Publications by authors named "Nitesh Malhotra"

12 Publications

  • Page 1 of 1

Point of care detection of COVID-19: Advancement in biosensing and diagnostic methods.

Chem Eng J 2021 Jun 31;414:128759. Epub 2021 Jan 31.

Department of Chemistry, Karnatak University, Dharwad 580 007, India.

The recent outbreak of COVID-19 has created much inconvenience and fear that the virus can seriously affect humans, causing health hazards and death. This pandemic has created much worry and as per the report by World Health Organization (WHO), more than 43 million individuals in 215 countries and territories were affected. People around the world are still struggling to overcome the problems associated with this pandemic. Of all the available methods, reverse-transcriptase polymerase chain reaction (RT-PCR) has been widely practiced for the pandemic detection even though several diagnostic tools are available having varying accuracy and sensitivity. The method offers many advantages making it a life-saving tool, but the method has the limitation of transporting to the nearest pathology lab, thus limiting its application in resource limited settings. This has a risen a crucial need for point-of-care devices for on-site detection. In this venture, biosensors have been used, since they can be applied immediately at the point-of-care. This review will discuss about the available diagnostic methods and biosensors for COVID-19 detection.
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http://dx.doi.org/10.1016/j.cej.2021.128759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847737PMC
June 2021

Prussian blue nanocubes/carbon nanospheres heterostructure composite for biosensing of metformin.

Int J Nanomedicine 2018 15;13(T-NANO 2014 Abstracts):117-120. Epub 2018 Mar 15.

Department of Biochemistry, M. D. University, Rohtak, Haryana, India.

This paper reports the fabrication of highly sensitive metformin sensor based on Prussian blue (PB) nanocubes/carbon nanosphere (CNS) heterostructures composed of a perfect cube and spherical composite on a fluorine-doped tin oxide surface. Due to the excellent biocompatibility of PB nanocubes the PB/CNS based Mf sensor exhibited a wide linear range of 0.001-10 mM with a response duration of less than 5 s and a detection limit (based on signal to noise ratio) of 0.1 µM.
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http://dx.doi.org/10.2147/IJN.S125153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419316PMC
April 2019

Correction to: Voltammetric detection of anti-HIV replication drug based on novel nanocomposite gold-nanoparticle-CaCO hybrid material.

Bioprocess Biosyst Eng 2019 06;42(6):1077-1079

Department of Biochemistry, M. D. University, Rohtak, 124001, Haryana, India.

The Figs. 2, 4 and 5 were published wrongly in this paper, due to inadvertent compilation of figures during uploading the paper.
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http://dx.doi.org/10.1007/s00449-019-02097-7DOI Listing
June 2019

Detection of alprazolam with a lab on paper economical device integrated with urchin like [email protected] Pd shell nano-hybrids.

Mater Sci Eng C Mater Biol Appl 2017 Nov 5;80:728-735. Epub 2016 Dec 5.

Department of Biochemistry, MD University, Rohtak -124001, Haryana, India.

We present results of the studies relating to fabrication of a microfluidic biosensor chip based on urchin like [email protected] Pd shell nano-hybrids that is capable of sensing alprazolam through electrochemical detection. Using this chip we demonstrate, with high reliability and in a time efficient manner, the detection of alprazolam present in buffer solutions at clinically relevant concentrations. Methylene blue (MB) was also doped as redox transition substance for sensing alprazolam. Nano-hybrids modified EμPAD showed wide linear range 1-300ng/ml and low detection limit of 0.025ng/l. Low detection limit can further enhance its suitability for forensic application. Nano-hybrids modified EμPAD was also employed for determination of drug in real samples such as human urine. Reported facile lab paper approach integrated with urchin like [email protected] Pd shell nano-hybrids could be well applied for the determination of serum metabolites.
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http://dx.doi.org/10.1016/j.msec.2016.11.128DOI Listing
November 2017

Graphene nanoflakes on transparent glass electrode sensor for electrochemical sensing of anti-diabetic drug.

Bioprocess Biosyst Eng 2017 Apr 19;40(4):537-548. Epub 2016 Dec 19.

Amity Institute of Nanotechnology (AINT), AMITY University, Noida, 201313, Uttar Pradesh, India.

Metformin (Mf) plays a major role in controlling insulin level of individuals at risk of developing diabetes mellitus. Overdose of Mf can cause lactic acidosis, diarrhoea, cough, or hoarseness, etc. These particulars point out the identification for selective and sensitive methods of Mf determination. In the present work, graphene nanoflakes-polymethylene blue (GNF-PMB) nano-composites were developed onto fluorine-doped tin oxide (SnO/F) coated glass substrates for electrochemical sensing of Mf using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The developed sensor shows quick response time (10 s), linearity as 10-10 µM, LOD (0.1 nM), and good shelf life (10 weeks). Attempts have been made to utilize this electrode for estimation of Mf in urine samples. Configured as a highly responsive, reproducible Mf sensor, it combines the electrical properties of GNF and stable electron transfer of PMB. The newly developed Mf sensor presents a promising candidate in point-of-care diagnosis.
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http://dx.doi.org/10.1007/s00449-016-1719-1DOI Listing
April 2017

An enzyme free Vitamin C augmented sensing with different ZnO morphologies on SnO2/F transparent glass electrode: A comparative study.

Mater Sci Eng C Mater Biol Appl 2016 Dec 7;69:769-79. Epub 2016 Jul 7.

Amity Institute of Nanotechnology, Amity University, Noida, UP, India. Electronic address:

Three types of Zinc oxide (ZnO) nanostructures viz. ZnO nanocrystals (ZnONCs), ZnO nanoparticles (ZnONPs) and ZnO nanobelts (ZnONBs) were synthesized and characterized by UV-Vis, FTIR and SEM. A comparison of signal amplification by these ZnO nanostructures as judged by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Linear Sweep Voltammetry (LSV) revealed that ZnONCs are better sensing interface for electrochemical detection. When these ZnO nanostructure were compared electrochemically for sensing Vitamin C, ZnONC's sensor outperformed the ZnONP and ZnONB sensor and previously reported sensors. The ZnONCs/MB/FTO electrode showed a wide linear sensing range (0.001μM to 4000μM), low detection limit (0.0001μM), a small response time (5s) and a storage stability of 6months. To the best of our knowledge, this elevated sensitivity and remarkable stability for electrochemical Vitamin C detection using ZnONC's have not been reported so far.
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http://dx.doi.org/10.1016/j.msec.2016.07.012DOI Listing
December 2016

Point of care with micro fluidic paper based device integrated with nano zeolite-graphene oxide nanoflakes for electrochemical sensing of ketamine.

Biosens Bioelectron 2017 Feb 17;88:249-257. Epub 2016 Aug 17.

Department of Biochemistry, MD University, Rohtak, Haryana, India.

The present study was aimed to develop an ultrasensitive technique for electroanalysis of ketamine; a date rape drug. It involved the fabrication of nano-hybrid based electrochemical micro fluidic paper-based analytical device (EμPADs) for electrochemical sensing of ketamine. A paper chip was developed using zeolites nanoflakes and graphene-oxide nanocrystals (Zeo-GO). EμPAD offers many advantages such as facile approach, economical and potential for commercialization. Nanocrystal modified EμPAD showed wide linear range 0.001-5nM/mL and a very low detection limit of 0.001nM/mL. The developed sensor was tested in real time samples like alcoholic and non-alcoholic drinks and found good correlation (99%). The hyphenation of EμPAD integrated with nanocrystalline Zeo-GO for detection of ketamine has immense prospective for field-testing platforms. An extensive development could be made for industrial translation of this fabricated device.
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http://dx.doi.org/10.1016/j.bios.2016.08.043DOI Listing
February 2017

Impedimetric genosensor for ultratrace detection of hepatitis B virus DNA in patient samples assisted by zeolites and MWCNT nano-composites.

Biosens Bioelectron 2016 Dec 7;86:566-574. Epub 2016 Jul 7.

Deptt. of Biochemistry, MD University, Rohtak, India.

Nanocrystals of zeolites (Nanocrys Zeo) and Multi-walled carbon nanotubes (MWCNT) based diagnostic genosensor was employed for detection of polymerase chain (PCR) amplified HBVDNA in blood of hepatitis B patients. The ssDNA-nanocomposite modified electrode was characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The hybridization between ss DNA probe and target ss DNA was detected by reduction in current, generated by interaction of methylene blue (MB) with free guanine (3'G) of ssDNA. Nanocrys zeo were deposited on the Fluorine doped tin oxide glass electrode (FTO) by drop-casting method for better immobilization of ss DNA while MWCNTs are incorporated into the zeolite-assembly to enhance the electro-conductivity of the present genosensor. The ssDNA-nanocomposite modified FTO electrode exhibited optimum current within 5s, at pH 5.6, and incubation temperature of 45°C. The value of charge transfer resistance (Rct) was linear with the number of copies of target DNA between 150 and 10(6) copies/ml. The limit of detection (LOD) of the sensor was 50 copies/ml. Within and between batches coefficients of variation (CV) were 2.5% and 3.2% respectively. Results obtained with our genosensor were also correlated with those by RT-PCR and r(2) value found with good accuracy of 97%. The electrode was reused by dipping it into 0.1M NaOH for 3min and lost 50% of its initial activity in 4 weeks. Furthermore the technique employed for detection of HBV is EIS, which is convenient and required less analysis time.
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http://dx.doi.org/10.1016/j.bios.2016.07.013DOI Listing
December 2016

Hierarchical electrodeposition of methylene blue on ZnO nanocrystals thin films layered on SnO2/F electrode for in vitro sensing of anti-thalassemic drug.

Mater Sci Eng C Mater Biol Appl 2016 May;62:596-604

Amity Institute of Nanotechnology, Amity University, Noida, UP, India. Electronic address:

Zinc oxide nanocrystals-methylene blue nanocomposites were developed by electrodeposition of methylene blue onto the thin films of zinc oxide nanocrystals deposited onto SnO2/F coated glass substrates for in vitro sensing of anti-thalassemic drug i.e. deferiprone. Detailed morphological, electrochemical, structural and optical characterizations of ZnONC-MB/FTO electrode were done using XRD, SEM, EIS, FTIR, LSV, and CV and show quick response time (within 5 s), linearity as 1 × 10(-3) to 10(3) μM and shelf life of about 10 weeks under refrigerated conditions. Attempts have been made to utilize this electrode for estimation of deferiprone in urine samples. The developed sensor exhibited high reproducibility and good storage stability.
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http://dx.doi.org/10.1016/j.msec.2016.02.006DOI Listing
May 2016

Nanocrystals of zeolite act as enhanced sensing interface for biosensing of leviteracetum.

J Pharm Sci 2015 Mar 1;104(3):1153-9. Epub 2014 Dec 1.

Amity Institute of Nanotechnology, AMITY University, Noida, UP, India.

In present work, nanocrystals of zeolites were employed as sensing interface for augmented electrochemical sensing of antiepileptic drug, that is, leviteracetum (LEV). Electrochemical sensing method was developed by depositing nanocrystals of zeolites and horseradish peroxidase (nanocrys zeolites-HRP) onto indium tin oxide (ITO)-coated glass surface for LEV detection. Various stages of biosensor fabrication were characterized by Transmission electron microscopy, X-ray diffraction, scanning electron microscopy, and electrochemical impedance spectroscopy. The fabricated nanocrys zeolites-HRP-modified sensor exhibited wide linear range (10-500 μM) and a low detection limit of 0.01 μM. The biosensor also showed a short response time (within 2 s). In addition, the biosensor exhibited high reproducibility, good storage stability, and anti-interference ability. The applicability of the nanocrys zeolites-HRP-modified sensor is to determine LEV level in spiked serum samples.
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http://dx.doi.org/10.1002/jps.24267DOI Listing
March 2015

Electrochemical impediometric detection of anti-HIV drug taking gold nanorods as a sensing interface.

Biosens Bioelectron 2015 Apr 24;66:332-7. Epub 2014 Nov 24.

Department of Biochemistry, M. D. University, Rohtak 124001, Haryana, India.

In present work, gold nanorods were used for amplification of electrochemical sensing of anti-HIV replication drug i.e. deferiprone. Gold nanorods (nano Au) deposited onto pencil graphite electrode (PGE) has been utilized for covalent immobilization of horse radish peroxidase (HRP), via glutaraldehyde (Glu), for deferiprone detection using impedimetric technique. Gold nanorods (nano Au) prepared were characterized by TEM and XRD. The resulting nano Au sensor exhibited a good response to deferiprone with a wide linear range (0.005-1000µM) and a low detection limit 0.005µM. The biosensor also showed a short response time (within 15s). In addition, the biosensor exhibited high reproducibility, good storage stability and anti-interference ability. The applicability of the nano Au sensor is to determine deferiprone level in spiked urine and serum samples.
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http://dx.doi.org/10.1016/j.bios.2014.11.038DOI Listing
April 2015

Voltammetric detection of anti-HIV replication drug based on novel nanocomposite gold-nanoparticle-CaCO3 hybrid material.

Bioprocess Biosyst Eng 2015 May 22;38(5):815-22. Epub 2014 Nov 22.

Amity Institute of Nanotechnology, AMITY University, Noida, UP, India,

A novel bionanocomposite, horse radish peroxidase- gold-nanoparticle-Calcium carbonate (HRP-AuNPs-CaCO3), hybrid material was encapsulated by silica sol on a glassy carbon electrode (GCE). The fabricated modified electrode was used as a novel voltammetric sensor for electrochemical sensing of anti-HIV replication drug i.e. deferiprone. The surface morphology of the modified electrode was characterized by scanning electron microscopy (SEM). Results obtained from the voltammetric measurements show that HRP-AuNPs-CaCO3 modified GCE offers a selective and sensitive electrochemical sensor for the determination of deferiprone. Under experimental conditions, the proposed voltammetric sensor has a linear response range from 0.01 to 10,000 μM with a detection limit of 0.01 μM. Furthermore, the fabricated sensor was successfully applied to determine deferiprone level in spiked urine and serum samples.
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http://dx.doi.org/10.1007/s00449-014-1323-1DOI Listing
May 2015
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