Publications by authors named "Ning Yang"

971 Publications

Irisin as a Potential Biomarker Associated with Myocardial Injuries in Patients with Severe Hypothyroidism.

Int J Endocrinol 2021 18;2021:3116068. Epub 2021 Nov 18.

Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.

Objective: Irisin, a novel myokine, has recently been considered to produce a cardioprotective effect. Potential biomarkers for myocardial injuries in patients with severe hypothyroidism have yet to be identified. We aimed to investigate whether serum irisin may serve as a promising biomarker for early detecting the myocardial injuries in patients with severe hypothyroidism.

Methods: This cross-sectional study comprised 25 newly diagnosed drug-naive patients with severe primary hypothyroidism and 17 age- and sex-matched healthy controls. Circulating irisin levels and cardiac magnetic resonance (CMR) were evaluated in each participant. Left ventricular (LV) myocardial injuries were detected by CMR-based T1 mapping technique using a modified look-locker inversion recovery (MOLLI) sequence, which is quantified as native T1 values.

Results: Compared with healthy controls, the severe hypothyroidism group had significantly lower levels of serum irisin, especially those with pericardial effusion ( < 0.05). The severe hypothyroidism subjects exhibited lower peak filling rates (PFRs) and higher native myocardial T1 values than controls ( < 0.05). Additionally, the ROC analysis displayed that the sensitivity and specificity of serum irisin for diagnosing pericardial effusion in patients with severe hypothyroidism were 73.3% and 100.0%, respectively. The AUC was 0.920 (0.861-1.000) ( < 0.001). The cutoff value was 36.94 ng/mL. Moreover, the results in subgroup analysis revealed that the native T1 values of the low-irisin group were significantly higher than that of the high-irisin group ( < 0.05). According to multivariate linear regression analysis, serum irisin concentrations were negatively and independently correlated with native myocardial T1 values after adjustment for age, sex, and other conventional confounding factors ( = -1.473, < 0.05).

Conclusions: Irisin may be a potential biomarker for predicting myocardial injuries in patients with severe hypothyroidism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/3116068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616683PMC
November 2021

Identifying the Predictive Role of Oxidative Stress Genes in the Prognosis of Glioma Patients.

Med Sci Monit 2021 Nov 27;27:e934161. Epub 2021 Nov 27.

Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, Shandong, China (mainland).

BACKGROUND Gliomas are primary aggressive brain tumors with poor prognoses. Oxidative stress plays a crucial role in the tumorigenesis and drug resistance of gliomas. The aim of the present study was to use integrated bioinformatics analyses to evaluate the prognostic value of oxidative stress-related genes (OSRGs) in glioma. MATERIAL AND METHODS Disease- and prognosis-associated OSRGs were identified using microarray and clinical data from the Chinese Glioma Genome Atlas database. Functional enrichment, gene-gene interaction, protein-protein interaction, and survival analyses were performed in screened OSRGs. The protein expression was validated by the Human Protein Atlas database. A risk score model was constructed and verified through Cox regression, receiver operating characteristic curve, principal component, and stratified analyses. The Cancer Genome Atlas (TCGA) database was used for external validation. A nomogram was constructed to facilitate the clinical application. RESULTS Twenty-one disease-associated and 14 prognosis-associated OSRGs were identified. Enrichment analyses indicated that these signature OSRGs were involved in tumorigenesis and drug resistance of glioma. The risk score model demonstrated a significant difference in overall survival between the high- and low-risk groups. The area under the curve and hazard ratio (1.296) revealed the independent prognostic value of the model. The model exhibited good predictive efficacy in the TCGA cohort. A clinical nomogram was constructed to calculate survival rates in glioma patients at 1, 3, and 5 years. CONCLUSIONS Our comprehensive study indicated that OSRGs were valuable for prognosis prediction in glioma, which provides a novel insight into the relationship between oxidative stress and glioma and a potential therapeutic strategy for glioma patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.934161DOI Listing
November 2021

Recent Development of Multifunctional Sensors Based on Low-Dimensional Materials.

Sensors (Basel) 2021 Nov 20;21(22). Epub 2021 Nov 20.

Information Science Academy of China Electronics Technology Group Corporation, Beijing 100086, China.

With the demand for accurately recognizing human actions and environmental situations, multifunctional sensors are essential elements for smart applications in various emerging technologies, such as smart robots, human-machine interface, and wearable electronics. Low-dimensional materials provide fertile soil for multifunction-integrated devices. This review focuses on the multifunctional sensors for mechanical stimulus and environmental information, such as strain, pressure, light, temperature, and gas, which are fabricated from low-dimensional materials. The material characteristics, device architecture, transmission mechanisms, and sensing functions are comprehensively and systematically introduced. Besides multiple sensing functions, the integrated potential ability of supplying energy and expressing and storing information are also demonstrated. Some new process technologies and emerging research areas are highlighted. It is presented that optimization of device structures, appropriate material selection for synergy effect, and application of piezotronics and piezo-phototronics are effective approaches for constructing and improving the performance of multifunctional sensors. Finally, the current challenges and direction of future development are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21227727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618950PMC
November 2021

Durability Optimization of Fiber Grating Hydrogen Sensor Based on Residual Stress.

Sensors (Basel) 2021 Nov 18;21(22). Epub 2021 Nov 18.

College of Civil Engineering, Southeast University, Nanjing 211189, China.

In this paper, in order to improve the durability of optical fiber grating hydrogen sensors, an optical fiber grating hydrogen sensor with high precision, stability, and durability is prepared. Based on the simplified two-dimensional model and finite element analysis, the effects of film thickness, coating speed, and coating times on the residual Mises equivalent stress between the sensor film and substrate were studied, and the optimum coating parameters were determined. The finite element analysis results show that the residual equivalent stress between the film and the substrate increases with the increase in the film thickness between 50 and 150 nm. The range of 200-250 nm is relatively stable, and the value is small. The coating speed has almost no effect on the residual equivalent stress. When the thickness of the film is 200 nm, the residual equivalent stress decreases with the increase in coating times, and the equivalent force is the lowest when the film is coated three times. The best coating parameters are the thickness of 200 nm, the speed of 62.5 μm/s, and the times of coating three times. The results of finite element analysis are verified by the hydrogen sensitivity test and durability test.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21227657DOI Listing
November 2021

Ladder-Type Fused Benzodithiophene Extended along the Short-Axis Direction as a New Donor Building Block for Efficient Organic Solar Cells.

ACS Appl Mater Interfaces 2021 Nov 23. Epub 2021 Nov 23.

Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea.

Ladder-type fused aromatic systems are important core structures of small molecule acceptors for organic solar cells (OSCs). In this study, a new ladder-type donor building block, based on the benzo[1,2-:4,5-']dithiophene (BDT) unit where the 3,7 positions of the BDT thiophene rings and the 3' position of the thiophene rings of the vertical BDT were fused to construct a seven-ring core structure named f-DTBDT, was investigated. In the f-DTBDT structure, the fusion of the BDT core and the thiophene rings at the 4,8 positions of BDT constrains all of the aromatic rings in a coplanar structure. The newly designed f-DTBDT was successfully employed as a core donor building block and conjugated with three electron-withdrawing acceptors (2-(3-oxo-2,3-dihydro-1-inden-1-ylidene) malononitrile (2HIC), 2-(5,6-difluoro-3-oxo-2,3-dihydro-1-inden-1-ylidene)malononitrile (2FIC), and 2-(5,6-dichloro-3-oxo-2,3-dihydro-1-inden-1-ylidene)malononitrile (2ClIC)) as acceptor-donor-acceptor (A-D-A)-type acceptor materials for OSCs. Characterization results showed that the three synthesized A-D-A acceptors of DTBDT-IC, DTBDT-4F, and DTBDT-4Cl have high absorption behavior in the vis-NIR region as result of an intramolecular charge transfer interaction engendered by f-DTBDT and the ending group. The absorption regions of the acceptors were complementary with that of polymer PM6. Also, the frontier orbital energy levels of the new acceptors and wide-band-gap PM6 are well matched. Bulk heterojunction OSCs were fabricated using PM6 and the acceptors, and the highest power conversion efficiency (PCE) of 10.15% was obtained when using PM6:DTBDT-4F as the active layer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c16401DOI Listing
November 2021

The Prevalence and Control of Type 2 Diabetes Mellitus in Residents of a Rural Town, Shandong Province, China.

Diabetes Metab Syndr Obes 2021 11;14:4505-4512. Epub 2021 Nov 11.

Department of Hypertension, TEDA International Cardiovascular Hospital, Tianjin, People's Republic of China.

Objective: To understand the prevalence and control of type 2 diabetes mellitus (T2DM) in a rural area, a cross-sectional study was carried out.

Methods: Blood glucose was determined in residents over 60 years during the National Basic Public Health Service project carrying on in a rural town of Shandong province. Combined with detail information of diabetic history, the prevalence rate of this disease was calculated with a cutoff of more than 7.0 mmol/L of fasting plasma glucose (FPG). Furthermore, according to FPG levels, the control rate of diabetes was evaluated. Basic information and clinical laboratory tests were analyzed with proper methods for univariate analysis. Multivariable logistic analysis was used to explore multiple variables associated with the control effect of T2DM.

Results: The prevalence rate of T2DM was 13.5% (95% CI 12.4%~14.7%) without age difference in residents over 60-year old ( = 1.078, = 0.898). And the rate was higher in the female (16.1%, 95% CI 14.4%~17.8%) than that in the male (11.3%, 95% CI 9.7%~12.9%). The control rate of diabetes was 44.5% (95% CI 39.4-49.6%, cutoff <6.1 mmol/L for FPG) and 59.6% (95% CI 54.5-64.6%, cutoff <7.0 mmol/L for FPG), respectively. Most T2DM residents had balance foods, and a high proportion of drugs or insulin were used; however, the proportion of keeping exercise and modifying behaviors was low. Nearly 80% of the diabetic individuals used at least one drug or insulin. However, the more drugs or insulin used, the higher the FPG level was.

Conclusion: A relative high level of T2DM prevailed in the rural area under fast economy-transforming. T2DM was controlled poor and measures should be further optimized to achieve ideal effect, especially for the use of anti-diabetic drugs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DMSO.S338728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593350PMC
November 2021

The Role of the Immune Microenvironment in Bone Regeneration.

Authors:
Ning Yang Yao Liu

Int J Med Sci 2021 21;18(16):3697-3707. Epub 2021 Sep 21.

Department of Pediatric Dentistry, School and Hospital of Stomatology, China Medical University, Shenyang, China.

Bone is an active tissue, being constantly renewed in healthy individuals with participation of the immune system to a large extent. Any imbalance between the processes of bone formation and bone resorption is linked to various inflammatory bone diseases. The immune system plays an important role in tissue formation and bone resorption. Recently, many studies have demonstrated complex interactions between the immune and skeletal systems. Both of immune cells and cytokines contribute to the regulation of bone homeostasis, and bone cells, including osteoblasts, osteoclasts, osteocytes, also influence the cellular functions of immune cells. These crosstalk mechanisms between the bone and immune system finally emerged, forming a new field of research called osteoimmunology. Therefore, the immune microenvironment is crucial in determining the speed and outcome of bone healing, repair, and regeneration. In this review, we summarise the role of the immune microenvironment in bone regeneration from the aspects of immune cells and immune cytokines. The elucidation of immune mechanisms involved in the process of bone regeneration would provide new therapeutic targets for improving the curative effects of bone injury treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijms.61080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579305PMC
September 2021

The Prevalence of Hypertension in the Population without Awareness of the Disease: Data from a Rural Town of Shandong Province, China.

Int J Hypertens 2021 3;2021:9672994. Epub 2021 Nov 3.

TEDA International Cardiovascular Hospital, Tianjin 300457, China.

Objective: To understand the prevalence of hypertension in the population without awareness of hypertension in a rural area, a cross-sectional study was carried out.

Methods: Blood pressures were measured in residents over 60 years during the National Basic Public Health Service project carrying on in a rural town of Shandong province. Combined with detail information of the hypertension disease history, the status of prevalence of hypertension was calculated. Basic information and clinical laboratory examinations were analyzed with Student' or ' or chi-square test for univariate analysis. Multinomial logistic analysis was used in exploring multiple variables.

Results: According to the individual history and blood pressure levels, the awareness rate of hypertension in the population over 60 years old was 50.3% (1285/2554, 95% CI: 48.3-52.3%). The prevalence of hypertension was 55.1% (1270/2304, 95% CI: 53.1-57.2%) in the population without awareness of hypertension, in which the proportion of stage 1 hypertension was 58.8% (747/1270), stage 2 hypertension was 28.9% (367/1270), and stage 3 hypertension was 12.3% (156/1270). The prevalence of hypertension in men was 54.4% (611/1124, 95% CI: 51.4-57.3%), which was almost the same as that in women (55.8%, 659/1180, 95% CI: 53.0-58.7%) (  = 0.515, =0.473). The prevalence of hypertension increased with age (  = 11.848, =0.001). Age, BMI, total cholesterol, triglyceride, and drinking rate were positively correlated with the prevalence of hypertension, that is, the higher the level of these factors, the higher the prevalence of hypertension; on the contrary, LDL and smoking rate might be negatively correlated with the prevalence of hypertension, which means, the higher the prevalence of hypertension, the lower the level of these two indicators. Binary and multinominal logistic results showed that age, BMI, and drinking had stronger effects on the higher blood pressure level.

Conclusions: The awareness rate of hypertension among the elderly in a rural area needs to be further improved. In the prevention and control of hypertension, close attention should be paid to the group of elder, high BMI index, high levels of total cholesterol and triglyceride, and drinking habits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9672994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580620PMC
November 2021

A Study of Zero Anaphora Resolution in Chinese Discourse: From the Perspective of Psycholinguistics.

Front Psychol 2021 28;12:663168. Epub 2021 Oct 28.

School of Interpreting and Translation Studies, Guangdong University of Foreign Studies, Guangzhou, China.

Anaphora is the main linguistic means to establish discourse coherence, and anaphora resolution is the psychological process to maintain this coherence. Chinese discourse is characterized with providing multiplicity of linguistic clues to readers by employing various referential apparatuses such as pronoun anaphora, zero anaphora, and so on. As a way of avoiding repeated reference to an object that is mentioned beforehand, zero anaphora is frequently employed in discourse. The production and resolution of zero anaphora largely concerns some constraints underlying psychological mechanisms. We particularly focus on zero anaphora resolution in the present study to try to discover some specific aspects of the underlying mechanism, hoping to find out some factors unique to the resolution process. We designed the first two experiments to probe into the psychological reality when participants were presented with sentences containing either pronoun anaphora or zero anaphora or both under discourse condition with topic continuity in Experiment 1a and topic discontinuity in Experiment 1b. We did not find any significant difference in the reaction time between zero anaphora resolution and pronoun anaphora resolution, indicating that zero anaphora possibly works within the processing mechanism on which pronoun anaphora resolution depends. However, we found significantly longer time in reading the first sentence in any of the discourse, showing that the first-mention effect exists in anaphora resolution. We further explored the time course of zero anaphora resolution by measuring the reaction time during the period when participants read sentences that varied according to the location where zero anaphora occurred under two conditions: topic continuity (Experiment 2a) vs. topic discontinuity (Experiment 2b). The strategies of searching for the referential information were found divergent: the exhaustive searching strategy was adopted when the topics within a discourse were kept continuous and the heuristic searching strategy was employed when the topics were discontinuous. The design of Experiment 5 took the factor of voice type and situation consistency into consideration, investigating in what way do those factors influence the resolution of zero anaphora. The voice type, according to the results, plays a significant role for its exclusively close relationship with the first-mention effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.663168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581763PMC
October 2021

In vitro and in vivo Activity of Combinations of Polymyxin B with Other Antimicrobials Against Carbapenem-Resistant .

Infect Drug Resist 2021 5;14:4657-4666. Epub 2021 Nov 5.

Department of Infectious Disease, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.

Purpose: To study the in vitro and in vivo antibacterial activities of polymyxin B (PB) and other five antimicrobial agents, including imipenem (IMP), meropenem (MEM), tigecycline (TGC), sulbactam (SUL), and rifampicin (RIF), alone or in combination against carbapenem-resistant (CRAB).

Methods: Microbroth dilution method was used to determine the minimum inhibitory concentration (MIC) of ten strains of CRAB against six antibacterial drugs, and the checkerboard method was used to determine the fractional inhibitory concentration index (FICI). A mouse pneumonia model was established by intranasal instillation of Ab5075 to evaluate the antibacterial activity in vivo.

Results: The resistance rate of ten CRAB strains to IMP, MEM, and SUL was 100%, that to PB and TGC was 0%, and that to RIF was 20%. When PB was used in combination with the other five antibiotics in vitro, it mainly showed synergistic and additive effects on CRAB. The synergistic effect of PB and RIF was maximal, followed by MEM and IMP but was weak with SUL and TGC. In vivo, compared to the model group (untreated with antibiotics), treatment group (six antibiotics alone and PB combined with the other five antibiotics) reduced the bacterial load in the lung tissue and the serum inflammatory factors (IL-1β, IL-6, and TNF-α). The bacterial load and the inflammatory factors of the combined group decreased significantly than that of the single group (<0.05). The IL-6 and TNF-α values of the PB combined with the RIF group were significantly lower than the two drugs used individually.

Conclusion: The combination of PB and IMP, MEM, and RIF exerted robust in vitro synergistic effects on CRAB isolates. The combination of PB and the other five antimicrobial agents had a better effect in the mouse pneumonia model than single agent, while the combination of PB and RIF had the best effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S334200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577563PMC
November 2021

Cancer drug screening with an on-chip multi-drug dispenser in digital microfluidics.

Lab Chip 2021 Nov 11. Epub 2021 Nov 11.

State Key Laboratory of Analog and Mixed-Signal VLSI, Institute of Microelectronics, University of Macau, Macau, China.

Microfluidics has been the most promising platform for drug screening with a limited number of cells. However, convenient on-chip preparation of a wide range of drug concentrations remains a large challenge and has restricted wide acceptance of microfluidics in precision medicine. In this paper, we report a digital microfluidic system with an innovative control structure and chip design for on-chip drug dispensing to generate concentrations that span three to four orders of magnitude, enabling single drug or combinatorial multi-drug screening with simple electronic control. Specifically, we utilize droplet ejection from a drug drop sitting on a special electrode, named a drug dispenser, under high-voltage pulse actuation to deliver the desired amount of drugs to be picked up by a cell suspension drop driven by low-voltage sine wave actuation. Our proof-of-principle validation for this technique as a convenient single and multi-drug screening involved testing of the drug toxicity of two chemotherapeutics, cisplatin (Cis) and epirubicin (EP), towards MDA-MB-231 breast cancer cells and MCF-10A normal breast cells. The results are consistent with those screened based on traditional 96-well plates. These findings demonstrate the reliability of the drug screening system with an on-chip drug dispenser. This system with fewer cancer cells, less drug consumption, a small footprint, and high scalability with regard to concentration could pave the way for drug screening on biopsied primary tumor cells for precision medicine or any concentration-related research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1lc00895aDOI Listing
November 2021

Predictive Value of Preoperative Profiling of Serum Metabolites for Emergence Agitation After General Anesthesia in Adult Patients.

Front Mol Biosci 2021 21;8:739227. Epub 2021 Oct 21.

Department of Anesthesiology, Peking University Third Hospital, Beijing, China.

Emergence agitation (EA) in adult patients under general anesthesia leads to increased postoperative complications and heavy medical burden. Unfortunately, its pathogenesis has not been clarified until now. The purpose of the present study was to explore the relationship between preoperative serum metabolites and EA. We used an untargeted metabolic analysis method to investigate the different metabolomes in the serum of EA patients and non-EA patients undergoing elective surgical procedures after the induction of general anesthesia. A Richmond Agitation-Sedation Scale score ≥ +2 was diagnosed as EA during postoperative emergence. Non-EA patients were matched with EA patients according to general characteristics. Preoperative serum samples of the two groups were collected to investigate the association between serum metabolites and EA development. The serum samples of 16 EA patients with 34 matched non-EA patients were obtained for metabolic analysis. After screening and alignment with databases, 31 altered metabolites were detected between the two groups. These metabolites were mainly involved in the metabolism of lipids, purines, and amino acids. Analyses of receiver-operating characteristic curves showed that the preoperative alterations of choline, cytidine, glycerophosphocholine, L-phenylalanine, oleamide, and inosine may be associated with adult EA. Multiple metabolic abnormalities (including those for lipids, purines, and amino acids) and other pathological processes (e.g., neurotransmitter imbalance and oxidative stress) may contribute to EA. Several altered metabolites in serum before surgery may have predictive value for EA diagnosis. This study might afford new metabolic clues for the understanding of EA pathogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmolb.2021.739227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566542PMC
October 2021

Genetic variations for the eggshell crystal structure revealed by genome-wide association study in chickens.

BMC Genomics 2021 Nov 2;22(1):786. Epub 2021 Nov 2.

National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, 100193, Beijing, China.

Background: Eggshell is a bio-ceramic material comprising columnar calcite (CaCO) crystals and organic proteinaceous matrix. The size, shape and orientation of the CaCO crystals influence the microstructural properties of chicken eggshells. However, the genetic architecture underlying eggshell crystal polymorphism remains to be elucidated.

Results: The integral intensity of the nine major diffraction peaks, total integral intensity and degree of orientation of the crystals were measured followed by a genome-wide association study in 839 F2 hens. The results showed that the total integral intensity was positively correlated with the eggshell strength, eggshell thickness, eggshell weight, mammillary layer thickness and effective layer thickness. The SNP-based heritabilities of total integral intensity and degree of orientation were 0.23 and 0.06, respectively. The 621 SNPs located in the range from 55.6 to 69.1 Mb in GGA1 were significantly associated with TA. PLCZ1, ABCC9, ITPR2, KCNJ8, CACNA1C and IAPP, which are involved in the biological process of regulating cytosolic calcium ion concentration, can be suggested as key genes regulating the total integral intensity.

Conclusions: The findings greatly advance the understanding of the genetic basis underlying the crystal ultrastructure of eggshell quality and thus will have practical significance in breeding programs for improving eggshell quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-021-08103-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565016PMC
November 2021

An improved experimental method for simultaneously isolating hepatocytes and hepatic stellate cells in mouse liver infected with Echinococcus multilocularis.

J Clin Lab Anal 2021 Nov 1:e24084. Epub 2021 Nov 1.

State Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

Background: Alveolar echinococcosis (AE) is a zoonotic disease caused by the larval stage of Echinococcus multilocularis parasitizing in the human liver, causing local pathological changes in the liver and manifesting as hyperplasia, liver fibrosis, atrophy, degeneration, and necrosis. Here, we report a method that can simultaneously isolate hepatocytes and hepatic stellate cells (HSCs) from mice infected with Echinococcus multilocularis.

Methods: A mouse model of AE was established. Hepatocytes and HSCs were isolated from mouse liver using a two-step method combining in situ collagenase perfusion and gradient centrifugation. Expressions of Alb, Desmin, and α-SMA were detected with immunofluorescence to identify the isolated hepatocytes and HSCs.

Results: The viability and purity of hepatocytes and HSCs both reached 90% or above. For hepatocytes, clear cell boundaries were observed, and the nuclei were round or oval, with clear nucleoli. There was a homogeneous distribution of the hepatocyte marker Alb in the cytoplasm of hepatocytes. Lipid droplets and Desmin expression were observed in the cytoplasm of freshly isolated HSCs. During the activation of HSCs, the lipid droplets gradually decreased and disappeared with a high expression of α-SMA.

Conclusion: Hepatocytes and HSCs are simultaneously isolated. This may provide a research tool to investigate the interaction between hepatocytes and HSCs and to investigate the mechanism of Echinococcus multilocularis infection-induced liver pathological changes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcla.24084DOI Listing
November 2021

Corrigendum: Upregulated LINC01667 Expression Is Correlated With Poor Prognosis in Hepatocellular Carcinoma.

Front Oncol 2021 15;11:785394. Epub 2021 Oct 15.

State Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

[This corrects the article DOI: 10.3389/fonc.2021.650173.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.785394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555468PMC
October 2021

Activation of cannabinoid receptor 2 alleviates glucocorticoid-induced osteonecrosis of femoral head with osteogenesis and maintenance of blood supply.

Cell Death Dis 2021 10 30;12(11):1035. Epub 2021 Oct 30.

Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

In glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH), downregulated osteogenic ability and damaged blood supply are two key pathogenic mechanisms. Studies suggested that cannabinoid receptor 2 (CB2) is expressed in bone tissue and it plays a positive role in osteogenesis. However, whether CB2 could enhance bone formation and blood supply in GC-induced ONFH remains unknown. In this study, we focused on the effect of CB2 in GC-induced ONFH and possible mechanisms in vitro and in vivo. By using GC-induced ONFH rat model, rat-bone mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) to address the interaction of CB2 in vitro and in vivo, we evaluate the osteogenic and angiogenic effect variation and possible mechanisms. Micro-CT, histological staining, angiography, calcein labeling, Alizarin red staining (ARS), alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) staining, TUNEL staining, migration assay, scratch assay, and tube formation were applied in this study. Our results showed that selective activation of CB2 alleviates GC-induced ONFH. The activation of CB2 strengthened the osteogenic activity of BMSCs under the influence of GCs by promotion of GSK-3β/β-catenin signaling pathway. Furthermore, CB2 promoted HUVECs migration and tube-forming capacities. Our findings indicated that CB2 may serve as a rational new treatment strategy against GC-induced ONFH by osteogenesis activation and maintenance of blood supply.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-04313-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556843PMC
October 2021

The gelling behavior of gellan in the presence of different sodium salts.

Int J Biol Macromol 2021 Dec 28;193(Pt A):768-777. Epub 2021 Oct 28.

School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

It is well known that metal ions have great effects on gelling behaviors of gellan aqueous systems, however, the effects of their co-ions - anions have rarely been studied. Herein, we investigated the effects of four kinds of sodium salts with different anions (NaCl, CHCOONa, NaCO and NaCHO) on gelling behaviors of gellan aqueous systems in terms of gelling temperature and gel hardness. It was found that, when [Na] was low (20 mM), the salt with Cl or CHCOO favored the gelling of gellan aqueous systems, while the salt with CO or CHO took adverse effects probably because CO or CHO could react with divalent cations (Ca and Mg) in gellan to form precipitates or chelates and break their interactions with gellan (salt bridges). When [Na] was high (50 or 80 mM), all the four kinds of salts facilitated gelling due to the shielding effects of high concentrations of Na on the negative charges along the gellan chains, and followed the order of: Cl > CHCOO > CO > CHO. This study demonstrates the effects of anion kind of salts on gelling behaviors of gellan aqueous systems and provides references for the application of gellan.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.10.173DOI Listing
December 2021

Biomechanical Motion-Activated Endogenous Wound Healing through LBL Self-Powered Nanocomposite Repairer with pH-Responsive Anti-Inflammatory Effect.

Small 2021 Oct 28:e2103997. Epub 2021 Oct 28.

State Key Laboratory of Bioelectronics & National Demonstration Centre for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China.

Wound care is still worthy of concern, and effective measures such as electrical stimulating therapy (EST) have sparked compellingly for wound repair. Especially, portable and point-of-care EST devices get extremely desired but these are often limited by inevitable external power sources, lack of biological functions, and mechanical properties conforming to skin tissue. Herein, a dress-on-person self-powered nanocomposite bioactive repairer of wound is designed. As such, the cooperation of the film prepared by layer-by-layer self-assembling 2-hydroxypropyltrimethyl ammonium chloride chitosan (HTCC), alginate (ALG), and poly-dopamine/Fe nanoparticles (PFNs), with a self-powered nanogenerator (SN) driven by motion into a nanocomposite repairer (HAP/SN-NR) is conducted. The HAP/SN-NR not only guides cell behavior (proliferation and migration rate ≈61.7%, ≈52.3%), but also facilitates neovascularization (enhanced CD31 expression >4-fold) through its self-powered EST, and the endogenous wound closure with no inflammatory in rats owing to reactive oxygen species (ROS)-clearance of HAP/SN-NR in vitro/vivo through responsively releasing poly-dopamine nanoparticles at wound pH. Enormous efforts illustrate that the repairer is endowed with high self-adhesion to tissue, self-healing, and biodegradation, accelerating wound healing (50% closure ≈5 days). This strategy sheds light on novel multifunctional portable sensor-type dressings and propels the development of intelligent medical devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202103997DOI Listing
October 2021

Radioactive Seed Strand Efficacy in Superior Vena Cava Stenting Due to Non-small-cell Lung Cancer Obstruction.

Vasc Endovascular Surg 2021 Oct 27:15385744211054300. Epub 2021 Oct 27.

Department of Radiology, Xuzhou Central Hospital, Xuzhou, China.

Purpose: This study aims to determine the clinical effectiveness of a stent with radioactive seed strand (RSS) inserted in patients with superior vena cava (SVC) obstruction (SVCO) secondary to non-small-cell lung cancer (NSCLC).

Methods: Between January 2013 and December 2019, 63 patients with SVCO related to NSCLC received stent implantation with (n = 30) or without (n = 33) RSS insertion at our center. The clinical efficacy, stent patency duration, and overall survival (OS) were compared between these two groups.

Results: Both groups achieved 100% clinical and technical success rates. There were no obstacles associated with the procedure performed for the patients. Two patients in the RSS group and 7 patients in the stent-alone group experienced stent re-stenosis. The rate of re-stenosis between the two groups was not significantly different ( = .099). Patients in the RSS group had significantly longer median patency than those in the stent-alone group (381 vs 309 days, = .045). All patients died because of the development of tumors during the follow-up. Patients in the RSS group had a significantly longer median OS than those in the stent-alone group (229 vs 178 days, = .026). During the follow-up, no patient in the RSS group suffered RSS migration or brachytherapy-related complications.

Conclusion: For patients with SVCO secondary to NSCLC, a stent with RSS insertion is efficacious and safe, and it may improve stent patency and OS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/15385744211054300DOI Listing
October 2021

Impact of the gut microecology on Campylobacter presence revealed by comparisons of the gut microbiota from chickens raised on litter or in individual cages.

BMC Microbiol 2021 10 22;21(1):290. Epub 2021 Oct 22.

Poultry Science Laboratory, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.

Background: Poultry is the major reservoir of Campylobacter that contributes to human campylobacteriosis and threatens food safety. Litter contact has been linked to Campylobacter colonization, but the gut microecological impact underlying this link remains not fully clear. Here, we sought to investigate the impact of the gut microecology on the presence of Campylobacter by examining the microbiota in the duodenum, jejunum, ileum, ceca, and feces from chickens raised on commercial litter and in individual cages at 0-57 days of age.

Results: Through litter contact, the presence of Campylobacter was found to benefit from microecological competition among Lactobacillus, Helicobacter, and genera that are halotolerant and aerobic or facultatively anaerobic in the upper intestine, such as Corynebacterium and Brachybacterium. The presence was also promoted by the increased abundance in obligate anaerobic fermentation microbes, especially members of the orders Clostridiales and Bacteroidales. The longitudinal analysis supported the vertical or pseudo-vertical transmission but suggested that colonization might occur immensely at 7-28 days of age. We observed a host genetic effect on the gut microecology, which might lead to increased heterogeneity of the microecological impact on Campylobacter colonization.

Conclusions: The findings advance the understanding of the gut microecological impact on Campylobacter presence in the chicken gut under conditions of litter contact and suggest that manipulations of the gut microecology, as well as the microbes identified in the Campylobacter association networks, might be important for the development of intervention strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12866-021-02353-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532315PMC
October 2021

Identification of Antimicrobial Peptide Genes in Black Rockfish and Their Responsive Mechanisms to Infection.

Biology (Basel) 2021 Oct 9;10(10). Epub 2021 Oct 9.

School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.

The black rockfish, , is a typical viviparous teleost, which belongs to the family Scorpaenidae. Due to its high economic and ecological values, has been widely cultured in East Asian countries. With the enlargement of cultivation scale, bacterial and viral diseases have become the main threats to the farming industry of , which have resulted in significant economic losses. In this study, Illumina shotgun sequencing, single-molecule real-time (SMRT) sequencing, 10× genomics and high-throughput chromosome conformation capture (Hi-C) technologies were collectively applied to assemble the genome of . Then, we identified the antimicrobial peptide genes (AMPs) in the genome. In total, 214 AMPs were identified in the genome, which can be divided into 33 classes according to the annotation and cataloging of the Antimicrobial Peptides Database (APD3). Among these AMPs, thrombin-derived C-terminal peptide (TCP) was the dominant type, followed by RegIIIgamma and chemokine. The amino acid sequences of the TCP, cgUbiquitin, RegIIIalpha, RegIIIgamma, chemokine shared 32.55%, 42.63%, 29.87%, 28.09%, and 32.15% similarities among the same type in Meanwhile, the expression patterns of these AMPs in nine healthy tissues and at different infection time points in intestine were investigated. The results showed that the numbers and types of AMPs that responded to infection gradually increased as the infection progressed. In addition, we analyzed the phylogenetic relationships of hepcidins in teleost. The identification of AMPs based on the whole genome could provide a comprehensive database of potential AMPs, and benefit for the understanding of the molecular mechanisms of immune responses to infection in . This would further offer insights into an accurate and effective design and development of AMP for aquaculture therapy in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biology10101015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533284PMC
October 2021

Bone marrow mesenchymal stem cell-derivedexosomal miR-34c-5p ameliorates RIF by inhibitingthe core fucosylation of multiple proteins.

Mol Ther 2021 Oct 19. Epub 2021 Oct 19.

Department of Nephrology, The First Affiliated Hospital of Dalian Medical University, Key Laboratory of Kidney Disease of Liaoning Province, The Center for the Transformation Medicine of Kidney Disease of Liaoning Province, No. 222 Zhongshan Road, Dalian 116011, China. Electronic address:

Renal interstitial fibrosis (RIF) is an incurable pathological lesion in chronic kidney diseases. Pericyte activation is the major pathological characteristic of RIF. Fibroblast and macrophage activation are also involved in RIF. Studies have revealed that core fucosylation (CF), an important post-translational modification of proteins, plays a key role in pericyte activation and RIF by regulating multiple profibrotic signaling pathways as a hub-like target. Here, we reveal that mesenchymal stem cell (MSC)-derived exosomes reside specifically in the injured kidney and deliver microRNA (miR)-34c-5p to reduce cellular activation and RIF by inhibiting CF. Furthermore, we showed that the CD81-epidermal growth factor receptor (EGFR) ligand-receptor complex aids the entry of exosomal miR-34c-5p into pericytes, fibroblasts, and macrophages. Altogether, our findings reveal a novel role of MSC-derived exosomes in inhibiting multicellular activation via CF and provide a potential intervention strategy for renal fibrosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ymthe.2021.10.012DOI Listing
October 2021

Characterization of the Durum Wheat- 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance.

Front Plant Sci 2021 30;12:745290. Epub 2021 Sep 30.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Durum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT23 is used as the female parent in crosses with its AB genome donor Lanmai. A tetraploid line YL-443 with supernumerary spikelets and high resistance to stripe rust was selected out from the pentaploid F progeny. Somatic analysis using multicolor fluorescence hybridization (mc-FISH) revealed that this line is a disomic substitution line with the 4B chromosome pair of Lanmai replaced by the 4D chromosome pair of AT23. Comparing with Lanmai, YL-443 shows an increase in the number of spikelets and florets per spike by 36.3 and 75.9%, respectively. The stripe rust resistance gene carried on the 4D chromosome was fully expressed in the tetraploid background. The present 4D(4B) disomic substitution line YL-443 was distinguished from the previously reported 4D(4B) lines with the 4D chromosomes from Chinese Spring (CS). Our study demonstrated that YL-443 can be used as elite germplasm for durum wheat breeding targeting high yield potential and stripe rust resistance. The -specific PCR marker and the 4D chromosome-specific KASP markers together with its unique features of pubescent leaf sheath and auricles can be utilized for assisting selection in breeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.745290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514839PMC
September 2021

Potential Serum Biomarkers for Postoperative Neurocognitive Disorders Based on Proteomic Analysis of Cognitive-Related Brain Regions.

Front Aging Neurosci 2021 29;13:741263. Epub 2021 Sep 29.

Department of Anesthesiology, Peking University Third Hospital, Beijing, China.

Postoperative neurocognitive disorders (po-NCD), including postoperative delirium (POD) and delayed neurocognitive recovery (dNCR), are common in geriatric surgical patients. However, the ideal diagnostic biomarkers to predict individual risks of po-NCDs have not been identified. In this study, proteomic analysis was used to detect dysregulated proteins in three cognitive-related brain regions, the hippocampus, prefrontal cortex, and temporal lobe, of aged dNCR rats. The common affected proteins in these three brain regions were further verified by real-time polymerase chain reaction and western blotting. Furthermore, serum samples from aged rats with dNCR and elderly hip fracture patients with POD were also assessed with enzyme linked immunosorbent assays to investigate the biomarker potential of these dysregulated proteins. The increased expression levels of haptoglobin, caseinolytic protease (ClpP), and alpha-2 macroglobulin (A2M) as well as decreased expression levels of 14-3-3β/α and biliverdin reductase-A (BVR-A) were validated by proteomic analysis in the hippocampus, prefrontal cortex, and temporal lobe of aged dNCR rats. The increased expression of haptoglobin and decreased expression of 14-3-3β/α were further demonstrated in the three brain regions by western blotting. Moreover, increased levels of S100A6 and BVR-A in the hippocampus, S100A6 in the prefrontal cortex, and A2M in the temporal lobe were also observed. More intriguingly, both decreased serum 14-3-3β/α and increased A2M in geriatric POD patients as well as decreased serum ClpP in aged dNCR rats were verified. These results not only indicate potential diagnostic biomarkers for po-NCD but also provide directions for further pathological investigations. www.ClinicalTrials.gov, identifier [ChiCTR1900027393].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnagi.2021.741263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511679PMC
September 2021

Chinese Color Nest Project : An accelerated longitudinal brain-mind cohort.

Dev Cogn Neurosci 2021 Oct 11;52:101020. Epub 2021 Oct 11.

Research Center for Lifespan Development of Mind and Brain, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China; State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China; Developmental Population Neuroscience Research Center, International Data Group/McGovern Institute for Brain Research, Beijing Normal University, Beijing 100875, China. Electronic address:

The ongoing Chinese Color Nest Project (CCNP) was established to create normative charts for brain structure and function across the human lifespan, and link age-related changes in brain imaging measures to psychological assessments of behavior, cognition, and emotion using an accelerated longitudinal design. In the initial stage, CCNP aims to recruit 1520 healthy individuals (6-90 years), which comprises three phases: developing (devCCNP: 6-18 years, N = 480), maturing (matCCNP: 20-60 years, N = 560) and aging (ageCCNP: 60-84 years, N = 480). In this paper, we present an overview of the devCCNP, including study design, participants, data collection and preliminary findings. The devCCNP has acquired data with three repeated measurements from 2013 to 2017 in Southwest University, Chongqing, China (CCNP-SWU, N = 201). It has been accumulating baseline data since July 2018 and the second wave data since September 2020 in Chinese Academy of Sciences, Beijing, China (CCNP-CAS, N = 168). Each participant in devCCNP was followed up for 2.5 years at 1.25-year intervals. The devCCNP obtained longitudinal neuroimaging, biophysical, social, behavioral and cognitive data via MRI, parent- and self-reported questionnaires, behavioral assessments, and computer tasks. Additionally, data were collected on children's learning, daily life and emotional states during the COVID-19 pandemic in 2020. We address data harmonization across the two sites and demonstrated its promise of characterizing the growth curves for the overall brain morphometry using multi-center longitudinal data. CCNP data will be shared via the National Science Data Bank and requests for further information on collaboration and data sharing are encouraged.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dcn.2021.101020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517840PMC
October 2021

A study of the development path of key disciplines in the Guang'an hospital based on construction of west China's compact medical consortium hospitals.

Am J Transl Res 2021 15;13(9):10469-10476. Epub 2021 Sep 15.

Institute of Hospital Management, West China Hospital of Sichuan University Chengdu 610041, Sichuan, China.

Objective: To investigate the development path of key disciplines in Guang'an Hospital based on the construction of West China's compact medical consortium hospitals.

Methods: The urological medical staff and facilities in Guang'an Hospital were selected as the study subjects. The urological medical staff were interviewed, and a detailed investigation and analysis of the current development of urology and the allocation of experts and medical equipment and resources were performed, so as to promote the development of key disciplines in Guang'an Hospital.

Results: We aim to focus on the operational targets of the compact medical consortium while remaining committed to the construction of disciplines of urology. Meanwhile, the relationship between patients and health care providers was coordinated in regard to health care services. The mean of compactness of the dimensions involved in the targets of the operational body of patients was 4.71, while that of the improvement of the medical and health environment was 4.88. The allocated proportion of healthcare resources was optimized, and the operational efficiency was improved. If an excellent medical service experience was provided, the obtained data are statistically significant.

Conclusion: Based on the medical consortium, the assessment model of scientific experimental methods, and with the goal of improving the quality of medical treatment regarding urology, the responsibilities of urologists are further defined. A standardized training plan was conducted, with assessment systems of disciplines and better access systems of urologists are formulated and improved, and therefore the construction of key disciplines is promoted, thereby providing more references for the construction and development of key disciplines of urology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507059PMC
September 2021

Isolation of 2 simazine-degrading bacteria and development of a microbial agent for bioremediation of simazine pollution.

An Acad Bras Cienc 2021 6;93(suppl 3):e20210373. Epub 2021 Oct 6.

Pudong Agrotechnology Extension Center, No. 386, Pailou Road, 201201, Shanghai, China.

Simazine was one of the most commonly used herbicides and was widely used to control broadleaf weeds in agriculture and forestry. Its widespread use had caused wide public concern for its high ecological toxicity. In order to remove simazine residues, 2 strains capable of effectively degrading simazine were isolated from the soil and named SIMA-N5 and SIMA-N9. SIMA-N5 was identified as Bacillus licheniformis by 16SrRNA sequence analysis, and SIMA-N9 was Bacillus altitudinis. According to the degradation ratio of simazine in a certain period of time, the degradation ability of different strains was evaluated. The degradation efficiency of simazine (5 mg/L) by SIMA-N9 could reach about 98% in 5d, and the strain SIMA-N5 could reach 94% under the same conditions. In addition, the addition of Pennisetum rhizosphere soil during the process of degrading simazine by strain SIMA-N9 could effectively improve the degradation efficiency. The strain SIMA-N9 has been developed as a microbial agent for the bioremediation of simazine contamination in soil. The new microbial agent developed by using SIMA-N9 has achieved satisfactory application effects. Based on the research results already obtained in this study, it was considered that strain SIMA-N9 and its live bacterial agent could play an important role in bioremediation of simazine pollution. This study could not only provide a set of solutions to the simazine pollution, but also provide a reference for the treatment of other pesticide pollution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/0001-3765202120210373DOI Listing
October 2021

Three chromosome-level duck genome assemblies provide insights into genomic variation during domestication.

Nat Commun 2021 10 11;12(1):5932. Epub 2021 Oct 11.

National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA; College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Rd, Beijing, 100193, China.

Domestic ducks are raised for meat, eggs and feather down, and almost all varieties are descended from the Mallard (Anas platyrhynchos). Here, we report chromosome-level high-quality genome assemblies for meat and laying duck breeds, and the Mallard. Our new genomic databases contain annotations for thousands of new protein-coding genes and recover a major percentage of the presumed "missing genes" in birds. We obtain the entire genomic sequences for the C-type lectin (CTL) family members that regulate eggshell biomineralization. Our population and comparative genomics analyses provide more than 36 million sequence variants between duck populations. Furthermore, a mutant cell line allows confirmation of the predicted anti-adipogenic function of NR2F2 in the duck, and uncovered mutations specific to Pekin duck that potentially affect adipose deposition. Our study provides insights into avian evolution and the genetics of oviparity, and will be a rich resource for the future genetic improvement of commercial traits in the duck.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-26272-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505442PMC
October 2021

Stem cells from exfoliated deciduous teeth transplantation ameliorates Sjögren's syndrome by secreting soluble PD-L1.

J Leukoc Biol 2021 Oct 8. Epub 2021 Oct 8.

Department of Pediatric Dentistry, School and Hospital of Stomatology, China Medical University, Shenyang, China.

Mesenchymal stem cell transplantation (MSCT) regulates immune cells, and is a promising therapeutic approach for treating autoimmune diseases. Stem cells from human exfoliated deciduous teeth (SHED) are a unique postnatal stem cell population from the cranial neural crest with high self-renewal, multipotent differentiation, and superior immunomodulatory properties. However, the mechanisms by which SHED can treat autoimmune diseases remain unclear. Sjögren's syndrome (SS) is an autoimmune disease histologically characterized by high lymphocytic infiltration in the salivary and lacrimal glands that results in dryness symptoms. This study explores the potential of systemic transplantation of SHED to ameliorate SS-induced dryness symptoms in mice. Overall, SHED could rescue the balance of regulatory T cell (Treg)/T helper cell 17 (Th17) in the recipient SS mice. Mechanistically, SHED promoted Treg conversion and inhibited Th17 function via paracrine effects, which were related to the secretion of soluble programmed cell death ligand 1 (sPD-L1). Moreover, it directly induced Th17 apoptosis via cell-cell contact, leading to the up-regulation of Treg and down-regulation of Th17 cells. In summary, SHED-mediated rescue of Treg/Th17 balance via the sPD-L1/PD-1 pathway ameliorates the gland inflammation and dryness symptoms in SS mice. These findings suggest that SHED are a promising stem cell source for the treatment of autoimmune diseases in the clinical setting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/JLB.6MA0921-752RRDOI Listing
October 2021
-->