Publications by authors named "Ning Zhang"

3,211 Publications

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A breakthrough in liver regeneration for treatment of liver cancer.

Cancer Biol Med 2021 Aug;18(3)

Translational Cancer Research Center, Peking University, Peking University First Hospital, Beijing 100034, China.

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http://dx.doi.org/10.20892/j.issn.2095-3941.2021.0293DOI Listing
August 2021

Differential dynamics of bone graft transplantation and mesenchymal stem cell therapy during bone defect healing in a murine critical size defect.

J Orthop Translat 2022 Sep 4;36:64-74. Epub 2022 Aug 4.

Department of Orthopaedic Surgery, Stanford University, Stanford, CA, USA.

Background: A critical size bone defect is a clinical scenario in which bone is lost or excised due to trauma, infection, tumor, or other causes, and cannot completely heal spontaneously. The most common treatment for this condition is autologous bone grafting to the defect site. However, autologous bone graft is often insufficient in quantity or quality for transplantation to these large defects. Recently, tissue engineering methods using mesenchymal stem cells (MSCs) have been proposed as an alternative treatment. However, the underlying biological principles and optimal techniques for tissue regeneration of bone using stem cell therapy have not been completely elucidated.

Methods: In this study, we compare the early cellular dynamics of healing between bone graft transplantation and MSC therapy in a murine chronic femoral critical-size bone defect. We employ high-dimensional mass cytometry to provide a comprehensive view of the differences in cell composition, stem cell functionality, and immunomodulatory activity between these two treatment methods one week after transplantation.

Results: We reveal distinct cell compositions among tissues from bone defect sites compared with original bone graft, show active recruitment of MSCs to the bone defect sites, and demonstrate the phenotypic diversity of macrophages and T cells in each group that may affect the clinical outcome.

Conclusion: Our results provide critical data and future directions on the use of MSCs for treating critical size defects to regenerate bone. This study showed systematic comparisons of the cellular and immunomodulatory profiles among different interventions to improve the healing of the critical-size bone defect. The results provided potential strategies for designing robust therapeutic interventions for the unmet clinical need of treating critical-size bone defects.
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http://dx.doi.org/10.1016/j.jot.2022.05.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357712PMC
September 2022

Development of Risk Prediction Model for Muscular Calf Vein Thrombosis with Acute Exacerbation of Chronic Obstructive Pulmonary Disease.

Int J Gen Med 2022 10;15:6549-6560. Epub 2022 Aug 10.

Department of Respiratory and Critical Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.

Purpose: This study aims to establish a risk prediction model for muscular calf vein thrombosis (MCVT) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).

Methods: The research sample consisted of 248 patients with AECOPD and all of them underwent vascular ultrasounds of both lower limbs in this retrospective study. Univariate analysis and multivariate logistic regression analysis were conducted on factors with significant group differences to screen for the independent risk factors of MCVT. A nomogram to predict the risk of MCVT was constructed and validated with bootstrap resampling.

Results: According to the exclusion criteria, 240 patients were included for analysis, divided into the MCVT group (n = 81) and the non-MCVT group (n = 159). Multivariate logistic regression analyses showed that hypertension, elevated MPV, reduced albumin (ALB), elevated D-dimer and bed rest ≥3 days were independent risk factors for MCVT in AECOPD. A nomogram model for predicting AECOPD with MCVT was established based on them. The area under the curve (AUC) of receiver operating characteristic (ROC) curve for the prediction model and the simplified Wells score was 0.784 (95% CI: 0.722-0.847) and 0.659 (95% CI: 0.583-0.735), respectively. The cut-off value and Youden index of prediction model were 0.248 and 0.454, respectively. At the same time, the sensitivity, specificity, positive predictive value, and negative predictive value of the prediction model were 85.9%, 59.5%, 84.6%, and 77.4%, respectively. The sensitivity and specificity of the simplified Wells score were 67.9% and 56.3%, respectively. Validation by the use of bootstrap resampling revealed optimal discrimination and calibration, and the decision analysis curve (DAC) suggested that this prediction model involved high clinical practicability.

Conclusion: We developed a nomogram that can predict the risk of MCVT for AECOPD patients. This model has the potential to assist clinicians in making treatment recommendations and formulating corresponding prevention measures.
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http://dx.doi.org/10.2147/IJGM.S374777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375990PMC
August 2022

Ribosomal protein L22-like1 (RPL22L1) mediates sorafenib sensitivity via ERK in hepatocellular carcinoma.

Cell Death Discov 2022 Aug 17;8(1):365. Epub 2022 Aug 17.

Laboratory of Medical Genetics, Harbin Medical University, Harbin, China.

Precision medicine in hepatocellular carcinoma (HCC) relies on validated biomarkers that help subgroup patients for targeted treatment. Here, we identified a novel candidate oncogene, ribosomal protein L22-like1 (RPL22L1), which was markedly elevated in HCC, contributed to HCC malignancy and adverse patient survival. Functional studies indicated RPL22L1 overexpression accelerated cell proliferation, migration, invasion and sorafenib resistance. Mechanism studies revealed that RPL22L1 activated ERK to induce atypical epithelial-to-mesenchymal transition (EMT) progress. Importantly, the ERK inhibitor (ERKi) could potentiate sorafenib efficiency in RPL22L1-high HCC cells. In summary, these data uncover RPL22L1 is a potential marker to guide precision therapy for utilizing ERKi to enhance the sorafenib efficacy in RPL22L1-high HCC patients.
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http://dx.doi.org/10.1038/s41420-022-01153-8DOI Listing
August 2022

Dimeric-(-)-epigallocatechin-3-gallate inhibits the proliferation of lung cancer cells by inhibiting the EGFR signaling pathway.

Chem Biol Interact 2022 Aug 12:110084. Epub 2022 Aug 12.

Key Laboratory of Pu-erh Tea Science, Ministry of Education, College of Science, Yunnan Agricultural University, Kunming, 650201, China. Electronic address:

Non-small cell lung cancer (NSCLC) is one of the most general malignant tumors. The overexpression of epidermal growth factor receptor (EGFR) is a common marker in NSCLC, and it plays an important role in the proliferation, invasion, and metastasis of cancer cells. At present, drugs developed with EGFR as a target suffer from drug resistance, so it is necessary to study new compounds for the treatment of NSCLC. The active substance in green tea is EGCG, which has anti-cancer effects. In this study, we synthesized dimeric-(-)-epigallocatechin-3-gallate (prodelphinidin B-4-3,3‴-di-O-gallate, PBOG), and explored the effect of PBOG on lung cancer cells. PBOG can inhibit the proliferation and migration of NCI-H1975 cells, promote cell apoptosis, and inhibit cell cycle progression. In addition, PBOG can bind to the EGFR ectodomain protein and change the secondary structure of the protein. At the same time, PBOG decreases the expression of EGFR and downstream protein phosphorylation. Animal experiments confirmed that PBOG can inhibit tumor growth by inhibiting EGFR phosphorylation. Collectively, our study results show that PBOG may induce a decrease in intracellular phosphorylated EGFR expression by binding to the EGFR ectodomain protein, thereby inducing apoptosis and inhibiting cell cycle progression, thus providing a new strategy to treat lung cancer.
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http://dx.doi.org/10.1016/j.cbi.2022.110084DOI Listing
August 2022

Genome-Wide 3'-UTR Single Nucleotide Polymorphism Association Study Identifies Significant Prostate Cancer Risk-Associated Functional Loci at 8p21.2 in Chinese Population.

Adv Sci (Weinh) 2022 08 17;9(23):e2201420. Epub 2022 Jun 17.

Division of Urology, Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.

MicroRNAs (miRNAs) are involved in the regulation of gene expression via incomplete base pairing to sequence motifs at the three prime untranslated regions (3'-UTRs) of mRNAs and play critical roles in the etiology of cancers. Single nucleotide polymorphisms (SNPs) in the 3'-UTR miRNA-binding regions may influence the miRNA affinity. However, this biological mechanism in prostate cancer (PCa) remains unclear. Here, a three-stage genome-wide association study of 3'-UTR SNPs (n=33 117) is performed in 5515 Chinese men. Three genome-wide significant variants are discovered at 8p21.2 (rs1567669, rs4872176, and rs4872177), which are all located in a linkage disequilibrium region of the NKX3-1 gene. Phenome-wide association analysis using the FinnGen data reveals a specific association of rs1567669 with PCa over 2,264 disease endpoints. Expression quantitative trait locus analyses based on both Chinese PCa cohort and the GTEx database show that risk alleles of these SNPs are significantly associated with low expression of NKX3-1. Based on the MirSNP database, dual-luciferase reporter assays show that risk alleles of these SNPs downregulate the expression of NKX3-1 via increased miRNA binding. These results indicate that the SNPs at the 3'-UTR of NKX3-1 significantly downregulate NKX3-1 expression by influencing the affinity of miRNA and increase the PCa risk.
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http://dx.doi.org/10.1002/advs.202201420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9376745PMC
August 2022

Distinct host immune responses in recurrent vulvovaginal candidiasis and vulvovaginal candidiasis.

Front Immunol 2022 28;13:959740. Epub 2022 Jul 28.

Laboratory of Medical Mycology, Jining No.1 People's Hospital, Jining, China.

Recurrent vulvovaginal candidiasis (RVVC) and vulvovaginal candidiasis (RVVC) are one of the most common gynecological infections, primarily caused by species. Although risk factors of RVVC and VVC have been identified in many studies, antifungal immunological mechanisms are still not fully understood. We performed a 1-year prospective study in a local hospital to monitor 98 patients clinically diagnosed with gynecological infection. The results showed that 20.41% (20/98) are with RVVC, and 79.59% (78/98) patients have VVC. accounts for 90% and 96.1% of all strains isolated collected from RVVC and VVC patients, respectively. Antifungal susceptibility testing showed no significant difference in species between RVVC and VVC patients. However, the serum levels of IFN-γ, TNF-α, and IL-17F in the RVVC group were significantly lower than those of the VVC group, while IL-4, IL-6, and IL-10 were higher in the RVVC patients than VVC patients. IL-17A and IL-2 levels were comparable between the two groups. Taken together, our results suggest that the host-immune responses, especially Th1/2 immunity, may play important roles in prognosis of RVVC and VVC.
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http://dx.doi.org/10.3389/fimmu.2022.959740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366074PMC
August 2022

Therapeutic aptamer targeting sclerostin loop3 for promoting bone formation without increasing cardiovascular risk in osteogenesis imperfecta mice.

Theranostics 2022 18;12(13):5645-5674. Epub 2022 Jul 18.

Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases (TMBJ), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China.

Sclerostin inhibition demonstrated bone anabolic potential in osteogenesis imperfecta (OI) mice, whereas humanized therapeutic sclerostin antibody romosozumab for postmenopausal osteoporosis imposed clinically severe cardiac ischemic events. Therefore, it is desirable to develop the next generation sclerostin inhibitors to promote bone formation without increasing cardiovascular risk for OI. Our data showed that sclerostin suppressed inflammatory responses, prevented aortic aneurysm (AA) and atherosclerosis progression in mice. Either loop2&3 deficiency or inhibition attenuated sclerostin's suppressive effects on expression of inflammatory cytokines and chemokines , whilst loop3 deficiency maintained the protective effect of sclerostin on cardiovascular system both and . Moreover, loop3 was critical for sclerostin's antagonistic effect on bone formation in mice. Accordingly, a sclerostin loop3-specific aptamer aptscl56 was identified by our lab. It could recognize both recombinant sclerostin and sclerostin in the serum of OI patients via targeting loop3. PEG40k conjugated aptscl56 (Apc001PE) demonstrated to promote bone formation, increase bone mass and improve bone microarchitecture integrity in mice via targeting loop3, while did not show influence in inflammatory response, AA and atherosclerosis progression in mice with Angiotensin II infusion. Further, Apc001PE had no influence in the protective effect of sclerostin on cardiovascular system in mice, while it inhibited the antagonistic effect of sclerostin on bone formation in mice via targeting loop3. Apc001PE was non-toxic to healthy rodents, even at ultrahigh dose. Apc001PE for OI was granted orphan drug designation by US-FDA in 2019 (DRU-2019-6966). Sclerostin loop3-specific aptamer Apc001PE promoted bone formation without increasing cardiovascular risk in OI mice.
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http://dx.doi.org/10.7150/thno.63177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373813PMC
August 2022

cRGD peptide incorporated with patchouli alcohol loaded silk fibroin nanoparticles for enhanced targeting of inflammatory sites in colitis.

Biomater Adv 2022 Aug 6;140:213069. Epub 2022 Aug 6.

Center for Pharmaceutics Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, China. Electronic address:

The combination therapy of strengthening inflammation regression and mucosal repair may overcome the "therapeutic ceiling" of ulcerative colitis (UC). However, poor targeting is an outstanding challenge in the preparation of drug delivery systems for UC treatment. Here, we developed anti-inflammatory drug (patchouli alcohol, PA)-loaded nanoparticles (NPs) derived from natural silk fibroin (SF) and subjected to surface functionalization with cyclo RGD peptide (cRGD). Self-assembled SF NPs realized sustained drug release. Meanwhile, cRGD functionalization yielded notably targeted drug delivery to inflamed colon, and thereby enhanced the anti-inflammatory and barrier repair capabilities of NPs. Moreover, cRGD-PASFNs regulated innate immune responses and exerted a potent therapeutic efficacy against acute colitis. Surprisingly, the cRGD-PASFNs also modulated the abnormal level of amino acids which are crucial to the integrity of the intestinal barrier. Additionally, oral delivery of this nanomedicine displayed an excellent safety profile in the mouse model. This study confers confidence for the further development of targeted precision therapy for UC and other inflammatory diseases.
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http://dx.doi.org/10.1016/j.bioadv.2022.213069DOI Listing
August 2022

Remission of symptoms is not equal to functional recovery: Psychosocial functioning impairment in major depression.

Front Psychiatry 2022 26;13:915689. Epub 2022 Jul 26.

The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China.

The ultimate goal of depression treatment is to achieve functional recovery. Psychosocial functioning is the main component of functional impairment in depressed patients. The concept of psychosocial functioning has an early origin; however, its concept and connotation are still ambiguous, which is the basic and key problem faced by the relevant research and clinical application. In this study, we start from the paradox of symptoms remission and functional recovery, describe the concept, connotation, and characteristics of psychosocial functioning impairment in depressed patients, and re-emphasize its importance in depression treatment to promote research and clinical applications related to psychosocial functioning impairment in depressed patients to achieve functional recovery.
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http://dx.doi.org/10.3389/fpsyt.2022.915689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360322PMC
July 2022

Synthesis and Discovery of Ligustrazine-Heterocycle Derivatives as Antitumor Agents.

Front Chem 2022 25;10:941367. Epub 2022 Jul 25.

Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Biomedical Material, Tianjin, China.

Ligustrazine (TMP) is a natural pyrazine alkaloid extracted from the roots of Ligusticum Chuanxiong Hort, which has the potential as an antitumor agent. A series of 33 ligustrazine-heterocycle (TMPH) derivatives were designed, synthesized, and investigated via antitumor screening assays, molecular docking analysis, and prediction of drug-like properties. TMP was attached to other heterocyclic derivatives by an 8-12 methylene alkyl chain as a linker to obtain 33 TMPH derivatives. The structures were confirmed by H-NMR, C-NMR, and high-resolution mass spectroscopy spectral (HR-MS) data. The antiproliferative activity against human breast cancer MCF-7, MDA-MB-231, mouse breast cancer 4T1, mouse fibroblast L929, and human umbilical vein endothelial HUVEC cell lines was evaluated by MTT assay. Compound displayed significant inhibitory activity with IC values in the low micromolar range (0.84 ± 0.02 µM against the MDA-MB-231 cell line). The antitumor effects of compound were further evaluated by plate cloning, Hoechst 33 342 staining, and annexin V-FITC/PI staining. The results indicated that compound inhibited the proliferation and apoptosis of breast cancer cells. Furthermore, molecular docking of compound into the active site of the Bcl-2, CASP-3, and PSMB5 target proteins was performed to explore the probable binding mode. The 33 newly synthesized compounds were predicted to have good drug-like properties in a theoretical study. Overall, these results indicated that compound inhibited cell proliferation through PSMB5 and apoptosis through Bcl-2/CASP-3 apoptotic signaling pathways and had good drug-like properties. These results provided more information, and key precursor lead derivatives, in the search for effective bioactive components from Chinese natural medicines.
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http://dx.doi.org/10.3389/fchem.2022.941367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358002PMC
July 2022

Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress.

Int J Mol Sci 2022 Aug 8;23(15). Epub 2022 Aug 8.

State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.

Calcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether plays a role in the response to water deficiency and osmotic stress in potato plants ( L.). Potato cv. Zihuabai was cultivated under natural, moderate, and severe water deficiency conditions; to induce osmotic stress, potato plants were treated with 10% or 20% PEG. -overexpression and -knockdown plants were constructed. were evaluated by qRT-PCR. The subcellular location of the StCDPK28 protein was observed with confocal scanning laser microscopy. Phenotypic changes were indicated by photosynthetic activity, the contents of HO, MDA and proline, and the activities of CAT, SOD and POD. Results showed water deficiency and osmotic stress altered expression patterns. StCDPK28 exhibited a membrane, cytosolic and nuclear localization. Water deficiency and osmotic stress induced upregulation. Photosynthetic activity was enhanced by overexpression, while decreased by knockdown under water deficiency and osmotic stress. overexpression decreased HO and MDA, and increased proline, while knockdown showed reverse results, compared with the wild type, in response to water deficiency and osmotic stress. overexpression increased the activities of CAT, SOD and POD, while -knockdown plants indicated the reverse trend under water deficiency and osmotic stress conditions. Regulation of StCDPK28 expression could be a promising approach to improve the tolerance ability of potato plants in response to drought or high salt media.
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http://dx.doi.org/10.3390/ijms23158795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368905PMC
August 2022

CREB-associated glycosylation and function in human disease.

Adv Clin Exp Med 2022 Aug 11. Epub 2022 Aug 11.

Division of Oncology, The Affiliated Ganzhou Hospital of Nanchang University, China.

The shortcomings of mRNA sequencing in explaining biological functions have resulted in proteomics gradually becoming a hotspot for research. However, the function of proteins becomes complicated as a result of post-translational modifications (PTMs) such as phosphorylation, glycosylation, acetylation, etc. Post-translational modifications do not change the physicochemical properties such as charge and solubility of the proteins, but they can have significant consequences on disease initiation in living organisms. The cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) is an important transcription regulator in eukaryotic cells. It is involved in the development of neurodegenerative diseases, diabetic complications, tumorigenesis, and neurogenesis. Previously, researchers have paid much more attention to the phosphorylation modification of CREB. However, it seems that the functional regulation-mediated glycosylation modification of CREB was just beginning to be understood. In this review, the current studies and most updated insights on how the glycosylation modification of CREB affects targeted gene expression and disease development will be comprehensively discussed. We hope to further evaluate the role of CREB glycosylation on the regulation of gene function.
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http://dx.doi.org/10.17219/acem/151026DOI Listing
August 2022

Surgical and oncological outcomes of distal gastrectomy compared to total gastrectomy for middle-third gastric cancer: A systematic review and meta-analysis.

Oncol Lett 2022 Sep 4;24(3):291. Epub 2022 Jul 4.

Department of General Surgery, Center for Minimally Invasive Gastrointestinal Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.

Distal gastrectomy (DG) and total gastrectomy (TG) are the most common types of radical surgery for patients with middle-third gastric cancer (MTGC). However, the indications and benefits of the two procedures still remain controversial. The present meta-analysis aimed to compare the surgical and oncological outcomes of DG and TG in the treatment of MTGC. A rigorous literature review was performed in the databases of PubMed, Embase, Web of Science, China National Knowledge Infrastructure and Chinese BioMedical Literature to retrieve studies published up to February 2022. The Newcastle-Ottawa Scale was used to assess the quality of included studies and a meta-analysis was performed using RevMan 5.3 software. A total of 12 retrospective studies performing comparisons of DG and TG were included in the present meta-analysis. For patients who underwent DG, a lower rate of overall post-operative complications, anastomosis leakage and intro-abdominal infection was determined. No significant difference was observed between DG and TG in the 5-year overall survival when the proximal resection margin ranged from 3 to 5 cm. Although DG was associated with a higher 5-year overall survival rate when compared to TG, there was no significant difference in the stratified analyses by TNM stage. In conclusion, the prognosis of MTGC did not depend on the extent of gastrectomy. With lower complications and acceptable oncological outcomes, DG was a safe and feasible surgical procedure for MTGC when a negative proximal margin was confirmed.
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http://dx.doi.org/10.3892/ol.2022.13411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353235PMC
September 2022

Composite hydrolytic acidification - aerobic MBBR process for treating traditional Chinese medicine wastewater.

Biodegradation 2022 Aug 10. Epub 2022 Aug 10.

School of Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin, 150090, China.

Traditional Chinese medicine (TCM) wastewater is characterized by high organic content, unstable water quality and quantity and low biodegradability. In this paper, the hydrolytic acidification reactor-aerobic moving bed biofilm (MBBR) process was used to degrade TCM wastewater. Besides, a small pilot study was conducted. The appropriate operating parameters: hydraulic retention time (HRT) of the hydrolytic reactor was 16 h, HRT of MBBR was 30 h, dissolved oxygen of MBBR was 6 mg/L, sludge return ratio of MBBR was 100%. The hydrolytic reactor was started for 25 days. MBBR was run in series with the hydrolytic reactor after 24 days of separate operation. The start-up of the composite reactor was completed after another 26 days. The average removal efficiencies of chemical oxygen demand and ammonia nitrogen were 92% and 70%. The hydrolytic reactor was effective in decomposing macromolecules and MBBR had a strong ability to degrade pollutants through the excitation-emission-matrix spectra. The evolution pattern of the dominant bacterial genera and the surface morphology of sludge were studied by scanning electron microscopy and high-throughput sequencing analysis. It could be seen that the surface morphology of the biological filler was suitable for the growth and reproduction of microorganisms.
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http://dx.doi.org/10.1007/s10532-022-09995-wDOI Listing
August 2022

HDAC3 Inhibitor RGFP966 Ameliorated Neuroinflammation in the Cuprizone-Induced Demyelinating Mouse Model and LPS-Stimulated BV2 Cells by Downregulating the P2X7R/STAT3/NF-κB65/NLRP3 Activation.

ACS Chem Neurosci 2022 Aug 10. Epub 2022 Aug 10.

Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, Shandong 252000, China.

Suppression of excessive microglial overactivation can prevent the progression of multiple sclerosis (MS). Histone deacetylases 3 inhibitor (HDAC3i) has been demonstrated to exert anti-inflammatory effects by suppressing microglia (M1-liked) activation. Here, we demonstrate that the RGFP966 (a selective inhibitor of HDAC3) protects white matter after cuprizone-induced demyelination, as shown by reductions in neurological behavioral deficits and increases in myelin basic protein. Moreover, in this study, we found that RGFP966 caused a significant reduction in the levels of inflammatory cytokines, including IL-1β, TNF-α, as well as iNOS, and inhibited microglial (M1-liked) activation in the experimental cuprizone model and LPS-stimulated BV2 cells. Meanwhile, RGFP966 alleviated apoptosis of LPS-induced BV2 cells in vitro. Furthermore, RGFP966 suppressed the expression of P2X7R, NLRP3, ASC, IL-18, IL-1β, and caspase-1, inhibited the ratio of phosphorylated-STAT3/STAT3 and phosphorylated NF-κB p65/NF-κB p65, as well as increased acetylated NF-κB p65 in vitro and in vivo. Furthermore, we confirmed that brilliant blue G (antagonists of P2X7R) suppressed the expression of microglial NLRP3, IL-18, IL-1β, caspase-1, NF-κB p65 (including phosphorylated NF-κB p65), and STAT3 (including phosphorylated STAT3) in vitro. These findings demonstrated that RFFP966 alleviated the inflammatory response and exerted a neuroprotective effect possibly by modulating P2X7R/STAT3/NF-κB65/NLRP3 signaling pathways. Thus, HDAD3 might be considered a promising intervention target for neurodegenerative diseases, such as MS.
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http://dx.doi.org/10.1021/acschemneuro.1c00826DOI Listing
August 2022

A patched1 gene homologue participates in female differentiation of Cynoglossus semilaevis.

Gene Expr Patterns 2022 Aug 6;45:119265. Epub 2022 Aug 6.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science (CAFS), Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

Patched (Ptch) is a receptor in the hedgehog signaling pathway, essential for animal development. Our previous study showed that ptch1 gene participates in the maintenance of the male germline and spermatogenesis in Cynoglossus semilaevis (csptch1). In this study, we identified a patched1 gene homolog (csptch1 x1). The csptch1 x1 gene is 5761 bp long, with a 4638 bp coding sequence that encodes 1545 amino acids. The Csptch1 x1 protein has 12 transmembrane regions and sterol-sensing domains and is highly homologous to the csptch1 (91 amino acids difference). Expression pattern analysis showed that csptch1 x1 is expressed in eight different tissues of adult tongue sole, and the expression is significantly higher in tissues of female than that in male tissues. The expression pattern in developmental stages was also analyzed. csptch1 x1 could be detected at the 1-cell stage and was highly expressed at the blastocyst, somite, and blastopore closing stages, implying that it participates in cell differentiation. In ovarian development, the expression of csptch1 x1 was initiated at 20 days after hatching (dah) and was significantly high at 35-50 and 95-150 dah. In situ hybridization showed that csptch1 x1 was predominantly expressed in primordial germ cells, oocytes, and follicular cells, but the expression of the gene was lower in the testis. These results suggest that csptch1 x1 may be mainly involved in female differentiation and ovarian development, different from the role of csptch1 in spermatogenesis.
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http://dx.doi.org/10.1016/j.gep.2022.119265DOI Listing
August 2022

Mn oxides enhanced pyrene removal with both rhizosphere and non-rhizosphere microorganisms in subsurface flow constructed wetlands.

Chemosphere 2022 Aug 6;307(Pt 2):135821. Epub 2022 Aug 6.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, 250100, China.

The polycyclic aromatic hydrocarbons (PAHs) are substantial wastewater pollutants emitted mostly by petroleum refineries and petrochemical industries, and their environmental fate has been of increasing concern among the public. Consequently, subsurface flow constructed wetlands (SFCWs) filled with Mn oxides (W-CW) or without Mn oxides (K-CW) were established to investigate the performance and mechanisms of pyrene (PYR) removal. The average removal rates of PYR in W-CW and K-CW were 96.00% and 92.33%, respectively. The PYR removal via other pathways (microbial degradation, photolysis, volatilisation, etc.) occupied a sizeable proportion, while the total PYR content in K-CW plant roots was significantly higher than that of W-CW. The microorganisms on the root surface and rhizosphere played an important role in PYR degradation in W-CW and K-CW and were higher in W-CW than that in K-CW in all matrix zones. The microorganisms between the 10-16 cm zone from the bottom of W-CW filled with Mn oxides (W-16) were positively correlated with PYR-degrading microorganisms, aerobic bacteria and facultative anaerobes, whereas K-16 without birnessite-coated sand was negatively correlated with these microorganisms.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135821DOI Listing
August 2022

The Value of Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) in the Differentiation of Pseudoprogression and Recurrence of Intracranial Gliomas.

Contrast Media Mol Imaging 2022 22;2022:5680522. Epub 2022 Jul 22.

College of Medical Imaging, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.

Objective: The objective of this study was to determine the value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in assessing postoperative changes in intracranial gliomas.

Method: A total of fifty-one patients who had new enhanced lesions after surgical resection followed by standard radiotherapy and chemotherapy were collected retrospectively from October 2014 to June 2021. The patients were divided into a pseudoprogression group (15 cases) and a recurrence group (36 cases) according to the pathological results of the second operation or a follow-up of more than six months. The follow-up data of all patients were complete, and DCE-MRI was performed. The images were processed to obtain the quantitative parameters , Ve, and Kep and the semiquantitative parameter iAUC, which were analysed with relevant statistical software.

Results: First, the difference in and iAUC values between the two groups was statistically significant (  ), and the difference in Ve and Kep values was not statistically significant (  ). Second, by comparing the area under the curve, threshold, sensitivity and specificity of , and iAUC, it was found that the iAUC threshold value was slightly higher than that of , and the specificity of was equal to that of iAUC, while the area under the curve and sensitivity of were higher than those of iAUC. Third, and iAUC had high accuracy in diagnosing recurrence and pseudoprogression, and had higher accuracy than iAUC.

Conclusion: In this study, DCE-MRI has a certain diagnostic value in the early differentiation of recurrence and pseudoprogression, offering a new method for the diagnosis and assessment of gliomas after surgery.
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http://dx.doi.org/10.1155/2022/5680522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337951PMC
August 2022

A novel approach for forensic identification of automotive paints using optical coherence tomography and multivariate statistical methods.

J Forensic Sci 2022 Aug 1. Epub 2022 Aug 1.

Institute of Forensic Science, Ministry of Public Security, Beijing, China.

Automotive paint is one of the most important evidence in solving vehicle-related criminal cases. It contains the critical information about the suspected vehicle, providing essential clues for the investigation. In this study, a novel approach based on optical coherence tomography combined with multivariate statistical methods was proposed to facilitate rapid, accurate and nondestructive identification of different brands of automotive paints. 164 automotive paint samples from 8 different manufacturers were analyzed by a spectral-domain optical coherence tomography system (SD-OCT). Two-dimensional cross-sectional OCT images and three-dimensional OCT reconstruction of vehicle paints of different paints were obtained to show the internal structural differences. Visual discrimination of A-scan data after registration and averaging processing was first used to distinguish different samples. An scanning electron microscope was utilized to obtain the cross-sectional image of the sample to evaluate the effectiveness of OCT technique. Then the original A-scan data, first derivative data and second derivative data of 136 paints with four layers from 7 different manufacturers were collected. Multivariate statistical methods, including principal component analysis (PCA), multi-layer perceptron (MLP), k-nearest neighbor (KNN) algorithm and Bayes discriminant analysis (BDA), were used to analyze different datasets. The results show the hybrid PCA and BDA model based on the first derivative OCT data achieved the best result of 100% accuracy on the testing dataset for identifying automotive paints. It is demonstrated that the OCT technique combined with multivariate statistics could be a promising method for identifying the automotive paints rapidly and accurately.
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http://dx.doi.org/10.1111/1556-4029.15114DOI Listing
August 2022

The Value of Laparoscopic Simultaneous Colorectal and Hepatic Resection for Synchronous Colorectal Cancer Liver Metastasis: A Propensity Score Matching Study.

Front Oncol 2022 12;12:916455. Epub 2022 Jul 12.

Department of Hepatic Surgery, Shanghai Cancer Center, Fudan University, Shanghai, China.

Purpose: The aim of this study is to investigate the value of total laparoscopic simultaneous colorectal and hepatic resection in patients with synchronous colorectal cancer liver metastases (sCRLMs).

Methods: sCRLM patients who underwent simultaneous resection from December 2014 to December 2018 in Shanghai Cancer Center, Fudan University were recruited and analyzed retrospectively. The patients were divided into laparoscopic, open, and hybrid surgery groups. The intraoperative information, postoperative short-term outcome, and long-term survival were compared among the three groups. Propensity score matching (PSM) was performed to balance baselines.

Results: A total of 281 patients were recruited. After PSM, 34 patients were selected from both the laparoscopic and the open surgery group. Forty-seven patients were also selected from both the laparoscopic and the hybrid surgery group. The clinicopathologic baselines between the laparoscopic surgery group and the other two groups were well matched. All the operation-related indicators between laparoscopic surgery and hybrid surgery were similar. However, compared with open surgery, laparoscopic surgery showed significantly longer operation time (229.09 ± 10.94 min vs. 192.24 ± 9.49 min, = 0.013) and less intraoperative blood loss [100.00 (50.00-300.00) ml vs. 200.00 (150.00-400.00) ml, = 0.021]. For postoperative morbidity, there was no significant difference between the laparoscopic surgery group and the hybrid or the open surgery group (23.40% vs. 31.91% and 17.65% vs. 26.47%, = 0.356 and = 0.380). Long-term survival analysis showed that there were no significant differences in all 1-, 3-, and 5-year overall survival, liver recurrence-free survival (RFS), and whole RFS between laparoscopic surgery and hybrid surgery ( = 0.334, = 0.286, and = 0.558) or open surgery ( = 0.230, = 0.348, and = 0.450).

Conclusions: Laparoscopic simultaneous resection for sCRLM shows slight advantages in surgical safety and short-term outcome, and does not compromise long-term survival.
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http://dx.doi.org/10.3389/fonc.2022.916455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315101PMC
July 2022

Bcl-2 and Noxa are potential prognostic indicators for patients with gastroenteropancreatic neuroendocrine neoplasms.

Endocrine 2022 Jul 27. Epub 2022 Jul 27.

Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China.

Purpose: Bcl-2 family proteins are of great significance in the pathogenesis and development of tumors. In this study, the correlations between the expression of Bcl-2 family proteins and clinicopathological features and prognosis of neuroendocrine neoplasms (NENs) were further investigated.

Methods: 105 Patients diagnosed with gastroenteropancreatic NENs (GEP-NENs) with the paraffin specimen of the tumor available were retrospectively included. Immunohistochemistry (IHC) was performed to detect the expression of Bcl-2 family proteins in paraffin-embedded samples. Student's t-test and Chi-square test were applied to compare the difference of quantitative and categorical variables, respectively. Survival analysis was conducted according to Kaplan-Meier method. Univariate and multivariate cox regression analysis were used to identify the independent prognostic factors.

Results: The IHC score of Bcl-2 was significantly higher in neuroendocrine carcinoma (NEC) patients (65.6%), while a higher IHC score of Noxa was more common in neuroendocrine tumor (NET) patients (49.3%). Survival analysis indicated that patients with higher Bcl-2 expression and lower Noxa expression had worse 5-year survival (39.3% vs. 75.6%, p < 0.001; 40.6% vs. 84.9%, p < 0.001). Multivariate cox analysis indicated that high Bcl-2 expression was an independent factor associated with inferior DFS (hazard ratio [HR]: 2.092; 95% confidence interval [CI]: 1.106-3.955; p = 0.023) and OS (HR: 2.784; 95% CI: 1.326-5.846; p = 0.007), while higher Noxa expression was associated with superior DFS (HR:0.398; 95% CI: 0.175-0.907; p = 0.028) and OS (HR: 0.274; 95% CI: 0.110-0.686; p = 0.006).

Conclusions: Higher expression of Bcl-2 and lower expression of Noxa were associated with unfavorable prognosis of GEP-NENs patients.
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http://dx.doi.org/10.1007/s12020-022-03114-8DOI Listing
July 2022

Temperature Fluctuations Compensation with Multi-Frequency Synchronous Manipulation for a NV Magnetometer in Fiber-Optic Scheme.

Sensors (Basel) 2022 Jul 12;22(14). Epub 2022 Jul 12.

The School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China.

Nitrogen-vacancy (NV) centers in diamonds play a large role in advanced quantum sensing with solid-state spins for potential miniaturized and portable application scenarios. With the temperature sensitivity of NV centers, the temperature fluctuations caused by the unknown environment and the system itself will mix with the magnetic field measurement. In this research, the temperature-sensitive characteristics of different diamonds, alongside the temperature noise generated by a measurement system, were tested and analyzed with a homemade NV magnetometer in a fiber-optic scheme. In this work, a multi-frequency synchronous manipulation method for resonating with the NV centers in all axial directions was proposed to compensate for the temperature fluctuations in a fibered NV magnetic field sensing scheme. The symmetrical features of the resonance lines of the NV centers, the common-mode fluctuations including temperature fluctuations, underwent effective compensation and elimination. The fluorescence change was reduced to 1.0% by multi-frequency synchronous manipulation from 5.5% of the single-frequency manipulation within a ±2 °C temperature range. Additionally, the multi-frequency synchronous manipulation improved the fluorescence contrast and the magnetic field measurement SNR through an omnidirectional manipulation scheme. It was very important to compensate for the temperature fluctuations, caused by both internal and external factors, to make use of the NV magnetometer in fiber-optic schemes' practicality. This work will promote the rapid development and widespread applications of quantum sensing based on various systems and principles.
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http://dx.doi.org/10.3390/s22145218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320826PMC
July 2022

, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean ( Sieb. and Zucc).

Int J Mol Sci 2022 Jul 8;23(14). Epub 2022 Jul 8.

Key Laboratory of Soybean Biology of Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, College of Science, Northeast Agricultural University, Harbin 150030, China.

Soybeans are a major crop that produce the best vegetable oil and protein for use in food and beverage products worldwide. However, one of the most well-known viral infections affecting soybeans is the Soybean Mosaic Virus (SMV), a member of the family. A crucial method for preventing SMV damage is the breeding of resistant soybean cultivars. Adult resistance and resistance of seedcoat mottling are two types of resistance to SMV. Most studies have focused on adult-plant resistance but not on the resistance to seedcoat mottling. In this study, chromosome segment-substituted lines derived from a cross between Suinong14 (cultivated soybean) and ZYD00006 (wild soybean) were used to identify the chromosome region and candidate genes underlying soybean resistance to seed coat mottling. Herein, two quantitative trait loci (QTLs) were found on chromosome 17, and eighteen genes were found in the QTL region. RNA-seq was used to evaluate the differentially expressed genes (DEGs) among the eighteen genes located in the QTLs. According to the obtained data, variations were observed in the expression of five genes following SMV infection. Furthermore, was subjected to an -mediated transient expression assay to investigate the role of the five candidate genes in SMV resistance. It has also been revealed that encoding a RICE SALT SENSITIVE 3-like protein (RSS3L) can inhibit the multiplication of SMV in . Moreover, two nonsynonymous single-nucleotide polymorphisms (SNPs) were found in the coding sequence of derived from the wild soybean ZYD00006 (), and the two amino acid mutants may be associated with SMV resistance. Hence, it has been suggested that confers seedcoat mottling resistance, shedding light on the mechanism of soybean resistance to SMV.
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http://dx.doi.org/10.3390/ijms23147577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318458PMC
July 2022

Development and Evaluation of an Anti-Biotin Interference Method in Biotin-Streptavidin Immunoassays.

Diagnostics (Basel) 2022 Jul 16;12(7). Epub 2022 Jul 16.

Department of Clinical Laboratory, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

The strong non-covalent interaction between biotin and streptavidin places streptavidin-based assays, used by many laboratories, at an increased risk of interference by biotin. At present, a few manufacturers have developed fully automated anti-biotin interference methods, although compared with many detection platforms, these remain insufficient. Additionally, there is a need for more methods that can achieve fully automated anti-biotin interference. We sought to develop and evaluate a new biotin interference-resisting method based on a biotin-streptavidin chemiluminescence immunoassay. Streptavidin-coated magnetic microparticles (M) of different concentrations were prepared and tested for their biotin-resistance capabilities in an automated setting (Cobas e 601). The precision, accuracy, and detection capability were also assessed. Higher concentrations of M were found to have a stronger ability to resist biotin interference. A 2.16 mg/mL concentration of M was able to resist 500 ng/mL of biotin in samples while simultaneously having a relatively weak shielding effect on the optical signals. Moreover, the total precision and accuracy of this method, designated as M3, met acceptable standards. M3 has an improved ability to resist biotin interference, can achieve full automation, and its detection performance can meet the general laboratory quality requirements.
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http://dx.doi.org/10.3390/diagnostics12071729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324022PMC
July 2022

[email protected] Composite Film as a Fluorescence Sensor for Detection of Chilled Pork Freshness.

Biosensors (Basel) 2022 Jul 20;12(7). Epub 2022 Jul 20.

Research Center of Information Technology, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China.

This study presents a novel composite thin film based on rhodamine B encapsulated into MOF-5 (Metal Organic Frameworks) as a fluorescence sensor for the real-time detection of the freshness of chilled pork. The composite film can adsorb and respond to the volatile amines produced by the quality deterioration of pork during storage at 4 °C, with the fluorescence intensity of RhB decreasing over time. The quantitative model used for predicting the freshness indicator (total volatile base nitrogen) of pork was built using the fluorescence spectra (excited at 340 nm) of the [email protected] composite film combined with the partial least squares (PLS) algorithm, providing R and R values of 0.908 and 0.821 and RMSEC (root mean square error of calibration) and RMSEP (root mean square error of prediction) values of 3.435 mg/100 g and 3.647 mg/100 g, respectively. The qualitative model established by the partial least squares discriminant analysis (PLS-DA) algorithm was able to accurately classify pork samples as fresh, acceptable or spoiled, and the accuracy was 86.67%.
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http://dx.doi.org/10.3390/bios12070544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313163PMC
July 2022

Rational development of a combined mRNA vaccine against COVID-19 and influenza.

NPJ Vaccines 2022 Jul 26;7(1):84. Epub 2022 Jul 26.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.

As the world continues to experience the COVID-19 pandemic, seasonal influenza remain a cause of severe morbidity and mortality globally. Worse yet, coinfection with SARS-CoV-2 and influenza A virus (IAV) leads to more severe clinical outcomes. The development of a combined vaccine against both COVID-19 and influenza is thus of high priority. Based on our established lipid nanoparticle (LNP)-encapsulated mRNA vaccine platform, we developed and characterized a novel mRNA vaccine encoding the HA antigen of influenza A (H1N1) virus, termed ARIAV. Then, ARIAV was combined with our COVID-19 mRNA vaccine ARCoV, which encodes the receptor-binding domain (RBD) of the SARS-CoV-2 S protein, to formulate the final combined vaccine, AR-CoV/IAV. Further characterization demonstrated that immunization with two doses of AR-CoV/IAV elicited robust protective antibodies as well as antigen-specific cellular immune responses against SARS-CoV-2 and IAV. More importantly, AR-CoV/IAV immunization protected mice from coinfection with IAV and the SARS-CoV-2 Alpha and Delta variants. Our results highlight the potential of the LNP-mRNA vaccine platform in preventing COVID-19 and influenza, as well as other respiratory diseases.
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http://dx.doi.org/10.1038/s41541-022-00478-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315335PMC
July 2022

Aromatic diglycosides from and a multi-step conformer filtering procedure for TDDFT calculation of flexible glycoside.

J Asian Nat Prod Res 2022 Jul 26:1-11. Epub 2022 Jul 26.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Three previously undescribed aromatic diglycosides (, , and ) and six known analogs (, , , and ) were isolated from the roots and rhizomes of Gagnep. Their structures were elucidated by detailed spectroscopic analysis. The absolute configuration of compound was determined by comparing the experimental and TDDFT calculated ECD spectra of and aglycone . Furthermore, a multistep conformer filtering procedure for TDDFT calculation of flexible glycoside was proposed, which afforded high accuracy with acceptable computing cost in determining the absolute configuration of glycosides using quantum calculated ECD.
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http://dx.doi.org/10.1080/10286020.2022.2100359DOI Listing
July 2022

[Effect of mild moxibustion on SIRT1/NF-κB signaling in atherosclerotic rabbits].

Zhen Ci Yan Jiu 2022 Jul;47(7):598-604

College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha 410208, China; College of Acupuncture-moxibustion and Massage, Guizhou University of Chinese Medicine, Guiyang 550025.

Objective: To observe the effect of mild moxibustion on blood lipid, histopathological structure of the aortic arch, thoracic aortic silent information regulator 1 (SIRT1)/nuclear factor κB (NF-κB) signaling pathway in atherosclerosis (AS) rabbits, so as to explore its underlying mechanisms in improving AS.

Methods: Sixty male rabbits were randomly divided into control group (=12), model group(=11), mild moxibustion group (=11), mild moxibustion + blocker (blocker) group (=12). The AS model was established by feeding the rabbits with high-fat forage for 8 weeks, followed by immune response damage. Mild moxibustion was applied to "Danzhong"(CV17), "Shenque"(CV8) and "Neiguan" (PC6, bilateral) and "Xuehai" (SP10, bilateral) for 30 min, once daily, 3 times a week for 4 weeks. Rabbits of the blocker group received intraperitoneal injection of EX527 (a selective inhibitor of SIRT1, 5 mg·kg·d) 30 min before moxibustion. Rabbits of the control and model groups were only grabbed and fixed without intervention. After the intervention, the contents of serum triglyceride (TG) and total cholesterol (TC) were determined by enzymatic method, and those of serum low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were determined by colorimetric method. The Sudan Ⅳ staining was employed to observe the histopathological structure of the aortic arch, and Western blot and fluorescence quantitative real time-PCR were used to detect the expressions of SIRT1 and NF-κB proteins and mRNAs in the thoracic aorta, respectively.

Results: Compared with the control group, the contents of serum TG, TC and LDL-C and the expression levels of NF-κB protein and mRNA were significantly increased (<0.01, <0.05), whereas the content of HDL-C and the expression of SIRT1 mRNA markedly decreased in the model group (<0.01). After mild moxibustion, the contents of serum TG, TC, and LDL-C and the expression of NF-κB protein and mRNA were significantly down-regulated (<0.01, <0.05), while the content of HDL-C and the expression levels of SIRT1 protein and mRNA significantly up-regulated in the mild moxibustion group (<0.05, <0.01). There were no significant differences between the blocker and model groups in all the indexes (>0.05). Compared with the mild moxibustion group, the serum TG, TC, and LDL-C contents and NF-κB protein expression were significantly increased (<0.01, <0.05), and HDL-C content and the expression of SIRT1 protein and mRNA significantly decreased (<0.05, <0.01) in the blocker group. Sudan Ⅳ staining showed vague structure of the aortic arch with obvious lipid infiltration in the model group, which was relatively milder in the mild moxibustion.

Conclusion: Mild-moxibustion can reduce blood lipid levels and endothelial damage in atherosclerotic rabbits, which may be related to its function in regulating SIRT1/NF-κB signaling pathway.
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http://dx.doi.org/10.13702/j.1000-0607.20210637DOI Listing
July 2022
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