):239-42

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June 2000

[Investigation of nucleolar organizer region activity in epithelial cells of hyperplastic endometrial carcinoma tissue].

Vopr Onkol 2000 ;46(2):187-90

N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

Such characteristics of nucleolar organizer regions (NORs) as nucleolar and intra-nucleolar grain counts, NOR mean area, mean total NOR area in nucleus, nuclear area and nucleolus/nucleus ratio were identified using vaginal aspirate-smears from 34 patients with histologically verified diagnosis (adenocarcinoma--10, atypical hyperplasia (ATH)--10, glandular endometrial hyperplasia (GEH)--4); silver-staining and micro-imaging analyzer techniques were employed. Atypical hyperplasia and nucleolar count in nucleus progressively and significantly increased in the following sequence: proliferative phase--GEH--adenocarcinoma. Mean and total area of NORs showed a significant rise in ATH and adenocarcinoma. Those indices of endometrial cell proliferation and GEH were identical. Nuclear area and nucleolus/nucleus ratio offered most information for differentiating GEH, ATH and carcinoma. A highly reliable correlation was established between intra-nucleolar grain count and microscopically-assayed silver concentration, on the one hand, and NOR area, on the other.
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June 2000

[The results of cytologic diagnosis of bone tumors].

Vopr Onkol 1999 ;45(6):685-9

N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

The results of bone tumor cytological diagnosis in 968 patients examined and treated at the Institute Clinic (1988-1997) were analyzed. Histological examinations were performed in 479 cases. The share of cytologically inconclusive findings was significantly higher with benign lesions (17.5%) than malignant tumors (9.0%; p(0.05). The sensitivity and specificity of cytological analysis were 94.7 and 93.4%, respectively; positive predictive value--96.7, negative--89.8 and overall diagnostic accuracy--94.3%. Histological pattern of tumor was correctly identified in 73.7% of malignancies.
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March 2000

[Incidence and risk factors of papillomavirus infection and cervical dysplasia in sexually active adolescent girls].

Vopr Onkol 1999 ;45(6):623-6

N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

A cytological examination of 425 sexually active females aged 13-17 established cervical HPV infection incidence at 30.3 +/- 2.2% while that of cervical dysplasia--4.5 +/- 1.0%. The risk factors for HPV infection included low education and income level (p < 0.05), number of sexual partners exceeding three (p < 0.05), poor hygienic standards of woman (p < 0.05) and those of partner (p < 0.001) and smoking of more than 5 cigarettes per day (p < 0.001). The risk factors of cervical epithelial dysplasia were number of partners of 5 or more (p < 0.001), poor hygienic standards of male partner (p < 0.001) and smoking of 10 cigarettes and more per day (p < 0.001), etc.
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March 2000

In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria.

Proc Natl Acad Sci U S A 1999 Feb;96(4):1645-50

Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

mariner family transposons are widespread among eukaryotic organisms. These transposons are apparently horizontally transmitted among diverse eukaryotes and can also transpose in vitro in the absence of added cofactors. Here we show that transposons derived from the mariner element Himar1 can efficiently transpose in bacteria in vivo. We have developed simple transposition systems by using minitransposons, made up of short inverted repeats flanking antibiotic resistance markers. These elements can efficiently transpose after expression of transposase from an appropriate bacterial promoter. We found that transposition of mariner-based elements in Escherichia coli produces diverse insertion mutations in either a targeted plasmid or a chromosomal gene. With Himar1-derived transposons we were able to isolate phage-resistant mutants of both E. coli and Mycobacterium smegmatis. mariner-based transposons will provide valuable tools for mutagenesis and genetic manipulation of bacteria that currently lack well developed genetic systems.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC15546PMC
http://dx.doi.org/10.1073/pnas.96.4.1645DOI Listing
February 1999

Identification of multiple sigma54-dependent transcriptional activators in Vibrio cholerae.

J Bacteriol 1998 Oct;180(19):5256-9

Department of Microbiology, University of Texas Health Science Center, San Antonio, Texas 78284-7758, USA

In the pathogenic bacterium Vibrio cholerae, the alternate sigma factor sigma54 is required for expression of multiple sets of genes, including an unidentified gene(s) necessary for enhanced colonization within the host. To identify sigma54-dependent transcriptional activators involved in colonization, PCR was performed with V. cholerae chromosomal DNA and degenerate primers, revealing six novel and distinct coding sequences with homology to sigma54-dependent activators. One sequence had high homology to the luxO gene of V. harveyi, which in that organism is involved in quorum sensing. Phenotypes of V. cholerae strains containing mutations in each of the six putative sigma54-dependent activator genes identified one as a probable ntrC homologue. None of the mutant strains exhibited a defect in the ability to colonize infant mice, suggesting the presence of additional sigma54-dependent activators not identified by this technique.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107568PMC
http://dx.doi.org/10.1128/JB.180.19.5256-5259.1998DOI Listing
October 1998

[Calcium intake and bone density in menopause. Data of a sample of Chilean women followed-up for 5 years with calcium supplementation].

Rev Med Chil 1998 Feb;126(2):145-50

Departamento de Endocrinología, Escuela de Medicina, P Universidad Católica, Chile.

Background: A low calcium intake is considered a risk factor for osteoporosis.

Aim: To measure calcium intake and its relationship to bone mineral density in postmenopausal women and to assess the long term changes in bone mineral density after calcium supplementation.

Patients And Methods: In 80 postmenopausal women, older than 41 years of age, calcium intake was assessed using dietary inquiries and bone mineral density was measured using a double beam radiological densitometer. Twenty-four randomly selected women received a daily calcium supplementation of 500 mg during five years and their bone mineral density was measured at 1, 3 and 5 years.

Results: Initial calcium intake was 745 +/- 37 mg/day, and it did not change in the five-year follow up. There was no relationship between bone mineral density and calcium intake. In supplemented women, bone density did not change significantly at year one. At year three, a significant reduction was observed in the spine (-4.2%), Wards triangle (-4%) and whole body mineral content (-1.14%). At year five, there was a significant increase in bone density at the spine, femoral neck and Wards triangle as compared with year three, but not with baseline measurements. No significant differences after supplementation were observed between women with initial calcium intake of less than 500 mg/day or over this value.

Conclusions: Calcium intake in urban Chilean postmenopausal women is below recommendations and stable over time. Calcium intake and bone mineral density after the menopause are not correlated. A 500 mg/day calcium supplementation during five years is associated with a late reversal of the postmenopausal bone-losing trend.
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February 1998

[Primary multiple neoplasm of the endometrium and adjuvant hormone therapy in patients with breast cancer].

Vopr Onkol 1998 ;44(2):170-4

Prof. N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

The contribution made by adjuvant hormone therapy in the development of primary multiple endometrial adenocarcinoma (EA) in patients with breast tumors (BT) is not quite clear. The study was based on the data on 5,790 cases of BT treated at our Clinic and 4,447 females screened for hormone-dependent neoplasms. Patients with BT were found to be at high cumulative risk for endometrial carcinogenesis caused by general factors of risk and pathogenesis. The risk was particularly high in BT (stage 1) within 12 months after treatment. There was no correlation between tamoxifen treatment and significant increase in EA frequency. Promotion of tumor proliferation by tamoxifen was identified in endometrial tissue in 62 patients with BT. This may facilitate clinical manifestations of another latent EA in such patients. Dynamic surveillance of the endometrium and ovary should include ultrasonography of pelvic organs and cytologic examination (smears) of the ecto- and endocervix and endometrial aspiration biopsy.
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June 1998

[Results of cytologic diagnosis of breast tumors].

Vopr Onkol 1998 ;44(1):108-12

N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.

The data on the cytological diagnosis of breast cancer in 4.410 patients tuated at the Clinic of the Institute in 1993-1996 are presented. There was histological evidence on 1,479 patients. The sensitivity of cytological examination was 97.2, specificity--92.4, predictive value of positive test--97 and that of negative test--93%. Correlations between cytological evidence, histological pattern, stage and size of tumor are discussed.
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June 1998

[Information on a sectional meeting on the problems of cytological diagnosis].

Authors:
V I Novik

Klin Lab Diagn 1997 Feb(2):37-8

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February 1997

[The results of cytologic diagnosis of thyroid diseases (20 years experience)].

Vopr Onkol 1997 ;43(2):202-5

The data on fine-needle aspiration biopsies and imprint smears for thyroid nodules and regional lymph nodes carried out in 1129 patients at the Institute Clinic (1976-1995) were reviewed. Histological examination was performed in 340 cases. The cytological procedure showed a diagnostic sensitivity of 92.3% and a specificity of 75.8%.
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July 1997

[Milestones in the development of neoplastic cytodiagnosis].

Vopr Onkol 1997 ;43(1):78-80

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May 1997

[Severe cardiac failure as complication of primary hypothyroidism].

Rev Med Chil 1996 Oct;124(10):1248-50

Departamento de Endocrinología, Metabolismo y Nutrición, Escuela de Medicina, P Universidad Catolica de Chile.

We report a 29 years old female admitted due to a congestive cardiac failure that failed to respond to therapy with furosemide and enalapril. Serum thyroid hormone profile showed a TSH over 40 microIU/ml, a thyroxine of 0.8 microgram/dl and a triiodothyronine below 20 ng/dl. Levothyroxine therapy was started with remission of cardiac failure. The study of thyroid function in patients with cardiac failure of unknown origin and resistant to therapy, should be bone in mind.
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October 1996

[First results of accreditation of cytological services in St, Petersburg].

Authors:
V I Novik V A Kotov

Klin Lab Diagn 1996 Mar-Apr(2):3-7

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August 1996

[Possibilities of cytologic diagnosis of uterine cervix carcinoma in situ].

Authors:
V I Novik

Vopr Onkol 1996 ;42(5):109-12

The feasibility of cytological diagnosis of in situ carcinoma of the cervix uteri is discussed. A revision of the histological data and a re-evaluation of the cytological slides used have suggested cytomorphological criteria for diagnosis of the following variants of carcinoma in situ: well-differentiated squamous-cell carcinoma in situ, moderately-differentiated, low-differentiated (reserve cell), adenocarcinoma in situ (endocervical and endometroid variants). A brief description of the peculiarities of cytographs of said forms of in situ carcinoma of the cervix uteri is given. Consideration of said peculiarities will improve the standards of cytological studies involved both in screening and examination of patients.
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March 1997

[Association of papillomavirus infection and latent hyperprolactinemia in patients with dysplasia and preinvasive cancer of the cervix uteri].

Vopr Onkol 1994 ;40(1-3):53-9

Gynecological screenings carried out in I, 247 females and supplemented by cytological examination of ectoendocervical mucosa smears established significantly higher rates of detection of cytological signs of papillomavirus infection (PVI), regardless of age, in cases of background pathologies of the uterine cervix (10.0%) than in females without detectable pathology of the ectocervix (3.6%). Females of reproductive age revealed a significantly higher frequency of PVI-associated dysplasia and preinvasive carcinoma (4.2%) than in pre- and postmenopausal women (2.8%). In 24 patients with a combination of dysplasia, cervical flat condylomas and PVI signs, thyroliberin load served to identify higher levels of latent prolactin production as compared to healthy controls, the basal concentrations of blood prolactin being identical. Electrosurgical conization of the cervix was performed in 98 cases of cervical dysplasia. Morphological examination of resected tissue confirmed the relationship between PVI infection and degree of dysplasia in cervical epithelium.
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May 1995

Serum lipoprotein(a) in patients with diabetes mellitus.

Diabetes Care 1993 May;16(5):819-23

Department of Internal Medicine, Jolimont Hospital, Haine St Paul, Belgium.

Objective: To investigate subjects with different types of diabetes mellitus regarding their serum levels of lipoprotein(a). High serum Lp(a) concentration is associated with a high risk of coronary heart disease. Diabetic patients are prone to developing coronary heart disease.

Research Design And Methods: The subjects were 66 type I diabetic patients, 100 type II diabetic patients treated with diet alone or diet combined with oral hypoglycemic agents, and 46 insulin-requiring type II diabetic patients. Subjects were compared with 142 nondiabetic outpatients.

Results: Subjects with insulin-requiring type II diabetes mellitus were found to have an increase both in serum Lp(a) concentration and in prevalence of serum Lp(a) concentration > 30 mg/dl compared with the other groups of diabetic patients and nondiabetic control subjects. A nonsignificant increase in the prevalence of coronary heart disease was also found in insulin-requiring type II diabetic patients. The levels of serum concentrations of Lp(a) were not significantly related to the degree of glycemic control, duration of diabetes, presence of macrovascular disease, or intake of female hormone therapy. High levels of Lp(a) in this group of diabetic patients could not be explained by the presence of albuminuria.

Conclusions: Insulin-requiring type II diabetic patients have high levels of Lp(a). Chronic hyperinsulinemia might be an eventual causal factor.
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http://dx.doi.org/10.2337/diacare.16.5.819DOI Listing
May 1993

[Cytomorphological criteria of atypical hyperplasia and cancer of the endometrium based on data from a multivariate statistical analysis].

Vopr Onkol 1991 ;37(1):81-6

Multifactorial statistical analysis was used to identify differential diagnostic cytological features of glandular hyperplasia, atypical hyperplasia and well-differentiated adenocarcinoma of the endometrium. Twelve cytological features were identified for calculating linear discriminant function. The effectiveness of the diagnostic algorithm depends upon the diagnostic value of the sample and proficiency of the cytologist.
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May 1991

[Biometric studies in the cytological diagnosis of dysplasia and cancer of the uterine cervix].

Lab Delo 1991 (2):36-9

Morphometric parameters were measured and specific light optic features of epitheliocyte nuclei examined in the cytologic preparations from 67 patients with various abnormalities of the cervix uteri multi-layer squamous epithelium by a present-day long-distance microimage analyzer. The most informative biometric parameters were detected, role of DNA cytophotometry defined, and algorithm of differential diagnosis between benign conditions, dysplasia, and carcinoma developed.
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July 1991

[Association of idiopathic hemochromatosis and tardive cutaneous porphyria].

Acta Clin Belg 1991 ;46(5):333-7

Département de Médecine Interne, Cliniques Universitaires UCL de Mont-Godinne, Yvoir.

Porphyria cutanea tarda is a disorder of porphyrin metabolism that results from a deficiency of uroporphyrinogen decarboxylase, resulting in a characteristic pattern of porphyrin excretion. Elevated serum iron values are frequently observed among patients with porphyria cutanea tarda. The hypothesis has been advanced that a hemochromatosis allele is implicated in the clinical manifestation of porphyria cutanea tarda. We report the case of a patient suffering from both idiopathic hemochromatosis and porphyria cutanea tarda. The data of the medical literature concerning such an association are discussed.
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http://dx.doi.org/10.1080/17843286.1991.11718185DOI Listing
January 1992

[Biometric research in the cytomorphological diagnosis of endometrial lesions].

Eksp Onkol 1990 ;12(2):30-3

Morphometrical and optical density (including DNA content) parametres of nuclei of the endometrial epithelial cells in smears of endometrial aspirates obtained from 57 women without endometrial disorders with hyperplastic, precancer conditions and endometrial cancer have been measured by using television image analyzer IBAS-2. Biometrical data have demonstrated a good correlation with cytomorphological diagnostic criteria and in 85.7% they have facilitated discrimination of the normal and hyperplastic epithelium from precancer and high differentiated endometrial cancer.
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May 1990

[Cytological screening of precancer and cancer of the cervix uteri].

Authors:
V I Novik

Vopr Onkol 1990 ;36(12):1411-8

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March 1991
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