Publications by authors named "Ning Zhao"

596 Publications

Exploring the catalytic mechanism of a novel β-glucosidase BGL0224 from Oenococcus oeni SD-2a: Kinetics, spectroscopic and molecular simulation.

Enzyme Microb Technol 2021 Aug 3;148:109814. Epub 2021 May 3.

College of Food Science and Engineering, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address:

The β-glucosidase derived from microorganisms has attracted worldwide interest for their industrial applications, but studies on β-glucosidases from Oenococcus oeni are rare. In this paper, catalytic mechanism of a novel β-glucosidase BGL0224 of Oenococcus oeni SD-2a was explored for the first time by kinetic parameters determination, fluorescence spectroscopy and quenching mechanism analysis, molecular dynamics simulation. The results indicated that BGL0224 had universal catalytic effect on different types of glycoside substrates, but the catalytic efficiencies were different. Fluorescence quenching analysis results suggested that the quenching processes between BGL0224 and seven kinds of substrates were predominated by the static quenching mechanism. A reasonable three-dimensional model of BGL0224 was obtained using the crystal structure of E.coli BglA as a template. The analysis results of molecular simulation (RMSD, Rg, RMSF and hydrogen bonding) showed that the composite system 'BGL0224-pNPG' was very stable after 40 ns. The catalytic process of BGL0224 acting on 'p-Nitrophenyl β-d-glucopyranoside' conformed to the double displacement mechanism. Two glutamic acid residues 'Glu178 and Glu377' played a vital role in the whole catalytic process. Overall, this study gave specific insights on the catalytic mechanism of BGL0224, which was of great significance for developing its potential applications in food industry.
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http://dx.doi.org/10.1016/j.enzmictec.2021.109814DOI Listing
August 2021

Conversion of mouse embryonic fibroblasts into neural crest cells and functional corneal endothelia by defined small molecules.

Sci Adv 2021 Jun 4;7(23). Epub 2021 Jun 4.

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Laboratory, Beijing 100730, China.

Reprogramming of somatic cells into desired functional cell types by small molecules has vast potential for developing cell replacement therapy. Here, we developed a stepwise strategy to generate chemically induced neural crest cells (ciNCCs) and chemically induced corneal endothelial cells (ciCECs) from mouse fibroblasts using defined small molecules. The ciNCCs exhibited typical NCC features and could differentiate into ciCECs using another chemical combination in vitro. The resulting ciCECs showed consistent gene expression profiles and self-renewal capacity to those of primary CECs. Notably, these ciCECs could be cultured for as long as 30 passages and still retain the CEC features in defined medium. Transplantation of these ciCECs into an animal model reversed corneal opacity. Our chemical approach for direct reprogramming of mouse fibroblasts into ciNCCs and ciCECs provides an alternative cell source for regeneration of corneal endothelia and other tissues derived from neural crest.
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http://dx.doi.org/10.1126/sciadv.abg5749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177713PMC
June 2021

Exosomes Derived From Alveolar Epithelial Cells Promote Alveolar Macrophage Activation Mediated by miR-92a-3p in Sepsis-Induced Acute Lung Injury.

Front Cell Infect Microbiol 2021 10;11:646546. Epub 2021 May 10.

Department of Critical Care Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Acute lung injury (ALI) induced by sepsis is characterized by disruption of the epithelial barrier and activation of alveolar macrophages (AMs), which leads to uncontrolled pulmonary inflammation. However, effective treatments for ALI are unavailable. The exact mechanism by which the initial mediator of alveolar epithelial cells (AECs) induces inflammation remains elusive. Here we investigated the roles of AEC-derived exosomes in AM activation and sepsis-induced ALI and . Cecal ligation and puncture (CLP) was utilized to establish septic lung injury model in rats. The effect of exosomal inhibition by intratracheal GW4869 administration on lung injury was investigated. To assess the effects of AEC-derived exosomes on ALI, we treated the rat alveolar epithelial cell line RLE-6TN with LPS to induce cell damage. Exosomes from conditioned medium of LPS-treated AECs (LPS-Exos) were isolated by ultracentrifugation. The miRNAs in LPS-Exos were screened by miRNA expression profile analysis. The effects of miR-92a-3p on the function of AMs were studied. We found that intratracheal GW4869 administration ameliorated lung injury following CLP-induced ALI. LPS-Exos were taken up by AMs and activated these cells. Consistently, administration of LPS-Exos in rats significantly aggravated pulmonary inflammation and alveolar permeability. Moreover, miR-92a-3p was enriched in LPS-Exos and could be delivered to AMs. Inhibition of miR-92a-3p in AECs diminished the proinflammatory effects of LPS-Exos and . Mechanistically, miR-92a-3p activates AMs along with pulmonary inflammation. This process results in activation of the NF-κB pathway and downregulation of PTEN expression, which was confirmed by a luciferase reporter assay. In conclusion, AEC-derived exosomes activate AMs and induce pulmonary inflammation mediated by miR-92a-3p in ALI. The present findings revealed a previously unidentified role of exosomal miR-92a-3p in mediating the crosstalk between injured AEC and AMs. miR-92a-3p in AEC exosomes might represent a novel diagnostic biomarker for ALI, which may lead to a new therapeutic approach.
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http://dx.doi.org/10.3389/fcimb.2021.646546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141563PMC
May 2021

Newly Constructed NiCoO Derived from ZIF-67 with Dual Mimic Enzyme Properties for Colorimetric Detection of Biomolecules and Metal Ions.

ACS Appl Mater Interfaces 2021 Jun 21;13(21):25044-25052. Epub 2021 May 21.

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, No.94 Weijin Road, Tianjin300071, China.

Integration of novel bio-/nanostructures as effective sensing platforms is still of great significance for robust and rapid analysis. Herein, a novel metal-organic framework-derived NiCoO was synthesized via a feasible templating method. Significantly, redox couples of both Ni/Ni and Co/Co provided richer oxidation-reduction reactions, thereby leading to an enhanced catalytic activity. Furthermore, NiCoO as an enzyme mimic with peroxidase-like activity and oxidase-like activity could oxidize colorless thylbenzidine (TMB) to blue oxTMB in the absence of HO. Thus, a sensitive chromogenic sensing platform for detecting Fe, thiourea, cysteine (Cys), and epigallocatechin-3-gallate (EGCG) was proposed. The colorimetric detection methods exhibited great features of low limit of detection (LOD) and broad linear range. Owing to the complexation reaction, the chromogenic sensing system of TMB + NiCoO + Cys achieved effective detection of Cu and Mn with the LODs of 0.0022 and 0.0181 mM, respectively. Developed detection methods with wide linear ranges of 0.008-0.1 mM for Cu and 0.08-1 mM for Mn had excellent practical potential. Similarly, the reaction system of TMB + NiCoO + EGCG could achieve the colorimetric detection of Cu and Fe. The great chromogenic sensing performance for detecting Cu and Fe with a broad linear range and a low LOD could be also realized.
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http://dx.doi.org/10.1021/acsami.1c06705DOI Listing
June 2021

The prevalence of anti-neurofascin-155 antibodies in patients with neuromyelitis optica spectrum disorders.

Clin Exp Immunol 2021 May 17. Epub 2021 May 17.

Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China.

Anti-neurofascin-155 (NF155) antibodies have been observed in two cases with neuromyelitis optica spectrum disorders (NMOSD). This study investigated the prevalence of anti-NF155 antibodies in patients with NMOSD and the clinical features of anti-NF155 antibody-positive patients. Sera from 129 patients with NMOSD were screened with anti-NF155 antibodies by cell-based assay (CBA) and re-examined using immunostaining of teased mouse sciatic nerve fibres. Fifty-six patients with multiple sclerosis (MS) and 50 healthy controls (HC) were also enrolled for detecting anti-NF155 antibodies. A total of 12.40% (16 of 129) of patients with NMOSD were positive for anti-NF155 antibodies confirmed by both CBA and immunostaining. Immunoglobulin (Ig) G1 was the predominant subclass. However, none of 56 MS patients or 50 HC were positive for anti-NF155 antibodies. Anti-NF155 antibody-positive NMOSD patients had a higher proportion of co-existing with autoimmune diseases (p < 0.001) and higher positive rates of serum non-organ-specific autoantibodies, including anti-SSA antibodies (p < 0.001), anti-SSB antibodies (p = 0.008), anti-Ro-52 antibodies (p < 0.001) and rheumatoid factor (p < 0.001). Five anti-NF155 antibody-positive NMOSD patients who took part in the nerve conduction study showed mildly abnormal results. Differences in some nerve conduction study parameters were observed between anti-NF155 antibody-positive and negative patients. Anti-NF155 antibodies occurred in a small proportion of NMOSD patients. Anti-NF155 antibody-positive NMOSD patients tended to co-exist with autoimmune diseases.
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http://dx.doi.org/10.1111/cei.13617DOI Listing
May 2021

Role of Indole-3-Acetic Acid in NAFLD Amelioration After Sleeve Gastrectomy.

Obes Surg 2021 Jul 10;31(7):3040-3052. Epub 2021 May 10.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: This study aimed to investigate the weight-independent mechanism of sleeve gastrectomy on the relief of nonalcoholic fatty liver disease (NAFLD).

Methods: A total of 58 obese patients who had undergone sleeve gastrostomy (SG) were recruited. Plasma levels of indole-3-acetic acid (I3A), a metabolite from gut microbiota before and after SG were investigated. In addition, we had 78 C57BL/6J mice included in the study. High-fat diet (HFD) was used to induce obesity in mice. Sleeve gastrectomy (SG) was then performed. The liver of the mice was analyzed by HE and oil red staining to study lipid accumulation. Fluorescence-activated cell sorting (FACS) analysis was performed to study the phenotype of macrophages in the liver. The levels of I3A in serum, stool, and liver were tested by ELISA. Macrophages and hepatocytes were cultured in vitro and stimulated with I3A to study the effects on differentiation and proliferation/apoptosis.

Results: In human samples, I3A increased after SG and plasma I3A levels were positively correlated with liver CT values and negatively correlated with liver fat attenuation. In mice models, after surgery, the percentage of M2 macrophages significantly increased in the liver. Both oral gavage and in vitro stimulation of I3A could promote M2 differentiation and did not significantly affect the state of hepatocytes.

Conclusions: This study suggested that increased I3A from the intestine after SG could reduce the M1/M2 ratio in the liver and thus promote relief of NAFLD in obese individuals. Graphical Abstract.
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http://dx.doi.org/10.1007/s11695-021-05321-0DOI Listing
July 2021

Sex differences in the association between serum BDNF and cognitive impairment in schizophrenia patients using various antipsychotics.

J Psychiatr Res 2021 Jun 30;138:492-499. Epub 2021 Apr 30.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Background: Cognitive impairment is one of the core symptoms of schizophrenia patients. There are often various differences in the efficacy of different antipsychotics in the treatment of cognitive impairment by sex. The purpose of this study was to explore whether there are gender differences in the association between serum BDNF levels and cognitive performance in patients with schizophrenia taking different antipsychotics.

Methods: We used Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) to assess the cognitive function of three groups of schizophrenia patients (420 on clozapine, 183 on risperidone, 215 on typical antipsychotic drugs) and 467 healthy controls. Positive and Negative Syndrome Scale (PANSS) was used to assess schizophrenia symptoms of patients. Enzyme-Linked ImmunoSorbent Assay was used to measure serum brain-derived neurotrophic factor (BDNF) levels.

Results: Among the patients taking clozapine and typical antipsychotic drugs, the RBANS total score, immediate memory, attention, and delayed memory subscores in females were higher than those in males (all p < 0.05). The RBANS total score and the delayed memory subscores in female patients taking risperidone were higher than those in male patients (all p < 0.05). Significant correlation between BDNF and cognition only existed in male patients taking clozapine, male patients taking risperidone, and male and female patients taking typical antipsychotic drugs (all p < 0.05).

Conclusion: Regardless of antipsychotic effect, the cognitive function of female patients is better compared to that of male patients. For male patients, the association between BDNF and cognitive performance exists in each medication group. For female patients, this significant association was only shown in the typical antipsychotic group, but not in the clozapine and risperidone groups.
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http://dx.doi.org/10.1016/j.jpsychires.2021.04.026DOI Listing
June 2021

Expression and function of vasoactive intestinal peptide receptors in human lower esophageal sphincter.

Authors:
Tao Jia Ning Zhao

Ann Palliat Med 2021 Mar;10(3):3067-3077

The Eighth Department of General surgery, Handan Central Hospital, Handan, China.

Background: Vasoactive intestinal peptide (VIP) is an important neurotransmitter involved in the modulation of gastrointestinal function through the stimulation of VIP receptors. However, the expression of VPAC1R, VPAC2R and PAC1R in the human Lower esophageal sphincter (LES) has not been fully clarified. Therefore, the purpose of this study is to explore the expression of these receptors in the human Lower esophageal sphincter, the responses of the Lower esophageal sphincter to Vasoactive intestinal peptide, and the role of Vasoactive intestinal peptide receptors in the responses.

Methods: Sling and clasp fiber samples of LES were acquired from patients undergoing subtotal esophagectomy, while circular muscle bundles from the esophagus and gastric fundus were used as control groups. Western blotting and RT-PCR technology were performed to determine the expression of the three VIP receptor subtypes. The isometric tension responses of the muscle sample strips to Ro25-1553 and PG99-465, and the effect of electrical field stimulation (EFS) on the sling and clasp fibers were studied.

Results: We found that VPAC2R messenger RNA (mRNA) and protein were expressed in the sling and clasp fibers of human LES. However, no VPAC1R or PAC1R mRNA and protein expressions were found in the LES samples. The sling and clasp fibers of the LES produced significant concentration-dependent relaxation following exposure to Ro25-1553 and EFS could induce them to produce frequency-dependent relaxation. Furthermore, the relaxation responses of the LES were inhibited by PG99-465 and induced by EFS and Ro25-1553.

Conclusions: VPAC2R, but not VPAC1R or PAC1R, is expressed by the human LES. The relaxation responses of the LES generated by the VIP receptor agonist Ro25-1553 and EFS could be inhibited by the selective VPAC2 receptor antagonist PG99-465. VPAC2R may be important for the generation of relaxation and functional regulation of the LES.
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http://dx.doi.org/10.21037/apm-21-193DOI Listing
March 2021

Transcriptomics-based analysis of the mechanism by which Wang-Bi capsule alleviates joint destruction in rats with collagen-induced arthritis.

Chin Med 2021 Apr 12;16(1):31. Epub 2021 Apr 12.

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, 100700, China.

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease accompanied with joint destruction that often leads to disability. Wang-Bi capsule (WB), a traditional Chinese medicine-based herbs formula, has exhibited inhibition effect on joint destruction of collagen-induced arthritis (CIA) animal model in our previous study. But its molecular mechanisms are still obscure.

Methods: CIA rats were treated intragastrical with WB for eight weeks, and the effect of joints protection were evaluated by hematoxylin and eosin (H&E) staining, safranin O fast green staining, tartrate-resistant acid phosphatase (TRAP) staining and micro‑CT scanning analysis. The transcriptomic of tarsal joints were used to investigate how WB alleviated joint destruction.

Results: The histological examination of ankle joints showed WB alleviated both cartilage damage and bone destruction of CIA rats. This protective effect on joints were further evidenced by micro-CT analysis. The transcriptomic analysis showed that WB prominently changed 12 KEGG signaling pathways ("calcium signaling pathway", "cAMP signaling pathway", "cell adhesion molecules", "chemokine signaling pathway", "complement and coagulation cascades", "MAPK signaling pathway", "NF-kappa B signaling pathway", "osteoclast differentiation", "PI3K-Akt signaling pathway", "focal adhesion", "Gap junction" and "Rap1 signaling pathway") associated with bone or cartilage. Several genes (including Il6, Tnfsf11, Ffar2, Plg, Tnfrsf11b, Fgf4, Fpr1, Siglec1, Vegfd, Cldn1, Cxcl13, Chad, Arrb2, Fgf9, Egfr) regulating bone resorption, bone formation and cartilage development were identified by further analysis. Meanwhile, these differentially expressed genes were validated by real-time quantitative PCR.

Conclusions: Overall, the protective effect of WB treatment on joint were confirmed in CIA rats, and its basic molecular mechanisms may be associated with regulating some genes (including Il6, Tnfsf11, Ffar2 and Plg etc.) involved in bone resorption, bone formation and cartilage development.
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http://dx.doi.org/10.1186/s13020-021-00439-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042720PMC
April 2021

Corrigendum to Generalized anxiety disorder, depressive symptoms and sleep quality during COVID-19 outbreak in China: a web-based cross-sectional survey [Psychiatry Research, 288 (2020) 112954].

Psychiatry Res 2021 May 8;299:113803. Epub 2021 Apr 8.

The 6th Affiliated Hospital of Shenzhen University Health Science Center, Nanshan Hospital Affiliated to Shenzhen University, Shenzhen, 518052, People's Republic of China; Department of Rehabilitation Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, 518052, People's Republic of China. Electronic address:

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http://dx.doi.org/10.1016/j.psychres.2021.113803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026267PMC
May 2021

Research on obstacle avoidance algorithm for unmanned ground vehicle based on multi-sensor information fusion.

Math Biosci Eng 2021 01;18(2):1022-1039

School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou 510640, China.

With the wide application of unmanned ground vehicles (UGV) in a complex environment, the research on the obstacle avoidance system has gradually become an important research part in the field of the UGV system. Aiming at the complex working environment, a sensor detection system mounted on UGV is designed and the kinematic estimation model of UGV is studied. In order to meet the obstacle avoidance requirements of UGVs in a complex environment, a fuzzy neural network obstacle avoidance algorithm based on multi-sensor information fusion is designed in this paper. MATLAB is used to simulate the obstacle avoidance algorithm. By comparing and analyzing the simulation path of UGV's obstacle avoidance motion under the navigation control of fuzzy controller and fuzzy neural network algorithm, the superiority of the proposed fuzzy neural network algorithm was verified. Finally, the superiority and reliability of the obstacle avoidance algorithm are verified through the obstacle avoidance experiment on the UGV experimental platform.
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http://dx.doi.org/10.3934/mbe.2021055DOI Listing
January 2021

Establishment of a typing model for diffuse large B-cell lymphoma based on B-cell receptor repertoire sequencing.

BMC Cancer 2021 Mar 22;21(1):297. Epub 2021 Mar 22.

Department of Oncology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, No.354 Beima Road, Hongqiao District, Tianjin, 300120, China.

Background: The purpose of this study was to construct a new typing model for diffuse large B-cell lymphoma (DLBCL) patients based on the B-cell receptor (BCR) and explore its potential molecular mechanism.

Methods: BCR repertoire sequencing and whole-exome sequencing were performed on formalin-fixed paraffin-embedded samples from 12 DLBCL patients. Subsequently, a typing model was built with cluster analysis, and prognostic indicators between the two groups were compared to verify the typing model. Then, mutation and bioinformatics analyses were conducted to investigate the potential biomarkers of prognostic differences between the two groups.

Results: Based on BCR sequencing data, we divided patients into two clusters (cluster 1 and cluster 2); this classification differed from the traditional typing method (GCB and non-GCB), in which cluster 1 included some non-GCB patients. The progression-free survival (PFS), overall survival (OS), metastasis and Shannon diversity index of IGH V-J and survival after chemotherapy were significantly different (P < 0.05) between the two clusters, but no statistical significance was found between the GCB and non-GCB groups. The mutation status of 248 genes was significantly different between cluster 1 and cluster 2. Among them, FTSJ3, MAGED2, and ODF3L2 were the specific mutated genes in all patients in cluster 2, and these genes could be considered critical to the different prognoses of the two clusters of DLBCL patients.

Conclusion: We constructed a new typing model of DLBCL based on BCR repertoire sequencing that can better predict the survival time after chemotherapy. FTSJ3, MAGED2, and ODF3L2 may represent key genes for the difference in prognosis between the two clusters.
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http://dx.doi.org/10.1186/s12885-021-08015-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986026PMC
March 2021

Transparent Super-Repellent Surfaces with Low Haze and High Jet Impact Resistance.

ACS Appl Mater Interfaces 2021 Mar 9;13(11):13813-13821. Epub 2021 Mar 9.

Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Transparent superhydrophobic surfaces are of vital significance for rising applications in optoelectronics, outdoor displays, building windows, and so on. However, facile fabrication of surfaces combining stable superhydrophobicity and high transparency with particularly low haze remains a challenge. Here, we demonstrate a nonfluorinated hierarchical surface, simply prepared by sequential spraying of a primer of poly(ethylene--acrylic acid) (EAA) and silica nanoparticles (SiO). The resultant surface shows remarkable liquid repellency (e.g., an apparent contact angle of >160° and a sliding angle of <2° for honey) and high transparency (a transmittance of ∼91% and a haze of ∼6%). Especially, flexible EAA adhesive enables the surface to resist water impinging (up to ∼15.0 m s, higher than the terminal velocities of raindrops) and mechanical damaging. This super-repellent surface also presents excellent UV and chemical stability, sustaining a superhydrophobic state upon UVA exposure for 60 days and acidic corrosion or oil contamination for 7 days. With multirobustness and scalability, our coatings show great potential in related fields.
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http://dx.doi.org/10.1021/acsami.0c23055DOI Listing
March 2021

Structurally diverse alkaloids from Buxus sempervirens with cardioprotective activity.

Bioorg Chem 2021 Apr 20;109:104753. Epub 2021 Feb 20.

Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China. Electronic address:

Extensive phytochemical study of the methanol extract of twigs and leaves of Buxus sempervirens resulted in the identification of 17 Buxus alkaloids, including 12 new ones, namely buxusemines A-L (1-12). Their structures were delineated by detailed analysis of the HRESIMS and NMR data, as well as quantum chemical NMR calculations. Buxusemine A (1) represents the second Buxus alkaloid with a unique spiro[4.6]undecatriene moiety, buxusemines B-C (2-3) are a rarely occurring class of Buxus alkaloids featured with an additional five-membered ring through the ether or lactone linkage between C-10 and C-23, and buxusemines D-F (4-6) are another rare type of Buxus alkaloids with an epoxy motif. In the assessment of their bioactivities, buxusemine F (6) and buxanoldine (17) displayed more potent protective effects than the positive control cyclovirobuxinum D in the doxorubicin-induced cardiac injury model.
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http://dx.doi.org/10.1016/j.bioorg.2021.104753DOI Listing
April 2021

Exogenous phytohormone application and transcriptome analysis of Mikania micrantha provides insights for a potential control strategy.

Genomics 2021 May 18;113(3):964-975. Epub 2021 Feb 18.

Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Electronic address:

Effective and complete control of the invasive weed Mikania micrantha is required to avoid increasing damages. We exogenously applied indole 3-acetic acid (IAA), gibberellin (GA), and N-(2-Chloro-4-pyridyl)-N'-phenylurea (CPPU), and their combinations i.e. IAA + CPPU (IC), GA + CPPU (GC), and GA + IAA + CPPU (GIC), at 5, 10, 25, 50, and 75 ppm against distilled water as a control (CK), to examine their effects on the weed. The increasing concentrations of these hormones when applied alone or in combination were fatal to M. micrantha and led towards the death of inflorescences and/or florets. CPPU and GIC were found as the most effective phytohormones. Transcriptome analysis revealed differential regulation of genes in auxin, cytokinin, gibberellin and abscisic acid signaling pathways, suggesting their role in the prohibition of axillary bud differentiation. Collectively, CPPU and GIC at a high concentration (75 ppm) could be used as a control measure to protect forests and other lands from the invasion of M. micrantha.
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http://dx.doi.org/10.1016/j.ygeno.2021.02.008DOI Listing
May 2021

Photoinduced Spontaneous Formation of a Reduced Graphene Oxide-Enwrapped Cu-CuO Nanocomposite for Solar Hydrogen Evolution.

ACS Appl Mater Interfaces 2021 Mar 17;13(8):9838-9845. Epub 2021 Feb 17.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China.

The fast recombination of photogenerated charge carriers and poor stability have impeded the application of many narrow band gap semiconductors with otherwise excellent photocatalytic performance. A metal-semiconductor Schottky junction is a promising strategy to accelerate charge separation and enhance catalytic efficiency. However, the preparation of these structures often involves intricate processes and harsh conditions, which will inevitably destroy the electronic structures of the semiconductors and ruin their original properties in practical applications. In this study, a reduced graphene oxide (RGO)-enwrapped Cu-CuO nanocomposite ([email protected]) spontaneously evolved from an aqueous alcoholic solution containing cupric ions and graphene oxide (GO) under simulated sunlight irradiation. During this process, GO reduction and Cu-CuO nanoparticles growth occurred simultaneously in conjunction with RGO encapsulation. Benefiting from the superior intrinsic semiconductor characteristic retention under mild reaction conditions, strong component interactions, and efficient interfacial charge transfer, the distinctive [email protected] nanocomposite supplied multiple channels for rapid electron transfer to substantially enhance the charge carrier separation efficiency and provide perfect chemical protection to effectively prevent CuO photocorrosion. This product also greatly suppressed self-aggregation to decrease the size of nanoparticles. Based on these merits, the [email protected] nanocomposite offered promising advances in photoelectrochemical and photocatalytic H evolution. This work provides an innovative photoinduced strategy for constructing an RGO-enwrapped semiconductor nanocomposite with efficient charge transfer interfaces while providing novel insights for the efficient solar energy utilization.
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http://dx.doi.org/10.1021/acsami.0c20636DOI Listing
March 2021

Ratiometric determination of Cr(VI) based on a dual-emission fluorescent nanoprobe using carbon quantum dots and a smartphone app.

Mikrochim Acta 2021 Feb 16;188(3):89. Epub 2021 Feb 16.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin, 300457, China.

A simple dual-colour fluorescent nanoprobe has been designed composed of blue and yellow emission carbon quantum dots (CQDs). This system is inexpensive and easy to operate and was successfully employed for on-site measurements based on a smartphone app. The designed nanoprobe exhibited increased selectivity for Cr(VI), leading to a double stable response of the two CQDs. The dual-emission nanoprobe showed blue-violet fluorescence upon UV irradiation, and the fluorescent emission peaks were located at 418 nm and 552 nm. The blue light emission of CQDs was quenched with increasing Cr(VI) concentration due to the inner filter effect, whereas the yellow light emission was enhanced due to the aggregation-induced emission effect. The different responses of the dual emissions to Cr(VI) resulted in a fluorescent colour variation, thus enabling facile macroscopic visualization. With a smartphone, the change in the fluorescence colour could be observed more apparently than that of a single fluorescence nanoprobe, and the response increased linearly so that the nanoprobe could be applied to instantaneous measurements. Furthermore, the dual-emission nanoprobe was successfully employed for analysing food and water samples. Accurate concentrations were obtained by constructing a calibration plot using a fluorescence spectrometer and a smartphone app; the recoveries were 81.6% to 107.7%, and the relative standard deviation was below 3.6%. Therefore, this smartphone-integrated dual-emission detection system is promising as a new portable method for the on-site measurement of Cr(VI) ions. * Y-CQDs: yellow emission carbon quantum dots. B-CQDs: blue emission carbon quantum dots. B/Y-CQDs: a mixture of B-CQDs and Y-CQDs.
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http://dx.doi.org/10.1007/s00604-021-04747-8DOI Listing
February 2021

Protein manipulation using single copies of short peptide tags in cultured cells and in .

Development 2021 Mar 16;148(6). Epub 2021 Mar 16.

Institute for Biology I, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany

Cellular development and function rely on highly dynamic molecular interactions among proteins distributed in all cell compartments. Analysis of these interactions has been one of the main topics in cellular and developmental research, and has been mostly achieved by the manipulation of proteins of interest (POIs) at the genetic level. Although genetic strategies have significantly contributed to our current understanding, targeting specific interactions of POIs in a time- and space-controlled manner or analysing the role of POIs in dynamic cellular processes, such as cell migration or cell division, would benefit from more-direct approaches. The recent development of specific protein binders, which can be expressed and function intracellularly, along with advancement in synthetic biology, have contributed to the creation of a new toolbox for direct protein manipulations. Here, we have selected a number of short-tag epitopes for which protein binders from different scaffolds have been generated and showed that single copies of these tags allowed efficient POI binding and manipulation in living cells. Using , we also find that single short tags can be used for POI manipulation .
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http://dx.doi.org/10.1242/dev.191700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990863PMC
March 2021

Efficacy and safety of acupuncture on childhood attention deficit hyperactivity disorder: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(5):e23953

The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University), No. 98 Fenghuang Road, Huichuan District, Zunyi, Guizhou, China.

Introduction: The purpose of this paper is to evaluate the efficacy and safety of acupuncture in the treatment of childhood attention deficit hyperactivity disorder (ADHD).

Methods And Analysis: We will electronically search PubMed, Medline, Embase, Web of Science, the Cochrane Central Register of Controlled Trial, China National Knowledge Infrastructure, China Biomedical Literature Database, China Science Journal Database, and Wan-fang Database from their inception. Also, we will manually retrieve other resources, including reference lists of identified publications, conference articles, and grey literature. The clinical randomized controlled trials or quasi-randomized controlled trials related to acupuncture treating pediatric ADHD will be included in the study. The language is limited to Chinese and English. Research selection, data extraction, and research quality assessment will be independently completed by 2 researchers. Data were synthesized by using a fixed effect model or random effect model depend on the heterogeneity test. The scores of Revised Conners' Parent Rating Scale (CPRS-R), Conners Teacher Rating Scale (CTRS-R), and Child Behavior Checklist (CBCL) will be the primary outcomes. Besides, the scores of the Conners Continuous Performance Test, Internal Restlessness Scale, and Behavior Assessment System for Children (BASC), and the possible adverse events will also be assessed as secondary outcomes. RevMan V.5.3 statistical software will be used for meta-analysis, and the level of evidence will be assessed by Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Continuous data will be expressed in the form of weighted mean difference or standardized mean difference with 95% confidence intervals (CIs), while dichotomous data will be expressed in the form of relative risk with 95% CIs.

Ethics And Dissemination: The protocol of this systematic review (SR) does not require ethical approval because it does not involve humans. We will publish this article in peer-reviewed journals and presented at relevant conferences.

Systematic Review Registration: OSF Registries, DOI: 10.17605/OSF.IO/XVYP9 (https://osf.io/xvyp9).
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http://dx.doi.org/10.1097/MD.0000000000023953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870224PMC
February 2021

Insight into the Influence of the Graphite Layer and Cobalt Crystalline on a ZIF-67-Derived Catalyst for Fischer-Tropsch Synthesis.

ACS Appl Mater Interfaces 2021 Mar 16;13(8):9885-9896. Epub 2021 Feb 16.

Key Lab for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.

Due to the special framework structure, ZIF-67 is a promising material as the precursor to prepare the [email protected] catalysts with high cobalt loading and superior cobalt dispersion. Unfortunately, these [email protected] catalysts exhibit not only unsatisfied activity but also high CH selectivity. This limited its further application due to the lack of in-depth analysis of the reasons behind it. In this work, the [email protected] catalysts were prepared by pyrolyzing the ZIF-67 precursor at different temperatures. A series of characterizations were conducted to explore the behavior of the graphite carbon coated on cobalt species, realizing that the role of active Co sites on these [email protected] catalysts was restricted by the graphite carbon layer since it suppressed the adsorption and activation of syngas on Co sites. TEOS was introduced to suppress the aggregation of cobalt species and more active sites were exposed after the graphite carbon layer was eliminated. As a result, the FTS performance was greatly improved by a factor of 5. The effect of O concentration on the microcrystalline size of Co and the reconfinement effect of SiO were investigated. The model catalyst was prepared and the key factors determining CH selectivity of the ZIF-67-derived [email protected] catalyst were revealed. This provides a good basis for rational designing ZIF-67-derived Co-based FTS catalysts.
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http://dx.doi.org/10.1021/acsami.0c20888DOI Listing
March 2021

A novel sweetpotato RING-H2 type E3 ubiquitin ligase gene IbATL38 enhances salt tolerance in transgenic Arabidopsis.

Plant Sci 2021 Mar 6;304:110802. Epub 2021 Jan 6.

Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address:

Arabidopsis Toxicos en Levadura (ATL) proteins compose a subfamily of E3 ubiquitin ligases and play major roles in regulating plant growth, cold, drought, oxidative stresses response and pathogen defense in plants. However, the role in enhancing salt tolerance has not been reported to date. Here, we cloned a novel RING-H2 type E3 ubiquitin ligase gene, named IbATL38, from sweetpotato cultivar Lushu 3. This gene was highly expressed in the leaves of sweetpotato and strongly induced by NaCl and abscisic acid (ABA). This IbATL38 was localized to nucleus and plasm membrane and possessed E3 ubiquitin ligase activity. Overexpression of IbATL38 in Arabidopsis significantly enhanced salt tolerance, along with inducible expression of a series of stress-responsive genes and prominently decrease of HO content. These results suggest that IbATL38 as a novel E3 ubiquitin ligase may play an important role in salt stress response.
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http://dx.doi.org/10.1016/j.plantsci.2020.110802DOI Listing
March 2021

Determination of Factors Affecting Dengue Occurrence in Representative Areas of China: A Principal Component Regression Analysis.

Front Public Health 2020 18;8:603872. Epub 2021 Jan 18.

State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, WHO Collaborating Centre for Vector Surveillance and Management, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Determination of the key factors affecting dengue occurrence is of significant importance for the successful response to its outbreak. Yunnan and Guangdong Provinces in China are hotspots of dengue outbreak during recent years. However, few studies focused on the drive of multi-dimensional factors on dengue occurrence failing to consider the possible multicollinearity of the studied factors, which may bias the results. In this study, multiple linear regression analysis was utilized to explore the effect of multicollinearity among dengue occurrences and related natural and social factors. A principal component regression (PCR) analysis was utilized to determine the key dengue-driven factors in Guangzhou city of Guangdong Province and Xishuangbanna prefecture of Yunnan Province, respectively. The effect of multicollinearity existed in both Guangzhou city and Xishuangbanna prefecture, respectively. PCR model revealed that the top three contributing factors to dengue occurrence in Guangzhou were Breteau Index (BI) (positive correlation), the number of imported dengue cases lagged by 1 month (positive correlation), and monthly average of maximum temperature lagged by 1 month (negative correlation). In contrast, the top three factors contributing to dengue occurrence in Xishuangbanna included monthly average of minimum temperature lagged by 1 month (positive correlation), monthly average of maximum temperature (positive correlation), monthly average of relative humidity (positive correlation), respectively. Meteorological factors presented stronger impacts on dengue occurrence in Xishuangbanna, Yunnan, while BI and the number of imported cases lagged by 1 month played important roles on dengue transmission in Guangzhou, Guangdong. Our findings could help to facilitate the formulation of tailored dengue response mechanism in representative areas of China in the future.
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http://dx.doi.org/10.3389/fpubh.2020.603872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848178PMC
May 2021

An epithelial-mesenchymal transition-related long non-coding RNA signature to predict overall survival and immune microenvironment in kidney renal clear cell carcinoma.

Bioengineered 2021 Dec;12(1):555-564

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, China.

Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from the Cancer Genome Atlas (TCGA-KIRC). We first determined 1377 EMT-related lncRNAs, lncRNA AL035661.1 with the largest correlation coefficient and the target gene was (cor = 0.843; = 1.37E-146). Meanwhile, we found an AUC of 0.758 in our signature and we predicted the AUC values of the patients' 1, 2, 3-year survival rate as 0.768, 0.749, and 0.762 in TCGA cohort, respectively. Multivariate COX analysis was performed to determine if risk score was an independent prognostic predictor of OS. The results indicated that our risk score can be an independent predictor for OS (Univariate: HR = 1.350, 95% CI = 1.276-1.428, < 0.001; Multivariate: HR = 1.295, 95% CI = 1.201-1.396, < 0.001). We identified novel EMT-related lncRNAs markers for ccRCC prognosis. The underlying mechanism between EMT-related lncRNAs in ccRCC and tumor immunity is still unclear and requires further study.
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http://dx.doi.org/10.1080/21655979.2021.1880718DOI Listing
December 2021

Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide.

Sci Rep 2021 Jan 27;11(1):2365. Epub 2021 Jan 27.

College of Life Sciences, Nanjing Normal University, No.1 Wenyuan Road, Qixia District, Nanjing, 210023, People's Republic of China.

Shiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis and regulatory pathways are still unclear. In this study, nitric oxide, as a downstream signal molecule of hydrogen peroxide, regulates the biosynthesis of perylenequinones. Exogenous addition of 0.01 mM sodium nitroprusside (nitric oxide donor) can promote perylenequinones production by 156% compared with the control. Further research found that hydrogen peroxide and nitric oxide increased the transcriptional level of the biosynthetic genes of hypocrellin A. The results showed that nitric oxide is involved in the biosynthesis and regulation of perylenequinones in Shiraia bambusicola as a signal molecule. In the future, the yield of perylenequinones can be increased by adding exogenous nitric oxide in fermentation.
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http://dx.doi.org/10.1038/s41598-021-81990-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840948PMC
January 2021

Neuromodulatory Effect of Sensorimotor Network Functional Connectivity of Temporal Three-Needle Therapy for Ischemic Stroke Patients with Motor Dysfunction: Study Protocol for a Randomized, Patient-Assessor Blind, Controlled, Neuroimaging Trial.

Evid Based Complement Alternat Med 2021 4;2021:8820324. Epub 2021 Jan 4.

Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.

Background: The clinical efficacy of temporal three-needle therapy for stroke dysfunction has been previously demonstrated in China. However, the central mechanism of temporal three-needle therapy remains unclear. Temporal three-needle projects the sensory cortex and the motor cortex, which may impact the cortex function. Current studies seldom focus on it. Hence, according to the "scalp-cortex corresponding theory," the underlying mechanism of temporal three-needle remains a domain for further research.

Methods: This trial is designed to provide objective and visual evidence for the neuromodulatory effect and neuroimaging mechanism of temporal three-needle therapy for stroke patients. This ongoing study is a prospective, randomized, controlled, patient-assessor blind, single-center, neuroimaging trial involving two-parallel patient groups and a healthy control group. Forty eligible patients will be recruited from Shenzhen Nanshan District People's Hospital and randomized into either the experimental group or the control group. Twenty healthy volunteers will be recruited in the healthy control group and undergo baseline magnetic resonance imaging scans without any intervention. Patients in the control group will receive acupuncture at Dingnieqianxiexian (MS6), in addition to basic medicine and rehabilitative treatments. Patients in the experimental group will receive temporal three-needle therapy plus basic medicine and rehabilitative treatments 5 days per week, 10 sessions over two consecutive weeks. The primary outcome is resting-state functional connectivity, and the secondary outcomes are regional homogeneity, amplitude of low-frequency fluctuations, Fugl-Meyer assessment of the upper limb, and modified Barthel Index. All outcome measures will be assessed at baseline and after 2 weeks of intervention. . The results will explore the neuromodulatory effects and illustrate the central mechanism of temporal three-needle treatment from the network-level viewpoint of sensorimotor network functional plasticity and promote widespread application in real-world practice. This trial was registered at Chinese Clinical Trial Registry on 14 March 2018 with ChiCTR1800015209.
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http://dx.doi.org/10.1155/2021/8820324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801060PMC
January 2021

Characteristics and Survival Outcomes of Second Primary Cancers in Long-term Retinoblastoma Survivors.

Asia Pac J Ophthalmol (Phila) 2021 Jan 19. Epub 2021 Jan 19.

Orbital Oncology & Ophthalmic Plastic Surgery, Department of Plastic Surgery Department of Biostatistics Department of Neuroradiology, The University of Texas MD Anderson Cancer Center, Houston, TX.

Purpose: Retinoblastoma (RB) is the most common intraocular cancer and is associated with lifelong risks of developing a second primary cancer, especially in patients with hereditary RB and/or childhood exposure to radiotherapy.

Methods: The study included all consecutive patients with a history of RB treated for a second primary cancer during 1994-2018. Patient demographics and characteristics of the primary RB and second primary cancer were examined. The associations among radiation or chemotherapy exposure as a treatment for RB, unilateral versus bilateral status, types and multiplicity of second primary cancers, and survival after diagnosis of second primary cancer were analyzed.

Results: A wide spectrum of second primary cancer types was identified from 62 eligible patients (30 males and 32 females), including sarcoma, breast cancer, various skin cancers, gastrointestinal and genitourinary cancers, and endocrine cancers. Of all patients who had second primary cancers, 40 patients (65%) had bilateral RB and 17 patients (27%) had unilateral RB. Thirty-five patients (56%) who developed second primary cancers received radiation therapy during childhood as the treatment of RB, and 17 patients (27%) received chemotherapy for the treatment of RB. The 5-year and 10-year survival rates for RB patients diagnosed with a second primary cancer were 54.0% and 36.0%, respectively. The median age of onset of second primary cancer among RB survivors was 36.6 years.

Conclusions: In contrast to previous studies, we found a broader spectrum of second primary cancer types. All RB survivors, regardless of unilateral or bilateral status, should undergo strict cancer surveillance particularly as they approach the fourth decade of life.
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http://dx.doi.org/10.1097/APO.0000000000000361DOI Listing
January 2021

Effects of an industrial passive assistive exoskeleton on muscle activity, oxygen consumption and subjective responses during lifting tasks.

PLoS One 2021 20;16(1):e0245629. Epub 2021 Jan 20.

School of Mechanical and Automotive Engineering, South China University of Technology, Guangzhou, China.

The purpose of this study was to evaluate the effects of an industrial passive assisted exoskeleton (IPAE) with simulated lifting tasks on muscle activity, oxygen consumption, perceived level of exertion, local perceived pressure, and systemic usability. Eight workers were required to complete two lifting tasks with and without the IPAE, that were single lifting tasks (repeated 5 times) and 15 min repeated lifting tasks respectively. Both of the tasks required subjects to remove a toolbox from the ground to the waist height. The test results showed that IPAE significantly reduced the muscle activity of the lumbar erector spinae, thoracic erector spinae, middle deltoid and labrum-biceps muscles; the reduction effect during the 15 min lifting task was reached 21%, 12%, 32% and 38% respectively. The exoskeleton did not cause significant differences in oxygen consumption and the perceived level of exertion, but local perceived pressure on the shoulders, thighs, wrists, and waist of the subjects could be produced. 50% of the subjects rated the usability of the equipment as acceptable. The results illustrate the good potential of the exoskeleton to reduce the muscle activity of the low back and upper arms. However, there is still a concern for the obvious contact pressure.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245629PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816984PMC
June 2021

Increased hydrophobicity of CRYGD p.(Ala159ProfsTer9): Suspected cause of congenital cataracts in a large Chinese family.

Mol Genet Genomic Med 2020 10 23;8(10):e1436. Epub 2020 Jul 23.

NHC Key Laboratory of Reproductive Health and Medical Genetics, Liaoning Research Institute of Family Planning, The Affiliated Reproductive Hospital of China Medical University, Shenyang, China.

Objective: This study aimed to identify the disease-causing mutation of congenital cataract disease in a large northeastern Chinese family.

Materials And Methods: The subjects' peripheral blood was collected, their genomic DNA was extracted, mutation screening of candidate genes was performed using polymerase chain reaction, and the amplified products were sequenced. Recombinant C-terminal enhanced green fluorescent protein-tagged wild-type or mutant CRYGD was expressed in HEK293T cells, and the expression pattern was observed under a fluorescence microscope. The CRYGD protein mutation was analyzed via bioinformatics analysis.

Results: c.475delG, a novel frameshift mutation in CRYGD, was identified in the affected family members. This mutation causes premature termination of the polypeptide, resulting in truncated p.(Ala159ProfsTer9). According to the bioinformatics analysis results, compared with wild-type CRYGD, p.(Ala159ProfsTer9) exhibits significantly decreased hydrophilicity. Fluorescence microscopy revealed that p.(Ala159ProfsTer9) aggregates in the cell in the form of granular deposits.

Conclusion: In this study, the novel frameshift mutation c.475delG, p.(Ala159ProfsTer9) in CRYGD was identified to cause congenital cataracts in a large Chinese family; increased hydrophobicity of p.(Ala159ProfsTer9) protein may be the underlying mechanism.
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http://dx.doi.org/10.1002/mgg3.1436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549552PMC
October 2020

The lipoprotein NlpD in responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity.

Virulence 2021 12;12(1):415-429

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University , Tianjin, China.

, an emerging opportunistic pathogen, is implicated in severe foodborne outbreak infections in premature and full-term infants. Generally, acid tolerance is vital for the pathogenesis of foodborne pathogens; however, its role in virulence remains largely unknown. To screen out acid-tolerance determinants from transposon mutants, anovel counterselection method using gentamicin and acid was developed. Using the counterselection method and growth assay, we screened several acid-sensitive mutants and found that  encodes an acid-resistance factor in .  Compared to the wild-type strain, the mutant exhibited attenuated virulence in a rat model. Using macrophage THP-1 cells and a pH probe, we verified that enables bacteria to resist macrophages by resisting acidification. Finally, we confirmed that maintains membrane integrity in acid using propidium iodide permeabilization assays via flow cytometry. Our results confirm that is a novel virulence factor that permits to survive under acid stress conditions. Considering that NlpD is a conserved lipoprotein located in the bacterial outer membrane, NlpD could be used as a target for drug development.
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http://dx.doi.org/10.1080/21505594.2020.1870336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834084PMC
December 2021

An Extremely Stretchable and Self-Healable Supramolecular Polymer Network.

ACS Appl Mater Interfaces 2021 Jan 12;13(3):4499-4507. Epub 2021 Jan 12.

Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The construction of a single polymer network with extreme stretchability, relatively high mechanical strength, and fast and facile autonomous room-temperature self-healing capability still remains a challenge. Herein, supramolecular polymer networks are fabricated by synergistically incorporating metal-ligand and hydrogen bonds in poly(propylene glycol) (PPG). The representative specimen, PPG-Im-MDA-1.5-0.25-Cu, shows a combination of notable mechanical properties involving an extreme stretching ratio of 346 ± 14× and a Young's modulus of 2.10 ± 0.14 MPa, which are superior to the previously reported extremely stretchable polymeric materials. Notably, the destroyed specimen can fully recover mechanical performances within 1 h. The tunability of mechanical properties and self-healing capability has been actualized by merely tailoring the content of a chain extender. The application of the as-prepared supramolecular PPG network in constructing a flexible and self-healable conductor has been demonstrated. This strategy provides some insights for preparing extremely stretchable and self-healable polymeric materials.
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http://dx.doi.org/10.1021/acsami.0c19560DOI Listing
January 2021