Publications by authors named "Noor Hanis Abu Halim"

4 Publications

  • Page 1 of 1

The Effects of Lentivirus-Mediated Gene Silencing of on the Stemness Capability of Non-Small Cell Lung Cancer.

J Cancer 2021 19;12(12):3468-3485. Epub 2021 Apr 19.

Lung Stem Cell and Gene Therapy Group, Regenerative Medicine Cluster, Advanced Medical and Dental Institute (IPPT), [email protected], Universiti Sains Malaysia, Kepala Batas Penang, 13200, Malaysia.

Retinoic acid receptor beta is a nuclear receptor protein that binds to retinoic acid (RA) to mediate cellular signalling in embryogenic morphogenesis, cell growth, and differentiation. However, the function of in cancer stem cells (CSCs) has yet to be determined. This study aimed to understand the role of in regulating cell growth and differentiation of lung cancer stem cells. Based on the clonogenic assay, spheroid assay, mRNA levels of stem cell transcription factors, and cell cycle being arrested at the G0/G1 phase, the suppression of resulted in significant inhibition of A549 parental cell growth. This finding was contradictory to the results seen in CSCs, where inhibition enhanced the cell growth of putative and non-putative CSCs. These results suggest that suppression may act as an essential regulator in A549 parental cells, but not in the CSCs population. The findings in this study demonstrated that the loss of promotes tumorigenicity in CSCs. Microarray analysis revealed that various cancer pathways were significantly activated following the suppression of . The changes seen might compensate for the loss of function, CSCs population's aggressiveness, which led to the CSCs population's aggressiveness. Thus, understanding the role of in regulating the stemness of CSCs may lead to targeted therapy for lung CSCs.
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http://dx.doi.org/10.7150/jca.50793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120186PMC
April 2021

Targeting Lung Cancer Stem Cells: Research and Clinical Impacts.

Front Oncol 2017 5;7:80. Epub 2017 May 5.

Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia.

Lung cancer is the most common cancer worldwide, accounting for 1.8 million new cases and 1.6 million deaths in 2012. Non-small cell lung cancer (NSCLC), which is one of two types of lung cancer, accounts for 85-90% of all lung cancers. Despite advances in therapy, lung cancer still remains a leading cause of death. Cancer relapse and dissemination after treatment indicates the existence of a niche of cancer cells that are not fully eradicated by current therapies. These chemoresistant populations of cancer cells are called cancer stem cells (CSCs) because they possess the self-renewal and differentiation capabilities similar to those of normal stem cells. Targeting the niche of CSCs in combination with chemotherapy might provide a promising strategy to eradicate these cells. Thus, understanding the characteristics of CSCs has become a focus of studies of NSCLC therapies.
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http://dx.doi.org/10.3389/fonc.2017.00080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5418222PMC
May 2017

Variant Alleles in XRCC1 Arg194Trp and Arg399Gln Polymorphisms Increase Risk of Gastrointestinal Cancer in Sabah, North Borneo.

Asian Pac J Cancer Prev 2016 ;17(4):1925-31

Biotechnology Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Malaysia E-mail :

Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We accordingly investigated the XRCC1 Arg194Trp and Arg399Gln SNPs in terms of GIC risk in Sabah.

Materials And Methods: We performed genotyping for both SNPs for 250 GIC patients and 572 healthy volunteers using a polymerase chain reaction- restriction fragment length polymorphism approach. We validated heterozygosity and homozygosity for both SNPs using direct sequencing.

Results: The presence of a variant 194Trp allele in the Arg194Trp SNP was significantly associated with a higher risk of GIC, especially with gastric and colorectal cancers. We additionally found that the variant 399Gln allele in Arg399Gln SNP was associated with a greater risk of developing gastric cancer. Our combined analysis revealed that inheritance of variant alleles in both SNPs increased the GIC risk in Sabah population. Based on our etiological analysis, we found that subjects ≥50 years and males who carrying the variant 194Trp allele, and Bajau subjects carrying the 399Gln allele had a significantly increased risk of GIC.

Conclusions: Our findings suggest that inheritance of variant alleles in XRCC1 Arg194Trp and Arg399Gln SNPs may act as biomarkers for the early detection of GIC, especially for gastric and colorectal cancers in the Sabah population.
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http://dx.doi.org/10.7314/apjcp.2016.17.4.1925DOI Listing
January 2017

Isolation and Characterization of Cancer Stem Cells of the Non-Small-Cell Lung Cancer (A549) Cell Line.

Methods Mol Biol 2016 ;1516:371-388

Regenerative Medicine Cluster, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, Penang, 13200, Malaysia.

Cancer is a major health problem worldwide. The failure of current treatments to completely eradicate cancer cells often leads to cancer recurrence and dissemination. Studies have suggested that tumor growth and spread are driven by a minority of cancer cells that exhibit characteristics similar to those of normal stem cells, thus these cells are called cancer stem cells (CSCs). CSCs are believed to play an important role in initiating and promoting cancer. CSCs are resistant to currently available cancer therapies, and understanding the mechanisms that control the growth of CSCs might have great implications for cancer therapy. Cancer cells are consist of heterogeneous population of cells, thus methods of identification, isolation, and characterisation of CSCs are fundamental to obtain a pure CSC populations. Therefore, this chapter describes in detail a method for isolating and characterizing a pure population of CSCs from heterogeneous population of cancer cells and CSCs based on specific cell surface markers.
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http://dx.doi.org/10.1007/7651_2016_326DOI Listing
January 2018
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