Publications by authors named "Ning Zhao"

721 Publications

Alkaline Phosphatase Electrochemical Micro-Sensor Based on 3D Graphene Networks for the Monitoring of Osteoblast Activity.

Biosensors (Basel) 2022 Jun 13;12(6). Epub 2022 Jun 13.

State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

Alkaline phosphatase (ALP) is a significant biomarker that indicates osteoblast activity and skeletal growth. Efficient ALP detection methods are essential in drug development and clinical diagnosis. In this work, we developed an in-situ synthesized three-dimensional graphene networks (3DGNs)-based electrochemical sensor to determine ALP activity. The sensor employs an ALP enzymatic conversion of non-electroactive substrate to electroactive product and presents the ALP activity as an electrochemical signal. With 3DGNs as the catalyst and signal amplifier, a sample consumption of 5 μL and an incubation time of 2 min are enough for the sensor to detect a wide ALP activity range from 10 to 10,000 U/L, with a limit of detection of 5.70 U/L. This facile fabricated sensor provides a quick response, cost-effective and non-destructive approach for monitoring living adherent osteoblast cell activity and holds promise for ALP quantification in other biological systems and clinical samples.
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http://dx.doi.org/10.3390/bios12060406DOI Listing
June 2022

Activation of transient receptor potential vanilloid 4 is involved in pressure overload-induced cardiac hypertrophy.

Elife 2022 Jun 22;11. Epub 2022 Jun 22.

Department of Cardiology, Xuzhou Central Hospital, Xuzhou, China.

Previous studies, including our own, have demonstrated that transient receptor potential vanilloid 4 (TRPV4) is expressed in hearts and implicated in cardiac remodeling and dysfunction. However, the effects of TRPV4 on pressure overload-induced cardiac hypertrophy remain unclear. In this study, we found that TRPV4 expression was significantly increased in mouse hypertrophic hearts, human failing hearts, and neurohormone-induced hypertrophic cardiomyocytes. Deletion of TRPV4 attenuated transverse aortic constriction (TAC)-induced cardiac hypertrophy, cardiac dysfunction, fibrosis, inflammation, and the activation of NFκB - NOD - like receptor pyrin domain-containing protein 3 (NLRP3) in mice. Furthermore, the TRPV4 antagonist GSK2193874 (GSK3874) inhibited cardiac remodeling and dysfunction induced by TAC. In vitro, pretreatment with GSK3874 reduced the neurohormone-induced cardiomyocyte hypertrophy and intracellular Ca concentration elevation. The specific TRPV4 agonist GSK1016790A (GSK790A) triggered Ca influx and evoked the phosphorylation of Ca/calmodulin-dependent protein kinase II (CaMKII). But these effects were abolished by removing extracellular Ca or GSK3874. More importantly, TAC or neurohormone stimulation-induced CaMKII phosphorylation was significantly blocked by TRPV4 inhibition. Finally, we show that CaMKII inhibition significantly prevented the phosphorylation of NFκB induced by GSK790A. Our results suggest that TRPV4 activation contributes to pressure overload-induced cardiac hypertrophy and dysfunction. This effect is associated with upregulated Ca/CaMKII mediated activation of NFκB-NLRP3. Thus, TRPV4 may represent a potential therapeutic drug target for cardiac hypertrophy and dysfunction after pressure overload.
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http://dx.doi.org/10.7554/eLife.74519DOI Listing
June 2022

Bidirectional influences between seizures and dementia: A systematic review and meta-analysis.

Int J Geriatr Psychiatry 2022 Jul;37(7)

Shanghai Garrison Hongkou Third Retired Cadres Rest House, Shanghai, China.

Objectives: Dementia and seizures often co-exist, but the association between these two disorders is not well established. Therefore, this systematic review and meta-analysis aimed to clarify the relationship between seizures and dementia.

Methods: The PubMed, EMBASE, CBM, and CNKI databases were used to search for relevant publications from inception to August 25, 2021. Data extraction was performed by two authors independently. The random-effects model was adopted to evaluate the pooled estimates.

Results: Two nested case-control studies and 18 cohort studies were included in the meta-analysis. Seizures were associated with the development of dementia and Alzheimer's disease (AD), and the pooled relative risk (RR) was 2.51 [95% confidence intervals (CI) = 1.87-3.36, p < 0.001] and 1.61 (95% CI = 1.42-1.82, p < 0.001), respectively. Pooled RR estimating the effect of dementia on seizures risk was 3.68 (95% CI = 3.05-4.44, p < 0.001). In addition, the pooled effect size of dementia on epilepsy risk was 3.02 (95% CI = 2.16-4.23, p < 0.001). The subgroup analyses suggested that vascular risk factors could confound the associations between these two disorders. Seizures might independently and significantly increase the risk of dementia, and in turn, dementia might predispose an individual to a higher risk of seizures.

Conclusions: These results suggested that dementia and seizures share common pathogenesis and might be treated with similar preventive treatment measures. Vascular changes in patients with dementia or seizures should also be examined.
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http://dx.doi.org/10.1002/gps.5723DOI Listing
July 2022

Pan-Cancer Methylated Dysregulation of Long Non-coding RNAs Reveals Epigenetic Biomarkers.

Front Cell Dev Biol 2022 27;10:882698. Epub 2022 May 27.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

Different cancer types not only have common characteristics but also have their own characteristics respectively. The mechanism of these specific and common characteristics is still unclear. Pan-cancer analysis can help understand the similarities and differences among cancer types by systematically describing different patterns in cancers and identifying cancer-specific and cancer-common molecular biomarkers. While long non-coding RNAs (lncRNAs) are key cancer modulators, there is still a lack of pan-cancer analysis for lncRNA methylation dysregulation. In this study, we integrated lncRNA methylation, lncRNA expression and mRNA expression data to illuminate specific and common lncRNA methylation patterns in 23 cancer types. Then, we screened aberrantly methylated lncRNAs that negatively regulated lncRNA expression and mapped them to the ceRNA relationship for further validation. 29 lncRNAs were identified as diagnostic biomarkers for their corresponding cancer types, with lncRNA was identified as a new KIRC-associated biomarker, and lncRNA was regarded as a carcinogenic factor of KIRP. Two lncRNAs and were identified as pan-cancer biomarkers. In general, the cancer-specific and cancer-common lncRNA biomarkers identified in this study may aid in cancer diagnosis and treatment.
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http://dx.doi.org/10.3389/fcell.2022.882698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200062PMC
May 2022

Resistance to ALS inhibitors conferred by non-target-site resistance mechanisms in Myosoton aquaticum L.

Pestic Biochem Physiol 2022 Jun 5;184:105067. Epub 2022 Mar 5.

College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, PR China. Electronic address:

Myosoton aquaticum L. is a competitive broadleaf weed commonly found in wheat fields in China and has become challenging due to its evolving herbicide resistance. In this study, one subpopulation, RF1 (derived from the tribenuron-methyl-resistant population HN10), with none of the known acetolactate synthase (ALS) resistance mutations was confirmed to exhibit resistance to tribenuron-methyl (SU), pyrithiobac‑sodium (PTB), florasulam (TP), flucarbazone-Na (SCT), and diflufenican (PDS). In vitro ALS activity assays showed that the total ALS activity of RF1 was lower than that of the susceptible (S) population. However, there was no difference in ALS gene expression induced by tribenuron-methyl between the two populations. The combination of the cytochrome P450 monooxygenase (P450) inhibitor malathion and tribenuron-methyl resulted in the RF1 population behaving like the S population. The rapid P450-mediated tribenuron-methyl metabolism in RF1 plants was also confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. In addition, approximately equal glutathione S-transferase (GST) activity was observed in RF1 and S plants of untreated and tribenuron-methyl treated groups. This study reported one M. aquaticum L. population without ALS resistance mutations exhibiting resistance to ALS inhibitors and the PDS inhibitor diflufenican, and the non-target-site resistance mechanism played a vital role in herbicide resistance.
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http://dx.doi.org/10.1016/j.pestbp.2022.105067DOI Listing
June 2022

Serum metabolomics analysis of deficiency pattern and excess pattern in patients with rheumatoid arthritis.

Chin Med 2022 Jun 15;17(1):71. Epub 2022 Jun 15.

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Dongcheng District, Beijing, 100700, China.

Background: Rheumatoid arthritis (RA) is a chronic and refractory autoimmune disease. Deficiency pattern (DP) and excess pattern (EP), as crucial types of Chinese medicine pattern diagnoses published by International Classification of Diseases 11th Revision (ICD-11), could provide new strategies for RA diagnosis. However, the biological basis of DP and EP of RA is not explicit.

Methods: 19 female RA DP patients, 41 female RA EP patients and 30 female healthy participants were included in the study. The serums of participants were collected and analyzed by metabolomics based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to profile metabolic characteristics of RA DP and EP. Furthermore, bioinformatics analysis results were obtained by using Ingenuity Pathway Analysis (IPA) and statistical analysis was performed by SAS version 9.4 for further identification of potential biomarkers.

Results: Serum metabolic profiling revealed 25 and 24 differential metabolites in RA DP and EP respectively, and 19 metabolites were common to RA DP and EP. Compared with DP group, L-Homocysteic acid, LysoPE(P-16:0/0:0), N(omega)-Hydroxyarginine and LysoPC(16:0/0:0) decreased (P < 0.05), and Pyruvic acid, D-Ribose, Gamma-Glutamylserine, PE(22:0/24:1(15Z)), Inosinic acid increased (P < 0.05) in EP group. Menawhile, S-Nitrosoglutathione, 5-Thymidylic acid, SN38 glucuronide, PE(22:0/24:0), PC(24:0/24:1(15Z)) and Bisdiphosphoinositol tetrakisphosphate increased significantly in DP group compared to EP group (P < 0.05). For the unique metabolites, bioinformatics analysis results showed that 5-Methoxytryptamine involved in Melatonin Degradation II and Superpathway of Melatonin Degradation is the key metabolite to RA DP. Meanwhile, GABA is the key metabolite in EP group, which involved in Glutamate Dependent Acid Resistance, GABA Receptor Signaling, Glutamate Degradation III (via 4-aminobutyrate) and 4-aminobutyrate Degradation I. Bioinformatics analysis between unique metabolites of RA DP and EP groups with human target genes for RA showed that 5-methoxytryptamine and LysoPC(18:1(9Z)/0:0), the unique metabolites of RA DP, might participate in colorectal cancer metastasis signaling, tumor microenvironment pathway, apoptosis signaling, MYC mediated apoptosis signaling, erythropoietin signaling pathway and LXR/RXR activation. Simultaneously, GABA, LysoPA(18:1(9Z)/0:0) and L-Targinine, the unique metabolites of RA EP, might participate in neuroinflammation signaling pathway, osteoarthritis pathway, glucocorticoid receptor signaling, ILK signaling, IL-17 signaling and HIF1α signaling.

Conclusions: The study indicates that serum metabolomics preliminarily revealed the biological basis of RA DP and EP. 5-methoxytryptamine, LysoPC(18:1(9Z)/0:0) and GABA, LysoPA(18:1(9Z)/0:0), L-Targinine might be the predictors to distinguish the DP and EP of RA respectively. These interesting results provide thoughts for further study of traditional medicine patterns of ICD-11. It also contributes to provide strategy for personalized precision treatment of RA and further validation is needed.
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http://dx.doi.org/10.1186/s13020-022-00632-5DOI Listing
June 2022

Quantitative x-ray fluorescence imaging system for non-destructive 3D tumor histology.

Opt Lett 2022 Jun;47(12):3087-3090

An in-house dual-modality x-ray fluorescence tomography (XFT) and x-ray computed tomography (XCT) system was developed to quantify iodine contrast distribution through the whole tumor volume ex vivo. The quantitative XFT was calibrated with water phantoms containing iodine solutions of various concentrations (0.0175-1.4 wt.%). The vasculature distribution was reflected by the iodine perfusion, which was validated with histology. This technique may open a new, to the best of our knowledge, route to the non-destructive three-dimensional-imaging-based histological analysis of tumor samples.
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http://dx.doi.org/10.1364/OL.458905DOI Listing
June 2022

The Role of ANGPTL Gene Family Members in Hepatocellular Carcinoma.

Dis Markers 2022 1;2022:1844352. Epub 2022 Jun 1.

Department of Oncology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Hepatocellular carcinoma (HCC) is highly aggressive with a poor prognosis and survival rate. Certain ANGPTL members have been implicated in tumor progression. However, the relevance of the ANGPTL gene family to HCC remains poorly understood. In this study, we explored the role of ANGPTLs in the prognosis of HCC.

Methods: From the CCLE database, we studied the expression of ANGPTLs in a range of cancer cell lines. The UCSC, HCCDB, and Human Protein Atlas databases were used to analyze the differences in mRNA and protein expression of ANGPTLs in HCC tissues. Additionally, the correlation between ANGPTL mRNA and methylation levels and clinicopathological features were assessed in the TCGA database. The correlation between ANGPTL mRNA and overall survival was determined by the Kaplan-Meier plotter. cBioPortal database was used to analyze ANGPTL genomic alterations. Genes associated with ANGPTLs were determined by enrichment with KEGG. Moreover, the differentially expressed genes of ANGPTLs were analyzed by the LinkedOmics database, and the KEGG pathway and miRNA targets of ANGPTLs were also enriched.

Results: There was a significant correlation between the ANGPTL members (excluding ANGPTL2) and the prognosis of HCC patients according to the Kaplan-Meier plotter analysis ( < 0.05). ANGPTL1 was the gene with the highest mutation frequency. ANGPTLs are involved in certain pathways that may influence the development of HCC.

Conclusion: In summary, the expression of some members of ANGPTLs was significantly correlated with HCC prognosis, suggesting that the ANGPTL gene family members may be promising molecular markers for HCC treatment and prognosis.
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http://dx.doi.org/10.1155/2022/1844352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177307PMC
June 2022

Imaging translational control by Argonaute with single-molecule resolution in live cells.

Nat Commun 2022 Jun 10;13(1):3345. Epub 2022 Jun 10.

Department of Biochemistry & Molecular Biology, Colorado State University, Fort Collins, CO, 80523, USA.

A major challenge to our understanding of translational control has been deconvolving the individual impact specific regulatory factors have on the complex dynamics of mRNA translation. MicroRNAs (miRNAs), for example, guide Argonaute and associated proteins to target mRNAs, where they direct gene silencing in multiple ways that are not well understood. To better deconvolve these dynamics, we have developed technology to directly visualize and quantify the impact of human Argonaute2 (Ago2) on the translation and subcellular localization of individual reporter mRNAs in living cells. We show that our combined translation and Ago2 tethering sensor reflects endogenous miRNA-mediated gene silencing. Using the sensor, we find that Ago2 association leads to progressive silencing of translation at individual mRNA. Silencing was occasionally interrupted by brief bursts of translational activity and took 3-4 times longer than a single round of translation, consistent with a gradual increase in the inhibition of translation initiation. At later time points, Ago2-tethered mRNAs cluster and coalesce with P-bodies, where a translationally silent state is maintained. These results provide a framework for exploring miRNA-mediated gene regulation in live cells at the single-molecule level. Furthermore, our tethering-based, single-molecule reporter system will likely have wide-ranging application in studying RNA-protein interactions.
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http://dx.doi.org/10.1038/s41467-022-30976-3DOI Listing
June 2022

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

J Transl Med 2022 Jun 7;20(1):262. Epub 2022 Jun 7.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Road No. 1277, Wuhan, 430022, Hubei, China.

Background: Previous studies have indicated that chronic emotional stressors likely participate in the occurrence of cancers. However, direct evidence connecting stress and colorectal cancer development remains almost completely unexplored.

Methods: Chronic stress mouse model was used to investigate the influence of stress on tumorigenesis. Several major agonists and antagonists of adrenergic receptors were applied to investigate the effects of β-adrenergic signaling on the development of CRC. Chromatin immunoprecipitation assays (CHIP) were used to investigate the binding of p53 and CEBPB to TRIM2 promoter. Mammosphere cultures, Cell Counting Kit-8 (CCK-8) assay, colony-formation assay, scratch wound healing assays, qPCR, immunofluorescence, coimmunoprecipitation and western blotting were used to explore the effect of stress-induced epinephrine on the CEBPB/TRIM2/P53 axis and the progress of CRC cells.

Results: In this study, we found that stress-induced epinephrine (EPI) promotes the proliferation, metastasis and CSC generation of CRC primarily through the β2-adrenergic receptor. Furthermore, our studies also confirmed that chronic stress decreased the stability of p53 protein by promoting p53 ubiquitination. Results of transcriptome sequencing indicated that TRIM2 was overexpressed in cells treated with EPI. Further studies indicated that TRIM2 could regulate the stability of p53 protein by promoting p53 ubiquitination. Finally, we further proved that CEBPB was regulated by EPI and acts as the upstream transcription factor of TRIM2.

Conclusions: Our studies proved that stress-induced EPI promotes the development and stemness of CRC through the CEBPB/TRIM2/P53 axis.
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http://dx.doi.org/10.1186/s12967-022-03467-8DOI Listing
June 2022

On the damage tolerance of 3-D printed Mg-Ti interpenetrating-phase composites with bioinspired architectures.

Nat Commun 2022 Jun 6;13(1):3247. Epub 2022 Jun 6.

Department of Materials Science and Engineering, University of California Berkeley, Berkeley, CA, 94720, USA.

Bioinspired architectures are effective in enhancing the mechanical properties of materials, yet are difficult to construct in metallic systems. The structure-property relationships of bioinspired metallic composites also remain unclear. Here, Mg-Ti composites were fabricated by pressureless infiltrating pure Mg melt into three-dimensional (3-D) printed Ti-6Al-4V scaffolds. The result was composite materials where the constituents are continuous, mutually interpenetrated in 3-D space and exhibit specific spatial arrangements with bioinspired brick-and-mortar, Bouligand, and crossed-lamellar architectures. These architectures promote effective stress transfer, delocalize damage and arrest cracking, thereby bestowing improved strength and ductility than composites with discrete reinforcements. Additionally, they activate a series of extrinsic toughening mechanisms, including crack deflection/twist and uncracked-ligament bridging, which enable crack-tip shielding from the applied stress and lead to "Γ"-shaped rising fracture resistance R-curves. Quantitative relationships were established for the stiffness and strengths of the composites by adapting classical laminate theory to incorporate their architectural characteristics.
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http://dx.doi.org/10.1038/s41467-022-30873-9DOI Listing
June 2022

Therapeutic Effects of Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells on a Murine Model of Acute Type-2-Dominated Airway Inflammation.

Stem Cell Rev Rep 2022 May 27. Epub 2022 May 27.

Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, China.

Allergic rhinitis and allergic asthma are the most common type-2 inflammatory diseases, which are hardly curable and cause heavy burden to general well-being. Mesenchymal stem cells (MSCs) are multipotent nonhematopoietic cells with potential immunomodulatory effects that have been showning to have a therapeutic effect on allergic diseases. Here, we investigated the effects of human induced pluripotent stem cell (iPSC)-derived MSCs on airway hyperresponsiveness and acute type-2-dominated inflammation throughout the upper and lower airways. In this study, human MSCs, MSC cell culture supernatant, and culture medium (control) was injected into the acute airway inflammatory model via the tail vein. Mouse behavioristics were recorded immediately and mouse lung function was measured 24 hours after the last ovalbumin (OVA) challenge. Histological staining, Luminex, Elisa and flow cytometry were employed to evaluate the effects on the production of total/OVA-specific IgG1 and IgE, cytokines expression in lung tissues, and inflammatory cells infiltration in the lung and spleen of the experimental mice. Expressions of eotaxin, IL-4, IL-5, IL-13, IL-33 in nasal and lung lavage were evaluated by Luminex and Elisa. We found that for this acute inflammatory mouse model, human MSC transplantation significantly mitigated the decreased motoring time and the increased lung function Rrs caused by OVA challenge. Serum OVA-IgG1, OVA-IgE, and eosinophil percentages in the splenocytes were significantly decreased. Injection of the MSC supernatant also showed the same trend, but not significantly changed. After treatment, IL-4 and IL-13 were significantly decreased in the lung tissue, and IL-5 and IL-13 were significantly decreased in lung lavage. In conclusion, both human MSC culture supernatant and cell transplantation could alleviate AHR and inflammation in acute inflammatory experimental animals, which demonstrated their potential for clinical therapeutics. Human iPSC-MSCs, MSC cell culture supernatant, or culture medium (control) was injected into the OVA-induced acute airway inflammatory model via the tail vein. Behavioral changes, AHR, serum OVA-specific IgG1 and IgE concentrations, and type-2 inflammations were alleviated.
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http://dx.doi.org/10.1007/s12015-022-10389-xDOI Listing
May 2022

 Variations in lignin monomer contents and stable hydrogen isotope ratios in methoxy groups during the biodegradation of garden biomass.

Sci Rep 2022 May 24;12(1):8734. Epub 2022 May 24.

Key Laboratory of Soil Resource and Biotech Applications, Shaanxi Academy of Sciences, Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), No.17, Cuihua South Road, Xi'an, 710061, China.

Lignin, a highly polymerized organic component of plant cells, is one of the most difficult aromatic substances to degrade. Selective biodegradation under mild conditions is a promising method, but the dynamic variations in lignin monomers during the biodegradation of lignocellulose are not fully understood. In this study, we evaluated the differences in lignin degradation under different microbial inoculation based on the lignin monomer content, monomer ratio, and stable hydrogen isotope ratio of lignin methoxy groups (δH). The weight loss during degradation and the net loss of lignocellulosic components improved dramatically with fungal inoculation. Syringyl monolignol (S-lignin), which contains two methoxy groups, was more difficult to degrade than guaiacyl (G-lignin), which contains only one methoxy group. The co-culture of Pseudomonas mandelii and Aspergillus fumigatus produced the greatest decrease in the G/S ratio, but δH values did not differ significantly among the three biodegradation experiments, although the enrichment was done within the fungal inoculation. The fluctuation of δH values during the initial phase of biodegradation may be related to the loss of pectic polysaccharides (another methoxy donor), which mainly originate from fallen leaves. Overall, the relative δH signals were preserved despite decreasing G/S ratios in the three degradation systems. Nevertheless, some details of lignin δH as a biomarker for biogeochemical cycles need to be explored further.
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http://dx.doi.org/10.1038/s41598-022-12689-1DOI Listing
May 2022

CUB domain containing protein 1 (CDCP1) is a target for radioligand therapy in castration resistant prostate cancer including PSMA null disease.

Clin Cancer Res 2022 May 23. Epub 2022 May 23.

University of California, San Francisco, San Francisco, CA, United States.

Purpose: With the improvement in overall survival with 177Lu-PSMA 617, radioligand therapy (RLT) is now a viable option for patients with metastatic castration resistant prostate cancer (mCRPC). However, responses are variable, in part due to low PSMA expression in 30% of patients. Herein, we evaluated if the cell surface protein CUB domain containing protein 1 (CDCP1) can be exploited to treat mCRPC with RLT, including in PSMA low subsets.

Experimental Design: CDCP1 levels were evaluated using RNA-seq from 119 mCRPC biopsies. CDCP1 levels were assessed in 17 post enzalutamide or abiraterone treated mCRPC biopsies, 12 patient derived xenografts (PDX), and prostate cancer cell lines. 4A06, a recombinant human antibody that targets the CDCP1 ectodomain, was labeled with Zr-89 or Lu-177 and tested in tumor bearing mice.

Results: CDCP1 expression was observed in 90% of mCRPC biopsies, including small cell neuroendocrine (SCNC) and adenocarcinomas with low FOLH1 (PSMA) levels. Fifteen of 17 evaluable mCRPC biopsies (85%) demonstrated membranous CDCP1 expression, and 4 of 17 (23%) had higher CDCP1 H-scores compared to PSMA. CDCP1 was expressed in ten of twelve PDX samples. Bmax values of ~22,000, ~6,200, and ~2,800 fmol/mg were calculated for PC3, DU145, and C4-2B human prostate cancer cells respectively. 89Zr-4A06 PET detected six human prostate cancer xenografts, including PSMA low tumors. 177Lu-4A06 significantly suppressed growth of DU145 and C4-2B xenografts.

Conclusions: The data provide the first evidence supporting CDCP1-directed RLT to treat mCRPC. Expanded studies are warranted to determine if CDCP1 is a viable drug target for mCPRC patients.
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http://dx.doi.org/10.1158/1078-0432.CCR-21-3858DOI Listing
May 2022

LiNiCoMnO Cathodes Coated with Dual-Conductive Polymers for High-Rate and Long-Life Solid-State Lithium Batteries.

ACS Appl Mater Interfaces 2022 Jun 20;14(21):24929-24937. Epub 2022 May 20.

College of Physics, Qingdao University, Qingdao 266071, China.

High-energy density and safe solid lithium batteries call for cathodes with high capacity and good kinetic properties. In this work, LiNiCoMnO (NCM622) cathodes are coated with the ionic-electronic dual-conductive polymers composed of poly(ethylene glycol) (PEG)-doped polyaniline (PANI). Scanning electron microscopy, transmission electron microscopy, Fourier transform infrared, and thermogravimetric analysis reveal that the dual-conductive polymers are homogeneously coated on the surfaces of NCM622 cathodes with a thickness of approximately 10 nm. The solid-state lithium batteries consisting of the NCM622 cathodes coated with PANI-PEG show a specific capacity of 158 mA h g and a retention rate of 88% after 100 cycles at the rate of 0.1 C and room temperature, which are superior to the discharge capacity of 153 mA h g and capacity retention of 59% after 100 cycles for the batteries with the pristine NCM622 cathodes. Moreover, the cells with the coated cathodes display a better rate performance of 84 mA h g at 1 C than those with the uncoated ones which show a rate performance of 11 mA h g at 1 C.
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http://dx.doi.org/10.1021/acsami.2c03618DOI Listing
June 2022

Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodontic‑induced external apical root resorption in a rat model.

Mol Med Rep 2022 Jul 20;26(1). Epub 2022 May 20.

Department of Orthodontics, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.

The aim of the present study was to investigate the alterations in the formation of cementocytes in response to orthodontic forces and to evaluate the contribution of these cells in the biological changes of tooth movement and associated root resorption. A total of 90 Sprague Dawley rats were randomly assigned to the control, high force, and low force groups. Intrusion forces of 10 and 50 g were applied on the rat molar to induce tooth intrusion. The tooth movement was observed from 0 to 14 days by micro‑computed tomography, bone histometric analysis, tartrate‑resistant acid phosphatase staining, as well as reverse transcription‑quantitative PCR and immunofluorescence staining assays. The results suggested that under low force conditions, osteoclasts were distributed at a higher frequency on the bone side than on the root side. Under high force conditions, both sides suffered osteoclast infiltration. In the low force group, the cementocytes exhibited downregulated sclerostin () and osteoprotegerin () mRNA levels and a lower receptor activator of nuclear factor‑κB ligand ()/OPG ratio over a certain period of time. The expression levels of these genes were lower compared with those of the osteocytes at each time‑point. In the high force group, both cementocytes and osteocytes upregulated the and ratio on days 7 and 14, while the cementocytes expressed higher levels of mRNA than those noted in the osteocytes. These data suggested that cementocytes responded to the orthodontic force via modulation of the ratio and expression. The biological response of cementocytes contributed to the mechanotransduction and homoeostasis of the roots under compression. Excessive forces may act as a negative factor of this regulatory role. These results expand our knowledge on the function of cementocytes.
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http://dx.doi.org/10.3892/mmr.2022.12744DOI Listing
July 2022

Ultrafast Solar-Vapor Harvesting Based on a Hierarchical Porous Hydrogel with Wettability Contrast and Tailored Water States.

ACS Appl Mater Interfaces 2022 Jun 17;14(21):24766-24774. Epub 2022 May 17.

Beijing National Laboratory for Molecular Sciences, Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Optimizing the water bonding network in an evaporator is significant for efficient solar-driven vapor generation (SVG). Herein, we report a facile one-pot method to regulate the hydrated structure and wettability in a hierarchical porous hydrogel. An ovalbumin (OVA)-polyacrylamide hydrogel foam was fabricated in a cake-making fashion. Because of the enrichment of amphiphilic OVA at the interface, the hydrophobic walls of the air pores in the foam provide vaporization sites and help reduce parasitic heat loss, while the hydrophilic skeleton with the secondary pores effectively pumps capillary water. Notably, the proportion of intermediate water in the foam reaches 87.6% with the melting point as low as -10 °C. All these features contribute to an exceptional evaporation rate of 3.4-4.5 kg m h under 1 sun and robust SVG performances at high-humidity, weak sunlight, or cold weathers. The strategy of using amphiphilic molecules to optimize the hydrated structures both at the interface and in bulk promises the reasonable design of SVG materials with superior efficiency and weather adaptability.
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http://dx.doi.org/10.1021/acsami.2c03597DOI Listing
June 2022

Three-dimensional printed lithium iron phosphate coated with magnesium oxide cathode with improved areal capacity and ultralong cycling stability for high performance lithium-ion batteries.

J Colloid Interface Sci 2022 Oct 10;623:168-181. Epub 2022 May 10.

Additive Manufacturing Institute, College of Mechatronics & Control Engineering, Shenzhen University, Shenzhen 518060, China; Guangdong Key Laboratory of Electromagnetic Control and Intelligent Robots, College of Mechatronics & Control Engineering, Shenzhen University, 518060, China.

Three-dimensional (3D) printing of Li-ion batteries with unconventional 3D electrodes has attracted considerable attention in recent years. However, fabricating 3D electrodes with high specific capacity, high areal capacity, ultralong cycling stability, and improved rate performance remains a challenge to date. Novel 3D grid-patterned [email protected] composite electrodes with thicknesses of 143, 306, and 473 μm were fabricated via 3D printing. The electrochemical performance of half cells was evaluated. The 3D-printed [email protected] (143 μm) electrodes exhibit stable specific capacities of 142.8 mAh g @ 1.0 C and 90.3 mAh g @ 10.0 C after 800 and 1700 cycles, respectively. In addition, the 473 μm-thick 3D grid-patterned [email protected] achieves an areal capacity of 3.01 mAh cm @ 0.1 C after 20 cycles. The full cells comprised 143 μm-thick 3D-printed [email protected], and 217 μm LiTiO electrodes show a capacity of 139.0 mAh g @ 1.0 C after 400 cycles. These results indicate that, this type of thick 3D-printed [email protected] electrode achieves high capacity, high-rate capability, and ultralong cycle stability. The outstanding performance ascribes the fast electrolyte infusion of 3D-printed electrodes and the enhanced electronic/ionic transport.
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http://dx.doi.org/10.1016/j.jcis.2022.05.030DOI Listing
October 2022

FAM96A suppresses epithelial-mesenchymal transition and tumor metastasis by inhibiting TGFβ1 signals.

Life Sci 2022 Jul 2;301:120607. Epub 2022 May 2.

Peking University Center for Human Disease Genomics, Beijing, China; Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing, China; NHC Key Laboratory of Medical Immunology, Peking University, Beijing, China. Electronic address:

Metastasis is the main cause of death in 90% of patients with tumors, and epithelial-mesenchymal transition (EMT) plays a key role in this process. Family with sequence similarity 96 member A (FAM96A) is an evolutionarily conserved protein related to cytosolic iron assembly. However, no research has been conducted on its role in tumor metastasis. Bioinformatics analysis with Kaplan-Meier analysis showed that patients with lower FAM96A expression in different tumors had shorter survival times and poorer prognoses. Here, we identified FAM96A as a crucial regulator of the TGFβ signaling pathway because it inhibits TGFβ-mediated tumor metastasis and EMT. FAM96A did not affect the proliferation or apoptosis of tumor cells but significantly reduced their migration and invasion abilities in vitro. The colonization and metastatic abilities of FAM96A-knockout tumor cells were significantly enhanced in vivo. Furthermore, the overexpression of exogenous FAM96A inhibited TGFβ-induced EMT through the SMAD-mediated pathway and downregulated the expression of endogenous transforming growth factor-β1 (TGFβ1). These findings demonstrated a correlation between EMT and FAM96A gene expression for the first time, which is highly important for revealing the mechanism underlying tumor metastasis.
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http://dx.doi.org/10.1016/j.lfs.2022.120607DOI Listing
July 2022

Ratiometric fluorescence probe of Cu and biothiols by using carbon dots and copper nanoclusters.

RSC Adv 2021 Oct 15;11(53):33662-33674. Epub 2021 Oct 15.

School of Pharmacy, Shenyang Pharmaceutical University 103 Wenhua Road Shenhe District Shenyang Liaoning 110016 P. R. China +86-24-4352-0571 +86-24-4352-0571.

A novel dual-emission ratiometric fluorescent probe based on N-doped yellow fluorescent carbon dots (y-CDs) and blue fluorescent copper nanoclusters (CuNCs) was established for quantitative determination of Cu and biothiols. In this work, the Cu-(y-CDs) complexes formed by the chelation of y-CDs with Cu, showed an absorption peak at 430 nm that not only enhanced the fluorescence of y-CDs through inhibiting photoinduced electron transfer (PET) but also effectively quenched the fluorescence of CuNCs due to Förster resonance energy transfer (FRET). In addition, the chelation of y-CDs with Cu could be inhibited by biothiols that prevented the fluorescence of y-CDs from being enhanced and the fluorescence of CuNCs from being quenched. On account of the changes of ratiometric signal, a dual-emission fluorescence probe for Cu and biothiols determination was achieved. The proposed method exhibited high sensitivity for Cu and biothiols in the ranges of 0.5-100 μM and 0.8-50 μM and the limits of detection (LODs) of Cu, glutathione (GSH), cysteine (Cys) and homocysteine (Hcy) were 0.21 μM, 0.33 μM, 0.39 μM and 0.46 μM, respectively. Subsequently, the established strategy presented an application prospect for the detection of Cu and biothiols in real samples.
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http://dx.doi.org/10.1039/d1ra05854aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042249PMC
October 2021

Identification of an autophagy-related gene signature for predicting prognosis and immune activity in pancreatic adenocarcinoma.

Sci Rep 2022 04 29;12(1):7006. Epub 2022 Apr 29.

Institute of Health Service and Transfusion Medicine, Beijing, 100850, People's Republic of China.

Adenocarcinoma of the pancreas (PAAD) is a cancerous growth that deteriorates rapidly and has a poor prognosis. Researchers are investigating autophagy in PAAD to identify a new biomarker and treatment target. An autophagy-related gene (ARG) model for overall survival (OS) was constructed using multivariate Cox regression analyses. A cohort of the Cancer Genome Atlas (TCGA)-PAAD was used as the training group as a basis for model construction. This prediction model was validated with several external datasets. To evaluate model performance, the analysis with receiver operating characteristic curves (ROC) was performed. The Human Protein Atlas (HPA) and Cancer Cell Line Encyclopedia (CCLE) were investigated to validate the effects of ARGs expression on cancer cells. Comparing the levels of immune infiltration between high-risk and low-risk groups was finished through the use of CIBERSORT. The differentially expressed genes (DEGs) between the low-/high-risk groups were analyzed further via Gene Ontology biological process (GO-BP) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, which were used to identify potential small-molecule compounds in Connectivity Map (CMap), followed by half-maximal inhibitory concentration (IC50) examination with PANC-1 cells. The risk score was finally calculated as follows: BAK1 × 0.34 + ITGA3 × 0.38 + BAG3 × 0.35 + APOL1 × 0.26-RAB24 × 0.67519. ITGA3 and RAB24 both emerged as independent prognostic factors in multivariate Cox regression. Each PAAD cohort had a significantly shorter OS in the high-risk group than in the low-risk group. The high-risk group exhibited infiltration of several immune cell types, including naive B cells (p = 0.003), plasma cells (p = 0.044), and CD8 T cells (nearly significant, p = 0.080). Higher infiltration levels of NK cells (p = 0.025), resting macrophages (p = 0.020), and mast cells (p = 0.007) were found in the high-risk group than the low-risk group. The in vitro and in vivo expression of signature ARGs was consistent in the CCLE and HPA databases. The top 3 enriched Gene Ontology biological processes (GO-BPs) were signal release, regulation of transsynaptic signaling, and modulation of chemical synaptic transmission, and the top 3 enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were MAPK, cAMP, and cell adhesion molecules. Four potential small-molecule compounds (piperacetazine, vinburnine, withaferin A and hecogenin) that target ARGs were also identified. Taking the results together, our research shows that the ARG signature may serve as a useful prognostic indicator and reveal potential therapeutic targets in patients with PAAD.
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http://dx.doi.org/10.1038/s41598-022-11050-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054801PMC
April 2022

Efficacy of intravitreal conbercept combined with panretinal photocoagulation for severe nonproliferative diabetic retinopathy without macular edema.

Int J Ophthalmol 2022 18;15(4):615-619. Epub 2022 Apr 18.

Department of Ophthalmology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.

Aim: To assess efficacy of intravitreal conbercept (IVC) injection in combination with panretinal photocoagulation (PRP) PRP alone in patients with severe nonproliferative diabetic retinopathy (SNPDR) without macular edema (ME).

Methods: Forty-eight patients with SNPDR without ME (56 eyes) were divided into the PRP group and IVC+PRP group (the pulse group) in this retrospective clinical study. Conbercept was intravitreally administered to patients in the pulse group 1wk before treatment with PRP and followed up for 1, 3, and 6mo. The best-corrected visual acuity (BCVA, logMAR), center foveal thickness (CFT), visual acuity (VA) improvement, and adverse reactions were compared between groups.

Results: In the PRP group, the BCVA reduced at 1 and 3mo before improving at 6mo. In the pulse group, baseline BCVA decreased continuously at 1mo, increased at 3 and 6mo. BCVA in the pulse group was better than that in the PRP group at 1, 3, and 6mo. There was an increase in CFT in the PRP group during follow-up compared with baseline. In the pulse group, CFT was increased at 1mo relative to baseline, steadily decreased to the baseline level at 3 and 6mo. There was a more significant reduction in CFT in the pulse group during follow-up compared with the PRP group. The effective rates of VA in the PRP and the pulse groups were 81.48% and 100%, respectively.

Conclusion: As PRP pretreatment, a single dose of IVC administration has beneficial effects for preventing PRP-induced foveal thickening and increasing VA in patients with SNPDR without ME.
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http://dx.doi.org/10.18240/ijo.2022.04.15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995731PMC
April 2022

Blockage of transient receptor potential vanilloid 4 prevents postoperative atrial fibrillation by inhibiting NLRP3-inflammasome in sterile pericarditis mice.

Cell Calcium 2022 06 10;104:102590. Epub 2022 Apr 10.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Research Center of Ion Channelopathy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Key Lab for Biological Targeted Therapy of Education Ministry and Hubei Province, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

The incidence of atrial fibrillation (AF) increases after surgery and is associated with the activation of NLRP3-inflammation. Our previous studies have found that transient receptor potential vanilloid 4 (TRPV4) blockade reduces the susceptibility to AF, but its molecular mechanisms remains unclear. Therefore, we hypothesized that blockage of TRPV4 reduces the incidence of AF by inhibiting NLRP3-inflammasome in sterile pericarditis (SP) mice. In this study, we established SP mice by dusting talcum powder on atrial surfaces. We first confirmed that genetic or pharmacological TRPV4 inhibition reduced the susceptibility to AF in SP mice. We also found that the expression level of NLRP3-inflammasome and inflammatory cytokines significantly increased in the atria of SP mice, which further increased in application the TRPV4 agonist GSK1016790A (GSK101) and decreased in application the TRPV4 antagonist GSK2193874. More importantly, ERK inhibitor (U0126) or NF-κB inhibitor (Bay11-7082) could partially reverse GSK101-induced NLRP3-inflammasome up-regulation. Interestingly, U0126 can reversed GSK101-induced NF-κB phosphorylation, but Bay11-7082 cannot change GSK101-induced ERK phosphorylation. Finally, we shown that the activation of NLRP3-inflammasome and ERK/NF-κB signaling pathway significantly reduced in TRPV4-knockout SP mice. Collectively, our studies indicate that blockage of TRPV4 prevents AF in SP mice by inhibiting NLRP3-inflammasome through the ERK/NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.ceca.2022.102590DOI Listing
June 2022

Patients' Willingness of First Visit in Primary Medical Institutions and Policy Implications: A National Cross-Sectional Survey in China.

Front Public Health 2022 1;10:842950. Epub 2022 Apr 1.

School of Public Health, Capital Medical University, Beijing, China.

Background: The Chinese hierarchical treatment system expects patients to first visit primary medical institutions (PMIs), and patients' willingness determined their utilization of primary health care. The aim of this study was to explore the factors associated with patients' willingness to make their first visit to PMIs.

Methods: We employed multistage stratified sampling and convenience sampling to administer questionnaires to 1,507 patients in Beijing, Qinghai, and Fujian. Patients' willingness of first visit in PMIs was analyzed using Chi-square test and binary logistic regression.

Results: Of the 1,507 participants in the survey, 55.1% were willing to make their first visit in PMIs. Fewer patients in Beijing (17.6%) are willing to make their first visit in PMIs than those in Qinghai (71.9%) and Fujian provinces (72.0%). Binary logistic regression analysis revealed that higher recognition of the community first visit policy and higher satisfaction with the medical technology of PMIs are associated with patients' willingness of first visit in PMIs.

Conclusions: Due to differences in local economic conditions, medical resources, and policy formulation, there are differences among provinces in patients' willingness of first visit in PMIs. To increase patients' rate of visits in PMIs, it is important to improve service capacity and quality of PMIs and change residents' attitudes for PMIs.
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http://dx.doi.org/10.3389/fpubh.2022.842950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010779PMC
April 2022

Dengue Fever in Mainland China, 2005-2020: A Descriptive Analysis of Dengue Cases and Data.

Int J Environ Res Public Health 2022 03 25;19(7). Epub 2022 Mar 25.

Division of Infectious Disease Management, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Dengue fever occurs throughout mainland China, except in the Tibet Autonomous Region. During 2005-2020, there were 12,701 imported cases and 81,653 indigenous cases recorded. The indigenous cases were mainly clustered in Guangdong (74.0%) and Yunnan provinces (13.7%). Indigenous dengue fever is a seasonal illness in mainland China, manifesting predominantly in summer and autumn. Indigenous dengue fever cases tend to peak every 5years and have shown a substantial increase during the period 2005-2020. During the study period, indigenous dengue fever occurred more than ten times in each of the seven counties of Guangdong Province. Indigenous dengue fever has spread from low to high latitudes; that is, from the southwestern, southern, and southeastern areas to the central and northern regions, and from border ports and cities to rural areas. has become widespread in Yunnan Province but has diminished in Guangxi, Guangdong, and Hainan provinces in recent years. is distributed throughout mainland China, spanning 25 provinces and municipalities. To maintain effective public health prevention and control, it is important to monitor dengue occurrence, provide dengue classification guidance, and ensure sustainable vector management of .
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http://dx.doi.org/10.3390/ijerph19073910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997546PMC
March 2022

Expression Pattern of Entire Cytochrome P450 Genes and Response of Defenses in a Metabolic-Herbicide-Resistant Biotype of .

Front Plant Sci 2022 25;13:868807. Epub 2022 Mar 25.

Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, China.

Enhanced herbicide metabolism mediated by cytochrome P450s has been proposed as one of the major mechanisms of resistance to fenoxaprop--ethyl in a metabolic-herbicide-resistant biotype of Asia minor bluegrass ( Nees ex Steud.). Upon pre-treatment with the P450 inhibitor piperonyl butoxide, a remarkable reduction in metabolic rates of the phytotoxic fenoxaprop- has been observed in the resistant plants, implying that constitutive and/or fenoxaprop--ethyl-induced up-regulation of specific P450 isoforms are involved in the fenoxaprop--ethyl resistance. However, which P450 gene(s) were responsible for the metabolic resistance is still unknown. In this present study, based on the abundant gene resources of established previously, a total of 48 putative P450 genes were isolated from the metabolic-herbicide-resistant plants and used for gene expression analysis. The most suitable reference genes for accurate normalization of real-time quantitative PCR data were first identified in and recognized as actin (), 18S rRNA (), and ribulose-1,5-bisphosphate carboxylase oxygenase () under fenoxaprop--ethyl stress, glyceraldehyde-3-phosphate dehydrogenase () and elongation factor 1α () under mesosulfuron-methyl stress, and , , eukaryotic initiation factor 4a (), and 25S rRNA () at different growth stages. Expression analysis of the putative P450 genes revealed that six genes, respectively, annotated as , , , , , and were up-regulated more than 10-fold in the resistant plants by fenoxaprop--ethyl treatment, and all of them exhibited constitutively and/or herbicide-induced higher transcript levels in the fenoxaprop--ethyl-resistant than in the susceptible plants. Three genes, respectively, annotated as , , and constantly up-regulated in the resistant than in the susceptible plants after fenoxaprop--ethyl treatment. Up-regulated expressions of these specific P450 genes were consistent with the higher P450 contents determined in the resistant plants. These results will help to elucidate the mechanisms for P450-mediated metabolic-herbicide resistance in as well as other grass weed species.
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http://dx.doi.org/10.3389/fpls.2022.868807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990753PMC
March 2022

Hydroxyl-Terminated Polybutadiene-Based Polyurethane with Self-Healing and Reprocessing Capabilities.

ACS Omega 2022 Mar 15;7(12):10156-10166. Epub 2022 Mar 15.

College of Material Science and Engineering, Guilin University of Technology, Guilin 541004, P. R. China.

Hydroxyl-terminated polybutadiene (HTPB)-based polyurethane (PU) networks play indispensable roles in a variety of applications; however, they cannot be reprocessed, resulting in environmental problems and unsustainable industrial development. In this work, recyclable HTPB-based PU vitrimer (HTPB-PU) networks are fabricated by introduction of a cross-linker 2,2'-(1,4-phenylene)-bis[4-mercaptan-1,3,2-dioxaborolane] (BDB) with dynamic boronic ester bonds into the network. Meanwhile, the BDB can stabilize the HTPB unit in the network by elimination of double bonds. The novel HTPB-PU networks are constructed by a thiol-ene "click" reaction and an addition reaction between HTPB and cross-linker BDB and isocyanates (HDI). The dynamic HTPB-PU networks are characterized by dynamic mechanical analysis (DMA) and Fourier transform infrared (FTIR). The obtained dynamic HTPB-PU networks possess superior thermostability. Moreover, due to the presence of dynamic boronic ester bonds, the HTPB-PU network topologies can be altered, contributing to the reprocessing, self-healing, and welding abilities of the final polymer. Through a hot press, the pulverized sample can be reprocessed for several cycles, and mechanical properties of the reprocessed samples are similar to those of the pristine one, with the tensile strength being even higher. The self-healed sample exhibits almost complete recovery from scratch after the healing treatment at 130 °C for 3 h. Moreover, a welding efficiency of 120% was achieved.
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http://dx.doi.org/10.1021/acsomega.1c06416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973043PMC
March 2022

Mutations and Phylogenetic Analyses of SARS-CoV-2 Among Imported COVID-19 From Abroad in Nanjing, China.

Front Microbiol 2022 17;13:851323. Epub 2022 Mar 17.

Microbiology Laboratory, Nanjing Municipal Center for Disease Control and Prevention, Nanjing, China.

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a pandemic and is threatening human health globally. The rapid genome sequencing and bioinformatic analysis of SARS-CoV-2 have become a helpful tool in the battle against the COVID-19. Here, we report the genetic characteristics, variations and phylogenetic analysis of SARS-CoV-2 sequenced from 42 clinical specimens. The complete genomes sequencing of SARS-CoV-2 were performed using Oxford Nanopore sequencing. All genomes accumulated mutations compared to the Wuhan-Hu-1 (GenBank Accession No: MN908947.3). Our data of the 42 whole genomes revealed 16 different lineages. The B.1.1 lineage was the most frequent, and 5, 2, 2, 3, and 1 sequences were classified as lineages of B.1.1.7, B.1.351, P.1, B.1.617.2, and C.37, respectively. A total of 328 nucleotide mutation sites were found in 42 genomes, among which A23403G mutation (D614G amino acid change in the spike protein) was the most common substitution. The phylogenetic trees of 42 SARS-CoV-2 sequences and GISAID-available SARS-CoV-2 sequences were constructed and its taxonomic status was supported. These results will provide scientific basis for tracing the source and prevention and control of SARS-CoV-2 imported from abroad in Nanjing, China.
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http://dx.doi.org/10.3389/fmicb.2022.851323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969601PMC
March 2022

Knockdown of circ_CDYL Contributes to Inhibit Angiotensin II-Induced Podocytes Apoptosis in Membranous Nephropathy via the miR-149-5p/TNFSF11 Pathway.

J Cardiovasc Pharmacol 2022 Jun 1;79(6):887-895. Epub 2022 Jun 1.

Department of Nephrology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou City, China.

Abstract: Circular RNAs (circRNAs) have been verified as vital regulators in various diseases, including membranous nephropathy (MN). Therefore, the role of circ_CDYL in podocyte apoptosis and MN was investigated. The real-time quantitative polymerase chain reaction was performed to measure the expression of circ_CDYL, microRNA-149-5p (miR-149-5p), and tumor necrosis factor superfamily member 11 (TNFSF11) in podocytes. In addition, angiotensin II (Ang II) was used to induce apoptosis of podocytes. The apoptosis-related protein expression was quantified by western blot assay. The apoptosis of podocytes was evaluated by flow cytometry assay. The interaction relationship between miR-149-5p and circ_CDYL or TNFSF11 was confirmed by dual-luciferase reporter assay. Circ_CDYL was significantly overexpressed in MN patients and Ang II-induced podocytes compared with control groups. Importantly, loss-of-functional experiments indicated that knockdown of circ_CDYL protected podocytes from Ang II-induced apoptosis. MiR-149-5p was verified as target of circ_CDYL and negatively correlated with circ_CDYL expression in MN patients. Knockdown of circ_CDYL-mediated effects on Ang II-induced podocyte cells were abolished by silencing miR-149-5p. Besides, the upregulation of miR-149-5p could suppress apoptosis in Ang II-induced podocyte cells by targeting TNFSF11. Under Ang II stimulation, the upregulation of TNFSF11 could increase the expression of TNFSF11 and induce apoptosis in circ_CDYL-silencing podocytes. Our results confirmed that circ_CDYL specifically targeted miR-149-5p/TNFSF11 pathway to regulate Ang II-induced apoptosis in podocytes, which might be useful diagnostic biomarkers in MN.
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http://dx.doi.org/10.1097/FJC.0000000000001262DOI Listing
June 2022

A cytochrome P450 superfamily gene, IbCYP82D47, increases carotenoid contents in transgenic sweet potato.

Plant Sci 2022 May 23;318:111233. Epub 2022 Feb 23.

Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address:

The cytochrome P450 superfamily (CYP450) is one of the largest protein families in plants, and its members play diverse roles in primary and secondary metabolic biosynthesis. In this study, the CYP450 family gene IbCYP82D47 was cloned from the high carotenoid line HVB-3 of sweet potato (Ipomoea batatas). The IbCYP82D47 protein harbored two transmembrane domains and dynamically localized between plastid stroma and membrane. Overexpression of IbCYP82D47 not only increased total carotenoid, lutein, zeaxanthin and violaxanthin contents by 32.2-48.0%, 10.5-13.3%, 40.2-136% and 82.4-106%, respectively, but also increased the number of carotenoid globules in sweet potato storage roots. Furthermore, genes associated with the carotenoid biosynthesis (IbDXS, IbPSY, IbLCYE, IbBCH, IbZEP) were upregulated in transgenic sweet potato. In addition, IbCYP82D47 physically interacts with geranylgeranyl diphosphate synthase 12 (IbGGPPS12). Our findings suggest that IbCYP82D47 increases carotenoid contents by interacting with the carotenoid biosynthesis related protein IbGGPPS12, and influencing the expressions of carotenoid biosynthesis related genes in transgenic sweet potato.
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http://dx.doi.org/10.1016/j.plantsci.2022.111233DOI Listing
May 2022
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