Publications by authors named "Nitish Bansal"

8 Publications

  • Page 1 of 1

A case report of drone injury and its relevance in India.

J Clin Orthop Trauma 2021 Aug 25;19:183-186. Epub 2021 May 25.

Department of Orthopaedics, MMUHSMC, Solan, Himachal Pradesh, India.

Drones are being used globally for varied purposes, including recreational, surveillance, and military. This ever-expanding usage has led to an increase in drone-related injuries. Blunt trauma to the head, eye globe injuries, and skin laceration being the commonest. We present a case of drone injury to the hands caused by its propeller blades.
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http://dx.doi.org/10.1016/j.jcot.2021.05.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178114PMC
August 2021

Spontaneous subcapital femoral neck fracture complicating osteonecrosis of femoral head.

Acta Orthop Belg 2021 Mar;87(1):25-34

Spontaneous subcapital fracture (SSF) of femoral neck in pre-existent osteonecrosis of femoral head (ONFH) is a rare presentation. Only a few cases have been reported so far and majority of them have been reported to have unilateral hip involvement. We retrospectively reviewed clinical-radiological data of 10 patients (12 hips) with SSF complicating ONFH. All of them underwent uncemented total hip arthroplasty. All the available publications in the English language based medical literature were critically reviewed and results were summarized. The median age of presentation was 32 years (range : 24 years to 61 years). They were followed up for a mean duration of 25 months (range : 12 months to 59 months). The most common risk factor was corticosteroid consumption (7 out of 10 patients). All except one (modified Ficat and Arlet stage II) belonged to advanced stage of ONFH {stage III 3 patients (3 hips), stage IV 6 patients (8 hips)}. The mean time lag of ONFH to presentation was 22.3 months (range : 5 months to 60 months), and SSF to presentation was 13.8 days (range : 1 day to 28 days). Mean pre- operative Harris Hip Score was 10.8 (range : 8 to 14), which improved to 93 (range : 91 to 96) after total hip arthroplasty when last followed up (p<0.05). Corticosteroids induced ONFH has a propensity to develop SSF. This entity should find a place in existing classification system.
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March 2021

Dataset of next-generation sequence reads of nanobody clones in phage display library derived from Indian desert camel ( L.).

Data Brief 2021 Feb 16;34:106663. Epub 2020 Dec 16.

Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India.

Next-generation sequences (NGS) dataset of nanobody (Nb) clones in a phage display library (PDL) is of immense value as it serves in many different ways, such as: i). estimating the library size, ii). improving selection and identification of Nbs, iii). informing about frequency of V gene families, diversity and length of CDRs, iv). high resolution analysis of natural and synthetic libraries, etc. [1], [2], [3]. We used a fraction of our previously constructed PDL of Nbs derived from an lipopolysaccharide-immunized Indian desert camel in order to obtain the dataset of NGS reads of Nbs. The cryo-preserved transformants library was revived to extract the Nb-encoding VHH (inserts)-pHEN4 (vector) DNA pool. The DNA sample was used for amplifying VHH pool by PCR [6]. The VHH amplicons band was gel-purified and subjected to NGS using Illumina MiSeq platform. 'Nextra XT micro V2 Index' kit was used for the Nb library DNA sample sequencing, with the adaptors: 'i7' (N706: TAGGCATG) and 'i5' (S517: GCGTAAGA). The raw data comprised of a total read count of 182146 (matched= 179591; unmatched=2555), with average read length of 130.33 bases and a total of 23.74 Mb. Of 179591 matched reads, 142004 were paired reads and 37587 broken paired reads. The raw data of NGS reads was submitted to NCBI Sequence Reads Archive accessible at URL: https://www.ncbi.nlm.nih.gov/Traces/study/?acc=PRJNA516512 (dataset ref. [7]), and after analysis deposited in Mendeley Datasets repository, which is accessible at URL: [https://data.mendeley.com/datasets/4rsz3snvk5/3] (dataset ref. [8]). The sequence reads were analyzed by bioinformatics tools [9], [10], [11], [12]. The assembled consensus contigs revealed Nb orthologs of diverse Ag-specificities, including those isolated by conventional panning and Sanger-sequenced functional Nbs. Contig 1 CDR1-3 matched to those of anti- RoTat1.2 variant surface glycoprotein (VSG), while Contig 2 CDR1-3 matched to those of anti-LPS Nb clones isolated from the library. Contig 3 was however incomplete and lacked CDR3. Despite lacking the depth, the NGS data is a useful guide for selection of antigen-specific Nbs from the library, as demonstrated by anti- VSG Nbs, and provides templates for Nb-based diagnostic reagents and therapeutic agents.
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http://dx.doi.org/10.1016/j.dib.2020.106663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770539PMC
February 2021

Bilateral Asymmetric Hip Dislocations with Vascular Compromise: A Case Report.

Indian J Orthop 2020 Dec 15;54(Suppl 2):416-420. Epub 2020 Sep 15.

Department of Orthopaedics, GMC, Patiala, Punjab India.

Bilateral asymmetric hip dislocations are rare injuries and associated vascular compromise is even rarer. We report a unique case of bilateral asymmetric hip dislocations with vascular compromise, due to mechanical arterial compression by the anteriorly dislocated femoral head on one side. Prompt closed reduction re-established the blood flow. Operative intervention was done subsequently to remove intra-articular loose bodies in the hip on the same side. At 6-month follow-up, patient had good functional outcome and no radiographic evidence of complications.
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http://dx.doi.org/10.1007/s43465-020-00255-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609650PMC
December 2020

Assessment of Bone Quality Using Radiogrammetric Parameters of Proximal Humerus in India: Defining the Osteoporotic Fracture Risk Limit Value and its Reliability.

Indian J Orthop 2020 Dec 9;54(Suppl 2):307-315. Epub 2020 Aug 9.

Department of Orthopaedic Surgery, Maulana Azad Medical College and Associated Lok Nayak Hospital, c/o Mr Raj Kumar Arora, B-253, Second floor, New Delhi, 110002 India.

Background: World health organization (WHO) has defined osteoporosis clinically on the basis of bone mineral density (BMD) measurement by dual-energy X-ray absorptiometry (DXA) scan and the presence of fractures. This facility is expensive and not readily available in majority of the centers in India. The authors have attempted to study defined measurements on radiographs (radiogrammetric parameters) to diagnose osteoporosis in Indian population.

Patients And Methods: We prospectively studied 200 proximal humerus radiographs for measuring radiogrammetric parameters and divided into Group A and B ( = 100 in each group). Group A involved patients with age < 50 years and without any illness affecting bone quality. Group B involved patients with age > 50 years and sustained acute osteoporotic fractures of distal radius/ anterior wedge vertebral fracture/intertrochanteric fracture following trivial trauma. Three parameters (cortical thickness, cortical index and deltoid tuberosity index) were measured by 3 observers at 2 different occasions.

Results: The mean age of patients was 37.87 years and 58.38 years for group A and B, respectively. The 'cortical thickness' of the proximal humerus diaphysis had the mean value for Group A and B to be 0.4 ± 0.07 cm and 0.33 ± 0.06 cm respectively. The mean values for the 'cortical index' of proximal humerus came out to be 0.4 ± 0.07 for group A and 0.32 ± 0.06 for group B. The 'deltoid tuberosity index' measurements showed the mean values for group A and B were 1.81 ± 0.23 and 1.55 ± 0.16, respectively. Inter-observer reliability for single measures was excellent for deltoid tuberosity index (ICC 0.8077) and good for cortical thickness (ICC 0.7032) and cortical index (ICC 0.7357). Observer 1 had excellent intra-observer reliabilities for all the three parameters. Observer 2 and 3 had excellent reliability for deltoid tuberosity index and good intra-observer reliability for cortical thickness and cortical index. The cortical thickness had a cut off of ≤ 0.372 cm with a sensitivity of 86.02 and specificity of 82.12. The cortical index had a cut off of ≤ 0.378 with a sensitivity of 89.16 and specificity of 84.22. The deltoid tuberosity index had a cut off of ≤ 1.684 with a sensitivity of 96.61 and specificity of 84.08.

Conclusion: The outcome of this study is likely to help in early diagnosis of osteoporosis at the community level in the absence of DXA scan as it identifies threshold values for radiogrammetric parameters which can be a predictor of the osteoporosis. The deltoid tuberosity index was found to be the most suitable of these parameters.
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http://dx.doi.org/10.1007/s43465-020-00224-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609647PMC
December 2020

Reconsidering as a proxy for zoonotic tuberculosis: a molecular epidemiological surveillance study.

Lancet Microbe 2020 Jun;1(2):e66-e73

Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada.

Background: Zoonotic tuberculosis is defined as human infection with . Although globally, India has the largest number of human tuberculosis cases and the largest cattle population, in which bovine tuberculosis is endemic, the burden of zoonotic tuberculosis is unknown. The aim of this study was to obtain estimates of the human prevalence of animal-associated members of the complex (MTBC) at a large referral hospital in India.

Methods: We did a molecular epidemiological surveillance study of 940 positive mycobacteria growth indicator tube (MGIT) cultures, collected from patients visiting the outpatient department at Christian Medical College (Vellore, India) with suspected tuberculosis between Oct 1, 2018, and March 31, 2019. A PCR-based approach was applied to subspeciate cultures. Isolates identified as MTBC other than or as inconclusive on PCR were subject to whole-genome sequencing (WGS), and phylogenetically compared with publicly available MTBC sequences from south Asia. Sequences from WGS were deposited in the National Center for Biotechnology Information Sequence Read Archive, accession number SRP226525 (BioProject database number PRJNA575883).

Findings: The 940 MGIT cultures were from 548 pulmonary and 392 extrapulmonary samples. A conclusive identification was obtained for all 940 isolates; wild-type was not identified. The isolates consisted of (913 [97·1%] isolates), (seven [0·7%]), BCG (five [0·5%]), and non-tuberculous mycobacteria (15 [1·6%]). Subspecies were assigned for 25 isolates by WGS, which were analysed against 715 MTBC sequences from south Asia. Among the 715 genomes, no was identified. Four isolates of cattle origin were dispersed among human sequences within lineage 1, and the seven isolates from human MGIT cultures were dispersed among sequences from cattle.

Interpretation: prevalence in humans is an inadequate proxy of zoonotic tuberculosis. The recovery of from humans highlights the need to use a broadened definition, including MTBC subspecies such as , to investigate zoonotic tuberculosis. The identification of in cattle also reinforces the need for One Health investigations in countries with endemic bovine tuberculosis.

Funding: Bill & Melinda Gates Foundation, Canadian Institutes for Health Research.
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http://dx.doi.org/10.1016/S2666-5247(20)30038-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325494PMC
June 2020

A comprehensive study on seroprevalence of bluetongue virus in Haryana state of India.

Vet World 2017 Dec 13;10(12):1464-1470. Epub 2017 Dec 13.

Department of Animal Nutrition, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar - 125 004, Haryana, India.

Aim: The aim of present study was to determine seroprevalence of bluetongue virus (BTV) in Haryana state of India.

Materials And Methods: A total of 803 serum samples, 408 of cattle and 395 of buffalo origin, respectively, were collected from different villages of Haryana. Sampling was done randomly to obtain unbiased results. The samples were evaluated by a competitive enzyme-linked immunosorbent assay for the presence of BTV antibodies.

Results: Overall seroprevalence of BTV antibody in cattle and buffaloes for all 21 districts of Haryana state was found to be 75.49% and 92.91%, respectively. The prevalence of BTV in different agroclimatic zones ranged between 72-77% and 90-94% for cattle and buffalo, respectively. In buffaloes, the BTV seroprevalence was comparatively higher than in cattle.

Conclusion: The study showed that BTV is circulating in cattle and buffalo populations in the Northern part of India.
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http://dx.doi.org/10.14202/vetworld.2017.1464-1470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771171PMC
December 2017

Molecular analysis of genome segment-3 of bluetongue virus serotype 12 isolates from Haryana.

Vet World 2017 Nov 27;10(11):1389-1393. Epub 2017 Nov 27.

Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar - 125 004, Haryana, India.

Aim: The present study was designed to characterize the genome segment 3 (Seg-3) of bluetongue virus (BTV) serotype 12 isolates from different outbreaks of Bluetongue disease in Haryana, India.

Materials And Methods: Blood and swab samples were collected from goat and sheep suspected to be suffering of BT from different outbreaks from Gurugram, Sirsa, Hisar, and Karnal districts of Haryana. The samples were grown in insect and mammalian cell lines. After preliminary identification, serotyping was done using BTV type-specific quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Sequencing was performed using terminal and walking internal primers specific for Seg-3 on ABI Capillary Sequencer 3130 using a "BigDye cycle sequencing kit." The obtained sequence data were analyzed with various bioinformatic tools.

Results: Real-time PCR results confirmed the samples to be positive for BTV-12. The Seg-3 of Indian isolates was most closely related to that of a south Indian isolate of BTV-12 from Andhra Pradesh (KC662614) with 97% nucleotide identity.

Conclusions: The study confirmed the circulation of BTV-12 in Haryana, India. The variations shown in genome Seg-3 of BTV-12 isolates may have some significance and need to be further explored.
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http://dx.doi.org/10.14202/vetworld.2017.1389-1393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732348PMC
November 2017
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