Publications by authors named "Ning Yan"

367 Publications

An air-stable, reusable Ni@Ni(OH) nanocatalyst for CO/bicarbonate hydrogenation to formate.

Nanoscale 2021 May 6. Epub 2021 May 6.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.

Production of formate via CO2/bicarbonate hydrogenation using cheap metal-based heterogeneous catalysts is attractive. Herein, we report the organometallic synthesis of a foam-like Ni@Ni(OH)2 composite nanomaterial which exhibited remarkable air stability and over 2 times higher catalytic activity than commercial RANEY® Ni catalyst in formate synthesis. Formate generation was achieved with an optimal rate of 6.0 mmol gcat-1 h-1 at 100 °C, a significantly lower operation temperature compared to the 200-260 °C reported in the literature. Deep characterization evidenced that this nanomaterial was made of an amorphous Ni(OH)2 phase covering metallic Ni sites; a core-shell structure which is crucial for the stability of the catalyst. The adsorption of bicarbonates onto the Ni@Ni(OH)2 catalyst was found to be a kinetically relevant step in the reaction, and the Ni-Ni(OH)2 interface was found to be beneficial for both CO2 and H2 activation thanks to a cooperative effect. Our findings emphasize the underestimated potential of Ni-based catalysts in CO2 hydrogenation to formate, indicating a viable strategy to develop stable, cheap metal catalysts for greener catalytic applications.
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http://dx.doi.org/10.1039/d1nr01054aDOI Listing
May 2021

Comparison of Phenolic and Flavonoid Compound Profiles and Antioxidant and α-Glucosidase Inhibition Properties of Cultivated Soybean () and Wild Soybean ().

Plants (Basel) 2021 Apr 20;10(4). Epub 2021 Apr 20.

Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.

Wild soybean ( Sieb.et WS) has been used as a traditional food in China for many years and contains significantly higher levels of isoflavones than cultivated soybean (; CS), but the secondary metabolites, including flavonoids and the phenolic composition differences between them, remain unclear. The results showed that WS possessed significantly higher total phenolic and flavonoid content and exhibited better antioxidant and α-glucosidase inhibition activities as well as excellent protective effects against HO-induced oxidative injury in a human endothelial cell line. Through metabolomic analysis, 642 metabolites were identified, and 238 showed differential expression, with 151 upregulated and 87 downregulated. A total of 79 flavonoid compounds were identified, 42 of which were upregulated in WS. 2'-Hydroxygenistein, garbanzol, protocatechuic aldehyde, ligustilide, and resveratrol were the most discriminated compounds in WS. The metabolic pathway analysis of differential metabolites related to the biosynthesis of flavonoids and phenolic acids were the biosynthesis of phenylpropanoids, flavonoids, isoflavonoids, flavones, and flavonols. This study substantially elucidated differences in the content of flavonoids and biological activities between WS and CS, which is useful information for the effective utilization of these two black soybean species in food processing.
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http://dx.doi.org/10.3390/plants10040813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074397PMC
April 2021

Proton-Assisted Reconstruction of Perovskite Oxides: Toward Improved Electrocatalytic Activity.

ACS Appl Mater Interfaces 2021 May 28;13(18):22009-22016. Epub 2021 Apr 28.

School of Physics and Technology, Wuhan University, Wuhan 430072, China.

Electrocatalysis is indispensable to various emerging energy conversion and storage devices such as fuel cells and water electrolyzers. Owing to their unique physicochemical properties, perovskite oxide materials are one of the most promising water oxidation (OER) catalysts solely comprising earth-abundant elements. Nonetheless, many perovskite oxide catalysts suffer from a number of inherent problems such as the A-site cation segregation on the surface, coarse particles due to agglomeration/sintering, and surface decomposition during catalytic reactions. Besides, the catalytic activity is often incomparable with those of the state-of-the-art catalysts. In this work, we developed a proton-assisted approach to mitigate these common challenges. The protonation via the interaction of oxygen vacancies and water molecules induced the formation of protonic defects and the lattice expansion of the perovskite, leading to the fracture of big particles to yield small nanoparticles. This hydration in an acidic solution also selectively removed the A-site cation segregates and generated a spinel/perovskite heterostructure on the surface. We verified this approach using three typical perovskite OER catalysts including BaSrCoFeO (BSCF), LaSrCoFeO (LSCF), and LaSrMnO (LSM). The processed catalysts showed much improved activity while maintaining their excellent stability, surpassing most of today's OER catalysts based on complex oxides.
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http://dx.doi.org/10.1021/acsami.1c03276DOI Listing
May 2021

LncRNA PlncRNA-1 accelerates the progression of prostate cancer by regulating PTEN/Akt axis.

Aging (Albany NY) 2021 Apr 13;13(8):12113-12128. Epub 2021 Apr 13.

Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.

Long non-coding RNAs are key regulators of tumor development and progression, with the potential to be biomarkers of tumors. This study aimed to explore the role of PlncRNA-1 in the progression of prostate cancer (PCa). We found that PlncRNA-1 was up-regulated in 85.29% of PCa tissues and could predict the T stage of PCa patients to a certain extent. Results showed that inhibition of PlncRNA-1 expression potentially promoted cell apoptosis, suppressed the proliferation, migration, and invasion of cells, and triggered G2/M cycle arrest and . PlncRNA-1 was mainly localized in the nucleus and PlncRNA-1 expression and phosphatase and tensin homologue (PTEN) expression were negatively correlated. Mechanistically, knockdown of PlncRNA-1 increased expression levels of PTEN protein and phosphorylated PTEN protein, and decreased expression levels of Akt protein and phosphorylated Akt protein. Rescue experiments demonstrated that PTEN inhibitors abolished the changes in PTEN/Akt pathway caused by PlncRNA-1 interference. PlncRNA-1 can promote the occurrence and development of PCa via the PTEN/Akt pathway. PlncRNA-1 may, therefore, be a new candidate target for the treatment of PCa.
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http://dx.doi.org/10.18632/aging.202919DOI Listing
April 2021

Recovery of the nitrifying ability of acclimated biomass exposed to para-nitrophenol.

Sci Total Environ 2021 Mar 24;781:146697. Epub 2021 Mar 24.

Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ 85287-5701, USA.

Para-nitrophenol (PNP) is often detected in industrial wastewater that is discharged into municipal wastewater treatment plants. Intermittent discharge of PNP into municipal treatment facilities puts their biological process at risk of inhibition, and the risk is especially great for nitrification. In this work, nitrifying biomass was acclimated to PNP. The acclimated biomass retained most of its ammonium-removal activity when it was exposed to PNP at up to 100 mg/L, while the normal (unacclimated) biomass had nearly complete inhibition. PNP was effectively biodegraded by the acclimated biomass, but the normal biomass had minimal PNP biodegradation. After PNP disappeared, the acclimated biomass recovered its ability for NH-N removals within one to two days, but the normal biomass did not fully recovery even after seven days. The acclimated biomass had superior ability to sustain nitrification due to its ability to biodegrade PNP and its selection of nitrifying bacteria more resistant to PNP. The PNP-acclimated community was enriched in genera that could have been active in the biodegradation of PNP, such as Chloroflexi. Although the abundance of well-known nitrifiers, Nitrosomonas and Nitrospira, decreased, Nitrosospira and other genera within the Proetobacteria phylum increased, presumably because they were more resistant to PNP.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146697DOI Listing
March 2021

Circadian variations in the occurrence of first-ever intracerebral hemorrhage from different sources of income: a hospital-based cross-sectional study.

BMC Neurol 2021 Mar 31;21(1):140. Epub 2021 Mar 31.

Department of Neurology, University-Town Hospital of Chongqing Medical University, University-Town Middle Road 55, 401331, Chongqing, China.

Background: The onset time of intracerebral hemorrhage (ICH) may be closely related to the working style and living habits of people, which are determined by different income sources in China. Therefore, the purpose of our study was to investigate the Circadian Variations in the occurrence of ICH from different sources of income.

Methods: This retrospective study enrolled 4,327 patients with first-ever ICH. Based on the time of day at which the patients developed symptoms, the classifiable onset time was assigned to one of eight three-hour intervals. And based on different income sources, they were categorized into three groups: Farmers, Wage-earners, and Freelancers. Demographic and risk factors of patients were then summarized, and the circadian variation of the 3 groups of patients' known time of onset and those stratified by sex and age were analyzed.

Results: The frequency of ICH onset exhibited significant circadian variation among the 3 income groups, demonstrating a bimodal distribution in the daytime, with a nadir during the night (all P < 0.001). Three groups showed a significant initial peak between 06:01 and 09:00, and the same peak was observed in their subgroups of sex and age. In the 3 income source groups, there was a smaller second peak that between 15:01 and 18:00 for Farmers and Wage-earners and 18:01 and 21:00 for Freelancers. After stratification by sex and age, the second peak was between 18:01 and 21:00 for female in Farmers, female in Freelancers, under 65 years of age in Wage-earners and 65 years or older in Freelancers, while 15:01 and 18:00 for the other groups.

Conclusions: Different circadian variations of ICH onset time are found in patients with different income sources in southwest China's Chongqing Municipality cohort. Moreover, the frequency and distribution pattern of peak hours may be closely related to the working style and living habits of people with different income sources.
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http://dx.doi.org/10.1186/s12883-021-02163-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010953PMC
March 2021

Evaluation and revision of core postoperative nursing outcomes for laryngeal carcinoma in China.

BMC Nurs 2021 Mar 23;20(1):51. Epub 2021 Mar 23.

Nursing College of Shanxi Medical University, Taiyuan City, Shanxi, China.

Background: The core nursing outcomes for laryngeal carcinoma in China needed further screening and revision. This study aimed to evaluate and revise a questionnaire according to the "Core Nursing Outcomes for Otorhinolaryngology Head-Neck" of the Nursing Outcomes Classification (NOC, 5th Edition), and determine suitable postoperative nursing outcomes for patients with laryngeal carcinoma in China.

Methods: The commonly used postoperative nursing outcomes for laryngeal carcinoma were screened using a questionnaire given to 93 nurses. An initial expert consultation questionnaire was constructed to discuss the indicators for each nursing outcome. A total of 20 experts were identified using the Delphi method, and their recommendations and revisions on the selected nursing outcomes were collected.

Results: A total of 14 postoperative core nursing outcomes and 69 indicators were identified for postoperative patients with laryngeal carcinoma, which are subordinate to 4 domains of the NOC: "Physiologic Health", "Psychosocial Health", "Health Knowledge & Behavior", and "Perceived Health".

Conclusions: The screening and revision of the NOC outcomes and indicators of the Delphi method could be applied to assess the effect of nursing intervention and the quality of the nursing service in China.
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http://dx.doi.org/10.1186/s12912-021-00569-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989408PMC
March 2021

Characteristics of denitrification in a vertical baffled bioreactor.

Environ Res 2021 Mar 18;197:111046. Epub 2021 Mar 18.

Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ, 85287-5701, USA.

A vertical baffled bioreactor (VBBR) was employed for tertiary denitrification. Its features were designed to minimize the demand for externally supplied electron donor by minimizing net biomass synthesis and oxygen respiration. Over a two-year period, complete denitrification was realized routinely in the VBBR. The nitrate-removal rate was proportion to the influent COD/N ratio, with complete denitrification possible for COD/N ratios >3 gCOD/gN. Batch kinetic tests carried out at the end of years 1 and 2 documented that supplied electron donor was oxidized in the first 1-2 h, but nitrate and nitrite reductions occurred predominantly after 2 h and were driven by internally stored electron donor. Measurements confirmed that the VBBR minimized the demand of added electron donor: The observed yield was only 0.05 mgVSS/mgCOD, and the COD demand for O respiration was only 1-6.7% of the COD demand for N reductions. Biofilm samples taken from the upper and lower ports in cylinder of VBBR had similarly high alpha diversity and dominant genera, but the upper biofilm had a denitrification rate about 70% greater than the lower biofilm. The higher denitrification rate in the upper biofilm correlated its higher content of active biomass.
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http://dx.doi.org/10.1016/j.envres.2021.111046DOI Listing
March 2021

Simultaneous phase-shifting interferometer with a monitored spatial light modulator flexible reference mirror.

Appl Opt 2021 Feb;60(6):1550-1557

A simultaneous phase-shifting interferometer with a monitored spatial light modulator (SLM) flexible reference mirror is proposed to balance the flexibility and accuracy of aspheric-surface in-process measurements. In this method, polarization simultaneous phase-shifting camera systems are applied to reduce the influence of environmental vibrations on the in-process measurements. An SLM reference mirror is employed to improve the flexibility of in-process measurements. A device is integrated to monitor the SLM surface in order to improve measurement accuracy caused by the spatial phase nonuniformity and modulation instability of the SLM. Thus, the SLM surface is monitored and the aspheric surface is measured simultaneously in only one interferometer, which presents the advantages of a compact structure and simple calibration. A flat acrylic mirror with an unknown surface figure error is measured by the proposed interferometer. Cross tests demonstrate the feasibility of this interferometer.
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http://dx.doi.org/10.1364/AO.414810DOI Listing
February 2021

Stachydrine inhibits TGF-β1-induced epithelial-mesenchymal transition in hepatocellular carcinoma cells through the TGF-β/Smad and PI3K/Akt/mTOR signaling pathways.

Anticancer Drugs 2021 Mar 5. Epub 2021 Mar 5.

Department of Hepatology Department of Preventive Treatment, Xi'an Hospital of Traditional Chinese Medicine, Xi'an, China.

Stachydrine is a bioactive alkaloid that has been found to exert tumor-suppressive potential. However, the effect of stachydrine on hepatocellular carcinoma (HCC) has not been previously investigated. In the present study, we investigated the effect of transforming growth factor-β1 (TGF-β1)-induced epithelial-mesenchymal transition (EMT) in HepG2 cells. Our results showed that stachydrine significantly suppressed TGF-β1-induced HepG2 cell migration and invasion in a dose-dependent manner. Stachydrine prevented TGF-β1-induced EMT in HepG2 cells, as proved by the increased expression level of E-cadherin and decreased expression levels of N-cadherin and vimentin. In addition, stachydrine attenuated TGF-β1-induced upregulation of TGF-β receptor I (TβRI) in both protein and mRNA levels. Further mechanism investigations proved that stachydrine prevented TGF-β1-induced activation of Smad2/3 and phosphoinositol-3-kinase (PI3K)/Akt/mTOR signaling pathways in HepG2 cells. In conclusion, these findings demonstrated that stachydrine prevented TGF-β1-induced EMT in HCC cells through Smad2/3 and PI3K/Akt/mTOR signaling pathways. Thus, stachydrine might be a potential therapeutic agent for the treatment of HCC.
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http://dx.doi.org/10.1097/CAD.0000000000001066DOI Listing
March 2021

Downregulated mRNA Expression of ZNF385B Is an Independent Predictor of Breast Cancer.

Int J Genomics 2021 3;2021:4301802. Epub 2021 Feb 3.

Department of Anesthesia, The Second Hospital of Jilin University, Changchun, Jilin 130022, China.

Background: ZNF385B, a zinc finger protein, has been known as a potential biomarker in some neurological and hematological studies recently. Although numerous studies have demonstrated the potential function of zinc finger proteins in tumor progression, the effects of ZNF385B in breast cancer (BC) are less studied.

Methods: The Oncomine database and "ESurv" tool were used to explore the differential expression of ZNF385B in pan-cancer. Furthermore, data of patients with BC were downloaded from The Cancer Genome Atlas (TCGA). The receiver operating characteristic (ROC) curve of ZNF385B expression was established to explore the diagnostic value of ZNF385B and to obtain the cut-off value of high or low ZNF385B expression in BC. The chi-square test as well as Fisher exact test was used for identification of the relationships between clinical features and ZNF385B expression. Furthermore, the effects of ZNF385B on BC patients' survival were evaluated by the Kaplan-Meier and Cox regression. Data from the Gene Expression Omnibus (GEO) database were employed to validate the results of TCGA. Protein expression of ZNF385B in BC patient specimens was detected by immunohistochemistry (IHC) staining.

Results: ZNF385B expression was downregulated in most types of cancer including BC. Low ZNF385B expression was related with survival status, overall survival (OS), and recurrence of BC. ZNF385B had modest diagnostic value, which is indicated by the area under the ROC curve (AUC = 0.671). Patients with lower ZNF385B expression had shorter OS and RFS (relapse-free survival). It had been demonstrated that low ZNF385B expression represented independent prognostic value for OS and RFS by multivariate survival analysis. The similar results were verified by datasets from the GEO database as well. The protein expression of ZNF385B was decreased in patients' samples compared with adjacent tissues by IHC.

Conclusions: Low ZNF385B expression was an independent predictor for worse prognosis of BC patients.
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http://dx.doi.org/10.1155/2021/4301802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876827PMC
February 2021

Synergy of strains that accelerate biodegradation of pyridine and quinoline.

J Environ Manage 2021 May 10;285:112119. Epub 2021 Feb 10.

Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ85287-5701, USA.

Three bacterial strains were isolated from activated sludge acclimated to biodegrade pyridine and quinoline simultaneously. The strains were identified as Bacillus tropicus, Bacillus aquimaris, and Rhodococcus ruber. When the isolated bacteria were used for pyridine and quinoline biodegradation in separate or combined modes, R. ruber had much faster kinetics, and combining R. ruber with one or both of the Bacillus strains increased further the biodegradation kinetics. For example, the time needed for complete biodegradation of 1 mM quinoline and pyridine decreased to 20 h and 6 h, respectively, with the three strains combined, compared to 26 h and 7 h with R. ruber alone. Whereas quinoline was completely mineralized by all three strains, 10-14% of the pyridine persisted as a dead-end product, 2-hydroxypyridine (2HP). The acclimated sludge from which the three bacterial species were isolated was able to transform 2HP, and adding the bacterial strains (especially R. ruber) to the acclimated sludge accelerated the rate of 2HP removal and mineralization through a form of synergy.
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http://dx.doi.org/10.1016/j.jenvman.2021.112119DOI Listing
May 2021

Expanding the Boundary of Biorefinery: Organonitrogen Chemicals from Biomass.

Acc Chem Res 2021 04 12;54(7):1711-1722. Epub 2021 Feb 12.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 117585, Singapore.

Organonitrogen chemicals are essential in many aspects of modern life. Over 80% of the top 200 prescribed pharmaceutical products contain at least one nitrogen atom in the molecule, while all top 10 agrochemicals contain nitrogen, just to name a few. At present, the prevailing industrial processes for manufacturing organonitrogen chemicals start from nonrenewable fossil resources, but eventually we have to make these chemicals in a more sustainable manner. Biomass represents the largest renewable carbon resource on earth, which is inexpensive and widely available. Integrating biomass into the organonitrogen chemical supply chain will mitigate the carbon footprint, diversify the product stream, and enhance the economic competitiveness of biorefinery. Short-cut synthesis routes can be created for oxygen-containing organonitrogen compounds by exploiting the inherent oxygen functionalities in the biomass resources. Moreover, for nitrogen-containing biomass components such as chitin, a unique opportunity to make organonitrogen chemicals bypassing the energy-intensive Haber-Bosch ammonia synthesis process arises. Estimated at 100 billion tons of annual production in the world, chitin captures more nitrogen than the Haber-Bosch process in the form of amide functional groups in its polymer side chain.In this Account, we intend to summarize our efforts to establish new reaction routes to synthesize valuable organonitrogen chemicals from renewable resources. Enabled by tailor-designed catalytic systems, diverse nitrogen-containing products including amines, amino acids, nitriles, and -heterocycles have been obtained from a range of biomass feedstock either directly or via intermediate platform compounds. Two strategies to produce organonitrogen chemicals are presented. For platform chemicals derived from cellulose, hemicellulose, lignin, and lipids, which are enriched with oxygen functionalities, in particular, hydroxyl groups, the key chemistry to be developed is the catalytic transformation of hydroxyl groups into nitrogen-containing groups using NH as the nitrogen source. Along this line, Ru- and Ni-based heterogeneous catalysts are developed to convert alcohols to amines and/or nitriles via a thermal catalytic pathway, while CdS nanomaterials are explored to promote -OH to -NH conversion under visible-light irradiation. Metal-zeolite multifunctional systems are further established to enable the synthesis of -heterocycles from -heterocycles. The second strategy involves the use of chitin and chitin derivatives as the starting materials. Under the concept of shell biorefinery, distinctive protocols have been established to chemically transform chitin as the sole feedstock to amino sugars, amino alcohols, furanic amides, and -heterocycles. By combining mechanochemistry with biotransformation, an integrated process to convert shrimp shell waste to complex, high-value, chiral compounds including tyrosine and l-DOPA is also demonstrated.
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http://dx.doi.org/10.1021/acs.accounts.0c00842DOI Listing
April 2021

Multiple hydrogen bonding self-assembly tailored electrospun polyimide hybrid filter for efficient air pollution control.

J Hazard Mater 2021 Jun 29;412:125260. Epub 2021 Jan 29.

College of Bioresources Chemical and Materials Engineering, Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, National Demonstration Center for Experimental Light Chemistry Engineering Education, Key Laboratory of Paper Based Functional Materials of China National Light Industry, Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science & Technology, Xi'an 710021, China. Electronic address:

Air pollutions are extremely serious threats to human health and the functional hybrid filter is able to remove complicated pollutants with great potential. However, the stable structure design of hybrid filter to provide efficient filtration and adsorption performance for high temperature applications still remains a challenge. In this study, electrospun polyimide (PI) based hybrid filter was fabricated via multiple hydrogen bonding self-assembly for high-temperature air purification. In particular, Octa(amino-propylsilsesquioxane) (POSS-NH) was utilized as "bridge" for the surface activation of PI fiber, and then amino-functionalized Zeolitic Imidazolate Framework-8 (NH-ZIF-8) nanocrystals were anchored on the fiber surface through hydrogen bonding. On account of the synergistic effect of the interception effect of fibers and the electrostatic interaction of NH-ZIF-8 nanocrystals, the as-obtained PI-POSS@ZIF hybrid filter possessed excellent filtration performance with a high PM0.3 removal efficiency of 99.28% and a low pressure drop of 49.21 Pa at high temperature of 280 °C. Moreover, due to the massive micropore structure, rich open metal sites and functional groups of NH-ZIF-8, the hybrid filter exhibited prominent VOCs adsorption performance with adsorption capability of 89.95 mg/g for formaldehyde.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125260DOI Listing
June 2021

Transformation of Corn Lignin into Sun Cream Ingredients.

ChemSusChem 2021 Mar 16;14(6):1586-1594. Epub 2021 Feb 16.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.

Production of value-added chemicals from lignocellulose is one of the key strands of the emerging bio-economy. A synthetic approach has been developed to produce p-methoxy coumarate esters from waste corn stover and corn bran, and further transform these compounds into consumer products. In the first step, p-coumaric acid (pCA) and ferulic acid (FA) are released through mild alkaline hydrolysis of corn waste, in more than 20 mg g yield (10 wt % based on lignin content). Subsequently, heterogeneous solid acids catalyze the esterification of pCA with various alcohols. Amberlite IR-15 shows the best catalytic performance, owing to its optimal acid density, large surface area, and suitable pore diameter. The solid acid catalyst can be recycled with slight activity loss and regenerated by acid washing and vacuum drying. Finally, methylation of p-coumarate ester to give target product p-methoxy coumarate esters is carried out by using commercially available and low-cost inorganic bases (e. g., K CO ). Base-catalyzed transesterification of methyl p-methoxy coumarate to p-methoxy coumarate esters is also investigated.
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http://dx.doi.org/10.1002/cssc.202002739DOI Listing
March 2021

The Adsorption Ability of NCFM to Benzo(a)pyrene in PM.

J Toxicol 2021 7;2021:6290524. Epub 2021 Jan 7.

College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, China.

The objective of this work was to explore the ability of lactic acid bacteria strains to bind benzo(a)pyrene (B(a)P) existing in PM. In this study, we examined the ability of NCFM to bind B(a)P in the simulated PM environment. Among the tested 5 strains, NCFM exhibited the best capacity to bind B(a)P, and its B(a)P binding percentage was 60.00%. Simulations of organic and inorganic systems which represent PM indicated that B(a)P could be absorbed by strain NCFM. For the inorganic system of pH 5, NCFM bound 92.74% B(a)P with a cell concentration of 1 × 10 cfu/mL at 37°C for 8 hr. Regarding the organic system with pH 6, 73.00% B(a)P was bound by strain NCFM after this bacterium was incubated at 37°C for 10 min. A quick B(a)P binding by this probiotic bacterium took place in the organic system. The removal of B(a)P from PM was significantly related to incubation time, cultivation temperature, pH, and cell concentration. Thus, our finding shows that long-term consumption of NCFM is beneficial for the reduction of B(a)P towards the population who are exposed to PM, although the ability of this bacterium to adsorb B(a)P is partly affected by the differences in the origin of PM.
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http://dx.doi.org/10.1155/2021/6290524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808799PMC
January 2021

More rapid dechlorination of 2,4-dichlorophenol using acclimated bacteria.

Bioresour Technol 2021 Apr 20;326:124738. Epub 2021 Jan 20.

Biodesign Swette Center for Environmental Biotechnology, Arizona State University, 85287-5701, USA.

The key step for anaerobic biodegradation of 2,4-dichlorophenol (2,4-DCP) is an initial dechlorination reaction, but Cl in the para-position is more difficult to remove than Cl in the ortho-position using normal 2,4-DCP-acclimated bacteria. In this work, a bacterial community previously acclimated to biodegrading 2,4-DCP slowly dechlorinated 4-chlorophenol (4-CP Cl only in the para-position), which limited mineralization. That community was exposed to the selective pressure of having 4-CP as its only organic substrate in order to generate a 4-CP-dechlorinating community. When the 4-CP-dechlorinating community was challenged with 2,4-DCP, 4-CP hardly accumulated, although the kinetics for 2,4-DCP biodegradation were slower. When the community acclimated to 4-CP was mixed with the community acclimated to 2,4-DCP, the 2,4-DCP removal rate remained high, and 4-CP was more rapidly biodegraded. The genera Treponema, Blvii28, Dechloromonas, Nitrospira, and Thauera were significantly more abundant in the 4-CP-dechlorinating biomass and may have played roles in 2,4-DCP dechlorination and mineralization.
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http://dx.doi.org/10.1016/j.biortech.2021.124738DOI Listing
April 2021

Modeling and Analysis of System Error for Highly Curved Freeform Surface Measurement by Noncontact Dual-Axis Rotary Scanning.

Sensors (Basel) 2021 Jan 14;21(2). Epub 2021 Jan 14.

State Key Laboratory of Precision Measuring Technology & Instruments, Laboratory of Micro/Nano Manufacturing Technology, Tianjin University, Tianjin 300072, China.

Profile measurement is a key technical enabler in the manufacturing of highly curved freeform surfaces due to their complex geometrical shape. A current optical probe was used to measure nearly rotary freeform surfaces with the help of one rotation axis, because the probe needs to measure along the normal vector of the surface under the limitation of the numerical aperture (NA). This kind of measuring system generally has a high cost due to the high-precision, multi-axis platform. In this paper, we propose a low-cost, dual-axis rotation scanning method for a highly curved freeform surface with an arbitrary shape. The optical probe can scan the surface profile while always keeping consistent with the normal vector of the measuring points with the help of the double rotation axis. This method can adapt to the changes in curvature in any direction for a highly curved freeform surface. In addition, the proposed method provides a system error calibration technique for the rotation axis errors. This technique can be used to avoid the dependence of the measuring system on the high-precision platform. The three key system errors that affect the measurement accuracy such as installation error of the B-axis, A-axis, and XZ perpendicularity error are first analyzed through establishing an error model. Then, the real error values are obtained by the optimal calculation in the calibration process. Finally, the feasibility of the measurement method is verified by measuring one cone mirror and an F-theta mirror and comparing the results to those obtained using commercial equipment. The maximum measurable angle of the system is ±90°, the maximum measurable diameter is 100 mm, and the measurement accuracy of the system reaches the micron level in this paper.
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http://dx.doi.org/10.3390/s21020554DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829850PMC
January 2021

Different Benzendicarboxylate-Directed Structural Variations and Properties of Four New Porous Cd(II)-Pyridyl-Triazole Coordination Polymers.

Front Chem 2020 17;8:616468. Epub 2020 Dec 17.

College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, China.

Four new different porous crystalline Cd(II)-based coordination polymers (CPs), i. e., [Cd(mdpt)]·2HO (), [Cd(mdpt)(-bdc)(HO)] (), [Cd(Hmdpt)(-bdc)]·2HO (), and [Cd(mdpt)(bpdc)]·2.5NMP (), were obtained successfully by the assembly of Cd(II) ions and bitopic 3-(3-methyl-2-pyridyl)-5-(4-pyridyl)-1,2,4-triazole (Hmdpt) in the presence of various benzendicarboxylate ligands, i.e., 1,3/1,4-benzenedicarboxylic acid (-Hbdc, -Hbdc) and biphenyl-4,4'-bicarboxylate (Hbpdc). Herein, complex is a porous 2-fold interpenetrated four-connected 3D topological framework based on the mdpt ligand; reveals a two-dimensional (2D) network. Interestingly, presents a three-dimensional (3D) rare interpenetrated double-insertion supramolecular net 2D ···ABAB··· layers and can be viewed as an topological net, while complex displays a 3D framework. Then, the different gas sorption performances were carried out carefully for complexes and , the results of which showed has preferable sorption than that of and can be the potential CO storage and separation material. Furthermore, the stability and luminescence of four complexes were performed carefully in the solid state.
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http://dx.doi.org/10.3389/fchem.2020.616468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773847PMC
December 2020

Surgical outcome and risk factors for cervical spinal cord injury patients in chronic stage: a 2-year follow-up study.

Eur Spine J 2021 Jan 2. Epub 2021 Jan 2.

Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, China.

Purpose: This study aims to assess the nerve function deficient recovery in surgically treated patients with cervical trauma with spinal cord injury (SCI) in chronic stage and figure out prognostic predictors of improvement in impairment and function.

Methods: We reviewed the clinical and radiological data of 143 cervical SCI patients in chronic stage and divided into non-operative group (n = 61) and operative group (n = 82). The severity of neurological involvement was assessed using the ASIA motor score (AMS) and Functional Independence Measure Motor Score (FIM MS). The health-related quality of life was measured using the SF-36 questionnaire. Correspondence between the clinical and radiological findings and the neurological outcome was investigated.

Results: At 2-year follow-up, surgery resulted in greater improvement in AMS and FIM MS than non-operative group. Regression analysis revealed that lower initial AMS (P = 0.000), longer duration after injury (P = 0.022) and injury above C4 level (P = 0.022) were factors predictive of lower final AMS. Longer duration (P = 0.020) and injury above C4 level (P = 0.010) were associated with a lower FIM MS. SF-36 scores were significantly lower in higher age (P = 0.015), female patients (P = 0.009) and patients with longer duration (P = 0.001).

Conclusion: It is reasonable to consider surgical decompression in patients with cervical SCI in chronic stage and persistent spinal cord compression and/or gross cervical instability. Initial AMS, longer duration, injury above C4 level, higher age and female patients are the five major relevant factors of functional recovery.
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http://dx.doi.org/10.1007/s00586-020-06703-1DOI Listing
January 2021

Integrin αvβ6 cooperates with resiquimod to restore antigen-specific immune tolerance in airway allergy.

Immunol Lett 2021 Feb 29;230:49-58. Epub 2020 Dec 29.

Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Shenzhen, China; Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China. Electronic address:

Background: Integrin αvβ6 can convert the transforming growth factor (TGF)-β precursor to the mature form. Resiquimod (R848) can generate TGF-β-producing regulatory T cells (Treg). Thus, to concurrent administration of specific antigen and R848 may generate antigen-specific Tregs, that is expected to restore immune tolerance in subjects with airway allergic diseases (AAD).

Methods: A bio-nanoparticle, designated Rexo, containing an antigen/MHC II complex and R848, was naturally assembled in dendritic cells, that was released as an exosome. An AAD mouse model was developed used to test the effects of Rexo on restoring the immune tolerance in the airways.

Results: Exposure to R848 failed to induce Tregs in the β6-deficient mouse airway tissues, that were successfully induced in wild type mice. The results were validated inin vitro experiments. R848 activated the TLR7/MyD88/p38 signal pathway to increase the αvβ6 levels in CD4 T cells, the αvβ6 then converted the TGF-β precursor to its mature form, and thus, induced Treg generation. Administration of Rexo restored the antigen-specific immune tolerance in the airways manifesting efficiently suppressing experimental AAD by inducing antigen-specific Tregs in the airways and inhibiting antigen-specific Th2 response.

Conclusions: Rexos can inhibit experimental AAD via inducing antigen-specific Tregs to restore immune tolerance in the airway tissues, suggesting that Rexos have the translational potential to be used in the treatment of AAD.
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http://dx.doi.org/10.1016/j.imlet.2020.12.011DOI Listing
February 2021

[Expressions of CXCL8, CXCL10 and Th1 /Th2 cytokines in the serum and cerebrospinal fluid of patients with neurosyphilis].

Zhonghua Nan Ke Xue 2020 Apr;26(4):335-340

Department of Dermatovenerology, Jiangsu Provincial People's Hospital / The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Objective: To investigate the levels of chemokines 8 and 10 (CXCL8 and CXCL10), Th1 cytokines (IL-2, IL-12 and IFN-γ) and Th2 cytokines (IL-6 and IL-10) in the serum and cerebrospinal fluid of patients with neurosyphilis and elucidate their roles in the immune response and pathogenesis of neurosyphilis.

Methods: Using ELISA, we detected the expressions of CXCL8, CXCL10, IL2, IL-2, IFN-γ, IL-6 and IL-10 in the serum and cerebrospinal fluid of 42 cases of neurosyphilis, 44 cases of syphilis and 40 cases of non-inflammatory diseases of the nervous system (the control group).

Results: The serum levels of CXCL8, CXCL10, IL-2, IL-12, IFN-γ, IL-6 and IL-10 were significantly lower in the neurosyphilis group than in the syphilis and control groups (P < 0.05), and so were they in the male than in the female neurosyphilis patients (P < 0.05). However, the expressions of CXCL8, CXCL10, IL-2, IL-12, IFN-γ, IL-6 and IL-10 in the cerebrospinal fluid were remarkably higher in the neurosyphilis group than in the syphilis and control groups (P < 0.05), and so were they in the male than in the female neurosyphilis patients (P < 0.05).

Conclusions: Patients with neurosyphilis have cellular immune dysfunction, and their immune response involves CXCL8, CXCL10 and Th1 / Th2 cytokines.
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April 2020

Wntless (Wls): A Prognostic Index for Progression and Patient Survival of Breast Cancer.

Onco Targets Ther 2020 8;13:12649-12659. Epub 2020 Dec 8.

Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, People's Republic of China.

Background: Wntless (Wls) is an essential protein that is necessary for the secretion of Wnt proteins. While numerous researches have demonstrated that aberrations in Wnt/β-catenin expression lead to tumorigenesis and progression in many cancer types, the effects of Wls in breast cancer (BC) are less studied.

Methods: The mRNA and protein expression of Wls in BC cell lines were detected by RT-qPCR and Western blot; the protein expression of patient samples was detected by immunohistochemistry (IHC). The associations between Wls expression and clinicopathological factors as well as survival time, including overall survival (OS) and disease-free survival (DFS) were analyzed. Bioinformatics analysis was used to reveal the correlation between Wls genes and associated genes or pathways.

Results: Wls was overexpressed in BC cell lines and tissues. The expression level of Wls was significantly correlated with tumor size, estrogen receptor (ER), progesterone receptor (PR), Ki-67, molecular classification, and follow-up status. Spearman correlation analysis showed that Wls protein expression was negatively correlated with ER and PR, which was confirmed by bioinformatics analysis in mRNA level. However, there were positive relationships with MBNG (modified Black's nuclear grade), tumor size, Ki-67, molecular classification, follow-up, and vital status. Univariate and multivariate analysis showed that Wls was an independent prognostic factor for OS and DFS in BC patients. Moreover, Wls was a significant prognostic indicator of OS and DFS in a hormone receptor-positive (HR+) subgroup. GSEA showed that estrogen and androgen response, as well as epithelial-mesenchymal transition pathways, were up-regulated in the Wls high-expression group.

Conclusion: Overexpression of Wls is a significant marker of worse prognosis in BC and might play a crucial role in the HR+ subgroup.
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http://dx.doi.org/10.2147/OTT.S265324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737487PMC
December 2020

Towards the Circular Economy: Converting Aromatic Plastic Waste Back to Arenes over a Ru/Nb O Catalyst.

Angew Chem Int Ed Engl 2021 Mar 18;60(10):5527-5535. Epub 2021 Jan 18.

Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.

The upgrading of plastic waste is one of the grand challenges for the 21 century owing to its disruptive impact on the environment. Here, we show the first example of the upgrading of various aromatic plastic wastes with C-O and/or C-C linkages to arenes (75-85 % yield) via catalytic hydrogenolysis over a Ru/Nb O catalyst. This catalyst not only allows the selective conversion of single-component aromatic plastic, and more importantly, enables the simultaneous conversion of a mixture of aromatic plastic to arenes. The excellent performance is attributed to unique features including: (1) the small sized Ru clusters on Nb O , which prevent the adsorption of aromatic ring and its hydrogenation; (2) the strong oxygen affinity of NbO species for C-O bond activation and Brønsted acid sites for C-C bond activation. This study offers a catalytic path to integrate aromatic plastic waste back into the supply chain of plastic production under the context of circular economy.
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http://dx.doi.org/10.1002/anie.202011063DOI Listing
March 2021

Growing vertical aligned mesoporous silica thin film on nanoporous substrate for enhanced degradation, drug delivery and bioactivity.

Bioact Mater 2021 May 10;6(5):1452-1463. Epub 2020 Nov 10.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032, China.

Mesoporous silica thin film has been widely used in various fields, particularly the medical implant coating for drug delivery. However, some drawbacks remain with the films produced by traditional method (evaporation-induced self-assembly, EISA), such as the poor permeability caused by their horizontal aligned mesochannels. In this study, the vertical aligned mesoporous silica thin film (VMSTF) is uniformly grown alongside the walls of titania nanotubes array via a biphase stratification growth method, resulting in a hierarchical two-layered nanotubular structure. Due to the exposure of opened mesopores, VMSTF exhibits more appealing performances, including rapid degradation, efficient small-molecular drug (dexamethasone) loading and release, enhanced early adhesion and osteogenic differentiation of MC3T3-E1 cells. This is the first time successfully depositing VMSTF on nanoporous substrate and our findings suggest that the VMSTF may be a promising candidate for bone implant surface coating to obtain bioactive performances.
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http://dx.doi.org/10.1016/j.bioactmat.2020.10.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670213PMC
May 2021

Lignin Cellulose Nanofibrils as an Electrochemically Functional Component for High-Performance and Flexible Supercapacitor Electrodes.

ChemSusChem 2021 Feb 9;14(4):1057-1067. Epub 2020 Dec 9.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada.

The increasing demand for wearable electronics has driven the development of supercapacitor electrode materials toward enhanced energy density, while being mechanically strong, flexible, as well as environmentally friendly and low-cost. Taking advantage of faradaic reaction of quinone groups in natural lignin that is covalently bound to the high-strength cellulose nanofibrils, the fabrication of a novel class of mechanically strong and flexible thin film electrodes with high energy storage performance is reported. The electrodes were made by growing polyaniline (PANI) on flexible films composed of lignin-containing cellulose nanofibrils (LCNF) and reduced graphene oxide (rGO) nanosheets at various loading levels. The highest specific capacitance was observed for the LCNF/rGO/PANI electrode with 20 wt% rGO nanosheets (475 F g at 10 mV s and 733 F g at 1 mV s ), which represented a 68 % improvement as compared to a similar electrode made without lignin. In addition, the LCNF/rGO(20)/PANI electrode demonstrated high rate performance and cycle life (87 % after 5000 cycles). These results indicated that LCNF functioned as an electrochemically active multifunctional component to impart the composite electrode with mechanical strength and flexibility and enhanced overall energy storage performance. LCNF/rGO(20)/PANI electrode was further integrated in a flexible supercapacitor device, revealing the excellent promise of LCNF for fabrication of advanced flexible electrodes with reduced cost and environmental footprint and enhanced mechanical and energy storage performances.
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http://dx.doi.org/10.1002/cssc.202002558DOI Listing
February 2021

Comparison of the contents of phenolic compounds including flavonoids and antioxidant activity of rice (Oryza sativa) and Chinese wild rice (Zizania latifolia).

Food Chem 2021 May 11;344:128600. Epub 2020 Nov 11.

Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China. Electronic address:

The contents of phenolic compounds, especially flavonoids, and antioxidant activity of rice (Oryza sativa, Os) and Chinese wild rice (Zizania latifolia, Zl) harvested in China were compared. Zl possessed significantly higher contents of total phenolics, flavonoids, and proanthocyanidins and exhibited higher antioxidant activity than in the Os Xian group, the Os Geng group, and red rice. The flavonoid contents of Os and Zl were compared using a UHPLC-QqQ-MS-based metabolomics approach. A total of 159 flavonoids were identified, among which 78 showed differential expression (72 up-regulated and six down-regulated in the Zl group). The Kyoto Encyclopaedia of Genes and Genomes annotation and classification indicated that the differentially expressed flavonoids were mainly related to anthocyanin biosynthesis. Moreover, candidate genes for flavonoid biosynthesis in Os and Zl were identified in this study. Compared with non-pigmented and red rice, Zl may be more nutritious and is thus considered a better source of natural antioxidants.
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http://dx.doi.org/10.1016/j.foodchem.2020.128600DOI Listing
May 2021

Observing Single-Atom Catalytic Sites During Reactions with Electrospray Ionization Mass Spectrometry.

Angew Chem Int Ed Engl 2021 02 29;60(9):4764-4773. Epub 2020 Dec 29.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 1 Engineering Drive 3, 117580, Singapore, Singapore.

Single-atom catalysts (SACs) have become a prominent theme in heterogeneous catalysis, not least because of the potential fundamental insight into active sites. The desired level of understanding, however, is prohibited due to the inhomogeneity of most supported SACs and the lack of suitable tools for structure-activity correlation studies with atomic resolution. Herein, we describe the potency of electrospray ionization mass spectrometry (ESI-MS) to study molecularly defined SACs supported on polyoxometalates in catalytic reactions. We identified the exact composition of active sites and their evolution in the catalytic cycle during CO and alcohol oxidation reactions performed in the liquid phase. Critical information on metal-dependent reaction mechanisms, the key intermediates, the dynamics of active sites and even the stepwise activation barriers were obtained, which would be challenging to gather via prevailingly adopted techniques in SAC research. DFT calculations revealed intricate details of the reaction mechanisms, and strong synergies between ESI-MS defined SAC sites and electronic structure theory calculations become apparent.
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http://dx.doi.org/10.1002/anie.202011632DOI Listing
February 2021

Lignin-Based Polyurethane: Recent Advances and Future Perspectives.

Macromol Rapid Commun 2021 Feb 18;42(3):e2000492. Epub 2020 Nov 18.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, M5S 3B3, Canada.

Polyurethane (PU), as a polymer material with versatile product forms and excellent performance, is used in coatings, elastomers, adhesives, and foams widely. However, the raw materials (polyols and isocyanates) of PU are usually made using petroleum-derived chemicals. With the concern for depletion of petroleum resources and the associated negative impact on the environment, developing technologies that can use renewable raw materials as feedstock has become a research hotspot. Lignin, as an abundant, natural, and renewable organic carbon resource, has been explored as raw material for making polyurethanes because it possesses rich hydroxyl groups on its surface. Meanwhile, compared to vegetable oils, lignin does not compete with food supply and performance of the resulting products is superior. Lignin or modified lignin has been shown to impart the polyurethane material with additional functionalities, such as UV-blocking ability, hydrophobicity, and flame retardancy. However, the utilization of lignin has encountered some challenges, such as product isolation, heterogeneity, aggregation, steric hindrance, and low activity. This paper summarizes recent research progress on utilizing lignin and modified lignin for bio-based polyurethane synthesis with a focus on elastomers and foams. Opportunities and challenges for application of the lignin-based polyurethanes in various fields are also discussed.
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http://dx.doi.org/10.1002/marc.202000492DOI Listing
February 2021

Bone-targeting polymer vesicles for simultaneous imaging and effective malignant bone tumor treatment.

Biomaterials 2021 Feb 27;269:120345. Epub 2020 Aug 27.

Department of Orthopedics, Shanghai Institute of Bone Tumor, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China; Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, Shanghai, 201804, China. Electronic address:

We present a bone-targeting polymer vesicle with excellent single photon emission computed tomography/computed tomography (SPECT/CT) imaging capability and high antitumor drug delivery efficiency as an integrated platform for the simultaneous diagnosing and treatment of malignant bone tumors. This polymer vesicle can be self-assembled from poly(ε-caprolactone)-b-poly[(L-glutamic acid)-stat-(L-glutamic acid-alendronic acid)] (PCL-b-P[Glu-stat-(Glu-ADA)]), directly in water without the aid of a cosolvent. SPECT/CT dynamically tracked the drug distribution in the bone tumor rabbit models, and the tumor size was significantly reduced from >2.0 cm to <0.6 cm over 11 days. The pathological analysis demonstrated obvious necrosis and apoptosis of the tumor cells. Overall, this bone-targeting polymer vesicle provides us with a new platform for the combination of real-time diagnosis and therapy of malignant bone tumors.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120345DOI Listing
February 2021