Publications by authors named "Ning Wang"

3,837 Publications

  • Page 1 of 1

HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma.

Cancer Med 2022 Aug 10. Epub 2022 Aug 10.

Respiratory Disease Key Laboratory of Qingdao, Qingdao Municipal Hospital, Qingdao, China.

Background: Heart development protein with EGF-like domains 1 (HEG1), generally related to angiogenesis and embryonic development, was reported to participate in the occurrence and progression of some tumors recently. However, the role of HEG1 in lung adenocarcinoma (LUAD) is unclear.

Patients And Methods: To explore the effect of HEG1 on LUAD, GEPIA platform and UALCAN database, as well as Kaplan-Meier plotter were adopted to analyze the association of HEG1 with clinicopathological characteristics and survival outcomes for LUAD firstly. And then the HEG1 in LUAD tissues, blood and cell lines were detected by qRT-PCR, western blot, immunofluorescence, immunohistochemistry, and ELISA. Gene set enrichment analysis (GSEA) was conducted to identify pathways that might be affected by HEG1 in LUAD.

Results: In this study, HEG1 in lung tissues and cell lines of LUAD were significantly downregulated compared to benign pulmonary disease tissues and alveolar epithelial cells (p < 0.05). Moreover, compared with other groups, patients with advanced tumor stage had lower HEG1 mRNA expression levels (p = 0.025), which were negatively correlated with Ki67 index in tumor tissues (r = -0.427, p = 0.033). On the other hand, the LUAD patients with lower HEG1 had shorter overall survival (OS) (HR = 0.51, 95% CI: 0.40-0.65, p < 0.001) according to Kaplan-Meier plotter. In addition, HEG1 in serum of LUAD patients was negatively associated with CEA (r = -0.636, p < 0.001). GSEA showed that HEG1 was enriched in various metabolic-related pathways, including glucose metabolism, lipid metabolism, and nucleotide metabolism signaling.

Conclusions: HEG1 was downregulated in LUAD patients and associated with poor prognosis, which indicating HEG1 may serve as a potential biomarker for diagnosis and prognosis of LUAD.
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http://dx.doi.org/10.1002/cam4.5081DOI Listing
August 2022

Multi-omics analysis for potential inflammation-related genes involved in tumour immune evasion via extended application of epigenetic data.

Open Biol 2022 Aug 10;12(8):210375. Epub 2022 Aug 10.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, People's Republic of China.

Accumulating evidence suggests that inflammation-related genes may play key roles in tumour immune evasion. Programmed cell death ligand 1 (PD-L1) is an important immune checkpoint involved in mediating anti-tumour immunity. We performed multi-omics analysis to explore key inflammation-related genes affecting the transcriptional regulation of PD-L1 expression. The open chromatin region of the PD-L1 promoter was mapped using the assay for transposase-accessible chromatin using sequencing (ATAC-seq) profiles. Correlation analysis of epigenetic data (ATAC-seq) and transcriptome data (RNA-seq) were performed to identify inflammation-related transcription factors (TFs) whose expression levels were correlated with the chromatin accessibility of the PD-L1 promoter. Chromatin immunoprecipitation sequencing (ChIP-seq) profiles were used to confirm the physical binding of the TF STAT2 and the predicted binding regions. We also confirmed the results of the bioinformatics analysis with cell experiments. We identified chr9 : 5449463-5449962 and chr9 : 5450250-5450749 as reproducible open chromatin regions in the PD-L1 promoter. Moreover, we observed a correlation between STAT2 expression and the accessibility of the aforementioned regions. Furthermore, we confirmed its physical binding through ChIP-seq profiles and demonstrated the regulation of PD-L1 by STAT2 overexpression . Multiple databases were also used for the validation of the results. Our study identified STAT2 as a direct upstream TF regulating PD-L1 expression. The interaction of STAT2 and PD-L1 might be associated with tumour immune evasion in cancers, suggesting the potential value for tumour treatment.
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http://dx.doi.org/10.1098/rsob.210375DOI Listing
August 2022

Deep-Blue Electroluminescence of Perovskites with Reduced Dimensionality Achieved by Manipulating Adsorption-Energy Differences.

Angew Chem Int Ed Engl 2022 Aug 9. Epub 2022 Aug 9.

Jilin University, College of physics, Qianjin Street, 130022, Changchun, CHINA.

The lagging development of deep-blue perovskite light-emitting diodes (PeLEDs) heavily impedes their practical applications in full-color display due to the absence of spectrally stable emitters and the mismatch of carrier injection capacity. Herein, we report highly efficient deep-blue PeLEDs through a new chemical strategy that addresses the dilemma for simultaneously constant electroluminescence (EL) spectra and high-purify phase in reduced-dimensional perovskites. The success lies in the control of adsorption-energy differences between phenylbutylamine (PBA) and ethylamine (EA) interacting with perovskites, which facilitates narrow n-value distribution. This approach leads to an increased exciton binding energy and enhanced surface potential, hence improving radiative recombination. As a result, an external quantum efficiency of 4.62% is achieved in PeLEDs with a stable EL peak at 457 nm, demonstrating the best reported result for deep-blue PeLEDs so far.
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http://dx.doi.org/10.1002/anie.202210322DOI Listing
August 2022

Epstein-Barr virus infection-associated cholangiocarcinoma: a report of one case and the review of literature.

Virol J 2022 Aug 9;19(1):133. Epub 2022 Aug 9.

Department of Radiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.

The clinical data of a patient with Epstein-barr virus (EBV) associated with cholangiocarcinoma was reported in this paper: a case of a 36-year-old female presented with abdominal pain and systemic skin yellowing combined with skin itching. Laboratory studies showed increase in alanine aminotransferase 242 U/L, aspartate aminotransferase 404 U/L, r-glutamyltransferase 1516 U/L, total bilirubin 308.2 µmol/L and CA199 (101.0 U/ml). AFP (4.5 ng/ml) was normal. CT revealed multiple space-occupying lesions in the liver. PET-CT revealed liver malignant tumor and lymph node metastasis. Liver puncture pathology revealed infiltrative growth of significant heterocyst nests in the liver tissue, which was morphologically consistent with malignant tumors, considering poorly differentiated carcinoma. Pathology suggestion: combining liver puncture with morphology, immunohistochemistry, and EBV in situ hybridization results, it was consistent with EB virus-associated poorly differentiated carcinoma, therefore, consider EBV infection-associated poorly differentiated cholangiocarcinoma (CCA) (LELC morphology). The patient underwent liver transplantation in Hangzhou Shulan Hospital on June 8, 2021 successfully. After surgery, the patient orally took tacrolimus for anti-rejection, entecavir for antiviral therapy, gemcitabine 1.2 g + cis-platinum 30 mg for chemotherapy. After following up for more than 5 months post liver transplantation, the condition of the patient deteriorated. The patient subsequently died. Based on the case of our patient and the review of existing literature, when the patient's serum CA199 increased, AFP did not change significantly, and there was no previous history of hepatitis B. CT revealed a low-density mass in the liver, ring enhancement in the arterial phase, and heterogeneous enhancement of the tumor in the delayed phase. Ring enhancement of the liver lesion mass was observed on MRI. Consider the might possibility of hepatic CCA. When patients showed recurrent tonsillitis at an early age, EBV virus infection should be vigilant and oropharyngeal tissue should persist, diagnosis of EBV-associated liver cancer should be considered. In particular, EBV infection-related liver cancer is relatively rare, the clinician should improve the recognition of the disease to strive for early diagnosis and therapy.
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http://dx.doi.org/10.1186/s12985-022-01862-7DOI Listing
August 2022

Effect of lidocaine perioperative infusion on chronic postsurgical pain in patients undergoing thoracoscopic radical pneumonectomy.

BMC Anesthesiol 2022 Aug 9;22(1):255. Epub 2022 Aug 9.

Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou, 325027, China.

Background: Thoracoscopic radical pneumonectomy is associated with a high incidence of postoperative chronic pain. Studies on the benefits of lidocaine intravenous infusion during the perioperative period were still controversial in thoracoscopic surgery.

Methods: Sixty-four lung cancer patients scheduled for thoracoscopic radical pneumonectomy were randomly divided into two groups: normal saline group (control group) or lidocaine group. In the lidocaine group, 1.5 mg/kg lidocaine was administered during the anesthesia induction, and 2 mg·kg·h lidocaine was continuously intravenous infused until the end of the surgery. After the surgery, a mixture of 2 μg/kg sufentanil and 10 mg/kg lidocaine was continuously intravenous infused by postoperative patient-controlled intravenous analgesia pump (100 ml). In the control group, the same volume of normal saline was administered according to the calculation of lidocaine during anesthesia induction, maintenance and postoperative patient-controlled intravenous analgesia. The primary outcome was the incidence of chronic postoperative pain at 3 months after the surgery. The secondary outcomes include the incidence of chronic postoperative pain at 6 months after the surgery; the effect of lidocaine on postoperative pain within the first 24 and 48 h; total amount of sufentanil administered during entire procedure and the number of PCA triggers within 48 h after surgery.

Results: Compared with the control group, the incidence of chronic pain at 3 months after the surgery was significantly lower (13 cases, 46.4% vs. 6 cases, 20.7%, p < 0.05), but no significant difference at 6 months between two group. The cumulative dosage of sufentanil in perioperative period was significantly lower (149.64 ± 18.20 μg vs. 139.47 ± 16.75 μg) (p < 0.05), and the number of PCA triggers (8.21 ± 4.37 vs. 5.83 ± 4.12, p < 0.05) was significantly greater in the control group. The NRS pain scores at 24 h (1.68 ± 0.72 vs. 1.90 ± 0.86) and 48 h (1.21 ± 0.42 vs. 1.20 ± 0.41) after the operation were no significant difference.

Conclusion: Perioperative infusion lidocaine significantly reduced the number of PCA triggers and the incidence of chronic postoperative pain at 3 months after the thoracoscopic radical pneumonectomy.

Trial Registration: http://www.chictr.org.cn : ChiCTR1900024759, frist registration date 26/07/2019.
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http://dx.doi.org/10.1186/s12871-022-01795-2DOI Listing
August 2022

Incidence of Ischemia Reperfusion Injury Related Biliary Complications in Liver Transplantation: Effect of Different Types of Donors.

Transplant Proc 2022 Aug 4. Epub 2022 Aug 4.

Department of Organ Transplantation Center, The First Affiliated Hospital of University of Science and Technology of China, He Fei, China. Electronic address:

Liver transplantation from donors after circulatory death (DCD) is associated with considerable rates of primary nonfunction and ischemic-type biliary lesions. Compared with donation was after brain death (DBD), the biggest disadvantage of DCD is warm ischemia injury in the procurement stage. Donation after brain death followed by circulatory death (DBCD) is a unique practice in China. Such donors should donate according to the DCD procedure, that is, remove life support and donate after cardiac arrest. We retrospectively analyzed donor and recipient characteristics with preoperative and postoperative parameters according to 3 donation types to comprehensively describe incidence of ischemia reperfusion injury (IRI) related biliary complications among different donor type adult liver transplantation recipients. A total of 50 patients were included in this study (DBD group n = 17, DCD group n = 26, DBCD group n = 7). Only 1 patient, whose donor type was DBCD was diagnosed with ischemic-type biliary lesions demonstrated cast and retrograde ascending cholangitis. Rates of primary graft non-function (DBD n = 1, 5.9%; DCD n = 2, 7.7%; DBCD, 0%; P = .546) were similar and total biliary complications (DBD n = 1, 5.9%; DCD n = 1, 3.8%; DBCD N = 2, 28.6%; P = .042) were different. No differences were found regarding development of postreperfusion syndrome or coagulopathy in 3 groups. Compared with standard DBD donor, the clinical outcome of DCD donor liver transplantation was satisfactory, with no increase in the incidence of IRI, and, no difference in the incidence of ischemic bile duct complications. This work was carried out in compliance with the Helsinki Congress and the Declaration of Istanbul.
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http://dx.doi.org/10.1016/j.transproceed.2022.05.020DOI Listing
August 2022

New insight into the sucrose biosynthesis pathway from genome-wide identification, gene expression analysis, and subcellular localization in hexaploid wheat (Triticum aestivum L.).

J Plant Physiol 2022 Jul 16;276:153770. Epub 2022 Jul 16.

Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100048, China. Electronic address:

Sucrose, the main synthesized product and transported form of photoassimilates, moves from leaves to support plant growth and storage substance biosynthesis occurring in the heterotrophic sink organs. Enhancing sucrose biosynthesis efficiency is a top priority for crop yield breeding programs. However, the molecular mechanism of sucrose biosynthesis is still elusive, especially in wheat. We performed transcriptome sequencing, subcellular localization, and bioinformatics analysis to identify key isoforms and metabolic branches associated with sucrose biosynthesis in wheat. Our analysis identified 45 genes from 13 families that exhibited high expression in young leaves with an evident diurnal change. The carbon flux from photoassimilates to sucrose was divided into two pathways. In the cytoplasm, assimilates initiating at phosphotrioses (TPs) exported by TaTPT1 from chloroplasts flowed along the TP-Sucrose branch formed by TaALD6, TaFBP5, TacPGI, TacPGM, TaUGP1, TaSPS5, and TaSPP1. Intermediates either from the Calvin cycle or TP-Sucrose branch were converted into ADPGlc to synthesize the simple starch, which was transiently degraded by a series of enzymes, including TaBAM4 and TaSEX4 in the chloroplast. Similar to the starch-biosynthesis branch in endosperms, the TP-Sucrose branch is the most prominent in leaves because each reaction can be catalyzed by at least one highly expressed isoform with expected cytosolic localization. The key isoforms and major branches highlighted in the wheat sucrose biosynthesis pathway expand our molecular understanding of crop sucrose biosynthesis and provide clues to increase wheat yield by enhancing the sucrose synthesis efficiency of leaves.
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http://dx.doi.org/10.1016/j.jplph.2022.153770DOI Listing
July 2022

Tip-concentrated microneedle patch delivering everolimus for therapy of multiple sclerosis.

Biomater Adv 2022 Apr 22;135:212729. Epub 2022 Apr 22.

Wuya Collage of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address:

Multiple sclerosis is a chronic progressive demyelinating disease of the central nervous system. At present, systemic drug therapy for multiple sclerosis has limited efficacy and serious side effects. Everolimus, as a new generation of mTOR inhibitors, can effectively alleviate the inflammatory reaction of the central nervous system and offers a promising choice for the treatment of multiple sclerosis. However, due to the low oral bioavailability and narrow response window of oral everolimus, a new delivery system is urgently needed to overcome the above problems. In this study, we constructed a tip-concentrated microneedle patch as a transdermal delivery system of everolimus for the treatment of multiple sclerosis. Here, the drug was concentrated in the needle tips by the rational design, making it delivered completely into the skin. The therapeutic effect of everolimus-loaded microneedles was evaluated using the experimental autoimmune encephalomyelitis (EAE) model and further verified with neurological function scores and the histopathological results of the spinal cord. These results indicated that the tip-concentrated microneedle patch provided an effective, safe and simple method for the transdermal delivery of everolimus, thus providing a new treatment for multiple sclerosis.
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http://dx.doi.org/10.1016/j.bioadv.2022.212729DOI Listing
April 2022

Surgical treatment of giant right ventricular fibroma for a newborn: A case report.

Front Cardiovasc Med 2022 19;9:908287. Epub 2022 Jul 19.

Dalian Women and Children's Medical Center (Group), Dalian, China.

This report describes the surgical treatment of giant right ventricular fibroma in a newborn. Cardiac uhrasonography and CT showed a large mass in the right ventricle wall, which narrowed the right ventricular inflow tract. The newborn patient gradually developed symptoms such as shortness of breath, oliguria, and pericardial effusion. We performed tumor excision, but due to severe damage to the right ventricular wall and right heart failure, the patient relied on cardiopulmonary bypass. Then, we immediately restored the opening of the ductus arteriosus, enlarged the foramen ovale, and used various vasoactive drugs to ensure the smooth resuscitation of the patient. This is a kind of operation for the youngest patients. The perioperative treatment experience indicated the feasibility of excision of giant right ventricular fibroma for newborn patients.
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http://dx.doi.org/10.3389/fcvm.2022.908287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343619PMC
July 2022

The mitochondrial genome of Dokhtouroff, 1887 (Coleoptera: Meloidae).

Mitochondrial DNA B Resour 2022 14;7(6):1021-1023. Epub 2022 Jun 14.

Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China.

Dokhtouroff, 1887 is a traditional medicine material and an important predator in China. The mitochondrial genome of is presented for the first time in this study. The mitogenome is 15,034 bp in length and comprises 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and partial control region. The nucleotide composition of was 36.7% of A, 18.1% of C, 11.1% of G, and 34.1% of T. The phylogenetic results divide all Meloidae species into two clades. The genus was retrieved as a paraphyletic group, with having a closer relationship with than other genera within Meloidae. This study provides useful genetic data for future studies on the phylogeny and evolution of Meloidae species.
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http://dx.doi.org/10.1080/23802359.2022.2080601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344954PMC
June 2022

Superior Efficacy of a TBI-166, Bedaquiline, and Pyrazinamide Combination Regimen in a Murine Model of Tuberculosis.

Antimicrob Agents Chemother 2022 Aug 4:e0065822. Epub 2022 Aug 4.

Beijing Key Laboratory of Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.

TBI-166, derived from riminophenazine analogues, shows more potent anti-TB activity than clofazimine and is being assessed against tuberculosis (TB) in a phase IIa clinical trial in China. Preclinical regimen studies containing TBI-166 will support the phase IIb clinical trials of TBI-166. In the present study, we compared the efficacy in three murine TB models of an all-oral drug-resistant TB drug regimen of TBI-166 with bedaquiline (BDQ) and pyrazinamide (PZA) with the first-line regimen of isoniazid (INH) with rifampin (RFP) and PZA (HRZ regimen), the most effective reported TBI-166-containing regimen of TBI-166 with BDQ and linezolid (LZD), and the Nix-TB clinical trial regimen of BDQ with pretomanid and LZD (BPaL regimen). In the C3HeB/FeJ murine TB model, for the TBI-166+BDQ+PZA regimen, the lungs of mice were culture negative at 4 weeks, and there were no relapses at 8 weeks of treatment. The reduction in bacterial burden and relapse rate were greater than those of the HRZ regimen and the TBI-166+BDQ+LZD regimen. Compared with the BPaL regimen, the TBI-166+BDQ+PZA regimen had similar or stronger early bactericidal activity, bactericidal activity, and sterilizing activity in the BALB/c murine TB model. The bacterial burden in the TBI-166+BDQ+PZA regimen group decreased significantly more than that in the BPaL regimen group and was almost or totally relapse free (<13.33% after 8 weeks). In conclusion, oral short-course three-drug regimens, including TBI-166 with high efficacy, were identified. The TBI-166+BDQ+PZA regimen is recommended for further study in a TBI-166 phase IIb clinical trial.
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http://dx.doi.org/10.1128/aac.00658-22DOI Listing
August 2022

Uptake of lung cancer screening with low-dose computed tomography in China: A multi-centre population-based study.

EClinicalMedicine 2022 Oct 29;52:101594. Epub 2022 Jul 29.

Office of Cancer Screening, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Background: Optimal uptake rates of low-dose computed tomography (LDCT) scans are essential for lung cancer screening (LCS) to confer mortality benefits. We aimed to outline the process model of the LCS programme in China, identify the high-risk individuals with low uptake based on a prospective multi-centre population-based cohort, and further explore associated structural characteristics.

Methods: A total of 221,955 individuals at high-risk for lung cancer from the National Lung Cancer Screening cohort were included. The logistic regression model was performed to identify the individual characteristics associated with the uptake of LCS, defined as whether the high-risk individual undertook LDCT scans in designated hospitals within six months following the initial risk assessment. The linear regression model was adopted to explore the structural characteristics associated with the uptake rates in 186 communities.

Findings: The overall uptake rate was 33·0%. The uptake rate was negatively correlated with the incidence of advanced-stage lung cancer (Pearson's coefficient -0·88, 0·0007). Multivariable regression models found that lower uptake rates were associated with males (OR 0·88, 95%CI 0·85-0·91), current smokers (OR 0·93, 95%CI 0·90-0·96), individuals with depressive symptoms (OR 0·92, 95%CI 0·90-0·94), and the structural characteristics, including longer structural delays in initiating LDCT scans (30-90 days vs. ≤14 days: β -7·17, 95%CI -12·76∼ -1·57; >90 days vs. ≤14 days: β -13·69, 95%CI -24·61∼ -2·76), no media-assisted publicity (β -6·43, 95%CI -11·26∼ -1·60), and no navigation assistance (β -5·48, 95%CI -10·52∼ -0·44).

Interpretation: Multifaceted interventions are recommended, which focus on poor-uptake individuals and integrate the 'assessment-to-timely-screening' approach to minimise structural delays, media publicity, and a navigation assistance along the centralised screening pathway.

Funding: Ministry of Finance and National Health Commission of the People's Republic of China.
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http://dx.doi.org/10.1016/j.eclinm.2022.101594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340538PMC
October 2022

Tong-Qiao-Huo-Xue decoction activates PI3K/Akt/mTOR pathway to reduce BMECs autophagy after cerebral ischemia/reperfusion injury.

J Ethnopharmacol 2022 Jul 31:115585. Epub 2022 Jul 31.

Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Institute for Pharmacodynamics and Safety Evaluation of Chinese Medicine, Anhui Academy of Traditional Chinese Medicine, Hefei, 230012, PR China; College of Integrated Chinese and Western Medicine (College of Life Science), Anhui University of Chinese Medicine, Hefei, China.

Ethnopharmacological Relevance: Tong-Qiao-Huo-Xue Decoction (TQHXD) is a traditional classic Chinese Medicinal Formula (CMF) used for clinical treatment of ischemic stroke. TQHXD leads to improvement in the symptoms of the acute period of cerebral infarction and recovery period after stroke. Our previous studies also showed that TQHXD produced a significant protective effect on the brain after cerebral ischemia-reperfusion (I/R) injury. It is reported that autophagy is closely related to ischemic brain injury; however, the functional contribution of TQHXD to brain microvascular endothelial cell (BMEC) autophagy and its underlying mechanism remains unclear.

Aim Of The Study: The purpose of this study was to investigate the effects and mechanism of TQHXD in inhibiting cerebral ischemia-induced endothelial autophagy.

Materials And Methods: The high-performance liquid chromatography (HPLC) fingerprint of the chemical constituents from TQHXD was established for the quality control, and the Longa method was used to evaluate the efficacy of TQHXD in rats with middle cerebral artery occlusion (MCAO). The expression of LC3 was determined by immunofluorescence double staining. To evaluate the protective effects of TQHXD-containing cerebrospinal fluid (CSF) on BMECs injured by oxygen-glucose deprivation and reperfusion, cell survival rate was determined using the CCK-8 assay and cell apoptosis was determined by fluorescein isothiocyanate (FITC)-Annexin V/PI. Autophagy was detected using transmission electron microscopy.

Results: The results showed that TQHXD-CSF significantly ameliorated oxygen-glucose deprivation/reperfusion (OGD/R)-induced injury in BMECs. Confocal microscopy and western blot results showed that TQHXD-CSF reduced autophagy-related protein expression and autophagosome number. The results of the western blotting indicated that TQHXD-CSF caused a marked increase in the phosphorylation of protein kinase B and phosphoinsotide-3 kinase (Akt/p-Akt and PI3K/p-PI3K, respectively) and their expression levels were down-regulated after treatment with pathway inhibitor, ZSTK474. Furthermore, in a MCAO model in rats, TQHXD markedly increased p-PI3K, p-Akt and p-mTOR, whereas the autophagy related proteins decreased.

Conclusions: Taken together, these findings demonstrate that TQHXD protects against ischemic insult by inhibiting autophagy through the regulation of the PI3K/Akt/mammalian target of rapamycin (mTOR) pathway and that TQHXD may have therapeutic value for protecting BMECs from cerebral ischemia.
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http://dx.doi.org/10.1016/j.jep.2022.115585DOI Listing
July 2022

Association between sarcopenia and osteoarthritis: A protocol for meta-analysis.

PLoS One 2022 3;17(8):e0272284. Epub 2022 Aug 3.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Sarcopenia, a relatively new syndrome referring to the age-related decline of muscle strength and degenerative loss of skeletal muscle mass and function, often resulting in frailty, disability, and mortality. Osteoarthritis, as a prevalent joint degenerative disease, is affecting over 250 million patients worldwide, and it is the fifth leading cause of disability. Despite the high prevalence of osteoarthritis, there are still lack of efficient treatment potions in clinics, partially due to the heterogeneous and complexity of osteoarthritis pathology. Previous studies revealed the association between sarcopenia and osteoarthritis, but the conclusions remain controversial and the prevalence of sarcopenia within osteoarthritis patients still needs to be elucidated. To identify the current evidence on the prevalence of sarcopenia and its association with osteoarthritis across studies, we performed this systematic review and meta-analysis that would help us to further confirm the association between these two diseases.

Methods And Analysis: Electronic sources including PubMed, Embase, and Web of Science will be searched systematically following appropriate strategies to identify relevant studies from inception up to 28 February 2022 with no language restriction. Two investigators will evaluate the preselected studies independently for inclusion, data extraction and quality assessment using a standardized protocol. Meta-analysis will be performed to pool the estimated effect using studies assessing an association between sarcopenia and osteoarthritis. Subgroup analyses will also be performed when data are sufficient. Heterogeneity and publication bias of included studies will be investigated.

Prospero Registration Number: CRD42020155694.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0272284PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348705PMC
August 2022

Epidemiological Characteristics of Peripheral T-Cell Lymphoma: A Population-Based Study.

Front Oncol 2022 13;12:863269. Epub 2022 Jul 13.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Beijing Office for Cancer Prevention and Control, Peking University Cancer Hospital and Institute, Beijing, China.

Objects: The aim of this study is to explore the epidemiological characteristics of peripheral T-cell lymphoma in Beijing.

Methods:  All data were extracted from the Beijing Cancer Registry database from January 1, 2007, to December 31, 2018. Segi's World Standard Population was used to estimate the age-standardized rate (ASR). Changes in trends were examined using joinpoint regression analysis. The observed survival was estimated by the Kaplan-Meier method. Relative survival was calculated using Ederer II and standardized using the Brenner method and International Cancer Survival Standard (ICSS) group 1 age structure. Stratified by gender, area, and histological type, incidence, mortality, and age of onset trends were observed in Beijing.

Results:  In Beijing, there were 801 new cases and 463 deaths of T-cell lymphoma from 2007 to 2018. Peripheral T-cell lymphoma not otherwise specified (PTCL-NOS) was the most prevalent subtype (37.45%), followed by angioimmunoblastic T-cell lymphoma (AITL; 20.35%), NK/T-cell lymphoma (NK/TCL; 17.60%), and anaplastic large cell lymphoma (ALCL; 10.24%). The crude incidence and mortality rates were 0.52 and 0.30 per 100,000 person-years, respectively, whereas the age-standardized incidence and mortality rates (ASIR and ASMR) were 0.35 and 0.18 per 100,000 person-years, respectively. Both ASIR and ASMR were more prevalent in men (0.48 and 0.24 per 100,000) and urban area (0.38 and 0.19 per 100,000) than in women (0.22 and 0.11 per 100,000) and rural area (0.30 and 0.15 per 100,000). The average annual percentage change (AAPC) of ASIR and ASMR was 5.72% (95% confidence interval (CI): 1.79%-9.81%) and 4.35% (95% CI: -0.09%-8.99%), respectively. The age-specific incidence rate increased with age and peaked at the age groups of 10-14 and 80-84. The mean and median age of onset increased between 2007 and 2018. In addition, it decreased after the age of onset was age standardization (, ). The 5-year age-standardized relative survival was 39.02% for all patients, 58.14% for NK/TCL, 57.60% for ALCL, 31.38% for AITL, and 29.18% for PTCL-NOS.

Conclusions:  T-cell lymphoma incidence was rising, but survival was dismal in Beijing, indicating the need for improved early diagnosis and standardized treatment.
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http://dx.doi.org/10.3389/fonc.2022.863269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326059PMC
July 2022

Effective absorption of dichloromethane using deep eutectic solvents.

J Hazard Mater 2022 Jul 22;439:129666. Epub 2022 Jul 22.

Faculty of Environment and Life, Beijing University of Technology, 100 Ping Le Yuan, Chaoyang District, Beijing 100124, China. Electronic address:

Chlorinated volatile organic compounds (VOCs), of which dichloromethane (DCM) has become one of the main components because of its extensive use and strong volatility, are recognized as extremely hazardous and refractory pollutants in the atmosphere. The efficient treatment of DCM is of great significance to the protection of environment and human health. In this work, the strategy of DCM capture with deep eutectic solvents (DESs) with different hydrogen bond acceptors (HBAs) and hydrogen bond donors (HBDs) was proposed and systematically investigated. The experimental results show that tetrabutylphosphonium chloride: levulinic acid ([P][Cl]-LEV) presents the most excellent DCM absorption capacity among all DESs studied and considerable capacity in [P][Cl]-LEV (1:2) with 899 mg DCM/g DES (5.58 mol DCM/mol DES) at 30 °C and DCM partial pressure of 0.3 bar can be achieved. The microscopic absorption mechanism is explored by HNMR and FT-IR spectra as well as quantum chemistry calculations, indicating that the absorption is a physical process. The interaction energy analysis suggests that the greater the interaction energy between DES and DCM, the greater the saturated absorption capacity of DCM. The hydrogen bond (HB) contributes most to the weak interaction between DCM and HBA/HBD, and both HBA and HBD play an important role in the absorption of DCM.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129666DOI Listing
July 2022

Mechanistic Insights into OC-COH Coupling in CO Electroreduction on Fragmented Copper.

J Am Chem Soc 2022 Aug 29;144(31):14005-14011. Epub 2022 Jul 29.

School of Materials Science and Engineering and Key Laboratory of Efficient Utilization of Low and Medium Grade Energy, Tianjin University, Tianjin 300350, People's Republic of China.

The carbon-carbon (C-C) bond formation is essential for the electroconversion of CO into high-energy-density C products, and the precise coupling pathways remain controversial. Although recent computational investigations have proposed that the OC-COH coupling pathway is more favorable in specific reaction conditions than the well-known CO dimerization pathway, the experimental evidence is still lacking, partly due to the separated catalyst design and mechanistic/spectroscopic exploration. Here, we employ density functional theory calculations to show that on low-coordinated copper sites, the *CO bindings are strengthened, and the adsorbed *CO coupling with their hydrogenation species, *COH, receives precedence over CO dimerization. Experimentally, we construct a fragmented Cu catalyst with abundant low-coordinated sites, exhibiting a 77.8% Faradaic efficiency for C products at 300 mA cm. With a suite of spectroscopic studies, we capture an *OCCOH intermediate on the fragmented Cu surfaces, providing direct evidence to support the OC-COH coupling pathway. The mechanistic insights of this research elucidate how to design materials in favor of OC-COH coupling toward efficient C production from CO reduction.
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http://dx.doi.org/10.1021/jacs.2c01044DOI Listing
August 2022

Free Nitrous Acid Inhibits Atenolol Removal during the Sidestream Partial Nitritation Process through Regulating Microbial-Induced Metabolic Types.

Environ Sci Technol 2022 Jul 28. Epub 2022 Jul 28.

School of Civil and Environmental Engineering, Nanyang Technological University, 639798 Singapore.

Limited studies have attempted to evaluate pharmaceutical removal during the sidestream partial nitritation (PN) process. In this work, atenolol biodegradation by PN cultures was investigated by maintaining ammonium and pH at different levels. For the first time, free nitrous acid (FNA), other than ammonium, pH, and free ammonia, was demonstrated to inhibit atenolol removal, with biodegradation efficiencies of ∼98, ∼67, and ∼28% within 6 days at average FNA levels of 0, 0.03, and 0.19 mg-N L, respectively. Ammonia-oxidizing bacteria (AOB)-induced metabolism was predominant despite varying FNA concentrations. In the absence of ammonium/FNA, atenolol was mostly biodegraded via AOB-induced metabolism (65%) and heterotroph-induced metabolism (33%). AOB-induced metabolism was largely inhibited (down to 29%) at 0.03 mg-N L FNA, while ∼27 and ∼11% were degraded via heterotroph-induced metabolism and AOB-induced cometabolism, respectively. Higher FNA (0.19 mg-N L) substantially reduced atenolol biodegradation via heterotroph-induced metabolism (4%), AOB-induced metabolism (16%), and AOB-induced cometabolism (8%). Newly identified products and pathways were related to metabolic types and FNA levels: (i) deamination and decarbonylation (AOB-induced cometabolism, 0.03 mg-N L FNA); (ii) deamination from atenolol acid (heterotrophic biodegradation); and (iii) nitro-substitution (reaction with nitrite). This suggests limiting FNA to realize simultaneous nitrogen and pharmaceutical removal during the sidestream process.
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http://dx.doi.org/10.1021/acs.est.1c08845DOI Listing
July 2022

Enhances the Tolerance to Aluminum Stress in .

Int J Mol Sci 2022 Jul 25;23(15). Epub 2022 Jul 25.

Key Laboratory of Biology and Genetic Improvement of Maize in Southwest China of Agricultural Department, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China.

Aluminum (Al) toxicity causes severe reduction in crop yields in acidic soil. The natural resistance-associated macrophage proteins (NRAMPs) play an important role in the transport of mineral elements in plants. Recently, and were reported specifically to transport trivalent Al ions. In this study, we functionally characterized , a gene previously identified from RNA-Seq data from Al-treated maize roots, in response to Al exposure in maize. was predominantly expressed in root tips and was specifically induced by Al stress. Yeast cells expressing were hypersensitive to Al, which was associated with Al accumulation in yeast. Furthermore, overexpression of in conferred transgenic plants with a significant increase in Al tolerance. However, expression of , either in yeast or in , had no effect on the response to cadmium stress. Taken together, these results underlined an internal tolerance mechanism involving to enhance Al tolerance via cytoplasmic sequestration of Al in maize.
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http://dx.doi.org/10.3390/ijms23158162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331102PMC
July 2022

A Comparative Study on Optofluidic Fenton Microreactors Integrated with Fe-Based Materials for Water Treatment.

Micromachines (Basel) 2022 Jul 16;13(7). Epub 2022 Jul 16.

State Key Laboratory of Optical Fiber and Cable Manufacture Technology, Yangtze Optical Fiber and Cable Joint Stock Limited Company, Wuhan 430070, China.

The catalysts employed in catalytic reactors greatly affect the reaction efficiency of the reaction system and the reactor's performance. This work presents a rapid comparative study on three kinds of Fe-based materials integrated into an optofluidic Fenton reactor for water treatment. The Fe-based sheets (FeSiB, FeNbCuSiB, and FeNi) were respectively implanted into the reaction chamber to degrade the organic dyes with the assistance of HO. In the experiment, by adjusting the hydrogen peroxide concentration, flow rate, and light irradiation, the applicable conditions of the Fe-based materials for the dye degradation could be evaluated quickly to explore the optimal design of the Fenton reaction system. The results indicated that FeNi (1j85) exhibits excellent degradability in the microreactor, the reaction rate can reach 23.4%/s at the flow rate of 330 μL/min, but its weak corrosion resistance was definitely demonstrated. Although the initial degradability of the microreactor by using FeNbCuSiB (1k107) was not as good as that of 1j85, it increased after being reused several times instead, and the degradation efficiency reached >98% after being reused five times. However, the FeSiB (1k101) material shows the worst degradability and recycling. Therefore, in contrast, 1k107 has the greatest potential to be used in Fenton reactors for practical water treatment.
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http://dx.doi.org/10.3390/mi13071125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317202PMC
July 2022

Detecting Errors with Zero-Shot Learning.

Authors:
Xiaoyu Wu Ning Wang

Entropy (Basel) 2022 Jul 6;24(7). Epub 2022 Jul 6.

School of Computer and Information Technology, Beijing Jiaotong University, Beijing 100044, China.

Error detection is a critical step in data cleaning. Most traditional error detection methods are based on rules and external information with high cost, especially when dealing with large-scaled data. Recently, with the advances of deep learning, some researchers focus their attention on learning the semantic distribution of data for error detection; however, the low error rate in real datasets makes it hard to collect negative samples for training supervised deep learning models. Most of the existing deep-learning-based error detection algorithms solve the class imbalance problem by data augmentation. Due to the inadequate sampling of negative samples, the features learned by those methods may be biased. In this paper, we propose an AEGAN (Auto-Encoder Generative Adversarial Network)-based deep learning model named SAT-GAN (Self-Attention Generative Adversarial Network) to detect errors in relational datasets. Combining the self-attention mechanism with the pre-trained language model, our model can capture semantic features of the dataset, specifically the functional dependency between attributes, so that no rules or constraints are needed for SAT-GAN to identify inconsistent data. For the lack of negative samples, we propose to train our model via zero-shot learning. As a clean-data tailored model, SAT-GAN tries to recognize error data as outliers by learning the latent features of clean data. In our evaluation, SAT-GAN achieves an average F1-score of 0.95 on five datasets, which yields at least 46.2% F1-score improvement over rule-based methods and outperforms state-of-the-art deep learning approaches in the absence of rules and negative samples.
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http://dx.doi.org/10.3390/e24070936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317027PMC
July 2022

Transcriptome Analysis of Bulbs Reveals Flowering in the Age-Mediated Pathway.

Biomolecules 2022 06 27;12(7). Epub 2022 Jun 27.

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.

is a summer bulbous flower that commonly needs to go through a long period of vegetative growth for 3 to 5 years before flowering. Plant flowering is regulated by a complex genetic network. Compared with most perennial flowers, knowledge on the molecular mechanism responsible for floral transition in bulbous flowers is lacking, and only a few genes that regulate flowering have been identified with few reports on the floral transition in . In this study, we identified many differentially expressed genes (DEGs) and transcription factors (TFs) by RNA-Seq in bulbs of different ages, including one- to four-year-old nonflowering bulbs and four-year-old flowering bulbs. Some DEGs were enriched in Gene Ontology (GO) terms between the three- and four-year-old bulbs, and there most genes were enriched in terms of metabolic process and catalytic activity. In the four-year old bulbs, most of the DEGs that may be involved in flowering were classified under the GO term biological process, which was a totally different result from the vegetative bulbs. Some DEGs between flowering and nonflowering bulbs were enriched in plant hormone signal transduction, including the hormones auxin, cytokinin, abscisic acid, and ethylene, but no DEGs were enriched in the gibberellin pathway. Auxin is the main endogenous phytohormone involved in bulb growth and development, but cytokinin, abscisic acid, and ethylene were shown to increase in flowering bulbs. In addition, energy-metabolism-related genes maintain a high expression level in large bulbs, and some positive regulators (SPL, COL, and AP1) and early flowering genes were also shown to be highly expressed in the meristems of flowering bulbs. It suggested that sugar molecules may be the energy source that regulates the signal transduction of flowering by connecting with phytohormone signaling in . A total of 1911 TFs were identified and classified into 89 categories, where the top six families with the largest gene numbers were C2H2, NAC, AP2/ERF-ERF, C3H, MYB-related, and WRKY. Most DEGs were in the AP2/ERF-ERF family, and most of them were downregulated in 4-year-old flowering bulbs. A number of families were reported to be involved in plant flowering, including NAC, AP2/ERF, MYB, WRKY, bZIP, MADS, and NF-Y. These results can act as a genetic resource to aid in the explanation of the genetic mechanism responsible for the flowering of and other bulbous flowers.
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http://dx.doi.org/10.3390/biom12070899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312979PMC
June 2022

HERC6 is upregulated in peripheral blood mononuclear cells of patients with systemic lupus erythematosus and promotes the disease progression.

Autoimmunity 2022 Jul 26:1-9. Epub 2022 Jul 26.

Pediatric Department, The First Hospital of Yulin, Yulin, PR China.

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease. Peripheral blood mononuclear cells (PBMCs) are any peripheral blood cell with round nuclei, including lymphocytes (T cells, B cells) and monocytes, whose physicochemical properties are randomized by obvious immune changes, and are a potentially effective source of SLE blood test samples and therapeutic targets. This study aimed to explore the upregulation molecules of PBMCs in patients with SLE and to explore their biological role. Homologous to the E6-AP carboxyl terminus (HECT) and regulator of chromosome condensation 1 (RCC1)-like domain (RLD) containing E3 ubiquitin protein ligase family member 6 (HERC6) expression was found significantly upregulated in four Gene Expression Omnibus gene sets. Moreover, HERC6 expression was upregulated in PBMCs from SLE patients compared with that in PBMCs from normal donors. HERC6 was significantly associated with SLE clinical phenotypes such as complement C3 content, erythrocyte sedimentation rate, and SLE disease activity index. , knockdown of HERC6 inhibited PBMC apoptosis, inflammatory response, and janus kinase (JAK)/signal transducer and activator of transcription (STAT) signalling pathway, while overexpression of HERC6 led to the opposite results. In addition, AG490, a JAK/STAT pathway inhibitor, reversed the promoting effect of HERC6 overexpression on PBMC apoptosis and inflammation. In conclusion, the level of HERC6 in PBMCs in patients with SLE was upregulated. Overexpression of HERC6 promoted PBMC apoptosis and inflammatory response, which was involved in the JAK/STAT pathway.
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http://dx.doi.org/10.1080/08916934.2022.2103800DOI Listing
July 2022

Rhizosphere and Straw Return Interactively Shape Rhizosphere Bacterial Community Composition and Nitrogen Cycling in Paddy Soil.

Front Microbiol 2022 7;13:945927. Epub 2022 Jul 7.

Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture, P.R. China, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Currently, how rice roots interact with straw return in structuring rhizosphere communities and nitrogen (N) cycling functions is relatively unexplored. In this study, paddy soil was amended with wheat straw at 1 and 2% w/w and used for rice growth. The effects of the rhizosphere, straw, and their interaction on soil bacterial community composition and N-cycling gene abundances were assessed at the rice maturity stage. For the soil without straw addition, rice growth, i.e., the rhizosphere effect, significantly altered the bacterial community composition and abundances of N-cycling genes, such as archaeal and bacterial ( and ), , and . The comparison of bulk soils between control and straw treatments showed a shift in bacterial community composition and decreased abundance of , and , which were attributed to sole straw effects. The comparison of rhizosphere soils between control and straw treatments showed an increase in the gene and a decrease in the gene, which were attributed to the interaction of straw and the rhizosphere. The number of differentially abundant genera in bulk soils between control and straw treatments was 13-23, similar to the number of 16-22 genera in rhizosphere soil between control and straw treatment. However, the number of genera affected by the rhizosphere effect was much lower in soil amended with straw (3-4) than in soil without straw addition (9). Results suggest possibly more pronounced impacts of straw amendments in shaping soil bacterial community composition.
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http://dx.doi.org/10.3389/fmicb.2022.945927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301285PMC
July 2022

Evaluating Users' Emotional Experience in Mobile Libraries: An Emotional Model Based on the Pleasure-Arousal-Dominance Emotion Model and the Five Factor Model.

Front Psychol 2022 5;13:942198. Epub 2022 Jul 5.

School of Information Management, Wuhan University, Wuhan, China.

As a part of user experience, user emotion has rarely been studied in mobile libraries. Specifically, with the proposed emotional model in combination with the Pleasure-Arousal-Dominance (PAD) Emotion Model and the Five Factor Model (FFM), we evaluate user emotions on the mobile library's three IS features (i.e., user interface, interaction quality, and service environment). An experience procedure with three tasks has been designed to collect data. 50 participants were enrolled, and they were asked to fill in questionnaires right after the experience. The correlations among the PAD emotions were examined. Specifically, users have a low perception of pleasure (P), high perception of arousal (A), and low perception of dominance (D). However, these three emotional states were not always significantly correlated with each other. This study extends mobile library research by focusing on users' emotional experience. Specifically, the detailed PAD emotions have been examined. This study provides a new approach for application developers and managers to evaluate the user experience of an application.
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http://dx.doi.org/10.3389/fpsyg.2022.942198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296843PMC
July 2022

Identification of circRNA-miRNA-mRNA Regulatory Network and Crucial Signaling Pathway Axis Involved in Tetralogy of Fallot.

Front Genet 2022 7;13:917454. Epub 2022 Jul 7.

Institute for Literature and Culture of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.

Tetralogy of Fallot (TOF) is one of the most common cyanotic congenital heart diseases (CHD) worldwide; however, its pathogenesis remains unclear. Recent studies have shown that circular RNAs (circRNAs) act as "sponges" for microRNAs (miRNAs) to compete for endogenous RNA (ceRNA) and play important roles in regulating gene transcription and biological processes. However, the mechanism of ceRNA in TOF remains unclear. To explore the crucial regulatory connections and pathways of TOF, we obtained the human TOF gene, miRNA, and circRNA expression profiling datasets from the Gene Expression Omnibus (GEO) database. After data pretreatment, differentially expressed mRNAs (DEmRNAs), microRNAs (DEmiRNAs), and circRNAs (DEcircRNAs) were identified between the TOF and healthy groups, and a global triple ceRNA regulatory network, including circRNAs, miRNAs, and mRNAs based on the integrated data, was constructed. A functional enrichment analysis was performed on the Metascape website to explore the biological functions of the selected genes. Then, we constructed a protein-protein interaction (PPI) network and identified seven hub genes using the cytoHubba and MCODE plug-ins in the Cytoscape software, including BCL2L11, PIK3R1, SOCS3, OSMR, STAT3, RUNX3, and IL6R. Additionally, a circRNA-miRNA-hub gene subnetwork was established, and its enrichment analysis results indicated that the extrinsic apoptotic signaling pathway, JAK-STAT signaling pathway and PI3K-Akt signaling pathway may be involved in the pathogenesis of TOF. We further identified the hsa_circ_000601/hsa-miR-148a/BCL2L11 axis as a crucial signaling pathway axis from the subnetwork. This study provides a novel regulatory network for the pathogenesis of TOF, revealing the possible molecular mechanisms and crucial regulatory pathways that may provide new strategies for candidate diagnostic biomarkers or potential therapeutic targets for TOF.
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http://dx.doi.org/10.3389/fgene.2022.917454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300927PMC
July 2022

Fully-Exposed Pd Cluster Catalyst: An Excellent Catalytic Antibacterial Nanomaterial.

Small 2022 Jul 24:e2203283. Epub 2022 Jul 24.

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering and College of Engineering, and BIC-ESAT, Peking University, Beijing, 100871, P. R. China.

Exploring antibacterial nanomaterials with excellent catalytic antibacterial properties has always been a hot research topic. However, the construction of nanomaterials with robust antibacterial activity at the atomic level remains a great challenge. Here a fully-exposed Pd cluster atomically-dispersed on nanodiamond-graphene (Pd /[email protected]) with excellent catalytic antibacterial properties is reported. The fully-exposed Pd cluster nanozyme provides atomically-dispersed Pd cluster sites that facilitate the activation of oxygen. Notably, the oxidase-like catalytic performance of the fully-exposed Pd cluster nanozyme is much higher than that of Pd single-atom oxidase mimic, Pd nanoparticles oxidase mimic and even the previously reported palladium-based oxidase mimics. Under the density functional theory (DFT) calculations, the Pd cluster sites can efficiently catalyze the decomposition of oxygen to generate reactive oxygen species, resulting in strong antibacterial properties. This research provides a valuable insight to the design of novel oxidase mimic and antibacterial nanomaterial.
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http://dx.doi.org/10.1002/smll.202203283DOI Listing
July 2022

Replication stress impairs chromosome segregation and preimplantation development in human embryos.

Cell 2022 Aug 19;185(16):2988-3007.e20. Epub 2022 Jul 19.

Department of Obstetrics and Gynecology, Columbia University, New York, NY 10032, USA; Department of Pediatrics and Naomi Berrie Diabetes Center, Columbia Stem Cell Initiative, Columbia University, New York, NY 10032, USA. Electronic address:

Human cleavage-stage embryos frequently acquire chromosomal aneuploidies during mitosis due to unknown mechanisms. Here, we show that S phase at the 1-cell stage shows replication fork stalling, low fork speed, and DNA synthesis extending into G2 phase. DNA damage foci consistent with collapsed replication forks, DSBs, and incomplete replication form in G2 in an ATR- and MRE11-dependent manner, followed by spontaneous chromosome breakage and segmental aneuploidies. Entry into mitosis with incomplete replication results in chromosome breakage, whole and segmental chromosome errors, micronucleation, chromosome fragmentation, and poor embryo quality. Sites of spontaneous chromosome breakage are concordant with sites of DNA synthesis in G2 phase, locating to gene-poor regions with long neural genes, which are transcriptionally silent at this stage of development. Thus, DNA replication stress in mammalian preimplantation embryos predisposes gene-poor regions to fragility, and in particular in the human embryo, to the formation of aneuploidies, impairing developmental potential.
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http://dx.doi.org/10.1016/j.cell.2022.06.028DOI Listing
August 2022

α-Synuclein fibril-specific nanobody reduces prion-like α-synuclein spreading in mice.

Nat Commun 2022 Jul 19;13(1):4060. Epub 2022 Jul 19.

Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

Pathogenic α-synuclein (α-syn) is a prion-like protein that drives the pathogenesis of Lewy Body Dementia (LBD) and Parkinson's Disease (PD). To target pathogenic α-syn preformed fibrils (PFF), here we designed extracellular disulfide bond-free synthetic nanobody libraries in yeast. Following selection, we identified a nanobody, PFFNB2, that can specifically recognize α-syn PFF over α-syn monomers. PFFNB2 cannot inhibit the aggregation of α-syn monomer, but can significantly dissociate α-syn fibrils. Furthermore, adeno-associated virus (AAV)-encoding EGFP fused to PFFNB2 (AAV-EGFP-PFFNB2) can inhibit PFF-induced α-syn serine 129 phosphorylation (pS129) in mouse primary cortical neurons, and prevent α-syn pathology spreading to the cortex in the transgenic mice expressing human wild type (WT) α-syn by intrastriatal-PFF injection. The pS129 immunoreactivity is negatively correlated with the expression of AAV-EGFP-PFFNB2. In conclusion, PFFNB2 holds a promise for mechanistic exploration and therapeutic development in α-syn-related pathogenesis.
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http://dx.doi.org/10.1038/s41467-022-31787-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296447PMC
July 2022

Sulfur-doped graphitic carbon nitride nanosheets as a sensitive fluorescent probe for detecting environmental and intracellular Ag.

Methods Appl Fluoresc 2022 Jul 27;10(4). Epub 2022 Jul 27.

Bristol Chinese Christian Church, c/o Tyndale Baptist Church, 137-139 Whiteladies Road, Bristol, BS8 2QG, United Kingdom.

Silver is widely used in medical materials, photography, electronics and other industries as a precious metal. The large-scale industrial production of silver-containing products and liquid waste emissions aggravate the environmental pollution. Silver ion is one of the most toxic metal ions, causing pollution to the environment and damage to public health. Therefore, the efficient and sensitive detection of Agin the water environment is extremely important. Sulfur-doped carbon nitride nanosheets (SCN Ns) were prepared by melamine and thiourea via high-temperature calcination. The morphology, chemical composition and surface functional groups of the SCN Ns were characterized by SEM, TEM, XRD, XPS, and FT-IR. The fluorescence of SCN Ns was gradually quenched as the Agconcentration increased. The detection limit for Agwas as low as 0.28 nM. The quenching mechanism mainly is attributed to static quenching. In this paper, SCN Ns were used as the fluorescent probe for detecting Ag. SCN Ns have successfully detected Agin different environmental aqueous samples and cells. Finally, SCN Ns were further applied to the visual quantitative detection of intracellular Ag.
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http://dx.doi.org/10.1088/2050-6120/ac8223DOI Listing
July 2022
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