Publications by authors named "Ning Xu"

923 Publications

Overexpression of serum extracellular vesicle microRNA-215-5p is associated with early tumor recurrence and poor prognosis of gastric cancer.

Clinics (Sao Paulo) 2021 5;76:e2081. Epub 2021 May 5.

Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.

Objectives: Extracellular vesicle microRNAs (EV-miRNAs) have been demonstrated to be reliable candidate biomarkers for clinical applications. However, the clinical application potential of serum EV-miR-215-5p for gastric cancer (GC) remains poorly understood. The goal of our study was to determine the efficacy of serum EV-miR-215-5p in predicting the prognosis of GC.

Methods: Blood samples were collected from 118 patients with GC, 60 patients with benign gastric disease and BGD and 70 healthy controls. The relative levels of serum EV-miR-215-5p were measured using quantitative real-time polymerase chain reaction (qRT-PCR).

Results: Compared to patients with BGD and normal controls, GC patients exhibited remarkably higher serum EV-miR-215-5p level, especially those with early tumor recurrence (ETR). Receiver operating characteristic (ROC) curve analysis showed that serum EV-miR-215-5p was able to distinguish GC patients from BGD patients or healthy controls and GC patients with ETR from those without ETR. In addition, increased serum EV-miR-215-5p levels were notably correlated with invasive depth, TNM stage, and lymph node metastasis. Moreover, serum EV-miR-215-5p levels were greatly decreased after surgical treatment, but increased at the time of ETR. Survival analysis showed that patients with higher serum EV-miR-215-5p had shorter survival. Furthermore, serum EV-miR-215-5p was an independent risk factor for GC.

Conclusions: Serum EV-miR-215-5p might be a novel biomarker for predicting ETR and prognosis of GC.
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http://dx.doi.org/10.6061/clinics/2021/e2081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075109PMC
May 2021

The role of positive nerve root sedimentation sign in the treatment of patients undergoing lumbar disc herniation.

Br J Neurosurg 2021 May 12:1-6. Epub 2021 May 12.

Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, PR China.

Background: To investigate the efficacy of percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of patients who were diagnosed with lumbar disc herniation (LDH) with positive nerve root sedimentation sign (NRSS).

Methods: A total of 86 patients who underwent LDH were recruited and divided into NRSS-positive group ( = 49) and NRSS-negative group ( = 37). The visual analog scale (VAS), Japanese Orthopaedic Association (JOA), and Oswestry disability index (ODI) were used to evaluate the low back pain and functional recovery and were compared between the two groups.

Results: There were no significant differences in the demographic parameters between the two groups. The average area of the dural sac compression in the NRSS-positive group was significantly higher than that in the NRSS-negative group. Patients with a positive NRSS showed a better low back pain relief than those with a negative NRSS at 1 week and 1 month after surgery. The JOA and ODI in the NRSS-positive group were better than those in the NRSS-negative group at 3 months and 6 months postoperatively. The apparent efficiency of JOA and the excellent and good rate of ODI in the negative group was lower than that in the positive group at 6 months after surgery.

Conclusions: Patients undergoing LDH with a positive NRSS showed better pain relief and functional recovery than those with a negative NRSS. The present study suggested that NRSS might be a valuable sign and associated with better clinical outcomes in patients undergoing LDH with the treatment of PTED.
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http://dx.doi.org/10.1080/02688697.2021.1923652DOI Listing
May 2021

Application of high-frequency oscillation ventilation combined with volume guarantee in infants with acute hypoxic respiratory failure after congenital heart surgery.

Pediatr Pulmonol 2021 May 8. Epub 2021 May 8.

Department of Cardiac Surgery, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Objective: This study aimed to evaluate the efficacy and safety of high-frequency oscillation ventilation combined with volume guarantee (HFOV-VG) compared with the safety and efficacy of HFOV alone in infants with acute hypoxemic respiratory failure (AHRF) after congenital heart surgery.

Methods: We retrospectively analyzed the clinical data of 44 infants who were ventilated for AHRF after congenital heart surgery between January 2020 and January 2021. HFOV alone was used in 23 of the 44 infants, whereas HFOV-VG was used in the other 21 infants.

Results: The average frequency tidal volume (VThf) of the HFOV-VG group was lower than that of the HFOV group, and the proportion of VThf exceeding the target range of infants in the HFOV-VG group was also lower (p < .01). In addition, the incidence of hypocapnia and hypercapnia in infants supported with HFOV-VG was significantly lower (p < .01). Furthermore, the duration of invasive ventilation and the median ventilator adjustment per hour in the HFOV-VG group was also lower than that in the HFOV group (p < .01).

Conclusions: Compared with HFOV alone, HFOV-VG decreases the fluctuation of VThf and the incidence of hypercapnia and hypocapnia. Moreover, it reduces the workload of bedside medical staff. Further studies are needed to confirm the efficacy and safety of HFOV-VG as a routine respiratory support strategy for congenital heart disease during the perioperative period.
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http://dx.doi.org/10.1002/ppul.25447DOI Listing
May 2021

Priming Leukemia with 5-Azacytidine Enhances CAR T Cell Therapy.

Immunotargets Ther 2021 28;10:123-140. Epub 2021 Apr 28.

Children's Cancer Institute, University of New South Wales, Sydney, NSW, Australia.

Purpose: Despite the success of chimeric antigen receptor (CAR) T cells in clinical studies, a significant proportion of responding patients eventually relapsed, with the latter correlating with low CAR T cell expansion and persistence.

Methods And Results: Using patient-derived xenograft (PDX) mouse models of CD19 B cell acute lymphoblastic leukemia (B-ALL), we show that priming leukemia-bearing mice with 5-azacytidine (AZA) enhances CAR T cell therapy. AZA given 1 day prior to CAR T cell infusion delayed leukemia growth and promoted CAR T cell expansion and effector function. Priming leukemia cells with AZA increased CAR T cell/target cell conjugation and target cell killing, promoted CAR T cell divisions and expanded IFNγ effector T cells in co-cultures with CD19 leukemia Nalm-6 and Raji cells. Transcriptome analysis revealed activation of diverse immune pathways in leukemia cells isolated from mice treated with AZA. We propose that epigenetic priming with AZA induces transcriptional changes that sensitize tumor cells to subsequent CAR T cell treatment. Among the candidate genes up-regulated by AZA is which encodes OX40L, one of the strongest T cell co-stimulatory ligands. OX40L binds OX40, the TNF receptor superfamily member highly specific for activated T cells. is heterogeneously expressed in a panel of pediatric PDXs, and high expression correlated with increased CAR T cell numbers identified in co-cultures with individual PDXs. High OX40L expression in Nalm-6 cells increased their susceptibility to CAR T cell killing while OX40L blockade reduced leukemia cell killing.

Conclusion: We propose that treatment with AZA activates OX40L/OX40 co-stimulatory signaling in CAR T cells. Our data suggest that the clinical use of AZA before CAR T cells could be considered.
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http://dx.doi.org/10.2147/ITT.S296161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091475PMC
April 2021

Red-Light-Responsive Ru Complex Photosensitizer for Lysosome Localization Photodynamic Therapy.

ACS Appl Mater Interfaces 2021 May 26;13(17):19572-19580. Epub 2021 Apr 26.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.

Photoresponsive ruthenium (Ru) complexes have been extensively studied in the photodynamic therapy (PDT) of cancer. The metal-to-ligand charge transfer (MLCT) absorption maximum of most Ru complexes is located in the short-wavelength visible region, which is well suited for superficial tumors but shows inefficient therapeutic effects for more deep-seated ones. Moreover, Ru complexes are primarily located in the mitochondria or nucleus, always resulting in high levels of dark toxicity and DNA mutation. Herein, we reported a new ruthenium complex (Ru-I) for red-light-triggered PDT. The activation wavelength of Ru-I is successfully extended to 660 nm. Importantly, the complex photosensitizer can be quickly taken up by cancer cells and selectively accumulated in the lysosome, an ideal localization for PDT purposes. Intratumoral injection of Ru-I into tumor-bearing mice achieved excellent therapeutic effects and thus holds great promise for applications in lysosome localization photodynamic therapy.
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http://dx.doi.org/10.1021/acsami.0c22551DOI Listing
May 2021

Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats.

Environ Sci Pollut Res Int 2021 Apr 25. Epub 2021 Apr 25.

College of Basic Medicine & Xu Rongxiang Regenerative Medicine Research Center, Binzhou Medical University, Yantai, People's Republic of China.

As a persistent pollutant, microplastics (MPs) have been reported to induce sperm quantity decrease in mice. However, the related mechanism remains obscure. Therefore, this study is intended to explore the effects of polystyrene microplastics (PS-MPs) on male reproduction and its related mechanism of blood-testis barrier (BTB) impairment. Thirty-two adult male Wistar rats were divided randomly into four groups fed with PS-MPs for 90 days at doses of 0 mg/day (control group), 0.015 mg/day, 0.15 mg/day, and 1.5 mg/day, respectively. The present results have shown that PS-MP exposure led to the damage of seminiferous tubule, resulted in apoptosis of spermatogenic cells, and decreased the motility and concentration of sperm, while the abnormality of sperm was elevated. Meanwhile, PS-MPs could induce oxidative stress and activate the p38 MAPK pathway and thus deplete the nuclear factor erythroid-2 related factor 2 (Nrf2). Noteworthily, PS-MPs led to the BTB-related protein expression decrease. All these results demonstrated that PS-MP exposure may lead to the destruction of BTB integrity and the apoptosis of spermatogenic cells through the activation of the MAPK-Nrf2 pathway. The current study provided novelty evidence for elucidating the effects of PS-MPs on male reproductive toxicity and its potential mechanism.
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http://dx.doi.org/10.1007/s11356-021-13911-9DOI Listing
April 2021

Reply to: "Myofibroblast YAP/TAZ is dispensable for liver fibrosis in mice".

J Hepatol 2021 Apr 20. Epub 2021 Apr 20.

Dumont-UCLA Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, David Geffen School of Medicine at University of California-Los Angeles, Los Angeles, CA, USA. Electronic address:

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http://dx.doi.org/10.1016/j.jhep.2021.04.014DOI Listing
April 2021

Antiparasitic Efficacy of Herbal Extracts and Active Compound Against in .

Front Vet Sci 2021 6;8:665072. Epub 2021 Apr 6.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

spp. Nordmann, 1832 (Monogenea: Gyrodactylidae) are common ectoparasites of teleost fishes. Infection with these parasites can increase the mortality of fish and cause considerable economic losses in intensive aquaculture. To find an effective antiparasitic agent for the control of gyrodactylosis, antiparasitic efficacy of crude extracts of 36 herbal medicines was evaluated using a (Cypriniformes, Cyprinidae)- model. Among all tested medicines, methanol extract of var. (Dioscoreales, Dioscoreaceae) was the most efficient, with an EC value of 4.17 mg/L. This extract showed 100% antiparasitic efficacy against at 10 mg/L and had a therapeutic index (TI, LC/EC) of 5.26, which is higher than that of formaldehyde (TI = 4.58), a widely used parasiticide in aquaculture. Subsequently, the potential mechanism of antiparasitic activity of dioscin, an active compound isolated from var. was investigated and the histopathological alterations in goldfish after exposure to dioscin were also studied. The trial indicated dioscin showed significant antiparasitic activity with a 24 h-EC value of 1.58 mg/L and it exhibited 100% antiparasitic efficacy at 0.6 mg/L. Also, could be completely removed within 2 h at 0.6 mg/L dioscin. Whereas, mean survival time of this worm was 4.99 h, and some individuals even reached 12 h at the same concentration of dioscin. These results indicated that 0.6 mg/L of dioscin did not completely kill all worms within 2 h, but just temporarily remove the worms from goldfish. Scanning electron microscopy (SEM) analysis showed that most of the microvilli on the tegument surface of dropped after exposure to dioscin. This might be one of the potential mechanisms of antiparasitic activity of dioscin against . Furthermore, no severe histopathological alteration was observed after exposure to a high concentration of dioscin for a short time. Considering both effectiveness and safety, therapeutic baths with a high concentration of dioscin for a short time might be a more optimal choice for the treatment of gyrodactylosis in aquaculture.
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http://dx.doi.org/10.3389/fvets.2021.665072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056006PMC
April 2021

Stability and L-gain analysis of nonlinear positive Markov jump systems based on a switching transition probability.

ISA Trans 2021 Apr 12. Epub 2021 Apr 12.

School of Control Science and Engineering, Dalian University of Technology, Dalian 116024, PR China. Electronic address:

This paper focuses on mean exponential stability and L-gain analysis for nonlinear positive Markov jump systems (NPMJSs) based on a switching transition probability (STP), where sector nonlinear functions and delays are adopted. By developing a nonlinear stochastic copositive Lyapunov-Krasovskii functional (NSCLKF) approach, a sufficient condition for mean exponential stability of nonlinear positive time-delay Markov jump system is first presented under mode-dependent average dwell time (MDADT) switching by using linear programming (LP) approach. Further, L-gain performance analysis is obtained. Moreover, the corresponding results for NPMJSs are given under average dwell time (ADT) switching. Illustrative results are provided to verify the validity of the theoretical results.
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http://dx.doi.org/10.1016/j.isatra.2021.04.007DOI Listing
April 2021

Review of Approaches to Developing Intersegmental Plane during Segmentectomy.

Thorac Cardiovasc Surg 2021 Apr 16. Epub 2021 Apr 16.

Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

In recent years, with the popularity of computed tomography (CT) scanning, early lung cancer has been found in a large number of patients, and segmentectomy has been widely used in clinical practice. The development of intersegmental plane is the most critical step in segmentectomy. At present, there are many methods to identify the intersegmental plane. Also, dissection of the intersegmental plane has been a challenge for thoracic surgeons for decades because of the complicated anatomic variations. This study focuses on the safety and efficacy of relevant methods in both identification and dissection of the intersegmental plane in segmentectomy.
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http://dx.doi.org/10.1055/s-0041-1727171DOI Listing
April 2021

Apolipoprotein M promotes cholesterol uptake and efflux from mouse macrophages.

FEBS Open Bio 2021 Apr 8. Epub 2021 Apr 8.

Clinical Medical Research Center, the Third Affiliated Hospital of Soochow University, Changzhou, China.

Apolipoprotein M (ApoM) exhibits various anti-atherosclerotic functions as a component of high-density lipoprotein (HDL) particles. Scavenger receptor class B type I (SR-BI) is a classic HDL receptor that mediates selective cholesterol uptake and enhances the efflux of cellular cholesterol to HDL. However, the effect of ApoM on cholesterol transport in macrophages remains unclear. In this study, we identified for the first time that ApoM is expressed in mouse macrophages and is involved in cholesterol uptake, similar to SR-BI. NBD-cholesterol uptake and efflux in cells were characterized using fluorescence spectrophotometry. The uptake ratios of cholesterol by macrophages from ApoM SR-BI mice were significantly lower than those from ApoM SR-BI and ApoM SR-BI mice. Real-time fluorescence quantitative PCR was used to analyze the expression of cholesterol transport-related genes involved in cholesterol uptake. ApoM-enriched HDL (ApoM HDL) facilitated more cholesterol efflux from murine macrophage Ana-1 cells than ApoM-free HDL (ApoM HDL). However, recombinant human ApoM protein inhibited the ability of ApoM HDL to induce cholesterol efflux. In conclusion, ApoM promotes cholesterol uptake and efflux in mouse macrophages. A better understanding of ApoM function may lead to the development of novel therapeutic strategies for treating atherosclerotic diseases.
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http://dx.doi.org/10.1002/2211-5463.13157DOI Listing
April 2021

Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring of Infection.

Int J Nanomedicine 2021 29;16:2477-2486. Epub 2021 Mar 29.

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, People's Republic of China.

Purpose: Sensitive and selective point-of-care biosensor is an urgent pursuit of serological antibody detection to control parasite pathogen. For specific, quantitative and on-site screening of infection in livestock, a quantum dot nanobead-monoclonal antibody (QB-mAb) probe-based immunochromatographic assay (ICA) was developed by introducing a competitive sandwich strategy (QB-CICA).

Methods: In the QB-CICA, QB-mAb probes competed with serum antibody for a particular epitope, followed by immunocomplexes binding to capture antibody on the test line. With the accumulation of target antibody, captured probes served as signal elements for fluorescent readout in a "turn off" mode, along with the fluorescence gradually weakened. The sensitivity and standard calibration curve of the QB-CICA were quantified using swine sera as negative control (n = 200) and artificial infected swine sera (n = 80) compared with a commercial ELISA kit. Besides, -antibody targeting test ability of the QB-CICA, instead of other parasites or viruses antibodies (n = 10), was evaluated.

Results: The QB-CICA exhibited a good linear range, a low detection limit of 189.92 ng mL and 100% selectivity that was higher than commercial ELISA kit (90%), as well as the same serological positive rate (100%) with commercial ELISA kit in different infection dose models.

Conclusion: Taking advantage of its simplicity, short response time (25 min), sensitivity and specificity, the proposed QB-CICA has potential applications for parasite-related antibody monitoring in food safety and clinical diagnosis fields.
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http://dx.doi.org/10.2147/IJN.S304845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018372PMC
April 2021

Multifunctional metalens generation using bilayer all-dielectric metasurfaces.

Opt Express 2021 Mar;29(6):9332-9345

Optical metasurfaces exhibit unprecedented ability in light field control due to their ability to locally change the phase, amplitude, and polarization of transmitted or reflected light. We propose a multifunctional metalens with dual working modes based on bilayer geometric phase elements consisting of low-loss phase change materials (SbSe) and amorphous silicon (a-Si). In transmission mode, by changing the crystalline state of the SbSe scatterer, a bifocal metalens with an arbitrary intensity ratio at the telecommunication C-band is realized, and the total focusing efficiency of the bifocal metalens is as high as 78%. Also, at the resonance wavelength of the amorphous SbSe scatterer, the scatterer can be regarded as a half-wave plate in reflection mode. The multifunctional metalens can reversely converge incident light into a focal point with a focusing efficiency of up to 30%. The high focusing efficiency, dynamic reconfigurability, and dual working modes of the multifunctional metalens contribute to polarization state detection, optical imaging, and optical data storage. In addition, the bilayer geometric phase elements can be easily extended to multilayer, which significantly improves the capability of manipulating the incident light field.
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http://dx.doi.org/10.1364/OE.420003DOI Listing
March 2021

Determination of Pharmacokinetic and Pharmacokinetic-Pharmacodynamic Parameters of Doxycycline against in Yellow Catfish ().

Antibiotics (Basel) 2021 Mar 21;10(3). Epub 2021 Mar 21.

Institute of Computational Comparative Medicine (ICCM), Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.

This study aimed to examine the pharmacokinetics of doxycycline (DC) in yellow catfish () and to calculate related pharmacokinetic-pharmacodynamic (PK/PD) parameters of DC against . The minimum inhibitory concentration of DC against was determined to be 500 µg/L. As the increase of oral dose from 10 to 40 mg/kg, the area under the concentration vs. time curve from 0 to 96 h (AUC) values were considerably increased in gill, kidney, muscle and skin, and plasma, except in liver. C values exhibited a similar dose-dependent increase trend in plasma and tissues except in liver, but other PK parameters had no apparent dose-dependence. The PK/PD parameter of the ratio of AUC to minimum inhibitory concentration (AUC/MIC) was markedly increased in plasma and tissues dose-dependently except in liver, but %T > MIC values were increased only moderately at some dose groups. After receiving the same dose with disparate time intervals from 96 to 12 h, the AUC/MIC was distinctly increased in plasma and tissues, but the %T > MIC had a decreasing trend. When administering 20 mg/kg with a time interval of 96 h, the AUC/MIC values were consistently >173.03 h and the %T > MIC values were above 99.47% in plasma and all tissues. These results suggest that administration of DC at 20 mg/kg every 96 h is a preferable regimen in yellow catfish.
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http://dx.doi.org/10.3390/antibiotics10030329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004065PMC
March 2021

Electrically-Driven Zoom Metalens Based on Dynamically Controlling the Phase of Barium Titanate (BTO) Column Antennas.

Nanomaterials (Basel) 2021 Mar 14;11(3). Epub 2021 Mar 14.

Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China.

The zoom metalens has been a research hotspot for metasurfaces in recent years. There are currently a variety of zoom methods, including dual metalenses, micro-electromechanical system metalenses, polydimethylsiloxane metalenses and Alvarez metalenses. However, for most metalenses, zooming is achieved by manipulating the relative displacement of two or more metasurfaces. Therefore, these methods seem inadequate when faced with more precise zooming requirements, and the precise control of the phase distribution cannot be achieved. In this paper, we innovatively propose an electrically-driven zoom metalens (EZM) of one-dimensional based on dynamically controlling barium titanate (BaTiO, BTO) antennas. Using the electro-optic effect of BTO crystals, we can apply a voltage to change the refractive index of BTO nanopillars ( = 2.4-3.6), thereby accurately controlling the phase distribution of column antennas. The proposed EZM can achieve 5× zoom ( = 10-50 μm), with advantages, such as high-speed optical amplitude modulation, ultra-compactness, flexibility and replicability. It can be applied in fields that require ultra-compact beam focusing, zoom imaging, and microscopic measuring.
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http://dx.doi.org/10.3390/nano11030729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002013PMC
March 2021

Identification of vital prognostic genes related to tumor microenvironment in pheochromocytoma and paraganglioma based on weighted gene co-expression network analysis.

Aging (Albany NY) 2021 Mar 26;13(7):9976-9990. Epub 2021 Mar 26.

Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.

Pheochromocytoma and paraganglioma (PCPG) is a rare neuroendocrine tumor. This study aims to identify vital prognostic genes which were associated with PCPG tumor microenvironment (TME). We downloaded transcriptome data of PCPG from TCGA database and calculated the immune scores and stromal scores by using the ESTIMATE algorithm. DEGs related to TMB were then identified. We conducted WGCNA to further extract the TME-related modules. GO, KEGG pathway analysis, and PPI network were performed. Survival analysis was conducted to identify the hub genes associated with the prognosis of PCPG. A total of 150 PCPG samples were included in this study. We obtained 1507 and 2067 DEGs based on immune scores and stromal scores, respectively. WGCNA analysis identified the red module and brown module were correlated with immune sores while the turquoise module and red module were significantly associated with stromal scores. Functional enrichments analysis revealed that 307 TME-related genes were correlated with the inflammation or immune response. Survival analysis showed that three TME-relate genes (ADGRE1, CCL18, and LILRA6) were associated with PCPG prognosis. These three hub genes including ADGRE1, CCL18, and LILRA6 might be involved in the progression of PCPG and could serve as potential biomarkers and novel therapeutic targets.
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http://dx.doi.org/10.18632/aging.202754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064173PMC
March 2021

Accurate diagnosis of pulmonary nodules using a non-invasive DNA methylation test.

J Clin Invest 2021 Apr 1. Epub 2021 Apr 1.

Department of Pathology, School of Basic Medical Science, Southern Medical University, Guangzhou, China.

Background: Current clinical management of patients with pulmonary nodules involves either repeated LDCT/CT scans or invasive procedures yet causes significant patient misclassification. An accurate non-invasive test is needed to identify malignant nodules and reduce unnecessary invasive tests.

Method: We developed a diagnostic model based on targeted DNA methylation sequencing of 389 pulmonary nodule patients' plasma samples, and then validated in 140 plasma samples independently. We tested the model in different stages and subtypes of pulmonary nodules.

Results: A 100-feature model was developed and validated for pulmonary nodule diagnosis: the model achieved a ROC-AUC of 0.843 on 140 independent validation samples with an accuracy of 0.800. The performance was well maintained in, 1) 6-20 mm size subgroup (N=100), with a sensitivity of 1.000 and adjusted NPV of 1.000 at 10% prevalence; 2) stage I malignancy (N=90), with a sensitivity of 0.971; 3) different nodule types - solid nodules (N=78) with a sensitivity of 1.000 and adjusted NPV of 1.000, part-solid nodules (N=75) with a sensitivity of 0.947 and adjusted NPV of 0.983, and ground-glass nodules (N=67) with a sensitivity of 0.964 and adjusted NPV of 0.989 at 10% prevalence. This methylation test, called PulmoSeek, outperformed PET-CT and two clinical prediction models (Mayo and Veterans Affairs) in discriminating malignant pulmonary nodules from benign ones.

Conclusion: This study suggests that the blood-based DNA methylation model may provide a better test for classifying pulmonary nodules, which could help facilitate the accurate diagnosis of early-stage lung cancer from pulmonary nodule patients and guide clinical decisions.

Funding: The National Key Research and Development Program of China; Science and Technology Planning Project of Guangdong Province; The National Natural Science Foundation of China National.
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http://dx.doi.org/10.1172/JCI145973DOI Listing
April 2021

Kinesin family member 18B regulates the proliferation and invasion of human prostate cancer cells.

Cell Death Dis 2021 Mar 22;12(4):302. Epub 2021 Mar 22.

Department of Urology, Urology Research Institute, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China.

Expression of kinesin family member 18B (KIF18B), an ATPase with key roles in cell division, is deregulated in many cancers, but its involvement in prostate cancer (PCa) is unclear. Here, we investigated the expression and function of KIF18B in human PCa specimens and cell lines using bioinformatics analyses, immunohistochemical and immunofluorescence microscopy, and RT-qPCR and western blot analyses. KIF18B was overexpressed in PCa specimens compared with paracancerous tissues and was associated with poorer disease-free survival. In vitro, KIF18B knockdown in PCa cell lines promoted cell proliferation, migration, and invasion, and inhibited cell apoptosis, while KIF18B overexpression had the opposite effects. In a mouse xenograft model, KIF18B overexpression accelerated and promoted the growth of PCa tumors. Bioinformatics analysis of control and KIF18B-overexpressing PCa cells showed that genes involved in the PI3K-AKT-mTOR signaling pathway were significantly enriched among the differentially expressed genes. Consistent with this observation, we found that KIF18B overexpression activates the PI3K-AKT-mTOR signaling pathway in PCa cells both in vitro and in vivo. Collectively, our results suggest that KIF18B plays a crucial role in PCa via activation of the PI3K-AKT-mTOR signaling pathway, and raise the possibility that KIF18B could have utility as a novel biomarker for PCa.
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http://dx.doi.org/10.1038/s41419-021-03582-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985494PMC
March 2021

Bacterial effector targeting of a plant iron sensor facilitates iron acquisition and pathogen colonization.

Plant Cell 2021 Mar 5. Epub 2021 Mar 5.

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Acquisition of nutrients from different species is necessary for pathogen colonization. Iron is an essential mineral nutrient for nearly all organisms, but little is known about how pathogens manipulate plant hosts to acquire iron. Here we report that AvrRps4, an effector protein delivered by Pseudomonas syringae bacteria to plants, interacts with and targets the plant iron sensor protein BRUTUS (BTS) to facilitate iron uptake and pathogen proliferation in Arabidopsis thaliana. Infection of rps4 and eds1 by P. syringae pv. tomato (Pst) DC3000 expressing AvrRps4 resulted in iron accumulation, especially in the plant apoplast. AvrRps4 alleviates BTS-mediated degradation of bHLH115 and ILR3(IAA-Leucine resistant 3), two iron regulatory proteins. In addition, BTS is important for accumulating immune proteins EDS1 at both the transcriptional and protein levels upon Pst (avrRps4) infections. Our findings suggest that AvrRps4 targets BTS to facilitate iron accumulation and BTS contributes to RPS4/EDS1-mediated immune responses.
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http://dx.doi.org/10.1093/plcell/koab075DOI Listing
March 2021

Retrospective study on the effectiveness of a prevention strategy in a dental hospital during the COVID-19 pandemic.

Clin Oral Investig 2021 Mar 18. Epub 2021 Mar 18.

Department of Prosthodontics, The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang, 310006, Hangzhou, China.

Objectives: To evaluate the effectiveness of a prevention strategy against the spread of SARS-CoV-2 infection among dental hospital staff over a 3-month period.

Materials And Methods: The effectiveness of the prevention strategy, which adopted healthcare staff protective measures, including patient triage and correct usage of personal preventive equipment, was evaluated by SARS-CoV-2 detection and serological testing. Patients who visited the Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China, between January 31 and March 1 (lockdown period) and March 2 and April 27 (reopening period) in 2020 and in the same period in 2019 were included in the study. Patients' diagnosis, age, gender, and several undergoing aerosol-generating procedures during the study period were collected from the hospital's database. Corresponding data were compared year-on-year (2020-on-2019). A total of 757 hospital staff underwent SARS-CoV-2 detection and serological testing on April 28-29, 2020.

Results: During the lockdown and reopening period in 2020, the number of hospital visits was dramatically decreased to about 6% and 58%, respectively, compared with the same period in 2019. However, emergency visits were increased 16- and 6.4-fold. A total of at least 6654 patients (13.58%) underwent aerosol-generating procedures during the study period in the year 2020. All hospital staff were negative according to SARS-CoV-2 detection and serological testing (IgG, IgM) data.

Conclusion: During the study period in 2020, the total number of hospital visits dramatically reduced but emergency visits significantly increased. The prevention strategy implemented successfully prevented SARS-CoV-2 infection spread among healthcare workers in a dental hospital.

Clinical Relevance: The prevention strategy indicated patient triage and how to adopt preventive measures for controlling SARS-CoV-2 spread among healthcare workers. These data can be used as a reference for other sectors suffering from the COVID-19 pandemic.
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http://dx.doi.org/10.1007/s00784-021-03886-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972332PMC
March 2021

Near-infrared thermally modulated varifocal metalens based on the phase change material SbS.

Opt Express 2021 Mar;29(5):7925-7934

Focus-tunable metalenses play an indispensable role in the development of integrated optical systems. In this paper, the phase change material SbS is used in a thermally modulated varifocal metalens based on PB-phase for the first time. SbS not only has a real part of refractive index shift between the amorphous and crystalline state but also has low losses in both amorphous and crystalline states in the near-infrared region. By switching SbS between the two states, a metalens doublet with a variable focal length is proposed. Moreover, the full width at half maximum of each focal point is close to the diffraction limit. And the focusing efficiency can be over 50% for the two focal points. Together with the advantage of precise thermal control, the proposed metalens has great potential in the application of multi-functional devices, biomedical science, communication and imaging.
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http://dx.doi.org/10.1364/OE.420014DOI Listing
March 2021

WITHDRAWN: Endothelial progenitor cells deliver exogenous miR-126 via exosomes to accelerate wound healing in diabetes mellitus.

J Diabetes Complications 2020 Dec 31:107844. Epub 2020 Dec 31.

Department of Orthopaedics, Fengxian Central Hospital Affiliated to Southern Medical University, China. Electronic address:

This article has been withdrawn at the request of the author(s) and editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
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http://dx.doi.org/10.1016/j.jdiacomp.2020.107844DOI Listing
December 2020

Correlation of Lgr5 expression with clinicopathological features of colorectal cancer and its diagnostic and prognostic values.

J BUON 2021 Jan-Feb;26(1):87-92

Hepatobiliary and Pancreatic Surgery, the second affiliated Hospital of Kunming Medical University, Kunming 650000, P.R. China.

Purpose: To investigate the relationship between Leucine-rich repeat G-protein coupled receptor 5 (Lgr5) expression and clinicopathological features of colorectal cancer and its diagnostic and prognostic values.

Methods: The cancer and adjacent tissues of 98 patients with colorectal cancer undergoing resection in Jiangsu Cancer Hospital from 2011 to 2013 were enrolled. The intestinal mucosal tissue of 50 healthy subjects was enrolled as a control group. Western blot was used to detect the expression level of Lgr5 in colorectal cancer, Kaplan-Meier method to analyze the correlation of Lgr5 expression with the 5-year survival rate, Cox regression to analyze prognostic risk factors for the patients, and receiver operating characteristics (ROC) curve to analyze the diagnostic value of Lgr5 of the disease.

Results: The expression level of Lgr5 in the intestinal mucosal and adjacent tissues was significantly lower than that in the cancer tissue (p<0.05). The 5-year survival in the Lgr5 low expression group was higher than that in the Lgr5 high expression group (p=0.002). Lgr5 was an independent risk factor for the prognosis of colorectal cancer. The sensitivity, specificity and the area under the curve (AUC) of Lgr5 were 90.00%, 79.59% and 0.880, respectively (p<0.001).

Conclusion: Patients with a low 5-year survival rate have a high expression of Lgr5, which is closely related to the clinical staging, differentiation, depth of infiltration, lymph node metastasis, vascular invasion, liver metastasis and distant metastasis. Lgr5 is an independent prognostic risk factor for the patients and a better indicator for the diagnosis of colorectal cancer.
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March 2021

Effects of different oral care strategies on postoperative pneumonia in infants with mechanical ventilation after cardiac surgery: a prospective randomized controlled study.

Transl Pediatr 2021 Feb;10(2):359-365

Department of Cardiac Surgery, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Background: To explore the effects of different oral care strategies on postoperative pneumonia in infants with mechanical ventilation after cardiac surgery.

Methods: A prospective randomized controlled study was conducted at a hospital in Fujian Province, China. Participants were randomly divided into the breast milk oral care group, physiological saline oral care group, and sodium bicarbonate oral care group to explore the effects of different oral care strategies on postoperative pneumonia in infants on mechanical ventilation cardiac surgery.

Results: The mechanical ventilation duration, the hospitalization costs, and the length of intensive care unit (ICU) stay and postoperative hospital stay in the breast milk oral care group were significantly shorter than those in the physiological saline oral care group and the sodium bicarbonate oral care group. The incidence of postoperative pneumonia in the breast milk oral care group was 3.2%, which was significantly lower than that in the physiological saline oral care group (22.6%) and the sodium bicarbonate oral care group (19.4%).

Conclusions: Using breast milk for oral care in infants after cardiac surgery has a lower incidence of postoperative pneumonia than traditional oral care strategies of physiological saline and sodium bicarbonate, and it is worthy of clinical application.
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http://dx.doi.org/10.21037/tp-20-295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944187PMC
February 2021

Pharmacokinetics, bioavailability, and tissue disposal profiles of Tiamulin fumarate in Nile tilapia (Oreochromis niloticus) following oral and intravenous administrations.

J Vet Pharmacol Ther 2021 Mar 6. Epub 2021 Mar 6.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

Tiamulin fumarate (TIF) is a pleuromutilin antibiotic and has high activity against animal bacterial pathogens including aquatic bacterial pathogens. However, its pharmacokinetic profiles, tissue distribution characteristics and bioavailability in aquatic animals remain unknown. The objective of this study was to investigate the pharmacokinetics and tissue distribution regularities of TIF in tilapia (Oreochromis niloticus) following a single oral (PO) dose of 20 mg/kg body weight (bw) and a single intravenous (IV) dose of 5 mg/kg bw at 22 ± 1°C, respectively. TIF concentrations in tilapia plasma and tissues were determined using the isotope dilution HPLC-HESI-MS/MS procedure, which was validated according to the guidelines defined by US Food and Drug Administration. TIF was well distributed throughout the body compartments of tilapia judged by the apparent volume of distribution (V ) >1 L/kg (6.69 L/kg PO and 1.78 L/kg IV). TIF had a short mean residence time (MRT; 22.82 h PO and 14.61 h IV) and quick total body clearance (CL ) (0.62 L kg  h PO and 0.60 L kg  h IV). The total area under the curve (AUC ) of plasma were 32.25 μg h  ml (PO) and 8.30 μg h  ml (IV), respectively, and the oral absolute bioavailability (F%) of TIF was calculated to be approximately 97.1%. For tissue distribution, high concentrations of TIF were found in kidney, and the longest MRT was recorded in bile. The withdrawal time (WT) of TIF in muscle, skin, liver, kidney, gill, and bile was 3.75 (4) and 1.79 (2), 1.77 (2) and 2.06 (3), 6.41 (7) and 1.97 (2), 6.95 (7) and 3.98 (4), 4.92 (5) and 2.36 (3), and 7.06 (8) and 6.16 (7) days after PO and IV administration, respectively. The present investigations indicated that TIF was quickly absorbed, well distributed, rapidly eliminated in tilapia, and it could serve as reference data for establishing use regimen and provide useful information for the further development of TIF in aquaculture.
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http://dx.doi.org/10.1111/jvp.12957DOI Listing
March 2021

Midterm Prognosis of Sexagenary Patients after Transcatheter Device Closure of Atrial Septal Defects: a Single-Chinese Center Experience.

Braz J Cardiovasc Surg 2021 Mar 1. Epub 2021 Mar 1.

Department of Cardiac Surgery, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.

Introduction: The objective of this study is to evaluate the efficacy and midterm prognosis of transcatheter device closure of atrial septal defects (ASDs) in sexagenary patients in China.

Methods: Forty-six sexagenary patients who underwent transcatheter device closure of ASDs in our hospital were included in this study. The patients' preoperative and postoperative clinical symptoms, echocardiographic results, and quality of life were investigated and analyzed.

Results: Of the 46 sexagenary patients who participated in the study, 40 completed the study. After ASD closure, the clinical symptoms of the patients significantly improved, and the number of patients with dyspnea and palpitations significantly decreased after the operation. According to the echocardiographic results, few patients had a tiny residual shunt after closure, but the shunt disappeared completely at the three-month follow-up. The size of the right ventricular cavity was significantly smaller postoperatively compared with preoperatively. Regarding the patients' quality of life, their feedback in all dimensions of the 36-Item Short-Form Health Survey (or SF-36) was significantly improved at the three-month follow-up, and it remained improved at the one-year follow-up.

Conclusion: The clinical outcomes and subjective quality of life of sexagenary patients with ASDs improved significantly after transcatheter device closure of ASDs. Therefore, we believe that for sexagenary patients with ASDs, transcatheter device closure is a favorable treatment.
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http://dx.doi.org/10.21470/1678-9741-2020-0387DOI Listing
March 2021

WeChat-assisted pre-operative health education improves the quality of life of parents of children with ventricular septal defects: A prospective randomised controlled study.

J Paediatr Child Health 2021 May 3;57(5):664-669. Epub 2021 Mar 3.

Department of Cardiac Surgery, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Aim: This study aimed to explore the effect of implementing pre-operative WeChat-assisted health education for parents of children with restrictive ventricular septal defects to improve their quality of life.

Methods: A prospective randomised controlled study was conducted in a provincial hospital in China. Participants were randomly divided into the intervention group (WeChat group, n = 35) and the control group (leaflet group, n = 35). The quality of life of parents of children with restricted ventricular septal defects was studied.

Results: Compared with the leaflet group, the WeChat group had a significantly lower score for the pre-operative Self-Rating Anxiety Scale (SAS) (P < 0.05). The pre-operative SAS score in the WeChat group was significantly lower than that at the first visit (P < 0.05). However, the SAS score in the leaflet group was similar at the time of preoperation and the first visit (P > 0.05). The pre-operative scores of the World Health Organization Quality of Life-BREF (WHOQOL-BREF) scale in the physiological, psychological, social, and environmental fields and the total life quality score in the WeChat group were significantly higher than those of the leaflet group (P < 0.05).

Conclusion: Implementing pre-operative WeChat-assisted health education for parents of children with restrictive ventricular septal defects can effectively relieve their anxiety and improve their quality of life.
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http://dx.doi.org/10.1111/jpc.15307DOI Listing
May 2021

Impact of Music Therapy on Preoperative Anxiety and Degree of Cooperation With Anesthesia Induction in Children With Simple Congenital Heart Disease.

J Perianesth Nurs 2021 Feb 27. Epub 2021 Feb 27.

Department of Cardiac Surgery, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China; Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fujian Maternity and Child Health Hospital, Fuzhou, China; Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University, Fuzhou, China. Electronic address:

Purpose: To evaluate the impact of music therapy (MT) on preoperative anxiety and degree of cooperation with anesthesia induction in children with simple congenital heart disease (CHD).

Design: A randomized controlled clinical study.

Methods: Ninety children were randomly assigned to the MT and control groups. The MT groups underwent a preoperative 30-minute session of MT, whereas the control group did not listen to any music and had the same amount of quiet time. The modified Yale Preoperative Anxiety Scale-Short Form (mYPAS-SF) was used to evaluate the patients' preoperative anxiety on entering the anesthesia waiting area (T1), 10 minutes after entering the waiting area (T2), and during the initiation of anesthesia induction (T3); the degree of cooperation with anesthesia induction was assessed using the Induction Compliance Checklist. The mean arterial blood pressure (MAP) and heart rate (HR) at T1, T2, T3, and T4 (the time of successful anesthesia) were also recorded.

Findings: One child was excluded from the MT group, and one was excluded from the control group. No difference was found in the mYPAS-SF score, MAP, or HR between the two groups at T1. The mYPAS-SF scores of the MT group were significantly lower than those of the control group at T2 and T3. At T2, T3, and T4, the MAP and HR of the MT group were lower than those of the control group. A statistically significant difference was found in the Induction Compliance Checklist score between the two groups.

Conclusions: MT can reduce preoperative anxiety and improve the degree of cooperation with anesthesia induction.
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http://dx.doi.org/10.1016/j.jopan.2020.08.004DOI Listing
February 2021

Signatures of continuous hexatic-liquid transition in two-dimensional melting.

Soft Matter 2021 Mar 1;17(12):3397-3403. Epub 2021 Mar 1.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Microscale Magnetic Resonance and Department of Physics, University of Science and Technology of China, Hefei 230026, People's Republic of China.

Recent studies have shown that the melting of two-dimensional crystals can be either continuous or discontinuous, relying on multiple parameters such as particle stiffness, density, and particle size dispersity. However, what determines the continuity or discontinuity of the two-dimensional melting remains elusive. Here we study the two-dimensional melting of binary mixtures of soft-core particles. The two particle species are different in either particle size or particle stiffness. Starting with the mono-component systems which exhibit discontinuous hexatic-liquid transition, we gradually increase the particle size or stiffness dispersity and find that the hexatic-liquid coexistent region shrinks and eventually vanishes above a critical dispersity. Therefore, the growth of disorder caused by the particle size or stiffness dispersity leads to the discontinuous-continuous transition of the two-dimensional melting. We further find that as long as the melting is continuous the defect concentrations on the boundary between hexatic and liquid phases remain almost constant, accompanied by an almost constant correlation length. These characteristic defect concentrations and correlation length are universal and independent of particle interactions, temperature, and type of particle dispersity, which act as signatures of the continuous two-dimensional melting.
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http://dx.doi.org/10.1039/d0sm02199gDOI Listing
March 2021

Achieving the Super Gas-Wetting Alteration by Functionalized Nano-Silica for Improving Fluid Flowing Capacity in Gas Condensate Reservoirs.

ACS Appl Mater Interfaces 2021 Mar 26;13(9):10996-11006. Epub 2021 Feb 26.

College of Petroleum Engineering, China University of Petroleum (East China), Qingdao 266580, China.

It is well-known that the production of gas-condensate reservoirs is significantly affected by the liquid condensation near the wellbore region. Gas-wetting alteration can be one of the most effective approaches to alleviate condensate accumulation and improve liquid distribution. However, gas well deliverability is still limited because the wettability of cores is altered only from liquid-wetting to intermediate gas-wetting by using traditional chemical stimulation. To solve this bottleneck problem, herein, we developed a fluorine-functionalized nanosilica to achieve super gas-wetting alteration, increasing the contact angles of water and n-hexadecane on the treated core surface from 23 and 0° to 157 and 145°, respectively. The surface free energy reduces rapidly from 67.97 to 0.23 mN/m. The super gas-wetting adsorption layer on the core surface formed by functionalized nanosilica not only increases the surface roughness but also reduces the surface free energy. The core flooding confirms that the required pressure for displacement is apparently reduced. Meanwhile, the core permeability can be dramatically restored after the super gas-wetting alteration. The microscopic visualization is employed to further understand the impact of fluorine-functionalized nanosilica on the fluid flow behavior and mechanism in porous media. The oil saturation in the micromodel decreases sharply from 48.75 to 7.84%, eliminating the "water locking effect" and "Jiamin effect", which indicates that the added functional nanosilica effectively improves fluid flow capacity and may contribute to production in the gas condensate reservoirs. In addition, this work reveals the fluid flow behavior and mechanism in the reservoir in detail, which will expand the better application of this material to many oilfields and other mining engineering systems.
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http://dx.doi.org/10.1021/acsami.0c22831DOI Listing
March 2021